Rw5G042870

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001222
Physical Location & Seq
Forward (+)
74927905 .. 74935059
7155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042870.1

Sequence Viewer

Length: 2748 bp
ATGTATTGGACTGTAGGGCTGGGATTGCTGATGAACAAAGTGAAGGTTTGTCGATCATTGAAAGGACCTCCCGACAAAGCTTCAATGGAACTTCTTCTGCCAAATCTGATATTAGCAATGATGGGAATTTGTGTTACTAAATCCAAAGGCAGAAGAACAGCAAATTGGCTACCTGTGGAACTTCATAAAGAGTCTTGCCCTAGTGCTGTTGATAAGTACATGTGTAAGGGATGGCTGCCTTCAGGGGAGTTACAAACAGCTGTTAGGAAGCATCTTTTATATTTGGGTTGGAAAGTAGAGTATATGATAGATAAGGGCAGAATGAGATACCGGTACTTGTCGCCTCATGGAGAACCCGAATACTCTCTAATTAAAGTGTGCAGTAACTTGAGAAAGTCCTTGAAAGATTCACAATTTGCAATCTCTCAAGATTCACCTCAGTGTTTGCATGGTTCACCTGGGCAACTTCTTGTCACTGAAGAACCACAAGAGATTCAGCATCCTAATTATTGTCCTCAACAGGTAGCGTCTCCCTTTTCCAATTCCAAGTTACATCCCAAGGTATTTATTTACAAACCCGAATATTGCCCTCAAGCTGTTGTGGCATATGTAGACTCTCCCATAAGGGGACCCAGAAGTTTGAGAGTAAAAACAATGCGGTCTAAAGCAAAGAAGCACCTCTCTGCTGTGGGGTGGGAGTTCTACTACTCAGAAATAAGATCATCAAATAGCTTGCGCTTCAAATCTCCCAATGGAAATGTATACAACTCACTTCTGGATGCCTGCAAAGTCTGTATGGATGAAGAAAATTCTGAAAGAAGACCTGCTGAATGCAGGTATGTCATTGACGAAGATGAGGCCCACTTAATTAAGAAAAAAATATTTTCTGTAGCAAATAGAAAATGCAAAAGGAATAGAATTTGTAGCTTGTTAAACAGTGGACCTCACTTGCTTCACGAATGCCCAAAGGATCAATGTGCTAACCCTCCAAAGTTGAAAAGGCGAAAGGCATCTGCATCTTCCTCTGGGTTTAGAAATGGTTCTGGTGTTAGTCAGCCAACTCGTGTGCTCAGGTCAAGCAAACGAGTCCAGGAAGTGGTTACTCCTACTTCCACACACCAGAATCCCCGCACTGTCTTATCTTGGTTGATAGACAATAATGTGGTTTTGCCAAGGGAAAAAGTACATTATTGTAGCAGAAAGGGTGGTCCTTCAATGGCAGAAGGGAAAATAAGTAGAGAGGGGATCAAGTGCAGCTGTTGCCAAAAGGTGTTTACTCTTAGCTGCTTTGAGAGTCATGCTGGCTTCAATGAGACTCATGCTGGCTGCAATAACCACAGACCTGCTTCCAATATTTTTCTGGATGATGGTAGGTCTCTCTTGGATTGCCAGATGCAAATTATACTTGAAAGAAGGAAGAGGACTTGCAGAACAGAACCACGCCATAGGATGAAGGGAAATCAAGTTCGAGGCAAGAGTGATTACATATGTACTGTGTGTCACTATGGTGGTGATTTAATCTTATGTGACGAATGTCCGTCATCATTTCACAAGAGTTGCGTTGGGTTGAAGGATGTTCCAGATGGTGAGTGGTTCTGTCCATCTTGCTGTTGTGGATTATGTGGCCAAAGAAAACTCAAAGAAGACAAAGAACCTATTATGCATTCTGATTTTCTCACATGTGGTCAATGCGAGCACAAATATCACACTGGGTGCCTGAGAAAAGGAGGTGTGGATATGTCGGAAAGTGATTCAAAAGGAAATTGGTTTTGCAGCAAGAATTGTAAAAAGATATCTTTGGGCCTCCACAAGCTTTTAGGGAAACAATTTCCGGCGGGTGTTGGCAATTTAACTTGGTCACTTCTGAAGTCCATGAAATCTGATAGTGATGATGCTACTGAACCTGACAATGATGCCATAACGGAGAGTTTTAGCAGGCTTAGTATTGCCCTTGATGTGATGCACGAGTGTTTTGAGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGCAGAAGATATCATCTTCAGTAGAGGGTCAAATCTCAATCGCTTGAACTTTCAAGGGTTCTATACCATGCTATTGGAAAGAAATGATGAGCTAATCACTGTGGCCACTGTAAGGATCTATGGAAAGAAGGTGGCCGAAGTACCTCTTGTTGCTACCAGATTTCAATATCGTCGACTTGGAATGTGTCGTGTGTTGATGAATTTACTTGAAAAGATGCTTATGGATTTAGGTGTGGAGAGATTGGTTTTGCCCGCTGTCCCCAGTGTGCTAAACACATGGACTACTGCATTTGGGTTTTCAAGAATGACAGAGTCTGAGAGGCTACAGTTTCTGGATCATACTTTCCTGGATTTTCAGGATACCATTATGTGTCAGAAACTTTTAATGAAGATCTCTCCAGCTGAACCAAGCCTGTTGAAAGGAACACAGCCTAATATTTCTGGAAGTGGTGATAATATTGATCTTGATGGGTCCAGTTCTGTCTCTGAAGTGTGTCAACTGGAACAAACAGAGGATAGCCGAACCGTATTCCAGGGACTTGAAAGTGCTGCAGGGAAAAGGAGAGCATGCGTTGATAAGAACTGTCCAGAGTTCAAGCATACAAAGAGAAATAGCATGAAGATATACTGTGCATATGATCATGATGACTATTTTGGTTATAACATCTGGGAGGAAGACAAAGAAGGGAAGAGTGAATTAGACACGAAGAGAAAGTTGGTTTCAAACTTCATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

915

Amino Acids

103.63

Weight (kDa)

8.89

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 59 - 132 1.3e-20 Domain of unknown function (DUF7028)
DUF7028 PF22970 191 - 269 6.6e-24 Domain of unknown function (DUF7028)
TDBD PF16135 409 - 466 7.5e-18 Tify domain binding domain
PHD-1st_NSD PF23011 492 - 536 8.9e-08 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 497 - 536 3.5e-08 PHD-finger
IDM1_C PF23209 658 - 796 4.8e-55 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2670
Acc36I ACCTGC 3 cut(s) 825, 832, 1351
AccB1I GGYRCC 1 cut(s) 1713
AccB7I CCANNNNNTGG 1 cut(s) 1096
AccI GTMKAC 3 cut(s) 612, 762, 2182
AciI CCGC 4 cut(s) 658, 1129, 1835, 2262
AclWI GGATC 4 cut(s) 978, 1253, 2132, 2352
AcoI YGGCCR 3 cut(s) 1624, 2112, 2142
AcsI RAATTY 4 cut(s) 126, 808, 918, 2209
AcuI CTGAAG 5 cut(s) 225, 498, 1886, 2011, 2517
AdeI CACNNNGTG 1 cut(s) 1713
AfaI GTAC 5 cut(s) 218, 335, 1185, 1492, 2151
AfiI CCNNNNNNNGG 3 cut(s) 626, 1096, 2121
AflIII ACRYGT 2 cut(s) 219, 1679
AgeI ACCGGT 1 cut(s) 330
AjnI CCWGG 5 cut(s) 457, 1089, 1997, 2355, 2541
AjuI GAANNNNNNNTTGG 4 cut(s) 148, 180, 2708, 2740
AleI CACNNNNGTG 2 cut(s) 439, 1506
AloI GAACNNNNNNTCC 2 cut(s) 1449, 1481
Alw21I GWGCWC 2 cut(s) 1071, 1698
Alw26I GTCTC 4 cut(s) 534, 1307, 1380, 2497
AlwI GGATC 4 cut(s) 978, 1253, 2132, 2352
AlwNI CAGNNNCTG 2 cut(s) 2324, 2341
AoxI GGCC 5 cut(s) 858, 1624, 1801, 2112, 2142
