Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002713

Physical Location & Seq

Genomic Coordinates Reverse (-)

35619443 .. 35623726

Gene Structure 4284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1261900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGTCCGACGAGCCTGCTCCCAGTCGCTGGTCCTTTCAGTTTGGTAGAAAAAAAGCACCGGAGCCGCAGCAAAACGGGCAGTCCACTAATGGAGATGTGAGTAATGGAAGTAGTTCCGGAGTGACCGCGAAGAGGACAGCTGACTTGGCCATCTACGAGCAATTTCAAAACCAGGGTAGGGGCTCCGTGCCTCACTCAAATGGAGTCTTAACTGATCAGCATGATGAGATACCGCAGAAGTCCCTTTTACCTGCTTTTGAATCAGCAGAAATGCGTTCTTTAGGAGAGAGTTTATGCAGGGATATAATTCGGGGTAGTCCAGATGTTAAGTGGGAAAGCATCAAGGGATTAGAGAATGCCAAGCGTTTGCTCAAAGAAGCAGTGGTCATGCCAATCAAATATCCCAAGTACTTTACTGGACTTTTAACACCGTGGAAAGGTATTCTCCTTTTTGGACCTCCAGGGACAGGAAAGACAATGCTTGCCAAGGCTGTTGCAACAGAGTGCAAGACCACATTTTTCAACATTTCAGCATCATCTGTTGTCAGTAAATGGCGCGGCGACTCAGAGAAGTTAGTGAAGGTGTTATTTGAGCTTGCTAGGCATCATGCGCCATCAACTATATTTATTGATGAAATTGACGCAATTATTAGTCAACGTGGTGAAGGACGAAGTGAGCATGAAGCAAGTAGGCGTCTGAAGACAGAGCTACTCGTACAGATGGATGGTTTGACGAAGACAAGTGAACTTGTTTTTGTTTTGGCTGCTACAAATCTTCCCTGGGAACTGGATGCAGCCATGCTCCGGCGTCTTGAGAAGCGAATTCTTGTGCCTCTGCCTGAACCAGAAGCAAGAAGAGCAATGTTCGAGGAGCTCCTGCCAGTACAGCCTGATGAGGAGAAGCTTCCTTATGATTTGTTGGTGGATAAAACTGAAGGATATTCAGGTTCAGATATTCGATTGGTGTGCAAGGAGGCTGCTATGCAGCCTCTGAGACGCATAATGACATTCCTTGAAGACAAAGAGGACACGGTTCCTGAGGATGAACTGCCCAAGGTTGGACCGATCAAACACGAAGATATTGAGACGGCTTTGAAGAACACAAGGCCGTCTGCTCATCTCCAAGCTCATCGTTATGAGAAGTTCAATGCTGATTATGGTAGTCAAATACTCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000922 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0015630 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA

Protein Analysis

393

Amino Acids

43.89

Weight (kDa)

6.0

Isoelectric Point (pI)

