Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0002713

Physical Location & Seq

Genomic Coordinates Reverse (-)

41189696 .. 41194191

Gene Structure 4496 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG295800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGGTTTCTGGTTATATCCTGAAACTTACTATTGTCCTCACCCTTCTCTTGTTCCTTAGACTTTCTTTGGCCCATGGTGCCACTGCTGACACAAAGCACTATATTGTTTACATGGGGCACCACTCGCACACCAACTCGGAATCTGTCATCAGAGCTAACCATGAGATTCTTGCTTCAGTCACCGGCAGTACCGGTGGACAAGAAGCAGCACTTAACCATTATAGTAAAAGTTTCCAAGGCTTCTCAGCCAAGCTTACACCAGATCAAGCTCAGCAACTTGCTGAACATGATTCGGTTATTTCGGTGTTTGAGAGCAAGACAAACAAACTGAGCACAACACATTCCTGGGATTTTCTAGGCGTAGACGCTACTCCTAATTACAATCAAATGCCAATGGGTTCCAAATCCAATGTCATTGTTGGTGTAATTGACAGTGGAGTTTGGCCAGAGTCAGAGAGCTTCAATGATGAAGGATTAGGACCTGTGCCAGCTAAATTCAGAGGAGAGTGTGTTACTGGTCAGAATTTTACATTGGCCAACTGCAACAGAAAAATCATAGGCTCTCGCTTCTATTATCATGGATTTGAAGTAGAAAATGGGCCACTTGAGTCCTTTGCACCTCTTCCTTTCTTCAGATCAGCTCGAGATGCTGATGGACATGGGAGTCACACTGCCTCCACGATAGCTGGATCTACAGTAACTAATGTCAGCTTCTTGGGAATGGCCGCAGGAACTGCAAGAGGCGGTGCAGAAAGTGCCAGACTTGCTATCTACAAGGCATGTTGGTTCGGCTTGTGTAGTGATGCAGACCTTCTTTCAGCTCTGGATGATGCCATTGGCGATGGTGTCGATATTCTGTCCCTTTCCCTTGGCCCTGATCCTCCACAGCCAACATATTTTGAAGATGCAGTCTCCATTGGAGCTTTCCATGCATTCCAGAAAGGAATACTTGTTTCTGCATCAGCAGGGAACTCTGGTTTCCCCAGTACTGCATGCAATGTAGCTCCCTGGATTCTCACTGTTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTTTATCTGGGAAATTCACAAATACTAAAGGGTTCCTCTTTAAACCCACTGAAGATGGATAAGTCTTACAGTTTGATTGCTGCAAGTGTTGCAGCTGCCCCTGGAGTCACAGCAAAGAATGCAAGCTTCTGCAAGGACAATACTCTAAATGCAAGCTTAATTAAGGGAAAAATCGTTGTGTGCACCTTTGAGACATTTACCGACAACCGAATGGAAAAAAGTATAGTTGTAAGACAAGGTGGTGGTGTAGGAATGATTCTTGTGGATCCATTTCTCAAAGATGTTGGCTTTGAGTTTGTCATCCCGGCCACCCTAATTGGTCAAGAAGAAGCAGCACAGCTTCAAGCATACATGACAACAGAGAAGTACCCAGTTGCTAGAATATCACAAACGACAACAATTCTGAAAACTAAACCTGCACCTGAGATGGCAGTGTTCTCCTCCATGGGGCCAAATATCATAACCCCAGACATTATCAAACCAGATATTACAGGACCTGGGGTGAATATCTTAGCAGCATGGTCTCCGGTCTCAACTACACCCACAGCAGAAAGGTCTGTCAATTATAACATCATTTCTGGCACCTCGATGTCTTGTCCACATGTATCTGCAGTTGCAGTAACTTTGAAATCATACCGACCTTCCTGGAGTCCAGCAGCCATAATGTCTGCAATAATGACAACATCAACAACTCTAGACAACAACAAGCGCCCTATTGGGAGAGATCCGAATGGCACCCCTACCACACCTTTTGACTACGGATCTGGACACATTAACCCAGTTGCAGCAATTGATCCTGGACTTGTATATGATTTTGATTCCCATGACATAATCGACTTTCTCTGCAGCACTGGGGCTAGCACTCTGCAACTGAAAAACCTCACGGGTAATCTAGTTTATTGCCAGAAGCCTTCTATACCTTCCTACAATTTTAACTACCCTTCCATTGGCGTGTCAAAAATGAGTGGAAGGCTATCTGTTCAGCGTACAGTTACTTATTACGGCAAAGGCCCAACGGTATATGCTGCATATGTAGACTACCCAGCAGGTGTAAATGTTACAGTAACACCAGCTAAACTCAAGTTTACAAATACCGGGGAAAAGATGTCTTTCTGTGTTGATTTCACCCCCCTCAAGAATAGTAATGGAAGCTTTGTGTTTGGAGCTCTGACATGGAGTAATGGTATTCAGAATGTTAGGAGTCCTATTGGTCTTAATGTGGTTTCAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

763

Amino Acids

81.29

Weight (kDa)

6.39

Isoelectric Point (pI)

32.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 110	1.6e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 589	2.4e-41	Subtilase family
PA	PF02225	391 - 467	3.2e-07	PA domain
fn3_6	PF17766	663 - 756	4.6e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002713)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34560 AT2G34560
fragaria_vesca	FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g05520 FvH4_3g24600
malus_domestica	MD05G1283700.v1.1 MD10G1261900.v1.1 MD11G1161400.v1.1 MD17G1012100.v1.1
prunus_persica	Prupe.4G080800_v2.0.a1 Prupe.4G080800_v2.0.a1 Prupe.6G127100_v2.0.a1
pyrus_communis	pycom05g26280 pycom07g21360 pycom10g21820 pycom11g13380
rosa_chinensis	RchiOBHm_Chr5g0014221 RchiOBHm_Chr5g0044151
rosa_laevigata	RLG00000032091 RLG00000034247
rosa_multiflora	Rmu_sc0000939.1_g000032 Rmu_sc0014980.1_g000003 Rmu_ssc0000120.1_g000006
rosa_roxburghii	Rroxscaffold_1G00036900 Rroxscaffold_1G00062360
rosa_rugosa	Rorug05G0016300 Rorug05G0210900 Rorug05G0211000
rosa_samantha	Rh5AG295800 Rh5BG303600 Rh5CG119300 Rh5CG330600 Rh5DG312400
rosa_wichuraiana	Rw5G009640 Rw5G027350 Rw5G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1626
AarI	CACCTGC	1 cut(s)	2096
AasI	GACNNNNNNGTC	1 cut(s)	663
Acc36I	ACCTGC	2 cut(s)	1483, 2096
AccB1I	GGYRCC	4 cut(s)	77, 117, 1640, 1793
AccI	GTMKAC	2 cut(s)	363, 2094
AciI	CCGC	2 cut(s)	726, 744
AclI	AACGTT	1 cut(s)	1059
AclWI	GGATC	7 cut(s)	697, 872, 1319, 1332, 1778, 1828, 1847
AcoI	YGGCCR	4 cut(s)	443, 534, 723, 1365
AcsI	RAATTY	4 cut(s)	494, 523, 1046, 1072
