RchiOBHm_Chr6g0252611

ubiquitinyl hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001222
Physical Location & Seq
Reverse (-)
7762284 .. 7768375
6092 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22652

Sequence Viewer

Length: 2889 bp
ATGGAAGGGTTTTGGGGTGGTGAAAGGAAGAATCACAGAGTGAAGCATCTTGCATTCAAACAGAGGAGTAGAGGTAGTGTTAGCATGTCATCCTTGAGCAAATTTGTGGAGTTTGACAATGCAAATGGTGAAAAGGAAGAGAGTGCTACCACTCCGAAGTTGAAAAGGCGAAAGCCGTCTGCATCTTCCTGTGGTTATAGAACTGATAGTGATGGTAGTCAGCAAACTCGTGTGCTGAGGTCAAGAAATCGAGTGCAGGAAGTGGTTACTCCTACTTCCACACACCATCATCCTTGTACAGTCTTATCTTGGCTGATTGACAACAATGTGGTTTTGCCAAGGGAGAAATTGCGTTATTGTAGCGCAAGGGGGAGTCATGCTGTGGCAGAAGGAAAAGTATGTAGAGAGGGGATTGAATGCAACTGCTGCCAGCAGGTTTTTACACTTGGTCGCTTTGACAGTCATGCTGGCTGCAGTAACTACAGACCTGCAGCCAATATTTTTCTGGAAGATGGAAAGTCTCTCCTAGATTGCCAGATGCAAATTATGACTGAAAGAGGGAAGAAGAGATGCAGACTAGAACCACATGATGTGATGAAGGGTAATTTGTCTCGAATAGAAAATGATAGCTTTTGTACAGTCTGTCAGGATGGTGGTGATTTGATATTATGTGATCGATGTCCATCAGCATTTCACAAGAGTTGCATTGGTTTCGAGGATGTTCCAGATGGTGACTGGTTCTGTCCAGCTTGCTGTTGTGTAATTTGTGGCTGCAGCATAAATCCTACAATCCATTACCATACCTGTGGTCAATGTGAACGTAAATATCACACCAGCTGCCTGAGGAAAAGAAGCATAGATAATTTAGAGATGAATTCAAAAGGAAATTGGTTCTGCAGCAACGACTGTAAAAAGATATTTTTAGGCCTCCACGAGCGTTTAGGGAAACAAATTTCAGTGGGTGTTGACAATCTAACTTGGTCACTTTGGAAGTCCGTGAAATCTGATAGTGATAATCCTAATGAACCTGATAATGATGCTGCAACGGAGACTTATACCAAACTCAATCTTGCCCTTCAAGTGATGCAGGAGTGTTTTAAGCATTTTAAGAAACCTCACACTGGGGGATATCTTGCAGAAGATATCATCTTCAGCAGAGGGTCACATCTTGATGGCATGAACTTTCGGGGGTTTTACACTCTGATTTTGGAGAAAAATGACGCTTTAATAACTGTAGCTACCGTAAGGATCTATGGAGCAAAGGTGGCTGAAGTACCTCTTATTGGTACCAGATTGCAGCATCGTCAAAGTGGAATGTGTCGTGTATTGATGCATTTAATTGAAAAGATGCTTATGGACTTGGGTGTGGAGAGATTGGTTTTGCCCATTGTCCCCAGATCAGTAGGAAAATGGAATACTTCATTTGGATTTTCAGATATTAAAGGTTCTGATAGGCTGCAGTTTCTGGATTATAGATTCATGCATTTTGAGGATACCATTATGTGTCAGAAATGCTTAAAAAGGACATATCTAGCTGAACCATGCCCATCAAAAGGAATTTCGCTTGATGTTGCTGGAAGTGTTGATGATATTGATTTTGATGGGTCCAGCGTCCATAAAGTCTCATTCACATGGAAAATTTACAATTTCTCTAGCCGTGGGAAGGAATACTCTGAGACTTTCCTCACTGGTGATTTGAGATGGCGGATGCTTATGTATCCAAAGGGCAACACTGTTGATAACTTGTCAGTATATTTGGATGTTCGAGGTGCCTCAGAATTGCCACTTGGGTGGTCTAGATGTGTGAAATTCAGCTTGACTGTGGTTAATCAACTTCAACACAGCCAGTCAATAACAAGGGAAACTAAACATATGTTCAGTAAGAAAGTTGGTAACTGTGGCTTCACATCACTAATTCCTCTAGATGAGTTACATGACCATTGTAAAGGGTTTCTTGTGAATGATACATGTATCATTATAGCCAATGTTGCAGTCTGCATGGCTCAAATTATGATATCAAAAGACCTAAAAATTGGCAGCGCTACATTGAGACCTGTGGAGTACTTAAATCAGCAGCAGGGGCCTCCTTTACATAAAGGTTCCGTGAAAATTTCAGATCCTTCTCCAGCAGCGCACACGACCACAGGCTTTGAACATGCAACTCTCTTCCAGGACACAGCAAGTTCTGAACAAGCTTGCACCAATATTCCTGATAGTAGGACCTACCCTTCTTTAAAATTGGAGTCTGTGGAAGTTCCAGATATTTTTGCAGCAGCATGCAAGGCAACTAGCTTTGGACACGTGATTGCCACCCAGGATGCAGCAAGTTTTGAACAAGTCTGCATCAAGCTCTCTGATACCAGTACTGTCGACCCTTCCCTCAAGAAATTTGATGTTGTGTCCTCCACATCAACTTCTGAACTCATGAACTTCAGGGGCTTGGCGAATATAAAGAAAGATTTTGTTCCCCTGCTGGAGGAAGTGTGTTCCAGGCATCCTTCTTTCATTGAATGCCAAAAGGAGAGTAGTCCCCAGTTTACTGAATGGGCATTCACTGCCTTGGGAAAAGTTCTTCATTTTTTGAAGACTAAAAAGGTCAAGGATATGACAACATCTGTCTGTGAAGATCTAGACCTTTTATACAAGGAGCTGAAGGCCTTCAAATTTGATTTGACTTGGCTAGAGCCTCATGTTAAAACAGCTCTGGGTGGGAGTGAGTATGTGGAAAGGGCAGCAGAGGTAAAGAGACTAAGAGAGAATGTGAAGGCTTTGGAGATTGACTCAAAGAGCCTTGAGGCTAAGCTTGTGGCAGCAAGAAATGATCTGAAAAAGGCTGAAGAATCCTTTGATGAGAGAATTATGGACAGCGAGTTAGGTTATTCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko05168 ko05169 ko05203 map04068 map05168 map05169 map05203
Pfam Domains
Protein Families
MATH UCH USP7_C2 USP7_ICP0_bdg

Protein Analysis

962

Amino Acids

108.09

Weight (kDa)

7.97

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 132 - 181 2.6e-18 Tify domain binding domain
PHD-1st_NSD PF23011 209 - 251 6.6e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 212 - 251 2e-08 PHD-finger
IDM1_C PF23209 366 - 504 3.9e-46 Increased DNA methylation 1, C-terminal domain
MATH_2 PF22486 542 - 663 1.9e-28 MATH domain
MATH PF00917 549 - 664 1.7e-12 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 424, 496
Acc65I GGTACC 1 cut(s) 1286
AccB1I GGYRCC 2 cut(s) 1286, 1769
AccI GTMKAC 1 cut(s) 2370
AciI CCGC 1 cut(s) 1705
AclWI GGATC 2 cut(s) 1256, 2111
AcsI RAATTY 9 cut(s) 101, 874, 951, 1557, 1638, 1808, 2109, 2387, 2663
AcuI CTGAAG 5 cut(s) 1135, 1290, 2416, 2672, 2856
AcvI CACGTG 1 cut(s) 2302
AdeI CACNNNGTG 1 cut(s) 40
AfaI GTAC 6 cut(s) 298, 637, 1275, 1288, 2063, 2365
AfeI AGCGCT 1 cut(s) 2041
AfiI CCNNNNNNNGG 5 cut(s) 1121, 1283, 1553, 1663, 2476
AflIII ACRYGT 2 cut(s) 1967, 2299
AjnI CCWGG 3 cut(s) 2169, 2313, 2489
AjuI GAANNNNNNNTTGG 2 cut(s) 1770, 1802
AleI CACNNNNGTG 1 cut(s) 1789
