Rh5DG312400

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002713
Physical Location & Seq
Reverse (-)
39992411 .. 40001156
8746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG312400.1

Sequence Viewer

Length: 2721 bp
ATGGCAATTATTTTGTCCTTTTCCTGGCAATTCTGGGTTCTAATCTGTAACATTCTTTTATCTTCGGCCAGTATTTCCTCTTCAGACCTGGTTTCAAAGTGGATGGGTGAGAGTGAGAAGCTAGTTTCAAACCTTTTCCAAATGGCTCGAGATAGTGCCCCATCCATCATCTTTGTTGATGAAATAGATTCCTTATGTGGGACGCGTGGAGAAGGCAATGAGAGTGAAGCTTCTAGACGAATCAAGACAGAACTTCTTGTGCAGATGCAGGGTGTGGGAACCAATGACCAAAAAGTTCTTGTTCTTGCAGCGACAAATACTCCCTATGCATTAGACCAGGCCATTCGAAGACGTTTTGACAAGCGTATTTACATTCCCCTTCCAGATGTAAAGGCTCGACAGCATATGTTCAAAGTCCACTTGGGAGATACTCCTCACAACTTGACAGAAAGTGATTTTGAAAGCTTAGCGTTCAAAACTGAGGGATTTTCGGGTTCAGATATTTCTGTTTGTGTCAAGGATGTTCTTTTTGAACCTGTACGCAAAACACAAGATGCCATGTTCTTTTTCAAGAATTCTGATAATATGTTCATACCTTGTGGACCAAAGCAACCGGGTGCGGTCCAAATCACCATGCAGGAGTTGGCAGCTCAAGGACTAGCTTCACAGATCCTTCCACCTCCCATCACAAAAACAGATTTTGATAAGGTACTTGCTAGACAGAGGCCTACTGTGAGCAAAGGGGACCTTGAAGTCCATGAAAGGTTCACGAAAGAATTTGGAGAGGAAGGACTTTCTTTGGCCCATGGAGCCACTGCTGACACAAAGCACTATATTGTTTACATGGGGCACCACTCGCACACCAACTCGGAATCTGTCATCAGAGCTAACCATGAGATTCTTGCTTCAGTCACCGGCAGTACCGGTGGACAAGAAGCAGCACTTAACCATTACAGTAAAAGTTTCCAAGGCTTCTCAGCCAAGCTTACACCAGATCAAGCTCAGCAACTTGCTGAACATGATTTGGTTATTTCGGTGTTTGAGAGCAAGACAAACAAACTGAGCACAACACATTCCTGGGATTTTCTAGGCGTAGACGCTACTCCTAATTACAATCAAATGCCAATGGGTTCCAAATCCAATGTCATTGTTGGTGTAATTGACAGTGGAGTTTGGCCAGAGTCAGAGAGCTTCAATGATGAAGGATTAGGACCTGTGCCAGCTAAATTCAGAGGAGAGTGTGTTACTGGTCAGAATTTTACATTGGCCAACTGCAACAGAAAAATCATAGGCTCTCGCTTCTATTATCATGGATTTGAAGTAGAAAATGGGCCACTTGAGTCCTTTGCACCTCTTCCTTTCTTCAGATCAGCTCGAGATGCTGATGGACATGGGAGGAACTCTGGTTTCCCCAGTACTGCATGCAATGTAGCTCCCTGGATTCTCACTATTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTTTATCTGGGAAATTCACAAATACTAAAGGGTTCCTCTTTAAACCCACTAAAGATGGATAAGTCTTACAGTTTGATTGCTGCAAGTGTTGCAGCTGCCCCTGGAGTCACAGCAAAGAATGCAAGCTTCTGCAAGGACAAAACTCTAAATGCAAGCTTAATTAAGGGAAAAATCGTTGTGTGCACCTTTGAGACATTTACCGACAACCGAAGGGAAAAAAGTATAGTTGTAAGACAAGGTGGTGGTGTAGGAATGATTCTTGTGGATCCATTTCTCAAAGATGTTGGCTTTGAGTTTGTCATCCCGGCCACCCTAATTGGTCAAGAAGAAGCAGCACAGCTTCAAGCATACATGACAACAGAGAAGTACCCAGTTGCTAGAATCTCACAAACGACAACAATTCTGAAAACTAAACCTGCACCTGAGATGGCAGTGTTCTCCTCCATGGGGCCAAATATCATAACCCCAGACATTATCAAACCAGATATTACAGGACCTGGGGTGAATATCTTAGCAGCATGGTCTCCGGTCTCAACTACACCCACAGCAGAAAGGTCTGTCAATTATAACATCATTTCTGGCACCTCGATGTCTTGTCCACATGTATCTGCAGTTGCAGCAACTTTGAAATCATACCGACCTTCCTGGAGTCCAGCAGCCATAATGTCTGCAATAATGACAACATCAACAACTCTAGACAACAACAAACGCCCTATTGGGAGAGATCCAAATGGCACCCCTACCACACCTTTTGACTACGGATCTGGACACATTAACCCAGTTGCAGCAATTGATCCTGGACTTGTATATGATTTTGATTCCCATGACATAATCGACTTTCTCTGCAGCACTGGGGCTAGCACTCTGCAACTGAAAAACCTCACGGGTAATCTAGTTTATTGCCAGAAGCCTTCTATACCTTCCTACAATTTTAACTACCCTTCCATTGGCGTGTCAAAAATGAGTGGAAGGCTATCTGTTCAGCGTACAGTTACTTATTACGGCAAAGGCCCAACGGTATATGCTGCATATGTAGACTACCCAGCAGGTGTAAATGTTACAGTAACACCAGCTAAGCTCAAGTTTACAAATACCGGGGAAAAGATGTCTTTCTGTGTTGATTTCACCCCCCTCAAGAATAGTAATGGAAGCTTTGTGTTTGGAGCTCTGACATGGAGTAATGGTATTCAGAAGGTTAGGAGTCCTATTGGTCTTAATGTGCTTTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

906

Amino Acids

98.5

Weight (kDa)

6.84

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 23 - 127 5.7e-28 ATPase family associated with various cellular activities (AAA)
Vps4_C PF09336 198 - 261 1.8e-21 Vps4 C terminal oligomerisation domain
Inhibitor_I9 PF05922 278 - 354 5.2e-15 Peptidase inhibitor I9
PA PF02225 534 - 610 3.3e-07 PA domain
Peptidase_S8 PF00082 625 - 732 1.6e-18 Subtilase family
fn3_6 PF17766 806 - 899 4.8e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2055
AarI CACCTGC 1 cut(s) 2525
AasI GACNNNNNNGTC 1 cut(s) 752
Acc36I ACCTGC 2 cut(s) 1912, 2525
AccB1I GGYRCC 3 cut(s) 849, 2069, 2222
AccI GTMKAC 2 cut(s) 1095, 2523
AccII CGCG 1 cut(s) 205
AciI CCGC 1 cut(s) 620
AclI AACGTT 1 cut(s) 1488
AclWI GGATC 6 cut(s) 664, 1748, 1761, 2207, 2257, 2276
AcoI YGGCCR 4 cut(s) 66, 1175, 1266, 1794
AcsI RAATTY 6 cut(s) 574, 776, 1226, 1255, 1475, 1501
AcuI CTGAAG 3 cut(s) 66, 891, 1348
AfaI GTAC 6 cut(s) 540, 711, 922, 1417, 1856, 2476
AfiI CCNNNNNNNGG 4 cut(s) 24, 198, 1935, 2435
AflIII ACRYGT 2 cut(s) 203, 2089
AgeI ACCGGT 1 cut(s) 923
AjnI CCWGG 9 cut(s) 23, 87, 336, 1076, 1436, 1588, 1984, 2132, 2284
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
Alw21I GWGCWC 3 cut(s) 1067, 1673, 2656
Alw26I GTCTC 3 cut(s) 1673, 2016, 2023
Alw44I GTGCAC 1 cut(s) 1669
AlwI GGATC 6 cut(s) 664, 1748, 1761, 2207, 2257, 2276
AlwNI CAGNNNCTG 1 cut(s) 1985
Ama87I CYCGRG 2 cut(s) 147, 1374
ApaLI GTGCAC 1 cut(s) 1669
ApoI RAATTY 6 cut(s) 574, 776, 1226, 1255, 1475, 1501
ArsI GACNNNNNNTTYG 2 cut(s) 1128, 1160
AsiGI ACCGGT 1 cut(s) 923
AspS9I GGNCC 9 cut(s) 602, 622, 745, 802, 1211, 1331, 1937, 1982, 2498
AsuC2I CCSGG 3 cut(s) 615, 1793, 2584
