Rh5BG303600

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002713
Physical Location & Seq
Reverse (-)
39228324 .. 39239139
10816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG303600.1

Sequence Viewer

Length: 2592 bp
ATGTTCTTTTTCAAGAATTCTGATAATATGTTCATACCTTGTGGACCAAAGCAACCGGGTGCGGTCCAAATCACCATGCAGGAGTTGGCAGCTCAAGGACTAGCTTCACAGATCCTTCCACCTCCCATCACAAAAACAGATTTTGATAAGGTACTTGCTAGACAGAGGCCTACTGTGAGCAAAGGGGACCTTGAAGTCCATGAAAGTTTCTTCAATGTGGAAGGATTGGATGTGCCTCTGATTCTCCTGTATAGATCTAGGCTGTTGAGTCATACAGATCTGTTTTTTTCCCCTCCGCTCCCCCAAAATGGAAAGAAACTTGGGTGTTGGGCAAAATATGCCCGTTCCTCGCATAATGGACTTTCTTTGGCCCATGGAGCCACTGCTGACACAAAGCACTATATTGTTTACATGGGGCACCACTCGCACACCAACTCGGAATCTGTCATCAGAGCTAACCATGAGATTCTTGCTTCAGTCACCGGCAGTACCGGTGGACAAGAAGCAGCACTTAACCATTACAGTAAAAGTTTCCAAGGCTTCTCAGCCAAGCTTACACCAGATCAAGCTCAGCAACTTGCTGAACATGATTTGGTTATTTCGGTGTTTGAGAGCAAGACAAACAAACTGAGCACAACACATTCCTGGGATTTTCTAGGCGTAGACGCTACTCCTAATTACAATCAAATGCCAATGGGTTCCAAATCCAATGTCATTGTTGGTGTAATTGACAGTGGAGTTTGGCCAGAGTCAGAGAGCTTCAATGATGAAGGATTAGGACCTGTGCCAGCTAAATTCAGAGGAGAGTGTGTTACTGGTCAGAATTTTACATTGGCCAACTGCAACAGAAAAATCATAGGCTCTCGCTTCTATTATCATGGATTTGAAGTAGAAAATGGGCCACTTGAGTCCTTTGCACCTCTTCCTTTCTTCAGATCAGCTCGAGATGCTGATGGACATGGGAGTCACACTGCCTCCACGATAGCTGGATCCACAGTAACTAATGTCAGCTTCTTGGGAATGGCCACAGGAACTGCAAGAGGCGGTGCAGAAAGTGCCAGACTTGCTATCTACAAGGCATGTTGGTTCGGCTTGTGTAGTGATGCAGACCTTCTTTCAGCTCTGGATGATGCCATTGGTGATGGTGTCGATATTCTGTCCCTTTCCCTTGGTCCTGATCCTCCACAGCCAACATATTTTGAAGATGCAGTCTCCATTGGAGCTTTCCATGCATTCCAGAAAGGAATACTTGTTTCTGCATCAGCAGGGAACTCTGGTTTCCCCAGTACTGCATGCAATGTAGCTCCCTGGATTCTCACTATTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTTTATCTGGGAAATTCACAAATACTAAAGGGTTCCTCTTTAAACCCACTAAAGATGGATAAGTCTTACAGTTTGATTGCTGCAAGTGTTGCAGCTGCCCCTGGAGTCACAGCAAAGAATGCAAGCTTCTGCAAGGACAAAACTCTAAATGCAAGCTTAATTAAGGGAAAAATCGTTGTGTGCACCTTTGAGACATTTACCGACAACCGAAGGGAAAAAAGTATAGTTGTAAGACAAGGTGGTGGTGTAGGAATGATTCTTGTGGATCCATTTCTCAAAGATGTTGGCTTTGAGTTTGTCATCCCGGCCACCCTAATTGGTCAAGAAGAAGCAGCACAGCTTCAAGCATACATGACAACAGAGAAGTACCCAGTTGCTAGAATCTCACAAACGACAACAATTCTGAAAACTAAACCTGCACCTGAGATGGCAGTGTTCTCCTCCATGGGGCCAAATATCATAACCCCAGACATTATCAAACCAGATATTACAGGACCTGGGGTGAATATCTTAGCAGCATGGTCTCCGGTCTCAACTACACCCACAGCAGAAAGGTCTGTCAATTATAACATCATTTCTGGCACCTCGATGTCTTGTCCACATGTATCTGCAGTTGCAGCAACTTTGAAATCATACCGACCTTCCTGGAGTCCAGCAGCCATAATGTCTGCAATAATGACAACATCAACAACTCTAGACAACAACAAACGCCCTATTGGGAGAGATCCAAATGGCACCCCTACCACACCTTTTGACTACGGATCTGGACACATTAACCCAGTTGCAGCAATTGATCCTGGACTTGTATATGATTTTGATTCCCATGACATAATCGACTTTCTCTGCAGCACTGGGGCTAGCACTCTGCAACTGAAAAACCTCACGGGTAATCTAGTTTATTGCCAGAAGCCTTCTATACCTTCCTACAATTTTAACTACCCTTCCATTGGCGTGTCAAAAATGAGTGGAAGGCTATCTGTTCAGCGTACAGTTACTTATTACGGCAAAGGCCCAACGGTATATGCTGCATATGTAGACTACCCAGCAGGTGTAAATGTTACAGTAACACCAGCTAAGCTCAAGTTTACAAATACCGGGGAAAAGATGTCTTTCTGTGTTGATTTCACCCCCCTCAAGAATAGTAATGGAAGCTTTGTGTTTGGAGCTCTGACATGGAGTAATGGTATTCAGAAGGTTAGGAGTCCTATTGGTCTTAATGTGCTTTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

863

Amino Acids

92.5

Weight (kDa)

7.04

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps4_C PF09336 12 - 72 1.3e-17 Vps4 C terminal oligomerisation domain
Inhibitor_I9 PF05922 134 - 210 4.9e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 236 - 689 4.3e-42 Subtilase family
PA PF02225 491 - 567 3.1e-07 PA domain
fn3_6 PF17766 763 - 856 4.5e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1926
AarI CACCTGC 1 cut(s) 2396
AasI GACNNNNNNGTC 2 cut(s) 194, 963
Acc36I ACCTGC 2 cut(s) 1783, 2396
AccB1I GGYRCC 3 cut(s) 417, 1940, 2093
AccBSI CCGCTC 1 cut(s) 298
AccI GTMKAC 2 cut(s) 663, 2394
AciI CCGC 3 cut(s) 62, 296, 1044
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 9 cut(s) 106, 984, 997, 1172, 1619, 1632, 2078, 2128, 2147
AcoI YGGCCR 4 cut(s) 743, 834, 1023, 1665
AcsI RAATTY 5 cut(s) 16, 794, 823, 1346, 1372
AcuI CTGAAG 2 cut(s) 459, 916
AfaI GTAC 5 cut(s) 153, 490, 1288, 1727, 2347
AfiI CCNNNNNNNGG 3 cut(s) 308, 1806, 2306
AflIII ACRYGT 1 cut(s) 1960
AgeI ACCGGT 1 cut(s) 491
AjnI CCWGG 6 cut(s) 644, 1307, 1459, 1855, 2003, 2155
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
Alw21I GWGCWC 3 cut(s) 635, 1544, 2527
Alw26I GTCTC 4 cut(s) 1216, 1544, 1887, 1894
Alw44I GTGCAC 1 cut(s) 1540
AlwI GGATC 9 cut(s) 106, 984, 997, 1172, 1619, 1632, 2078, 2128, 2147
AlwNI CAGNNNCTG 2 cut(s) 1034, 1856
Ama87I CYCGRG 1 cut(s) 942
AoxI GGCC 9 cut(s) 167, 369, 743, 834, 899, 1023, 1665, 1808, 2368
ApaLI GTGCAC 1 cut(s) 1540
ApoI RAATTY 5 cut(s) 16, 794, 823, 1346, 1372
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AsiGI ACCGGT 1 cut(s) 491
AsuC2I CCSGG 3 cut(s) 57, 1664, 2455
AsuHPI GGTGA 5 cut(s) 64, 472, 1151, 1873, 2476
AsuNHI GCTAGC 1 cut(s) 2216
AvaI CYCGRG 1 cut(s) 942
AvaII GGWCC 6 cut(s) 44, 64, 187, 779, 1172, 1853
BaeGI GKGCMC 2 cut(s) 420, 1544
BalI TGGCCA 3 cut(s) 745, 836, 1025
BamHI GGATCC 2 cut(s) 989, 1624
BanI GGYRCC 3 cut(s) 417, 1940, 2093
BanII GRGCYC 1 cut(s) 2527
Bbv12I GWGCWC 3 cut(s) 635, 1544, 2527
BccI CCATC 5 cut(s) 134, 947, 1136, 1408, 1780
BceAI ACGGC 1 cut(s) 2377
BciT130I CCWGG 6 cut(s) 646, 1309, 1461, 1857, 2005, 2157
BcnI CCSGG 3 cut(s) 57, 1664, 2455
BcoDI GTCTC 4 cut(s) 1216, 1544, 1887, 1894
BfaI CTAG 8 cut(s) 101, 159, 258, 656, 1737, 2054, 2217, 2252
BfmI CTRYAG 2 cut(s) 1968, 2203
BfuAI ACCTGC 2 cut(s) 1783, 2396
BglII AGATCT 2 cut(s) 254, 277
BlpI GCTNAGC 2 cut(s) 570, 2433
BmcAI AGTACT 1 cut(s) 1288
Bme1390I CCNGG 9 cut(s) 57, 646, 1309, 1461, 1664, 1857, 2005, 2157, 2455
Bme18I GGWCC 6 cut(s) 44, 64, 187, 779, 1172, 1853
BmeT110I CYCGRG 1 cut(s) 942
BmrFI CCNGG 9 cut(s) 57, 646, 1309, 1461, 1664, 1857, 2005, 2157, 2455
BmrI ACTGGG 4 cut(s) 1278, 1724, 2132, 2220
BmsI GCATC 5 cut(s) 937, 1093, 1120, 1195, 1268
BmtI GCTAGC 1 cut(s) 2220
BmuI ACTGGG 4 cut(s) 1278, 1724, 2132, 2220
BpmI CTGGAG 2 cut(s) 1482, 2026
Bpu1102I GCTNAGC 2 cut(s) 570, 2433
BpuEI CTTGAG 4 cut(s) 78, 926, 2423, 2477
BpuMI CCSGG 3 cut(s) 57, 1664, 2455
BsaI GGTCTC 2 cut(s) 1887, 1894
BsaJI CCNNGG 9 cut(s) 373, 535, 645, 1168, 1307, 1459, 1803, 1856, 2454
BsaWI WCCGGW 2 cut(s) 491, 1885
BsaXI ACNNNNNCTCC 6 cut(s) 795, 825, 959, 989, 2527, 2557
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 1806, 2306
Bse118I RCCGGY 2 cut(s) 482, 491
Bse1I ACTGG 5 cut(s) 820, 1284, 1730, 2138, 2215
Bse3DI GCAATG 1 cut(s) 1303
BseBI CCWGG 6 cut(s) 646, 1309, 1461, 1857, 2005, 2157
BseDI CCNNGG 9 cut(s) 373, 535, 645, 1168, 1307, 1459, 1803, 1856, 2454
BseGI GGATG 3 cut(s) 235, 1132, 1659
BseLI CCNNNNNNNGG 3 cut(s) 308, 1806, 2306
BseMI GCAATG 1 cut(s) 1303
BseMII CTCAG 4 cut(s) 558, 584, 620, 1773
BseNI ACTGG 5 cut(s) 820, 1284, 1730, 2138, 2215
BseRI GAGGAG 2 cut(s) 816, 1789
BseSI GKGCMC 2 cut(s) 420, 1544
BseYI CCCAGC 1 cut(s) 2401
BsgI GTGCAG 2 cut(s) 1068, 1761
