RchiOBHm_Chr3g0487651

NC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003430
Physical Location & Seq
Forward (+)
34886587 .. 34890931
4345 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45208

Sequence Viewer

Length: 762 bp
ATGGGTTTGCTGTCAAACAAGATCCACAGGGACCAACTCAAACCTGCTGATCATATCTATACATGGAGGAACGCCTACCTCTACGCCCATCACGGGATATATGTCGGTGAGGGAAAGGTTATCCACTTCACTCGGGCAGCTGGGCAGGAAATTGGCACAGGAACTGTGTTAGACCGTTTCATTTTCAGCTCATCTCCATCCCATTCTTCAGAGAATCCCTGCACGAGTTGCGATGATCCATCAGGGCGTGATGGCGGTGTCATGTCTTCTTGCCTAGATTGTTTTCTGTCTGGTGGTGATCTGTACCGCTTTGAATATGGTGTCAATCCAGCTTTCTTTATTGCCAAAGCCAGAGGAGGTACCTGTACCCTTGCCTCGTCTGACCCATCTGAAGATGTCCTTCACCGTGCCTTTTTCCTTCTCCAGAACGGTTTTGGGAGCTACTGCCTTTTCAAAAACAATTGTGAAGACTTTGCAATTTATTGCAAAACAGGTTTGCTTGTGTTTACTAGCATTAGTGTGGGAAGGAGTGGGCAGGCAGCCTCCATTTTCTCTGCTGCAAGTGCTATTGCTTGTTCACCGGCTCGATATTTGACTACTACTTTTAGTGGTCTGAGTGCTTTCGGTGCTGGAATTTATTGTCTCCATCGATTGGTTTGTGATATTGGAATCCGCCGTGATGTTTCGAAAGTGCCAGTTGAGAGACTAGTTGATCGGGTTGGCTTCGTCGAGTCGGAGGTTGCAACTGAGATGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

253

Amino Acids

27.44

Weight (kDa)

