Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0002914

Physical Location & Seq

Genomic Coordinates Forward (+)

25250681 .. 25256996

Gene Structure 6316 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG259100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGAAGGTGAAGAAGAAGAGCTCTGTTGCGGCAGACTCGGTGAAAGAACAACAGAAGAAGCAAAAAGGGAAGGGGAAGATGAAGATGAAGATTCCTGAAGCGAAACAAGAAAAACAGCAACCGGAGGAAGTAGACTCTGATTCTGTTTACGATTCCGGAGCTGAAGATGAGAGGAGTCTGTCAAATCATGAGGATATGTCTGGTGGTGAAGGATCTGACTTAGAGGATTATCCATCAATCAGAGACACAGACTCGGAAGATGAAGAGAGTGGTTTTTCTGAAGATGAGGGTGTGCTCAACACCAGCAGAAATGATGGTTTGGCCATTGATCATGGTAATGAGGAGGAAAGTGATAGTTCTGGACTTCATGAAGTAGTCAAGGAAAGTGATTCATCTGAAGATGAGGTTGCTCCTCGAAATACAATTGGTGCTGTTCCCTTGGAGTGGTATCGGGATGAGAAACATATTGGTTATGATCTTACAGGGAAGAAGATTAAAAAGAAGGAAAGAGAAGATAAATTACAATCCTTTCTTGCTAGCGCTGATGATTCCAAAAATTGGCGCAAATTTTTGGATGAATACAATGACGAGGAAGTAGAAGTGACAAAAGAAGATATGAAAACTATAAATAGATTGCTTGAAGGAAAGGCGCCACACGGTGACTTTGATCCATATGCGCCTTATGTTGATTGGTTTAAATGGGATGACTCAAAGCATCCACTGTCAAATGCTCCTGAACCCAAGAGACGATTCATTCCTTCAAAATCTGAAAGTAAACTGGTGAAGAGGTTAGTATTAGCAATTCGGAAGGGTTTAATTAAGCCTAGAAAGGATGAAGAAGAAGAAGAAGAAGAAGAAAGTGTTTATCCCTTGTGGGGAGACGACTCTAATTCAACGGAAAAGGATGGTAGCCATTTATCTTACATTCCAGCACCAAAGCCAAAATTGCCAGGGTATGAGGAGTCCTTCAATCCTCCTCCAGAATTCATCCCATCACAAGAAGAGATTGATGCTTATCAGTTAATGTATGAGGAAGACCGCCCTAAGTTTATACCAAAAAGGTTTACATCTATGAGAAGCATCCCTGCATATGAGAATGCTATTAAGGAGTGCTTCGAAAGATGTTTGGATCTATACTTGTGCCCCAGAGTTCGGAAGAAACGTATCAATATTGATCCTGAATCTCTGAAGCCCAAGCTACCAAGCCGAAAGGATCTCAAGCCTTATCCTGTGAAATGTTATCTAGAGTATAGAGGTCACAAAGACACAGTTACATCAGTTTCCACAGAAGTTTCAGGGCAGTGGATTGCATCAGGTTCATTGGATGGAACTGTGCGAATTTGGGAGGTTGAAACTGGTAGATGTCTCAAACTCTGGGAGATTGGAGAAGCTGTCACATATGTAGCTTGGAATCCCAATCCTGAGTATTCCGTATTGGCTGTCTCTGTGGGACAAGATGTACTTATTCTCAACACTGGTTTTGGGAATGAAGAAGTACAGAAAAAAACTAAAGAACTTCTTTCGGCTGAAACACCTACCACACCAGATGACACGGCTACCTTCGTGAGCTGGATTCAAGATGAAACACATGAGGGCACCAGGTTAAGACATTTTAAGACTGTGTCTTCAGTGGAATGGCATCGTAGAGGAGACTACTTTTCAACGGTGGTGCCAGCAGGCCAATCAAAATCAATTTTTATGCATAGGCTCTCTCAGAAGTCTACCCAGACATTTTCAATTAAGTTGGGTGGGCTTGCAGTTACTTCAGTTTTCCATCCTGTTCGCTCCCACATCTTCATTTCTACGAAGAAAACTATTCGCCAATATGATTTGGTGAAGGGAAAGCAAATCAGAAAGCTTGATACAGGACTAAAAGAAATCTCCTCCATTGCAGTTCATCCTTCTGGTGATCATGTAATTGTGGGGAGCAGAGAAGGAAAATTTTGTTGGTTTGATATGGACCTTTCAACCAAAAAACCTTACCTTATTCGCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030684 GO:0030686 GO:0030687 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044877 GO:0046483 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BOP1NT WD40