Alw26I GTCTC 7 cut(s) 525, 615, 1043, 1627, 1670, 2044, 2740
AlwI GGATC 2 cut(s) 1256, 2111
AlwNI CAGNNNCTG 1 cut(s) 1465
Aor51HI AGCGCT 1 cut(s) 2041
AoxI GGCC 3 cut(s) 925, 2081, 2655
ApoI RAATTY 9 cut(s) 101, 874, 951, 1557, 1638, 1808, 2109, 2387, 2663
Asp700I GAANNNNTTC 1 cut(s) 2657
Asp718I GGTACC 1 cut(s) 1286
AspLEI GCGC 3 cut(s) 365, 2042, 2134
AspS9I GGNCC 3 cut(s) 1605, 2081, 2220
AsuHPI GGTGA 5 cut(s) 32, 140, 668, 743, 1703
AvaII GGWCC 2 cut(s) 1605, 2220
AxyI CCTNAGG 1 cut(s) 842
BanI GGYRCC 2 cut(s) 1286, 1769
BauI CACGAG 2 cut(s) 228, 932
BbrPI CACGTG 1 cut(s) 2302
BbsI GAAGAC 1 cut(s) 2591
BbvCI CCTCAGC 1 cut(s) 236
BceAI ACGGC 2 cut(s) 160, 1641
BcgI CGANNNNNNTGC 2 cut(s) 694, 728
BciT130I CCWGG 3 cut(s) 2171, 2315, 2491
BciVI GTATCC 2 cut(s) 1486, 1728
BcoDI GTCTC 7 cut(s) 525, 615, 1043, 1627, 1670, 2044, 2740
BfaI CTAG 9 cut(s) 527, 578, 1532, 1653, 1797, 1922, 2289, 2630, 2681
BfmI CTRYAG 7 cut(s) 472, 481, 489, 772, 895, 1233, 1457
BfoI RGCGCY 1 cut(s) 2043
BfuAI ACCTGC 2 cut(s) 424, 496
BfuI GTATCC 2 cut(s) 1486, 1728
BglII AGATCT 1 cut(s) 2626
BlpI GCTNAGC 1 cut(s) 2799
BmcAI AGTACT 2 cut(s) 2063, 2365
Bme1390I CCNGG 3 cut(s) 2171, 2315, 2491
Bme18I GGWCC 2 cut(s) 1605, 2220
BmgT120I GGNCC 3 cut(s) 1605, 2081, 2220
BmiI GGNNCC 5 cut(s) 1288, 1606, 1771, 2082, 2101
BmrFI CCNGG 3 cut(s) 2171, 2315, 2491
BmrI ACTGGG 2 cut(s) 1131, 2527
BmuI ACTGGG 2 cut(s) 1131, 2527
BpiI GAAGAC 1 cut(s) 2591
BplI GAGNNNNNCTC 2 cut(s) 2765, 2797
BpmI CTGGAG 2 cut(s) 2109, 2495
Bpu10I CCTNAGC 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 2799
BpuEI CTTGAG 3 cut(s) 115, 2366, 2813
Bsa29I ATCGAT 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 2302
BsaBI GATNNNNATC 1 cut(s) 682
BsaI GGTCTC 1 cut(s) 2044
BsaJI CCNNGG 4 cut(s) 338, 1657, 2313, 2559
BsaXI ACNNNNNCTCC 6 cut(s) 58, 88, 1248, 1278, 2468, 2498
Bsc4I CCNNNNNNNGG 5 cut(s) 1121, 1283, 1553, 1663, 2476
Bse1I ACTGG 6 cut(s) 740, 1126, 1693, 1846, 2361, 2533
Bse21I CCTNAGG 1 cut(s) 842
Bse8I GATNNNNATC 1 cut(s) 682
BseBI CCWGG 3 cut(s) 2171, 2315, 2491
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 4 cut(s) 338, 1657, 2313, 2559
BseGI GGATG 8 cut(s) 89, 289, 655, 724, 1713, 1765, 2323, 2494
BseJI GATNNNNATC 1 cut(s) 682