52.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	148 - 279	2e-41	ATPase family associated with various cellular activities (AAA)
RuvB_N	PF05496	148 - 216	4e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA_lid_3	PF17862	305 - 341	3.6e-12	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002713)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34560 AT2G34560
fragaria_vesca	FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g24600
malus_domestica	MD05G1283700.v1.1 MD10G1261900.v1.1 MD11G1161400.v1.1 MD17G1012100.v1.1
prunus_persica	Prupe.4G080800_v2.0.a1 Prupe.4G080800_v2.0.a1 Prupe.6G127100_v2.0.a1
pyrus_communis	pycom05g26280 pycom07g21360 pycom10g21820 pycom11g13380
rosa_chinensis	RchiOBHm_Chr5g0014221 RchiOBHm_Chr5g0044151
rosa_laevigata	RLG00000032091 RLG00000034247
rosa_multiflora	Rmu_sc0000939.1_g000032 Rmu_sc0014980.1_g000003 Rmu_ssc0000120.1_g000006
rosa_roxburghii	Rroxscaffold_1G00036900 Rroxscaffold_1G00062360
rosa_rugosa	Rorug05G0016300 Rorug05G0210900 Rorug05G0211000
rosa_samantha	Rh5AG295800 Rh5BG303600 Rh5CG119300 Rh5CG330600 Rh5DG312400
rosa_wichuraiana	Rw5G009640 Rw5G027350 Rw5G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	259
AccB7I	CCANNNNNTGG	1 cut(s)	27
AccII	CGCG	2 cut(s)	128, 558
AccIII	TCCGGA	1 cut(s)	116
AciI	CCGC	4 cut(s)	65, 126, 233, 558
AcoI	YGGCCR	1 cut(s)	147
AcsI	RAATTY	1 cut(s)	822
AcuI	CTGAAG	2 cut(s)	719, 954
AcyI	GRCGYC	2 cut(s)	694, 808
AfaI	GTAC	3 cut(s)	410, 717, 885
AfiI	CCNNNNNNNGG	7 cut(s)	27, 132, 178, 437, 467, 804, 1058
AgsI	TTSAA	6 cut(s)	167, 260, 523, 1016, 1096, 1147
AjnI	CCWGG	3 cut(s)	171, 460, 779
AluBI	AGCT	6 cut(s)	140, 595, 709, 874, 904, 1127
AluI	AGCT	6 cut(s)	140, 595, 709, 874, 904, 1127
Alw21I	GWGCWC	1 cut(s)	876
Alw26I	GTCTC	2 cut(s)	988, 1079
AlwNI	CAGNNNCTG	2 cut(s)	27, 991
Aor13HI	TCCGGA	1 cut(s)	116
AoxI	GGCC	2 cut(s)	147, 1106
ApeKI	GCWGC	5 cut(s)	67, 764, 794, 977, 985
ApoI	RAATTY	1 cut(s)	822
Asp700I	GAANNNNTTC	1 cut(s)	112
AspLEI	GCGC	2 cut(s)	558, 613
AspS9I	GGNCC	3 cut(s)	30, 455, 1061
AsuHPI	GGTGA	1 cut(s)	674
AvaII	GGWCC	3 cut(s)	30, 455, 1061
AxyI	CCTNAGG	1 cut(s)	1038
BalI	TGGCCA	1 cut(s)	149
BanII	GRGCYC	2 cut(s)	185, 876
BbsI	GAAGAC	3 cut(s)	707, 743, 1023
