RLG00000034247

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002713
Physical Location & Seq
Reverse (-)
39338876 .. 39349236
10361 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034247

Sequence Viewer

Length: 3531 bp
ATGTATAGCAATTTCAAAGAGCAGGCTATAGAGTACGTGAAGCAAGCCGTACATGAAGACAACAATGGAAACTACGCAAAGGCCTTTCCTTTGTACATGAACGCTCTCGAGTACTTCAAGACCCACCTCAAGTACGAGAAGAACCCTAAGATCAAGGAGGCTATTACCCAGAAATTCACCGAGTATTTGCGCCGGGCCGAGGAGATTCGGGCTGTTTTGGATGACGGCGGGCCCGGCCCGGCCTCCAACGGCGACGCGGCGGTCGCGACCAGGCCCAAGACCAAGCCCAAGGACGGGGAAGGCGGCGACGGGGAGGATCCGGAGCAGGCCAAGCTCCGGGCTGGGCTTAATTCGGCGATTATTAGGGAAAAGCCCAACGTCAAGTGGAATGATGTGGCGGGTCTAGAGAGCGCTAAGCAGGCTTTGCAAGAGGCGGTGATATTGCCGGTCAAGTTCCCTCAGTTCTTTACTGGCAAGAGACGACCATGGAGGGCTTTTCTATTGTATGGTCCTCCTGGAACTGGAAAGTCATACTTGGCCAAGGCTGTAGCAACTGAAGCAGACTCCACATTTTTCAGTATTTCCTCTTCAGACCTGGTTTCAAAGTGGATGGGTGAGAGTGAGAAGCTAGTTTCAAACCTCTTCCAAATGGCTCGAGATAGTGCCCCATCCATCATCTTCGTTGATGAAATAGATTCCTTATGTGGGACGCGTGGAGAAGGCAATGAGAGTGAAGCTTCTAGACGAATCAAGACAGAACTTCTTGTGCAGATGCAGGGTGTGGGAACCAATGACCAAAAAGTTCTTGTTCTTGCAGCGACAAATACTCCCTATGCATTAGACCAGGCCATTCGAAGACGTTTTGACAAGCGTATATACATTCCACTTCCAGATGTAAAGGCTCGACAGCATATGTTCAAAGTCCACTTAGGAGATACTCCTCACAACTTGACAGAAAGTGATTTTGAAAGCTTAGCTCACAAAACTGAGGGATTTTCGGGTTCAGATATTTCTGTTTGTGTCAAGGATGTTCTTTTTGAACCTGTACGCAAAACACAAGATGCCATGTTCTTTTTCAAAAATTCTGATAATATGTTCATACCTTGTGGACCAAAGCAACCGGGTGCCGTCCAAATCACCATGCAGGAGTTGGCAGCTCAAGGACTAGCTTCACAGATCCTTCCACCTCCTATCACAAAAACAGATTTTGATAAGGTACTTGCTAGACAGAGGCCTACTGTGAGCAAAGGTGACCTTGAAGTTCATGAAAGAGTCCTCACTCTTCTCTTGTTCCTTAGACTTTCTTTGGCCCATGGAGCCACTCCTAAAACAAAGCACTATATTGTTTACATGGGGCACCCCTCGCACTCCAACTCGGATTCTGTCATCAGAGCTAACCATGAGATTCTTGCTTCAGTCACTGGCAGCACTGGTGGACAAGAAGCAGCAGTTAACCATTACAGTAAAAGTTTCCAAGGCTTCTCAGCCAAGCTTACACCAGATCAAGCTCAACAACTAGCTGAACATGATTCGGTTATTTCGGTGTTTGAGAGCAAGACAAACAAACTGAGCACAACACGTTCTTGGGATTTTCTAGGCGTAGACGCTACTCCTAATTACAATCAAATGCCAATGGGTTCCAAATCCAATGTCATTGTTGGTGTAATTGACACTGGAGTTTGGCCAGAGTCAGAGAGCTTCAATGATGAAGGATTAGGACCTGTGCCAGCTAAATTCAGAGGAGAGTGTGTTACTGGTCAGAATTTTACATTGGCCAACTGCAACAGAAAAATCATAGGCTCTCGCTTCTATTATCAAGGATTTGAAGTAGAAAAAGGGCCACTTGAGTCGTTTGCACCTCTTCCTTTCTTCAGATCAGCTCGAGATGCTGATGGACATGGGAGTCACACTGCCTCCACGATAGCTGGATCCACAGTAACTAATGTCAGCTTCTTGGGAATGGCCACAGGAACTGCAAGAGGCGGTGCAAAAAGTGCCAGACTTGCTATCTACAAGGCATGTTGGTTCGACTTGTGTAGTGATGCAGACCTTCTTTCAGCTCTGGATGATGCCATTGGTGATGGTGTCGATATTCTGTCCCTTTCCCTTGGCCCTGATCCTCCACAGCCAACATATTTTGAAGATGCAGTCTCCATTGGAGCTTTCCATGCATTCCAGAAAGGAATACTTGTTTCTGCATCAGCAGGGAACTCTGGTTTCCCTAGTACTGCATGCAATGTAGCTCCCTGGATTCTCACTGTTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTTTATCTGGGAAATTCACAAATACTAAAGGGTTCCTCTTTAAACCCACTGAAGATGGATAATTCTTACAGTTTGATTGCTGCAAGTGTTGCAGCTGCCCCTGGAGTCACAGCAAAGAATGCCAGCTTCTGCAAGGACAATACTCTAAATGCAAGCTTAATTAAGGGAAAAATCGTTGTGTGCACCTTTGAGACATTTACCGACAACCGAAGGGAAAAAAGTATAGTTGTAAGACAAGGTGGTGGTGTAGGAATGATTCTTGTGGATCCATTTCTCAAAGATGTTGGCTTTGAGTTTGTCATCCCGGCCACCCTAATTGGTCAAGAAGAAGCTGCACAGCTTCAAGCATACATGACAACAGAGAAGTACCCAGTTGCTAGAATCTCACAAACGACAACAATTCTGAAAACTAAACCTGCACCTGAGATGGCAGTGTTCTCCTCCATGGGGCCAAATATCATAACCCCAGACATTATCAAACCAGATATTACAGGACCTGGGGTGAATATCTTAGCAGCATGGTCTCCGGTCTCAACTACACCCACAGCAGAAAGGTCTGTCAATTATAACATCATTTCTGGCACCTCGATGTCTTGTCCACATGTATCTGCAGTTGCAGCAACTTTGAAATCATACCAACCTTCCTGGAGTCCAGCAGCCATAATGTCTGCAATAATGACAACAGCAACAGTTCTAGACAACAACAAACGCCCTATTGGGAGAGATCCAAATGGCACCCCTACCACACCTTTTGACTACGGATCTGGACACATTAACCCAGTTGCCGCAATTGATCCTGGACTTGTATATGATTTTGATTCCCATGACATAATCGATTTTCTCTGCAGTACTGGGGCTAGCACTTTGCAACTGAAAAACCTCACGGGTAATCTAGTTTATTGCCAGAAGCCTTCTATACCTTCCTACAATTTTAACTACCCTTCCATTGGTGTGTCAAAAATGAGTGGAAGGCTATCTGTTCAGCGTACAGTTACTTATTATGGCAGAGGCCCAACGGTATATGCTGCATATGTAGACTACCCGGCAGGTGTAAATGTTACAGTAAGACCAGCTAAACTCAAGTTTACAAATACCGGGGAAAAGATGTCTTTCTGTGTTGATTTCACCCCCCTCAAGAATAGTAATGGAACCTTTGTGTTTGGAGCTCTGACATGGAGTAATGGTATTCAGAATGTTAGGAGTCCTATTGGTCTTAATGTGCTTTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1177

Amino Acids

127.38

Weight (kDa)

7.28

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIT PF04212 7 - 70 8.6e-21 MIT (microtubule interacting and transport) domain
AAA PF00004 167 - 296 1.2e-42 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 321 - 354 4.1e-06 AAA+ lid domain
Vps4_C PF09336 367 - 423 2.8e-16 Vps4 C terminal oligomerisation domain
Inhibitor_I9 PF05922 447 - 523 6.7e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 549 - 1020 3.4e-42 Subtilase family
PA PF02225 804 - 880 3.3e-07 PA domain
fn3_6 PF17766 1076 - 1169 1.8e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2865
AarI CACCTGC 1 cut(s) 3335
AasI GACNNNNNNGTC 2 cut(s) 260, 1902