AcuI	CTGAAG	3 cut(s)	159, 616, 1130
AfaI	GTAC	4 cut(s)	190, 988, 1427, 2047
AfiI	CCNNNNNNNGG	2 cut(s)	1506, 2006
AflIII	ACRYGT	1 cut(s)	1660
AgeI	ACCGGT	1 cut(s)	191
AgsI	TTSAA	7 cut(s)	463, 587, 902, 1056, 1403, 1687, 2286
AjnI	CCWGG	6 cut(s)	344, 1007, 1159, 1555, 1703, 1855
AjuI	GAANNNNNNNTTGG	2 cut(s)	515, 547
Alw21I	GWGCWC	3 cut(s)	335, 1244, 2227
Alw26I	GTCTC	4 cut(s)	916, 1244, 1587, 1594
Alw44I	GTGCAC	1 cut(s)	1240
AlwI	GGATC	7 cut(s)	697, 872, 1319, 1332, 1778, 1828, 1847
AlwNI	CAGNNNCTG	2 cut(s)	734, 1556
Ama87I	CYCGRG	1 cut(s)	642
AoxI	GGCC	9 cut(s)	69, 443, 534, 599, 723, 871, 1365, 1508, 2068
ApaLI	GTGCAC	1 cut(s)	1240
ApoI	RAATTY	4 cut(s)	494, 523, 1046, 1072
ArsI	GACNNNNNNTTYG	2 cut(s)	396, 428
AsiGI	ACCGGT	1 cut(s)	191
AspLEI	GCGC	1 cut(s)	1770
AspS9I	GGNCC	7 cut(s)	70, 479, 599, 872, 1508, 1553, 2069
AsuC2I	CCSGG	2 cut(s)	1364, 2155
AsuHPI	GGTGA	4 cut(s)	31, 172, 1573, 2176
AsuNHI	GCTAGC	1 cut(s)	1916
AvaI	CYCGRG	1 cut(s)	642
AvaII	GGWCC	2 cut(s)	479, 1553
BaeGI	GKGCMC	2 cut(s)	120, 1244
BalI	TGGCCA	2 cut(s)	445, 536
BamHI	GGATCC	1 cut(s)	1324
BanI	GGYRCC	4 cut(s)	77, 117, 1640, 1793
BanII	GRGCYC	1 cut(s)	2227
Bbv12I	GWGCWC	3 cut(s)	335, 1244, 2227
BccI	CCATC	4 cut(s)	647, 836, 1108, 1480
BceAI	ACGGC	1 cut(s)	2077
BciT130I	CCWGG	6 cut(s)	346, 1009, 1161, 1557, 1705, 1857
BcnI	CCSGG	2 cut(s)	1364, 2155
BcoDI	GTCTC	4 cut(s)	916, 1244, 1587, 1594
BfaI	CTAG	5 cut(s)	356, 1437, 1754, 1917, 1952
BfmI	CTRYAG	3 cut(s)	693, 1668, 1903
BfoI	RGCGCY	1 cut(s)	1771
BfuAI	ACCTGC	2 cut(s)	1483, 2096
BlpI	GCTNAGC	1 cut(s)	270
BmcAI	AGTACT	1 cut(s)	988
Bme1390I	CCNGG	8 cut(s)	346, 1009, 1161, 1364, 1557, 1705, 1857, 2155
Bme18I	GGWCC	2 cut(s)	479, 1553
BmeT110I	CYCGRG	1 cut(s)	642
BmgT120I	GGNCC	7 cut(s)	70, 479, 599, 872, 1508, 1553, 2069
BmiI	GGNNCC	8 cut(s)	79, 119, 400, 1093, 1326, 1509, 1642, 1795
BmrFI	CCNGG	8 cut(s)	346, 1009, 1161, 1364, 1557, 1705, 1857, 2155
BmrI	ACTGGG	4 cut(s)	978, 1424, 1832, 1920
BmsI	GCATC	5 cut(s)	637, 793, 820, 895, 968
BmtI	GCTAGC	1 cut(s)	1920
BmuI	ACTGGG	4 cut(s)	978, 1424, 1832, 1920
BpmI	CTGGAG	2 cut(s)	1182, 1726
Bpu1102I	GCTNAGC	1 cut(s)	270
