Rroxscaffold_7G00212520

ubiquitinyl hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001222
Physical Location & Seq
Forward (+)
63004810 .. 63009613
4804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212520.1

Sequence Viewer

Length: 2340 bp
ATGTACGCAAAAAAGTGGACCAGAAATCGTGATATTATGTCATGGTGGAGCAATGTTGTGGAGTTTGACAATGTGAATAGCGAAAACGAACACAATGCTAACCCTCCAAAGCTGAAAAGGCGAAAGGCATCTGCATCTTCTTCTGGATACAGAACTGGTCTGGTTGGTAGTAAGCCAACTCGTGTGATGAGGTCACGAAAACAAGTGCAGGAAAAGGTTACTCTTACTTCCACACACTATCATCCTTGTACAATCTTATCATGGCTGATTGACAATGATGTGGTTTTTCCAAGTGAAAAAGTACATTATTGTACTGCAAAGGGCAGTCATCCAATGGCACAAGGGACAATAAGTCGAGTGGGGATAGTATGCAACTGTTGCCAGAAGGTGTTTAGTCTTTGTGACTTTGAAAGTCATGCAGGCTGCAGTGAGAATCATGTTGGCTGCAGTAACCACAGCCCTGCAGCCAATATTTTTCTGGACGATGGAAGGTCTATCTTAGATTGCCAGATACAACTCTTAAGTGAAAAAAAGGAAGAGGAGCTGCAGATCACAACCACATGGATGTTCCAGATGGTGACTGGTTCTGTCCAGCTTGCTGTTGTGGACTTTGTGGCCAAAGCATACTCACGAAAGACAGAAAACCTACCATGCATTATCGTACCTGTGGTCAATGCGAGCGTGAATGTAGATCACACTGGGTGCCTGAGGAAAATAAGTGCAGATATGTTGGAAAGTGATCCAAAAGGGAACTGGTTTTGCAAAGAGAGAGCTGTAAAAAGGTATCTCCATCACCCTTTGGAGCTCAGTGTTATATACTTAGGCCTCCACGAGTGTTTAGGGAAACAATTTCCAGCGGGTATTGACAGTCTAACCTGGTCACTATTGAAGTCTGACAGTAGAGATACTAATGAGCCTAATAATGATGCCATCACGGAGACTTACAGCAGGCTCAATCTAGCCGTTATTGTGATGCACGAGTGCTTTAAGCCTTTTAAGAAACCTCATACTGGTACAGATCTTGCAGAAGATATCATATTCAGTAGAGGGTCGGATCGTAATCGCATGAACTGTCAAGGGTTTTATACAATGCTTTTGGAGAAAAATGATGAAATAATAACTGTAGCTACTGTAAGGATCTATGGAGCACAGGTGGCAGAAATACCACTTATTGGTACCAAATTCCAATATCGTCGAGGTGGAATGTGTCGTTTATTAATGCATCTGCTTGAAAAGATGCTAATGAATTTAGGTGTGGAGAGACTGGTCTTGCCCACTGTCCCCTGTTTACTAGACAATTGGTCATCATTTGGATTTTCAAAGATGACGGATTCTCAGAGGCTACAGGTTCTAGGAACTCCACTTGTTATCTCTGGAAGTGGTGATAATGTCTCAATCACATGGAAAATTGACAATTTCTCTACTTTGCTGTGTATCAAGAAGTACTCTGACACTTTCCTCACTGGTCAATTGAGATGGCAGATTCTTTTGTATCCAAAGGGAAGGGAATCTGTAGACGACTTGTCGGTGTCTTTTGATGTTGAAGGTGGCTCAGAATTGCCACTTGGGTGGTATAGATATGCACAACTCAGCATGACCATGGTTAATCAACTTCAGCGCAGCAAGTCAATAAGCAAAGAAAGTAAACATGTTTTCAATAAAGGGGGAGTGACTCTGTCTGATGCCAGTTCTGAGCCTATGGAAGTTCCAGATCTTTCTTCAGCTACACGGAAGGCAACTAGCTTTCAACATATGATTGCCTCCCAGGACACAGCAAGTTCTGCAGAAGTTATCAAGTTTTTGTCTGATACCAGTACTGACCATTCCCTCGATGAGGAATTTGATGTTGTGTCCTCCACATCACCTTCTGATCTCTTGAACTTCAGGGGCTTGGCGGATATAAAGAAAGATTTTGTTCCCCTACTCGAGGAAGTTTGTTTATGGCATCCTTCTCTTATTGAATGCCAAAGGGCGAGTAGTCCCCAGTTCACTGAATGGGCATTTACTGCCTTGGGAAAAGTCCTGCATTTTTTGAAGACCAAAAAGGTTAAGGAAATGACAACATATGTTTGTAAAGACCTACAAATTTTATGGAATGAGCTGAAGGCCTTCGGATTTGACCTGACATGGCTAAAGCCTCATGTTGAAAGAGCTCTCGGTATGAGTAAGTTTGTGGAAAGCACAAAGGAGCTAAAAAGAGTAGCAGAGAATGTAAAGGCTTTGGAGATTGAATCAAAGAGGCTAGAGGCTAAGCTTGTGGCAGCGAGAAGTGATCTGGAGAAGGCTGGACAAGGCTTCGAAGAGATTATGGATAGTGAGTTAGGCTATCATACTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

779

Amino Acids

87.76

Weight (kDa)

6.89

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 115 - 172 2.1e-18 Tify domain binding domain
IDM1_C PF23209 329 - 455 1.4e-43 Increased DNA methylation 1, C-terminal domain
MATH_2 PF22486 466 - 554 1.6e-15 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1175
AccB1I GGYRCC 2 cut(s) 702, 1175
AccB7I CCANNNNNTGG 1 cut(s) 1172
AccI GTMKAC 1 cut(s) 1515
AciI CCGC 2 cut(s) 857, 1895
AclWI GGATC 3 cut(s) 734, 1062, 1145
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 4 cut(s) 1181, 1246, 1838, 2085
AcuI CTGAAG 4 cut(s) 1598, 1704, 1867, 2123
AdeI CACNNNGTG 1 cut(s) 702
AfaI GTAC 9 cut(s) 5, 250, 303, 313, 663, 1015, 1177, 1445, 1816
AfiI CCNNNNNNNGG 4 cut(s) 1010, 1172, 1834, 1927
AflII CTTAAG 1 cut(s) 520
AflIII ACRYGT 1 cut(s) 1648
AhdI GACNNNNNGTC 3 cut(s) 351, 1300, 1522
AjnI CCWGG 2 cut(s) 875, 1764
AjuI GAANNNNNNNTTGG 2 cut(s) 1548, 1580
AleI CACNNNNGTG 1 cut(s) 1567
Alw21I GWGCWC 3 cut(s) 807, 1150, 2155
Alw26I GTCTC 3 cut(s) 932, 1255, 1396
AlwI GGATC 3 cut(s) 734, 1062, 1145
Ama87I CYCGRG 1 cut(s) 1925
AoxI GGCC 3 cut(s) 615, 823, 2106
ApeKI GCWGC 6 cut(s) 423, 444, 464, 544, 1620, 2261
ApoI RAATTY 4 cut(s) 1181, 1246, 1838, 2085
AseI ATTAAT 1 cut(s) 1217
Asp700I GAANNNNTTC 1 cut(s) 2108
Asp718I GGTACC 1 cut(s) 1175
AspLEI GCGC 1 cut(s) 1620
AspS9I GGNCC 1 cut(s) 18
AsuHPI GGTGA 4 cut(s) 589, 785, 1394, 1854
AsuII TTCGAA 1 cut(s) 2298
AvaI CYCGRG 1 cut(s) 1925
AvaII GGWCC 1 cut(s) 18
AxyI CCTNAGG 1 cut(s) 707
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 2 cut(s) 702, 1175
BanII GRGCYC 2 cut(s) 807, 2155
BauI CACGAG 3 cut(s) 180, 830, 977
BbsI GAAGAC 1 cut(s) 2042
Bbv12I GWGCWC 3 cut(s) 807, 1150, 2155
BbvI GCAGC 6 cut(s) 410, 431, 476, 531, 1632, 2273
BccI CCATC 5 cut(s) 479, 568, 798, 938, 1470