AsuHPI GGTGA 5 cut(s) 119, 622, 904, 2002, 2605
AsuII TTCGAA 1 cut(s) 346
AsuNHI GCTAGC 1 cut(s) 2345
AvaI CYCGRG 2 cut(s) 147, 1374
AvaII GGWCC 5 cut(s) 602, 622, 745, 1211, 1982
BaeGI GKGCMC 3 cut(s) 160, 852, 1673
BalI TGGCCA 2 cut(s) 1177, 1268
BamHI GGATCC 1 cut(s) 1753
BanI GGYRCC 3 cut(s) 849, 2069, 2222
BanII GRGCYC 1 cut(s) 2656
BarI GAAGNNNNNNTAC 2 cut(s) 64, 96
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 3 cut(s) 1067, 1673, 2656
BccI CCATC 7 cut(s) 97, 169, 173, 692, 1379, 1537, 1909
BceAI ACGGC 1 cut(s) 2506
BciT130I CCWGG 9 cut(s) 25, 89, 338, 1078, 1438, 1590, 1986, 2134, 2286
BcnI CCSGG 3 cut(s) 615, 1793, 2584
BcoDI GTCTC 3 cut(s) 1673, 2016, 2023
BfaI CTAG 9 cut(s) 122, 234, 659, 717, 1088, 1866, 2183, 2346, 2381
BfmI CTRYAG 2 cut(s) 2097, 2332
BfuAI ACCTGC 2 cut(s) 1912, 2525
BlpI GCTNAGC 3 cut(s) 466, 1002, 2562
BmcAI AGTACT 1 cut(s) 1417
Bme18I GGWCC 5 cut(s) 602, 622, 745, 1211, 1982
BmeT110I CYCGRG 2 cut(s) 147, 1374
BmgT120I GGNCC 9 cut(s) 602, 622, 745, 802, 1211, 1331, 1937, 1982, 2498
BmrI ACTGGG 4 cut(s) 1407, 1853, 2261, 2349
BmsI GCATC 3 cut(s) 255, 544, 1369
BmtI GCTAGC 1 cut(s) 2349
BmuI ACTGGG 4 cut(s) 1407, 1853, 2261, 2349
BpiI GAAGAC 1 cut(s) 355
BpmI CTGGAG 2 cut(s) 1611, 2155
Bpu1102I GCTNAGC 3 cut(s) 466, 1002, 2562
Bpu14I TTCGAA 1 cut(s) 346
BpuEI CTTGAG 4 cut(s) 636, 1358, 2552, 2606
BpuMI CCSGG 3 cut(s) 615, 1793, 2584
BsaI GGTCTC 2 cut(s) 2016, 2023
BsaJI CCNNGG 8 cut(s) 805, 967, 1077, 1436, 1588, 1932, 1985, 2583
BsaWI WCCGGW 2 cut(s) 923, 2014
BsaXI ACNNNNNCTCC 6 cut(s) 304, 334, 1227, 1257, 2656, 2686
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 198, 1935, 2435
Bse118I RCCGGY 2 cut(s) 914, 923
Bse1I ACTGG 6 cut(s) 69, 1252, 1413, 1859, 2267, 2344
Bse3DI GCAATG 2 cut(s) 223, 1432
BseBI CCWGG 9 cut(s) 25, 89, 338, 1078, 1438, 1590, 1986, 2134, 2286
BseDI CCNNGG 8 cut(s) 805, 967, 1077, 1436, 1588, 1932, 1985, 2583
BseGI GGATG 4 cut(s) 108, 161, 526, 1788
BseLI CCNNNNNNNGG 4 cut(s) 24, 198, 1935, 2435
BseMI GCAATG 2 cut(s) 223, 1432
BseMII CTCAG 5 cut(s) 471, 990, 1016, 1052, 1902
BseNI ACTGG 6 cut(s) 69, 1252, 1413, 1859, 2267, 2344
BseRI GAGGAG 3 cut(s) 423, 1248, 1918
BseSI GKGCMC 3 cut(s) 160, 852, 1673
BseYI CCCAGC 1 cut(s) 2530
BsgI GTGCAG 2 cut(s) 281, 1890
Bsh1236I CGCG 1 cut(s) 205
BshNI GGYRCC 3 cut(s) 849, 2069, 2222
BshTI ACCGGT 1 cut(s) 923
