Rroxscaffold_1G00010940

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001222
Physical Location & Seq
Reverse (-)
13959898 .. 13966732
6835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00010940.1

Sequence Viewer

Length: 3585 bp
ATGGCGGTGGCCATGGCCCTTGAAGAACAGGGCCGGCGTGTTAATAAGAGAAACCATAATTTGAGACAAAATCAAGGGAAGTTACTTTTTGGTGCCAAAGTTGAGGTGAGGAGTGAAGAAGAAGGGTTTCAGGGCTCATGGCACCCTGGGACTGTCGCTGCATGCACCAAAGGTTGTCGTAAAGTAGAATACGATCATATTCTACGTGATGATGAGTCTGCTAATCTTGTGGATGCTGTTTATGTTTCTCCCATATTGGATGCCATTGATTCTGTAACTGATGAGCAGAGTAATTACCATCGCGGAAGGATAAGGCCAAAGCCGCCTAAGATTGAGGTAGGGGCATGGGGTCTTCCCTATGGACTCTGTGTGGATGTGTATCATCAGGATGCATGGTGGGAAGGTGTGGTTTTTGATCATGAAGATGGTTCAGAAAAGAGGAAGATCTTCTTTCCGGATTTGGGAGATGAACTAACAACTGGAATTGACACTCTAAGAATTACTCAGGACTGGGATGAGGTTACAGAGAATTGGCAGCGACGTGGGACTTGGATATTTCTTGAGTTGATTGAACAATATGAGCAAGAGAGGTACATTGCTGTCTCTGTGAAGCAAGTTTGGTATGATGTTCGATTAAAAGAGGGCTTTGAGAAGGTCAGGCAGTGGACTTCTCCAATGAGATATCTATGGGAGGAGCTGGTGTTGGAGGTCATTGATGATAACTTAAGCATCACCGCGGATGAACTTTTTCGGGTGTTAGAAGTGTCAGGATGCTTGTCAAGAGGATTCCAGGTAGAGTTTGAGTCAGCTCAATGTGTCTTAGATGCTGATATGAATTCTAAATCATATACCACACACTCTCTTGCTATTGTACCTGTTGGCAACACATTGGATGGTAATTTGTTAGTTGATCATGATGATTTTATGAAGAATGTATTGGACTGTAGGGCTGGGATTGCTGATGAACAAAGTGAAGGTTTGTCGATCATTGAAAGGACCTCCCGACAAAACTTCAATGGAACTTCTTCTGCCAAATCTGATATTAGCAGTGATGGAATTTGTGTTACTAAATCCAAAGGCAGAAGAACAGCAAATTGGCTACATGTGGAACTTCATAAAGAGTCTTGCCCTGGTGCTGTTGATAAGTACTTGGGTAAGGGACGGCTGCCTTCAGGGGAGTTACAAACAGCTGTTAGGAAGCATCTTTTATATTTGGGTTGGAAAGTAGAGTATATGATAGATAAGGGCAGAATGAGATACCGGTACTTGTCGCCTGATGGAGAATCCGAATACTCTCTTATTAAAGTGTGCAGTAACTTGAGAAAGTCCTCGAAAGATTCACAATTTGCAATCTCTCAAGATGCACCTCAGTGTTTGCATGGTTCACCTGGACAGCTTCTTGTCACTGAAGAACCACAAGAGATTCAGCATCCTAATTATTGTCCTCAACAGGTAGCGTCACCTTTTTCCAATTCCATGTTACATCCCGAGGTATTTATTTACAAACCCGAATATTGCCCTCAAGCAGTTGTGGCATATGTAGATTCTCCCATAAGGGGACCCAGAAGTTTGAGAGCAAAAACAATGCGGTCTAAAGCAAAGAAGCACCTCTCTGCTGTGGGGTGGGAGTTCTACTACTCAGAAATAAGATCATCAAATAGCTTGCGCTTCAAATCTCCCAATGGAAATGTATACAACTCACTTCTGGATGCCTGCAAAGTCTGTATGGATGAAGAAAATTCTGAAAGAAGACCTGCTGAATGCAGGTATGTCATTGACGAAGATGAGGCCCACTTAATAAAGAAAAAAATATTTTCTGTAGCAAATAGAAAACGCAAAAGTAATAGAATTTATAGCTTGTTAAACAGTGGACCTCACTTGCTTCACGAATGCCCAAAGGATCAATGTGCTAACCCTCCAAAGTTGAAAAGGCGAAAGGCATCTGCATCTTCCTCTGGGTTTAGAAATGGTTTTGGTGTTAGTCAGCCAACTCGTGTGCTCAGGTCAAGCAAACGAGTCCAGGAAGTGGTTACTCCTACTTCCACACACCAGAATCCCCGCACTGTCTTATCTTGGTTGATAGACAATAATGTGGTTTTGCCAAGGGAAAAAGTACATTATTGTAGCAGAAAGGGTGGTCCTTCAATGGCAGAAGGGAAAATAAGTAGAGAGGGGATCAAGTGCAGCTGTTGCCAAAAGGTGTTTACTCTTAGCTGCTTTGAGAGTCATGCTGGCTTCAATGAGACTCATGCTGGCTGCAGTAACCACAGACCTGCTTCCAATATTTTTCTGGATGATGGTAGGTCTCTCTTGGATTGCCAGATGCAAATTATACTCGAAAGAAGGAAGAGGATTTGCAGAACAGAACCACGCCATAGGATGAAGGGAAATCAAGTTCGAGGCGAGAGTGATTACATATGTACTGTGTGTCACTATGGTGGTGATTTAATCTTATGTGACGAATGTCCATCATCATTTCACAAGAGTTGCGTTGGGTTGAAGGATGTTCCAGATGGTGAGTGGTTCTGTCCATCTTGCTGTTGTGGATTTTGTGGCCAAAGAAAACTCAAAGAAGACAAAGATCCTATTATGTATTCTGATTTTGTCACATGCGGTCAATGCGAGCACAAATATCACACTGGGTGCCTGAGAAAAGGAGGTGTGGATATGTCGGAAAGTGATTCAAAGGGAAATTGGTTTTGCAGCAAGAATTGTAAAAAGATATCTTTGGGCCTCCACAAGCTTTTAGGGAAACAATTTCCAGCGGGTGTTGGCAATTTAACTTGGTCACTTCTGAAGTCCATGAAATCTGATAGTGATGATGCTACTGAACCTGACAATGATGCCATAACGGAGAGTTTTAGCAGGCTTAGTATTGCCCTTGATGTGATGCACGAGTGTTTTGAGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGCAGAAGATATCATCTTCAGTAGAGGGTCAAATCTTAATCGCTTGAACTTTCAAGGGTTCTATACCATGCTATTGGAAAGAAATGATGAGCTAATCACTGTGGCTACTGTAAGGATCTACGGAAAGAAGGTGGCCGAAGTACCTCTTGTTGCTACCAGATTTCAATATCGTCGACTTGGAATGTGTCGTGTGTTGATGAATTTACTTGAAAAGATGCTTATGGATTTAGGTGTGGAGAGATTGGTTTTGCCTGCTGTCCCCAGTGTGCTAAACACATGGACTACTGCATTTGGGTTTTCAAGAATGACAGAGTCTGAGAGGCTACAGTTTCTGGATCATACTTTCCTGGATTTTCAGGATACCATTATGTGTCAGAAACTTTTAATGAAGATCTCTCCAGCTGAACCAAGCCTGTTGAAAGGAACACAGCCTAATATTTCTGGAAGTGATGATAATATTGATCTTGATGGGTCCAGTTCTGTCTCTGAAGTGTGTCAACTGGAACAAACAGAGGATAGCCAAACCGTATTCCAGGGACTTGAAAGTGCTGCAGGGAAAAGGAGAGCATGCGTTGATAAGAACTGTCCAGAGTTCAAGCAGTACAAGCGAATAAGGAAATCAAATTTGGCTGCCATAGTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

1194

Amino Acids

135.25

Weight (kDa)

7.27

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 31 - 109 1.1e-13 Agenet domain
DUF7028 PF22970 369 - 443 2.5e-21 Domain of unknown function (DUF7028)
DUF7028 PF22970 501 - 579 9.4e-24 Domain of unknown function (DUF7028)
TDBD PF16135 719 - 776 1.1e-17 Tify domain binding domain
PHD-1st_NSD PF23011 804 - 846 6.8e-07 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 807 - 846 4.7e-08 PHD-finger
IDM1_C PF23209 968 - 1106 7e-55 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1755, 1762, 2281
AccB1I GGYRCC 3 cut(s) 92, 141, 2643
