Rorug05G0210900

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002713
Physical Location & Seq
Reverse (-)
21642772 .. 21643224
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0210900.1

Sequence Viewer

Length: 453 bp
ATGATGAAGTTGATGAACGTCTCCTTTATAGTACTCCTAGCAACAATGGCCATTGTTTCAGCTGAATATCTAGACGATTACAGCAATGAAGAAATGCAAATTCAAAATACCGAATCAACAGCGTCTGAAATGCAAGAAGCAACAACTTCTTTGAGAGGGGTAAGCCGCTTCCTGACCCAGCAGAACCTGTTGGCGAGCGTGACTTGCGATAAGTTACCTAGGGTTTGTCGTCTGAAGAAGAGCCCGGGGCCTGACTGCTGCAAGAAGAAGTGTGTGAATTTGAAGACGGATAGATTGAATTGCGGGATGTGCGGGTACAAGTGCAAGTACACTGAGATTTGCTGCAGGGGGACATGCGTGAATGCATCGTTTGACGAAAGGCATTGCGGTGGATGCCACCAGAAGTGCAAGAAAGGGGAGTTTTGTGTTTTGGGGCTGTGCAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

150

Amino Acids

16.74

Weight (kDa)

8.45

Isoelectric Point (pI)

22.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 67 - 150 1e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 166, 303, 312, 387
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 99, 277
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 3 cut(s) 33, 317, 329
AgsI TTSAA 3 cut(s) 104, 283, 298
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 25
AlwNI CAGNNNCTG 2 cut(s) 125, 187
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 2 cut(s) 48, 248
ApeKI GCWGC 2 cut(s) 258, 342
ApoI RAATTY 2 cut(s) 99, 277
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 2 cut(s) 245, 246
AvaI CYCGRG 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 218
BalI TGGCCA 1 cut(s) 50
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 2 cut(s) 245, 329
BcnI CCSGG 2 cut(s) 245, 246
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 3 cut(s) 38, 71, 219
BfmI CTRYAG 1 cut(s) 343
BisI GCNGC 3 cut(s) 166, 259, 343
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 3 cut(s) 167, 260, 344
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 2 cut(s) 245, 246
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 245, 246
BmsI GCATC 2 cut(s) 374, 383
BpiI GAAGAC 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 245, 246
BsaJI CCNNGG 3 cut(s) 218, 244, 245
Bse3DI GCAATG 2 cut(s) 91, 382
BseDI CCNNGG 3 cut(s) 218, 244, 245
BseGI GGATG 2 cut(s) 312, 398
BseMI GCAATG 2 cut(s) 91, 382
BseMII CTCAG 1 cut(s) 324
BseXI GCAGC 2 cut(s) 245, 329
BseYI CCCAGC 1 cut(s) 177
BshFI GGCC 2 cut(s) 50, 250
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 25
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 50, 250
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 245
BspACI CCGC 4 cut(s) 166, 303, 312, 387
BspANI GGCC 2 cut(s) 50, 250
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 347
BspQI GCTCTTC 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 91, 382
BssECI CCNNGG 3 cut(s) 218, 244, 245
BssT1I CCWWGG 1 cut(s) 218
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 312, 398
BstMAI GTCTC 1 cut(s) 25
BstMWI GCNNNNNNNGC 4 cut(s) 47, 204, 309, 393
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 243, 244
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 245, 329
BstV2I GAAGAC 1 cut(s) 290
BsuRI GGCC 2 cut(s) 50, 250
BtsCI GGATG 2 cut(s) 312, 398
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 2 cut(s) 125, 187
Cfr13I GGNCC 1 cut(s) 248
Cfr9I CCCGGG 1 cut(s) 244
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 32, 316, 328
CviAII CATG 2 cut(s) 354, 450
CviJI RGCY 6 cut(s) 50, 62, 165, 243, 250, 436
CviKI_1 RGCY 6 cut(s) 50, 62, 165, 243, 250, 436