BshFI GGCC 9 cut(s) 169, 371, 745, 836, 901, 1025, 1667, 1810, 2370
BshNI GGYRCC 3 cut(s) 417, 1940, 2093
BshTI ACCGGT 1 cut(s) 491
BsiHKAI GWGCWC 3 cut(s) 635, 1544, 2527
BsiHKCI CYCGRG 1 cut(s) 942
BsiSI CCGG 6 cut(s) 56, 483, 492, 1664, 1886, 2454
BslFI GGGAC 2 cut(s) 200, 1144
BslI CCNNNNNNNGG 3 cut(s) 308, 1806, 2306
BsmAI GTCTC 4 cut(s) 1216, 1544, 1887, 1894
BsmFI GGGAC 2 cut(s) 200, 1144
BsmI GAATGC 2 cut(s) 1232, 1483
BsnI GGCC 9 cut(s) 169, 371, 745, 836, 901, 1025, 1667, 1810, 2370
Bso31I GGTCTC 2 cut(s) 1887, 1894
BsoBI CYCGRG 1 cut(s) 942
Bsp1286I GDGCHC 4 cut(s) 420, 635, 1544, 2527
Bsp1720I GCTNAGC 2 cut(s) 570, 2433
Bsp19I CCATGG 2 cut(s) 373, 1803
BspACI CCGC 3 cut(s) 62, 296, 1044
BspANI GGCC 9 cut(s) 169, 371, 745, 836, 901, 1025, 1667, 1810, 2370
BspCNI CTCAG 4 cut(s) 557, 583, 621, 1774
BspMAI CTGCAG 2 cut(s) 1972, 2207
BspMI ACCTGC 2 cut(s) 1783, 2396
BspOI GCTAGC 1 cut(s) 2220
BspPI GGATC 9 cut(s) 106, 984, 997, 1172, 1619, 1632, 2078, 2128, 2147
BspT107I GGYRCC 3 cut(s) 417, 1940, 2093
BspTNI GGTCTC 2 cut(s) 1887, 1894
BsrBI CCGCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 1303
BsrFI RCCGGY 2 cut(s) 482, 491
BsrI ACTGG 5 cut(s) 820, 1284, 1730, 2138, 2215
BssAI RCCGGY 2 cut(s) 482, 491
BssECI CCNNGG 9 cut(s) 373, 535, 645, 1168, 1307, 1459, 1803, 1856, 2454
BssT1I CCWWGG 4 cut(s) 373, 535, 1168, 1803
Bst2UI CCWGG 6 cut(s) 646, 1309, 1461, 1857, 2005, 2157
Bst4CI ACNGT 8 cut(s) 175, 524, 734, 997, 1430, 2350, 2377, 2422
Bst6I CTCTTC 1 cut(s) 927
BstAPI GCANNNNNTGC 4 cut(s) 338, 1055, 1448, 1478
BstC8I GCNNGC 6 cut(s) 789, 1294, 1330, 1483, 1513, 2218
BstDEI CTNAG 6 cut(s) 544, 570, 629, 1782, 1870, 2433
BstDSI CCRYGG 2 cut(s) 373, 1803
BstF5I GGATG 3 cut(s) 235, 1132, 1659
BstMAI GTCTC 4 cut(s) 1216, 1544, 1887, 1894
BstNI CCWGG 6 cut(s) 646, 1309, 1461, 1857, 2005, 2157
BstNSI RCATGY 3 cut(s) 1083, 1296, 1964
BstSCI CCNGG 9 cut(s) 55, 644, 1307, 1459, 1662, 1855, 2003, 2155, 2453
BstSFI CTRYAG 2 cut(s) 1968, 2203
BstSLI GKGCMC 2 cut(s) 420, 1544
BstX2I RGATCY 7 cut(s) 111, 254, 277, 989, 1624, 2083, 2120
BstYI RGATCY 7 cut(s) 111, 254, 277, 989, 1624, 2083, 2120
BsuRI GGCC 9 cut(s) 169, 371, 745, 836, 901, 1025, 1667, 1810, 2370
BtgI CCRYGG 2 cut(s) 373, 1803