AccB7I CCANNNNNTGG 1 cut(s) 2026
AccI GTMKAC 2 cut(s) 1692, 3112
AccII CGCG 2 cut(s) 303, 737
AccIII TCCGGA 1 cut(s) 454
AciI CCGC 9 cut(s) 5, 303, 323, 735, 737, 1588, 2059, 2613, 2765
AclWI GGATC 5 cut(s) 1908, 2183, 2576, 3062, 3282
AcoI YGGCCR 3 cut(s) 9, 2554, 3072
AcsI RAATTY 6 cut(s) 835, 1056, 1738, 1848, 3139, 3562
AcuI CTGAAG 5 cut(s) 1155, 1428, 2816, 2941, 3447
AdeI CACNNNGTG 1 cut(s) 2643
AfaI GTAC 8 cut(s) 593, 873, 1148, 1265, 2115, 2422, 3081, 3542
AfiI CCNNNNNNNGG 3 cut(s) 461, 1556, 2026
AflII CTTAAG 1 cut(s) 724
AflIII ACRYGT 1 cut(s) 1102
AgeI ACCGGT 1 cut(s) 1260
AjiI CACGTC 1 cut(s) 542
AjnI CCWGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
AjuI GAANNNNNNNTTGG 6 cut(s) 920, 952, 1078, 1110, 3548, 3580
AleI CACNNNNGTG 2 cut(s) 1369, 2436
AloI GAACNNNNNNTCC 2 cut(s) 2379, 2411
Alw21I GWGCWC 2 cut(s) 2001, 2628
Alw26I GTCTC 5 cut(s) 58, 607, 2237, 2310, 3427
AlwI GGATC 5 cut(s) 1908, 2183, 2576, 3062, 3282
AlwNI CAGNNNCTG 2 cut(s) 3254, 3271
Ama87I CYCGRG 1 cut(s) 1487
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 8 cut(s) 9, 15, 31, 314, 1788, 2554, 2731, 3072
ApeKI GCWGC 9 cut(s) 158, 535, 1165, 2184, 2214, 2256, 2703, 3488, 3569
ApoI RAATTY 6 cut(s) 835, 1056, 1738, 1848, 3139, 3562
AsiGI ACCGGT 1 cut(s) 1260
Asp700I GAANNNNTTC 4 cut(s) 126, 446, 747, 1024
AspLEI GCGC 1 cut(s) 1668
AspS9I GGNCC 9 cut(s) 16, 31, 996, 1559, 1789, 1871, 2138, 2731, 3411
AsuHPI GGTGA 6 cut(s) 118, 724, 1377, 1452, 2453, 2528
AvaI CYCGRG 1 cut(s) 1487
AvaII GGWCC 5 cut(s) 996, 1559, 1871, 2138, 3411
BalI TGGCCA 2 cut(s) 11, 2556
BanI GGYRCC 3 cut(s) 92, 141, 2643
BanII GRGCYC 1 cut(s) 137
BauI CACGAG 2 cut(s) 1992, 2894
BbsI GAAGAC 3 cut(s) 344, 1756, 2580
Bbv12I GWGCWC 2 cut(s) 2001, 2628
BbvI GCAGC 9 cut(s) 145, 547, 1152, 2196, 2201, 2243, 2715, 3475, 3556
BceAI ACGGC 1 cut(s) 1178
BciT130I CCWGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
BciVI GTATCC 1 cut(s) 3292
BclI TGATCA 2 cut(s) 415, 910
BcoDI GTCTC 5 cut(s) 58, 607, 2237, 2310, 3427
BfmI CTRYAG 5 cut(s) 943, 1818, 2257, 3263, 3489
BfrI CTTAAG 1 cut(s) 724
BfuAI ACCTGC 3 cut(s) 1755, 1762, 2281
BfuI GTATCC 1 cut(s) 3292
BglII AGATCT 3 cut(s) 444, 2935, 3330
BmcAI AGTACT 1 cut(s) 1148
