Rroxscaffold_6G00394640

NC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003430
Physical Location & Seq
Reverse (-)
15404263 .. 15408361
4099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394640.1

Sequence Viewer

Length: 783 bp
ATGGGTGTGCTGTCAAACAAGATCCACAGGGATCAACTCAAACCTGGCGATCATATCTATACATGGAGAAACGCTTACCTCTATGCCCATCACGGGATATATGTCGGTGAGGAAAAGGTTATTCACTTCACTCGAGCAGCTGGAAGGGAAATTGGCACAGGAACTGTGTTAGACCGTTTCATTTTCAGCTCATCTCCATCCCATTCTTCAGAGAATCCCTGCACGAGTTGCGATGATCCATCAGGGCGTGATGGCGGTGTCATGTCTTCTTGCCTAGATTGTTTTCTGTCTGGTGGTGATCTGTACCGCTTTGAGTATGGTGTCACAAAAACCTTCTTTATTGCGAAAGCCAGAGGAGGTACCTGTACCCTTGCTTCGTCTGATCCATATGAAGATGTCTGTCACCGTGCATTTTACCTTCTCAAGAAGAAGAATGGTTTTGGCAGCTACAGCCTTTTCAAAAACAATTGTGAAGACTTTGCAATTTATTGCAAAACAGGTTTGCTTGTGAATAGTAGCATTGGTTTGGGAAGGAGTGGCCAGGCATCCTCCGGTTTCTCTTTTGCAGGTGTTGCAACTGGTATCGGTTGTTCGTCAGCTCGATATTTGCCTACTATGAGTGGTCTGAGTGCTGTCGGTGCTTTCAGTGGTCTGGGTGTTGCTTTCGGTGCTGGAATTTATTCTCTCCAGCGATTGGCTTATGATATTGGATTCCGCAGTGATGTTTCAAAAGTGTCAGTGGAGGAACTCTGGGACTATATGAATAAAAACCAGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

260

Amino Acids

28.19

Weight (kDa)