BtsCI GGATG 3 cut(s) 235, 1132, 1659
BtsI GCAGTG 3 cut(s) 381, 969, 1797
BtsIMutI CAGTG 5 cut(s) 381, 739, 969, 1797, 2208
BveI ACCTGC 2 cut(s) 1783, 2396
Cac8I GCNNGC 6 cut(s) 789, 1294, 1330, 1483, 1513, 2218
CaiI CAGNNNCTG 2 cut(s) 1034, 1856
Cfr10I RCCGGY 2 cut(s) 482, 491
CseI GACGC 1 cut(s) 674
Csp6I GTAC 5 cut(s) 152, 489, 1287, 1726, 2346
CspAI ACCGGT 1 cut(s) 491
CspCI CAANNNNNGTGG 4 cut(s) 1657, 1692, 2092, 2127
CviQI GTAC 5 cut(s) 152, 489, 1287, 1726, 2346
DdeI CTNAG 6 cut(s) 544, 570, 629, 1782, 1870, 2433
DraI TTTAAA 1 cut(s) 1401
DrdI GACNNNNNNGTC 2 cut(s) 194, 963
DseDI GACNNNNNNGTC 2 cut(s) 194, 963
EaeI YGGCCR 4 cut(s) 743, 834, 1023, 1665
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
Ecl136II GAGCTC 1 cut(s) 2525
Eco130I CCWWGG 4 cut(s) 373, 535, 1168, 1803
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 2527
Eco31I GGTCTC 2 cut(s) 1887, 1894
Eco47I GGWCC 6 cut(s) 44, 64, 187, 779, 1172, 1853
Eco53kI GAGCTC 1 cut(s) 2525
Eco57I CTGAAG 2 cut(s) 459, 916
Eco88I CYCGRG 1 cut(s) 942
EcoICRI GAGCTC 1 cut(s) 2525
EcoO109I RGGNCCY 3 cut(s) 187, 779, 1853
EcoRI GAATTC 2 cut(s) 16, 1346
EcoRII CCWGG 6 cut(s) 644, 1307, 1459, 1855, 2003, 2155
EcoT14I CCWWGG 4 cut(s) 373, 535, 1168, 1803
EcoT22I ATGCAT 1 cut(s) 1234
EcoT38I GRGCYC 1 cut(s) 2527
ErhI CCWWGG 4 cut(s) 373, 535, 1168, 1803
FalI AAGNNNNNCTT 6 cut(s) 174, 206, 495, 527, 1233, 1265
FaqI GGGAC 2 cut(s) 200, 1144
FauNDI CATATG 1 cut(s) 2389
FblI GTMKAC 2 cut(s) 663, 2394
FokI GGATG 3 cut(s) 242, 1139, 1646
FriOI GRGCYC 1 cut(s) 2527
FspBI CTAG 8 cut(s) 101, 159, 258, 656, 1737, 2054, 2217, 2252
GsaI CCCAGC 1 cut(s) 2405
GsuI CTGGAG 2 cut(s) 1482, 2026
HaeIII GGCC 9 cut(s) 169, 371, 745, 836, 901, 1025, 1667, 1810, 2370
HapII CCGG 6 cut(s) 56, 483, 492, 1664, 1886, 2454
HgaI GACGC 1 cut(s) 674
HindIII AAGCTT 4 cut(s) 551, 1483, 1513, 2509
HpaII CCGG 6 cut(s) 56, 483, 492, 1664, 1886, 2454
HphI GGTGA 5 cut(s) 64, 472, 1151, 1873, 2476
Hpy166II GTNNAC 8 cut(s) 44, 409, 497, 664, 1542, 1958, 2395, 2445
Hpy188III TCNNGA 9 cut(s) 13, 944, 1124, 1175, 1237, 1682, 2054, 2124, 2494
Hpy8I GTNNAC 8 cut(s) 44, 409, 497, 664, 1542, 1958, 2395, 2445
HpyCH4III ACNGT 8 cut(s) 175, 524, 734, 997, 1430, 2350, 2377, 2422