Bme1390I CCNGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
Bme18I GGWCC 5 cut(s) 996, 1559, 1871, 2138, 3411
BmeT110I CYCGRG 1 cut(s) 1487
BmgBI CACGTC 1 cut(s) 542
BmgT120I GGNCC 9 cut(s) 16, 31, 996, 1559, 1789, 1871, 2138, 2731, 3411
BmiI GGNNCC 7 cut(s) 94, 143, 1560, 1561, 2645, 2919, 3412
BmrFI CCNGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
BmrI ACTGGG 3 cut(s) 520, 2649, 3195
BmuI ACTGGG 3 cut(s) 520, 2649, 3195
BoxI GACNNNNGTC 1 cut(s) 2463
BpiI GAAGAC 3 cut(s) 344, 1756, 2580
BpmI CTGGAG 1 cut(s) 3321
Bpu10I CCTNAGC 1 cut(s) 2000
BpuEI CTTGAG 4 cut(s) 581, 1339, 1341, 1506
BsaAI YACGTR 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 2310
BsaJI CCNNGG 8 cut(s) 12, 145, 146, 735, 1129, 1488, 2102, 3472
BsaWI WCCGGW 2 cut(s) 454, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 686, 716
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 1556, 2026
Bse118I RCCGGY 2 cut(s) 33, 1260
Bse1I ACTGG 6 cut(s) 484, 515, 2644, 3201, 3414, 3444
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
BseDI CCNNGG 8 cut(s) 12, 145, 146, 735, 1129, 1488, 2102, 3472
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 461, 1556, 2026
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 6 cut(s) 518, 1382, 1653, 2014, 2639, 3246
BseNI ACTGG 6 cut(s) 484, 515, 2644, 3201, 3414, 3444
BseRI GAGGAG 2 cut(s) 124, 707
BseXI GCAGC 9 cut(s) 145, 547, 1152, 2196, 2201, 2243, 2715, 3475, 3556
BseYI CCCAGC 1 cut(s) 950
BsgI GTGCAG 2 cut(s) 1330, 2203
Bsh1236I CGCG 2 cut(s) 303, 737
BshFI GGCC 8 cut(s) 11, 17, 33, 316, 1790, 2556, 2733, 3074
BshNI GGYRCC 3 cut(s) 92, 141, 2643
BshTI ACCGGT 1 cut(s) 1260
BsiHKAI GWGCWC 2 cut(s) 2001, 2628
BsiHKCI CYCGRG 1 cut(s) 1487
BsiSI CCGG 3 cut(s) 34, 455, 1261
BslFI GGGAC 6 cut(s) 163, 559, 1173, 1572, 3182, 3489
BslI CCNNNNNNNGG 3 cut(s) 461, 1556, 2026
BsmAI GTCTC 5 cut(s) 58, 607, 2237, 2310, 3427
BsmFI GGGAC 6 cut(s) 163, 559, 1173, 1572, 3182, 3489
BsmI GAATGC 2 cut(s) 1766, 1895
BsnI GGCC 8 cut(s) 11, 17, 33, 316, 1790, 2556, 2733, 3074
Bso31I GGTCTC 1 cut(s) 2310
BsoBI CYCGRG 1 cut(s) 1487
Bsp1286I GDGCHC 3 cut(s) 137, 2001, 2628
Bsp13I TCCGGA 1 cut(s) 454
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 9 cut(s) 5, 303, 323, 735, 737, 1588, 2059, 2613, 2765