BseLI CCNNNNNNNGG 5 cut(s) 1121, 1283, 1553, 1663, 2476
BseMII CTCAG 4 cut(s) 227, 833, 1665, 1788
BseNI ACTGG 6 cut(s) 740, 1126, 1693, 1846, 2361, 2533
BseRI GAGGAG 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 3 cut(s) 927, 2083, 2657
BshNI GGYRCC 2 cut(s) 1286, 1769
BshVI ATCGAT 1 cut(s) 676
BslFI GGGAC 2 cut(s) 1376, 2514
BslI CCNNNNNNNGG 5 cut(s) 1121, 1283, 1553, 1663, 2476
BsmAI GTCTC 7 cut(s) 525, 615, 1043, 1627, 1670, 2044, 2740
BsmFI GGGAC 2 cut(s) 1376, 2514
BsmI GAATGC 4 cut(s) 53, 422, 2516, 2549
BsnI GGCC 3 cut(s) 927, 2083, 2657
Bso31I GGTCTC 1 cut(s) 2044
Bsp1407I TGTACA 2 cut(s) 296, 635
Bsp143I GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
Bsp1720I GCTNAGC 1 cut(s) 2799
BspACI CCGC 1 cut(s) 1705
BspANI GGCC 3 cut(s) 927, 2083, 2657
BspCNI CTCAG 4 cut(s) 228, 834, 1666, 1787
BspDI ATCGAT 1 cut(s) 676
BspHI TCATGA 1 cut(s) 2424
BspLI GGNNCC 5 cut(s) 1288, 1606, 1771, 2082, 2101
BspMAI CTGCAG 5 cut(s) 476, 493, 776, 899, 1461
BspMI ACCTGC 2 cut(s) 424, 496
BspPI GGATC 2 cut(s) 1256, 2111
BspT107I GGYRCC 2 cut(s) 1286, 1769
BspTNI GGTCTC 1 cut(s) 2044
BsrGI TGTACA 2 cut(s) 296, 635
BsrI ACTGG 6 cut(s) 740, 1126, 1693, 1846, 2361, 2533
BssECI CCNNGG 4 cut(s) 338, 1657, 2313, 2559
BssMI GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
BssSI CACGAG 2 cut(s) 228, 932
BssT1I CCWWGG 2 cut(s) 338, 2559
Bst2BI CACGAG 2 cut(s) 228, 932
Bst2UI CCWGG 3 cut(s) 2171, 2315, 2491
Bst6I CTCTTC 3 cut(s) 132, 560, 2171
BstAPI GCANNNNNTGC 2 cut(s) 426, 2555
BstAUI TGTACA 2 cut(s) 296, 635
BstBAI YACGTR 1 cut(s) 2302
BstC8I GCNNGC 5 cut(s) 431, 469, 751, 2197, 2278
BstDEI CTNAG 6 cut(s) 236, 842, 1674, 1774, 2750, 2799
BstDSI CCRYGG 1 cut(s) 1657
BstENI CCTNNNNNAGG 1 cut(s) 2474
BstF5I GGATG 8 cut(s) 89, 289, 655, 724, 1713, 1765, 2323, 2494
BstH2I RGCGCY 1 cut(s) 2043
BstHHI GCGC 3 cut(s) 365, 2042, 2134
BstKTI GATC 6 cut(s) 676, 1251, 1400, 2119, 2629, 2824
BstMAI GTCTC 7 cut(s) 525, 615, 1043, 1627, 1670, 2044, 2740
BstMBI GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
BstMWI GCNNNNNNNGC 6 cut(s) 426, 1265, 1988, 2080, 2282, 2555
BstNI CCWGG 3 cut(s) 2171, 2315, 2491
BstNSI RCATGY 4 cut(s) 88, 1971, 2159, 2280
BstSCI CCNGG 3 cut(s) 2169, 2313, 2489