Acc36I ACCTGC 2 cut(s) 2722, 3335
AccB1I GGYRCC 4 cut(s) 1124, 1356, 2879, 3032
AccI GTMKAC 2 cut(s) 1602, 3333
AccII CGCG 3 cut(s) 257, 266, 712
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 8 cut(s) 228, 257, 260, 303, 398, 434, 1983, 3084
AclI AACGTT 1 cut(s) 2298
AcoI YGGCCR 5 cut(s) 537, 1682, 1773, 1962, 2604
AcsI RAATTY 6 cut(s) 173, 1081, 1733, 1762, 2285, 2311
AcuI CTGAAG 5 cut(s) 573, 576, 1398, 1855, 2369
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 8 cut(s) 199, 293, 294, 336, 521, 705, 2745, 3245
AflIII ACRYGT 3 cut(s) 710, 1577, 2899
AjnI CCWGG 9 cut(s) 269, 514, 594, 843, 2246, 2398, 2794, 2942, 3094
AjuI GAANNNNNNNTTGG 2 cut(s) 1754, 1786
Alw21I GWGCWC 3 cut(s) 1574, 2483, 3466
Alw26I GTCTC 5 cut(s) 472, 2155, 2483, 2826, 2833
Alw44I GTGCAC 1 cut(s) 2479
AlwNI CAGNNNCTG 4 cut(s) 1421, 1973, 2427, 2795
Ama87I CYCGRG 3 cut(s) 107, 654, 1881
Aor13HI TCCGGA 1 cut(s) 319
Aor51HI AGCGCT 1 cut(s) 412
ApaI GGGCCC 1 cut(s) 234
ApaLI GTGCAC 1 cut(s) 2479
ApoI RAATTY 6 cut(s) 173, 1081, 1733, 1762, 2285, 2311
ArsI GACNNNNNNTTYG 2 cut(s) 1635, 1667
AspLEI GCGC 2 cut(s) 192, 413
AsuC2I CCSGG 8 cut(s) 194, 234, 239, 338, 1122, 2603, 3341, 3394
AsuHPI GGTGA 8 cut(s) 169, 448, 626, 1129, 1262, 2090, 2812, 3415
AsuII TTCGAA 1 cut(s) 853
AsuNHI GCTAGC 1 cut(s) 3155
AvaI CYCGRG 3 cut(s) 107, 654, 1881
AvaII GGWCC 4 cut(s) 509, 1109, 1718, 2792
BaeGI GKGCMC 4 cut(s) 234, 667, 1359, 2483
BalI TGGCCA 4 cut(s) 539, 1684, 1775, 1964
BamHI GGATCC 3 cut(s) 316, 1928, 2563
BanI GGYRCC 4 cut(s) 1124, 1356, 2879, 3032
BanII GRGCYC 2 cut(s) 234, 3466
BarI GAAGNNNNNNTAC 2 cut(s) 571, 603
BbsI GAAGAC 2 cut(s) 63, 862
Bbv12I GWGCWC 3 cut(s) 1574, 2483, 3466
BccI CCATC 7 cut(s) 604, 676, 680, 1886, 2075, 2347, 2719
BceAI ACGGC 4 cut(s) 32, 241, 265, 1112
BciT130I CCWGG 9 cut(s) 271, 516, 596, 845, 2248, 2400, 2796, 2944, 3096
BcnI CCSGG 8 cut(s) 194, 234, 239, 338, 1122, 2603, 3341, 3394
BcoDI GTCTC 5 cut(s) 472, 2155, 2483, 2826, 2833
BfmI CTRYAG 4 cut(s) 27, 546, 2907, 3142
BfoI RGCGCY 1 cut(s) 414
BfuAI ACCTGC 2 cut(s) 2722, 3335
BlpI GCTNAGC 2 cut(s) 414, 973
BmcAI AGTACT 3 cut(s) 113, 2227, 3148
Bme18I GGWCC 4 cut(s) 509, 1109, 1718, 2792
BmeT110I CYCGRG 3 cut(s) 107, 654, 1881
BmrI ACTGGG 3 cut(s) 2663, 3071, 3159