BspANI GGCC 8 cut(s) 11, 17, 33, 316, 1790, 2556, 2733, 3074
BspCNI CTCAG 6 cut(s) 517, 1381, 1652, 2013, 2640, 3247
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 2 cut(s) 303, 737
BspHI TCATGA 2 cut(s) 418, 913
BspLI GGNNCC 7 cut(s) 94, 143, 1560, 1561, 2645, 2919, 3412
BspMAI CTGCAG 2 cut(s) 2261, 3493
BspMI ACCTGC 3 cut(s) 1755, 1762, 2281
BspPI GGATC 5 cut(s) 1908, 2183, 2576, 3062, 3282
BspT107I GGYRCC 3 cut(s) 92, 141, 2643
BspTI CTTAAG 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 2310
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 2 cut(s) 33, 1260
BsrI ACTGG 6 cut(s) 484, 515, 2644, 3201, 3414, 3444
BssAI RCCGGY 2 cut(s) 33, 1260
BssECI CCNNGG 8 cut(s) 12, 145, 146, 735, 1129, 1488, 2102, 3472
BssNAI GTATAC 1 cut(s) 1693
BssSI CACGAG 2 cut(s) 1992, 2894
BssT1I CCWWGG 2 cut(s) 12, 2102
Bst1107I GTATAC 1 cut(s) 1693
Bst2BI CACGAG 2 cut(s) 1992, 2894
Bst2UI CCWGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
Bst6I CTCTTC 1 cut(s) 2342
BstAFI CTTAAG 1 cut(s) 724
BstAPI GCANNNNNTGC 1 cut(s) 2190
BstBAI YACGTR 1 cut(s) 206
BstDSI CCRYGG 2 cut(s) 12, 735
BstFNI CGCG 2 cut(s) 303, 737
BstHHI GCGC 1 cut(s) 1668
BstMAI GTCTC 5 cut(s) 58, 607, 2237, 2310, 3427
BstMWI GCNNNNNNNGC 6 cut(s) 322, 956, 1532, 2190, 2619, 3544
BstNI CCWGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
BstNSI RCATGY 4 cut(s) 165, 1106, 2613, 3510
BstPAI GACNNNNGTC 1 cut(s) 2463
BstSCI CCNGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
BstSFI CTRYAG 5 cut(s) 943, 1818, 2257, 3263, 3489
BstUI CGCG 2 cut(s) 303, 737
BstV1I GCAGC 9 cut(s) 145, 547, 1152, 2196, 2201, 2243, 2715, 3475, 3556
BstV2I GAAGAC 3 cut(s) 344, 1756, 2580
BstX2I RGATCY 5 cut(s) 444, 2581, 2935, 3054, 3330
BstXI CCANNNNNNTGG 1 cut(s) 3013
BstYI RGATCY 5 cut(s) 444, 2581, 2935, 3054, 3330
BstZ17I GTATAC 1 cut(s) 1693
BsuI GTATCC 1 cut(s) 3292
BsuRI GGCC 8 cut(s) 11, 17, 33, 316, 1790, 2556, 2733, 3074
BtgI CCRYGG 2 cut(s) 12, 735
BtgZI GCGATG 1 cut(s) 284
BtrI CACGTC 1 cut(s) 542
BtsI GCAGTG 2 cut(s) 668, 1054
BtsIMutI CAGTG 9 cut(s) 668, 1054, 1376, 1404, 1873, 2061, 2637, 3036, 3208
BveI ACCTGC 3 cut(s) 1755, 1762, 2281
CaiI CAGNNNCTG 2 cut(s) 3254, 3271
CciI TCATGA 2 cut(s) 418, 913
CfoI GCGC 1 cut(s) 1668
Cfr10I RCCGGY 2 cut(s) 33, 1260