BsiHKAI GWGCWC 3 cut(s) 1067, 1673, 2656
BsiHKCI CYCGRG 2 cut(s) 147, 1374
BsiSI CCGG 6 cut(s) 614, 915, 924, 1793, 2015, 2583
BslFI GGGAC 2 cut(s) 214, 758
BslI CCNNNNNNNGG 4 cut(s) 24, 198, 1935, 2435
BsmAI GTCTC 3 cut(s) 1673, 2016, 2023
BsmFI GGGAC 2 cut(s) 214, 758
BsmI GAATGC 1 cut(s) 1612
Bso31I GGTCTC 2 cut(s) 2016, 2023
BsoBI CYCGRG 2 cut(s) 147, 1374
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 5 cut(s) 160, 852, 1067, 1673, 2656
Bsp143I GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
Bsp1720I GCTNAGC 3 cut(s) 466, 1002, 2562
Bsp19I CCATGG 2 cut(s) 805, 1932
BspACI CCGC 1 cut(s) 620
BspCNI CTCAG 5 cut(s) 472, 989, 1015, 1053, 1903
BspFNI CGCG 1 cut(s) 205
BspMAI CTGCAG 2 cut(s) 2101, 2336
BspMI ACCTGC 2 cut(s) 1912, 2525
BspOI GCTAGC 1 cut(s) 2349
BspPI GGATC 6 cut(s) 664, 1748, 1761, 2207, 2257, 2276
BspT104I TTCGAA 1 cut(s) 346
BspT107I GGYRCC 3 cut(s) 849, 2069, 2222
BspTNI GGTCTC 2 cut(s) 2016, 2023
BsrDI GCAATG 2 cut(s) 223, 1432
BsrFI RCCGGY 2 cut(s) 914, 923
BsrI ACTGG 6 cut(s) 69, 1252, 1413, 1859, 2267, 2344
BssAI RCCGGY 2 cut(s) 914, 923
BssECI CCNNGG 8 cut(s) 805, 967, 1077, 1436, 1588, 1932, 1985, 2583
BssMI GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
BssT1I CCWWGG 3 cut(s) 805, 967, 1932
Bst2UI CCWGG 9 cut(s) 25, 89, 338, 1078, 1438, 1590, 1986, 2134, 2286
Bst4CI ACNGT 7 cut(s) 733, 956, 1166, 1559, 2479, 2506, 2551
Bst6I CTCTTC 2 cut(s) 85, 1359
BstAPI GCANNNNNTGC 2 cut(s) 1577, 1607
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 6 cut(s) 1221, 1423, 1459, 1612, 1642, 2347
BstDEI CTNAG 8 cut(s) 466, 480, 976, 1002, 1061, 1911, 1999, 2562
BstDSI CCRYGG 2 cut(s) 805, 1932
BstF5I GGATG 4 cut(s) 108, 161, 526, 1788
BstFNI CGCG 1 cut(s) 205
BstKTI GATC 7 cut(s) 672, 997, 1370, 1756, 2215, 2252, 2284
BstMAI GTCTC 3 cut(s) 1673, 2016, 2023
BstMBI GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
BstMWI GCNNNNNNNGC 6 cut(s) 809, 856, 1379, 1577, 1607, 2105
BstNI CCWGG 9 cut(s) 25, 89, 338, 1078, 1438, 1590, 1986, 2134, 2286
BstNSI RCATGY 2 cut(s) 1425, 2093
BstSFI CTRYAG 2 cut(s) 2097, 2332
BstSLI GKGCMC 3 cut(s) 160, 852, 1673
BstUI CGCG 1 cut(s) 205
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 4 cut(s) 669, 1753, 2212, 2249
BstYI RGATCY 4 cut(s) 669, 1753, 2212, 2249
BtgI CCRYGG 2 cut(s) 805, 1932
BtsCI GGATG 4 cut(s) 108, 161, 526, 1788
BtsI GCAGTG 2 cut(s) 813, 1926
