MD11G1161400.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002713
Physical Location & Seq
Forward (+)
15945007 .. 15946610
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1161400.v1.1.491

Sequence Viewer

Length: 987 bp
ATGTCTTTTTCCTTCATGTTGCAGCATTATATAGTTTACATGGGGCATCATTCGCACTCGAACTCCCAATCCGTCGTCAGAGCCAACCATGAGATTCTTGCTTCAGTCACTGGAAGAATAAACCGACTGCGCACAACACATTCCTGGGATTTTCTAGGCATAGACTCCATTCCCCAGTACAATCAAATGCCAATGGATTCAAAATCCAATGTTATTGTTGGTGTGATTGACTCTGGAGTTTGGCCGGAGTCAGAGAGCTTCAGTGATGCAGGATTAGGGCCTGTGCCAGAGAAATTCAAAGGAGAGTGTGTTACCGGTCAGAACTTTACATCTGCCAACTGCAACAGGAAAATCATAGGCTCCCGCTTCTATTTTGAAGGATTCGAAGCAGAGACTGGGCCTCTTGAGTCCAATGCACCTCTTCCCTTCTTCCGATCAGCTCGAGATAGTGATGGACATGGCACTCACACTGCCTCAACAATAGCTGGATCTAAGGTACCTAATGCCAGCTTCTTTGGGATGGCCAGAGGCACTGCCAGAGGCGGTGCGCCTAGCACCAGACTTGCTATCTACAAGGCTTGTTGGTTTGGCTTCTGTAGTGATGCAGATGTTCTTGCCGCTATGGATGATGCAATTTATGATGGTGTCGATATTCTCTCCCTTTCCCTTGGCCCTGATCCTCCGCAGCCAACTTATTTTGAAAACGCACTCTCCATTGGGGCTTTCCATGCATTCCAGAAAGGAGTACTTGTTTCTGCATCAGCTGGAAACTCTGGTTTTCCAAGTACTGCATGCAATGTTGCTCCCTGGATTCTCACAGTTGCTGCAAGCACATTGGATAGAGAATTTCATTCAAACGTATATCTGGGAAATTCAAGAATTTTAAAGGGTTCCTCACTAAACCCACTGAAAATGGAAAGATCATATGGTTTGATAGCTGCAAGTGCTGCAGCTGCCTCGGGAGTTGCAACAAAGAATGCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

329

Amino Acids

35.05

Weight (kDa)