Cfr13I GGNCC 9 cut(s) 16, 31, 996, 1559, 1789, 1871, 2138, 2731, 3411
Cfr42I CCGCGG 1 cut(s) 738
CseI GACGC 1 cut(s) 1446
Csp6I GTAC 8 cut(s) 592, 872, 1147, 1264, 2114, 2421, 3080, 3541
CspAI ACCGGT 1 cut(s) 1260
CviQI GTAC 8 cut(s) 592, 872, 1147, 1264, 2114, 2421, 3080, 3541
DraIII CACNNNGTG 1 cut(s) 2643
EaeI YGGCCR 3 cut(s) 9, 2554, 3072
Eam1104I CTCTTC 1 cut(s) 2342
EarI CTCTTC 1 cut(s) 2342
Eco130I CCWWGG 2 cut(s) 12, 2102
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 2310
Eco32I GATATC 3 cut(s) 683, 2724, 2950
Eco47I GGWCC 5 cut(s) 996, 1559, 1871, 2138, 3411
Eco57I CTGAAG 5 cut(s) 1155, 1428, 2816, 2941, 3447
Eco88I CYCGRG 1 cut(s) 1487
EcoO109I RGGNCCY 2 cut(s) 996, 1559
EcoRI GAATTC 1 cut(s) 835
EcoRII CCWGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
EcoRV GATATC 3 cut(s) 683, 2724, 2950
EcoT14I CCWWGG 2 cut(s) 12, 2102
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 2 cut(s) 12, 2102
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 3069, 3101
FaqI GGGAC 6 cut(s) 163, 559, 1173, 1572, 3182, 3489
FauI CCCGC 2 cut(s) 2066, 2758
FauNDI CATATG 2 cut(s) 1537, 2417
FbaI TGATCA 2 cut(s) 415, 910
FblI GTMKAC 2 cut(s) 1692, 3112
FriOI GRGCYC 1 cut(s) 137
GlaI GCGC 1 cut(s) 1667
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 1 cut(s) 3321
HaeIII GGCC 8 cut(s) 11, 17, 33, 316, 1790, 2556, 2733, 3074
HapII CCGG 3 cut(s) 34, 455, 1261
HgaI GACGC 1 cut(s) 1446
HhaI GCGC 1 cut(s) 1668
Hin6I GCGC 1 cut(s) 1666
HinP1I GCGC 1 cut(s) 1666
HincII GTYRAC 2 cut(s) 3113, 3437
HindII GTYRAC 2 cut(s) 3113, 3437
HindIII AAGCTT 1 cut(s) 2741
HpaII CCGG 3 cut(s) 34, 455, 1261
HphI GGTGA 6 cut(s) 118, 724, 1377, 1452, 2453, 2528
Hpy166II GTNNAC 7 cut(s) 666, 1385, 1693, 1871, 2205, 3113, 3437
Hpy8I GTNNAC 7 cut(s) 666, 1385, 1693, 1871, 2205, 3113, 3437
Hpy99I CGWCG 2 cut(s) 543, 3114
HpyCH4IV ACGT 2 cut(s) 205, 541
HpyF10VI GCNNNNNNNGC 6 cut(s) 322, 956, 1532, 2190, 2619, 3544
HpySE526I ACGT 2 cut(s) 205, 541
HspAI GCGC 1 cut(s) 1666
KflI GGGWCCC 1 cut(s) 1559
Kpn2I TCCGGA 1 cut(s) 454
KroI GCCGGC 1 cut(s) 33
KroNI GCCGGC 1 cut(s) 35
Ksp22I TGATCA 2 cut(s) 415, 910
KspI CCGCGG 1 cut(s) 738
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 9 cut(s) 145, 547, 1152, 2196, 2201, 2243, 2715, 3475, 3556