BceAI ACGGC 1 cut(s) 947
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 2 cut(s) 877, 1766
BciVI GTATCC 2 cut(s) 140, 1503
BcoDI GTCTC 3 cut(s) 932, 1255, 1396
BfaI CTAG 5 cut(s) 959, 1292, 1352, 1740, 2243
BfmI CTRYAG 8 cut(s) 424, 445, 462, 545, 1122, 1343, 1512, 1782
BfrI CTTAAG 1 cut(s) 520
BfuI GTATCC 2 cut(s) 140, 1503
BglII AGATCT 2 cut(s) 1018, 1711
BisI GCNGC 6 cut(s) 424, 445, 465, 545, 1621, 2262
BlpI GCTNAGC 1 cut(s) 2250
BlsI GCNGC 6 cut(s) 425, 446, 466, 546, 1622, 2263
BmcAI AGTACT 2 cut(s) 1445, 1816
Bme1390I CCNGG 2 cut(s) 877, 1766
Bme18I GGWCC 1 cut(s) 18
BmeRI GACNNNNNGTC 3 cut(s) 351, 1300, 1522
BmeT110I CYCGRG 1 cut(s) 1925
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 704, 1177
BmrFI CCNGG 2 cut(s) 877, 1766
BmrI ACTGGG 2 cut(s) 708, 1978
BmsI GCATC 8 cut(s) 137, 143, 916, 963, 1227, 1231, 1672, 1954
BmuI ACTGGG 2 cut(s) 708, 1978
BpiI GAAGAC 1 cut(s) 2042
BpmI CTGGAG 1 cut(s) 2297
Bpu1102I GCTNAGC 1 cut(s) 2250
Bpu14I TTCGAA 1 cut(s) 2298
BsaBI GATNNNNATC 1 cut(s) 1059
BsaJI CCNNGG 3 cut(s) 1599, 1764, 2010
BsaXI ACNNNNNCTCC 2 cut(s) 1137, 1167
Bsc4I CCNNNNNNNGG 4 cut(s) 1010, 1172, 1834, 1927
Bse21I CCTNAGG 1 cut(s) 707
Bse3DI GCAATG 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 877, 1766
BseDI CCNNGG 3 cut(s) 1599, 1764, 2010
BseGI GGATG 4 cut(s) 241, 328, 570, 1945
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 4 cut(s) 1010, 1172, 1834, 1927
BseMI GCAATG 1 cut(s) 58
BseMII CTCAG 6 cut(s) 698, 820, 1349, 1566, 1603, 1683
BseRI GAGGAG 1 cut(s) 554
BseXI GCAGC 6 cut(s) 410, 431, 476, 531, 1632, 2273
BsgI GTGCAG 2 cut(s) 227, 741
BshFI GGCC 3 cut(s) 617, 825, 2108
BshNI GGYRCC 2 cut(s) 702, 1175
BsiHKAI GWGCWC 3 cut(s) 807, 1150, 2155
BsiHKCI CYCGRG 1 cut(s) 1925
BslFI GGGAC 3 cut(s) 358, 1265, 1965
BslI CCNNNNNNNGG 4 cut(s) 1010, 1172, 1834, 1927
BsmAI GTCTC 3 cut(s) 932, 1255, 1396
BsmFI GGGAC 3 cut(s) 358, 1265, 1965
BsmI GAATGC 1 cut(s) 1967
BsnI GGCC 3 cut(s) 617, 825, 2108
BsoBI CYCGRG 1 cut(s) 1925
Bsp119I TTCGAA 1 cut(s) 2298
Bsp1286I GDGCHC 3 cut(s) 807, 1150, 2155
Bsp1407I TGTACA 1 cut(s) 248
Bsp143I GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
Bsp1720I GCTNAGC 1 cut(s) 2250
Bsp19I CCATGG 1 cut(s) 1599
