Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0002914

Physical Location & Seq

Genomic Coordinates Reverse (-)

5981861 .. 5985867

Gene Structure 4007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g05570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGTCTGATGGTGAAGGATCCGATTCGGAGGATTATCCGTCAATCAGAGACTCGGACTCAGAAGGCGAAGAGGATGATTTCTCCTCTGGAGATGAGGATGTTATCAACACAAGTGACAATGATGGTGTGGCCGGTGATTATGGTAACAGTGATGATGAAAGTGATAGTTCTGAACTTCATGAGGGAGTTGAGGAGAGTGATTCATCTGAGGATGAGGTGGTTCCTCGAAACACAGTCGGTGATGTTCCCTTGGAGTGGTATCGGGATGAGAAACATATTGGTTATGACATTAAAGGGAAGAAGATAAAGAAGAAGGAAAAAGCAGATAAATTGCAATCCTTTCTTGCTAGTGCTGACGATTCAAAGAACTGGCGCAAAATTTATGATGAATATAATGATGAGGAAGTTGAACTGACAAAAGAAGATATCAAATCAATTAGTAGACTGCTCGAAGGAAAGGCTCCACATGGTGACTTTGATCCATATGCGCCATATGTTGATTGGTTTAAATGGGATGACTCCAACCATCCACTGTCAAATGCACCTGAACCAAAGAGACGATTCATTCCTTCAAAATCTGAAAGTAAACTGGTTAATAAGATTAAGTTGGCCATTCGTAAGGGTTTAATTAAGCCTAAAAAGTCCAAAGAGGAAGAAGAAGAAGAAGAAATTTATCCACTGTGGGAAGACGACTCTAATACAACGGAAAAGGATAACCATCTATCTTACATTCCTGCACCAAAGCCCAAATTGCCTGGCCATGAGGAGTCCTTCAATCCATCTTTAGAATACATCCCAACACAAGAAGAGATCAACTCTTACCAGTTAATGTATGAAGAAGACCGTCCTAAATTTATACCTAAAAGATTTGCAAATATGAGAAGCATCCCTGCATATGAGAATGCTGTGAAGGAATGCTTTGAGAGATGTTTGGATCTATATTTGTGCCCCAGAGTTCGAAAAAAACGTCTTAATATCGATCCTGAATCTCTGAAGCCCAAGCTACCAAGCCGAACTGAACTTAAGCCTTACCCTGTAGCATGTTATCTTGAGTATAGAGGTCACAACGATGCAGTTACATCTGTTTCTGTAGAAGCCTCAGGGCAGTGGATTGCATCAGGTTCTTTGGATGGAACTATGCGTATTTGGGAGGTCGAAACTGGTAGATGTGTCAAGATCTGCGAGATTGGTGAAGCCGTCAAATATGTATCTTGGAATCCTAATCCTGAGCATTCTATAGTGGCTGTCTCGGCGGGACAAGATGTACTTCTTTTGAACACTGGTTGTGGGGATGAAGAAGTACAGAAAAGAACCAAAGAACTTCTCTCTGTTGAGATGCCTATGCCCCATGACTCTGGCAAAACAGCATCTAGTGTAAGCTGGCTTCATGATGATAAACTTGAGGGGGTCAGATTAAGGCATTCCAAGACCGTGGCTTCGGTAGAATGGCATCGTAAAGGAGACTACCTTTCGACAGTAATGCCACAAGAGCCTTTTTTATACACAAGCTCTCTAAGAAGTTTACTCAGACACTTTCATTCAAGTTGCATGGGATTGCAGTTACTTCAGTTTTCCATCCTACACGGTCTTTCTTCTTCATTTCAACAAAGAAGACCGTTCGTGTTTATGATCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030684 GO:0030686 GO:0030687 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044877 GO:0046483 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BOP1NT WD40

Protein Analysis

546

Amino Acids

62.28

Weight (kDa)