HpyCH4IV ACGT 1 cut(s) 1359
HpyF3I CTNAG 6 cut(s) 544, 570, 629, 1782, 1870, 2433
HpySE526I ACGT 1 cut(s) 1359
LmnI GCTCC 5 cut(s) 303, 377, 1220, 1309, 2522
LweI GCATC 5 cut(s) 937, 1093, 1120, 1195, 1268
MaeI CTAG 8 cut(s) 101, 159, 258, 656, 1737, 2054, 2217, 2252
MaeII ACGT 1 cut(s) 1359
MaeIII GTNAC 8 cut(s) 478, 811, 965, 997, 1465, 2350, 2416, 2422
MbiI CCGCTC 1 cut(s) 298
MboII GAAGA 5 cut(s) 202, 914, 922, 1214, 1697
MfeI CAATTG 1 cut(s) 2148
MflI RGATCY 7 cut(s) 111, 254, 277, 989, 1624, 2083, 2120
MhlI GDGCHC 4 cut(s) 420, 635, 1544, 2527
MlsI TGGCCA 3 cut(s) 745, 836, 1025
MluNI TGGCCA 3 cut(s) 745, 836, 1025
MlyI GAGTC 7 cut(s) 277, 758, 917, 973, 1473, 2017, 2569
Mox20I TGGCCA 3 cut(s) 745, 836, 1025
Mph1103I ATGCAT 1 cut(s) 1234
MscI TGGCCA 3 cut(s) 745, 836, 1025
MseI TTAA 7 cut(s) 513, 1400, 1517, 1521, 2133, 2292, 2574
MslI CAYNNNNRTG 2 cut(s) 1946, 2309
Msp20I TGGCCA 3 cut(s) 745, 836, 1025
MspA1I CMGCKG 1 cut(s) 1454
MspI CCGG 6 cut(s) 56, 483, 492, 1664, 1886, 2454
MspR9I CCNGG 9 cut(s) 57, 646, 1309, 1461, 1664, 1857, 2005, 2157, 2455
MunI CAATTG 1 cut(s) 2148
Mva1269I GAATGC 2 cut(s) 1232, 1483
MvaI CCWGG 6 cut(s) 646, 1309, 1461, 1857, 2005, 2157
NciI CCSGG 3 cut(s) 57, 1664, 2455
NcoI CCATGG 2 cut(s) 373, 1803
NdeI CATATG 1 cut(s) 2389
NheI GCTAGC 1 cut(s) 2216
NmuCI GTSAC 3 cut(s) 478, 965, 1465
NsiI ATGCAT 1 cut(s) 1234
NspI RCATGY 3 cut(s) 1083, 1296, 1964
PacI TTAATTAA 1 cut(s) 1521
PaeI GCATGC 1 cut(s) 1296
PaeR7I CTCGAG 1 cut(s) 942
PaqCI CACCTGC 1 cut(s) 2396
PceI AGGCCT 1 cut(s) 169
PciI ACATGT 1 cut(s) 1960
PctI GAATGC 2 cut(s) 1232, 1483
PfeI GAWTC 7 cut(s) 241, 440, 466, 1312, 1615, 1740, 2177
PfoI TCCNGGA 2 cut(s) 2003, 2155
PinAI ACCGGT 1 cut(s) 491
PleI GAGTC 7 cut(s) 276, 757, 916, 972, 1472, 2016, 2568
PpsI GAGTC 7 cut(s) 276, 757, 916, 972, 1472, 2016, 2568
PpuMI RGGWCCY 3 cut(s) 187, 779, 1853
PscI ACATGT 1 cut(s) 1960
PsiI TTATAA 1 cut(s) 1926
Psp124BI GAGCTC 1 cut(s) 2527
Psp1406I AACGTT 1 cut(s) 1359
Psp5II RGGWCCY 3 cut(s) 187, 779, 1853
Psp6I CCWGG 6 cut(s) 644, 1307, 1459, 1855, 2003, 2155
PspFI CCCAGC 1 cut(s) 2401
PspGI CCWGG 6 cut(s) 644, 1307, 1459, 1855, 2003, 2155
PspPPI RGGWCCY 3 cut(s) 187, 779, 1853