BspACI CCGC 2 cut(s) 857, 1895
BspANI GGCC 3 cut(s) 617, 825, 2108
BspCNI CTCAG 6 cut(s) 699, 819, 1348, 1565, 1602, 1684
BspLI GGNNCC 2 cut(s) 704, 1177
BspMAI CTGCAG 5 cut(s) 428, 449, 466, 549, 1786
BspPI GGATC 3 cut(s) 734, 1062, 1145
BspT104I TTCGAA 1 cut(s) 2298
BspT107I GGYRCC 2 cut(s) 702, 1175
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 1599, 1764, 2010
BssMI GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
BssSI CACGAG 3 cut(s) 180, 830, 977
BssT1I CCWWGG 2 cut(s) 1599, 2010
Bst2BI CACGAG 3 cut(s) 180, 830, 977
Bst2UI CCWGG 2 cut(s) 877, 1766
Bst4CI ACNGT 7 cut(s) 377, 869, 899, 1073, 1123, 1132, 1279
Bst6I CTCTTC 2 cut(s) 531, 2295
BstAFI CTTAAG 1 cut(s) 520
BstAPI GCANNNNNTGC 3 cut(s) 378, 1781, 2006
BstAUI TGTACA 1 cut(s) 248
BstBI TTCGAA 1 cut(s) 2298
BstC8I GCNNGC 4 cut(s) 421, 597, 679, 950
BstDEI CTNAG 9 cut(s) 499, 707, 806, 820, 1335, 1552, 1589, 1692, 2250
BstDSI CCRYGG 1 cut(s) 1599
BstENI CCTNNNNNAGG 2 cut(s) 1832, 1925
BstF5I GGATG 4 cut(s) 241, 328, 570, 1945
BstHHI GCGC 1 cut(s) 1620
BstKTI GATC 9 cut(s) 552, 694, 742, 1021, 1057, 1140, 1714, 1873, 2275
BstMAI GTCTC 3 cut(s) 932, 1255, 1396
BstMBI GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
BstMWI GCNNNNNNNGC 5 cut(s) 118, 378, 1154, 1781, 2006
BstNI CCWGG 2 cut(s) 877, 1766
BstNSI RCATGY 1 cut(s) 1652
BstSCI CCNGG 2 cut(s) 875, 1764
BstSFI CTRYAG 8 cut(s) 424, 445, 462, 545, 1122, 1343, 1512, 1782
BstV1I GCAGC 6 cut(s) 410, 431, 476, 531, 1632, 2273
BstV2I GAAGAC 1 cut(s) 2042
BstX2I RGATCY 3 cut(s) 1018, 1137, 1711
BstXI CCANNNNNNTGG 1 cut(s) 1569
BstYI RGATCY 3 cut(s) 1018, 1137, 1711
Bsu36I CCTNAGG 1 cut(s) 707
BsuI GTATCC 2 cut(s) 140, 1503
BsuRI GGCC 3 cut(s) 617, 825, 2108
BtgI CCRYGG 1 cut(s) 1599
BtsCI GGATG 4 cut(s) 241, 328, 570, 1945
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 6 cut(s) 433, 696, 814, 1275, 1461, 1989
Cac8I GCNNGC 4 cut(s) 421, 597, 679, 950
CfoI GCGC 1 cut(s) 1620
Cfr13I GGNCC 1 cut(s) 18
CsiI ACCWGGT 1 cut(s) 875
Csp6I GTAC 9 cut(s) 4, 249, 302, 312, 662, 1014, 1176, 1444, 1815
CviQI GTAC 9 cut(s) 4, 249, 302, 312, 662, 1014, 1176, 1444, 1815
DdeI CTNAG 9 cut(s) 499, 707, 806, 820, 1335, 1552, 1589, 1692, 2250
