Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002914

Physical Location & Seq

Genomic Coordinates Reverse (-)

9718882 .. 9724589

Gene Structure 5708 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1070100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGTCTGATGGTGAAGGATCCGATTCGGAGGATTATCCGTCAATCAGAGACTCGGACTCAGAAGGCGAAGAGAATGATTTCTCCTCCGGAGATGAGGATGTTATCAACACAAGTGACAATGATGGTGTGGCCGGTGATTATGGTAACAGTGATGATGAAAGTGATAGTTCTGAACTTCATGAGGGGGTTGAGGAGAGTGATTCATCTGAGGATGAGGTGGTTCCTCGAAACACAGTCGGTGATGTTCCCTTGGAGTGGTATCGGGATGAGAAACATATTGGTTATGACATTAAAGGGAAGAAGATAAAGAAGAAGGAAAAAGCAGATAAATTGCAATCCTTTCTTGCTAGTGCTGATGATTCAAAGAACTGGCGCAAAATTTATGATGAATATAATGATGAGGAAGTAGAACTGACAAAAGAAGATATCAAATCGATTAGTAGACTGCTCGAAGGAAAGGCTCCACATGGTGACTTTGATCCATATGCGCCATATGTTGATTGGTTTAAATGGGATGACTCCAACCATCCACTGTCAAATGCACCTGAACCCAAGAGACGATTCATTCCTTCAAAATCTGAAAGTAAACTGGTTAATAAGATTAAGTTGGCCATTCGTAAGGGTTTAATTAAGCCTAAAAAGTCCAAAGAGGAAGAAGAAGAAGAAGAAGAAATTTATCCACTGTGGGAAGATGACTCTAATACAATGGAAAAGGATAACCATCTATCTTACATTCCTGCACCAAAGCCCAAATTGCCTGGCCATGAGGAGTCCTTCAATCCATCTTTAGAATACATCCCAACACAAGAAGAGATCAACTCTTACCAGTTGATGTATGAAGAAGACCGTCCTAAATTTATACCTAAAAGGTTTGCAAATATGAGAAGCATCCCTGCATATGAGAATGCTGTGAAGGAATGCTTTGAGAGATGTTTGGATCTATATTTGTGCCCCAGAGTTCGAAAAAAACGTCTTAATATCGATCCTGAATCTCTGAAGCCCAAGCTACCGAGCCGAACTGAACTTAAGCCTTACCCTGTAGCATGTTATCTTGAGTATAGAGGTCACGACGATGCAGTTACATCTGTTTCTGTAGAAGCCTCAGGGCAGTGGATTGCATCAGGTTCTTTGGATGGAACTATGCGTATTTGGGAGGTCGAAACCGGTAGATGTGTCAAGATCTGCGAGATTGGTGAAGCCGTCAAATATGTATCTTGGAATCCTAATCCTGAGCATTCTATAGTGGCTGTCTCGGCGGGACAAGACGTACTTCTTTTGAACACTGGTTGTGGGGATGAAGAAGTACAGAAAAGAACCAAAGAACTTCTCTCTGTTGAAATGCCTATGCCTGATGACTCTGGCAAAACAGCATCTAGTGTAAGCTGGCTTCATGATGATAAACTTGAGGGGGTCAGATTAAGGCATTCCAAGACCGTGGCTTCGGTAGAATGGCATCGTAAAGGAGACTACCTTTCGACACTCTCAAAGAAGTTTACTCAGACACTTTCATTCAAGTTGCATGGGATTGCAGTTACTTCAGTTTTCCATCCTACACGGTCTTTCTTCTTCATTTCAACAAAGAAGACTGTTCGTGTTTATGATCTGGTGAAGGATGGAAAACTTGTCAAAAAGCTTGAGACTGGACTTCGTGAAGTCTCATCTATTGCAGTCCATCCTTCTGGTGATCATATAATTGTGGGAAGCAGAGAAGGAAAATTATGTTGGTTCGACATGGACCTTTCATCTAAACCTTACAAAACTCTCAAGTTGCATCAGAAGGACATCAACAATGTATCTTTCCATCGTTCGTACCCGCTGTTTGCAACATGCTCTGATGACTGCACTGCATATGTTTTTCATGGAATGGTTTATTCAGATCTTAACCAGAACCCTCTTATCGTTCCATTGGAAATACTCAGAGGTCATACAAGCTCAAATGGGAGAGGTGTATTGGACTGTAAGTTCCATCCAAGGCAGCCATGGTTATTCACAAGCGGCGCAGACTCGGTGATTAGACTTTATTGCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030684 