ApeKI GCWGC 6 cut(s) 235, 1254, 1284, 1326, 1773, 2558
ApoI RAATTY 4 cut(s) 126, 808, 918, 2209
AsiGI ACCGGT 1 cut(s) 330
Asp700I GAANNNNTTC 2 cut(s) 93, 1039
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 7 cut(s) 65, 629, 859, 941, 1208, 1801, 2481
AsuHPI GGTGA 5 cut(s) 426, 447, 1523, 1598, 2471
AvaII GGWCC 5 cut(s) 65, 629, 941, 1208, 2481
BalI TGGCCA 2 cut(s) 1626, 2114
BanI GGYRCC 1 cut(s) 1713
BauI CACGAG 2 cut(s) 1062, 1964
BbsI GAAGAC 3 cut(s) 826, 1650, 2691
Bbv12I GWGCWC 2 cut(s) 1071, 1698
BbvI GCAGC 6 cut(s) 222, 1266, 1271, 1313, 1785, 2545
BccI CCATC 6 cut(s) 115, 225, 1361, 1577, 1609, 2471
BciT130I CCWGG 5 cut(s) 459, 1091, 1999, 2357, 2543
BciVI GTATCC 1 cut(s) 2362
BclI TGATCA 1 cut(s) 2647
BcoDI GTCTC 4 cut(s) 534, 1307, 1380, 2497
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 4 cut(s) 12, 888, 2333, 2559
BfuAI ACCTGC 3 cut(s) 825, 832, 1351
BfuI GTATCC 1 cut(s) 2362
BglII AGATCT 2 cut(s) 2005, 2400
BisI GCNGC 6 cut(s) 236, 1255, 1285, 1327, 1774, 2559
BlsI GCNGC 6 cut(s) 237, 1256, 1286, 1328, 1775, 2560
Bme1390I CCNGG 5 cut(s) 459, 1091, 1999, 2357, 2543
Bme18I GGWCC 5 cut(s) 65, 629, 941, 1208, 2481
BmgT120I GGNCC 7 cut(s) 65, 629, 859, 941, 1208, 1801, 2481
BmiI GGNNCC 5 cut(s) 630, 631, 1715, 1989, 2482
BmrFI CCNGG 5 cut(s) 459, 1091, 1999, 2357, 2543
BmrI ACTGGG 2 cut(s) 1719, 2265
BmuI ACTGGG 2 cut(s) 1719, 2265
BoxI GACNNNNGTC 1 cut(s) 1533
BpiI GAAGAC 3 cut(s) 826, 1650, 2691
BpmI CTGGAG 1 cut(s) 2391
Bpu10I CCTNAGC 1 cut(s) 1070
BpuEI CTTGAG 3 cut(s) 409, 411, 576
BsaI GGTCTC 1 cut(s) 1380
BsaJI CCNNGG 4 cut(s) 458, 558, 1172, 2542
BsaWI WCCGGW 1 cut(s) 330
Bsc4I CCNNNNNNNGG 3 cut(s) 626, 1096, 2121
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 4 cut(s) 1714, 2271, 2484, 2514
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 5 cut(s) 459, 1091, 1999, 2357, 2543
BseDI CCNNGG 4 cut(s) 458, 558, 1172, 2542
BseGI GGATG 8 cut(s) 236, 499, 553, 784, 805, 1369, 1455, 1579
BseLI CCNNNNNNNGG 3 cut(s) 626, 1096, 2121
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 5 cut(s) 452, 723, 1084, 1709, 2316
BseNI ACTGG 4 cut(s) 1714, 2271, 2484, 2514
BseXI GCAGC 6 cut(s) 222, 1266, 1271, 1313, 1785, 2545
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 2 cut(s) 400, 1273