BmsI GCATC 7 cut(s) 762, 1051, 1876, 2032, 2059, 2134, 2207
BmtI GCTAGC 1 cut(s) 3159
BmuI ACTGGG 3 cut(s) 2663, 3071, 3159
BpiI GAAGAC 2 cut(s) 63, 862
BpmI CTGGAG 3 cut(s) 1695, 2421, 2965
Bpu1102I GCTNAGC 2 cut(s) 414, 973
Bpu14I TTCGAA 1 cut(s) 853
BpuEI CTTGAG 5 cut(s) 113, 1143, 1865, 3362, 3416
BpuMI CCSGG 8 cut(s) 194, 234, 239, 338, 1122, 2603, 3341, 3394
Bsa29I ATCGAT 1 cut(s) 3132
BsaAI YACGTR 1 cut(s) 37
BsaI GGTCTC 2 cut(s) 2826, 2833
BsaWI WCCGGW 2 cut(s) 319, 2824
BsaXI ACNNNNNCTCC 8 cut(s) 811, 841, 1734, 1764, 1898, 1928, 3466, 3496
Bsc4I CCNNNNNNNGG 8 cut(s) 199, 293, 294, 336, 521, 705, 2745, 3245
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 9 cut(s) 475, 526, 1426, 1435, 1678, 1759, 2669, 3077, 3154
Bse3DI GCAATG 2 cut(s) 730, 2242
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 9 cut(s) 271, 516, 596, 845, 2248, 2400, 2796, 2944, 3096
BseCI ATCGAT 1 cut(s) 3132
BseGI GGATG 6 cut(s) 226, 615, 668, 1033, 2071, 2598
BseLI CCNNNNNNNGG 8 cut(s) 199, 293, 294, 336, 521, 705, 2745, 3245
BseMI GCAATG 2 cut(s) 730, 2242
BseMII CTCAG 5 cut(s) 473, 978, 1497, 1559, 2712
BseNI ACTGG 9 cut(s) 475, 526, 1426, 1435, 1678, 1759, 2669, 3077, 3154
BseRI GAGGAG 4 cut(s) 215, 930, 1755, 2728
BseSI GKGCMC 4 cut(s) 234, 667, 1359, 2483
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 3 cut(s) 788, 2616, 2700
Bsh1236I CGCG 3 cut(s) 257, 266, 712
Bsh1285I CGRYCG 1 cut(s) 264
BshNI GGYRCC 4 cut(s) 1124, 1356, 2879, 3032
BshVI ATCGAT 1 cut(s) 3132
BsiEI CGRYCG 1 cut(s) 264
BsiHKAI GWGCWC 3 cut(s) 1574, 2483, 3466
BsiHKCI CYCGRG 3 cut(s) 107, 654, 1881
BslFI GGGAC 2 cut(s) 721, 2083
BslI CCNNNNNNNGG 8 cut(s) 199, 293, 294, 336, 521, 705, 2745, 3245
BsmAI GTCTC 5 cut(s) 472, 2155, 2483, 2826, 2833
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 2 cut(s) 721, 2083
BsmI GAATGC 2 cut(s) 2171, 2422
Bso31I GGTCTC 2 cut(s) 2826, 2833
BsoBI CYCGRG 3 cut(s) 107, 654, 1881
Bsp119I TTCGAA 1 cut(s) 853
Bsp120I GGGCCC 1 cut(s) 230
Bsp1286I GDGCHC 6 cut(s) 234, 667, 1359, 1574, 2483, 3466
Bsp13I TCCGGA 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 93
Bsp1720I GCTNAGC 2 cut(s) 414, 973
Bsp19I CCATGG 3 cut(s) 485, 1312, 2742
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 8 cut(s) 228, 257, 260, 303, 398, 434, 1983, 3084