BtsIMutI CAGTG 4 cut(s) 813, 1171, 1926, 2337
BveI ACCTGC 2 cut(s) 1912, 2525
Cac8I GCNNGC 6 cut(s) 1221, 1423, 1459, 1612, 1642, 2347
CaiI CAGNNNCTG 1 cut(s) 1985
Cfr10I RCCGGY 2 cut(s) 914, 923
Cfr13I GGNCC 9 cut(s) 602, 622, 745, 802, 1211, 1331, 1937, 1982, 2498
CseI GACGC 2 cut(s) 211, 1106
CsiI ACCWGGT 1 cut(s) 87
Csp6I GTAC 6 cut(s) 539, 710, 921, 1416, 1855, 2475
CspAI ACCGGT 1 cut(s) 923
CspCI CAANNNNNGTGG 4 cut(s) 1786, 1821, 2221, 2256
CviQI GTAC 6 cut(s) 539, 710, 921, 1416, 1855, 2475
DdeI CTNAG 8 cut(s) 466, 480, 976, 1002, 1061, 1911, 1999, 2562
DpnI GATC 7 cut(s) 671, 996, 1369, 1755, 2214, 2251, 2283
DpnII GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
DraI TTTAAA 1 cut(s) 1530
DrdI GACNNNNNNGTC 1 cut(s) 752
DseDI GACNNNNNNGTC 1 cut(s) 752
EaeI YGGCCR 4 cut(s) 66, 1175, 1266, 1794
Eam1104I CTCTTC 2 cut(s) 85, 1359
EarI CTCTTC 2 cut(s) 85, 1359
Ecl136II GAGCTC 1 cut(s) 2654
Eco130I CCWWGG 3 cut(s) 805, 967, 1932
Eco147I AGGCCT 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 2656
Eco31I GGTCTC 2 cut(s) 2016, 2023
Eco47I GGWCC 5 cut(s) 602, 622, 745, 1211, 1982
Eco53kI GAGCTC 1 cut(s) 2654
Eco57I CTGAAG 3 cut(s) 66, 891, 1348
Eco88I CYCGRG 2 cut(s) 147, 1374
EcoICRI GAGCTC 1 cut(s) 2654
EcoO109I RGGNCCY 3 cut(s) 745, 1211, 1982
EcoRI GAATTC 2 cut(s) 574, 1475
EcoRII CCWGG 9 cut(s) 23, 87, 336, 1076, 1436, 1588, 1984, 2132, 2284
EcoT14I CCWWGG 3 cut(s) 805, 967, 1932
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 2656
ErhI CCWWGG 3 cut(s) 805, 967, 1932
FalI AAGNNNNNCTT 4 cut(s) 732, 764, 927, 959
FaqI GGGAC 2 cut(s) 214, 758
FauNDI CATATG 2 cut(s) 405, 2518
FblI GTMKAC 2 cut(s) 1095, 2523
FokI GGATG 4 cut(s) 115, 148, 533, 1775
FriOI GRGCYC 1 cut(s) 2656
FspBI CTAG 9 cut(s) 122, 234, 659, 717, 1088, 1866, 2183, 2346, 2381
GsaI CCCAGC 1 cut(s) 2534
GsuI CTGGAG 2 cut(s) 1611, 2155
HapII CCGG 6 cut(s) 614, 915, 924, 1793, 2015, 2583
HgaI GACGC 2 cut(s) 211, 1106
HindIII AAGCTT 6 cut(s) 228, 463, 983, 1612, 1642, 2638
HpaII CCGG 6 cut(s) 614, 915, 924, 1793, 2015, 2583
HphI GGTGA 5 cut(s) 119, 622, 904, 2002, 2605
HpyCH4III ACNGT 7 cut(s) 733, 956, 1166, 1559, 2479, 2506, 2551
HpyCH4IV ACGT 2 cut(s) 352, 1488
HpyF10VI GCNNNNNNNGC 6 cut(s) 809, 856, 1379, 1577, 1607, 2105
HpyF3I CTNAG 8 cut(s) 466, 480, 976, 1002, 1061, 1911, 1999, 2562
HpySE526I ACGT 2 cut(s) 352, 1488
