MD17G1012100.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002713
Physical Location & Seq
Reverse (-)
1092371 .. 1094489
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1012100.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGATGTGAGGCAAAGAAGAGGGTTAGCGCCAGCGGGATCACCAAATTATTGTGTCTCCCCTCAAAGCGATAACAATGCTGCTAATAGGGGTTATGGTATGAGATCATATGGCTATTTACGTCGCTGTGCCCGTGGTAATTTTGTTCCCCCTATTAAATCTAATGAGGGAAATGTTGGGAATGTGACTTCACGAGTTGTTGGAAAGTCAGATGATGCATTAGGTGACTCAACTAAGAGATGTTTGGAATTGCTCTGTGGTCCTGATGGAGAGCTCCCAGAAAAATTAAGGAACTTGGAACCTCGTCTTCTTGAACATGTTAGTAATGAGATTATGGACAAGGATCCGACTGTTCGATGGAATGATATTGCTGGTTTGGAGCATGCTAAGAAATGTGTAACCGAGATGGTCATATGGCCTCTACTACGTCCTGACATTTTTAAAGGTTGCCGTTCTCCTGGGAAAGGCCTTCTTCTCTTTGGTCCACCAGGAACTGGAAAAACAATGATCGGGAAAGCCATAGCTGGAGAAGCAAAAGCAACCTTTTTCTACATATCTGCTAGTTCATTAACAAGCAAATGGATTGGTGAGGGCGAAAAGCTAGTGCGAGCACTGTTTGGAGTTGCAAGTTGTCGTCAGCCTGCTGTAATTTTTGTTGATGAAATTGATTCACTTCTCTCACAGCGTAAGTCAGAAGGTGAGCATGAATCAAGTAGGCGACTCAAAACACAGTTCCTTATTGAGATGGAAGGCTTTGACAGTGGCAGTGAGCAAATTCTTCTTATAGGAGCAACGAATCGACCCCAAGAACTTGATGAAGCAGCAAGGAGGCGACTTACCAAGAGGCTTTACATTCCCCTGCCTTCATCAGCGAGAGCTTGGATCATACGGAATCTCTTAGAAAAAGATGGGACTGTTCAAACTTTCAAGGGAGGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000228 GO:0000280 GO:0000287 GO:0001503 GO:0001649 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006163 GO:0006282 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007276 GO:0008150 GO:0008152 GO:0008283 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009314 GO:0009628 GO:0009892 GO:0009987 GO:0010212 GO:0010520 GO:0010564 GO:0010569 GO:0010605 GO:0010639 GO:0010941 GO:0010948 GO:0016043 GO:0016787 GO:0017144 GO:0019219 GO:0019222 GO:0019637 GO:0019693 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033687 GO:0034641 GO:0040020 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046034 GO:0046483 GO:0046872 GO:0048232 GO:0048285 GO:0048471 GO:0048519 GO:0048523 GO:0048583 GO:0048609 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051321 GO:0051445 GO:0051447 GO:0051704 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0055086 GO:0060255 GO:0060548 GO:0060631 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0071840 GO:0072521 GO:0080090 GO:0080134 GO:0080135 GO:0104004 GO:0110029 GO:0140013 GO:1901135 GO:1901360 GO:1901564 GO:1903046 GO:2000241 GO:2000242 GO:2000779 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Vps4_C

Protein Analysis

314

Amino Acids

34.54

Weight (kDa)

