Rmu_sc0014980.1_g000003

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014980.1
OG0002713
Physical Location & Seq
Reverse (-)
18415 .. 19181
767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014980.1_g000003.1.cds

Sequence Viewer

Length: 645 bp
atgtcttgtccacatgtatctgcagttgcagcaactttgaaatcataccgaccttcctggagtccagcagccataatgtctgcaataatgacaacatcaacaactctagacaacaacaaacgccctattgggagagatccaaatggcacccctaccacaccttttgactacggatctggacacattaacccagttgcagcaattgatcctggacttgtatatgattttgattcccatgacataatcgactttctctgcagcactggggctagcactctgcaactgaaaaacctcacgggtaatctagtttattgccagaagccttctataccttcctacaattttaactacccttccattggtgtgtcaaaaatgagtggaaggctatctgttcagcgtacagttacttattacggcaaaggcccaacagtatatgctgcatatgtagactacccagcaggtgtaaatgttacagtaacaccagctaaactcaagtttacaaataccggggaaaagatgtctttcagtgttgatttcacccccctcaagaatagtaatggaagctttgtgtttggagctctgacatggagtaatggtattcagaatgttaggagtcctattggtcttaatgtgctttcaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

214

Amino Acids

22.95

Weight (kDa)

9.1

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 146
AccI GTMKAC 1 cut(s) 447
AclWI GGATC 3 cut(s) 131, 181, 200
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 13
AgsI TTSAA 2 cut(s) 40, 639
AjnI CCWGG 2 cut(s) 56, 208
AluBI AGCT 3 cut(s) 485, 564, 578
AluI AGCT 3 cut(s) 485, 564, 578
Alw21I GWGCWC 1 cut(s) 580
AlwI GGATC 3 cut(s) 131, 181, 200
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 5 cut(s) 29, 68, 197, 258, 437
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 529
AsuNHI GCTAGC 1 cut(s) 269
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 5 cut(s) 41, 80, 209, 270, 424
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 2 cut(s) 58, 210
BcnI CCSGG 1 cut(s) 508
BfaI CTAG 3 cut(s) 107, 270, 305
BfmI CTRYAG 2 cut(s) 21, 256
BfuAI ACCTGC 1 cut(s) 449
BisI GCNGC 5 cut(s) 30, 69, 198, 259, 438
BlsI GCNGC 5 cut(s) 31, 70, 199, 260, 439
Bme1390I CCNGG 3 cut(s) 58, 210, 508
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 3 cut(s) 58, 210, 508
BmrI ACTGGG 2 cut(s) 185, 273
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 2 cut(s) 185, 273
BpmI CTGGAG 1 cut(s) 79
BpuEI CTTGAG 2 cut(s) 476, 530
BpuMI CCSGG 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 580, 610
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 191, 268
BseBI CCWGG 2 cut(s) 58, 210
BseDI CCNNGG 1 cut(s) 507
BseLI CCNNNNNNNGG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 191, 268
BseXI GCAGC 5 cut(s) 41, 80, 209, 270, 424
BseYI CCCAGC 1 cut(s) 454
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 359
BsnI GGCC 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 3 cut(s) 136, 173, 205
BspANI GGCC 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 148
BspMAI CTGCAG 2 cut(s) 25, 260
BspMI ACCTGC 1 cut(s) 449
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 131, 181, 200
BspT107I GGYRCC 1 cut(s) 146
BsrI ACTGG 2 cut(s) 191, 268
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 3 cut(s) 136, 173, 205
Bst2UI CCWGG 2 cut(s) 58, 210
Bst4CI ACNGT 3 cut(s) 403, 430, 475
BstC8I GCNNGC 1 cut(s) 271
BstKTI GATC 3 cut(s) 139, 176, 208
BstMBI GATC 3 cut(s) 136, 173, 205
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 2 cut(s) 58, 210
BstNSI RCATGY 1 cut(s) 17
BstSCI CCNGG 3 cut(s) 56, 208, 506
BstSFI CTRYAG 2 cut(s) 21, 256
BstV1I GCAGC 5 cut(s) 41, 80, 209, 270, 424
BstX2I RGATCY 2 cut(s) 136, 173
BstYI RGATCY 2 cut(s) 136, 173
BsuRI GGCC 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 261, 532
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 399
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviAII CATG 3 cut(s) 14, 236, 585
CviJI RGCY 8 cut(s) 71, 269, 322, 385, 423, 485, 564, 578