6.55

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 69 - 278 2.3e-22 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 131
Acc65I GGTACC 1 cut(s) 496
AccB1I GGYRCC 1 cut(s) 496
AciI CCGC 4 cut(s) 364, 543, 618, 683
AclWI GGATC 2 cut(s) 496, 671
AcoI YGGCCR 2 cut(s) 242, 522
AcsI RAATTY 4 cut(s) 293, 845, 871, 879
AcuI CTGAAG 2 cut(s) 87, 244
AfaI GTAC 4 cut(s) 179, 498, 747, 787
AgeI ACCGGT 1 cut(s) 314
AgsI TTSAA 6 cut(s) 201, 298, 377, 701, 855, 876
AjnI CCWGG 2 cut(s) 143, 806
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 7 cut(s) 258, 440, 485, 510, 764, 938, 953
AluI AGCT 7 cut(s) 258, 440, 485, 510, 764, 938, 953
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 2 cut(s) 496, 671
AlwNI CAGNNNCTG 3 cut(s) 110, 395, 824
Ama87I CYCGRG 2 cut(s) 441, 958
AoxI GGCC 5 cut(s) 242, 278, 398, 522, 670
ApeKI GCWGC 7 cut(s) 22, 685, 824, 938, 947, 950, 953
ApoI RAATTY 4 cut(s) 293, 845, 871, 879
AsiGI ACCGGT 1 cut(s) 314
Asp718I GGTACC 1 cut(s) 496
AspLEI GCGC 2 cut(s) 132, 550
AspS9I GGNCC 3 cut(s) 278, 398, 671
AsuII TTCGAA 1 cut(s) 384
AvaI CYCGRG 2 cut(s) 441, 958
BalI TGGCCA 1 cut(s) 524
BanI GGYRCC 1 cut(s) 496
BbvI GCAGC 7 cut(s) 34, 697, 811, 925, 934, 940, 962
BccI CCATC 3 cut(s) 446, 514, 635
BcgI CGANNNNNNTGC 2 cut(s) 939, 973
BciT130I CCWGG 2 cut(s) 145, 808
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 2 cut(s) 155, 552
BfmI CTRYAG 2 cut(s) 595, 948
BisI GCNGC 8 cut(s) 23, 618, 686, 825, 939, 948, 951, 954
BlsI GCNGC 8 cut(s) 24, 619, 687, 826, 940, 949, 952, 955
BmcAI AGTACT 2 cut(s) 747, 787
Bme1390I CCNGG 2 cut(s) 145, 808
BmeT110I CYCGRG 2 cut(s) 441, 958
BmgT120I GGNCC 3 cut(s) 278, 398, 671
BmiI GGNNCC 3 cut(s) 361, 498, 892
BmrFI CCNGG 2 cut(s) 145, 808
BmrI ACTGGG 2 cut(s) 169, 405
BmsI GCATC 5 cut(s) 55, 256, 592, 619, 767
BmuI ACTGGG 2 cut(s) 169, 405
BpmI CTGGAG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 425
BsaJI CCNNGG 4 cut(s) 144, 667, 806, 957
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 8 cut(s) 47, 77, 294, 324, 695, 725, 735, 765
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 3 cut(s) 115, 175, 400
Bse3DI GCAATG 1 cut(s) 802
BseBI CCWGG 2 cut(s) 145, 808
BseDI CCNNGG 4 cut(s) 144, 667, 806, 957
BseGI GGATG 2 cut(s) 525, 631
BseMI GCAATG 1 cut(s) 802
BseNI ACTGG 3 cut(s) 115, 175, 400
BseXI GCAGC 7 cut(s) 34, 697, 811, 925, 934, 940, 962
BshFI GGCC 5 cut(s) 244, 280, 400, 524, 672
BshNI GGYRCC 1 cut(s) 496
BshTI ACCGGT 1 cut(s) 314
BsiHKCI CYCGRG 2 cut(s) 441, 958
BsiSI CCGG 2 cut(s) 245, 315
BsmAI GTCTC 1 cut(s) 386
BsmI GAATGC 2 cut(s) 731, 982
BsnI GGCC 5 cut(s) 244, 280, 400, 524, 672
BsoBI CYCGRG 2 cut(s) 441, 958
Bsp119I TTCGAA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 434, 488, 676, 920
BspACI CCGC 4 cut(s) 364, 543, 618, 683
BspANI GGCC 5 cut(s) 244, 280, 400, 524, 672
BspLI GGNNCC 3 cut(s) 361, 498, 892
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 2 cut(s) 496, 671
BspT104I TTCGAA 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 802
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 3 cut(s) 115, 175, 400
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 4 cut(s) 144, 667, 806, 957