GO:0030686 GO:0030687 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044877 GO:0046483 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BOP1NT WD40

Protein Analysis

677

Amino Acids

76.87

Weight (kDa)

5.25

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BOP1NT	PF08145	87 - 346	2e-99	BOP1NT (NUC169) domain
WD40	PF00400	349 - 384	4.7e-09	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	351 - 429	1.2e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	352 - 408	2e-07	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	352 - 416	4e-06	Prp19 WD40 domain
EIF3I	PF24805	353 - 417	3.7e-09	EIF3I
Beta-prop_WDR5	PF25175	353 - 427	1.6e-11	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	353 - 471	8e-08	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	356 - 425	3.9e-10	THOC3 beta-propeller domain
Beta-prop_THOC3	PF25174	475 - 674	3.2e-14	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	479 - 619	2e-09	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	491 - 674	3.7e-07	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	496 - 645	5.8e-08	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	503 - 579	5.9e-06	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	516 - 620	1.1e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	523 - 674	2.9e-11	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	525 - 674	1.4e-06	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	550 - 674	4.9e-10	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40360 AT2G40360
fragaria_vesca	FvH4_5g21650 FvH4_5g21650 FvH4_5g21650
malus_domestica	MD05G1062400.v1.1 MD10G1070100.v1.1
prunus_persica	Prupe.6G114000_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1
pyrus_communis	pycom05g05370 pycom05g05390 pycom10g05570
rosa_chinensis	RchiOBHm_Chr7g0209131 RchiOBHm_Chr7g0241521 RchiOBHm_Chr7g0241531 RchiOBHm_Chr7g0241541
rosa_laevigata	RLG00000000568
rosa_multiflora	Rmu_sc0000821.1_g000025 Rmu_sc0002268.1_g000023
rosa_roxburghii	Rroxscaffold_3G00218950 Rroxscaffold_3G00249700