Bbv12I	GWGCWC	1 cut(s)	876
BbvI	GCAGC	5 cut(s)	79, 751, 806, 964, 997
BccI	CCATC	4 cut(s)	158, 622, 715, 719
BceAI	ACGGC	2 cut(s)	1093, 1104
BcgI	CGANNNNNNTGC	3 cut(s)	30, 948, 982
BciT130I	CCWGG	3 cut(s)	173, 462, 781
BclI	TGATCA	1 cut(s)	214
BcoDI	GTCTC	2 cut(s)	988, 1079
BfaI	CTAG	1 cut(s)	600
BfuAI	ACCTGC	1 cut(s)	259
BisI	GCNGC	7 cut(s)	65, 68, 559, 765, 795, 978, 986
BlsI	GCNGC	7 cut(s)	66, 69, 560, 766, 796, 979, 987
BmcAI	AGTACT	1 cut(s)	410
Bme1390I	CCNGG	3 cut(s)	173, 462, 781
Bme18I	GGWCC	3 cut(s)	30, 455, 1061
BmgT120I	GGNCC	3 cut(s)	30, 455, 1061
BmiI	GGNNCC	3 cut(s)	63, 184, 1035
BmrFI	CCNGG	3 cut(s)	173, 462, 781
BmrI	ACTGGG	1 cut(s)	15
BmsI	GCATC	4 cut(s)	348, 542, 613, 781
BmuI	ACTGGG	1 cut(s)	15
BpiI	GAAGAC	3 cut(s)	707, 743, 1023
BpmI	CTGGAG	1 cut(s)	444
BpuEI	CTTGAG	1 cut(s)	833
BsaHI	GRCGYC	2 cut(s)	694, 808
BsaJI	CCNNGG	7 cut(s)	172, 431, 461, 486, 779, 780, 1053
BsaWI	WCCGGW	2 cut(s)	58, 116
Bsc4I	CCNNNNNNNGG	7 cut(s)	27, 132, 178, 437, 467, 804, 1058
Bse1I	ACTGG	4 cut(s)	21, 421, 792, 881
Bse21I	CCTNAGG	1 cut(s)	1038
Bse3DI	GCAATG	1 cut(s)	867
BseAI	TCCGGA	1 cut(s)	116
BseBI	CCWGG	3 cut(s)	173, 462, 781
BseDI	CCNNGG	7 cut(s)	172, 431, 461, 486, 779, 780, 1053
BseGI	GGATG	3 cut(s)	730, 796, 1048
BseLI	CCNNNNNNNGG	7 cut(s)	27, 132, 178, 437, 467, 804, 1058
BseMI	GCAATG	1 cut(s)	867
BseMII	CTCAG	3 cut(s)	579, 983, 1029
BseNI	ACTGG	4 cut(s)	21, 421, 792, 881
BseRI	GAGGAG	2 cut(s)	884, 911
BseXI	GCAGC	5 cut(s)	79, 751, 806, 964, 997
Bsh1236I	CGCG	2 cut(s)	128, 558
BshFI	GGCC	2 cut(s)	149, 1108
BsiHKAI	GWGCWC	1 cut(s)	876
BsiSI	CCGG	3 cut(s)	59, 117, 805
BslFI	GGGAC	2 cut(s)	226, 478
BslI	CCNNNNNNNGG	7 cut(s)	27, 132, 178, 437, 467, 804, 1058
BsmAI	GTCTC	2 cut(s)	988, 1079
BsmBI	CGTCTC	2 cut(s)	988, 1079
BsmFI	GGGAC	2 cut(s)	226, 478
BsmI	GAATGC	1 cut(s)	361
BsnI	GGCC	2 cut(s)	149, 1108
Bsp1286I	GDGCHC	2 cut(s)	185, 876
Bsp13I	TCCGGA	1 cut(s)	116
Bsp143I	GATC	2 cut(s)	214, 1065
BspACI	CCGC	4 cut(s)	65, 126, 233, 558
BspANI	GGCC	2 cut(s)	149, 1108
BspCNI	CTCAG	3 cut(s)	578, 984, 1030
BspEI	TCCGGA	1 cut(s)	116
BspFNI	CGCG	2 cut(s)	128, 558
BspLI	GGNNCC	3 cut(s)	63, 184, 1035
BspMI	ACCTGC	1 cut(s)	259
BspQI	GCTCTTC	1 cut(s)	850
BsrDI	GCAATG	1 cut(s)	867