Protein Analysis

665

Amino Acids

75.85

Weight (kDa)

5.48

Isoelectric Point (pI)

49.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BOP1NT	PF08145	150 - 410	1.1e-99	BOP1NT (NUC169) domain
WD40	PF00400	412 - 448	1.9e-09	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	413 - 492	7.6e-13	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	415 - 550	4.4e-11	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	415 - 498	2.8e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	415 - 488	2.2e-07	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	416 - 477	1.3e-06	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	417 - 494	1.9e-08	EIF3I
Beta-prop_CAF1B_HIR1	PF24105	417 - 492	7.4e-06	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	525 - 654	7.2e-07	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	537 - 654	1.6e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40360 AT2G40360
fragaria_vesca	FvH4_5g21650 FvH4_5g21650 FvH4_5g21650
malus_domestica	MD05G1062400.v1.1 MD10G1070100.v1.1
prunus_persica	Prupe.6G114000_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1
pyrus_communis	pycom05g05370 pycom05g05390 pycom10g05570
rosa_chinensis	RchiOBHm_Chr7g0209131 RchiOBHm_Chr7g0241521 RchiOBHm_Chr7g0241531 RchiOBHm_Chr7g0241541
rosa_laevigata	RLG00000000568
rosa_multiflora	Rmu_sc0000821.1_g000025 Rmu_sc0002268.1_g000023
rosa_roxburghii	Rroxscaffold_3G00218950 Rroxscaffold_3G00249700
rosa_rugosa	Rorug07G0109700 Rorug07G0109800 Rorug07G0345000
rosa_samantha	Rh7AG243600 Rh7AG499500 Rh7AG499600 Rh7BG238700 Rh7BG470600 Rh7BG470700 Rh7CG259100 Rh7DG250600 Rh7DG484000 Rh7DG484200
rosa_wichuraiana	Rw7G020660 Rw7G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	649, 1595, 1669
AccB7I	CCANNNNNTGG	1 cut(s)	558
AccI	GTMKAC	2 cut(s)	132, 1721
AccIII	TCCGGA	1 cut(s)	155
AciI	CCGC	2 cut(s)	29, 1039
AclWI	GGATC	5 cut(s)	220, 662, 1137, 1169, 1221
AcoI	YGGCCR	1 cut(s)	321
AcsI	RAATTY	4 cut(s)	566, 983, 1338, 1940
AcuI	CTGAAG	7 cut(s)	117, 183, 300, 417, 1208, 1611, 1749
AcyI	GRCGYC	1 cut(s)	650
AdeI	CACNNNGTG	1 cut(s)	659
AfaI	GTAC	2 cut(s)	1461, 1497
AfeI	AGCGCT	1 cut(s)	541