4.95

Isoelectric Point (pI)

54.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BOP1NT	PF08145	87 - 345	4.9e-100	BOP1NT (NUC169) domain
Beta-prop_Aladin	PF25460	347 - 415	6.5e-06	Aladin seven-bladed propeller
WD40	PF00400	348 - 383	3.6e-09	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	348 - 419	7.1e-06	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	350 - 428	8.5e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	351 - 407	9.5e-08	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	351 - 415	2.9e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	351 - 410	7.2e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	352 - 426	8.5e-12	WDR5 beta-propeller domain
EIF3I	PF24805	352 - 417	9.2e-10	EIF3I
Beta-prop_CAF1B_HIR1	PF24105	352 - 470	3.3e-08	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	355 - 430	1.9e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40360 AT2G40360
fragaria_vesca	FvH4_5g21650 FvH4_5g21650 FvH4_5g21650
malus_domestica	MD05G1062400.v1.1 MD10G1070100.v1.1
prunus_persica	Prupe.6G114000_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1
pyrus_communis	pycom05g05370 pycom05g05390 pycom10g05570
rosa_chinensis	RchiOBHm_Chr7g0209131 RchiOBHm_Chr7g0241521 RchiOBHm_Chr7g0241531 RchiOBHm_Chr7g0241541
rosa_laevigata	RLG00000000568
rosa_multiflora	Rmu_sc0000821.1_g000025 Rmu_sc0002268.1_g000023
rosa_roxburghii	Rroxscaffold_3G00218950 Rroxscaffold_3G00249700
rosa_rugosa	Rorug07G0109700 Rorug07G0109800 Rorug07G0345000
rosa_samantha	Rh7AG243600 Rh7AG499500 Rh7AG499600 Rh7BG238700 Rh7BG470600 Rh7BG470700 Rh7CG259100 Rh7DG250600 Rh7DG484000 Rh7DG484200
rosa_wichuraiana	Rw7G020660 Rw7G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	442
AciI	CCGC	1 cut(s)	1253
AclWI	GGATC	5 cut(s)	12, 25, 473, 942, 974
AcoI	YGGCCR	3 cut(s)	129, 609, 757
AcsI	RAATTY	3 cut(s)	378, 669, 851
AcuI	CTGAAG	2 cut(s)	1013, 1550
AdeI	CACNNNGTG	1 cut(s)	470
AfaI	GTAC	2 cut(s)	1266, 1302
AfiI	CCNNNNNNNGG	1 cut(s)	255
AflII	CTTAAG	1 cut(s)	1022
AgsI	TTSAA	7 cut(s)	363, 410, 573, 775, 1276, 1542, 1604
AjnI	CCWGG	1 cut(s)	754
AluBI	AGCT	3 cut(s)	1003, 1380, 1509
AluI	AGCT	3 cut(s)	1003, 1380, 1509
