MD11G1071500.v1.1

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001222
Physical Location & Seq
Reverse (-)
6087418 .. 6093874
6457 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1071500.v1.1.491

Sequence Viewer

Length: 3930 bp
ATGGCGGTCATGGAGCTCGAAGAGCCCAAAAAACGAAAACGCAGGACTCACAATCGACGAAAGCTTCTCGTTGGTGCCCAAGTTGAGGTGAGGAGTGTAGAAGCTGGGTTTCAGGGGTCATGGCACCCCGGGATTGTCATTGCATGCAATAATCAAGGTCATCGTCAAGTTCAATACGAACATCTTCTATGTGACGATGAGTCTGGTAATCTGGTTGATGTTGTGTCTGTTTCACCCATTTTGGATGGCATTGGTTCATTCACTGGGAACGGGAGTAACTATCGCGGGTGTATAAGGCCAACGCCACCTCACATTCAGCCAGGGGAATGGGACCTTCCCTATGGACTCTGTGTGGATGTGTTTCATGAGGACGGTTGGTGGGAAGGTGTCATCTTCGATCATGAAGATGGTTCGGAGGAGAGAAGGATCTTCTTCCCGGATTTGGGTGATGAATTAAATGCTCGGATTGACACTATCCGAATTACTCATGACTGGGATGAGGTTACTGAGAATTGGAAGCGACGCGGTACTTGGGTGTTACTTGAGTTGCTTGAGAAATATGATAACGAGAGGTACATTGCTGTTTCTGTGAAGCAAATTTGGTATGATGTGCGAGAAAAAAAGGGATTTGAGAATGTCAAGGAGTGGACTTCTCCAATGAGACATCTGTGGGAAGAGTTGGTGTTGGAGGTCATTGATGATAACATAAGCATCACAGTTGATGAACTATTAGGTGTTTTAGACAAGTCAGGATGCTTGTCACTAGAAACCCAGGTAGAATTGGAGTCAGCTCATTTGTCCTCTGATGCTAATATGAATTGTAAAGAAAATATGGTTGACTCTTCAGCTATTGTACCTGTCCACAACCCGCTGTATAGAGATCCATTAGTTGGTCATGAAGATACTTTCAAGAATGTAATGAACTGTGTACTGAACTGCAATGCTGAGTTTATTGATGAACAAGATGTAGATGGGGGTTTGTCTATTAATCCAGACTCTGCCTGCGCGCAGCAGGTTCACGAGAAATCTTATATGGCTAAATTGATCTCTGCGGCAGGGGATGACATACCAGATATGAATAGTTTGGAGTGCTCAAATATATTTTTCCAAAATAAAGAAGTGTCTGTTCTACCTCAGGTTTTGTCAGCATTTCCTTCTAATCTTGATGGGAATTCTTGTGTCAATTCTGGTATCAGAAATGGTGGAATTCGTAGTTTTAATTCCAGAAGCAAAAGTACCCTTAATTGGCTACCTTTCGACCCTCAAGCTATATATTGCCCTGACTCTGTTGACGAATATTTGAATAAGCGTTCTAATTTCAAAGTGACAGCTGTTAGGCAGCATCTTCAATATTTGGGGTGGAAAATGGAATTTGCAATCGATAAGGGCTGCTACAGATACCGGTACTTGTGTCCTGACGGAGGAGAATACGAGTACTCTCTTTTTCAAGTGTGTAAGAAGTTGAAGAAACCCAAGAGAGATACACCTTCAATCTCTGAAGATGCAACCCAGGGTTTGCATTGTTCAGAAGAACAGACTCTTCTCATTGAACAACCACAAGAGAGTCATCATCCTAGTTATTGTCCTCAGATGTCAGGGTCTTCTTACCACGAAGAATTTATTTACAAACCTGAATATTGCCACGAAGCAGTTGTGGAATATTATAACCATGCAATGGCCAAGGGACCGAAGAAGAAAGTACGAAAAATGGTATCAAAAGCAAAGAAGCACCTCTCAGCTGTTGGGTGGGTGTTCTTGTACGCAAGTAGAAAGTCAAAGAACTTGTGCTACAAATCACCCAAGGGAGTGGTGCATTTTACACTTTATAGTGCATGCAAAAGCTGTATGGATGGAGATTTTGTAGAGAGACCTGCTGAATGCATGTATGTCATTGAGGAAGACGAGGGCCAGTCCACTAGAAACAGAATATGCTCTACAGCAAGTAATTTGGTTAATCATGAAGGCTTGGTACCATCGAAAACACTGTCCAAAAATTGGTCTAGAGATGCTGTTAGCATGTCACAGGCAAGTAATCTTGTGGAGATTGGTAATGTGAAAGTTCATGGTAGTAGAAAATGCCCAAAAAAGAGAAAATATGGACCAACAGAGTTAATAGGTGATGAAAAACTAAGATGTCTAAAGGATCAATGTGCCAACCCTCCAAAGTTGAAAAGGAGAAAGGGATCTGGAGCTTTGGATGGGTTAAGAGATGAACTGGGTGGTAGTCAGCCAACTCGTGTGCTCAGGTCAAGCAAACGGGTGCAGGAAGCAACCTCCTCACACAAGAATCCTCGCAATGTCTTATCTTGGTTGATAGACAATAACATGGTTTCTCCAAGGGAAAAAGTAAATTATCGGAGCACAAAGGATAGTCATCCAATGAAACAAGGGAAGATAACTCGTGAGGGGATCAGGTGCAGCTGTTGCACAGATGTGTATACTCTTAGTTCCTTCGGGCATCACGCTGGCTGTAGTAACCACAGACCTTATGCCAATATTTTCTTGGAAGATGGTAGGTCTCTCTTAGATTGCCAGGTGCAAATTATGCATGAAAGAAGGGAGAGGAACTTCCGGAAACAACCACATGATAGGATGAAGGGCAATTGGCGCCGAGGTGAGAACGATTACATTTGCACTGTGTGTCACTTTGGTGGTGACCTAATTTTATGTGATCAATGTCCATCATCATTTCACAAGAAATGCCTTGGCTTGAAGGCTGTTCCAGACGGTGACTGGTTCTGTTCATCTTGTCGTTGTGGAATTTGTGGCCAAATGAACTTTAAAGAAGAGAAAGAGTCTATTGTGGACGATAATTGTGTTCTCACCTGTGGTCAATGCGAGCATAAATATCACAAAGGGTGCCTGAGAAAAAGAGGTGCAGAAGAAAACGAAAGTGATCCACAAGGAAACTGGTATTGCAGCAAAAGCTGTAAAAAGATATCTTTCAACCTCAACGAGCGTTTAGGAAAACAAATTCCAGTGGGTGATGACAATCTATCTTGGTCACTGTTGAAGTTCAATAAGTGTGATACCCATGCTACCGATGAACATGATACTGATGATGCCTTGACGGAGTGTTATAGCAAGCTTAATGTTGCTCTTGATGTGATGCATGAGTGTTTTGTGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGTAGAAGATGTCATCTTCAGTAGAGGGTCAGATCTTAATCGCCTGAACTTCCGAGGGTTCTATACTGTGCTTTTGGAGAGAAATGATGAATTAATCACCGCGGCTACTTTTCTTGATCAAAGGATTTTTGGAGACAAAGTGGCAGAATTACCTCTTGTTGCTACCAGATTTCAATATCGTCGGCTTGGAATGTGTCGTATATTAATGGATGAACTTGAAAAGATGCTTATGGAATTAGGTGTGGAGAGATTGGTTTTGCCCGCCGTACCAAGTGTGCTAAACACGTGGACGACATCATTTGGGTTCTCAAAGATGACACCTTCCGAGAGGCTAAAGTTTCTGGATTATACTTTCCTTGATTTTCAGGGTACCATTATGTGTCAGAAACTGTTAATGAAGAATGCTCCAGCTGAAGCAAGGCCATTAAAAAGAACCGTGCTTGATCTTTACGGTGATATCTGTGGAAGTTGTGATAATACTGACGTTGATATGTCCATTACCGTCTCTGAAGTGTATCAACCAGAACCAATTGAGGATGGCAGAATTGCGTGCCAAGGAGTAGAAAACGCTGCAGGTAATAAAAGTAGACCATGCGCGGGTGAGAGCAATCCAGAATTTTCCTCCTTCGAGGATGCTGACTGCAACAAAGTATCTCGCAAGTACTACAAGCAGTACAAGCGGAGGAGGATATCAACCAAAAGAATCCAAGTTATGCTACATGACCTCACGCTACATGATCAAAATAAATGTGGTCAGCTGTGCAGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

1310

Amino Acids

149.05

Weight (kDa)

6.4

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 25 - 103 8.5e-14 Agenet domain
DUF7028 PF22970 420 - 490 1.2e-15 Domain of unknown function (DUF7028)
DUF7028 PF22970 542 - 617 7.5e-24 Domain of unknown function (DUF7028)
TDBD PF16135 797 - 847 5.1e-21 Tify domain binding domain
PHD-1st_NSD PF23011 874 - 918 3.6e-09 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 878 - 917 2.6e-07 PHD-finger
IDM1_C PF23209 1041 - 1182 3.5e-53 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1665
Acc36I ACCTGC 3 cut(s) 1003, 1879, 3722
Acc65I GGTACC 2 cut(s) 1969, 3527
AccB1I GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3527
AccB7I CCANNNNNTGG 3 cut(s) 890, 1675, 1995
AccI GTMKAC 2 cut(s) 2438, 3745
AccII CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