BssMI GATC 4 cut(s) 434, 488, 676, 920
BssT1I CCWWGG 1 cut(s) 667
Bst2UI CCWGG 2 cut(s) 145, 808
Bst4CI ACNGT 1 cut(s) 820
Bst6I CTCTTC 1 cut(s) 426
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 508, 793, 829
BstDEI CTNAG 1 cut(s) 492
BstF5I GGATG 2 cut(s) 525, 631
BstHHI GCGC 2 cut(s) 132, 550
BstKTI GATC 4 cut(s) 437, 491, 679, 923
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 4 cut(s) 434, 488, 676, 920
BstMWI GCNNNNNNNGC 5 cut(s) 52, 728, 944, 947, 953
BstNI CCWGG 2 cut(s) 145, 808
BstNSI RCATGY 1 cut(s) 795
BstSCI CCNGG 2 cut(s) 143, 806
BstSFI CTRYAG 2 cut(s) 595, 948
BstV1I GCAGC 7 cut(s) 34, 697, 811, 925, 934, 940, 962
BstX2I RGATCY 1 cut(s) 488
BstYI RGATCY 1 cut(s) 488
BsuRI GGCC 5 cut(s) 244, 280, 400, 524, 672
BtsCI GGATG 2 cut(s) 525, 631
BtsI GCAGTG 2 cut(s) 468, 531
BtsIMutI CAGTG 5 cut(s) 108, 268, 468, 531, 905
Cac8I GCNNGC 3 cut(s) 508, 793, 829
CaiI CAGNNNCTG 3 cut(s) 110, 395, 824
CfoI GCGC 2 cut(s) 132, 550
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 278, 398, 671
Csp6I GTAC 4 cut(s) 178, 497, 746, 786
CspAI ACCGGT 1 cut(s) 314
CviAII CATG 6 cut(s) 16, 40, 89, 458, 728, 792
CviQI GTAC 4 cut(s) 178, 497, 746, 786
DdeI CTNAG 1 cut(s) 492
DpnI GATC 4 cut(s) 436, 490, 678, 922
DpnII GATC 4 cut(s) 434, 488, 676, 920
DraI TTTAAA 1 cut(s) 885
EaeI YGGCCR 2 cut(s) 242, 522
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 667
Eco57I CTGAAG 2 cut(s) 87, 244
Eco88I CYCGRG 2 cut(s) 441, 958
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 143, 806
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 6 cut(s) 19, 43, 92, 461, 731, 795
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FatI CATG 6 cut(s) 15, 39, 88, 457, 727, 791
FauI CCCGC 1 cut(s) 371
FauNDI CATATG 1 cut(s) 925
Fnu4HI GCNGC 8 cut(s) 23, 618, 686, 825, 939, 948, 951, 954
FokI GGATG 2 cut(s) 532, 638
Fsp4HI GCNGC 8 cut(s) 23, 618, 686, 825, 939, 948, 951, 954
FspBI CTAG 2 cut(s) 155, 552
FspI TGCGCA 1 cut(s) 131
GlaI GCGC 2 cut(s) 131, 549
GluI GCNGC 8 cut(s) 23, 618, 686, 825, 939, 948, 951, 954
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 5 cut(s) 244, 280, 400, 524, 672
HapII CCGG 2 cut(s) 245, 315
HhaI GCGC 2 cut(s) 132, 550
Hin1II CATG 6 cut(s) 19, 43, 92, 461, 731, 795
Hin6I GCGC 2 cut(s) 130, 548
HinP1I GCGC 2 cut(s) 130, 548
HinfI GANTC 8 cut(s) 94, 164, 197, 230, 248, 381, 407, 811
HpaII CCGG 2 cut(s) 245, 315
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 4 cut(s) 80, 253, 321, 434
Hpy188III TCNNGA 6 cut(s) 234, 404, 443, 736, 876, 960
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 22, 371, 436
HpyCH4III ACNGT 1 cut(s) 820
HpyCH4IV ACGT 1 cut(s) 858
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 728, 944, 947, 953
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 6 cut(s) 19, 43, 92, 461, 731, 795
HspAI GCGC 2 cut(s) 130, 548
KpnI GGTACC 1 cut(s) 500
Kzo9I GATC 4 cut(s) 434, 488, 676, 920
LmnI GCTCC 2 cut(s) 365, 808
Lsp1109I GCAGC 7 cut(s) 34, 697, 811, 925, 934, 940, 962
LweI GCATC 5 cut(s) 55, 256, 592, 619, 767
MaeI CTAG 2 cut(s) 155, 552
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 2 cut(s) 106, 310