rosa_rugosa	Rorug07G0109700 Rorug07G0109800 Rorug07G0345000
rosa_samantha	Rh7AG243600 Rh7AG499500 Rh7AG499600 Rh7BG238700 Rh7BG470600 Rh7BG470700 Rh7CG259100 Rh7DG250600 Rh7DG484000 Rh7DG484200
rosa_wichuraiana	Rw7G020660 Rw7G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	442
AccIII	TCCGGA	1 cut(s)	86
AciI	CCGC	3 cut(s)	1256, 1814, 1995
AclWI	GGATC	5 cut(s)	12, 25, 473, 945, 977
AcoI	YGGCCR	3 cut(s)	129, 609, 760
AcsI	RAATTY	3 cut(s)	378, 672, 854
AcuI	CTGAAG	2 cut(s)	1016, 1521
AdeI	CACNNNGTG	1 cut(s)	470
AfaI	GTAC	3 cut(s)	1269, 1305, 1811
AfiI	CCNNNNNNNGG	1 cut(s)	255
AflII	CTTAAG	1 cut(s)	1025
AgeI	ACCGGT	1 cut(s)	1163
AgsI	TTSAA	7 cut(s)	363, 573, 778, 1279, 1337, 1513, 1575
AjnI	CCWGG	1 cut(s)	757
AjuI	GAANNNNNNNTTGG	4 cut(s)	545, 577, 1705, 1737
AloI	GAACNNNNNNTCC	2 cut(s)	1946, 1978
AluBI	AGCT	4 cut(s)	1006, 1383, 1633, 1932
AluI	AGCT	4 cut(s)	1006, 1383, 1633, 1932
Alw26I	GTCTC	6 cut(s)	42, 550, 1255, 1458, 1631, 1660
AlwI	GGATC	5 cut(s)	12, 25, 473, 945, 977
Aor13HI	TCCGGA	1 cut(s)	86
AoxI	GGCC	3 cut(s)	129, 609, 760
ApeKI	GCWGC	1 cut(s)	1975
ApoI	RAATTY	3 cut(s)	378, 672, 854
ArsI	GACNNNNNNTTYG	4 cut(s)	955, 987, 1423, 1455
AsiGI	ACCGGT	1 cut(s)	1163
Asp700I	GAANNNNTTC	1 cut(s)	77
AspLEI	GCGC	3 cut(s)	375, 490, 2000
AspS9I	GGNCC	1 cut(s)	1735
AsuHPI	GGTGA	8 cut(s)	23, 146, 251, 482, 1205, 1618, 1694, 2020
AsuII	TTCGAA	1 cut(s)	961
AvaII	GGWCC	1 cut(s)	1735
AxyI	CCTNAGG	1 cut(s)	1102
BaeGI	GKGCMC	1 cut(s)	953
BalI	TGGCCA	2 cut(s)	611, 762
BamHI	GGATCC	1 cut(s)	17
BarI	GAAGNNNNNNTAC	2 cut(s)	1371, 1403
BbsI	GAAGAC	2 cut(s)	849, 1589
BbvI	GCAGC	1 cut(s)	1987
BceAI	ACGGC	1 cut(s)	1184
BciT130I	CCWGG	1 cut(s)	759
BclI	TGATCA	1 cut(s)	1684
BcoDI	GTCTC	6 cut(s)	42, 550, 1255, 1458, 1631, 1660
BfaI	CTAG	2 cut(s)	348, 1374
BfmI	CTRYAG	3 cut(s)	1038, 1092, 1239
BfrI	CTTAAG	1 cut(s)	1025
BglII	AGATCT	2 cut(s)	1179, 1876
BisI	GCNGC	2 cut(s)	1976, 1996
BlsI	GCNGC	2 cut(s)	1977, 1997
Bme1390I	CCNGG	1 cut(s)	759
Bme18I	GGWCC	1 cut(s)	1735
BmgT120I	GGNCC	1 cut(s)	1735
BmiI	GGNNCC	3 cut(s)	19, 222, 462
BmrFI	CCNGG	1 cut(s)	759
BmsI	GCATC	6 cut(s)	897, 1063, 1127, 1379, 1462, 1780
BpiI	GAAGAC	2 cut(s)	849, 1589