Alw26I	GTCTC	4 cut(s)	42, 550, 1252, 1455
AlwI	GGATC	5 cut(s)	12, 25, 473, 942, 974
AoxI	GGCC	3 cut(s)	129, 609, 757
ApoI	RAATTY	3 cut(s)	378, 669, 851
ArsI	GACNNNNNNTTYG	4 cut(s)	952, 984, 1420, 1452
AspLEI	GCGC	2 cut(s)	375, 490
AsuHPI	GGTGA	5 cut(s)	23, 146, 251, 482, 1202
AsuII	TTCGAA	1 cut(s)	958
AxyI	CCTNAGG	1 cut(s)	1099
BaeGI	GKGCMC	1 cut(s)	950
BalI	TGGCCA	2 cut(s)	611, 759
BamHI	GGATCC	1 cut(s)	17
BarI	GAAGNNNNNNTAC	2 cut(s)	1368, 1400
BbsI	GAAGAC	3 cut(s)	693, 846, 1618
BccI	CCATC	7 cut(s)	2, 116, 534, 726, 787, 1124, 1583
BceAI	ACGGC	1 cut(s)	1181
BcgI	CGANNNNNNTGC	2 cut(s)	1462, 1496
BciT130I	CCWGG	1 cut(s)	756
BcoDI	GTCTC	4 cut(s)	42, 550, 1252, 1455
BfaI	CTAG	2 cut(s)	348, 1371
BfmI	CTRYAG	3 cut(s)	1035, 1089, 1236
BfrI	CTTAAG	1 cut(s)	1022
BglII	AGATCT	1 cut(s)	1176
Bme1390I	CCNGG	1 cut(s)	756
BmiI	GGNNCC	3 cut(s)	19, 222, 462
BmrFI	CCNGG	1 cut(s)	756
BmsI	GCATC	6 cut(s)	894, 1060, 1124, 1326, 1376, 1459
BpiI	GAAGAC	3 cut(s)	693, 846, 1618
BplI	GAGNNNNNCTC	2 cut(s)	800, 832
BpmI	CTGGAG	1 cut(s)	108
Bpu10I	CCTNAGC	1 cut(s)	1227
Bpu14I	TTCGAA	1 cut(s)	958
BpuEI	CTTGAG	2 cut(s)	1070, 1421
Bsa29I	ATCGAT	1 cut(s)	978
BsaBI	GATNNNNATC	1 cut(s)	717
BsaJI	CCNNGG	2 cut(s)	249, 1431
Bsc4I	CCNNNNNNNGG	1 cut(s)	255
Bse118I	RCCGGY	1 cut(s)	131
Bse1I	ACTGG	5 cut(s)	374, 594, 823, 1165, 1285
Bse21I	CCTNAGG	1 cut(s)	1099
Bse8I	GATNNNNATC	1 cut(s)	717
BseBI	CCWGG	1 cut(s)	756
BseCI	ATCGAT	1 cut(s)	978
BseDI	CCNNGG	2 cut(s)	249, 1431
BseJI	GATNNNNATC	1 cut(s)	717
BseLI	CCNNNNNNNGG	1 cut(s)	255
BseMII	CTCAG	5 cut(s)	72, 198, 1113, 1218, 1540
BseNI	ACTGG	5 cut(s)	374, 594, 823, 1165, 1285
BseRI	GAGGAG	3 cut(s)	73, 206, 779
BseSI	GKGCMC	1 cut(s)	950
BsgI	GTGCAG	1 cut(s)	720
BshFI	GGCC	3 cut(s)	131, 611, 759
BshVI	ATCGAT	1 cut(s)	978
BsiSI	CCGG	1 cut(s)	132
BslFI	GGGAC	1 cut(s)	1269
BslI	CCNNNNNNNGG	1 cut(s)	255
BsmAI	GTCTC	4 cut(s)	42, 550, 1252, 1455
BsmBI	CGTCTC	1 cut(s)	550
BsmFI	GGGAC	1 cut(s)	1269
BsmI	GAATGC	4 cut(s)	907, 920, 1231, 1420
BsnI	GGCC	3 cut(s)	131, 611, 759
Bsp119I	TTCGAA	1 cut(s)	958
Bsp1286I	GDGCHC	1 cut(s)	950
Bsp143I	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
BspACI	CCGC	1 cut(s)	1253
BspANI	GGCC	3 cut(s)	131, 611, 759