DpnI GATC 9 cut(s) 551, 693, 741, 1020, 1056, 1139, 1713, 1872, 2274
DpnII GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
DraIII CACNNNGTG 1 cut(s) 702
DriI GACNNNNNGTC 3 cut(s) 351, 1300, 1522
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 531, 2295
Eam1105I GACNNNNNGTC 3 cut(s) 351, 1300, 1522
EarI CTCTTC 2 cut(s) 531, 2295
EciI GGCGGA 1 cut(s) 1910
Ecl136II GAGCTC 2 cut(s) 805, 2153
Eco130I CCWWGG 2 cut(s) 1599, 2010
Eco147I AGGCCT 2 cut(s) 825, 2108
Eco24I GRGCYC 2 cut(s) 807, 2155
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 1 cut(s) 18
Eco53kI GAGCTC 2 cut(s) 805, 2153
Eco57I CTGAAG 4 cut(s) 1598, 1704, 1867, 2123
Eco81I CCTNAGG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 1925
EcoICRI GAGCTC 2 cut(s) 805, 2153
EcoNI CCTNNNNNAGG 2 cut(s) 1832, 1925
EcoRII CCWGG 2 cut(s) 875, 1764
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 2 cut(s) 1599, 2010
EcoT22I ATGCAT 2 cut(s) 656, 1224
EcoT38I GRGCYC 2 cut(s) 807, 2155
ErhI CCWWGG 2 cut(s) 1599, 2010
FaqI GGGAC 3 cut(s) 358, 1265, 1965
FauI CCCGC 1 cut(s) 850
FauNDI CATATG 2 cut(s) 1752, 2065
FblI GTMKAC 1 cut(s) 1515
Fnu4HI GCNGC 6 cut(s) 424, 445, 465, 545, 1621, 2262
FokI GGATG 4 cut(s) 228, 315, 577, 1932
FriOI GRGCYC 2 cut(s) 807, 2155
Fsp4HI GCNGC 6 cut(s) 424, 445, 465, 545, 1621, 2262
FspBI CTAG 5 cut(s) 959, 1292, 1352, 1740, 2243
GlaI GCGC 1 cut(s) 1619
GluI GCNGC 6 cut(s) 424, 445, 465, 545, 1621, 2262
GsuI CTGGAG 1 cut(s) 2297
HaeIII GGCC 3 cut(s) 617, 825, 2108
HhaI GCGC 1 cut(s) 1620
Hin6I GCGC 1 cut(s) 1618
HinP1I GCGC 1 cut(s) 1618
HindIII AAGCTT 1 cut(s) 2252
HinfI GANTC 6 cut(s) 433, 1331, 1483, 1508, 1672, 2231
HphI GGTGA 4 cut(s) 589, 785, 1394, 1854
Hpy166II GTNNAC 6 cut(s) 18, 607, 1289, 1516, 1646, 1989
Hpy8I GTNNAC 6 cut(s) 18, 607, 1289, 1516, 1646, 1989
Hpy99I CGWCG 1 cut(s) 1197
HpyCH4III ACNGT 7 cut(s) 377, 869, 899, 1073, 1123, 1132, 1279
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 378, 1154, 1781, 2006
HpyF3I CTNAG 9 cut(s) 499, 707, 806, 820, 1335, 1552, 1589, 1692, 2250
HspAI GCGC 1 cut(s) 1618
KpnI GGTACC 1 cut(s) 1179
Kzo9I GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
LmnI GCTCC 5 cut(s) 48, 541, 802, 1145, 2188
Lsp1109I GCAGC 6 cut(s) 410, 431, 476, 531, 1632, 2273