AccIII TCCGGA 1 cut(s) 2571
AclWI GGATC 6 cut(s) 434, 875, 2151, 2191, 2417, 2891
AcoI YGGCCR 2 cut(s) 1677, 2767
AcsI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 2760, 2973, 3773
AcuI CTGAAG 5 cut(s) 828, 1518, 3160, 3591, 3687
AcvI CACGTG 1 cut(s) 3444
AcyI GRCGYC 1 cut(s) 2608
AdeI CACNNNGTG 1 cut(s) 2640
AfiI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3755
AflIII ACRYGT 1 cut(s) 3441
AgeI ACCGGT 1 cut(s) 1401
AhdI GACNNNNNGTC 1 cut(s) 199
AjnI CCWGG 5 cut(s) 319, 771, 1509, 2532, 3146
AleI CACNNNNGTG 2 cut(s) 2432, 2649
Alw21I GWGCWC 4 cut(s) 18, 1094, 2244, 2363
Alw26I GTCTC 5 cut(s) 655, 1861, 2523, 3285, 3667
AlwI GGATC 6 cut(s) 434, 875, 2151, 2191, 2417, 2891
AlwNI CAGNNNCTG 2 cut(s) 998, 3547
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 2571
AoxI GGCC 5 cut(s) 296, 1677, 1906, 2767, 3578
ApeKI GCWGC 6 cut(s) 1009, 1339, 1389, 2418, 2919, 3728
ApoI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 2760, 2973, 3773
ArsI GACNNNNNNTTYG 2 cut(s) 1970, 2002
AseI ATTAAT 3 cut(s) 987, 3251, 3362
AsiGI ACCGGT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 183
Asp718I GGTACC 2 cut(s) 1969, 3527
AspLEI GCGC 4 cut(s) 1007, 1009, 2610, 3755
AspS9I GGNCC 4 cut(s) 331, 1685, 1906, 2099
AsuC2I CCSGG 3 cut(s) 129, 130, 437
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 3 cut(s) 331, 1685, 2099
AxyI CCTNAGG 1 cut(s) 1134
BaeGI GKGCMC 1 cut(s) 79
BaeI ACNNNNGTAYC 4 cut(s) 519, 552, 1396, 1429
BalI TGGCCA 2 cut(s) 1679, 2769
BanI GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3527
BanII GRGCYC 2 cut(s) 18, 27
BarI GAAGNNNNNNTAC 4 cut(s) 1219, 1251, 1953, 1985
BauI CACGAG 3 cut(s) 1019, 2235, 2400
BbrPI CACGTG 1 cut(s) 3444
BbsI GAAGAC 2 cut(s) 1593, 1905
Bbv12I GWGCWC 4 cut(s) 18, 1094, 2244, 2363
BbvI GCAGC 6 cut(s) 1021, 1351, 1376, 2430, 2931, 3715
BceAI ACGGC 1 cut(s) 3407
BciT130I CCWGG 5 cut(s) 321, 773, 1511, 2534, 3148
BclI TGATCA 3 cut(s) 2671, 3274, 3895
BcnI CCSGG 3 cut(s) 129, 130, 437
BcoDI GTCTC 5 cut(s) 655, 1861, 2523, 3285, 3667
BfaI CTAG 5 cut(s) 764, 1575, 1917, 2001, 3928
BfmI CTRYAG 4 cut(s) 1393, 1935, 2470, 3729
BfoI RGCGCY 1 cut(s) 2611
BfuAI ACCTGC 3 cut(s) 1003, 1879, 3722
BglII AGATCT 2 cut(s) 3154, 3190
BisI GCNGC 8 cut(s) 1010, 1053, 1340, 1390, 2419, 2920, 3261, 3729
BlsI GCNGC 8 cut(s) 1011, 1054, 1341, 1391, 2420, 2921, 3262, 3730
BmcAI AGTACT 2 cut(s) 1436, 3821
Bme1390I CCNGG 8 cut(s) 129, 130, 321, 437, 773, 1511, 2534, 3148
Bme18I GGWCC 3 cut(s) 331, 1685, 2099
BmeRI GACNNNNNGTC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 4 cut(s) 331, 1685, 1906, 2099