BspCNI	CTCAG	5 cut(s)	71, 199, 1112, 1219, 1539
BspDI	ATCGAT	1 cut(s)	978
BspHI	TCATGA	2 cut(s)	178, 1387
BspLI	GGNNCC	3 cut(s)	19, 222, 462
BspPI	GGATC	5 cut(s)	12, 25, 473, 942, 974
BspT104I	TTCGAA	1 cut(s)	958
BspTI	CTTAAG	1 cut(s)	1022
BsrFI	RCCGGY	1 cut(s)	131
BsrI	ACTGG	5 cut(s)	374, 594, 823, 1165, 1285
BssAI	RCCGGY	1 cut(s)	131
BssECI	CCNNGG	2 cut(s)	249, 1431
BssMI	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
BssT1I	CCWWGG	1 cut(s)	249
Bst2UI	CCWGG	1 cut(s)	756
Bst4CI	ACNGT	9 cut(s)	149, 235, 534, 681, 845, 1432, 1477, 1586, 1617
Bst6I	CTCTTC	2 cut(s)	63, 801
BstAFI	CTTAAG	1 cut(s)	1022
BstBI	TTCGAA	1 cut(s)	958
BstC8I	GCNNGC	1 cut(s)	1382
BstDEI	CTNAG	6 cut(s)	58, 207, 1099, 1227, 1514, 1526
BstDSI	CCRYGG	1 cut(s)	1431
BstHHI	GCGC	2 cut(s)	375, 490
BstKTI	GATC	7 cut(s)	20, 481, 813, 937, 982, 1179, 1632
BstMAI	GTCTC	4 cut(s)	42, 550, 1252, 1455
BstMBI	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
BstMWI	GCNNNNNNNGC	3 cut(s)	751, 1250, 1489
BstNI	CCWGG	1 cut(s)	756
BstNSI	RCATGY	1 cut(s)	1044
BstSCI	CCNGG	1 cut(s)	754
BstSFI	CTRYAG	3 cut(s)	1035, 1089, 1236
BstSLI	GKGCMC	1 cut(s)	950
BstV2I	GAAGAC	3 cut(s)	693, 846, 1618
BstX2I	RGATCY	3 cut(s)	17, 934, 1176
BstXI	CCANNNNNNTGG	2 cut(s)	1355, 1432
BstYI	RGATCY	3 cut(s)	17, 934, 1176
Bsu15I	ATCGAT	1 cut(s)	978
Bsu36I	CCTNAGG	1 cut(s)	1099
BsuRI	GGCC	3 cut(s)	131, 611, 759
BsuTUI	ATCGAT	1 cut(s)	978
BtgI	CCRYGG	1 cut(s)	1431
BtsI	GCAGTG	1 cut(s)	1112
BtsIMutI	CAGTG	5 cut(s)	154, 530, 677, 1112, 1278
Cac8I	GCNNGC	1 cut(s)	1382
CciI	TCATGA	2 cut(s)	178, 1387
CfoI	GCGC	2 cut(s)	375, 490
Cfr10I	RCCGGY	1 cut(s)	131
ClaI	ATCGAT	1 cut(s)	978
Csp6I	GTAC	2 cut(s)	1265, 1301
CviAII	CATG	7 cut(s)	179, 467, 761, 1041, 1349, 1388, 1549
CviQI	GTAC	2 cut(s)	1265, 1301
DdeI	CTNAG	6 cut(s)	58, 207, 1099, 1227, 1514, 1526
DpnI	GATC	7 cut(s)	19, 480, 812, 936, 981, 1178, 1631
DpnII	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
DraI	TTTAAA	1 cut(s)	508
DraIII	CACNNNGTG	1 cut(s)	470
EaeI	YGGCCR	3 cut(s)	129, 609, 757
Eam1104I	CTCTTC	2 cut(s)	63, 801
EarI	CTCTTC	2 cut(s)	63, 801
Eco130I	CCWWGG	1 cut(s)	249
Eco32I	GATATC	1 cut(s)	427
Eco57I	CTGAAG	2 cut(s)	1013, 1550