BspCNI CTCAG 5 cut(s) 472, 979, 1496, 1560, 2713
BspDI ATCGAT 1 cut(s) 3132
BspEI TCCGGA 1 cut(s) 319
BspFNI CGCG 3 cut(s) 257, 266, 712
BspHI TCATGA 1 cut(s) 1264
BspMAI CTGCAG 2 cut(s) 2911, 3146
BspMI ACCTGC 2 cut(s) 2722, 3335
BspOI GCTAGC 1 cut(s) 3159
BspT104I TTCGAA 1 cut(s) 853
BspT107I GGYRCC 4 cut(s) 1124, 1356, 2879, 3032
BspTNI GGTCTC 2 cut(s) 2826, 2833
BsrDI GCAATG 2 cut(s) 730, 2242
BsrFI RCCGGY 1 cut(s) 445
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 9 cut(s) 475, 526, 1426, 1435, 1678, 1759, 2669, 3077, 3154
BssAI RCCGGY 1 cut(s) 445
BssT1I CCWWGG 7 cut(s) 288, 485, 540, 1312, 1474, 2107, 2742
Bst2UI CCWGG 9 cut(s) 271, 516, 596, 845, 2248, 2400, 2796, 2944, 3096
Bst4CI ACNGT 9 cut(s) 1240, 1463, 1936, 2260, 2369, 2989, 3289, 3316, 3361
Bst6I CTCTTC 4 cut(s) 592, 647, 1287, 1866
BstAPI GCANNNNNTGC 4 cut(s) 424, 1994, 2387, 2417
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 853
BstDSI CCRYGG 3 cut(s) 485, 1312, 2742
BstEII GGTNACC 1 cut(s) 1250
BstF5I GGATG 6 cut(s) 226, 615, 668, 1033, 2071, 2598
BstFNI CGCG 3 cut(s) 257, 266, 712
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 2 cut(s) 192, 413
BstMAI GTCTC 5 cut(s) 472, 2155, 2483, 2826, 2833
BstMCI CGRYCG 1 cut(s) 264
BstNI CCWGG 9 cut(s) 271, 516, 596, 845, 2248, 2400, 2796, 2944, 3096
BstNSI RCATGY 3 cut(s) 2022, 2235, 2903
BstPI GGTNACC 1 cut(s) 1250
BstSFI CTRYAG 4 cut(s) 27, 546, 2907, 3142
BstSLI GKGCMC 4 cut(s) 234, 667, 1359, 2483
BstUI CGCG 3 cut(s) 257, 266, 712
BstV2I GAAGAC 2 cut(s) 63, 862
BstX2I RGATCY 6 cut(s) 316, 1176, 1928, 2563, 3022, 3059
BstYI RGATCY 6 cut(s) 316, 1176, 1928, 2563, 3022, 3059
Bsu15I ATCGAT 1 cut(s) 3132
BsuTUI ATCGAT 1 cut(s) 3132
BtgI CCRYGG 3 cut(s) 485, 1312, 2742
BtsCI GGATG 6 cut(s) 226, 615, 668, 1033, 2071, 2598
BtsI GCAGTG 2 cut(s) 1908, 2736
BtsIMutI CAGTG 7 cut(s) 1419, 1428, 1671, 1908, 2256, 2345, 2736
BtuMI TCGCGA 1 cut(s) 266
BveI ACCTGC 2 cut(s) 2722, 3335
CaiI CAGNNNCTG 4 cut(s) 1421, 1973, 2427, 2795
CciI TCATGA 1 cut(s) 1264
CfoI GCGC 2 cut(s) 192, 413
Cfr10I RCCGGY 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 3132
CseI GACGC 3 cut(s) 263, 718, 1613
CsiI ACCWGGT 1 cut(s) 594
CspCI CAANNNNNGTGG 4 cut(s) 2596, 2631, 3031, 3066
