MD17G1272600.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003430
Physical Location & Seq
Reverse (-)
33441117 .. 33452653
11537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1272600.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGGAGTGCTGTCGAACAAGATCCGGAGGGATCTTCTGAAGCCTGGAGATCACATATATTCATGGCGCCGCGCCTACCTCTATGCCCACCACGGGATATATGTTGGTGAAGGAAAGGTTGTTCACTTCACTCGAGGAGCGGGCCAGGAAATTGGCACAGGAACTGTGCTAGACCGCTTCCTTGTAAGCAGCTCTCCATCGCACTCTCCGGACAATCCCTGCCCAAACTGTGATCAATCGACACTTGATGGTGTCATGTCTTCTTGCATAGATTGTTTTCTATCTGGCGGTGATCTTTACCTCTTTGAATATGGTGTCACTTCAGCTTTCTTTATTGCCAAGGCTAGAGGGGGTACCTGTACCCTTGCGCGGTCTGACCCTCCCGAAGATGTCCTTCACCGTGCTTCTTTCCTTCTGCAGAATGGCTTTGGTAGTTATGACATTTTCAAAAACAACTGTGAAGACTTCGCCATATATTGCAAAACAGGCTTGCTCGTGTTCACCAGCATTAGCGTGGGCAGGAGTGGACAGGCTGCCTCCTTCGTAGCTGCCGCAACTGCTGTTGTTTCTTCACCGCTTCGATTTATGACTACCAGTTTTACTGGTCTGGGTGCTGTAGGTTGTGGCATGTATTGTGTTAGTAGATTGGTTTCTGACATCGGAATACGCCGAGATGTTAGGAAAGTATCGGTGGAGAGACTGATTACCCCCTGTGCCCTAGACGAGTCAGTGGCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

248

Amino Acids

26.58

Weight (kDa)