Kzo9I GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
LmnI GCTCC 3 cut(s) 809, 1438, 2651
LweI GCATC 3 cut(s) 255, 544, 1369
MabI ACCWGGT 1 cut(s) 87
MaeI CTAG 9 cut(s) 122, 234, 659, 717, 1088, 1866, 2183, 2346, 2381
MaeII ACGT 2 cut(s) 352, 1488
MaeIII GTNAC 7 cut(s) 47, 910, 1243, 1594, 2479, 2545, 2551
MalI GATC 7 cut(s) 671, 996, 1369, 1755, 2214, 2251, 2283
MboI GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
MboII GAAGA 6 cut(s) 54, 72, 360, 1346, 1354, 1826
MfeI CAATTG 1 cut(s) 2277
MflI RGATCY 4 cut(s) 669, 1753, 2212, 2249
MhlI GDGCHC 5 cut(s) 160, 852, 1067, 1673, 2656
MlsI TGGCCA 2 cut(s) 1177, 1268
MluI ACGCGT 1 cut(s) 203
MluNI TGGCCA 2 cut(s) 1177, 1268
MlyI GAGTC 5 cut(s) 1190, 1349, 1602, 2146, 2698
Mox20I TGGCCA 2 cut(s) 1177, 1268
Mph1103I ATGCAT 1 cut(s) 331
MscI TGGCCA 2 cut(s) 1177, 1268
MseI TTAA 7 cut(s) 945, 1529, 1646, 1650, 2262, 2421, 2703
MslI CAYNNNNRTG 2 cut(s) 2075, 2438
Msp20I TGGCCA 2 cut(s) 1177, 1268
MspA1I CMGCKG 1 cut(s) 1583
MspI CCGG 6 cut(s) 614, 915, 924, 1793, 2015, 2583
MunI CAATTG 1 cut(s) 2277
Mva1269I GAATGC 1 cut(s) 1612
MvaI CCWGG 9 cut(s) 25, 89, 338, 1078, 1438, 1590, 1986, 2134, 2286
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 6 cut(s) 809, 856, 1379, 1577, 1607, 2105
NciI CCSGG 3 cut(s) 615, 1793, 2584
NcoI CCATGG 2 cut(s) 805, 1932
NdeI CATATG 2 cut(s) 405, 2518
NdeII GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
NheI GCTAGC 1 cut(s) 2345
NmuCI GTSAC 2 cut(s) 910, 1594
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 2 cut(s) 1425, 2093
NspV TTCGAA 1 cut(s) 346
PacI TTAATTAA 1 cut(s) 1650
PaeI GCATGC 1 cut(s) 1425
PaeR7I CTCGAG 2 cut(s) 147, 1374
PaqCI CACCTGC 1 cut(s) 2525
PceI AGGCCT 1 cut(s) 727
PciI ACATGT 1 cut(s) 2089
PctI GAATGC 1 cut(s) 1612
PfeI GAWTC 8 cut(s) 188, 240, 872, 898, 1441, 1744, 1869, 2306
PfoI TCCNGGA 2 cut(s) 2132, 2284
PinAI ACCGGT 1 cut(s) 923
PleI GAGTC 5 cut(s) 1189, 1348, 1601, 2145, 2697
PpsI GAGTC 5 cut(s) 1189, 1348, 1601, 2145, 2697
PpuMI RGGWCCY 3 cut(s) 745, 1211, 1982
PscI ACATGT 1 cut(s) 2089
PsiI TTATAA 1 cut(s) 2055
Psp124BI GAGCTC 1 cut(s) 2656
Psp1406I AACGTT 1 cut(s) 1488
Psp5II RGGWCCY 3 cut(s) 745, 1211, 1982
Psp6I CCWGG 9 cut(s) 23, 87, 336, 1076, 1436, 1588, 1984, 2132, 2284
PspFI CCCAGC 1 cut(s) 2530
PspGI CCWGG 9 cut(s) 23, 87, 336, 1076, 1436, 1588, 1984, 2132, 2284
