Rroxscaffold_6G00394620

NC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003430
Physical Location & Seq
Reverse (-)
15372706 .. 15376791
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394620.1

Sequence Viewer

Length: 795 bp
ATGGGTTTGCTGTCAAACAAGATCCACAGGGACCAACTCAAACCTGGTGATCATATCTATTCATGGAGGAACGCCTACCTCTATGCCCATCACGGTCACCATGTCTGCCATATCACCTCAGGGATATATGTCGGTGAGGGAAAGGTTATCCACTTCACTCGAGCAGCTGGGCAGGAAATTGGCACAGGAACTGTGTTAGACCGTTTCATTTTCAGCTCATCTCCATCCCATTCTTCAGAGAATCCCTGCACGAGTTGCGATGATCCATCAGGGCCTGATGGTGGTGTCATGTCTTCTTGCCTAGATTGTTTTCTGTCTGGTGGTGATCTGTACCGCTTTGAATATGGTGTCAATCCTGCTTTCTTTATTGCCAAAGCCAGAGGAGGTACCTGTACCCTTGCCTCGTCTGACCCATCTGAAGATGTCCTTCACCGTGCCTTTTTCCTTCTCCAGAACGGTTTTGGGAGCTACTGCCTTTTCAAAAACAATTGTGAAGACTTTGCAATTTATTGCAAAACAGGTTTGCTTGTGTTTACTAGCATTAGTGTGGGAAGGAGTGGGCAGGCAGCCTCCATTTTCTCTGCTGCAAGTGCTATTGCTTGTTCACCGGCCCGATATTTGACTACTACTTTTAGTGGTCTGAGTGCTTTCGGTGCTGGAATTTATTGTCTCCATCGATTGGTTTGTGATATTGGAATCCGCCGTGATGTTTCAAAAGTGCCAGTTGAGAGACTAGTTGATCGGGCTGGCTTCGTCGAGTCGGAGGTTGCAACTGAGATGGTCAAGCGAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

264

Amino Acids

28.58

Weight (kDa)