BmiI GGNNCC 9 cut(s) 76, 125, 332, 1686, 1971, 2609, 2861, 3138, 3529
BmrFI CCNGG 8 cut(s) 129, 130, 321, 437, 773, 1511, 2534, 3148
BmrI ACTGGG 3 cut(s) 273, 502, 2225
BmuI ACTGGG 3 cut(s) 273, 502, 2225
BpiI GAAGAC 2 cut(s) 1593, 1905
BpmI CTGGAG 2 cut(s) 2208, 3549
Bpu10I CCTNAGC 1 cut(s) 2243
BpuEI CTTGAG 3 cut(s) 563, 572, 1248
BpuMI CCSGG 3 cut(s) 129, 130, 437
Bsa29I ATCGAT 1 cut(s) 1380
BsaAI YACGTR 1 cut(s) 3444
BsaBI GATNNNNATC 3 cut(s) 2373, 2991, 3195
BsaHI GRCGYC 1 cut(s) 2608
BsaI GGTCTC 2 cut(s) 1861, 2523
BsaWI WCCGGW 2 cut(s) 1401, 2571
BsaXI ACNNNNNCTCC 4 cut(s) 1845, 1875, 3282, 3312
Bsc4I CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3755
Bse118I RCCGGY 1 cut(s) 1401
Bse1I ACTGG 7 cut(s) 268, 497, 1909, 2220, 2738, 2915, 2978
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
Bse8I GATNNNNATC 3 cut(s) 2373, 2991, 3195
BseAI TCCGGA 1 cut(s) 2571
BseBI CCWGG 5 cut(s) 321, 773, 1511, 2534, 3148
BseCI ATCGAT 1 cut(s) 1380
BseJI GATNNNNATC 3 cut(s) 2373, 2991, 3195
BseLI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3755
BseMI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
BseMII CTCAG 7 cut(s) 498, 936, 1148, 1601, 1749, 2257, 2855
BseNI ACTGG 7 cut(s) 268, 497, 1909, 2220, 2738, 2915, 2978
BsePI GCGCGC 1 cut(s) 1005
BseRI GAGGAG 5 cut(s) 106, 431, 1437, 2266, 3856
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 6 cut(s) 1021, 1351, 1376, 2430, 2931, 3715
BseYI CCCAGC 1 cut(s) 104
BsgI GTGCAG 3 cut(s) 2282, 2437, 2898
Bsh1236I CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
BshFI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3580
BshNI GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3527
BshTI ACCGGT 1 cut(s) 1401
BshVI ATCGAT 1 cut(s) 1380
BsiHKAI GWGCWC 4 cut(s) 18, 1094, 2244, 2363
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 4 cut(s) 129, 437, 1402, 2572
BslFI GGGAC 2 cut(s) 344, 1698
BslI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3755
BsmAI GTCTC 5 cut(s) 655, 1861, 2523, 3285, 3667
BsmBI CGTCTC 1 cut(s) 3667
BsmFI GGGAC 2 cut(s) 344, 1698
BsmI GAATGC 2 cut(s) 1883, 3565
BsnI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3580
Bso31I GGTCTC 2 cut(s) 1861, 2523
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
Bsp13I TCCGGA 1 cut(s) 2571
BspANI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3580
BspCNI CTCAG 7 cut(s) 499, 937, 1147, 1600, 1748, 2256, 2856
BspDI ATCGAT 1 cut(s) 1380
BspEI TCCGGA 1 cut(s) 2571
BspFNI CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