BplI	GAGNNNNNCTC	2 cut(s)	803, 835
Bpu10I	CCTNAGC	1 cut(s)	1230
Bpu14I	TTCGAA	1 cut(s)	961
BpuEI	CTTGAG	4 cut(s)	1073, 1424, 1655, 1748
Bsa29I	ATCGAT	2 cut(s)	434, 981
BsaBI	GATNNNNATC	1 cut(s)	720
BsaJI	CCNNGG	4 cut(s)	249, 1434, 1970, 1979
BsaWI	WCCGGW	2 cut(s)	86, 1163
Bsc4I	CCNNNNNNNGG	1 cut(s)	255
Bse118I	RCCGGY	2 cut(s)	131, 1163
Bse1I	ACTGG	5 cut(s)	374, 594, 826, 1288, 1645
Bse21I	CCTNAGG	1 cut(s)	1102
Bse8I	GATNNNNATC	1 cut(s)	720
BseAI	TCCGGA	1 cut(s)	86
BseBI	CCWGG	1 cut(s)	759
BseCI	ATCGAT	2 cut(s)	434, 981
BseDI	CCNNGG	4 cut(s)	249, 1434, 1970, 1979
BseJI	GATNNNNATC	1 cut(s)	720
BseLI	CCNNNNNNNGG	1 cut(s)	255
BseMII	CTCAG	6 cut(s)	72, 198, 1116, 1221, 1511, 1930
BseNI	ACTGG	5 cut(s)	374, 594, 826, 1288, 1645
BseRI	GAGGAG	3 cut(s)	73, 206, 782
BseSI	GKGCMC	1 cut(s)	953
BseXI	GCAGC	1 cut(s)	1987
BsgI	GTGCAG	2 cut(s)	723, 1825
BshFI	GGCC	3 cut(s)	131, 611, 762
BshTI	ACCGGT	1 cut(s)	1163
BshVI	ATCGAT	2 cut(s)	434, 981
BsiSI	CCGG	3 cut(s)	87, 132, 1164
BslFI	GGGAC	1 cut(s)	1272
BslI	CCNNNNNNNGG	1 cut(s)	255
BsmAI	GTCTC	6 cut(s)	42, 550, 1255, 1458, 1631, 1660
BsmBI	CGTCTC	1 cut(s)	550
BsmFI	GGGAC	1 cut(s)	1272
BsmI	GAATGC	4 cut(s)	910, 923, 1234, 1423
BsnI	GGCC	3 cut(s)	131, 611, 762
Bsp119I	TTCGAA	1 cut(s)	961
Bsp1286I	GDGCHC	1 cut(s)	953
Bsp13I	TCCGGA	1 cut(s)	86
Bsp143I	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
Bsp19I	CCATGG	1 cut(s)	1979
BspACI	CCGC	3 cut(s)	1256, 1814, 1995
BspANI	GGCC	3 cut(s)	131, 611, 762
BspCNI	CTCAG	6 cut(s)	71, 199, 1115, 1222, 1510, 1929
BspDI	ATCGAT	2 cut(s)	434, 981
BspEI	TCCGGA	1 cut(s)	86
BspHI	TCATGA	2 cut(s)	178, 1390
BspLI	GGNNCC	3 cut(s)	19, 222, 462
BspPI	GGATC	5 cut(s)	12, 25, 473, 945, 977
BspT104I	TTCGAA	1 cut(s)	961
BspTI	CTTAAG	1 cut(s)	1025
BsrFI	RCCGGY	2 cut(s)	131, 1163
BsrI	ACTGG	5 cut(s)	374, 594, 826, 1288, 1645
BssAI	RCCGGY	2 cut(s)	131, 1163
BssECI	CCNNGG	4 cut(s)	249, 1434, 1970, 1979
BssMI	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
BssT1I	CCWWGG	3 cut(s)	249, 1970, 1979
Bst2UI	CCWGG	1 cut(s)	759
Bst4CI	ACNGT	9 cut(s)	149, 235, 534, 684, 848, 1435, 1557, 1588, 1958
Bst6I	CTCTTC	2 cut(s)	63, 804
BstAFI	CTTAAG	1 cut(s)	1025
BstBI	TTCGAA	1 cut(s)	961
BstC8I	GCNNGC	1 cut(s)	1385