6.58

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 176 2.7e-22 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 679
AciI CCGC 2 cut(s) 334, 700
AclWI GGATC 2 cut(s) 16, 257
AcsI RAATTY 1 cut(s) 660
AcuI CTGAAG 2 cut(s) 219, 438
AfaI GTAC 3 cut(s) 332, 388, 394
AfiI CCNNNNNNNGG 2 cut(s) 281, 679
AgsI TTSAA 3 cut(s) 341, 481, 714
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 3 cut(s) 167, 216, 468
AluI AGCT 3 cut(s) 167, 216, 468
Alw26I GTCTC 2 cut(s) 674, 724
AlwI GGATC 2 cut(s) 16, 257
AlwNI CAGNNNCTG 2 cut(s) 191, 275
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 2 cut(s) 272, 609
ApeKI GCWGC 3 cut(s) 164, 566, 584
ApoI RAATTY 1 cut(s) 660
Asp718I GGTACC 1 cut(s) 386
AspS9I GGNCC 3 cut(s) 31, 272, 610
AsuHPI GGTGA 7 cut(s) 59, 89, 106, 146, 335, 422, 597
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 31
AxyI CCTNAGG 1 cut(s) 118
BanI GGYRCC 1 cut(s) 386
BauI CACGAG 1 cut(s) 250
BbsI GAAGAC 2 cut(s) 285, 501
BbvI GCAGC 3 cut(s) 176, 571, 578
BccI CCATC 7 cut(s) 96, 232, 272, 274, 421, 681, 772
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 674, 724
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 3 cut(s) 302, 537, 734
BisI GCNGC 3 cut(s) 165, 567, 585
BlsI GCNGC 3 cut(s) 166, 568, 586
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 31, 272, 610
BmiI GGNNCC 2 cut(s) 32, 388
BmrFI CCNGG 1 cut(s) 45
BpiI GAAGAC 2 cut(s) 285, 501
BpmI CTGGAG 1 cut(s) 434
Bsa29I ATCGAT 1 cut(s) 676
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 679
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 722
Bse21I CCTNAGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 45
BseCI ATCGAT 1 cut(s) 676
BseGI GGATG 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 281, 679
BseMII CTCAG 3 cut(s) 132, 632, 765
BseNI ACTGG 1 cut(s) 722
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 3 cut(s) 176, 571, 578
BseYI CCCAGC 1 cut(s) 167
BsgI GTGCAG 1 cut(s) 232
BshFI GGCC 2 cut(s) 274, 611
BshNI GGYRCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 1 cut(s) 608
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 281, 679
BsmAI GTCTC 2 cut(s) 674, 724
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 2 cut(s) 274, 611
BsoBI CYCGRG 1 cut(s) 159
Bsp143I GATC 5 cut(s) 21, 49, 262, 325, 739
BspACI CCGC 2 cut(s) 334, 700
BspANI GGCC 2 cut(s) 274, 611
BspCNI CTCAG 3 cut(s) 131, 633, 766
BspDI ATCGAT 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 32, 388
BspPI GGATC 2 cut(s) 16, 257
BspT107I GGYRCC 1 cut(s) 386
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 1 cut(s) 722
BssAI RCCGGY 1 cut(s) 607
BssMI GATC 5 cut(s) 21, 49, 262, 325, 739
BssSI CACGAG 1 cut(s) 250
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 5 cut(s) 95, 193, 203, 434, 458
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 564, 748
BstDEI CTNAG 3 cut(s) 118, 641, 774
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 5 cut(s) 24, 52, 265, 328, 742
BstMAI GTCTC 2 cut(s) 674, 724
BstMBI GATC 5 cut(s) 21, 49, 262, 325, 739
BstMWI GCNNNNNNNGC 3 cut(s) 255, 590, 653
BstNI CCWGG 1 cut(s) 45
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 43
BstV1I GCAGC 3 cut(s) 176, 571, 578
BstV2I GAAGAC 2 cut(s) 285, 501
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 676
Bsu36I CCTNAGG 1 cut(s) 118
BsuRI GGCC 2 cut(s) 274, 611
BsuTUI ATCGAT 1 cut(s) 676
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 564, 748
CaiI CAGNNNCTG 2 cut(s) 191, 275
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 3 cut(s) 31, 272, 610
ClaI ATCGAT 1 cut(s) 676
CsiI ACCWGGT 1 cut(s) 43
Csp6I GTAC 3 cut(s) 331, 387, 393
CviAII CATG 3 cut(s) 63, 101, 289
CviJI RGCY 9 cut(s) 167, 216, 274, 377, 468, 569, 611, 746, 750
CviKI_1 RGCY 9 cut(s) 167, 216, 274, 377, 468, 569, 611, 746, 750
CviQI GTAC 3 cut(s) 331, 387, 393
DdeI CTNAG 3 cut(s) 118, 641, 774
DpnI GATC 5 cut(s) 23, 51, 264, 327, 741
DpnII GATC 5 cut(s) 21, 49, 262, 325, 739
EciI GGCGGA 1 cut(s) 689
Eco47I GGWCC 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 219, 438
Eco81I CCTNAGG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 159
Eco91I GGTNACC 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 272
EcoO65I GGTNACC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 3 cut(s) 66, 104, 292
FaiI YATR 9 cut(s) 54, 64, 84, 102, 111, 127, 129, 290, 345
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 1 cut(s) 44
FatI CATG 3 cut(s) 62, 100, 288
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 165, 567, 585
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 3 cut(s) 165, 567, 585
FspBI CTAG 3 cut(s) 302, 537, 734
GluI GCNGC 3 cut(s) 165, 567, 585
GsaI CCCAGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 2 cut(s) 274, 611
HapII CCGG 1 cut(s) 608
Hin1II CATG 3 cut(s) 66, 104, 292
HinfI GANTC 3 cut(s) 241, 696, 758
HpaII CCGG 1 cut(s) 608
HphI GGTGA 7 cut(s) 59, 89, 106, 146, 335, 422, 597
Hpy166II GTNNAC 2 cut(s) 534, 605
Hpy188I TCNGA 5 cut(s) 238, 409, 418, 642, 763
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 534, 605
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 3 cut(s) 437, 455, 546
HpyCH4III ACNGT 5 cut(s) 95, 193, 203, 434, 458
HpyCH4V TGCA 5 cut(s) 249, 503, 513, 587, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 590, 653
HpyF3I CTNAG 3 cut(s) 118, 641, 774
Hsp92II CATG 3 cut(s) 66, 104, 292
KpnI GGTACC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 5 cut(s) 21, 49, 262, 325, 739
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 3 cut(s) 176, 571, 578
MabI ACCWGGT 1 cut(s) 43
MaeI CTAG 3 cut(s) 302, 537, 734
MaeIII GTNAC 1 cut(s) 95
MalI GATC 5 cut(s) 23, 51, 264, 327, 741
MboI GATC 5 cut(s) 21, 49, 262, 325, 739
MboII GAAGA 4 cut(s) 225, 285, 431, 506
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 21
MluCI AATT 4 cut(s) 177, 487, 504, 660
MlyI GAGTC 1 cut(s) 767
MmeI TCCRAC 1 cut(s) 741
MnlI CCTC 9 cut(s) 60, 89, 127, 130, 374, 377, 412, 580, 757
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 1 cut(s) 45
MunI CAATTG 1 cut(s) 487
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 255, 590, 653
NdeII GATC 5 cut(s) 21, 49, 262, 325, 739
NlaIII CATG 3 cut(s) 66, 104, 292
NlaIV GGNNCC 2 cut(s) 32, 388
NmuCI GTSAC 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 159
PfeI GAWTC 2 cut(s) 241, 696
PflMI CCANNNNNTGG 1 cut(s) 679
PkrI GCNGC 3 cut(s) 166, 568, 586
PleI GAGTC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 766
Psp6I CCWGG 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 32, 388
PspPI GGNCC 3 cut(s) 31, 272, 610
PspXI VCTCGAGB 1 cut(s) 159
PstNI CAGNNNCTG 2 cut(s) 191, 275
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 3 cut(s) 332, 388, 394
RsaNI GTAC 3 cut(s) 331, 387, 393
RseI CAYNNNNRTG 1 cut(s) 545
SatI GCNGC 3 cut(s) 165, 567, 585
Sau3AI GATC 5 cut(s) 21, 49, 262, 325, 739
Sau96I GGNCC 3 cut(s) 31, 272, 610
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 1 cut(s) 45
SexAI ACCWGGT 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 159
SinI GGWCC 1 cut(s) 31
SlaI CTCGAG 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 4 cut(s) 177, 487, 504, 660
SsiI CCGC 2 cut(s) 334, 700
SspMI CTAG 3 cut(s) 302, 537, 734
StyD4I CCNGG 1 cut(s) 43
TaaI ACNGT 5 cut(s) 95, 193, 203, 434, 458
TaqI TCGA 3 cut(s) 160, 676, 756
TasI AATT 4 cut(s) 177, 487, 504, 660
TfiI GAWTC 2 cut(s) 241, 696
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 164, 566, 584
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 2 cut(s) 51, 196
Van91I CCANNNNNTGG 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 2 cut(s) 41, 458
XhoI CTCGAG 1 cut(s) 159
XspI CTAG 3 cut(s) 302, 537, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.