Eco81I	CCTNAGG	1 cut(s)	1099
EcoRII	CCWGG	1 cut(s)	754
EcoRV	GATATC	1 cut(s)	427
EcoT14I	CCWWGG	1 cut(s)	249
ErhI	CCWWGG	1 cut(s)	249
Esp3I	CGTCTC	1 cut(s)	550
FaeI	CATG	7 cut(s)	182, 470, 764, 1044, 1352, 1391, 1552
FalI	AAGNNNNNCTT	4 cut(s)	902, 934, 1251, 1283
FaqI	GGGAC	1 cut(s)	1269
FatI	CATG	7 cut(s)	178, 466, 760, 1040, 1348, 1387, 1548
FauI	CCCGC	1 cut(s)	1246
FauNDI	CATATG	3 cut(s)	484, 493, 895
FblI	GTMKAC	1 cut(s)	442
FspBI	CTAG	2 cut(s)	348, 1371
GlaI	GCGC	2 cut(s)	374, 489
GsuI	CTGGAG	1 cut(s)	108
HaeIII	GGCC	3 cut(s)	131, 611, 759
HapII	CCGG	1 cut(s)	132
HhaI	GCGC	2 cut(s)	375, 490
Hin1II	CATG	7 cut(s)	182, 470, 764, 1044, 1352, 1391, 1552
Hin6I	GCGC	2 cut(s)	373, 488
HinP1I	GCGC	2 cut(s)	373, 488
HpaII	CCGG	1 cut(s)	132
HphI	GGTGA	5 cut(s)	23, 146, 251, 482, 1202
Hpy166II	GTNNAC	3 cut(s)	443, 587, 1523
Hpy188III	TCNNGA	8 cut(s)	87, 179, 263, 983, 1049, 1174, 1226, 1388
Hpy8I	GTNNAC	3 cut(s)	443, 587, 1523
HpyAV	CCTTC	7 cut(s)	8, 56, 307, 446, 579, 781, 904
HpyCH4III	ACNGT	9 cut(s)	149, 235, 534, 681, 845, 1432, 1477, 1586, 1617
HpyCH4IV	ACGT	1 cut(s)	967
HpyCH4V	TGCA	9 cut(s)	334, 542, 737, 872, 893, 1073, 1115, 1548, 1558
HpyF10VI	GCNNNNNNNGC	3 cut(s)	751, 1250, 1489
HpyF3I	CTNAG	6 cut(s)	58, 207, 1099, 1227, 1514, 1526
HpySE526I	ACGT	1 cut(s)	967
Hsp92II	CATG	7 cut(s)	182, 470, 764, 1044, 1352, 1391, 1552
HspAI	GCGC	2 cut(s)	373, 488
Kzo9I	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
LmnI	GCTCC	1 cut(s)	466
LweI	GCATC	6 cut(s)	894, 1060, 1124, 1326, 1376, 1459
MaeI	CTAG	2 cut(s)	348, 1371
MaeII	ACGT	1 cut(s)	967
MaeIII	GTNAC	6 cut(s)	113, 143, 470, 1061, 1075, 1560
MalI	GATC	7 cut(s)	19, 480, 812, 936, 981, 1178, 1631
MboI	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
MflI	RGATCY	3 cut(s)	17, 934, 1176
MhlI	GDGCHC	1 cut(s)	950
MlsI	TGGCCA	2 cut(s)	611, 759
MluCI	AATT	7 cut(s)	329, 378, 435, 627, 669, 749, 851
MluNI	TGGCCA	2 cut(s)	611, 759
MlyI	GAGTC	6 cut(s)	44, 50, 512, 686, 776, 1346
MmeI	TCCRAC	1 cut(s)	546
Mox20I	TGGCCA	2 cut(s)	611, 759
MscI	TGGCCA	2 cut(s)	611, 759
MslI	CAYNNNNRTG	1 cut(s)	1068
Msp20I	TGGCCA	2 cut(s)	611, 759
MspCI	CTTAAG	1 cut(s)	1022
MspI	CCGG	1 cut(s)	132
