MD09G1282900.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003430
Physical Location & Seq
Reverse (-)
36006922 .. 36009555
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1282900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGGAGTGCTGTCGAACAAGATCCAGAGGGATCTTCTGAAACCTGGAGATCACATCTATTCATGGCGCCACGCCTACCTCTATTCCCACCACGGGATATACTTTGGTGAAGGAAAGGTTATTCACTTCACTCGAGGAGCTGGCCAGGAAACTGGCACCGGAACTGTGTTAGACCGCTTCCTTCTAAGCAGCTCTCCATCCCATTCTTCGGACAATCCCTGCCCAAACTGCGGAGACCAATCGACACTTGATGGTGTCATCTCTTCTTGCATAGATTGTTTTCTATCAGGCGGTTATCTTTACCTCTTTGAATATGGTGTCAATCCAGCTTTCTTTATTGCCAAAGCTAGAGGGGGCACCTGTACCCTTGCCCGGTCTGACCCACCCGAAGATGTCCTTCATCGCGCTTCTTTCCTTCTGCAGAATGGTTTTGGTAGTTATGACATTTTCAAAAACAACTGCGAAGACTTTGCTATATATTGCAAAACAGGTTTGCTCGTGTTCACTAGCATTAGTGTGGGCAGGAGTGGACAGGCAGCCTCCATTGTAGCTGCCGCAACAGCTGTTGTTTCTTCACCGCTTCGCTTTTTGACTACCAGTTTTACTGGTCTGGGTGTTGTAGGCTGTGGCATGTATTGCATTAGTAGAATTGTTTCTGATATCGGAATACGCCGAGATGTTAAGAAAGTACCGGTGGAGAGACTGATTACCCCCTGTGCCCTAGACGAGTCAGTGGTTGCAACTGAAGTGCTCAAGAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

255

Amino Acids

27.44

Weight (kDa)