PstI CTGCAG 2 cut(s) 1972, 2207
PstNI CAGNNNCTG 2 cut(s) 1034, 1856
PsuI RGATCY 7 cut(s) 111, 254, 277, 989, 1624, 2083, 2120
PvuII CAGCTG 1 cut(s) 1454
RsaI GTAC 5 cut(s) 153, 490, 1288, 1727, 2347
RsaNI GTAC 5 cut(s) 152, 489, 1287, 1726, 2346
RseI CAYNNNNRTG 2 cut(s) 1946, 2309
SacI GAGCTC 1 cut(s) 2527
SaqAI TTAA 7 cut(s) 513, 1400, 1517, 1521, 2133, 2292, 2574
ScaI AGTACT 1 cut(s) 1288
SchI GAGTC 7 cut(s) 277, 758, 917, 973, 1473, 2017, 2569
ScrFI CCNGG 9 cut(s) 57, 646, 1309, 1461, 1664, 1857, 2005, 2157, 2455
SduI GDGCHC 4 cut(s) 420, 635, 1544, 2527
SfaNI GCATC 5 cut(s) 937, 1093, 1120, 1195, 1268
SfcI CTRYAG 2 cut(s) 1968, 2203
Sfr274I CTCGAG 1 cut(s) 942
SinI GGWCC 6 cut(s) 44, 64, 187, 779, 1172, 1853
SlaI CTCGAG 1 cut(s) 942
SmiMI CAYNNNNRTG 2 cut(s) 1946, 2309
SmlI CTYRAG 5 cut(s) 93, 905, 942, 2438, 2492
SmoI CTYRAG 5 cut(s) 93, 905, 942, 2438, 2492
SphI GCATGC 1 cut(s) 1296
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 3 cut(s) 62, 296, 1044
SspMI CTAG 8 cut(s) 101, 159, 258, 656, 1737, 2054, 2217, 2252
SstI GAGCTC 1 cut(s) 2527
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 9 cut(s) 55, 644, 1307, 1459, 1662, 1855, 2003, 2155, 2453
StyI CCWWGG 4 cut(s) 373, 535, 1168, 1803
TaaI ACNGT 8 cut(s) 175, 524, 734, 997, 1430, 2350, 2377, 2422
TaiI ACGT 1 cut(s) 1362
TaqI TCGA 4 cut(s) 943, 1149, 1946, 2193
TatI WGTACW 1 cut(s) 1286
TfiI GAWTC 7 cut(s) 241, 440, 466, 1312, 1615, 1740, 2177
Tru1I TTAA 7 cut(s) 513, 1400, 1517, 1521, 2133, 2292, 2574
Tru9I TTAA 7 cut(s) 513, 1400, 1517, 1521, 2133, 2292, 2574
TscAI CASTG 5 cut(s) 388, 739, 976, 1797, 2215
TseFI GTSAC 3 cut(s) 478, 965, 1465
Tsp45I GTSAC 3 cut(s) 478, 965, 1465
TspDTI ATGAA 3 cut(s) 22, 216, 783
TspGWI ACGGA 1 cut(s) 2133
TspRI CASTG 5 cut(s) 388, 739, 976, 1797, 2215
VneI GTGCAC 1 cut(s) 1540
VpaK11BI GGWCC 6 cut(s) 44, 64, 187, 779, 1172, 1853
XapI RAATTY 5 cut(s) 16, 794, 823, 1346, 1372
XbaI TCTAGA 1 cut(s) 2053
XceI RCATGY 3 cut(s) 1083, 1296, 1964
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 942
XmiI GTMKAC 2 cut(s) 663, 2394
XspI CTAG 8 cut(s) 101, 159, 258, 656, 1737, 2054, 2217, 2252
ZrmI AGTACT 1 cut(s) 1288
Zsp2I ATGCAT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.