BpuEI	CTTGAG	3 cut(s)	626, 2123, 2177
BpuMI	CCSGG	2 cut(s)	1364, 2155
BsaI	GGTCTC	2 cut(s)	1587, 1594
BsaJI	CCNNGG	9 cut(s)	73, 235, 345, 868, 1007, 1159, 1503, 1556, 2154
BsaWI	WCCGGW	2 cut(s)	191, 1585
BsaXI	ACNNNNNCTCC	6 cut(s)	495, 525, 659, 689, 2227, 2257
Bsc4I	CCNNNNNNNGG	2 cut(s)	1506, 2006
Bse118I	RCCGGY	2 cut(s)	182, 191
Bse1I	ACTGG	5 cut(s)	520, 984, 1430, 1838, 1915
Bse3DI	GCAATG	1 cut(s)	1003
BseBI	CCWGG	6 cut(s)	346, 1009, 1161, 1557, 1705, 1857
BseDI	CCNNGG	9 cut(s)	73, 235, 345, 868, 1007, 1159, 1503, 1556, 2154
BseGI	GGATG	2 cut(s)	832, 1359
BseLI	CCNNNNNNNGG	2 cut(s)	1506, 2006
BseMI	GCAATG	1 cut(s)	1003
BseMII	CTCAG	4 cut(s)	258, 284, 320, 1473
BseNI	ACTGG	5 cut(s)	520, 984, 1430, 1838, 1915
BseRI	GAGGAG	2 cut(s)	516, 1489
BseSI	GKGCMC	2 cut(s)	120, 1244
BseYI	CCCAGC	1 cut(s)	2101
BsgI	GTGCAG	2 cut(s)	768, 1461
BshFI	GGCC	9 cut(s)	71, 445, 536, 601, 725, 873, 1367, 1510, 2070
BshNI	GGYRCC	4 cut(s)	77, 117, 1640, 1793
BshTI	ACCGGT	1 cut(s)	191
BsiHKAI	GWGCWC	3 cut(s)	335, 1244, 2227
BsiHKCI	CYCGRG	1 cut(s)	642
BsiSI	CCGG	5 cut(s)	183, 192, 1364, 1586, 2154
BslFI	GGGAC	1 cut(s)	844
BslI	CCNNNNNNNGG	2 cut(s)	1506, 2006
BsmAI	GTCTC	4 cut(s)	916, 1244, 1587, 1594
BsmFI	GGGAC	1 cut(s)	844
BsmI	GAATGC	2 cut(s)	932, 1183
BsnI	GGCC	9 cut(s)	71, 445, 536, 601, 725, 873, 1367, 1510, 2070
Bso31I	GGTCTC	2 cut(s)	1587, 1594
BsoBI	CYCGRG	1 cut(s)	642
Bsp1286I	GDGCHC	4 cut(s)	120, 335, 1244, 2227
Bsp143I	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
Bsp1720I	GCTNAGC	1 cut(s)	270
Bsp19I	CCATGG	2 cut(s)	73, 1503
BspACI	CCGC	2 cut(s)	726, 744
BspANI	GGCC	9 cut(s)	71, 445, 536, 601, 725, 873, 1367, 1510, 2070
BspCNI	CTCAG	4 cut(s)	257, 283, 321, 1474
BspLI	GGNNCC	8 cut(s)	79, 119, 400, 1093, 1326, 1509, 1642, 1795
BspMAI	CTGCAG	2 cut(s)	1672, 1907
BspMI	ACCTGC	2 cut(s)	1483, 2096
BspOI	GCTAGC	1 cut(s)	1920
BspPI	GGATC	7 cut(s)	697, 872, 1319, 1332, 1778, 1828, 1847
BspT107I	GGYRCC	4 cut(s)	77, 117, 1640, 1793
BspTNI	GGTCTC	2 cut(s)	1587, 1594
BsrDI	GCAATG	1 cut(s)	1003
BsrFI	RCCGGY	2 cut(s)	182, 191
BsrI	ACTGG	5 cut(s)	520, 984, 1430, 1838, 1915
BssAI	RCCGGY	2 cut(s)	182, 191