DraI TTTAAA 1 cut(s) 2340
DrdI GACNNNNNNGTC 2 cut(s) 260, 1902
DseDI GACNNNNNNGTC 2 cut(s) 260, 1902
EaeI YGGCCR 5 cut(s) 537, 1682, 1773, 1962, 2604
Eam1104I CTCTTC 4 cut(s) 592, 647, 1287, 1866
EarI CTCTTC 4 cut(s) 592, 647, 1287, 1866
Ecl136II GAGCTC 1 cut(s) 3464
Eco130I CCWWGG 7 cut(s) 288, 485, 540, 1312, 1474, 2107, 2742
Eco147I AGGCCT 2 cut(s) 83, 1234
Eco24I GRGCYC 2 cut(s) 234, 3466
Eco31I GGTCTC 2 cut(s) 2826, 2833
Eco47I GGWCC 4 cut(s) 509, 1109, 1718, 2792
Eco47III AGCGCT 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 3464
Eco57I CTGAAG 5 cut(s) 573, 576, 1398, 1855, 2369
Eco88I CYCGRG 3 cut(s) 107, 654, 1881
Eco91I GGTNACC 1 cut(s) 1250
EcoICRI GAGCTC 1 cut(s) 3464
EcoO109I RGGNCCY 2 cut(s) 1718, 2792
EcoO65I GGTNACC 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 2285
EcoRII CCWGG 9 cut(s) 269, 514, 594, 843, 2246, 2398, 2794, 2942, 3094
EcoT14I CCWWGG 7 cut(s) 288, 485, 540, 1312, 1474, 2107, 2742
EcoT22I ATGCAT 2 cut(s) 838, 2173
EcoT38I GRGCYC 2 cut(s) 234, 3466
ErhI CCWWGG 7 cut(s) 288, 485, 540, 1312, 1474, 2107, 2742
Esp3I CGTCTC 1 cut(s) 472
FalI AAGNNNNNCTT 8 cut(s) 518, 550, 1239, 1271, 1827, 1859, 2172, 2204
FaqI GGGAC 2 cut(s) 721, 2083
FauI CCCGC 2 cut(s) 221, 391
FauNDI CATATG 2 cut(s) 912, 3328
FblI GTMKAC 2 cut(s) 1602, 3333
FokI GGATG 6 cut(s) 233, 622, 655, 1040, 2078, 2585
FriOI GRGCYC 2 cut(s) 234, 3466
GlaI GCGC 2 cut(s) 191, 412
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 3 cut(s) 1695, 2421, 2965
HaeII RGCGCY 1 cut(s) 414
HgaI GACGC 3 cut(s) 263, 718, 1613
HhaI GCGC 2 cut(s) 192, 413
Hin6I GCGC 2 cut(s) 190, 411
HinP1I GCGC 2 cut(s) 190, 411
HincII GTYRAC 1 cut(s) 1453
HindII GTYRAC 1 cut(s) 1453
HindIII AAGCTT 4 cut(s) 735, 970, 1490, 2452
HpaI GTTAAC 1 cut(s) 1453
HphI GGTGA 8 cut(s) 169, 448, 626, 1129, 1262, 2090, 2812, 3415
Hpy99I CGWCG 2 cut(s) 257, 311
HpyCH4III ACNGT 9 cut(s) 1240, 1463, 1936, 2260, 2369, 2989, 3289, 3316, 3361
HpyCH4IV ACGT 5 cut(s) 36, 378, 859, 1579, 2298
HpySE526I ACGT 5 cut(s) 36, 378, 859, 1579, 2298
HspAI GCGC 2 cut(s) 190, 411
Kpn2I TCCGGA 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 1453
LmnI GCTCC 6 cut(s) 322, 339, 1316, 2159, 2248, 3461
LweI GCATC 7 cut(s) 762, 1051, 1876, 2032, 2059, 2134, 2207
MabI ACCWGGT 1 cut(s) 594