BstSFI CTRYAG 7 cut(s) 472, 481, 489, 772, 895, 1233, 1457
BstV2I GAAGAC 1 cut(s) 2591
BstX2I RGATCY 3 cut(s) 1248, 2116, 2626
BstXI CCANNNNNNTGG 2 cut(s) 806, 1791
BstYI RGATCY 3 cut(s) 1248, 2116, 2626
Bsu15I ATCGAT 1 cut(s) 676
Bsu36I CCTNAGG 1 cut(s) 842
BsuI GTATCC 2 cut(s) 1486, 1728
BsuRI GGCC 3 cut(s) 927, 2083, 2657
BsuTUI ATCGAT 1 cut(s) 676
BtgI CCRYGG 1 cut(s) 1657
BtsCI GGATG 8 cut(s) 89, 289, 655, 724, 1713, 1765, 2323, 2494
BtsI GCAGTG 1 cut(s) 2553
BtsIMutI CAGTG 5 cut(s) 963, 1119, 1686, 1731, 2553
BveI ACCTGC 2 cut(s) 424, 496
Cac8I GCNNGC 5 cut(s) 431, 469, 751, 2197, 2278
CaiI CAGNNNCTG 1 cut(s) 1465
CciI TCATGA 1 cut(s) 2424
CfoI GCGC 3 cut(s) 365, 2042, 2134
Cfr13I GGNCC 3 cut(s) 1605, 2081, 2220
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 2 cut(s) 1229, 1600
Csp6I GTAC 6 cut(s) 297, 636, 1274, 1287, 2062, 2364
CspCI CAANNNNNGTGG 2 cut(s) 2131, 2166
CviQI GTAC 6 cut(s) 297, 636, 1274, 1287, 2062, 2364
DdeI CTNAG 6 cut(s) 236, 842, 1674, 1774, 2750, 2799
DpnI GATC 6 cut(s) 675, 1250, 1399, 2118, 2628, 2823
DpnII GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
DraI TTTAAA 1 cut(s) 2235
DraIII CACNNNGTG 1 cut(s) 40
Eam1104I CTCTTC 3 cut(s) 132, 560, 2171
EarI CTCTTC 3 cut(s) 132, 560, 2171
EciI GGCGGA 1 cut(s) 1720
Eco130I CCWWGG 2 cut(s) 338, 2559
Eco147I AGGCCT 2 cut(s) 927, 2657
Eco31I GGTCTC 1 cut(s) 2044
Eco32I GATATC 3 cut(s) 1130, 1144, 2016
Eco47I GGWCC 2 cut(s) 1605, 2220
Eco47III AGCGCT 1 cut(s) 2041
Eco57I CTGAAG 5 cut(s) 1135, 1290, 2416, 2672, 2856
Eco72I CACGTG 1 cut(s) 2302
Eco81I CCTNAGG 1 cut(s) 842
EcoNI CCTNNNNNAGG 1 cut(s) 2474
EcoO109I RGGNCCY 2 cut(s) 2081, 2220
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 3 cut(s) 2169, 2313, 2489
EcoRV GATATC 3 cut(s) 1130, 1144, 2016
EcoT14I CCWWGG 2 cut(s) 338, 2559
EcoT22I ATGCAT 2 cut(s) 1335, 1485
ErhI CCWWGG 2 cut(s) 338, 2559
FalI AAGNNNNNCTT 4 cut(s) 1263, 1295, 1938, 1970
FaqI GGGAC 2 cut(s) 1376, 2514
FauNDI CATATG 1 cut(s) 1872
FblI GTMKAC 1 cut(s) 2370
FokI GGATG 8 cut(s) 76, 276, 662, 731, 1720, 1772, 2330, 2481
FspBI CTAG 9 cut(s) 527, 578, 1532, 1653, 1797, 1922, 2289, 2630, 2681
GlaI GCGC 3 cut(s) 364, 2041, 2133
GsuI CTGGAG 2 cut(s) 2109, 2495
HaeII RGCGCY 1 cut(s) 2043