9.22

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 157 - 287 5.8e-40 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 494
AciI CCGC 1 cut(s) 35
AclWI GGATC 4 cut(s) 46, 338, 351, 890
AcsI RAATTY 1 cut(s) 774
AfiI CCNNNNNNNGG 2 cut(s) 464, 494
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 3 cut(s) 314, 920, 928
AjnI CCWGG 2 cut(s) 457, 487
AluBI AGCT 4 cut(s) 274, 524, 601, 878
AluI AGCT 4 cut(s) 274, 524, 601, 878
Alw21I GWGCWC 2 cut(s) 276, 613
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 4 cut(s) 46, 338, 351, 890
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 2 cut(s) 416, 466
ApeKI GCWGC 2 cut(s) 80, 821
ApoI RAATTY 1 cut(s) 774
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 260, 482
AsuHPI GGTGA 4 cut(s) 33, 236, 599, 710
AvaII GGWCC 2 cut(s) 260, 482
BaeGI GKGCMC 1 cut(s) 133
BamHI GGATCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 276, 613
BbvI GCAGC 2 cut(s) 67, 833
BccI CCATC 5 cut(s) 260, 351, 400, 739, 902
BceAI ACGGC 1 cut(s) 435
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 2 cut(s) 459, 489
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 561, 602
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 2 cut(s) 81, 822
BlsI GCNGC 2 cut(s) 82, 823
Bme1390I CCNGG 2 cut(s) 459, 489
Bme18I GGWCC 2 cut(s) 260, 482
BmgT120I GGNCC 2 cut(s) 260, 482
BmiI GGNNCC 2 cut(s) 300, 345
BmrFI CCNGG 2 cut(s) 459, 489
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 133, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 494
Bse1I ACTGG 1 cut(s) 499
BseBI CCWGG 2 cut(s) 459, 489
BseDI CCNNGG 2 cut(s) 133, 458
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 464, 494
BseNI ACTGG 1 cut(s) 499
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 2 cut(s) 67, 833
BshFI GGCC 2 cut(s) 418, 468
BsiHKAI GWGCWC 2 cut(s) 276, 613
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 2 cut(s) 464, 494
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 2 cut(s) 418, 468
Bsp1286I GDGCHC 3 cut(s) 133, 276, 613
Bsp143I GATC 5 cut(s) 38, 104, 343, 507, 882
BspACI CCGC 1 cut(s) 35
BspANI GGCC 2 cut(s) 418, 468
BspLI GGNNCC 2 cut(s) 300, 345
BspPI GGATC 4 cut(s) 46, 338, 351, 890
BsrI ACTGG 1 cut(s) 499
BssECI CCNNGG 2 cut(s) 133, 458
BssMI GATC 5 cut(s) 38, 104, 343, 507, 882
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 2 cut(s) 459, 489
Bst4CI ACNGT 5 cut(s) 352, 615, 732, 761, 916
Bst6I CTCTTC 1 cut(s) 13
BstC8I GCNNGC 4 cut(s) 33, 384, 609, 642
BstDEI CTNAG 3 cut(s) 234, 387, 898
BstDSI CCRYGG 1 cut(s) 133
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 5 cut(s) 41, 107, 346, 510, 885
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 38, 104, 343, 507, 882
BstMWI GCNNNNNNNGC 1 cut(s) 530
BstNI CCWGG 2 cut(s) 459, 489
BstNSI RCATGY 2 cut(s) 320, 386
BstSCI CCNGG 2 cut(s) 457, 487
BstSLI GKGCMC 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 67, 833
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 2 cut(s) 418, 468
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 772
BtsIMutI CAGTG 3 cut(s) 611, 766, 772
Cac8I GCNNGC 4 cut(s) 33, 384, 609, 642
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 260, 482
CviAII CATG 3 cut(s) 317, 383, 704
DdeI CTNAG 3 cut(s) 234, 387, 898
DpnI GATC 5 cut(s) 40, 106, 345, 509, 884
DpnII GATC 5 cut(s) 38, 104, 343, 507, 882
DraI TTTAAA 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 274
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 260, 482
Eco53kI GAGCTC 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 457, 487
EcoT22I ATGCAT 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 3 cut(s) 320, 386, 707
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FaqI GGGAC 1 cut(s) 925
FatI CATG 3 cut(s) 316, 382, 703
FauI CCCGC 1 cut(s) 28
FauNDI CATATG 2 cut(s) 109, 413