LweI GCATC 8 cut(s) 137, 143, 916, 963, 1227, 1231, 1672, 1954
MabI ACCWGGT 1 cut(s) 875
MaeI CTAG 5 cut(s) 959, 1292, 1352, 1740, 2243
MaeIII GTNAC 7 cut(s) 192, 217, 401, 449, 577, 879, 1669
MalI GATC 9 cut(s) 551, 693, 741, 1020, 1056, 1139, 1713, 1872, 2274
MboI GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
MboII GAAGA 7 cut(s) 129, 132, 548, 1040, 1710, 2047, 2312
MfeI CAATTG 2 cut(s) 1297, 1469
MflI RGATCY 3 cut(s) 1018, 1137, 1711
MhlI GDGCHC 3 cut(s) 807, 1150, 2155
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 1666
MmeI TCCRAC 2 cut(s) 711, 1032
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 2 cut(s) 656, 1224
MroXI GAANNNNTTC 1 cut(s) 2108
MscI TGGCCA 1 cut(s) 617
MseI TTAA 7 cut(s) 521, 987, 996, 1217, 1605, 2049, 2338
MslI CAYNNNNRTG 3 cut(s) 563, 1567, 1598
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 857
MspCI CTTAAG 1 cut(s) 520
MspR9I CCNGG 2 cut(s) 877, 1766
MunI CAATTG 2 cut(s) 1297, 1469
Mva1269I GAATGC 1 cut(s) 1967
MvaI CCWGG 2 cut(s) 877, 1766
MwoI GCNNNNNNNGC 5 cut(s) 118, 378, 1154, 1781, 2006
NcoI CCATGG 1 cut(s) 1599
NdeI CATATG 2 cut(s) 1752, 2065
NdeII GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
NlaIV GGNNCC 2 cut(s) 704, 1177
NmuCI GTSAC 5 cut(s) 192, 401, 577, 879, 1669
NsiI ATGCAT 2 cut(s) 656, 1224
NspI RCATGY 1 cut(s) 1652
NspV TTCGAA 1 cut(s) 2298
OliI CACNNNNGTG 1 cut(s) 1567
PaeR7I CTCGAG 1 cut(s) 1925
PceI AGGCCT 2 cut(s) 825, 2108
PciI ACATGT 1 cut(s) 1648
PctI GAATGC 1 cut(s) 1967
PdmI GAANNNNTTC 1 cut(s) 2108
PfeI GAWTC 5 cut(s) 433, 1331, 1483, 1508, 2231
PflFI GACNNNGTC 1 cut(s) 1675
PflMI CCANNNNNTGG 1 cut(s) 1172
PkrI GCNGC 6 cut(s) 425, 446, 466, 546, 1622, 2263
PleI GAGTC 1 cut(s) 1666
PpsI GAGTC 1 cut(s) 1666
PscI ACATGT 1 cut(s) 1648
PshBI ATTAAT 1 cut(s) 1217
Psp124BI GAGCTC 2 cut(s) 807, 2155
Psp6I CCWGG 2 cut(s) 875, 1764
PspGI CCWGG 2 cut(s) 875, 1764
PspN4I GGNNCC 2 cut(s) 704, 1177
PspPI GGNCC 1 cut(s) 18
PspXI VCTCGAGB 1 cut(s) 1925
PstI CTGCAG 5 cut(s) 428, 449, 466, 549, 1786
PsuI RGATCY 3 cut(s) 1018, 1137, 1711
PsyI GACNNNGTC 1 cut(s) 1675
RsaI GTAC 9 cut(s) 5, 250, 303, 313, 663, 1015, 1177, 1445, 1816
RsaNI GTAC 9 cut(s) 4, 249, 302, 312, 662, 1014, 1176, 1444, 1815