CviQI GTAC 3 cut(s) 32, 316, 328
DdeI CTNAG 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 218
EcoT22I ATGCAT 2 cut(s) 367, 451
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 218
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 2 cut(s) 357, 453
FaiI YATR 4 cut(s) 29, 355, 447, 451
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 353, 449
FauI CCCGC 2 cut(s) 296, 305
Fnu4HI GCNGC 3 cut(s) 166, 259, 343
FokI GGATG 2 cut(s) 319, 405
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 3 cut(s) 166, 259, 343
FspBI CTAG 3 cut(s) 38, 71, 219
GluI GCNGC 3 cut(s) 166, 259, 343
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 50, 250
HapII CCGG 1 cut(s) 245
HgaI GACGC 1 cut(s) 111
Hin1II CATG 2 cut(s) 357, 453
HinfI GANTC 1 cut(s) 113
HpaII CCGG 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 2 cut(s) 127, 234
Hpy188III TCNNGA 2 cut(s) 71, 172
Hpy8I GTNNAC 1 cut(s) 330
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 9 cut(s) 97, 133, 261, 324, 345, 365, 408, 441, 449
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 204, 309, 393
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 2 cut(s) 357, 453
LguI GCTCTTC 1 cut(s) 233
LpnPI CCDG 7 cut(s) 185, 191, 200, 258, 264, 331, 413
Lsp1109I GCAGC 2 cut(s) 245, 329
LweI GCATC 2 cut(s) 374, 383
MaeI CTAG 3 cut(s) 38, 71, 219
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 199, 213
MboII GAAGA 5 cut(s) 101, 247, 250, 277, 295
MhlI GDGCHC 1 cut(s) 245
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 4 cut(s) 99, 277, 298, 442
MluNI TGGCCA 1 cut(s) 50
MnlI CCTC 1 cut(s) 149
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 2 cut(s) 367, 451
MscI TGGCCA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 2 cut(s) 245, 246
Mva1269I GAATGC 1 cut(s) 367
MwoI GCNNNNNNNGC 4 cut(s) 47, 204, 309, 393
NciI CCSGG 2 cut(s) 245, 246
NlaIII CATG 2 cut(s) 357, 453
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 2 cut(s) 367, 451
NspI RCATGY 1 cut(s) 357
PciSI GCTCTTC 1 cut(s) 233
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 3 cut(s) 167, 260, 344
PspFI CCCAGC 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 248
PstI CTGCAG 1 cut(s) 347
PstNI CAGNNNCTG 2 cut(s) 125, 187
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 33, 317, 329
RsaNI GTAC 3 cut(s) 32, 316, 328
RseI CAYNNNNRTG 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 233
SatI GCNGC 3 cut(s) 166, 259, 343
Sau96I GGNCC 1 cut(s) 248
ScaI AGTACT 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 245, 246
SduI GDGCHC 1 cut(s) 245
SetI ASST 4 cut(s) 21, 64, 189, 220
SfaNI GCATC 2 cut(s) 374, 383
SfcI CTRYAG 1 cut(s) 343
SmaI CCCGGG 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 4 cut(s) 99, 277, 298, 442
SsiI CCGC 4 cut(s) 166, 303, 312, 387
SspMI CTAG 3 cut(s) 38, 71, 219
StyD4I CCNGG 2 cut(s) 243, 244
StyI CCWWGG 1 cut(s) 218
TaiI ACGT 1 cut(s) 21
TasI AATT 4 cut(s) 99, 277, 298, 442
TatI WGTACW 2 cut(s) 31, 327
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 258, 342
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 20, 29, 102
TspGWI ACGGA 1 cut(s) 302
TspMI CCCGGG 1 cut(s) 244
TspRI CASTG 1 cut(s) 337
XapI RAATTY 2 cut(s) 99, 277
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 1 cut(s) 357
XmaI CCCGGG 1 cut(s) 244
XmaJI CCTAGG 1 cut(s) 218
XspI CTAG 3 cut(s) 38, 71, 219
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 2 cut(s) 367, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.