Fnu4HI GCNGC 2 cut(s) 81, 822
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 81, 822
FspBI CTAG 2 cut(s) 561, 602
GlaI GCGC 1 cut(s) 30
GluI GCNGC 2 cut(s) 81, 822
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 418, 468
HhaI GCGC 1 cut(s) 31
Hin1II CATG 3 cut(s) 320, 386, 707
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HinfI GANTC 6 cut(s) 227, 668, 707, 720, 796, 892
HphI GGTGA 4 cut(s) 33, 236, 599, 710
Hpy166II GTNNAC 1 cut(s) 485
Hpy188I TCNGA 3 cut(s) 211, 348, 694
Hpy188III TCNNGA 5 cut(s) 192, 263, 311, 431, 511
Hpy8I GTNNAC 1 cut(s) 485
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 4 cut(s) 479, 689, 743, 873
HpyCH4III ACNGT 5 cut(s) 352, 615, 732, 761, 916
HpyCH4IV ACGT 2 cut(s) 121, 427
HpyCH4V TGCA 2 cut(s) 218, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 530
HpyF3I CTNAG 3 cut(s) 234, 387, 898
HpySE526I ACGT 2 cut(s) 121, 427
Hsp92II CATG 3 cut(s) 320, 386, 707
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 5 cut(s) 38, 104, 343, 507, 882
LmnI GCTCC 3 cut(s) 279, 379, 788
Lsp1109I GCAGC 2 cut(s) 67, 833
LweI GCATC 1 cut(s) 205
MaeI CTAG 2 cut(s) 561, 602
MaeII ACGT 2 cut(s) 121, 427
MaeIII GTNAC 3 cut(s) 184, 224, 397
MalI GATC 5 cut(s) 40, 106, 345, 509, 884
MboI GATC 5 cut(s) 38, 104, 343, 507, 882
MboII GAAGA 4 cut(s) 30, 299, 464, 770
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 3 cut(s) 133, 276, 613
MluCI AATT 7 cut(s) 46, 139, 248, 284, 648, 663, 774
MlyI GAGTC 2 cut(s) 221, 714
MmeI TCCRAC 2 cut(s) 181, 371
Mph1103I ATGCAT 1 cut(s) 220
MseI TTAA 4 cut(s) 156, 287, 441, 569
MspA1I CMGCKG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 459, 489
MvaI CCWGG 2 cut(s) 459, 489
MwoI GCNNNNNNNGC 1 cut(s) 530
NdeI CATATG 2 cut(s) 109, 413
NdeII GATC 5 cut(s) 38, 104, 343, 507, 882
NlaIII CATG 3 cut(s) 320, 386, 707
NlaIV GGNNCC 2 cut(s) 300, 345
NmuCI GTSAC 2 cut(s) 184, 224
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 2 cut(s) 320, 386
PaeI GCATGC 1 cut(s) 386
PceI AGGCCT 1 cut(s) 468
PciI ACATGT 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PfeI GAWTC 4 cut(s) 668, 707, 796, 892
PflMI CCANNNNNTGG 1 cut(s) 494
PkrI GCNGC 2 cut(s) 82, 823
PleI GAGTC 2 cut(s) 221, 714
PpsI GAGTC 2 cut(s) 221, 714
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 457, 487
PspGI CCWGG 2 cut(s) 457, 487
PspN4I GGNNCC 2 cut(s) 300, 345
PspPI GGNCC 2 cut(s) 260, 482
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 343
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 4 cut(s) 156, 287, 441, 569
SatI GCNGC 2 cut(s) 81, 822
Sau3AI GATC 5 cut(s) 38, 104, 343, 507, 882
Sau96I GGNCC 2 cut(s) 260, 482
SchI GAGTC 2 cut(s) 221, 714
ScrFI CCNGG 2 cut(s) 459, 489
SduI GDGCHC 3 cut(s) 133, 276, 613
SfaNI GCATC 1 cut(s) 205
SinI GGWCC 2 cut(s) 260, 482
SphI GCATGC 1 cut(s) 386
Sse9I AATT 7 cut(s) 46, 139, 248, 284, 648, 663, 774
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 1 cut(s) 35
SspMI CTAG 2 cut(s) 561, 602
SstI GAGCTC 1 cut(s) 276
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 457, 487
TaaI ACNGT 5 cut(s) 352, 615, 732, 761, 916
TaiI ACGT 2 cut(s) 124, 430
TaqI TCGA 2 cut(s) 355, 799
TasI AATT 7 cut(s) 46, 139, 248, 284, 648, 663, 774
TfiI GAWTC 4 cut(s) 668, 707, 796, 892
Tru1I TTAA 4 cut(s) 156, 287, 441, 569
Tru9I TTAA 4 cut(s) 156, 287, 441, 569
TscAI CASTG 3 cut(s) 618, 766, 772
TseFI GTSAC 2 cut(s) 184, 224
TseI GCWGC 2 cut(s) 80, 821
Tsp45I GTSAC 2 cut(s) 184, 224
TspDTI ATGAA 5 cut(s) 555, 675, 720, 831, 855
TspGWI ACGGA 1 cut(s) 904
TspRI CASTG 3 cut(s) 618, 766, 772
Van91I CCANNNNNTGG 1 cut(s) 494
VpaK11BI GGWCC 2 cut(s) 260, 482
XagI CCTNNNNNAGG 1 cut(s) 462
XapI RAATTY 1 cut(s) 774
XceI RCATGY 2 cut(s) 320, 386
XspI CTAG 2 cut(s) 561, 602
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.