BstDEI	CTNAG	6 cut(s)	58, 207, 1102, 1230, 1497, 1916
BstDSI	CCRYGG	2 cut(s)	1434, 1979
BstHHI	GCGC	3 cut(s)	375, 490, 2000
BstKTI	GATC	9 cut(s)	20, 481, 816, 940, 985, 1182, 1603, 1687, 1879
BstMAI	GTCTC	6 cut(s)	42, 550, 1255, 1458, 1631, 1660
BstMBI	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
BstMWI	GCNNNNNNNGC	2 cut(s)	754, 1253
BstNI	CCWGG	1 cut(s)	759
BstNSI	RCATGY	2 cut(s)	1047, 1830
BstSCI	CCNGG	1 cut(s)	757
BstSFI	CTRYAG	3 cut(s)	1038, 1092, 1239
BstSLI	GKGCMC	1 cut(s)	953
BstV1I	GCAGC	1 cut(s)	1987
BstV2I	GAAGAC	2 cut(s)	849, 1589
BstX2I	RGATCY	4 cut(s)	17, 937, 1179, 1876
BstXI	CCANNNNNNTGG	2 cut(s)	1435, 1679
BstYI	RGATCY	4 cut(s)	17, 937, 1179, 1876
Bsu15I	ATCGAT	2 cut(s)	434, 981
Bsu36I	CCTNAGG	1 cut(s)	1102
BsuRI	GGCC	3 cut(s)	131, 611, 762
BsuTUI	ATCGAT	2 cut(s)	434, 981
BtgI	CCRYGG	2 cut(s)	1434, 1979
BtsI	GCAGTG	2 cut(s)	1115, 1842
BtsIMutI	CAGTG	6 cut(s)	154, 530, 680, 1115, 1281, 1842
Cac8I	GCNNGC	1 cut(s)	1385
CciI	TCATGA	2 cut(s)	178, 1390
CfoI	GCGC	3 cut(s)	375, 490, 2000
Cfr10I	RCCGGY	2 cut(s)	131, 1163
Cfr13I	GGNCC	1 cut(s)	1735
ClaI	ATCGAT	2 cut(s)	434, 981
Csp6I	GTAC	3 cut(s)	1268, 1304, 1810
CspAI	ACCGGT	1 cut(s)	1163
CviQI	GTAC	3 cut(s)	1268, 1304, 1810
DdeI	CTNAG	6 cut(s)	58, 207, 1102, 1230, 1497, 1916
DpnI	GATC	9 cut(s)	19, 480, 815, 939, 984, 1181, 1602, 1686, 1878
DpnII	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
DraI	TTTAAA	1 cut(s)	508
DraIII	CACNNNGTG	1 cut(s)	470
EaeI	YGGCCR	3 cut(s)	129, 609, 760
Eam1104I	CTCTTC	2 cut(s)	63, 804
EarI	CTCTTC	2 cut(s)	63, 804
Eco130I	CCWWGG	3 cut(s)	249, 1970, 1979
Eco32I	GATATC	1 cut(s)	427
Eco47I	GGWCC	1 cut(s)	1735
Eco57I	CTGAAG	2 cut(s)	1016, 1521
Eco81I	CCTNAGG	1 cut(s)	1102
EcoRII	CCWGG	1 cut(s)	757
EcoRV	GATATC	1 cut(s)	427
EcoT14I	CCWWGG	3 cut(s)	249, 1970, 1979
ErhI	CCWWGG	3 cut(s)	249, 1970, 1979
Esp3I	CGTCTC	1 cut(s)	550
FalI	AAGNNNNNCTT	4 cut(s)	905, 937, 1254, 1286
FaqI	GGGAC	1 cut(s)	1272
FauI	CCCGC	2 cut(s)	1249, 1821
FauNDI	CATATG	4 cut(s)	484, 493, 898, 1849
FbaI	TGATCA	1 cut(s)	1684
FblI	GTMKAC	1 cut(s)	442
Fnu4HI	GCNGC	2 cut(s)	1976, 1996
Fsp4HI	GCNGC	2 cut(s)	1976, 1996
FspBI	CTAG	2 cut(s)	348, 1374
GlaI	GCGC	3 cut(s)	374, 489, 1999