CviKI_1 RGCY 8 cut(s) 71, 269, 322, 385, 423, 485, 564, 578
CviQI GTAC 1 cut(s) 399
DpnI GATC 3 cut(s) 138, 175, 207
DpnII GATC 3 cut(s) 136, 173, 205
Ecl136II GAGCTC 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 56, 208
EcoT38I GRGCYC 1 cut(s) 580
FaeI CATG 3 cut(s) 17, 239, 588
FatI CATG 3 cut(s) 13, 235, 584
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 5 cut(s) 30, 69, 198, 259, 438
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 5 cut(s) 30, 69, 198, 259, 438
FspBI CTAG 3 cut(s) 107, 270, 305
GluI GCNGC 5 cut(s) 30, 69, 198, 259, 438
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 1 cut(s) 423
HapII CCGG 1 cut(s) 507
Hin1II CATG 3 cut(s) 17, 239, 588
HindIII AAGCTT 1 cut(s) 562
HinfI GANTC 3 cut(s) 61, 230, 613
HpaII CCGG 1 cut(s) 507
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 11, 448, 498
Hpy188I TCNGA 2 cut(s) 582, 603
Hpy188III TCNNGA 3 cut(s) 107, 177, 547
Hpy8I GTNNAC 3 cut(s) 11, 448, 498
HpyAV CCTTC 5 cut(s) 63, 333, 342, 363, 375
HpyCH4III ACNGT 3 cut(s) 403, 430, 475
HpyCH4V TGCA 7 cut(s) 23, 29, 83, 197, 258, 280, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
Hsp92II CATG 3 cut(s) 17, 239, 588
Kzo9I GATC 3 cut(s) 136, 173, 205
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 5 cut(s) 41, 80, 209, 270, 424
MaeI CTAG 3 cut(s) 107, 270, 305
MaeIII GTNAC 3 cut(s) 403, 469, 475
MalI GATC 3 cut(s) 138, 175, 207
MboI GATC 3 cut(s) 136, 173, 205
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 136, 173
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 2 cut(s) 201, 340
MlyI GAGTC 2 cut(s) 70, 622
MnlI CCTC 2 cut(s) 302, 554
MseI TTAA 3 cut(s) 186, 345, 627
MslI CAYNNNNRTG 1 cut(s) 362
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 3 cut(s) 58, 210, 508
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 2 cut(s) 58, 210
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 1 cut(s) 508
NdeI CATATG 1 cut(s) 442
NdeII GATC 3 cut(s) 136, 173, 205
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 3 cut(s) 17, 239, 588
NlaIV GGNNCC 1 cut(s) 148
NspI RCATGY 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 449
PciI ACATGT 1 cut(s) 13
PfeI GAWTC 1 cut(s) 230
PfoI TCCNGGA 2 cut(s) 56, 208
PkrI GCNGC 5 cut(s) 31, 70, 199, 260, 439
PleI GAGTC 2 cut(s) 69, 621
PpsI GAGTC 2 cut(s) 69, 621
PscI ACATGT 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 56, 208
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 2 cut(s) 56, 208
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 422
PstI CTGCAG 2 cut(s) 25, 260
PsuI RGATCY 2 cut(s) 136, 173
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 580
SaqAI TTAA 3 cut(s) 186, 345, 627
SatI GCNGC 5 cut(s) 30, 69, 198, 259, 438
Sau3AI GATC 3 cut(s) 136, 173, 205
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 2 cut(s) 70, 622
ScrFI CCNGG 3 cut(s) 58, 210, 508
SduI GDGCHC 1 cut(s) 580
SetI ASST 8 cut(s) 55, 163, 294, 334, 463, 487, 566, 580
SfcI CTRYAG 2 cut(s) 21, 256
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 491, 545
SmoI CTYRAG 2 cut(s) 491, 545
Sse9I AATT 2 cut(s) 201, 340
SspMI CTAG 3 cut(s) 107, 270, 305
SstI GAGCTC 1 cut(s) 580
StyD4I CCNGG 3 cut(s) 56, 208, 506
TaaI ACNGT 3 cut(s) 403, 430, 475
TaqI TCGA 1 cut(s) 246
TasI AATT 2 cut(s) 201, 340
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 3 cut(s) 186, 345, 627
Tru9I TTAA 3 cut(s) 186, 345, 627
TscAI CASTG 2 cut(s) 268, 532
TseI GCWGC 5 cut(s) 29, 68, 197, 258, 437
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 2 cut(s) 268, 532
XbaI TCTAGA 1 cut(s) 106
XceI RCATGY 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 3 cut(s) 107, 270, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.