6.69

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 167 6.8e-27 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 652
AciI CCGC 3 cut(s) 255, 307, 673
AclWI GGATC 2 cut(s) 16, 230
AcsI RAATTY 1 cut(s) 633
AcuI CTGAAG 2 cut(s) 192, 411
AfaI GTAC 3 cut(s) 305, 361, 367
AfiI CCNNNNNNNGG 2 cut(s) 93, 652
AgsI TTSAA 2 cut(s) 314, 454
AhlI ACTAGT 1 cut(s) 706
AluBI AGCT 4 cut(s) 140, 189, 332, 441
AluI AGCT 4 cut(s) 140, 189, 332, 441
Alw26I GTCTC 2 cut(s) 647, 697
AlwI GGATC 2 cut(s) 16, 230
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 137, 539, 557
ApoI RAATTY 1 cut(s) 633
Asp718I GGTACC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 119, 308, 395, 570
AsuII TTCGAA 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 359
BauI CACGAG 1 cut(s) 223
BbsI GAAGAC 2 cut(s) 258, 474
BbvI GCAGC 3 cut(s) 149, 544, 551
BccI CCATC 7 cut(s) 96, 205, 245, 247, 394, 654, 745
BceAI ACGGC 1 cut(s) 660
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 647, 697
BcuI ACTAGT 1 cut(s) 706
BfaI CTAG 3 cut(s) 275, 510, 707
BfuAI ACCTGC 1 cut(s) 52
BisI GCNGC 3 cut(s) 138, 540, 558
BlsI GCNGC 3 cut(s) 139, 541, 559
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 32, 361
BpiI GAAGAC 2 cut(s) 258, 474
BpmI CTGGAG 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 686
Bsa29I ATCGAT 1 cut(s) 649
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 652
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 1 cut(s) 695
BseCI ATCGAT 1 cut(s) 649
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 93, 652
BseMII CTCAG 2 cut(s) 605, 738
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 3 cut(s) 149, 544, 551
BseYI CCCAGC 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 205
BshNI GGYRCC 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 93, 652
BsmAI GTCTC 2 cut(s) 647, 697
BsmFI GGGAC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 686
Bsp143I GATC 5 cut(s) 21, 49, 235, 298, 712
BspACI CCGC 3 cut(s) 255, 307, 673
BspCNI CTCAG 2 cut(s) 606, 739
BspDI ATCGAT 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 32, 361
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 2 cut(s) 16, 230
BspT104I TTCGAA 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 580
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 580
BssMI GATC 5 cut(s) 21, 49, 235, 298, 712
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst4CI ACNGT 4 cut(s) 166, 176, 407, 431
BstAPI GCANNNNNTGC 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 2 cut(s) 614, 747
BstF5I GGATG 1 cut(s) 197
BstKTI GATC 5 cut(s) 24, 52, 238, 301, 715
BstMAI GTCTC 2 cut(s) 647, 697
BstMBI GATC 5 cut(s) 21, 49, 235, 298, 712
BstMWI GCNNNNNNNGC 3 cut(s) 228, 563, 626
BstV1I GCAGC 3 cut(s) 149, 544, 551
BstV2I GAAGAC 2 cut(s) 258, 474
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 649
BsuTUI ATCGAT 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 197
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 31
ClaI ATCGAT 1 cut(s) 649
Csp6I GTAC 3 cut(s) 304, 360, 366
CviAII CATG 2 cut(s) 63, 262
CviJI RGCY 8 cut(s) 140, 189, 332, 350, 441, 542, 584, 723
CviKI_1 RGCY 8 cut(s) 140, 189, 332, 350, 441, 542, 584, 723
CviQI GTAC 3 cut(s) 304, 360, 366
DdeI CTNAG 2 cut(s) 614, 747
DpnI GATC 5 cut(s) 23, 51, 237, 300, 714
DpnII GATC 5 cut(s) 21, 49, 235, 298, 712
EciI GGCGGA 1 cut(s) 662
Eco47I GGWCC 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 192, 411
Eco88I CYCGRG 1 cut(s) 132
FaeI CATG 2 cut(s) 66, 265
FaiI YATR 7 cut(s) 54, 60, 64, 100, 102, 263, 318
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 44
FatI CATG 2 cut(s) 62, 261
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 138, 540, 558
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 3 cut(s) 138, 540, 558
FspBI CTAG 3 cut(s) 275, 510, 707
GluI GCNGC 3 cut(s) 138, 540, 558
GsaI CCCAGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 407
HapII CCGG 1 cut(s) 581
Hin1II CATG 2 cut(s) 66, 265
HinfI GANTC 3 cut(s) 214, 669, 731
HpaII CCGG 1 cut(s) 581
HphI GGTGA 4 cut(s) 119, 308, 395, 570
Hpy166II GTNNAC 2 cut(s) 507, 578
Hpy188I TCNGA 5 cut(s) 211, 382, 391, 615, 736
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 2 cut(s) 507, 578
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 3 cut(s) 410, 428, 519
HpyCH4III ACNGT 4 cut(s) 166, 176, 407, 431
HpyCH4V TGCA 5 cut(s) 222, 476, 486, 560, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 563, 626
HpyF3I CTNAG 2 cut(s) 614, 747
Hsp92II CATG 2 cut(s) 66, 265
KpnI GGTACC 1 cut(s) 363
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 5 cut(s) 21, 49, 235, 298, 712
LmnI GCTCC 1 cut(s) 438
Lsp1109I GCAGC 3 cut(s) 149, 544, 551
MaeI CTAG 3 cut(s) 275, 510, 707
MalI GATC 5 cut(s) 23, 51, 237, 300, 714
MboI GATC 5 cut(s) 21, 49, 235, 298, 712
MboII GAAGA 4 cut(s) 198, 258, 404, 479
MfeI CAATTG 1 cut(s) 460
MflI RGATCY 1 cut(s) 21
MluCI AATT 4 cut(s) 150, 460, 477, 633
MlyI GAGTC 1 cut(s) 740
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 8 cut(s) 60, 89, 103, 347, 350, 385, 553, 730
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 1 cut(s) 581
MunI CAATTG 1 cut(s) 460
MwoI GCNNNNNNNGC 3 cut(s) 228, 563, 626
NdeII GATC 5 cut(s) 21, 49, 235, 298, 712
NlaIII CATG 2 cut(s) 66, 265
NlaIV GGNNCC 2 cut(s) 32, 361
NspV TTCGAA 1 cut(s) 686
PfeI GAWTC 2 cut(s) 214, 669
PflMI CCANNNNNTGG 1 cut(s) 652
PkrI GCNGC 3 cut(s) 139, 541, 559
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 32, 361
PspPI GGNCC 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 3 cut(s) 305, 361, 367
RsaNI GTAC 3 cut(s) 304, 360, 366
RseI CAYNNNNRTG 1 cut(s) 518
SatI GCNGC 3 cut(s) 138, 540, 558
Sau3AI GATC 5 cut(s) 21, 49, 235, 298, 712
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 1 cut(s) 740
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 706
Sse9I AATT 4 cut(s) 150, 460, 477, 633
SsiI CCGC 3 cut(s) 255, 307, 673
SspMI CTAG 3 cut(s) 275, 510, 707
TaaI ACNGT 4 cut(s) 166, 176, 407, 431
TaqI TCGA 4 cut(s) 586, 649, 686, 729
TasI AATT 4 cut(s) 150, 460, 477, 633
TfiI GAWTC 2 cut(s) 214, 669
TseI GCWGC 3 cut(s) 137, 539, 557
TspDTI ATGAA 1 cut(s) 169
Van91I CCANNNNNTGG 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 633
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XspI CTAG 3 cut(s) 275, 510, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.