AfiI	CCNNNNNNNGG	4 cut(s)	444, 558, 875, 1152
AgsI	TTSAA	9 cut(s)	641, 762, 894, 970, 1352, 1577, 1662, 1737, 1968
AjnI	CCWGG	2 cut(s)	949, 1598
AjuI	GAANNNNNNNTTGG	4 cut(s)	734, 766, 1930, 1962
AluBI	AGCT	7 cut(s)	21, 161, 1198, 1391, 1406, 1569, 1858
AluI	AGCT	7 cut(s)	21, 161, 1198, 1391, 1406, 1569, 1858
Alw21I	GWGCWC	2 cut(s)	23, 297
Alw26I	GTCTC	6 cut(s)	237, 739, 873, 1370, 1447, 1644
AlwI	GGATC	5 cut(s)	220, 662, 1137, 1169, 1221
Aor13HI	TCCGGA	1 cut(s)	155
Aor51HI	AGCGCT	1 cut(s)	541
AoxI	GGCC	2 cut(s)	321, 1678
ApoI	RAATTY	4 cut(s)	566, 983, 1338, 1940
AspLEI	GCGC	4 cut(s)	542, 564, 652, 679
AspS9I	GGNCC	1 cut(s)	1960
AsuHPI	GGTGA	7 cut(s)	19, 52, 218, 671, 793, 1846, 1919
AsuII	TTCGAA	1 cut(s)	1116
AsuNHI	GCTAGC	1 cut(s)	536
AvaII	GGWCC	1 cut(s)	1960
BaeGI	GKGCMC	2 cut(s)	1145, 1598
BalI	TGGCCA	1 cut(s)	323
BanI	GGYRCC	3 cut(s)	649, 1595, 1669
BanII	GRGCYC	1 cut(s)	23
BarI	GAAGNNNNNNTAC	2 cut(s)	504, 536
BbsI	GAAGAC	2 cut(s)	1041, 1617
Bbv12I	GWGCWC	2 cut(s)	23, 297
BccI	CCATC	6 cut(s)	241, 308, 899, 1000, 1319, 1782
BceAI	ACGGC	1 cut(s)	1569
BciT130I	CCWGG	2 cut(s)	951, 1600
BclI	TGATCA	2 cut(s)	328, 1909
BcoDI	GTCTC	6 cut(s)	237, 739, 873, 1370, 1447, 1644
BfaI	CTAG	3 cut(s)	537, 825, 1244
BfoI	RGCGCY	2 cut(s)	543, 653
BisI	GCNGC	1 cut(s)	30
BlsI	GCNGC	1 cut(s)	31
Bme1390I	CCNGG	2 cut(s)	951, 1600
Bme18I	GGWCC	1 cut(s)	1960
BmgT120I	GGNCC	1 cut(s)	1960
BmiI	GGNNCC	3 cut(s)	651, 1597, 1671
BmrFI	CCNGG	2 cut(s)	951, 1600
BmsI	GCATC	5 cut(s)	724, 1000, 1089, 1319, 1648
BmtI	GCTAGC	1 cut(s)	540
BpiI	GAAGAC	2 cut(s)	1041, 1617
BpmI	CTGGAG	1 cut(s)	963
Bpu14I	TTCGAA	1 cut(s)	1116
BpuEI	CTTGAG	1 cut(s)	1202
BsaBI	GATNNNNATC	1 cut(s)	1014
BsaHI	GRCGYC	1 cut(s)	650
BsaJI	CCNNGG	2 cut(s)	438, 950
BsaWI	WCCGGW	2 cut(s)	121, 155
BsaXI	ACNNNNNCTCC	2 cut(s)	1377, 1407
Bsc4I	CCNNNNNNNGG	4 cut(s)	444, 558, 875, 1152
Bse1I	ACTGG	4 cut(s)	783, 1360, 1480, 1993
Bse3DI	GCAATG	1 cut(s)	1887
Bse8I	GATNNNNATC	1 cut(s)	1014
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	2 cut(s)	951, 1600
BseDI	CCNNGG	2 cut(s)	438, 950
BseJI	GATNNNNATC	1 cut(s)	1014
BseLI	CCNNNNNNNGG	4 cut(s)	444, 558, 875, 1152