RseI CAYNNNNRTG 3 cut(s) 563, 1567, 1598
SacI GAGCTC 2 cut(s) 807, 2155
SaqAI TTAA 7 cut(s) 521, 987, 996, 1217, 1605, 2049, 2338
SatI GCNGC 6 cut(s) 424, 445, 465, 545, 1621, 2262
Sau3AI GATC 9 cut(s) 549, 691, 739, 1018, 1054, 1137, 1711, 1870, 2272
Sau96I GGNCC 1 cut(s) 18
ScaI AGTACT 2 cut(s) 1445, 1816
SchI GAGTC 1 cut(s) 1666
ScrFI CCNGG 2 cut(s) 877, 1766
SduI GDGCHC 3 cut(s) 807, 1150, 2155
SexAI ACCWGGT 1 cut(s) 875
SfaNI GCATC 8 cut(s) 137, 143, 916, 963, 1227, 1231, 1672, 1954
SfcI CTRYAG 8 cut(s) 424, 445, 462, 545, 1122, 1343, 1512, 1782
Sfr274I CTCGAG 1 cut(s) 1925
SfuI TTCGAA 1 cut(s) 2298
SinI GGWCC 1 cut(s) 18
SlaI CTCGAG 1 cut(s) 1925
SmiMI CAYNNNNRTG 3 cut(s) 563, 1567, 1598
SmlI CTYRAG 2 cut(s) 520, 1925
SmoI CTYRAG 2 cut(s) 520, 1925
SseBI AGGCCT 2 cut(s) 825, 2108
SsiI CCGC 2 cut(s) 857, 1895
SspI AATATT 1 cut(s) 472
SspMI CTAG 5 cut(s) 959, 1292, 1352, 1740, 2243
SstI GAGCTC 2 cut(s) 807, 2155
StuI AGGCCT 2 cut(s) 825, 2108
StyD4I CCNGG 2 cut(s) 875, 1764
StyI CCWWGG 2 cut(s) 1599, 2010
TaaI ACNGT 7 cut(s) 377, 869, 899, 1073, 1123, 1132, 1279
TaqI TCGA 5 cut(s) 355, 1195, 1830, 1926, 2298
TatI WGTACW 5 cut(s) 248, 301, 311, 1443, 1814
TfiI GAWTC 5 cut(s) 433, 1331, 1483, 1508, 2231
Tru1I TTAA 7 cut(s) 521, 987, 996, 1217, 1605, 2049, 2338
Tru9I TTAA 7 cut(s) 521, 987, 996, 1217, 1605, 2049, 2338
TscAI CASTG 6 cut(s) 433, 703, 814, 1282, 1468, 1996
TseFI GTSAC 5 cut(s) 192, 401, 577, 879, 1669
TseI GCWGC 6 cut(s) 423, 444, 464, 544, 1620, 2261
Tsp45I GTSAC 5 cut(s) 192, 401, 577, 879, 1669
TspDTI ATGAA 3 cut(s) 1082, 1125, 1259
TspGWI ACGGA 3 cut(s) 950, 1343, 1744
TspRI CASTG 6 cut(s) 433, 703, 814, 1282, 1468, 1996
Tth111I GACNNNGTC 1 cut(s) 1675
Van91I CCANNNNNTGG 1 cut(s) 1172
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 18
VspI ATTAAT 1 cut(s) 1217
XagI CCTNNNNNAGG 2 cut(s) 1832, 1925
XapI RAATTY 4 cut(s) 1181, 1246, 1838, 2085
XceI RCATGY 1 cut(s) 1652
XcmI CCANNNNNNNNNTGG 3 cut(s) 475, 578, 750
XhoI CTCGAG 1 cut(s) 1925
XmiI GTMKAC 1 cut(s) 1515
XmnI GAANNNNTTC 1 cut(s) 2108
XspI CTAG 5 cut(s) 959, 1292, 1352, 1740, 2243
ZrmI AGTACT 2 cut(s) 1445, 1816
Zsp2I ATGCAT 2 cut(s) 656, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.