GluI	GCNGC	2 cut(s)	1976, 1996
HaeIII	GGCC	3 cut(s)	131, 611, 762
HapII	CCGG	3 cut(s)	87, 132, 1164
HhaI	GCGC	3 cut(s)	375, 490, 2000
Hin6I	GCGC	3 cut(s)	373, 488, 1998
HinP1I	GCGC	3 cut(s)	373, 488, 1998
HindIII	AAGCTT	1 cut(s)	1631
HpaII	CCGG	3 cut(s)	87, 132, 1164
HphI	GGTGA	8 cut(s)	23, 146, 251, 482, 1205, 1618, 1694, 2020
Hpy166II	GTNNAC	3 cut(s)	443, 587, 1494
Hpy8I	GTNNAC	3 cut(s)	443, 587, 1494
Hpy99I	CGWCG	1 cut(s)	1073
HpyCH4III	ACNGT	9 cut(s)	149, 235, 534, 684, 848, 1435, 1557, 1588, 1958
HpyCH4IV	ACGT	2 cut(s)	970, 1266
HpyF10VI	GCNNNNNNNGC	2 cut(s)	754, 1253
HpyF3I	CTNAG	6 cut(s)	58, 207, 1102, 1230, 1497, 1916
HpySE526I	ACGT	2 cut(s)	970, 1266
HspAI	GCGC	3 cut(s)	373, 488, 1998
Kpn2I	TCCGGA	1 cut(s)	86
Ksp22I	TGATCA	1 cut(s)	1684
Kzo9I	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
LmnI	GCTCC	1 cut(s)	466
Lsp1109I	GCAGC	1 cut(s)	1987
LweI	GCATC	6 cut(s)	897, 1063, 1127, 1379, 1462, 1780
MaeI	CTAG	2 cut(s)	348, 1374
MaeII	ACGT	2 cut(s)	970, 1266
MaeIII	GTNAC	6 cut(s)	113, 143, 470, 1064, 1078, 1531
MalI	GATC	9 cut(s)	19, 480, 815, 939, 984, 1181, 1602, 1686, 1878
MboI	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
MfeI	CAATTG	1 cut(s)	2026
MflI	RGATCY	4 cut(s)	17, 937, 1179, 1876
MhlI	GDGCHC	1 cut(s)	953
MlsI	TGGCCA	2 cut(s)	611, 762
MluCI	AATT	9 cut(s)	329, 378, 627, 672, 752, 854, 1692, 1715, 2026
MluNI	TGGCCA	2 cut(s)	611, 762
MlyI	GAGTC	7 cut(s)	44, 50, 512, 689, 779, 1349, 1997
MmeI	TCCRAC	1 cut(s)	546
Mox20I	TGGCCA	2 cut(s)	611, 762
MroI	TCCGGA	1 cut(s)	86
MroXI	GAANNNNTTC	1 cut(s)	77
MscI	TGGCCA	2 cut(s)	611, 762
MslI	CAYNNNNRTG	1 cut(s)	1071
Msp20I	TGGCCA	2 cut(s)	611, 762
MspA1I	CMGCKG	1 cut(s)	1816
MspCI	CTTAAG	1 cut(s)	1025
MspI	CCGG	3 cut(s)	87, 132, 1164
MspR9I	CCNGG	1 cut(s)	759
MunI	CAATTG	1 cut(s)	2026
Mva1269I	GAATGC	4 cut(s)	910, 923, 1234, 1423
MvaI	CCWGG	1 cut(s)	759
MwoI	GCNNNNNNNGC	2 cut(s)	754, 1253
NcoI	CCATGG	1 cut(s)	1979
NdeI	CATATG	4 cut(s)	484, 493, 898, 1849
NdeII	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
NlaIV	GGNNCC	3 cut(s)	19, 222, 462
NmeAIII	GCCGAG	1 cut(s)	1232
NmuCI	GTSAC	3 cut(s)	113, 470, 1064
NspI	RCATGY	2 cut(s)	1047, 1830
NspV	TTCGAA	1 cut(s)	961