PspPI GGNCC 9 cut(s) 602, 622, 745, 802, 1211, 1331, 1937, 1982, 2498
PspPPI RGGWCCY 3 cut(s) 745, 1211, 1982
PstI CTGCAG 2 cut(s) 2101, 2336
PstNI CAGNNNCTG 1 cut(s) 1985
PsuI RGATCY 4 cut(s) 669, 1753, 2212, 2249
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 6 cut(s) 540, 711, 922, 1417, 1856, 2476
RsaNI GTAC 6 cut(s) 539, 710, 921, 1416, 1855, 2475
RseI CAYNNNNRTG 2 cut(s) 2075, 2438
SacI GAGCTC 1 cut(s) 2656
SaqAI TTAA 7 cut(s) 945, 1529, 1646, 1650, 2262, 2421, 2703
Sau3AI GATC 7 cut(s) 669, 994, 1367, 1753, 2212, 2249, 2281
Sau96I GGNCC 9 cut(s) 602, 622, 745, 802, 1211, 1331, 1937, 1982, 2498
ScaI AGTACT 1 cut(s) 1417
SchI GAGTC 5 cut(s) 1190, 1349, 1602, 2146, 2698
SduI GDGCHC 5 cut(s) 160, 852, 1067, 1673, 2656
SexAI ACCWGGT 1 cut(s) 87
SfaNI GCATC 3 cut(s) 255, 544, 1369
SfcI CTRYAG 2 cut(s) 2097, 2332
Sfr274I CTCGAG 2 cut(s) 147, 1374
SfuI TTCGAA 1 cut(s) 346
SinI GGWCC 5 cut(s) 602, 622, 745, 1211, 1982
SlaI CTCGAG 2 cut(s) 147, 1374
SmiMI CAYNNNNRTG 2 cut(s) 2075, 2438
SmlI CTYRAG 6 cut(s) 147, 651, 1337, 1374, 2567, 2621
SmoI CTYRAG 6 cut(s) 147, 651, 1337, 1374, 2567, 2621
SphI GCATGC 1 cut(s) 1425
SseBI AGGCCT 1 cut(s) 727
SsiI CCGC 1 cut(s) 620
SspMI CTAG 9 cut(s) 122, 234, 659, 717, 1088, 1866, 2183, 2346, 2381
SstI GAGCTC 1 cut(s) 2656
StuI AGGCCT 1 cut(s) 727
StyI CCWWGG 3 cut(s) 805, 967, 1932
TaaI ACNGT 7 cut(s) 733, 956, 1166, 1559, 2479, 2506, 2551
TaiI ACGT 2 cut(s) 355, 1491
TaqI TCGA 6 cut(s) 148, 346, 397, 1375, 2075, 2322
TatI WGTACW 1 cut(s) 1415
TfiI GAWTC 8 cut(s) 188, 240, 872, 898, 1441, 1744, 1869, 2306
Tru1I TTAA 7 cut(s) 945, 1529, 1646, 1650, 2262, 2421, 2703
Tru9I TTAA 7 cut(s) 945, 1529, 1646, 1650, 2262, 2421, 2703
TscAI CASTG 4 cut(s) 820, 1171, 1926, 2344
TseFI GTSAC 2 cut(s) 910, 1594
Tsp45I GTSAC 2 cut(s) 910, 1594
TspDTI ATGAA 4 cut(s) 195, 580, 774, 1215
TspGWI ACGGA 1 cut(s) 2262
TspRI CASTG 4 cut(s) 820, 1171, 1926, 2344
VneI GTGCAC 1 cut(s) 1669
VpaK11BI GGWCC 5 cut(s) 602, 622, 745, 1211, 1982
XapI RAATTY 6 cut(s) 574, 776, 1226, 1255, 1475, 1501
XbaI TCTAGA 2 cut(s) 233, 2182
XceI RCATGY 2 cut(s) 1425, 2093
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XhoI CTCGAG 2 cut(s) 147, 1374
XmiI GTMKAC 2 cut(s) 1095, 2523
XspI CTAG 9 cut(s) 122, 234, 659, 717, 1088, 1866, 2183, 2346, 2381
ZrmI AGTACT 1 cut(s) 1417
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.