BspHI TCATGA 5 cut(s) 364, 400, 487, 895, 1957
BspLI GGNNCC 9 cut(s) 76, 125, 332, 1686, 1971, 2609, 2861, 3138, 3529
BspMAI CTGCAG 1 cut(s) 3733
BspMI ACCTGC 3 cut(s) 1003, 1879, 3722
BspPI GGATC 6 cut(s) 434, 875, 2151, 2191, 2417, 2891
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3527
BspTNI GGTCTC 2 cut(s) 1861, 2523
BsrDI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
BsrFI RCCGGY 1 cut(s) 1401
BsrI ACTGG 7 cut(s) 268, 497, 1909, 2220, 2738, 2915, 2978
BssAI RCCGGY 1 cut(s) 1401
BssHII GCGCGC 1 cut(s) 1005
BssNAI GTATAC 1 cut(s) 2439
BssNI GRCGYC 1 cut(s) 2608
BssSI CACGAG 3 cut(s) 1019, 2235, 2400
BssT1I CCWWGG 5 cut(s) 1680, 1800, 2336, 2704, 3712
Bst1107I GTATAC 1 cut(s) 2439
Bst2BI CACGAG 3 cut(s) 1019, 2235, 2400
Bst2UI CCWGG 5 cut(s) 321, 773, 1511, 2534, 3148
Bst6I CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
BstACI GRCGYC 1 cut(s) 2608
BstAPI GCANNNNNTGC 2 cut(s) 2424, 2545
BstBAI YACGTR 1 cut(s) 3444
BstC8I GCNNGC 9 cut(s) 145, 1003, 1007, 1834, 2467, 2840, 3086, 3420, 3709
BstDSI CCRYGG 1 cut(s) 3258
BstEII GGTNACC 1 cut(s) 2654
BstENI CCTNNNNNAGG 1 cut(s) 1419
BstFNI CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
BstH2I RGCGCY 1 cut(s) 2611
BstHHI GCGC 4 cut(s) 1007, 1009, 2610, 3755
BstMAI GTCTC 5 cut(s) 655, 1861, 2523, 3285, 3667
BstMWI GCNNNNNNNGC 6 cut(s) 22, 2424, 2545, 2607, 2925, 3835
BstNI CCWGG 5 cut(s) 321, 773, 1511, 2534, 3148
BstNSI RCATGY 4 cut(s) 147, 1836, 1885, 2020
BstPI GGTNACC 1 cut(s) 2654
BstSCI CCNGG 8 cut(s) 127, 128, 319, 435, 771, 1509, 2532, 3146
BstSFI CTRYAG 4 cut(s) 1393, 1935, 2470, 3729
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
BstV1I GCAGC 6 cut(s) 1021, 1351, 1376, 2430, 2931, 3715
BstV2I GAAGAC 2 cut(s) 1593, 1905
BstX2I RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
BstXI CCANNNNNNTGG 2 cut(s) 327, 1807
BstYI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
BstZ17I GTATAC 1 cut(s) 2439
Bsu15I ATCGAT 1 cut(s) 1380
Bsu36I CCTNAGG 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3580
BsuTUI ATCGAT 1 cut(s) 1380
BtgI CCRYGG 1 cut(s) 3258
BtsI GCAGTG 1 cut(s) 3928
BtsIMutI CAGTG 6 cut(s) 261, 1982, 2634, 2985, 3005, 3928
BveI ACCTGC 3 cut(s) 1003, 1879, 3722
Cac8I GCNNGC 9 cut(s) 145, 1003, 1007, 1834, 2467, 2840, 3086, 3420, 3709
CaiI CAGNNNCTG 2 cut(s) 998, 3547
CciI TCATGA 5 cut(s) 364, 400, 487, 895, 1957
CfoI GCGC 4 cut(s) 1007, 1009, 2610, 3755
Cfr10I RCCGGY 1 cut(s) 1401
Cfr13I GGNCC 4 cut(s) 331, 1685, 1906, 2099
Cfr42I CCGCGG 1 cut(s) 3261