MaeII ACGT 2 cut(s) 205, 541
MflI RGATCY 5 cut(s) 444, 2581, 2935, 3054, 3330
MhlI GDGCHC 3 cut(s) 137, 2001, 2628
MlsI TGGCCA 2 cut(s) 11, 2556
MluNI TGGCCA 2 cut(s) 11, 2556
MlyI GAGTC 8 cut(s) 224, 357, 812, 1130, 2025, 2233, 2239, 3260
MmeI TCCRAC 3 cut(s) 684, 1199, 2652
Mox20I TGGCCA 2 cut(s) 11, 2556
Mph1103I ATGCAT 1 cut(s) 394
MroI TCCGGA 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 33
MroXI GAANNNNTTC 4 cut(s) 126, 446, 747, 1024
MscI TGGCCA 2 cut(s) 11, 2556
MslI CAYNNNNRTG 4 cut(s) 387, 423, 1369, 2436
Msp20I TGGCCA 2 cut(s) 11, 2556
MspA1I CMGCKG 5 cut(s) 737, 1190, 2187, 2765, 3341
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 3 cut(s) 34, 455, 1261
MspR9I CCNGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
Mva1269I GAATGC 2 cut(s) 1766, 1895
MvaI CCWGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
MvnI CGCG 2 cut(s) 303, 737
MwoI GCNNNNNNNGC 6 cut(s) 322, 956, 1532, 2190, 2619, 3544
NaeI GCCGGC 1 cut(s) 35
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 2 cut(s) 1537, 2417
NgoMIV GCCGGC 1 cut(s) 33
NlaIV GGNNCC 7 cut(s) 94, 143, 1560, 1561, 2645, 2919, 3412
NmuCI GTSAC 6 cut(s) 1402, 1458, 2429, 2456, 2605, 2787
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 4 cut(s) 165, 1106, 2613, 3510
OliI CACNNNNGTG 2 cut(s) 1369, 2436
PaeI GCATGC 2 cut(s) 165, 3510
PagI TCATGA 2 cut(s) 418, 913
PasI CCCWGGG 1 cut(s) 146
PciI ACATGT 1 cut(s) 1102
PctI GAATGC 2 cut(s) 1766, 1895
PdiI GCCGGC 1 cut(s) 35
PdmI GAANNNNTTC 4 cut(s) 126, 446, 747, 1024
PfeI GAWTC 8 cut(s) 269, 786, 1283, 1337, 1423, 1544, 2053, 2681
PflFI GACNNNGTC 1 cut(s) 3250
PflMI CCANNNNNTGG 1 cut(s) 2026
PfoI TCCNGGA 2 cut(s) 2019, 3285
PinAI ACCGGT 1 cut(s) 1260
PleI GAGTC 8 cut(s) 223, 357, 811, 1129, 2024, 2232, 2239, 3259
PpsI GAGTC 8 cut(s) 223, 357, 811, 1129, 2024, 2232, 2239, 3259
Ppu21I YACGTR 1 cut(s) 206
PpuMI RGGWCCY 2 cut(s) 996, 1559
PscI ACATGT 1 cut(s) 1102
PshAI GACNNNNGTC 1 cut(s) 2463
Psp5II RGGWCCY 2 cut(s) 996, 1559
Psp6I CCWGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
PspN4I GGNNCC 7 cut(s) 94, 143, 1560, 1561, 2645, 2919, 3412
PspPI GGNCC 9 cut(s) 16, 31, 996, 1559, 1789, 1871, 2138, 2731, 3411
PspPPI RGGWCCY 2 cut(s) 996, 1559
PstI CTGCAG 2 cut(s) 2261, 3493