BshFI GGCC 5 cut(s) 860, 1626, 1803, 2114, 2144
BshNI GGYRCC 1 cut(s) 1713
BshTI ACCGGT 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 1071, 1698
BsiSI CCGG 2 cut(s) 331, 1832
BslFI GGGAC 3 cut(s) 642, 2252, 2559
BslI CCNNNNNNNGG 3 cut(s) 626, 1096, 2121
BsmAI GTCTC 4 cut(s) 534, 1307, 1380, 2497
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 3 cut(s) 642, 2252, 2559
BsmI GAATGC 3 cut(s) 836, 965, 1663
BsnI GGCC 5 cut(s) 860, 1626, 1803, 2114, 2144
Bso31I GGTCTC 1 cut(s) 1380
Bsp1286I GDGCHC 2 cut(s) 1071, 1698
BspACI CCGC 4 cut(s) 658, 1129, 1835, 2262
BspANI GGCC 5 cut(s) 860, 1626, 1803, 2114, 2144
BspCNI CTCAG 5 cut(s) 451, 722, 1083, 1710, 2317
BspHI TCATGA 1 cut(s) 2650
BspLI GGNNCC 5 cut(s) 630, 631, 1715, 1989, 2482
BspMAI CTGCAG 1 cut(s) 2563
BspMI ACCTGC 3 cut(s) 825, 832, 1351
BspPI GGATC 4 cut(s) 978, 1253, 2132, 2352
BspT107I GGYRCC 1 cut(s) 1713
BspTNI GGTCTC 1 cut(s) 1380
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 4 cut(s) 1714, 2271, 2484, 2514
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 4 cut(s) 458, 558, 1172, 2542
BssNAI GTATAC 1 cut(s) 763
BssSI CACGAG 2 cut(s) 1062, 1964
BssT1I CCWWGG 2 cut(s) 558, 1172
Bst1107I GTATAC 1 cut(s) 763
Bst2BI CACGAG 2 cut(s) 1062, 1964
Bst2UI CCWGG 5 cut(s) 459, 1091, 1999, 2357, 2543
Bst6I CTCTTC 3 cut(s) 1412, 2693, 2711
BstAPI GCANNNNNTGC 1 cut(s) 1260
BstC8I GCNNGC 8 cut(s) 734, 784, 1303, 1324, 1694, 1937, 2262, 2578
BstDEI CTNAG 7 cut(s) 438, 709, 1070, 1280, 1718, 1940, 2325
BstF5I GGATG 8 cut(s) 236, 499, 553, 784, 805, 1369, 1455, 1579
BstHHI GCGC 1 cut(s) 738
BstMAI GTCTC 4 cut(s) 534, 1307, 1380, 2497
BstMWI GCNNNNNNNGC 3 cut(s) 25, 602, 1260
BstNI CCWGG 5 cut(s) 459, 1091, 1999, 2357, 2543
BstNSI RCATGY 3 cut(s) 223, 1683, 2580
BstPAI GACNNNNGTC 1 cut(s) 1533
BstSCI CCNGG 5 cut(s) 457, 1089, 1997, 2355, 2541
BstSFI CTRYAG 4 cut(s) 12, 888, 2333, 2559
BstV1I GCAGC 6 cut(s) 222, 1266, 1271, 1313, 1785, 2545
BstV2I GAAGAC 3 cut(s) 826, 1650, 2691
BstX2I RGATCY 3 cut(s) 2005, 2124, 2400
BstXI CCANNNNNNTGG 1 cut(s) 2083
BstYI RGATCY 3 cut(s) 2005, 2124, 2400
BstZ17I GTATAC 1 cut(s) 763
BsuI GTATCC 1 cut(s) 2362
BsuRI GGCC 5 cut(s) 860, 1626, 1803, 2114, 2144