HaeIII GGCC 3 cut(s) 927, 2083, 2657
HgaI GACGC 2 cut(s) 1229, 1600
HhaI GCGC 3 cut(s) 365, 2042, 2134
Hin6I GCGC 3 cut(s) 363, 2040, 2132
HinP1I GCGC 3 cut(s) 363, 2040, 2132
HincII GTYRAC 2 cut(s) 967, 2371
HindII GTYRAC 2 cut(s) 967, 2371
HindIII AAGCTT 2 cut(s) 2193, 2801
HinfI GANTC 6 cut(s) 31, 373, 1476, 2243, 2780, 2840
HphI GGTGA 5 cut(s) 32, 140, 668, 743, 1703
Hpy166II GTNNAC 4 cut(s) 818, 967, 2371, 2538
Hpy8I GTNNAC 4 cut(s) 818, 967, 2371, 2538
HpyCH4IV ACGT 2 cut(s) 820, 2301
HpyF10VI GCNNNNNNNGC 6 cut(s) 426, 1265, 1988, 2080, 2282, 2555
HpyF3I CTNAG 6 cut(s) 236, 842, 1674, 1774, 2750, 2799
HpySE526I ACGT 2 cut(s) 820, 2301
HspAI GCGC 3 cut(s) 363, 2040, 2132
KpnI GGTACC 1 cut(s) 1290
Kzo9I GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
LmnI GCTCC 2 cut(s) 1256, 2647
MaeI CTAG 9 cut(s) 527, 578, 1532, 1653, 1797, 1922, 2289, 2630, 2681
MaeII ACGT 2 cut(s) 820, 2301
MaeIII GTNAC 7 cut(s) 265, 476, 731, 981, 1161, 1892, 1929
MalI GATC 6 cut(s) 675, 1250, 1399, 2118, 2628, 2823
MboI GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
MflI RGATCY 3 cut(s) 1248, 2116, 2626
MlyI GAGTC 3 cut(s) 382, 2252, 2774
Mph1103I ATGCAT 2 cut(s) 1335, 1485
MroXI GAANNNNTTC 1 cut(s) 2657
MslI CAYNNNNRTG 4 cut(s) 804, 1170, 1630, 1789
MspA1I CMGCKG 1 cut(s) 837
MspR9I CCNGG 3 cut(s) 2171, 2315, 2491
Mva1269I GAATGC 4 cut(s) 53, 422, 2516, 2549
MvaI CCWGG 3 cut(s) 2171, 2315, 2491
MwoI GCNNNNNNNGC 6 cut(s) 426, 1265, 1988, 2080, 2282, 2555
NdeI CATATG 1 cut(s) 1872
NdeII GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
NlaIV GGNNCC 5 cut(s) 1288, 1606, 1771, 2082, 2101
NmuCI GTSAC 3 cut(s) 731, 981, 1161
NsiI ATGCAT 2 cut(s) 1335, 1485
NspI RCATGY 4 cut(s) 88, 1971, 2159, 2280
OliI CACNNNNGTG 1 cut(s) 1789
PaeI GCATGC 1 cut(s) 2280
PagI TCATGA 1 cut(s) 2424
PceI AGGCCT 2 cut(s) 927, 2657
PciI ACATGT 1 cut(s) 1967
PctI GAATGC 4 cut(s) 53, 422, 2516, 2549
PdmI GAANNNNTTC 1 cut(s) 2657
PfeI GAWTC 3 cut(s) 31, 1476, 2840
PfoI TCCNGGA 1 cut(s) 2169
PleI GAGTC 3 cut(s) 381, 2251, 2774
PmaCI CACGTG 1 cut(s) 2302
PmlI CACGTG 1 cut(s) 2302
PpsI GAGTC 3 cut(s) 381, 2251, 2774
Ppu21I YACGTR 1 cut(s) 2302
PpuMI RGGWCCY 1 cut(s) 2220
PscI ACATGT 1 cut(s) 1967