7.54

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 13 - 165 6.3e-28 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 66, 353
AccBSI CCGCTC 1 cut(s) 140
AccII CGCG 2 cut(s) 72, 370
AccIII TCCGGA 2 cut(s) 24, 208
AciI CCGC 7 cut(s) 70, 140, 175, 288, 370, 552, 575
AclWI GGATC 2 cut(s) 16, 39
AcuI CTGAAG 2 cut(s) 59, 306
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 355, 361
AfiI CCNNNNNNNGG 2 cut(s) 93, 369
AgsI TTSAA 2 cut(s) 308, 448
AjnI CCWGG 2 cut(s) 43, 144
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 3 cut(s) 192, 326, 548
AluI AGCT 3 cut(s) 192, 326, 548
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 2 cut(s) 16, 39
AlwNI CAGNNNCTG 2 cut(s) 164, 734
Ama87I CYCGRG 1 cut(s) 132
Aor13HI TCCGGA 2 cut(s) 24, 208
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 4 cut(s) 189, 533, 548, 734
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 3 cut(s) 69, 74, 370
AspS9I GGNCC 1 cut(s) 142
AsuHPI GGTGA 5 cut(s) 119, 302, 389, 493, 564
AvaI CYCGRG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 718
BanI GGYRCC 2 cut(s) 66, 353
BauI CACGAG 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 252, 468
BbvI GCAGC 4 cut(s) 201, 520, 535, 721
BccI CCATC 2 cut(s) 205, 242
BciT130I CCWGG 2 cut(s) 45, 146
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 3 cut(s) 170, 345, 719
BfmI CTRYAG 2 cut(s) 416, 615
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 6 cut(s) 70, 190, 534, 549, 552, 735
BlsI GCNGC 6 cut(s) 71, 191, 535, 550, 553, 736
Bme1390I CCNGG 2 cut(s) 45, 146
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 68, 355
BmrFI CCNGG 2 cut(s) 45, 146
BpiI GAAGAC 2 cut(s) 252, 468
BpmI CTGGAG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 91, 339
BsaWI WCCGGW 2 cut(s) 24, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 369
Bse1I ACTGG 2 cut(s) 594, 607
BseAI TCCGGA 2 cut(s) 24, 208
BseBI CCWGG 2 cut(s) 45, 146
BseDI CCNNGG 2 cut(s) 91, 339
BseLI CCNNNNNNNGG 2 cut(s) 93, 369
BseNI ACTGG 2 cut(s) 594, 607
BseRI GAGGAG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 4 cut(s) 201, 520, 535, 721
Bsh1236I CGCG 2 cut(s) 72, 370
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 2 cut(s) 66, 353
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 2 cut(s) 25, 209
BslI CCNNNNNNNGG 2 cut(s) 93, 369
BsmAI GTCTC 1 cut(s) 691
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 718
Bsp13I TCCGGA 2 cut(s) 24, 208
Bsp143I GATC 5 cut(s) 21, 31, 49, 232, 292
BspACI CCGC 7 cut(s) 70, 140, 175, 288, 370, 552, 575
BspANI GGCC 1 cut(s) 144
BspEI TCCGGA 2 cut(s) 24, 208
BspFNI CGCG 2 cut(s) 72, 370
BspLI GGNNCC 2 cut(s) 68, 355
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 2 cut(s) 16, 39
BspT107I GGYRCC 2 cut(s) 66, 353
BsrBI CCGCTC 1 cut(s) 140
BsrI ACTGG 2 cut(s) 594, 607
BssECI CCNNGG 2 cut(s) 91, 339
BssMI GATC 5 cut(s) 21, 31, 49, 232, 292
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 1 cut(s) 339
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 2 cut(s) 45, 146
Bst4CI ACNGT 4 cut(s) 166, 230, 401, 458
BstACI GRCGYC 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 142, 491
BstDSI CCRYGG 1 cut(s) 91
BstFNI CGCG 2 cut(s) 72, 370
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 3 cut(s) 69, 74, 370
BstKTI GATC 5 cut(s) 24, 34, 52, 235, 295
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 5 cut(s) 21, 31, 49, 232, 292
BstMWI GCNNNNNNNGC 2 cut(s) 486, 557
BstNI CCWGG 2 cut(s) 45, 146
BstNSI RCATGY 1 cut(s) 631
BstSCI CCNGG 2 cut(s) 43, 144
BstSFI CTRYAG 2 cut(s) 416, 615
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 2 cut(s) 72, 370
BstV1I GCAGC 4 cut(s) 201, 520, 535, 721
BstV2I GAAGAC 2 cut(s) 252, 468
BstX2I RGATCY 2 cut(s) 21, 31
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 2 cut(s) 21, 31
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 2 cut(s) 142, 491
CaiI CAGNNNCTG 2 cut(s) 164, 734
CfoI GCGC 3 cut(s) 69, 74, 370
Cfr13I GGNCC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 354, 360
CviAII CATG 3 cut(s) 63, 256, 628
CviQI GTAC 2 cut(s) 354, 360
DinI GGCGCC 1 cut(s) 68
DpnI GATC 5 cut(s) 23, 33, 51, 234, 294
DpnII GATC 5 cut(s) 21, 31, 49, 232, 292