Cfr9I CCCGGG 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 1380
CseI GACGC 1 cut(s) 531
CspAI ACCGGT 1 cut(s) 1401
DinI GGCGCC 1 cut(s) 2609
DraI TTTAAA 1 cut(s) 2782
DraIII CACNNNGTG 1 cut(s) 2640
DriI GACNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 2 cut(s) 1677, 2767
Eam1104I CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 5 cut(s) 1680, 1800, 2336, 2704, 3712
Eco24I GRGCYC 2 cut(s) 18, 27
Eco31I GGTCTC 2 cut(s) 1861, 2523
Eco32I GATATC 3 cut(s) 2940, 3616, 3849
Eco47I GGWCC 3 cut(s) 331, 1685, 2099
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 5 cut(s) 828, 1518, 3160, 3591, 3687
Eco72I CACGTG 1 cut(s) 3444
Eco81I CCTNAGG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 128
Eco91I GGTNACC 1 cut(s) 2654
EcoICRI GAGCTC 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 1419
EcoO109I RGGNCCY 1 cut(s) 331
EcoO65I GGTNACC 1 cut(s) 2654
EcoRI GAATTC 2 cut(s) 1171, 1206
EcoRII CCWGG 5 cut(s) 319, 771, 1509, 2532, 3146
EcoRV GATATC 3 cut(s) 2940, 3616, 3849
EcoT14I CCWWGG 5 cut(s) 1680, 1800, 2336, 2704, 3712
EcoT22I ATGCAT 3 cut(s) 1883, 2550, 3114
EcoT38I GRGCYC 2 cut(s) 18, 27
EgeI GGCGCC 1 cut(s) 2609
EheI GGCGCC 1 cut(s) 2609
ErhI CCWWGG 5 cut(s) 1680, 1800, 2336, 2704, 3712
Esp3I CGTCTC 1 cut(s) 3667
FaqI GGGAC 2 cut(s) 344, 1698
FauI CCCGC 4 cut(s) 278, 876, 3427, 3748
FbaI TGATCA 3 cut(s) 2671, 3274, 3895
FblI GTMKAC 2 cut(s) 2438, 3745
Fnu4HI GCNGC 8 cut(s) 1010, 1053, 1340, 1390, 2419, 2920, 3261, 3729
FriOI GRGCYC 2 cut(s) 18, 27
Fsp4HI GCNGC 8 cut(s) 1010, 1053, 1340, 1390, 2419, 2920, 3261, 3729
FspBI CTAG 5 cut(s) 764, 1575, 1917, 2001, 3928
GlaI GCGC 4 cut(s) 1006, 1008, 2609, 3754
GluI GCNGC 8 cut(s) 1010, 1053, 1340, 1390, 2419, 2920, 3261, 3729
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 2 cut(s) 2208, 3549
HaeII RGCGCY 1 cut(s) 2611
HaeIII GGCC 5 cut(s) 298, 1679, 1908, 2769, 3580
HapII CCGG 4 cut(s) 129, 437, 1402, 2572
HgaI GACGC 1 cut(s) 531
HhaI GCGC 4 cut(s) 1007, 1009, 2610, 3755
Hin1I GRCGYC 1 cut(s) 2608
Hin6I GCGC 4 cut(s) 1005, 1007, 2608, 3753
HinP1I GCGC 4 cut(s) 1005, 1007, 2608, 3753
HincII GTYRAC 2 cut(s) 838, 1291
HindII GTYRAC 2 cut(s) 838, 1291
HindIII AAGCTT 2 cut(s) 62, 3086
HpaII CCGG 4 cut(s) 129, 437, 1402, 2572
Hpy99I CGWCG 3 cut(s) 60, 525, 3342
HpyCH4IV ACGT 2 cut(s) 3443, 3642
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 2424, 2545, 2607, 2925, 3835
HpySE526I ACGT 2 cut(s) 3443, 3642
Hsp92I GRCGYC 1 cut(s) 2608
HspAI GCGC 4 cut(s) 1005, 1007, 2608, 3753
KasI GGCGCC 1 cut(s) 2607
Kpn2I TCCGGA 1 cut(s) 2571