BssECI	CCNNGG	9 cut(s)	73, 235, 345, 868, 1007, 1159, 1503, 1556, 2154
BssMI	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
BssT1I	CCWWGG	4 cut(s)	73, 235, 868, 1503
Bst2UI	CCWGG	6 cut(s)	346, 1009, 1161, 1557, 1705, 1857
Bst4CI	ACNGT	7 cut(s)	434, 697, 1021, 1130, 2050, 2077, 2122
Bst6I	CTCTTC	1 cut(s)	627
BstAPI	GCANNNNNTGC	4 cut(s)	734, 755, 1148, 1178
BstC8I	GCNNGC	6 cut(s)	489, 994, 1030, 1183, 1213, 1918
BstDEI	CTNAG	6 cut(s)	56, 244, 270, 329, 1482, 1570
BstDSI	CCRYGG	2 cut(s)	73, 1503
BstF5I	GGATG	2 cut(s)	832, 1359
BstH2I	RGCGCY	1 cut(s)	1771
BstHHI	GCGC	1 cut(s)	1770
BstKTI	GATC	8 cut(s)	265, 638, 692, 880, 1327, 1786, 1823, 1855
BstMAI	GTCTC	4 cut(s)	916, 1244, 1587, 1594
BstMBI	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
BstMWI	GCNNNNNNNGC	9 cut(s)	77, 124, 647, 734, 755, 764, 929, 1148, 1178
BstNI	CCWGG	6 cut(s)	346, 1009, 1161, 1557, 1705, 1857
BstNSI	RCATGY	3 cut(s)	783, 996, 1664
BstSCI	CCNGG	8 cut(s)	344, 1007, 1159, 1362, 1555, 1703, 1855, 2153
BstSFI	CTRYAG	3 cut(s)	693, 1668, 1903
BstSLI	GKGCMC	2 cut(s)	120, 1244
BstX2I	RGATCY	4 cut(s)	689, 1324, 1783, 1820
BstYI	RGATCY	4 cut(s)	689, 1324, 1783, 1820
BsuRI	GGCC	9 cut(s)	71, 445, 536, 601, 725, 873, 1367, 1510, 2070
BtgI	CCRYGG	2 cut(s)	73, 1503
BtgZI	GCGATG	1 cut(s)	855
BtsCI	GGATG	2 cut(s)	832, 1359
BtsI	GCAGTG	3 cut(s)	81, 669, 1497
BtsIMutI	CAGTG	7 cut(s)	81, 439, 669, 1017, 1106, 1497, 1908
BveI	ACCTGC	2 cut(s)	1483, 2096
Cac8I	GCNNGC	6 cut(s)	489, 994, 1030, 1183, 1213, 1918
CaiI	CAGNNNCTG	2 cut(s)	734, 1556
CfoI	GCGC	1 cut(s)	1770
Cfr10I	RCCGGY	2 cut(s)	182, 191
Cfr13I	GGNCC	7 cut(s)	70, 479, 599, 872, 1508, 1553, 2069
CseI	GACGC	1 cut(s)	374
Csp6I	GTAC	4 cut(s)	189, 987, 1426, 2046
CspAI	ACCGGT	1 cut(s)	191
CspCI	CAANNNNNGTGG	4 cut(s)	1357, 1392, 1792, 1827
CviQI	GTAC	4 cut(s)	189, 987, 1426, 2046
DdeI	CTNAG	6 cut(s)	56, 244, 270, 329, 1482, 1570
DpnI	GATC	8 cut(s)	264, 637, 691, 879, 1326, 1785, 1822, 1854
DpnII	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
DraI	TTTAAA	1 cut(s)	1101
DrdI	GACNNNNNNGTC	1 cut(s)	663
DseDI	GACNNNNNNGTC	1 cut(s)	663
EaeI	YGGCCR	4 cut(s)	443, 534, 723, 1365