8.5

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 169 1.5e-27 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 557
Acc36I ACCTGC 1 cut(s) 557
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 694
AciI CCGC 3 cut(s) 255, 307, 715
AclWI GGATC 4 cut(s) 16, 39, 230, 377
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 3 cut(s) 305, 361, 367
AfiI CCNNNNNNNGG 2 cut(s) 93, 694
AgsI TTSAA 2 cut(s) 460, 729
AjnI CCWGG 2 cut(s) 43, 540
AluBI AGCT 4 cut(s) 140, 189, 447, 599
AluI AGCT 4 cut(s) 140, 189, 447, 599
AlwI GGATC 4 cut(s) 16, 39, 230, 377
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 538
ApeKI GCWGC 2 cut(s) 137, 444
ApoI RAATTY 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 679
Asp718I GGTACC 1 cut(s) 359
AsuHPI GGTGA 3 cut(s) 119, 308, 395
AvaI CYCGRG 1 cut(s) 132
BalI TGGCCA 1 cut(s) 540
BanI GGYRCC 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 358, 390
BauI CACGAG 1 cut(s) 223
BbsI GAAGAC 2 cut(s) 258, 480
BbvI GCAGC 2 cut(s) 149, 456
BccI CCATC 4 cut(s) 96, 205, 245, 247
BciT130I CCWGG 2 cut(s) 45, 542
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 557
BisI GCNGC 2 cut(s) 138, 445
BlsI GCNGC 2 cut(s) 139, 446
Bme1390I CCNGG 2 cut(s) 45, 542
BmeT110I CYCGRG 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 2 cut(s) 45, 542
BmsI GCATC 1 cut(s) 554
BpiI GAAGAC 2 cut(s) 258, 480
BpmI CTGGAG 1 cut(s) 671
BpuEI CTTGAG 1 cut(s) 407
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 694
Bse1I ACTGG 1 cut(s) 583
BseBI CCWGG 2 cut(s) 45, 542
BseGI GGATG 2 cut(s) 197, 545
BseLI CCNNNNNNNGG 2 cut(s) 93, 694
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 583
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 149, 456
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 1 cut(s) 540
BshNI GGYRCC 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 552
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 2 cut(s) 93, 694
BsmFI GGGAC 1 cut(s) 767
BsnI GGCC 1 cut(s) 540
BsoBI CYCGRG 1 cut(s) 132
Bsp143I GATC 6 cut(s) 21, 31, 49, 235, 298, 382
BspACI CCGC 3 cut(s) 255, 307, 715
BspANI GGCC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 618
BspLI GGNNCC 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 557
BspPI GGATC 4 cut(s) 16, 39, 230, 377
BspT107I GGYRCC 1 cut(s) 359
BsrI ACTGG 1 cut(s) 583
BssMI GATC 6 cut(s) 21, 31, 49, 235, 298, 382
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 2 cut(s) 45, 542
Bst4CI ACNGT 3 cut(s) 166, 176, 407
BstAPI GCANNNNNTGC 2 cut(s) 228, 572
BstDEI CTNAG 1 cut(s) 626
BstF5I GGATG 2 cut(s) 197, 545
BstKTI GATC 6 cut(s) 24, 34, 52, 238, 301, 385
BstMBI GATC 6 cut(s) 21, 31, 49, 235, 298, 382
BstMWI GCNNNNNNNGC 5 cut(s) 228, 450, 572, 638, 668
BstNI CCWGG 2 cut(s) 45, 542
BstSCI CCNGG 2 cut(s) 43, 540
BstSFI CTRYAG 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 149, 456
BstV2I GAAGAC 2 cut(s) 258, 480
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 1 cut(s) 540
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 197, 545
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 3 cut(s) 652, 724, 744
BveI ACCTGC 1 cut(s) 557
CaiI CAGNNNCTG 1 cut(s) 164
Csp6I GTAC 3 cut(s) 304, 360, 366
CviAII CATG 2 cut(s) 63, 262
CviJI RGCY 8 cut(s) 140, 189, 350, 447, 453, 540, 599, 698
CviKI_1 RGCY 8 cut(s) 140, 189, 350, 447, 453, 540, 599, 698
CviQI GTAC 3 cut(s) 304, 360, 366
DdeI CTNAG 1 cut(s) 626
DpnI GATC 6 cut(s) 23, 33, 51, 237, 300, 384
DpnII GATC 6 cut(s) 21, 31, 49, 235, 298, 382
EaeI YGGCCR 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 43, 540
FaeI CATG 2 cut(s) 66, 265
FaqI GGGAC 1 cut(s) 767
FatI CATG 2 cut(s) 62, 261
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 138, 445
FokI GGATG 2 cut(s) 184, 532
Fsp4HI GCNGC 2 cut(s) 138, 445
FspBI CTAG 1 cut(s) 275
GluI GCNGC 2 cut(s) 138, 445
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 1 cut(s) 540
HapII CCGG 1 cut(s) 552
Hin1II CATG 2 cut(s) 66, 265
HinfI GANTC 2 cut(s) 214, 711
HpaII CCGG 1 cut(s) 552
HphI GGTGA 3 cut(s) 119, 308, 395
Hpy188I TCNGA 3 cut(s) 211, 382, 627
Hpy188III TCNNGA 1 cut(s) 424
HpyAV CCTTC 4 cut(s) 138, 343, 428, 525
HpyCH4III ACNGT 3 cut(s) 166, 176, 407
HpyCH4V TGCA 6 cut(s) 222, 410, 482, 492, 566, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 228, 450, 572, 638, 668
HpyF3I CTNAG 1 cut(s) 626
Hsp92II CATG 2 cut(s) 66, 265
KpnI GGTACC 1 cut(s) 363
Kzo9I GATC 6 cut(s) 21, 31, 49, 235, 298, 382
Lsp1109I GCAGC 2 cut(s) 149, 456
LweI GCATC 1 cut(s) 554
MaeI CTAG 1 cut(s) 275
MaeIII GTNAC 2 cut(s) 322, 401
MalI GATC 6 cut(s) 23, 33, 51, 237, 300, 384
MboI GATC 6 cut(s) 21, 31, 49, 235, 298, 382
MboII GAAGA 6 cut(s) 198, 258, 404, 439, 442, 485
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 4 cut(s) 150, 466, 483, 675
MluNI TGGCCA 1 cut(s) 540
MnlI CCTC 6 cut(s) 89, 103, 347, 350, 559, 736
Mox20I TGGCCA 1 cut(s) 540
MroXI GAANNNNTTC 1 cut(s) 679
MscI TGGCCA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 552
MspR9I CCNGG 2 cut(s) 45, 542
MunI CAATTG 1 cut(s) 466
MvaI CCWGG 2 cut(s) 45, 542
MwoI GCNNNNNNNGC 5 cut(s) 228, 450, 572, 638, 668
NdeI CATATG 1 cut(s) 388
NdeII GATC 6 cut(s) 21, 31, 49, 235, 298, 382
NlaIII CATG 2 cut(s) 66, 265
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 322, 401
PaeR7I CTCGAG 1 cut(s) 132
PaqCI CACCTGC 1 cut(s) 557
PdmI GAANNNNTTC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 214, 711
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 2 cut(s) 139, 446
Psp6I CCWGG 2 cut(s) 43, 540
PspGI CCWGG 2 cut(s) 43, 540
PspN4I GGNNCC 1 cut(s) 361
PspXI VCTCGAGB 1 cut(s) 132
PsrI GAACNNNNNNTAC 2 cut(s) 574, 606
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 3 cut(s) 305, 361, 367
RsaNI GTAC 3 cut(s) 304, 360, 366
SatI GCNGC 2 cut(s) 138, 445
Sau3AI GATC 6 cut(s) 21, 31, 49, 235, 298, 382
ScrFI CCNGG 2 cut(s) 45, 542
SfaNI GCATC 1 cut(s) 554
SfcI CTRYAG 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmlI CTYRAG 2 cut(s) 132, 422
SmoI CTYRAG 2 cut(s) 132, 422
Sse9I AATT 4 cut(s) 150, 466, 483, 675
SsiI CCGC 3 cut(s) 255, 307, 715
SspMI CTAG 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 43, 540
TaaI ACNGT 3 cut(s) 166, 176, 407
TaqI TCGA 2 cut(s) 133, 601
TasI AATT 4 cut(s) 150, 466, 483, 675
TfiI GAWTC 2 cut(s) 214, 711
TscAI CASTG 3 cut(s) 652, 724, 744
TseFI GTSAC 2 cut(s) 322, 401
TseI GCWGC 2 cut(s) 137, 444
Tsp45I GTSAC 2 cut(s) 322, 401
TspDTI ATGAA 3 cut(s) 169, 405, 776
TspRI CASTG 3 cut(s) 652, 724, 744
Van91I CCANNNNNTGG 1 cut(s) 694
XapI RAATTY 1 cut(s) 675
XhoI CTCGAG 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 679
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.