BsrI	ACTGG	4 cut(s)	21, 421, 792, 881
BssECI	CCNNGG	7 cut(s)	172, 431, 461, 486, 779, 780, 1053
BssMI	GATC	2 cut(s)	214, 1065
BssNI	GRCGYC	2 cut(s)	694, 808
BssT1I	CCWWGG	2 cut(s)	486, 1053
Bst2UI	CCWGG	3 cut(s)	173, 462, 781
Bst4CI	ACNGT	2 cut(s)	432, 1033
Bst6I	CTCTTC	2 cut(s)	125, 850
BstACI	GRCGYC	2 cut(s)	694, 808
BstC8I	GCNNGC	3 cut(s)	15, 483, 597
BstDEI	CTNAG	3 cut(s)	565, 992, 1038
BstDSI	CCRYGG	1 cut(s)	431
BstF5I	GGATG	3 cut(s)	730, 796, 1048
BstFNI	CGCG	2 cut(s)	128, 558
BstHHI	GCGC	2 cut(s)	558, 613
BstKTI	GATC	2 cut(s)	217, 1068
BstMAI	GTCTC	2 cut(s)	988, 1079
BstMBI	GATC	2 cut(s)	214, 1065
BstMWI	GCNNNNNNNGC	6 cut(s)	76, 146, 601, 610, 857, 886
BstNI	CCWGG	3 cut(s)	173, 462, 781
BstSCI	CCNGG	3 cut(s)	171, 460, 779
BstUI	CGCG	2 cut(s)	128, 558
BstV1I	GCAGC	5 cut(s)	79, 751, 806, 964, 997
BstV2I	GAAGAC	3 cut(s)	707, 743, 1023
Bsu36I	CCTNAGG	1 cut(s)	1038
BsuRI	GGCC	2 cut(s)	149, 1108
BtgI	CCRYGG	1 cut(s)	431
BtsCI	GGATG	3 cut(s)	730, 796, 1048
BtsI	GCAGTG	1 cut(s)	387
BtsIMutI	CAGTG	1 cut(s)	387
BveI	ACCTGC	1 cut(s)	259
Cac8I	GCNNGC	3 cut(s)	15, 483, 597
CaiI	CAGNNNCTG	2 cut(s)	27, 991
CfoI	GCGC	2 cut(s)	558, 613
Cfr13I	GGNCC	3 cut(s)	30, 455, 1061
CseI	GACGC	4 cut(s)	650, 683, 797, 1005
Csp6I	GTAC	3 cut(s)	409, 716, 884
CviAII	CATG	5 cut(s)	221, 388, 608, 680, 799
CviQI	GTAC	3 cut(s)	409, 716, 884
DdeI	CTNAG	3 cut(s)	565, 992, 1038
DpnI	GATC	2 cut(s)	216, 1067
DpnII	GATC	2 cut(s)	214, 1065
EaeI	YGGCCR	1 cut(s)	147
Eam1104I	CTCTTC	2 cut(s)	125, 850
EarI	CTCTTC	2 cut(s)	125, 850
Ecl136II	GAGCTC	1 cut(s)	874
Eco130I	CCWWGG	2 cut(s)	486, 1053
Eco24I	GRGCYC	2 cut(s)	185, 876
Eco47I	GGWCC	3 cut(s)	30, 455, 1061
Eco53kI	GAGCTC	1 cut(s)	874
Eco57I	CTGAAG	2 cut(s)	719, 954
Eco81I	CCTNAGG	1 cut(s)	1038
EcoICRI	GAGCTC	1 cut(s)	874
EcoRI	GAATTC	1 cut(s)	822
EcoRII	CCWGG	3 cut(s)	171, 460, 779
EcoT14I	CCWWGG	2 cut(s)	486, 1053
EcoT38I	GRGCYC	2 cut(s)	185, 876
ErhI	CCWWGG	2 cut(s)	486, 1053
Esp3I	CGTCTC	2 cut(s)	988, 1079
FaeI	CATG	5 cut(s)	224, 391, 611, 683, 802
FaqI	GGGAC	2 cut(s)	226, 478
FatI	CATG	5 cut(s)	220, 387, 607, 679, 798
FbaI	TGATCA	1 cut(s)	214
Fnu4HI	GCNGC	7 cut(s)	65, 68, 559, 765, 795, 978, 986
FokI	GGATG	3 cut(s)	737, 803, 1055
FriOI	GRGCYC	2 cut(s)	185, 876