BtsCI GGATG 8 cut(s) 236, 499, 553, 784, 805, 1369, 1455, 1579
BtsIMutI CAGTG 8 cut(s) 446, 474, 943, 1131, 1707, 2106, 2115, 2278
BveI ACCTGC 3 cut(s) 825, 832, 1351
Cac8I GCNNGC 8 cut(s) 734, 784, 1303, 1324, 1694, 1937, 2262, 2578
CaiI CAGNNNCTG 2 cut(s) 2324, 2341
CciI TCATGA 1 cut(s) 2650
CfoI GCGC 1 cut(s) 738
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 7 cut(s) 65, 629, 859, 941, 1208, 1801, 2481
CseI GACGC 1 cut(s) 516
Csp6I GTAC 5 cut(s) 217, 334, 1184, 1491, 2150
CspAI ACCGGT 1 cut(s) 330
CviQI GTAC 5 cut(s) 217, 334, 1184, 1491, 2150
DdeI CTNAG 7 cut(s) 438, 709, 1070, 1280, 1718, 1940, 2325
DraIII CACNNNGTG 1 cut(s) 1713
EaeI YGGCCR 3 cut(s) 1624, 2112, 2142
Eam1104I CTCTTC 3 cut(s) 1412, 2693, 2711
EarI CTCTTC 3 cut(s) 1412, 2693, 2711
Eco130I CCWWGG 2 cut(s) 558, 1172
Eco31I GGTCTC 1 cut(s) 1380
Eco32I GATATC 2 cut(s) 1794, 2020
Eco47I GGWCC 5 cut(s) 65, 629, 941, 1208, 2481
Eco57I CTGAAG 5 cut(s) 225, 498, 1886, 2011, 2517
EcoO109I RGGNCCY 2 cut(s) 65, 629
EcoRII CCWGG 5 cut(s) 457, 1089, 1997, 2355, 2541
EcoRV GATATC 2 cut(s) 1794, 2020
EcoT14I CCWWGG 2 cut(s) 558, 1172
EcoT22I ATGCAT 1 cut(s) 1665
ErhI CCWWGG 2 cut(s) 558, 1172
Esp3I CGTCTC 1 cut(s) 534
FalI AAGNNNNNCTT 2 cut(s) 2139, 2171
FaqI GGGAC 3 cut(s) 642, 2252, 2559
FauI CCCGC 3 cut(s) 1136, 1828, 2269
FauNDI CATATG 3 cut(s) 607, 1487, 2644
FbaI TGATCA 1 cut(s) 2647
FblI GTMKAC 3 cut(s) 612, 762, 2182
Fnu4HI GCNGC 6 cut(s) 236, 1255, 1285, 1327, 1774, 2559
FokI GGATG 8 cut(s) 243, 486, 540, 791, 812, 1376, 1462, 1586
Fsp4HI GCNGC 6 cut(s) 236, 1255, 1285, 1327, 1774, 2559
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 737
GluI GCNGC 6 cut(s) 236, 1255, 1285, 1327, 1774, 2559
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 1 cut(s) 2391
HaeIII GGCC 5 cut(s) 860, 1626, 1803, 2114, 2144
HapII CCGG 2 cut(s) 331, 1832
HgaI GACGC 1 cut(s) 516
HhaI GCGC 1 cut(s) 738
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 2 cut(s) 2183, 2507
HindII GTYRAC 2 cut(s) 2183, 2507
HindIII AAGCTT 2 cut(s) 78, 1811
HpaII CCGG 2 cut(s) 331, 1832
HphI GGTGA 5 cut(s) 426, 447, 1523, 1598, 2471
Hpy166II GTNNAC 7 cut(s) 455, 613, 763, 941, 1275, 2183, 2507
Hpy8I GTNNAC 7 cut(s) 455, 613, 763, 941, 1275, 2183, 2507