Psp5II RGGWCCY 1 cut(s) 2220
Psp6I CCWGG 3 cut(s) 2169, 2313, 2489
PspCI CACGTG 1 cut(s) 2302
PspGI CCWGG 3 cut(s) 2169, 2313, 2489
PspN4I GGNNCC 5 cut(s) 1288, 1606, 1771, 2082, 2101
PspPI GGNCC 3 cut(s) 1605, 2081, 2220
PspPPI RGGWCCY 1 cut(s) 2220
PstI CTGCAG 5 cut(s) 476, 493, 776, 899, 1461
PstNI CAGNNNCTG 1 cut(s) 1465
PsuI RGATCY 3 cut(s) 1248, 2116, 2626
PvuII CAGCTG 1 cut(s) 837
RsaI GTAC 6 cut(s) 298, 637, 1275, 1288, 2063, 2365
RsaNI GTAC 6 cut(s) 297, 636, 1274, 1287, 2062, 2364
RseI CAYNNNNRTG 4 cut(s) 804, 1170, 1630, 1789
SalI GTCGAC 1 cut(s) 2369
Sau3AI GATC 6 cut(s) 673, 1248, 1397, 2116, 2626, 2821
Sau96I GGNCC 3 cut(s) 1605, 2081, 2220
ScaI AGTACT 2 cut(s) 2063, 2365
SchI GAGTC 3 cut(s) 382, 2252, 2774
ScrFI CCNGG 3 cut(s) 2171, 2315, 2491
SfcI CTRYAG 7 cut(s) 472, 481, 489, 772, 895, 1233, 1457
SinI GGWCC 2 cut(s) 1605, 2220
SmiMI CAYNNNNRTG 4 cut(s) 804, 1170, 1630, 1789
SmlI CTYRAG 3 cut(s) 94, 2381, 2792
SmoI CTYRAG 3 cut(s) 94, 2381, 2792
SphI GCATGC 1 cut(s) 2280
SseBI AGGCCT 2 cut(s) 927, 2657
SsiI CCGC 1 cut(s) 1705
SspI AATATT 2 cut(s) 499, 2206
SspMI CTAG 9 cut(s) 527, 578, 1532, 1653, 1797, 1922, 2289, 2630, 2681
StuI AGGCCT 2 cut(s) 927, 2657
StyD4I CCNGG 3 cut(s) 2169, 2313, 2489
StyI CCWWGG 2 cut(s) 338, 2559
TaiI ACGT 2 cut(s) 823, 2304
TaqI TCGA 6 cut(s) 250, 613, 676, 714, 1765, 2370
TatI WGTACW 4 cut(s) 296, 635, 2061, 2363
TfiI GAWTC 3 cut(s) 31, 1476, 2840
TscAI CASTG 5 cut(s) 963, 1126, 1693, 1738, 2560
TseFI GTSAC 3 cut(s) 731, 981, 1161
Tsp45I GTSAC 3 cut(s) 731, 981, 1161
TspDTI ATGAA 9 cut(s) 611, 887, 1038, 1193, 1410, 1468, 2441, 2494, 2564
TspGWI ACGGA 3 cut(s) 985, 1061, 2092
TspRI CASTG 5 cut(s) 963, 1126, 1693, 1738, 2560
VpaK11BI GGWCC 2 cut(s) 1605, 2220
XagI CCTNNNNNAGG 1 cut(s) 2474
XapI RAATTY 9 cut(s) 101, 874, 951, 1557, 1638, 1808, 2109, 2387, 2663
XbaI TCTAGA 3 cut(s) 1796, 1921, 2629
XceI RCATGY 4 cut(s) 88, 1971, 2159, 2280
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 732
XmiI GTMKAC 1 cut(s) 2370
XmnI GAANNNNTTC 1 cut(s) 2657
XspI CTAG 9 cut(s) 527, 578, 1532, 1653, 1797, 1922, 2289, 2630, 2681
ZrmI AGTACT 2 cut(s) 2063, 2365
Zsp2I ATGCAT 2 cut(s) 1335, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.