Eam1104I	CTCTTC	1 cut(s)	627
EarI	CTCTTC	1 cut(s)	627
Ecl136II	GAGCTC	1 cut(s)	2225
Eco130I	CCWWGG	4 cut(s)	73, 235, 868, 1503
Eco24I	GRGCYC	1 cut(s)	2227
Eco31I	GGTCTC	2 cut(s)	1587, 1594
Eco47I	GGWCC	2 cut(s)	479, 1553
Eco53kI	GAGCTC	1 cut(s)	2225
Eco57I	CTGAAG	3 cut(s)	159, 616, 1130
Eco88I	CYCGRG	1 cut(s)	642
EcoICRI	GAGCTC	1 cut(s)	2225
EcoO109I	RGGNCCY	2 cut(s)	479, 1553
EcoRI	GAATTC	1 cut(s)	1046
EcoRII	CCWGG	6 cut(s)	344, 1007, 1159, 1555, 1703, 1855
EcoT14I	CCWWGG	4 cut(s)	73, 235, 868, 1503
EcoT22I	ATGCAT	1 cut(s)	934
EcoT38I	GRGCYC	1 cut(s)	2227
ErhI	CCWWGG	4 cut(s)	73, 235, 868, 1503
FalI	AAGNNNNNCTT	4 cut(s)	195, 227, 933, 965
FaqI	GGGAC	1 cut(s)	844
FauNDI	CATATG	1 cut(s)	2089
FblI	GTMKAC	2 cut(s)	363, 2094
FokI	GGATG	2 cut(s)	839, 1346
FriOI	GRGCYC	1 cut(s)	2227
FspBI	CTAG	5 cut(s)	356, 1437, 1754, 1917, 1952
GlaI	GCGC	1 cut(s)	1769
GsaI	CCCAGC	1 cut(s)	2105
GsuI	CTGGAG	2 cut(s)	1182, 1726
HaeII	RGCGCY	1 cut(s)	1771
HaeIII	GGCC	9 cut(s)	71, 445, 536, 601, 725, 873, 1367, 1510, 2070
HapII	CCGG	5 cut(s)	183, 192, 1364, 1586, 2154
HgaI	GACGC	1 cut(s)	374
HhaI	GCGC	1 cut(s)	1770
Hin6I	GCGC	1 cut(s)	1768
HinP1I	GCGC	1 cut(s)	1768
HindIII	AAGCTT	4 cut(s)	251, 1183, 1213, 2209
HpaII	CCGG	5 cut(s)	183, 192, 1364, 1586, 2154
HphI	GGTGA	4 cut(s)	31, 172, 1573, 2176
Hpy166II	GTNNAC	7 cut(s)	109, 197, 364, 1242, 1658, 2095, 2145
Hpy188III	TCNNGA	8 cut(s)	19, 644, 824, 937, 1382, 1754, 1824, 2194
Hpy8I	GTNNAC	7 cut(s)	109, 197, 364, 1242, 1658, 2095, 2145
HpyAV	CCTTC	8 cut(s)	53, 464, 821, 1710, 1980, 1989, 2010, 2022
HpyCH4III	ACNGT	7 cut(s)	434, 697, 1021, 1130, 2050, 2077, 2122
HpyCH4IV	ACGT	1 cut(s)	1059
HpyF10VI	GCNNNNNNNGC	9 cut(s)	77, 124, 647, 734, 755, 764, 929, 1148, 1178
HpyF3I	CTNAG	6 cut(s)	56, 244, 270, 329, 1482, 1570
HpySE526I	ACGT	1 cut(s)	1059
HspAI	GCGC	1 cut(s)	1768
Kzo9I	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
LmnI	GCTCC	3 cut(s)	920, 1009, 2222
LweI	GCATC	5 cut(s)	637, 793, 820, 895, 968
MaeI	CTAG	5 cut(s)	356, 1437, 1754, 1917, 1952
MaeII	ACGT	1 cut(s)	1059
MaeIII	GTNAC	9 cut(s)	178, 511, 665, 697, 1165, 1678, 2050, 2116, 2122