PacI	TTAATTAA	1 cut(s)	630
PagI	TCATGA	2 cut(s)	178, 1390
PcsI	WCGNNNNNNNCGW	1 cut(s)	967
PctI	GAATGC	4 cut(s)	910, 923, 1234, 1423
PdmI	GAANNNNTTC	1 cut(s)	77
PfeI	GAWTC	6 cut(s)	23, 200, 359, 561, 989, 1219
PinAI	ACCGGT	1 cut(s)	1163
PkrI	GCNGC	2 cut(s)	1977, 1997
PleI	GAGTC	7 cut(s)	44, 50, 512, 689, 778, 1349, 1997
PpsI	GAGTC	7 cut(s)	44, 50, 512, 689, 778, 1349, 1997
Psp6I	CCWGG	1 cut(s)	757
PspGI	CCWGG	1 cut(s)	757
PspN4I	GGNNCC	3 cut(s)	19, 222, 462
PspPI	GGNCC	1 cut(s)	1735
PsuI	RGATCY	4 cut(s)	17, 937, 1179, 1876
RsaI	GTAC	3 cut(s)	1269, 1305, 1811
RsaNI	GTAC	3 cut(s)	1268, 1304, 1810
RseI	CAYNNNNRTG	1 cut(s)	1071
SatI	GCNGC	2 cut(s)	1976, 1996
Sau3AI	GATC	9 cut(s)	17, 478, 813, 937, 982, 1179, 1600, 1684, 1876
Sau96I	GGNCC	1 cut(s)	1735
SchI	GAGTC	7 cut(s)	44, 50, 512, 689, 779, 1349, 1997
ScrFI	CCNGG	1 cut(s)	759
SduI	GDGCHC	1 cut(s)	953
SfaNI	GCATC	6 cut(s)	897, 1063, 1127, 1379, 1462, 1780
SfcI	CTRYAG	3 cut(s)	1038, 1092, 1239
SfuI	TTCGAA	1 cut(s)	961
SinI	GGWCC	1 cut(s)	1735
SmiMI	CAYNNNNRTG	1 cut(s)	1071
SmlI	CTYRAG	5 cut(s)	1025, 1052, 1403, 1634, 1763
SmoI	CTYRAG	5 cut(s)	1025, 1052, 1403, 1634, 1763
Sse9I	AATT	9 cut(s)	329, 378, 627, 672, 752, 854, 1692, 1715, 2026
SsiI	CCGC	3 cut(s)	1256, 1814, 1995
SspMI	CTAG	2 cut(s)	348, 1374
StyD4I	CCNGG	1 cut(s)	757
StyI	CCWWGG	3 cut(s)	249, 1970, 1979
TaaI	ACNGT	9 cut(s)	149, 235, 534, 684, 848, 1435, 1557, 1588, 1958
TaiI	ACGT	2 cut(s)	973, 1269
TaqI	TCGA	8 cut(s)	226, 434, 450, 961, 981, 1158, 1475, 1728
TasI	AATT	9 cut(s)	329, 378, 627, 672, 752, 854, 1692, 1715, 2026
TatI	WGTACW	1 cut(s)	1303
TauI	GCSGC	1 cut(s)	1998
TfiI	GAWTC	6 cut(s)	23, 200, 359, 561, 989, 1219
TscAI	CASTG	6 cut(s)	154, 537, 687, 1115, 1288, 1849
TseFI	GTSAC	3 cut(s)	113, 470, 1064
TseI	GCWGC	1 cut(s)	1975
Tsp45I	GTSAC	3 cut(s)	113, 470, 1064
TspGWI	ACGGA	1 cut(s)	27
TspRI	CASTG	6 cut(s)	154, 537, 687, 1115, 1288, 1849
Vha464I	CTTAAG	1 cut(s)	1025
VpaK11BI	GGWCC	1 cut(s)	1735
XapI	RAATTY	3 cut(s)	378, 672, 854
XceI	RCATGY	2 cut(s)	1047, 1830
XcmI	CCANNNNNNNNNTGG	2 cut(s)	498, 1977
XmiI	GTMKAC	1 cut(s)	442
XmnI	GAANNNNTTC	1 cut(s)	77
XspI	CTAG	2 cut(s)	348, 1374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.