Eco130I CCWWGG 1 cut(s) 339
Eco57I CTGAAG 2 cut(s) 59, 306
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 43, 144
EcoT14I CCWWGG 1 cut(s) 339
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 3 cut(s) 66, 259, 631
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FatI CATG 3 cut(s) 62, 255, 627
FauI CCCGC 1 cut(s) 133
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 6 cut(s) 70, 190, 534, 549, 552, 735
Fsp4HI GCNGC 6 cut(s) 70, 190, 534, 549, 552, 735
FspBI CTAG 3 cut(s) 170, 345, 719
GlaI GCGC 3 cut(s) 68, 73, 369
GluI GCNGC 6 cut(s) 70, 190, 534, 549, 552, 735
GsuI CTGGAG 1 cut(s) 66
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 1 cut(s) 144
HapII CCGG 2 cut(s) 25, 209
HhaI GCGC 3 cut(s) 69, 74, 370
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 3 cut(s) 66, 259, 631
Hin6I GCGC 3 cut(s) 67, 72, 368
HinP1I GCGC 3 cut(s) 67, 72, 368
HinfI GANTC 1 cut(s) 725
HpaII CCGG 2 cut(s) 25, 209
HphI GGTGA 5 cut(s) 119, 302, 389, 493, 564
Hpy166II GTNNAC 3 cut(s) 124, 501, 527
Hpy188I TCNGA 4 cut(s) 39, 376, 655, 662
Hpy188III TCNNGA 3 cut(s) 25, 209, 383
Hpy8I GTNNAC 3 cut(s) 124, 501, 527
HpyAV CCTTC 4 cut(s) 104, 404, 422, 550
HpyCH4III ACNGT 4 cut(s) 166, 230, 401, 458
HpyCH4V TGCA 4 cut(s) 267, 418, 480, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 486, 557
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 3 cut(s) 66, 259, 631
HspAI GCGC 3 cut(s) 67, 72, 368
KasI GGCGCC 1 cut(s) 66
Kpn2I TCCGGA 2 cut(s) 24, 208
KpnI GGTACC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 5 cut(s) 21, 31, 49, 232, 292
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 4 cut(s) 201, 520, 535, 721
MaeI CTAG 3 cut(s) 170, 345, 719
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 23, 33, 51, 234, 294
MbiI CCGCTC 1 cut(s) 140
MboI GATC 5 cut(s) 21, 31, 49, 232, 292
MboII GAAGA 5 cut(s) 26, 252, 398, 473, 561
MflI RGATCY 2 cut(s) 21, 31
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 1 cut(s) 150
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 1 cut(s) 734
MnlI CCTC 7 cut(s) 21, 89, 128, 311, 341, 390, 547
MroI TCCGGA 2 cut(s) 24, 208
MseI TTAA 1 cut(s) 742
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 2 cut(s) 25, 209
MspR9I CCNGG 2 cut(s) 45, 146
MteI GCGCNGCGC 1 cut(s) 70
MvaI CCWGG 2 cut(s) 45, 146
MvnI CGCG 2 cut(s) 72, 370
MwoI GCNNNNNNNGC 2 cut(s) 486, 557
NarI GGCGCC 1 cut(s) 67
NdeII GATC 5 cut(s) 21, 31, 49, 232, 292
NlaIII CATG 3 cut(s) 66, 259, 631
NlaIV GGNNCC 2 cut(s) 68, 355
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 1 cut(s) 631
PaeR7I CTCGAG 1 cut(s) 132
PkrI GCNGC 6 cut(s) 71, 191, 535, 550, 553, 736
PleI GAGTC 1 cut(s) 733
PluTI GGCGCC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 733
Psp6I CCWGG 2 cut(s) 43, 144
PspGI CCWGG 2 cut(s) 43, 144
PspN4I GGNNCC 2 cut(s) 68, 355
PspPI GGNCC 1 cut(s) 142
PspXI VCTCGAGB 1 cut(s) 132
PstI CTGCAG 1 cut(s) 420
PstNI CAGNNNCTG 2 cut(s) 164, 734
PsuI RGATCY 2 cut(s) 21, 31
RsaI GTAC 2 cut(s) 355, 361
RsaNI GTAC 2 cut(s) 354, 360
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 1 cut(s) 742
SatI GCNGC 6 cut(s) 70, 190, 534, 549, 552, 735
Sau3AI GATC 5 cut(s) 21, 31, 49, 232, 292
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 45, 146
SduI GDGCHC 1 cut(s) 718
SetI ASST 8 cut(s) 81, 120, 194, 303, 328, 359, 550, 622
SfcI CTRYAG 2 cut(s) 416, 615
SfoI GGCGCC 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 132
SlaI CTCGAG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
Sse9I AATT 1 cut(s) 150
SsiI CCGC 7 cut(s) 70, 140, 175, 288, 370, 552, 575
SspDI GGCGCC 1 cut(s) 66
SspMI CTAG 3 cut(s) 170, 345, 719
StyD4I CCNGG 2 cut(s) 43, 144
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 4 cut(s) 166, 230, 401, 458
TaqI TCGA 4 cut(s) 14, 133, 239, 580
TasI AATT 1 cut(s) 150
TauI GCSGC 2 cut(s) 72, 554
Tru1I TTAA 1 cut(s) 742
Tru9I TTAA 1 cut(s) 742
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 4 cut(s) 189, 533, 548, 734
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 1 cut(s) 735
XceI RCATGY 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 132
XspI CTAG 3 cut(s) 170, 345, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.