MalI	GATC	8 cut(s)	264, 637, 691, 879, 1326, 1785, 1822, 1854
MboI	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
MboII	GAAGA	5 cut(s)	614, 622, 914, 1123, 1397
MfeI	CAATTG	1 cut(s)	1848
MflI	RGATCY	4 cut(s)	689, 1324, 1783, 1820
MhlI	GDGCHC	4 cut(s)	120, 335, 1244, 2227
MlsI	TGGCCA	2 cut(s)	445, 536
MluNI	TGGCCA	2 cut(s)	445, 536
MlyI	GAGTC	6 cut(s)	458, 617, 673, 1173, 1717, 2269
Mox20I	TGGCCA	2 cut(s)	445, 536
Mph1103I	ATGCAT	1 cut(s)	934
MscI	TGGCCA	2 cut(s)	445, 536
MseI	TTAA	7 cut(s)	213, 1100, 1217, 1221, 1833, 1992, 2274
MslI	CAYNNNNRTG	2 cut(s)	1646, 2009
Msp20I	TGGCCA	2 cut(s)	445, 536
MspA1I	CMGCKG	1 cut(s)	1154
MspI	CCGG	5 cut(s)	183, 192, 1364, 1586, 2154
MspR9I	CCNGG	8 cut(s)	346, 1009, 1161, 1364, 1557, 1705, 1857, 2155
MunI	CAATTG	1 cut(s)	1848
Mva1269I	GAATGC	2 cut(s)	932, 1183
MvaI	CCWGG	6 cut(s)	346, 1009, 1161, 1557, 1705, 1857
MwoI	GCNNNNNNNGC	9 cut(s)	77, 124, 647, 734, 755, 764, 929, 1148, 1178
NciI	CCSGG	2 cut(s)	1364, 2155
NcoI	CCATGG	2 cut(s)	73, 1503
NdeI	CATATG	1 cut(s)	2089
NdeII	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
NheI	GCTAGC	1 cut(s)	1916
NlaIV	GGNNCC	8 cut(s)	79, 119, 400, 1093, 1326, 1509, 1642, 1795
NmuCI	GTSAC	3 cut(s)	178, 665, 1165
NsiI	ATGCAT	1 cut(s)	934
NspI	RCATGY	3 cut(s)	783, 996, 1664
PacI	TTAATTAA	1 cut(s)	1221
PaeI	GCATGC	1 cut(s)	996
PaeR7I	CTCGAG	1 cut(s)	642
PaqCI	CACCTGC	1 cut(s)	2096
PciI	ACATGT	1 cut(s)	1660
PctI	GAATGC	2 cut(s)	932, 1183
PfeI	GAWTC	6 cut(s)	140, 166, 290, 1012, 1315, 1877
PfoI	TCCNGGA	2 cut(s)	1703, 1855
PinAI	ACCGGT	1 cut(s)	191
PleI	GAGTC	6 cut(s)	457, 616, 672, 1172, 1716, 2268
PpsI	GAGTC	6 cut(s)	457, 616, 672, 1172, 1716, 2268
PpuMI	RGGWCCY	2 cut(s)	479, 1553
PscI	ACATGT	1 cut(s)	1660
PsiI	TTATAA	1 cut(s)	1626
Psp124BI	GAGCTC	1 cut(s)	2227
Psp1406I	AACGTT	1 cut(s)	1059
Psp5II	RGGWCCY	2 cut(s)	479, 1553
Psp6I	CCWGG	6 cut(s)	344, 1007, 1159, 1555, 1703, 1855
PspFI	CCCAGC	1 cut(s)	2101
PspGI	CCWGG	6 cut(s)	344, 1007, 1159, 1555, 1703, 1855
PspN4I	GGNNCC	8 cut(s)	79, 119, 400, 1093, 1326, 1509, 1642, 1795
PspPI	GGNCC	7 cut(s)	70, 479, 599, 872, 1508, 1553, 2069
PspPPI	RGGWCCY	2 cut(s)	479, 1553