BseMI	GCAATG	1 cut(s)	1887
BseMII	CTCAG	2 cut(s)	1413, 1727
BseNI	ACTGG	4 cut(s)	783, 1360, 1480, 1993
BseRI	GAGGAG	7 cut(s)	187, 356, 402, 966, 974, 1662, 1873
BseSI	GKGCMC	2 cut(s)	1145, 1598
BshFI	GGCC	2 cut(s)	323, 1680
BshNI	GGYRCC	3 cut(s)	649, 1595, 1669
BsiHKAI	GWGCWC	2 cut(s)	23, 297
BsiSI	CCGG	2 cut(s)	122, 156
BslFI	GGGAC	1 cut(s)	1464
BslI	CCNNNNNNNGG	4 cut(s)	444, 558, 875, 1152
BsmAI	GTCTC	6 cut(s)	237, 739, 873, 1370, 1447, 1644
BsmBI	CGTCTC	2 cut(s)	739, 873
BsmFI	GGGAC	1 cut(s)	1464
BsmI	GAATGC	1 cut(s)	1102
BsnI	GGCC	2 cut(s)	323, 1680
Bsp119I	TTCGAA	1 cut(s)	1116
Bsp1286I	GDGCHC	4 cut(s)	23, 297, 1145, 1598
Bsp13I	TCCGGA	1 cut(s)	155
Bsp143I	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
BspACI	CCGC	2 cut(s)	29, 1039
BspANI	GGCC	2 cut(s)	323, 1680
BspCNI	CTCAG	2 cut(s)	1414, 1726
BspEI	TCCGGA	1 cut(s)	155
BspHI	TCATGA	2 cut(s)	187, 367
BspLI	GGNNCC	3 cut(s)	651, 1597, 1671
BspOI	GCTAGC	1 cut(s)	540
BspPI	GGATC	5 cut(s)	220, 662, 1137, 1169, 1221
BspQI	GCTCTTC	1 cut(s)	11
BspT104I	TTCGAA	1 cut(s)	1116
BspT107I	GGYRCC	3 cut(s)	649, 1595, 1669
BsrDI	GCAATG	1 cut(s)	1887
BsrI	ACTGG	4 cut(s)	783, 1360, 1480, 1993
BssECI	CCNNGG	2 cut(s)	438, 950
BssMI	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
BssNI	GRCGYC	1 cut(s)	650
BssT1I	CCWWGG	1 cut(s)	438
Bst2UI	CCWGG	2 cut(s)	951, 1600
Bst4CI	ACNGT	6 cut(s)	659, 723, 1270, 1333, 1621, 1666
Bst6I	CTCTTC	4 cut(s)	11, 258, 779, 996
BstACI	GRCGYC	1 cut(s)	650
BstBI	TTCGAA	1 cut(s)	1116
BstC8I	GCNNGC	4 cut(s)	538, 1674, 1678, 1755
BstDEI	CTNAG	4 cut(s)	220, 1044, 1422, 1713
BstH2I	RGCGCY	2 cut(s)	543, 653
BstHHI	GCGC	4 cut(s)	542, 564, 652, 679
BstKTI	GATC	8 cut(s)	215, 331, 478, 670, 1132, 1177, 1216, 1912
BstMAI	GTCTC	6 cut(s)	237, 739, 873, 1370, 1447, 1644
BstMBI	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
BstMWI	GCNNNNNNNGC	1 cut(s)	946
BstNI	CCWGG	2 cut(s)	951, 1600
BstSCI	CCNGG	2 cut(s)	949, 1598
BstSLI	GKGCMC	2 cut(s)	1145, 1598
BstV2I	GAAGAC	2 cut(s)	1041, 1617
BstX2I	RGATCY	3 cut(s)	212, 1129, 1213
BstYI	RGATCY	3 cut(s)	212, 1129, 1213
BsuRI	GGCC	2 cut(s)	323, 1680
BtsI	GCAGTG	1 cut(s)	1307