MspR9I	CCNGG	1 cut(s)	756
Mva1269I	GAATGC	4 cut(s)	907, 920, 1231, 1420
MvaI	CCWGG	1 cut(s)	756
MwoI	GCNNNNNNNGC	3 cut(s)	751, 1250, 1489
NdeI	CATATG	3 cut(s)	484, 493, 895
NdeII	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
NlaIII	CATG	7 cut(s)	182, 470, 764, 1044, 1352, 1391, 1552
NlaIV	GGNNCC	3 cut(s)	19, 222, 462
NmeAIII	GCCGAG	1 cut(s)	1229
NmuCI	GTSAC	3 cut(s)	113, 470, 1061
NspI	RCATGY	1 cut(s)	1044
NspV	TTCGAA	1 cut(s)	958
PacI	TTAATTAA	1 cut(s)	630
PagI	TCATGA	2 cut(s)	178, 1387
PcsI	WCGNNNNNNNCGW	1 cut(s)	964
PctI	GAATGC	4 cut(s)	907, 920, 1231, 1420
PfeI	GAWTC	6 cut(s)	23, 200, 359, 561, 986, 1216
PleI	GAGTC	6 cut(s)	44, 50, 512, 686, 775, 1346
PpsI	GAGTC	6 cut(s)	44, 50, 512, 686, 775, 1346
Psp6I	CCWGG	1 cut(s)	754
PspGI	CCWGG	1 cut(s)	754
PspN4I	GGNNCC	3 cut(s)	19, 222, 462
PsuI	RGATCY	3 cut(s)	17, 934, 1176
RsaI	GTAC	2 cut(s)	1266, 1302
RsaNI	GTAC	2 cut(s)	1265, 1301
RseI	CAYNNNNRTG	1 cut(s)	1068
Sau3AI	GATC	7 cut(s)	17, 478, 810, 934, 979, 1176, 1629
SchI	GAGTC	6 cut(s)	44, 50, 512, 686, 776, 1346
ScrFI	CCNGG	1 cut(s)	756
SduI	GDGCHC	1 cut(s)	950
SfaNI	GCATC	6 cut(s)	894, 1060, 1124, 1326, 1376, 1459
SfcI	CTRYAG	3 cut(s)	1035, 1089, 1236
SfuI	TTCGAA	1 cut(s)	958
SmiMI	CAYNNNNRTG	1 cut(s)	1068
SmlI	CTYRAG	3 cut(s)	1022, 1049, 1400
SmoI	CTYRAG	3 cut(s)	1022, 1049, 1400
Sse9I	AATT	7 cut(s)	329, 378, 435, 627, 669, 749, 851
SsiI	CCGC	1 cut(s)	1253
SspMI	CTAG	2 cut(s)	348, 1371
StyD4I	CCNGG	1 cut(s)	754
StyI	CCWWGG	1 cut(s)	249
TaaI	ACNGT	9 cut(s)	149, 235, 534, 681, 845, 1432, 1477, 1586, 1617
TaiI	ACGT	1 cut(s)	970
TaqI	TCGA	6 cut(s)	226, 450, 958, 978, 1155, 1472
TasI	AATT	7 cut(s)	329, 378, 435, 627, 669, 749, 851
TatI	WGTACW	2 cut(s)	1264, 1300
TfiI	GAWTC	6 cut(s)	23, 200, 359, 561, 986, 1216
TscAI	CASTG	5 cut(s)	154, 537, 684, 1112, 1285
TseFI	GTSAC	3 cut(s)	113, 470, 1061
Tsp45I	GTSAC	3 cut(s)	113, 470, 1061
TspGWI	ACGGA	2 cut(s)	27, 719
TspRI	CASTG	5 cut(s)	154, 537, 684, 1112, 1285
Vha464I	CTTAAG	1 cut(s)	1022
XapI	RAATTY	3 cut(s)	378, 669, 851
XceI	RCATGY	1 cut(s)	1044
XcmI	CCANNNNNNNNNTGG	1 cut(s)	498
XmiI	GTMKAC	1 cut(s)	442
XspI	CTAG	2 cut(s)	348, 1371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.