PstI	CTGCAG	2 cut(s)	1672, 1907
PstNI	CAGNNNCTG	2 cut(s)	734, 1556
PsuI	RGATCY	4 cut(s)	689, 1324, 1783, 1820
PvuII	CAGCTG	1 cut(s)	1154
RsaI	GTAC	4 cut(s)	190, 988, 1427, 2047
RsaNI	GTAC	4 cut(s)	189, 987, 1426, 2046
RseI	CAYNNNNRTG	2 cut(s)	1646, 2009
SacI	GAGCTC	1 cut(s)	2227
SaqAI	TTAA	7 cut(s)	213, 1100, 1217, 1221, 1833, 1992, 2274
Sau3AI	GATC	8 cut(s)	262, 635, 689, 877, 1324, 1783, 1820, 1852
Sau96I	GGNCC	7 cut(s)	70, 479, 599, 872, 1508, 1553, 2069
ScaI	AGTACT	1 cut(s)	988
SchI	GAGTC	6 cut(s)	458, 617, 673, 1173, 1717, 2269
ScrFI	CCNGG	8 cut(s)	346, 1009, 1161, 1364, 1557, 1705, 1857, 2155
SduI	GDGCHC	4 cut(s)	120, 335, 1244, 2227
SfaNI	GCATC	5 cut(s)	637, 793, 820, 895, 968
SfcI	CTRYAG	3 cut(s)	693, 1668, 1903
Sfr274I	CTCGAG	1 cut(s)	642
SinI	GGWCC	2 cut(s)	479, 1553
SlaI	CTCGAG	1 cut(s)	642
SmiMI	CAYNNNNRTG	2 cut(s)	1646, 2009
SmlI	CTYRAG	4 cut(s)	605, 642, 2138, 2192
SmoI	CTYRAG	4 cut(s)	605, 642, 2138, 2192
SphI	GCATGC	1 cut(s)	996
SsiI	CCGC	2 cut(s)	726, 744
SspMI	CTAG	5 cut(s)	356, 1437, 1754, 1917, 1952
SstI	GAGCTC	1 cut(s)	2227
StyD4I	CCNGG	8 cut(s)	344, 1007, 1159, 1362, 1555, 1703, 1855, 2153
StyI	CCWWGG	4 cut(s)	73, 235, 868, 1503
TaaI	ACNGT	7 cut(s)	434, 697, 1021, 1130, 2050, 2077, 2122
TaiI	ACGT	1 cut(s)	1062
TaqI	TCGA	4 cut(s)	643, 849, 1646, 1893
TatI	WGTACW	1 cut(s)	986
TauI	GCSGC	1 cut(s)	728
TfiI	GAWTC	6 cut(s)	140, 166, 290, 1012, 1315, 1877
Tru1I	TTAA	7 cut(s)	213, 1100, 1217, 1221, 1833, 1992, 2274
Tru9I	TTAA	7 cut(s)	213, 1100, 1217, 1221, 1833, 1992, 2274
TscAI	CASTG	7 cut(s)	88, 439, 676, 1024, 1113, 1497, 1915
TseFI	GTSAC	3 cut(s)	178, 665, 1165
Tsp45I	GTSAC	3 cut(s)	178, 665, 1165
TspDTI	ATGAA	1 cut(s)	483
TspGWI	ACGGA	1 cut(s)	1833
TspRI	CASTG	7 cut(s)	88, 439, 676, 1024, 1113, 1497, 1915
VneI	GTGCAC	1 cut(s)	1240
VpaK11BI	GGWCC	2 cut(s)	479, 1553
XapI	RAATTY	4 cut(s)	494, 523, 1046, 1072
XbaI	TCTAGA	1 cut(s)	1753
XceI	RCATGY	3 cut(s)	783, 996, 1664
XhoI	CTCGAG	1 cut(s)	642
XmiI	GTMKAC	2 cut(s)	363, 2094
XspI	CTAG	5 cut(s)	356, 1437, 1754, 1917, 1952
ZrmI	AGTACT	1 cut(s)	988
Zsp2I	ATGCAT	1 cut(s)	934

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.