BtsIMutI	CAGTG	4 cut(s)	719, 1307, 1473, 1635
Cac8I	GCNNGC	4 cut(s)	538, 1674, 1678, 1755
CciI	TCATGA	2 cut(s)	187, 367
CfoI	GCGC	4 cut(s)	542, 564, 652, 679
Cfr13I	GGNCC	1 cut(s)	1960
CsiI	ACCWGGT	1 cut(s)	1598
Csp6I	GTAC	2 cut(s)	1460, 1496
CviAII	CATG	5 cut(s)	188, 332, 368, 1589, 1913
CviQI	GTAC	2 cut(s)	1460, 1496
DdeI	CTNAG	4 cut(s)	220, 1044, 1422, 1713
DinI	GGCGCC	1 cut(s)	651
DpnI	GATC	8 cut(s)	214, 330, 477, 669, 1131, 1176, 1215, 1911
DpnII	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
DraI	TTTAAA	1 cut(s)	697
DraIII	CACNNNGTG	1 cut(s)	659
EaeI	YGGCCR	1 cut(s)	321
Eam1104I	CTCTTC	4 cut(s)	11, 258, 779, 996
EarI	CTCTTC	4 cut(s)	11, 258, 779, 996
Ecl136II	GAGCTC	1 cut(s)	21
Eco130I	CCWWGG	1 cut(s)	438
Eco24I	GRGCYC	1 cut(s)	23
Eco47I	GGWCC	1 cut(s)	1960
Eco47III	AGCGCT	1 cut(s)	541
Eco53kI	GAGCTC	1 cut(s)	21
Eco57I	CTGAAG	7 cut(s)	117, 183, 300, 417, 1208, 1611, 1749
EcoICRI	GAGCTC	1 cut(s)	21
EcoRI	GAATTC	1 cut(s)	983
EcoRII	CCWGG	2 cut(s)	949, 1598
EcoT14I	CCWWGG	1 cut(s)	438
EcoT22I	ATGCAT	1 cut(s)	1704
EcoT38I	GRGCYC	1 cut(s)	23
EgeI	GGCGCC	1 cut(s)	651
EheI	GGCGCC	1 cut(s)	651
ErhI	CCWWGG	1 cut(s)	438
Esp3I	CGTCTC	2 cut(s)	739, 873
FaeI	CATG	5 cut(s)	191, 335, 371, 1592, 1916
FalI	AAGNNNNNCTT	6 cut(s)	1097, 1129, 1446, 1478, 1503, 1535
FaqI	GGGAC	1 cut(s)	1464
FatI	CATG	5 cut(s)	187, 331, 367, 1588, 1912
FauNDI	CATATG	3 cut(s)	673, 1090, 1399
FbaI	TGATCA	2 cut(s)	328, 1909
FblI	GTMKAC	2 cut(s)	132, 1721
Fnu4HI	GCNGC	1 cut(s)	30
FriOI	GRGCYC	1 cut(s)	23
Fsp4HI	GCNGC	1 cut(s)	30
FspBI	CTAG	3 cut(s)	537, 825, 1244
GlaI	GCGC	4 cut(s)	541, 563, 651, 678
GluI	GCNGC	1 cut(s)	30
GsuI	CTGGAG	1 cut(s)	963
HaeII	RGCGCY	2 cut(s)	543, 653
HaeIII	GGCC	2 cut(s)	323, 1680
HapII	CCGG	2 cut(s)	122, 156
HhaI	GCGC	4 cut(s)	542, 564, 652, 679
Hin1I	GRCGYC	1 cut(s)	650
Hin1II	CATG	5 cut(s)	191, 335, 371, 1592, 1916
Hin6I	GCGC	4 cut(s)	540, 562, 650, 677
HinP1I	GCGC	4 cut(s)	540, 562, 650, 677
HindIII	AAGCTT	1 cut(s)	1856
HpaII	CCGG	2 cut(s)	122, 156
HphI	GGTGA	7 cut(s)	19, 52, 218, 671, 793, 1846, 1919
Hpy166II	GTNNAC	5 cut(s)	133, 148, 776, 1065, 1722
Hpy8I	GTNNAC	5 cut(s)	133, 148, 776, 1065, 1722