7.02

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 13 - 166 9.3e-29 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 66, 155, 356
AccII CGCG 1 cut(s) 405
AciI CCGC 5 cut(s) 175, 231, 291, 555, 578
AclWI GGATC 2 cut(s) 16, 39
AcoI YGGCCR 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 364, 690
AfiI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
AgeI ACCGGT 1 cut(s) 691
AgsI TTSAA 2 cut(s) 311, 451
AjnI CCWGG 2 cut(s) 43, 144
AluBI AGCT 6 cut(s) 140, 192, 329, 347, 551, 563
AluI AGCT 6 cut(s) 140, 192, 329, 347, 551, 563
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 2 cut(s) 228, 694
AlwI GGATC 2 cut(s) 16, 39
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 3 cut(s) 189, 536, 551
AsiGI ACCGGT 1 cut(s) 691
Asp700I GAANNNNTTC 1 cut(s) 652
AspLEI GCGC 2 cut(s) 69, 407
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 119, 567
AvaI CYCGRG 1 cut(s) 132
BaeGI GKGCMC 2 cut(s) 359, 721
BalI TGGCCA 1 cut(s) 144
BanI GGYRCC 3 cut(s) 66, 155, 356
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 753
BbvI GCAGC 3 cut(s) 201, 538, 548
BccI CCATC 2 cut(s) 205, 245
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 2 cut(s) 45, 146
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 228, 694
BfaI CTAG 3 cut(s) 348, 507, 722
BfmI CTRYAG 1 cut(s) 419
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 4 cut(s) 190, 537, 552, 555
BlsI GCNGC 4 cut(s) 191, 538, 553, 556
Bme1390I CCNGG 3 cut(s) 45, 146, 373
BmeT110I CYCGRG 1 cut(s) 132
BmiI GGNNCC 3 cut(s) 68, 157, 358
BmrFI CCNGG 3 cut(s) 45, 146, 373
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 2 cut(s) 158, 691
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 3 cut(s) 157, 597, 610
BseBI CCWGG 2 cut(s) 45, 146
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
BseNI ACTGG 3 cut(s) 157, 597, 610
BseRI GAGGAG 1 cut(s) 150
BseSI GKGCMC 2 cut(s) 359, 721
BseXI GCAGC 3 cut(s) 201, 538, 548
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 3 cut(s) 66, 155, 356
BshTI ACCGGT 1 cut(s) 691
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 3 cut(s) 159, 373, 692
BslI CCNNNNNNNGG 4 cut(s) 93, 208, 230, 372
BsmAI GTCTC 2 cut(s) 228, 694
BsnI GGCC 1 cut(s) 144
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 3 cut(s) 359, 721, 753
Bsp143I GATC 3 cut(s) 21, 31, 49
BspACI CCGC 5 cut(s) 175, 231, 291, 555, 578
BspANI GGCC 1 cut(s) 144
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 68, 157, 358
BspMAI CTGCAG 1 cut(s) 423
BspPI GGATC 2 cut(s) 16, 39
BspT107I GGYRCC 3 cut(s) 66, 155, 356
BspTNI GGTCTC 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 3 cut(s) 157, 597, 610
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 21, 31, 49
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 2 cut(s) 45, 146
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 268
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 185
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 2 cut(s) 69, 407
BstKTI GATC 3 cut(s) 24, 34, 52
BstMAI GTCTC 2 cut(s) 228, 694
BstMBI GATC 3 cut(s) 21, 31, 49
BstMWI GCNNNNNNNGC 3 cut(s) 228, 560, 636
BstNI CCWGG 2 cut(s) 45, 146
BstNSI RCATGY 1 cut(s) 634
BstSCI CCNGG 3 cut(s) 43, 144, 371
BstSFI CTRYAG 1 cut(s) 419
BstSLI GKGCMC 2 cut(s) 359, 721
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 3 cut(s) 201, 538, 548
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 2 cut(s) 21, 31
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 2 cut(s) 21, 31
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 738
Cac8I GCNNGC 1 cut(s) 142
CfoI GCGC 2 cut(s) 69, 407
Cfr10I RCCGGY 1 cut(s) 691
Csp6I GTAC 2 cut(s) 363, 689
CspAI ACCGGT 1 cut(s) 691
CviAII CATG 2 cut(s) 63, 631
CviJI RGCY 9 cut(s) 140, 144, 192, 329, 347, 539, 551, 563, 624
CviKI_1 RGCY 9 cut(s) 140, 144, 192, 329, 347, 539, 551, 563, 624
CviQI GTAC 2 cut(s) 363, 689
DdeI CTNAG 1 cut(s) 185
DinI GGCGCC 1 cut(s) 68
DpnI GATC 3 cut(s) 23, 33, 51
DpnII GATC 3 cut(s) 21, 31, 49
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 765
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 43, 144
EcoRV GATATC 1 cut(s) 661
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
FaeI CATG 2 cut(s) 66, 634
FaiI YATR 8 cut(s) 64, 100, 272, 315, 441, 476, 478, 632
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 62, 630
Fnu4HI GCNGC 4 cut(s) 190, 537, 552, 555
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 190, 537, 552, 555
FspBI CTAG 3 cut(s) 348, 507, 722
GlaI GCGC 2 cut(s) 68, 406
GluI GCNGC 4 cut(s) 190, 537, 552, 555
GsuI CTGGAG 1 cut(s) 66
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 1 cut(s) 144
HapII CCGG 3 cut(s) 159, 373, 692
HhaI GCGC 2 cut(s) 69, 407
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 2 cut(s) 66, 634
Hin6I GCGC 2 cut(s) 67, 405
HinP1I GCGC 2 cut(s) 67, 405
HinfI GANTC 1 cut(s) 728
HpaII CCGG 3 cut(s) 159, 373, 692
HphI GGTGA 2 cut(s) 119, 567
Hpy166II GTNNAC 2 cut(s) 504, 530
Hpy188I TCNGA 5 cut(s) 39, 211, 379, 658, 665
Hpy188III TCNNGA 2 cut(s) 25, 754
Hpy8I GTNNAC 2 cut(s) 504, 530
HpyAV CCTTC 4 cut(s) 104, 191, 407, 425
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 270, 421, 483, 639, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 560, 636
HpyF3I CTNAG 1 cut(s) 185
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 2 cut(s) 66, 634
HspAI GCGC 2 cut(s) 67, 405
KasI GGCGCC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 21, 31, 49
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 3 cut(s) 201, 538, 548
MaeI CTAG 3 cut(s) 348, 507, 722
MalI GATC 3 cut(s) 23, 33, 51
MboI GATC 3 cut(s) 21, 31, 49
MboII GAAGA 6 cut(s) 26, 198, 255, 401, 476, 564
MflI RGATCY 2 cut(s) 21, 31
MhlI GDGCHC 3 cut(s) 359, 721, 753
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 6 cut(s) 21, 89, 128, 314, 344, 550
Mox20I TGGCCA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 652
MscI TGGCCA 1 cut(s) 144
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 515
Msp20I TGGCCA 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 3 cut(s) 159, 373, 692
MspR9I CCNGG 3 cut(s) 45, 146, 373
MvaI CCWGG 2 cut(s) 45, 146
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 3 cut(s) 228, 560, 636
NarI GGCGCC 1 cut(s) 67
NciI CCSGG 1 cut(s) 373
NdeII GATC 3 cut(s) 21, 31, 49
NlaIII CATG 2 cut(s) 66, 634
NlaIV GGNNCC 3 cut(s) 68, 157, 358
NmeAIII GCCGAG 1 cut(s) 698
NspI RCATGY 1 cut(s) 634
PaeR7I CTCGAG 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 652
PinAI ACCGGT 1 cut(s) 691
PkrI GCNGC 4 cut(s) 191, 538, 553, 556
PleI GAGTC 1 cut(s) 736
PluTI GGCGCC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 736
Psp6I CCWGG 2 cut(s) 43, 144
PspGI CCWGG 2 cut(s) 43, 144
PspN4I GGNNCC 3 cut(s) 68, 157, 358
PspXI VCTCGAGB 1 cut(s) 132
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 2 cut(s) 21, 31
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 2 cut(s) 364, 690
RsaNI GTAC 2 cut(s) 363, 689
RseI CAYNNNNRTG 1 cut(s) 515
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 4 cut(s) 190, 537, 552, 555
Sau3AI GATC 3 cut(s) 21, 31, 49
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 3 cut(s) 45, 146, 373
SduI GDGCHC 3 cut(s) 359, 721, 753
SfcI CTRYAG 1 cut(s) 419
SfoI GGCGCC 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 2 cut(s) 132, 752
SmoI CTYRAG 2 cut(s) 132, 752
Sse9I AATT 1 cut(s) 648
SsiI CCGC 5 cut(s) 175, 231, 291, 555, 578
SspDI GGCGCC 1 cut(s) 66
SspMI CTAG 3 cut(s) 348, 507, 722
StyD4I CCNGG 3 cut(s) 43, 144, 371
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 3 cut(s) 14, 133, 242
TasI AATT 1 cut(s) 648
TauI GCSGC 1 cut(s) 557
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 1 cut(s) 738
TseI GCWGC 3 cut(s) 189, 536, 551
TspDTI ATGAA 2 cut(s) 51, 389
TspRI CASTG 1 cut(s) 738
XceI RCATGY 1 cut(s) 634
XhoI CTCGAG 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 652
XspI CTAG 3 cut(s) 348, 507, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.