Hpy99I CGWCG 1 cut(s) 2184
HpyAV CCTTC 9 cut(s) 37, 249, 1217, 1221, 1407, 1447, 1564, 2131, 2687
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 602, 1260
HpyF3I CTNAG 7 cut(s) 438, 709, 1070, 1280, 1718, 1940, 2325
HspAI GCGC 1 cut(s) 736
KflI GGGWCCC 1 cut(s) 629
Ksp22I TGATCA 1 cut(s) 2647
Lsp1109I GCAGC 6 cut(s) 222, 1266, 1271, 1313, 1785, 2545
MaeI CTAG 1 cut(s) 201
MaeIII GTNAC 9 cut(s) 133, 249, 383, 472, 549, 1099, 1499, 1526, 1857
MflI RGATCY 3 cut(s) 2005, 2124, 2400
MhlI GDGCHC 2 cut(s) 1071, 1698
MlsI TGGCCA 2 cut(s) 1626, 2114
MluNI TGGCCA 2 cut(s) 1626, 2114
MlyI GAGTC 6 cut(s) 200, 608, 1095, 1303, 1309, 2330
MmeI TCCRAC 2 cut(s) 269, 1722
Mox20I TGGCCA 2 cut(s) 1626, 2114
Mph1103I ATGCAT 1 cut(s) 1665
MroXI GAANNNNTTC 2 cut(s) 93, 1039
MscI TGGCCA 2 cut(s) 1626, 2114
MseI TTAA 8 cut(s) 372, 866, 870, 932, 1517, 1850, 1983, 2393
MslI CAYNNNNRTG 2 cut(s) 439, 1506
Msp20I TGGCCA 2 cut(s) 1626, 2114
MspA1I CMGCKG 4 cut(s) 260, 1257, 2264, 2411
MspI CCGG 2 cut(s) 331, 1832
MspR9I CCNGG 5 cut(s) 459, 1091, 1999, 2357, 2543
Mva1269I GAATGC 3 cut(s) 836, 965, 1663
MvaI CCWGG 5 cut(s) 459, 1091, 1999, 2357, 2543
MwoI GCNNNNNNNGC 3 cut(s) 25, 602, 1260
NdeI CATATG 3 cut(s) 607, 1487, 2644
NlaIV GGNNCC 5 cut(s) 630, 631, 1715, 1989, 2482
NmuCI GTSAC 4 cut(s) 472, 1499, 1526, 1857
NsiI ATGCAT 1 cut(s) 1665
NspI RCATGY 3 cut(s) 223, 1683, 2580
OliI CACNNNNGTG 2 cut(s) 439, 1506
PacI TTAATTAA 1 cut(s) 870
PaeI GCATGC 1 cut(s) 2580
PagI TCATGA 1 cut(s) 2650
PciI ACATGT 2 cut(s) 219, 1679
PctI GAATGC 3 cut(s) 836, 965, 1663
PdmI GAANNNNTTC 2 cut(s) 93, 1039
PfeI GAWTC 5 cut(s) 407, 431, 493, 1123, 1751
PflFI GACNNNGTC 1 cut(s) 2320
PflMI CCANNNNNTGG 1 cut(s) 1096
PfoI TCCNGGA 2 cut(s) 1089, 2355
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 6 cut(s) 237, 1256, 1286, 1328, 1775, 2560
PleI GAGTC 6 cut(s) 199, 608, 1094, 1302, 1309, 2329
PpsI GAGTC 6 cut(s) 199, 608, 1094, 1302, 1309, 2329
PpuMI RGGWCCY 2 cut(s) 65, 629
PscI ACATGT 2 cut(s) 219, 1679
PshAI GACNNNNGTC 1 cut(s) 1533
PsiI TTATAA 1 cut(s) 2670
Psp5II RGGWCCY 2 cut(s) 65, 629
Psp6I CCWGG 5 cut(s) 457, 1089, 1997, 2355, 2541
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 5 cut(s) 457, 1089, 1997, 2355, 2541