KpnI GGTACC 2 cut(s) 1973, 3531
Ksp22I TGATCA 3 cut(s) 2671, 3274, 3895
KspI CCGCGG 1 cut(s) 3261
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 4 cut(s) 13, 2189, 2358, 3568
Lsp1109I GCAGC 6 cut(s) 1021, 1351, 1376, 2430, 2931, 3715
MaeI CTAG 5 cut(s) 764, 1575, 1917, 2001, 3928
MaeII ACGT 2 cut(s) 3443, 3642
MfeI CAATTG 2 cut(s) 2602, 3687
MflI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
MhlI GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
MlsI TGGCCA 2 cut(s) 1679, 2769
MluNI TGGCCA 2 cut(s) 1679, 2769
Mly113I GGCGCC 1 cut(s) 2608
MmeI TCCRAC 1 cut(s) 666
Mox20I TGGCCA 2 cut(s) 1679, 2769
Mph1103I ATGCAT 3 cut(s) 1883, 2550, 3114
MroI TCCGGA 1 cut(s) 2571
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 2 cut(s) 1679, 2769
MslI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
Msp20I TGGCCA 2 cut(s) 1679, 2769
MspA1I CMGCKG 7 cut(s) 871, 1331, 1739, 2421, 3260, 3569, 3916
MspI CCGG 4 cut(s) 129, 437, 1402, 2572
MspR9I CCNGG 8 cut(s) 129, 130, 321, 437, 773, 1511, 2534, 3148
MunI CAATTG 2 cut(s) 2602, 3687
Mva1269I GAATGC 2 cut(s) 1883, 3565
MvaI CCWGG 5 cut(s) 321, 773, 1511, 2534, 3148
MvnI CGCG 5 cut(s) 285, 525, 1007, 3260, 3755
MwoI GCNNNNNNNGC 6 cut(s) 22, 2424, 2545, 2607, 2925, 3835
NarI GGCGCC 1 cut(s) 2608
NciI CCSGG 3 cut(s) 129, 130, 437
NlaIV GGNNCC 9 cut(s) 76, 125, 332, 1686, 1971, 2609, 2861, 3138, 3529
NmeAIII GCCGAG 1 cut(s) 2636
NmuCI GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
NsiI ATGCAT 3 cut(s) 1883, 2550, 3114
NspI RCATGY 4 cut(s) 147, 1836, 1885, 2020
OliI CACNNNNGTG 2 cut(s) 2432, 2649
PaeI GCATGC 2 cut(s) 147, 1836
PagI TCATGA 5 cut(s) 364, 400, 487, 895, 1957
PasI CCCWGGG 1 cut(s) 1510
PauI GCGCGC 1 cut(s) 1005
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 2 cut(s) 1883, 3565
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 2287, 3861
PflMI CCANNNNNTGG 3 cut(s) 890, 1675, 1995
PfoI TCCNGGA 1 cut(s) 435
PinAI ACCGGT 1 cut(s) 1401
PkrI GCNGC 8 cut(s) 1011, 1054, 1341, 1391, 2420, 2921, 3262, 3730
PluTI GGCGCC 1 cut(s) 2611
PmaCI CACGTG 1 cut(s) 3444
PmlI CACGTG 1 cut(s) 3444
Ppu21I YACGTR 1 cut(s) 3444
PpuMI RGGWCCY 1 cut(s) 331
PshBI ATTAAT 3 cut(s) 987, 3251, 3362
PsiI TTATAA 1 cut(s) 1665
Psp124BI GAGCTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 5 cut(s) 319, 771, 1509, 2532, 3146
PspCI CACGTG 1 cut(s) 3444
PspEI GGTNACC 1 cut(s) 2654
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 5 cut(s) 319, 771, 1509, 2532, 3146
PspN4I GGNNCC 9 cut(s) 76, 125, 332, 1686, 1971, 2609, 2861, 3138, 3529