HpyCH4III	ACNGT	6 cut(s)	659, 723, 1270, 1333, 1621, 1666
HpyCH4IV	ACGT	1 cut(s)	1162
HpyCH4V	TGCA	5 cut(s)	1088, 1310, 1702, 1757, 1892
HpyF10VI	GCNNNNNNNGC	1 cut(s)	946
HpyF3I	CTNAG	4 cut(s)	220, 1044, 1422, 1713
HpySE526I	ACGT	1 cut(s)	1162
Hsp92I	GRCGYC	1 cut(s)	650
Hsp92II	CATG	5 cut(s)	191, 335, 371, 1592, 1916
HspAI	GCGC	4 cut(s)	540, 562, 650, 677
KasI	GGCGCC	1 cut(s)	649
Kpn2I	TCCGGA	1 cut(s)	155
Ksp22I	TGATCA	2 cut(s)	328, 1909
Kzo9I	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
LguI	GCTCTTC	1 cut(s)	11
LmnI	GCTCC	5 cut(s)	158, 415, 736, 1790, 1926
LweI	GCATC	5 cut(s)	724, 1000, 1089, 1319, 1648
MabI	ACCWGGT	1 cut(s)	1598
MaeI	CTAG	3 cut(s)	537, 825, 1244
MaeII	ACGT	1 cut(s)	1162
MaeIII	GTNAC	6 cut(s)	601, 659, 1256, 1270, 1393, 1759
MalI	GATC	8 cut(s)	214, 330, 477, 669, 1131, 1176, 1215, 1911
MboI	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
MfeI	CAATTG	1 cut(s)	423
MflI	RGATCY	3 cut(s)	212, 1129, 1213
MhlI	GDGCHC	4 cut(s)	23, 297, 1145, 1598
MlsI	TGGCCA	1 cut(s)	323
MluNI	TGGCCA	1 cut(s)	323
Mly113I	GGCGCC	1 cut(s)	650
MlyI	GAGTC	7 cut(s)	29, 128, 184, 245, 701, 878, 971
Mox20I	TGGCCA	1 cut(s)	323
Mph1103I	ATGCAT	1 cut(s)	1704
MroI	TCCGGA	1 cut(s)	155
MscI	TGGCCA	1 cut(s)	323
MseI	TTAA	9 cut(s)	495, 696, 815, 819, 1022, 1104, 1604, 1614, 1740
MslI	CAYNNNNRTG	1 cut(s)	336
Msp20I	TGGCCA	1 cut(s)	323
MspI	CCGG	2 cut(s)	122, 156
MspR9I	CCNGG	2 cut(s)	951, 1600
MunI	CAATTG	1 cut(s)	423
Mva1269I	GAATGC	1 cut(s)	1102
MvaI	CCWGG	2 cut(s)	951, 1600
MwoI	GCNNNNNNNGC	1 cut(s)	946
NarI	GGCGCC	1 cut(s)	650
NdeI	CATATG	3 cut(s)	673, 1090, 1399
NdeII	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
NheI	GCTAGC	1 cut(s)	536
NlaIII	CATG	5 cut(s)	191, 335, 371, 1592, 1916
NlaIV	GGNNCC	3 cut(s)	651, 1597, 1671
NmuCI	GTSAC	4 cut(s)	601, 659, 1256, 1393
NsiI	ATGCAT	1 cut(s)	1704
NspV	TTCGAA	1 cut(s)	1116
PacI	TTAATTAA	1 cut(s)	819
PagI	TCATGA	2 cut(s)	187, 367
PciSI	GCTCTTC	1 cut(s)	11
PcsI	WCGNNNNNNNCGW	1 cut(s)	1159
PctI	GAATGC	1 cut(s)	1102
PfeI	GAWTC	9 cut(s)	91, 140, 152, 389, 548, 750, 1181, 1411, 1573
PflFI	GACNNNGTC	1 cut(s)	1621
PflMI	CCANNNNNTGG	1 cut(s)	558