Fsp4HI	GCNGC	7 cut(s)	65, 68, 559, 765, 795, 978, 986
FspBI	CTAG	1 cut(s)	600
GlaI	GCGC	2 cut(s)	557, 612
GluI	GCNGC	7 cut(s)	65, 68, 559, 765, 795, 978, 986
GsuI	CTGGAG	1 cut(s)	444
HaeIII	GGCC	2 cut(s)	149, 1108
HapII	CCGG	3 cut(s)	59, 117, 805
HgaI	GACGC	4 cut(s)	650, 683, 797, 1005
HhaI	GCGC	2 cut(s)	558, 613
Hin1I	GRCGYC	2 cut(s)	694, 808
Hin1II	CATG	5 cut(s)	224, 391, 611, 683, 802
Hin6I	GCGC	2 cut(s)	556, 611
HinP1I	GCGC	2 cut(s)	556, 611
HincII	GTYRAC	1 cut(s)	656
HindII	GTYRAC	1 cut(s)	656
HindIII	AAGCTT	1 cut(s)	902
HinfI	GANTC	3 cut(s)	204, 260, 563
HpaII	CCGG	3 cut(s)	59, 117, 805
HphI	GGTGA	1 cut(s)	674
Hpy166II	GTNNAC	3 cut(s)	84, 656, 746
Hpy188I	TCNGA	5 cut(s)	7, 568, 699, 952, 993
Hpy188III	TCNNGA	4 cut(s)	117, 320, 812, 1037
Hpy8I	GTNNAC	3 cut(s)	84, 656, 746
Hpy99I	CGWCG	1 cut(s)	11
HpyAV	CCTTC	3 cut(s)	574, 659, 929
HpyCH4III	ACNGT	2 cut(s)	432, 1033
HpyCH4IV	ACGT	1 cut(s)	658
HpyCH4V	TGCA	6 cut(s)	297, 497, 507, 794, 969, 985
HpyF10VI	GCNNNNNNNGC	6 cut(s)	76, 146, 601, 610, 857, 886
HpyF3I	CTNAG	3 cut(s)	565, 992, 1038
HpySE526I	ACGT	1 cut(s)	658
Hsp92I	GRCGYC	2 cut(s)	694, 808
Hsp92II	CATG	5 cut(s)	224, 391, 611, 683, 802
HspAI	GCGC	2 cut(s)	556, 611
Kpn2I	TCCGGA	1 cut(s)	116
Ksp22I	TGATCA	1 cut(s)	214
Kzo9I	GATC	2 cut(s)	214, 1065
LguI	GCTCTTC	1 cut(s)	850
LmnI	GCTCC	6 cut(s)	22, 61, 188, 807, 871, 879
Lsp1109I	GCAGC	5 cut(s)	79, 751, 806, 964, 997
LweI	GCATC	4 cut(s)	348, 542, 613, 781
MaeI	CTAG	1 cut(s)	600
MaeII	ACGT	1 cut(s)	658
MaeIII	GTNAC	1 cut(s)	121
MalI	GATC	2 cut(s)	216, 1067
MboI	GATC	2 cut(s)	214, 1065
MboII	GAAGA	8 cut(s)	142, 712, 748, 767, 867, 1028, 1088, 1108
MhlI	GDGCHC	2 cut(s)	185, 876
MlsI	TGGCCA	1 cut(s)	149
MluCI	AATT	5 cut(s)	161, 306, 636, 645, 822
MluNI	TGGCCA	1 cut(s)	149
MlyI	GAGTC	2 cut(s)	213, 557
MmeI	TCCRAC	2 cut(s)	30, 1039
Mox20I	TGGCCA	1 cut(s)	149
MroI	TCCGGA	1 cut(s)	116
MroXI	GAANNNNTTC	1 cut(s)	112
MscI	TGGCCA	1 cut(s)	149
MseI	TTAA	3 cut(s)	209, 327, 425
MslI	CAYNNNNRTG	2 cut(s)	198, 1134
Msp20I	TGGCCA	1 cut(s)	149
MspA1I	CMGCKG	1 cut(s)	140
MspI	CCGG	3 cut(s)	59, 117, 805
MspR9I	CCNGG	3 cut(s)	173, 462, 781
Mva1269I	GAATGC	1 cut(s)	361
MvaI	CCWGG	3 cut(s)	173, 462, 781
MvnI	CGCG	2 cut(s)	128, 558