PspN4I GGNNCC 5 cut(s) 630, 631, 1715, 1989, 2482
PspPI GGNCC 7 cut(s) 65, 629, 859, 941, 1208, 1801, 2481
PspPPI RGGWCCY 2 cut(s) 65, 629
PstI CTGCAG 1 cut(s) 2563
PstNI CAGNNNCTG 2 cut(s) 2324, 2341
PsuI RGATCY 3 cut(s) 2005, 2124, 2400
PsyI GACNNNGTC 1 cut(s) 2320
PvuII CAGCTG 3 cut(s) 260, 1257, 2411
RsaI GTAC 5 cut(s) 218, 335, 1185, 1492, 2151
RsaNI GTAC 5 cut(s) 217, 334, 1184, 1491, 2150
RseI CAYNNNNRTG 2 cut(s) 439, 1506
SalI GTCGAC 1 cut(s) 2181
SaqAI TTAA 8 cut(s) 372, 866, 870, 932, 1517, 1850, 1983, 2393
SatI GCNGC 6 cut(s) 236, 1255, 1285, 1327, 1774, 2559
Sau96I GGNCC 7 cut(s) 65, 629, 859, 941, 1208, 1801, 2481
SchI GAGTC 6 cut(s) 200, 608, 1095, 1303, 1309, 2330
ScrFI CCNGG 5 cut(s) 459, 1091, 1999, 2357, 2543
SduI GDGCHC 2 cut(s) 1071, 1698
SfcI CTRYAG 4 cut(s) 12, 888, 2333, 2559
SinI GGWCC 5 cut(s) 65, 629, 941, 1208, 2481
SmiMI CAYNNNNRTG 2 cut(s) 439, 1506
SmlI CTYRAG 3 cut(s) 388, 426, 591
SmoI CTYRAG 3 cut(s) 388, 426, 591
SphI GCATGC 1 cut(s) 2580
SsiI CCGC 4 cut(s) 658, 1129, 1835, 2262
SspI AATATT 5 cut(s) 584, 882, 1354, 2446, 2467
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 5 cut(s) 457, 1089, 1997, 2355, 2541
StyI CCWWGG 2 cut(s) 558, 1172
TaqI TCGA 3 cut(s) 52, 1468, 2182
TatI WGTACW 3 cut(s) 216, 1183, 1490
TfiI GAWTC 5 cut(s) 407, 431, 493, 1123, 1751
Tru1I TTAA 8 cut(s) 372, 866, 870, 932, 1517, 1850, 1983, 2393
Tru9I TTAA 8 cut(s) 372, 866, 870, 932, 1517, 1850, 1983, 2393
TscAI CASTG 8 cut(s) 446, 481, 943, 1138, 1714, 2113, 2122, 2278
TseFI GTSAC 4 cut(s) 472, 1499, 1526, 1857
TseI GCWGC 6 cut(s) 235, 1254, 1284, 1326, 1773, 2558
Tsp45I GTSAC 4 cut(s) 472, 1499, 1526, 1857
TspDTI ATGAA 9 cut(s) 47, 173, 816, 1466, 1889, 2222, 2411, 2642, 2728
TspGWI ACGGA 2 cut(s) 1527, 1937
TspRI CASTG 8 cut(s) 446, 481, 943, 1138, 1714, 2113, 2122, 2278
Tth111I GACNNNGTC 1 cut(s) 2320
Van91I CCANNNNNTGG 1 cut(s) 1096
VpaK11BI GGWCC 5 cut(s) 65, 629, 941, 1208, 2481
XapI RAATTY 4 cut(s) 126, 808, 918, 2209
XceI RCATGY 3 cut(s) 223, 1683, 2580
XcmI CCANNNNNNNNNTGG 2 cut(s) 1357, 1587
XmiI GTMKAC 3 cut(s) 612, 762, 2182
XmnI GAANNNNTTC 2 cut(s) 93, 1039
XspI CTAG 1 cut(s) 201
Zsp2I ATGCAT 1 cut(s) 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.