MaeII ACGT 5 cut(s) 36, 378, 859, 1579, 2298
MaeIII GTNAC 8 cut(s) 1250, 1417, 1750, 1904, 1936, 2404, 3289, 3355
MfeI CAATTG 1 cut(s) 3087
MflI RGATCY 6 cut(s) 316, 1176, 1928, 2563, 3022, 3059
MhlI GDGCHC 6 cut(s) 234, 667, 1359, 1574, 2483, 3466
MlsI TGGCCA 4 cut(s) 539, 1684, 1775, 1964
MluI ACGCGT 1 cut(s) 710
MluNI TGGCCA 4 cut(s) 539, 1684, 1775, 1964
MlyI GAGTC 8 cut(s) 557, 1281, 1697, 1856, 1912, 2412, 2956, 3508
MmeI TCCRAC 2 cut(s) 270, 1395
Mox20I TGGCCA 4 cut(s) 539, 1684, 1775, 1964
Mph1103I ATGCAT 2 cut(s) 838, 2173
MroI TCCGGA 1 cut(s) 319
MscI TGGCCA 4 cut(s) 539, 1684, 1775, 1964
MseI TTAA 8 cut(s) 348, 1452, 2339, 2456, 2460, 3072, 3231, 3513
MslI CAYNNNNRTG 2 cut(s) 2885, 3248
Msp20I TGGCCA 4 cut(s) 539, 1684, 1775, 1964
MspA1I CMGCKG 1 cut(s) 2393
MunI CAATTG 1 cut(s) 3087
Mva1269I GAATGC 2 cut(s) 2171, 2422
MvaI CCWGG 9 cut(s) 271, 516, 596, 845, 2248, 2400, 2796, 2944, 3096
MvnI CGCG 3 cut(s) 257, 266, 712
NciI CCSGG 8 cut(s) 194, 234, 239, 338, 1122, 2603, 3341, 3394
NcoI CCATGG 3 cut(s) 485, 1312, 2742
NdeI CATATG 2 cut(s) 912, 3328
NheI GCTAGC 1 cut(s) 3155
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 4 cut(s) 1250, 1417, 1904, 2404
NruI TCGCGA 1 cut(s) 266
NsiI ATGCAT 2 cut(s) 838, 2173
NspI RCATGY 3 cut(s) 2022, 2235, 2903
NspV TTCGAA 1 cut(s) 853
PacI TTAATTAA 1 cut(s) 2460
PaeI GCATGC 1 cut(s) 2235
PaeR7I CTCGAG 3 cut(s) 107, 654, 1881
PagI TCATGA 1 cut(s) 1264
PaqCI CACCTGC 1 cut(s) 3335
PceI AGGCCT 2 cut(s) 83, 1234
PciI ACATGT 1 cut(s) 2899
PctI GAATGC 2 cut(s) 2171, 2422
PfoI TCCNGGA 3 cut(s) 514, 2942, 3094
PleI GAGTC 8 cut(s) 557, 1280, 1696, 1855, 1911, 2411, 2955, 3507
PpsI GAGTC 8 cut(s) 557, 1280, 1696, 1855, 1911, 2411, 2955, 3507
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 2 cut(s) 1718, 2792
PscI ACATGT 1 cut(s) 2899
PsiI TTATAA 1 cut(s) 2865
Psp124BI GAGCTC 1 cut(s) 3466
Psp1406I AACGTT 1 cut(s) 2298
Psp5II RGGWCCY 2 cut(s) 1718, 2792
Psp6I CCWGG 9 cut(s) 269, 514, 594, 843, 2246, 2398, 2794, 2942, 3094
PspEI GGTNACC 1 cut(s) 1250
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 9 cut(s) 269, 514, 594, 843, 2246, 2398, 2794, 2942, 3094
PspOMI GGGCCC 1 cut(s) 230
PspPPI RGGWCCY 2 cut(s) 1718, 2792
PstI CTGCAG 2 cut(s) 2911, 3146