PstNI CAGNNNCTG 2 cut(s) 3254, 3271
PsuI RGATCY 5 cut(s) 444, 2581, 2935, 3054, 3330
PsyI GACNNNGTC 1 cut(s) 3250
PvuII CAGCTG 3 cut(s) 1190, 2187, 3341
RsaI GTAC 8 cut(s) 593, 873, 1148, 1265, 2115, 2422, 3081, 3542
RsaNI GTAC 8 cut(s) 592, 872, 1147, 1264, 2114, 2421, 3080, 3541
RseI CAYNNNNRTG 4 cut(s) 387, 423, 1369, 2436
SacII CCGCGG 1 cut(s) 738
SalI GTCGAC 1 cut(s) 3111
Sau96I GGNCC 9 cut(s) 16, 31, 996, 1559, 1789, 1871, 2138, 2731, 3411
ScaI AGTACT 1 cut(s) 1148
SchI GAGTC 8 cut(s) 224, 357, 812, 1130, 2025, 2233, 2239, 3260
ScrFI CCNGG 8 cut(s) 147, 791, 1131, 1389, 2021, 2929, 3287, 3473
SduI GDGCHC 3 cut(s) 137, 2001, 2628
SfcI CTRYAG 5 cut(s) 943, 1818, 2257, 3263, 3489
Sfr303I CCGCGG 1 cut(s) 738
SgrBI CCGCGG 1 cut(s) 738
SinI GGWCC 5 cut(s) 996, 1559, 1871, 2138, 3411
SmiMI CAYNNNNRTG 4 cut(s) 387, 423, 1369, 2436
SmlI CTYRAG 5 cut(s) 560, 724, 1318, 1356, 1521
SmoI CTYRAG 5 cut(s) 560, 724, 1318, 1356, 1521
SphI GCATGC 2 cut(s) 165, 3510
SsiI CCGC 9 cut(s) 5, 303, 323, 735, 737, 1588, 2059, 2613, 2765
SspI AATATT 5 cut(s) 1514, 1812, 2284, 3376, 3397
StyD4I CCNGG 8 cut(s) 145, 789, 1129, 1387, 2019, 2927, 3285, 3471
StyI CCWWGG 2 cut(s) 12, 2102
TaiI ACGT 2 cut(s) 208, 544
TaqI TCGA 6 cut(s) 631, 983, 1331, 2337, 2398, 3112
TatI WGTACW 4 cut(s) 1146, 2113, 2420, 3540
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 8 cut(s) 269, 786, 1283, 1337, 1423, 1544, 2053, 2681
TscAI CASTG 9 cut(s) 668, 1054, 1376, 1411, 1873, 2068, 2644, 3043, 3208
TseFI GTSAC 6 cut(s) 1402, 1458, 2429, 2456, 2605, 2787
TseI GCWGC 9 cut(s) 158, 535, 1165, 2184, 2214, 2256, 2703, 3488, 3569
Tsp45I GTSAC 6 cut(s) 1402, 1458, 2429, 2456, 2605, 2787
TspGWI ACGGA 2 cut(s) 2867, 3075
TspRI CASTG 9 cut(s) 668, 1054, 1376, 1411, 1873, 2068, 2644, 3043, 3208
Tth111I GACNNNGTC 1 cut(s) 3250
Van91I CCANNNNNTGG 1 cut(s) 2026
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 5 cut(s) 996, 1559, 1871, 2138, 3411
XapI RAATTY 6 cut(s) 835, 1056, 1738, 1848, 3139, 3562
XceI RCATGY 4 cut(s) 165, 1106, 2613, 3510
XcmI CCANNNNNNNNNTGG 2 cut(s) 2287, 2517
XmiI GTMKAC 2 cut(s) 1692, 3112
XmnI GAANNNNTTC 4 cut(s) 126, 446, 747, 1024
ZrmI AGTACT 1 cut(s) 1148
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.