MwoI	GCNNNNNNNGC	6 cut(s)	76, 146, 601, 610, 857, 886
NdeII	GATC	2 cut(s)	214, 1065
NlaIII	CATG	5 cut(s)	224, 391, 611, 683, 802
NlaIV	GGNNCC	3 cut(s)	63, 184, 1035
NmuCI	GTSAC	1 cut(s)	121
PasI	CCCWGGG	1 cut(s)	780
PciSI	GCTCTTC	1 cut(s)	850
PctI	GAATGC	1 cut(s)	361
PdmI	GAANNNNTTC	1 cut(s)	112
PfeI	GAWTC	1 cut(s)	260
PflMI	CCANNNNNTGG	1 cut(s)	27
PkrI	GCNGC	7 cut(s)	66, 69, 560, 766, 796, 979, 987
PleI	GAGTC	2 cut(s)	212, 557
PpsI	GAGTC	2 cut(s)	212, 557
Psp124BI	GAGCTC	1 cut(s)	876
Psp6I	CCWGG	3 cut(s)	171, 460, 779
PspGI	CCWGG	3 cut(s)	171, 460, 779
PspN4I	GGNNCC	3 cut(s)	63, 184, 1035
PspPI	GGNCC	3 cut(s)	30, 455, 1061
PstNI	CAGNNNCTG	2 cut(s)	27, 991
PvuII	CAGCTG	1 cut(s)	140
RsaI	GTAC	3 cut(s)	410, 717, 885
RsaNI	GTAC	3 cut(s)	409, 716, 884
RseI	CAYNNNNRTG	2 cut(s)	198, 1134
SacI	GAGCTC	1 cut(s)	876
SapI	GCTCTTC	1 cut(s)	850
SaqAI	TTAA	3 cut(s)	209, 327, 425
SatI	GCNGC	7 cut(s)	65, 68, 559, 765, 795, 978, 986
Sau3AI	GATC	2 cut(s)	214, 1065
Sau96I	GGNCC	3 cut(s)	30, 455, 1061
ScaI	AGTACT	1 cut(s)	410
SchI	GAGTC	2 cut(s)	213, 557
ScrFI	CCNGG	3 cut(s)	173, 462, 781
SduI	GDGCHC	2 cut(s)	185, 876
SfaNI	GCATC	4 cut(s)	348, 542, 613, 781
SinI	GGWCC	3 cut(s)	30, 455, 1061
SmiMI	CAYNNNNRTG	2 cut(s)	198, 1134
SmlI	CTYRAG	1 cut(s)	812
SmoI	CTYRAG	1 cut(s)	812
Sse9I	AATT	5 cut(s)	161, 306, 636, 645, 822
SsiI	CCGC	4 cut(s)	65, 126, 233, 558
SspMI	CTAG	1 cut(s)	600
SstI	GAGCTC	1 cut(s)	876
StyD4I	CCNGG	3 cut(s)	171, 460, 779
StyI	CCWWGG	2 cut(s)	486, 1053
TaaI	ACNGT	2 cut(s)	432, 1033
TaiI	ACGT	1 cut(s)	661
TaqI	TCGA	2 cut(s)	867, 958
TaqII	GACCGA	1 cut(s)	1078
TasI	AATT	5 cut(s)	161, 306, 636, 645, 822
TatI	WGTACW	2 cut(s)	408, 883
TauI	GCSGC	2 cut(s)	67, 561
TfiI	GAWTC	1 cut(s)	260
Tru1I	TTAA	3 cut(s)	209, 327, 425
Tru9I	TTAA	3 cut(s)	209, 327, 425
TscAI	CASTG	1 cut(s)	387
TseFI	GTSAC	1 cut(s)	121
TseI	GCWGC	5 cut(s)	67, 764, 794, 977, 985
Tsp45I	GTSAC	1 cut(s)	121
TspDTI	ATGAA	3 cut(s)	648, 696, 1059
TspGWI	ACGGA	1 cut(s)	175
TspRI	CASTG	1 cut(s)	387
Van91I	CCANNNNNTGG	1 cut(s)	27
VpaK11BI	GGWCC	3 cut(s)	30, 455, 1061
XapI	RAATTY	1 cut(s)	822
XcmI	CCANNNNNNNNNTGG	1 cut(s)	327
XmnI	GAANNNNTTC	1 cut(s)	112
XspI	CTAG	1 cut(s)	600
ZrmI	AGTACT	1 cut(s)	410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.