PkrI	GCNGC	1 cut(s)	31
PleI	GAGTC	7 cut(s)	29, 128, 183, 245, 701, 878, 970
PluTI	GGCGCC	1 cut(s)	653
PpsI	GAGTC	7 cut(s)	29, 128, 183, 245, 701, 878, 970
Psp124BI	GAGCTC	1 cut(s)	23
Psp6I	CCWGG	2 cut(s)	949, 1598
PspGI	CCWGG	2 cut(s)	949, 1598
PspN4I	GGNNCC	3 cut(s)	651, 1597, 1671
PspPI	GGNCC	1 cut(s)	1960
PsuI	RGATCY	3 cut(s)	212, 1129, 1213
PsyI	GACNNNGTC	1 cut(s)	1621
RsaI	GTAC	2 cut(s)	1461, 1497
RsaNI	GTAC	2 cut(s)	1460, 1496
RseI	CAYNNNNRTG	1 cut(s)	336
SacI	GAGCTC	1 cut(s)	23
SapI	GCTCTTC	1 cut(s)	11
SaqAI	TTAA	9 cut(s)	495, 696, 815, 819, 1022, 1104, 1604, 1614, 1740
SatI	GCNGC	1 cut(s)	30
Sau3AI	GATC	8 cut(s)	212, 328, 475, 667, 1129, 1174, 1213, 1909
Sau96I	GGNCC	1 cut(s)	1960
SchI	GAGTC	7 cut(s)	29, 128, 184, 245, 701, 878, 971
ScrFI	CCNGG	2 cut(s)	951, 1600
SduI	GDGCHC	4 cut(s)	23, 297, 1145, 1598
SexAI	ACCWGGT	1 cut(s)	1598
SfaNI	GCATC	5 cut(s)	724, 1000, 1089, 1319, 1648
SfoI	GGCGCC	1 cut(s)	651
SfuI	TTCGAA	1 cut(s)	1116
SinI	GGWCC	1 cut(s)	1960
SmiMI	CAYNNNNRTG	1 cut(s)	336
SmlI	CTYRAG	1 cut(s)	1217
SmoI	CTYRAG	1 cut(s)	1217
SsiI	CCGC	2 cut(s)	29, 1039
SspDI	GGCGCC	1 cut(s)	649
SspI	AATATT	1 cut(s)	1171
SspMI	CTAG	3 cut(s)	537, 825, 1244
SstI	GAGCTC	1 cut(s)	23
StyD4I	CCNGG	2 cut(s)	949, 1598
StyI	CCWWGG	1 cut(s)	438
TaaI	ACNGT	6 cut(s)	659, 723, 1270, 1333, 1621, 1666
TaiI	ACGT	1 cut(s)	1165
TaqI	TCGA	2 cut(s)	415, 1116
TatI	WGTACW	2 cut(s)	1459, 1495
TauI	GCSGC	1 cut(s)	32
TfiI	GAWTC	9 cut(s)	91, 140, 152, 389, 548, 750, 1181, 1411, 1573
Tru1I	TTAA	9 cut(s)	495, 696, 815, 819, 1022, 1104, 1604, 1614, 1740
Tru9I	TTAA	9 cut(s)	495, 696, 815, 819, 1022, 1104, 1604, 1614, 1740
TscAI	CASTG	4 cut(s)	726, 1307, 1480, 1635
TseFI	GTSAC	4 cut(s)	601, 659, 1256, 1393
Tsp45I	GTSAC	4 cut(s)	601, 659, 1256, 1393
TspGWI	ACGGA	2 cut(s)	911, 1420
TspRI	CASTG	4 cut(s)	726, 1307, 1480, 1635
Tth111I	GACNNNGTC	1 cut(s)	1621
Van91I	CCANNNNNTGG	1 cut(s)	558
VpaK11BI	GGWCC	1 cut(s)	1960
XapI	RAATTY	4 cut(s)	566, 983, 1338, 1940
XbaI	TCTAGA	1 cut(s)	1243
XmiI	GTMKAC	2 cut(s)	132, 1721
XspI	CTAG	3 cut(s)	537, 825, 1244
Zsp2I	ATGCAT	1 cut(s)	1704

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.