PstNI CAGNNNCTG 4 cut(s) 1421, 1973, 2427, 2795
PsuI RGATCY 6 cut(s) 316, 1176, 1928, 2563, 3022, 3059
PvuII CAGCTG 1 cut(s) 2393
RruI TCGCGA 1 cut(s) 266
RseI CAYNNNNRTG 2 cut(s) 2885, 3248
SacI GAGCTC 1 cut(s) 3466
SaqAI TTAA 8 cut(s) 348, 1452, 2339, 2456, 2460, 3072, 3231, 3513
ScaI AGTACT 3 cut(s) 113, 2227, 3148
SchI GAGTC 8 cut(s) 557, 1281, 1697, 1856, 1912, 2412, 2956, 3508
SduI GDGCHC 6 cut(s) 234, 667, 1359, 1574, 2483, 3466
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 7 cut(s) 762, 1051, 1876, 2032, 2059, 2134, 2207
SfcI CTRYAG 4 cut(s) 27, 546, 2907, 3142
Sfr274I CTCGAG 3 cut(s) 107, 654, 1881
SfuI TTCGAA 1 cut(s) 853
SinI GGWCC 4 cut(s) 509, 1109, 1718, 2792
SlaI CTCGAG 3 cut(s) 107, 654, 1881
SmiMI CAYNNNNRTG 2 cut(s) 2885, 3248
SmlI CTYRAG 8 cut(s) 107, 128, 654, 1158, 1844, 1881, 3377, 3431
SmoI CTYRAG 8 cut(s) 107, 128, 654, 1158, 1844, 1881, 3377, 3431
SphI GCATGC 1 cut(s) 2235
SseBI AGGCCT 2 cut(s) 83, 1234
SsiI CCGC 8 cut(s) 228, 257, 260, 303, 398, 434, 1983, 3084
SstI GAGCTC 1 cut(s) 3466
StuI AGGCCT 2 cut(s) 83, 1234
StyI CCWWGG 7 cut(s) 288, 485, 540, 1312, 1474, 2107, 2742
TaaI ACNGT 9 cut(s) 1240, 1463, 1936, 2260, 2369, 2989, 3289, 3316, 3361
TaiI ACGT 5 cut(s) 39, 381, 862, 1582, 2301
TaqI TCGA 9 cut(s) 108, 655, 853, 904, 1882, 2028, 2088, 2885, 3132
TatI WGTACW 4 cut(s) 93, 111, 2225, 3146
TauI GCSGC 3 cut(s) 260, 306, 3086
Tru1I TTAA 8 cut(s) 348, 1452, 2339, 2456, 2460, 3072, 3231, 3513
Tru9I TTAA 8 cut(s) 348, 1452, 2339, 2456, 2460, 3072, 3231, 3513
TscAI CASTG 7 cut(s) 1426, 1435, 1678, 1915, 2263, 2352, 2736
TseFI GTSAC 4 cut(s) 1250, 1417, 1904, 2404
Tsp45I GTSAC 4 cut(s) 1250, 1417, 1904, 2404
TspDTI ATGAA 7 cut(s) 69, 113, 702, 1087, 1253, 1281, 1722
TspGWI ACGGA 1 cut(s) 3072
TspRI CASTG 7 cut(s) 1426, 1435, 1678, 1915, 2263, 2352, 2736
VneI GTGCAC 1 cut(s) 2479
VpaK11BI GGWCC 4 cut(s) 509, 1109, 1718, 2792
XapI RAATTY 6 cut(s) 173, 1081, 1733, 1762, 2285, 2311
XbaI TCTAGA 3 cut(s) 403, 740, 2992
XceI RCATGY 3 cut(s) 2022, 2235, 2903
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XhoI CTCGAG 3 cut(s) 107, 654, 1881
XmiI GTMKAC 2 cut(s) 1602, 3333
ZrmI AGTACT 3 cut(s) 113, 2227, 3148
Zsp2I ATGCAT 2 cut(s) 838, 2173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.