MalI GATC 4 cut(s) 436, 490, 678, 922
MboI GATC 4 cut(s) 434, 488, 676, 920
MboII GAAGA 3 cut(s) 126, 413, 421
MflI RGATCY 1 cut(s) 488
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 5 cut(s) 293, 633, 845, 871, 879
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 4 cut(s) 158, 224, 257, 416
MnlI CCTC 8 cut(s) 411, 429, 484, 521, 533, 690, 904, 967
Mox20I TGGCCA 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 733
MscI TGGCCA 1 cut(s) 524
MseI TTAA 1 cut(s) 884
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 2 cut(s) 764, 953
MspI CCGG 2 cut(s) 245, 315
MspR9I CCNGG 2 cut(s) 145, 808
Mva1269I GAATGC 2 cut(s) 731, 982
MvaI CCWGG 2 cut(s) 145, 808
MwoI GCNNNNNNNGC 5 cut(s) 52, 728, 944, 947, 953
NdeI CATATG 1 cut(s) 925
NdeII GATC 4 cut(s) 434, 488, 676, 920
NlaIII CATG 6 cut(s) 19, 43, 92, 461, 731, 795
NlaIV GGNNCC 3 cut(s) 361, 498, 892
NmuCI GTSAC 1 cut(s) 106
NsbI TGCGCA 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 733
NspI RCATGY 1 cut(s) 795
NspV TTCGAA 1 cut(s) 384
PaeI GCATGC 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 441
PctI GAATGC 2 cut(s) 731, 982
PfeI GAWTC 4 cut(s) 94, 197, 381, 811
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 8 cut(s) 24, 619, 687, 826, 940, 949, 952, 955
PleI GAGTC 4 cut(s) 158, 224, 256, 415
PpsI GAGTC 4 cut(s) 158, 224, 256, 415
Psp6I CCWGG 2 cut(s) 143, 806
PspGI CCWGG 2 cut(s) 143, 806
PspN4I GGNNCC 3 cut(s) 361, 498, 892
PspPI GGNCC 3 cut(s) 278, 398, 671
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 3 cut(s) 110, 395, 824
PsuI RGATCY 1 cut(s) 488
PvuII CAGCTG 2 cut(s) 764, 953
RsaI GTAC 4 cut(s) 179, 498, 747, 787
RsaNI GTAC 4 cut(s) 178, 497, 746, 786
SaqAI TTAA 1 cut(s) 884
SatI GCNGC 8 cut(s) 23, 618, 686, 825, 939, 948, 951, 954
Sau3AI GATC 4 cut(s) 434, 488, 676, 920
Sau96I GGNCC 3 cut(s) 278, 398, 671
ScaI AGTACT 2 cut(s) 747, 787
SchI GAGTC 4 cut(s) 158, 224, 257, 416
ScrFI CCNGG 2 cut(s) 145, 808
SfaNI GCATC 5 cut(s) 55, 256, 592, 619, 767
SfcI CTRYAG 2 cut(s) 595, 948
Sfr274I CTCGAG 1 cut(s) 441
SfuI TTCGAA 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 441
SmlI CTYRAG 2 cut(s) 404, 441
SmoI CTYRAG 2 cut(s) 404, 441
SphI GCATGC 1 cut(s) 795
Sse9I AATT 5 cut(s) 293, 633, 845, 871, 879
SsiI CCGC 4 cut(s) 364, 543, 618, 683
SspMI CTAG 2 cut(s) 155, 552
StyD4I CCNGG 2 cut(s) 143, 806
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 1 cut(s) 820
TaiI ACGT 1 cut(s) 861
TaqI TCGA 4 cut(s) 59, 384, 442, 648
TasI AATT 5 cut(s) 293, 633, 845, 871, 879
TatI WGTACW 3 cut(s) 177, 745, 785
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 4 cut(s) 94, 197, 381, 811
Tru1I TTAA 1 cut(s) 884
Tru9I TTAA 1 cut(s) 884
TscAI CASTG 5 cut(s) 115, 268, 475, 538, 912
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 7 cut(s) 22, 685, 824, 938, 947, 950, 953
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 4, 839
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 5 cut(s) 115, 268, 475, 538, 912
XapI RAATTY 4 cut(s) 293, 845, 871, 879
XceI RCATGY 1 cut(s) 795
XhoI CTCGAG 1 cut(s) 441
XspI CTAG 2 cut(s) 155, 552
ZrmI AGTACT 2 cut(s) 747, 787
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.