PspPI GGNCC 4 cut(s) 331, 1685, 1906, 2099
PspPPI RGGWCCY 1 cut(s) 331
PsrI GAACNNNNNNTAC 2 cut(s) 1772, 1804
PstI CTGCAG 1 cut(s) 3733
PstNI CAGNNNCTG 2 cut(s) 998, 3547
PsuI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
PteI GCGCGC 1 cut(s) 1005
PvuII CAGCTG 5 cut(s) 1331, 1739, 2421, 3569, 3916
RseI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
SacI GAGCTC 1 cut(s) 18
SacII CCGCGG 1 cut(s) 3261
SapI GCTCTTC 1 cut(s) 15
SatI GCNGC 8 cut(s) 1010, 1053, 1340, 1390, 2419, 2920, 3261, 3729
Sau96I GGNCC 4 cut(s) 331, 1685, 1906, 2099
ScaI AGTACT 2 cut(s) 1436, 3821
ScrFI CCNGG 8 cut(s) 129, 130, 321, 437, 773, 1511, 2534, 3148
SduI GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
SfcI CTRYAG 4 cut(s) 1393, 1935, 2470, 3729
SfoI GGCGCC 1 cut(s) 2609
Sfr303I CCGCGG 1 cut(s) 3261
SgrBI CCGCGG 1 cut(s) 3261
SinI GGWCC 3 cut(s) 331, 1685, 2099
SmaI CCCGGG 1 cut(s) 130
SmiMI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
SmlI CTYRAG 3 cut(s) 542, 551, 1263
SmoI CTYRAG 3 cut(s) 542, 551, 1263
SphI GCATGC 2 cut(s) 147, 1836
SspDI GGCGCC 1 cut(s) 2607
SspI AATATT 5 cut(s) 1298, 1352, 1637, 1661, 2497
SspMI CTAG 5 cut(s) 764, 1575, 1917, 2001, 3928
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 8 cut(s) 127, 128, 319, 435, 771, 1509, 2532, 3146
StyI CCWWGG 5 cut(s) 1680, 1800, 2336, 2704, 3712
TaiI ACGT 2 cut(s) 3446, 3645
TaqI TCGA 7 cut(s) 18, 55, 396, 1257, 1380, 1976, 3786
TaqII GACCGA 1 cut(s) 1702
TatI WGTACW 4 cut(s) 928, 1434, 3819, 3831
TauI GCSGC 2 cut(s) 1055, 3263
TfiI GAWTC 2 cut(s) 2287, 3861
TscAI CASTG 6 cut(s) 268, 1989, 2641, 2985, 3012, 3928
TseFI GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
TseI GCWGC 6 cut(s) 1009, 1339, 1389, 2418, 2919, 3728
Tsp45I GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
TspGWI ACGGA 2 cut(s) 1434, 3086
TspMI CCCGGG 1 cut(s) 128
TspRI CASTG 6 cut(s) 268, 1989, 2641, 2985, 3012, 3928
Van91I CCANNNNNTGG 3 cut(s) 890, 1675, 1995
VpaK11BI GGWCC 3 cut(s) 331, 1685, 2099
VspI ATTAAT 3 cut(s) 987, 3251, 3362
XagI CCTNNNNNAGG 1 cut(s) 1419
XapI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 2760, 2973, 3773
XbaI TCTAGA 1 cut(s) 2000
XceI RCATGY 4 cut(s) 147, 1836, 1885, 2020
XcmI CCANNNNNNNNNTGG 3 cut(s) 2500, 2730, 2907
XmaI CCCGGG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 2438, 3745
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 5 cut(s) 764, 1575, 1917, 2001, 3928
ZrmI AGTACT 2 cut(s) 1436, 3821
Zsp2I ATGCAT 3 cut(s) 1883, 2550, 3114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.