Prupe.6G055100_v2.0.a1

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001222
Physical Location & Seq
Reverse (-)
3898624 .. 3905531
6908 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G055100.2

Sequence Viewer

Length: 3819 bp
ATGGCGGGCAAGGACCTTGAAGAACTGGGTTCCCTTAGGAAGAGAAAGCGCAGGACTCAGAATAAAAAAAAGCTTCTTGTTGGTGACAAAGTTGAGGTGAGGAGTCTAGAAGATGGGTTTCAGGGGTCGTGGCATCCTGGGATTGTTACTGCATGCAAGAAGCAAGGTTGTCGTCAAGTTCAATACGACCATATTCTATATGATGACGGGTCTGGTAATCTGGTTGATGTTGTGTCTGTTTCACCCATTCTGGATGGTATTGGCTCATTCACTCGGGACTGGAGTAACTATCGTGGGTGTATAAGGCCAACACCCCCTCGCATCCAGCCAGGGGAATGGGACCTTCCCTATGGACTCTGTGTGGATGTGTATCATCTAGAGGCTTGGTGGGAAGGTGTTATCTTTGATCATGAAGATGGTTCAGAGGAGAGGAGGATCTTCTTCCCGGATTTGGGTGATGAACTAAAGGCTCGGATTGACACTTTGCGAATTACTCATGAGTGGGATGAGGTTACAGAAAATTGGAAGCGGCGTGGAACTTGGATATTTCTTGAGTTGCTTGAGCAATATAATCAGGAGAGGTATATTGCCGTTTCCGTGAAGCAAATTTGGTATGACGTGCGAGAAAAAGAACCCTTCGAGTGGACTTCTTTAATGAGACATGTGTGGGAAGAGCTGGTTTTGGAGGTCATTAATGATAACATAAGCATCACAGTTGATGAACTATTTGGGGCATTAGACAAGTCAGGATTCTTTTCACAAGAAACCCAGGTAGAATTGGAGTCAGCTGTTTTTGTTTCTGATGCTAATATGAATCCTAAAGAAAATATGGCAGACTCTTTGGCTATTGTACCTGTTGATGAGTTGCTGAATAGTGATTTGGTAGTTGATCGTGAAGATGCTGTGAATAAGGCATTGAACTGCAGTGCTGAGATTATTGATGAACGGCATCTAAATGGAGGTTTGTCTATCAATCCAGACTCTGCCTGCACGGAGCAGGTTCAAGAGAAATCTTATAGGGATCAATTGATTTCTGTGGTAGGAGATGAAGGACCAAATATGAATAGTTTGGAGTACTCCGATATATCCTTCCAAACTAAAGGGGTGTGTGTTCTACCTCAGGTTTTGTTGGTGTTTCCTCCTAATCTTGATGGAAATTCTTGCACCAATTCTGTTATCAGCAATGATGGAATTTGCAGTACTAATTACAGAGGCAGAAGTACCCTAGATTGGCGACCTTTGGACACTCCAGCTGAGTTTTGCCCTGATGCTGTTGATGAATATGCAGATTTTGTTAAGGGAATGTCTTCGAAATTCTCAACAACAGTTGTTAAGCAGCATATTTCATACTTGGGCTGGAAAATACATAGTGCAATGGATAAGGGCAGGCCCAGACTACGATACTTGTCTCCTGCTGGAGAATACGAGTACTCTCTTCGTCAAGTGTGTAAGACGTTGAAGAAACGCAAGAAAGATACACTTTTTTCAATCTCTCAAGATGCACATCAGGATTTGCATGGTTCAGCAGAAGAGTCTCTTCTCATTGAACAGCCACAAGAGATTCAACATCCCAATTATTATCCTCAGAAAGTAGAGTCTCCTTGCTCCACGGTATTTATTTTCAAACCTGAATATTGCCCTGAAGCTGTTGTGGAATATTATATGCATGCAGCAGGCAAGACAATCAAGAAGGAACTACGAAAAATGATTTCAAAAGCAAAGAAGCACCTCTCAGCTGTGGGGTGGGTGTTCGTGTACTTGAATGCAAAGTCAAGGAACTTTCACTACAGGTCACCCAGCGGAATACTGTATCGTACACTTCGAAGTGCATGCAAAAGTTGTATGGATGAAGGAGTTTCTGAAAAGAGACCTGCTGAATGCATGTATGTTGTTGAGGAAGATGAGGGCCAGTTGACAAGAAATAAATTATGTTCTGCAGCAAGTAATTTGGATTTTCAGGAAGGCTTGGTACCGTTAAAAAGTCTGTCCAAAAAGTGGTCTAGAGATTCTGGTAATGTGAAAGTTCAAGGTCGTAGAAAACGCCAAAGGAAGAGAAATAATATTTTATCAGATCTTGCACCTGACTTACTTCAAAGACAGCCTTACTTACATGGTAGAACAGATGGATCAACAAAGGATCAATGTACCAGCCCTCCAAAGTTGAAAAGGAGAAAGGTATCTGGAGCTTTGAATCGGTTAAAAAATGGTCTGGATGGTAGTCCCCCAACTCGTGTGCTTAGGTCAAGCAAATGGGTGCAGGAAGCGGTTACATCAACTTCCTCGCACAACAATCCTCGTACTGTCTTATCTTGGTTGATAGACAATAATGTGGTTTTACCAAGGGCAAAAGTACATCATCGTAGCACAAAGGATAGTCATCCAATGAAAGAAGGGAGGATAACTCGTGAGGGGATCAGGTGCAGCTGTTGCCAAGAGGTGTATACTCTTAGTTGCTTTGGGAATCATGCTGGCAGCAGTTACTGCAGTCCTTCTGCAAATATTTTTCTGGAAGATGGTAGGTCTCTCTTAGATTGCCAGGTGCAAATTATGCATGATAGAAGGAAAAGGAGCTTGCGAAAAGAACCACGTGATAGGATAAAGGGCAATTGGCACCGAGGCGAAAATGATTACATTTGTACTGTGTGTCACTATGGTGGTGACCTAATTTTATGTGATCAATGCCCATCATCATTTCACAAAAGTTGCCTTGGCTTGAAGTATGTTCCAGATGGTGACTGGTTCTGTGCATCTTGCTGTTGTGGAATTTGTGGCCAAACCAACTTCAAAGAAGATAAAGAGCCTATTATGGATGATAGTAGTGTTCTCACATGTGGTCAATGCGAGCATAAATATCACAAAGGGTGCCTGAGGAAAAGGGGTGCAGATAAGTTGGAAAGTGATCCAAAAGGAAATTGGTTTTGCACCAGAAATTGTAAAAAGATATTTTTAGGCCTCCACGAGCTTCTAGGGAAACAGATTCCAGTGGGAGATAACAATCTATCTTGGTCACTAGTCAAGTCCATTAAGTCTGATATTCATGATACTGATGAACCTCATATTGATGCCATTGAGAGTTACAGCCGACTCAATGTTGCTCTTGATGTGATGCACGAGTGTTTTGTGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGTAGAAGATATCATCTTCACTAGAGGGTCAGATCTTAACCGTCTGAACTTCCGAGGGTTCTATACTGTGCTTTTGGAGAGAAATGACGAACTAATCACTGTGGCTACTGTAAGGATCTTTGGAGGCAAGGTGGCTGAAGTACCTCTTGTTGCTACCAGATTTCAATATCGTCGACTTGGAATGTGTCGTATACTAATGGATGAACTTGAAAAGATGCTTATGCAGTTAGGCGTGGAGAGATTGGTTTTGCCAGCTGTCCCCAGTGTACTAAACACATGGACAACTTCATTTGGGTTCTCTACGATGACAGCTTCTGAGAGGCTACAGTTTCTGGATTATACTTTCTTGGACTTTCAGGGTACCATTATGTGTCAGAAACAGTTAATGAAAAACCCTCCAACTGAGGCAGTCCCTTTAGAAGGAACCCAGCTTGGTCTTTGTTTAGATACCTATGAAAGTCCTGATAATATCGATGTTGATAGGTCCAGTGCTGTCTCTGAAGTGTATCAAGCAGAACAAATTGAGGATAGCGGAATTGTGCACCAAGGAGTGGACAGAAGCAGTCCAGAGTTTTCCTCCATGGAAAATGCCATTAAAAAGAGGGCACGGGATGGGGACTACAAGCAGTACAAGCGAAGGAGGATATCCGCCAATGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

1273

Amino Acids

144.76

Weight (kDa)

6.57

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 988, 1879
Acc65I GGTACC 2 cut(s) 1969, 3515
AccB1I GGYRCC 4 cut(s) 1969, 2610, 2862, 3515
AccB7I CCANNNNNTGG 2 cut(s) 1995, 3706
AccI GTMKAC 3 cut(s) 2441, 3328, 3346
AciI CCGC 6 cut(s) 5, 529, 1800, 2264, 3687, 3804
AclWI GGATC 7 cut(s) 443, 1029, 2134, 2145, 2420, 2894, 3278
AcoI YGGCCR 1 cut(s) 2770
AcsI RAATTY 5 cut(s) 606, 1156, 1191, 1313, 2763
AcuI CTGAAG 3 cut(s) 1662, 3312, 3675
AcvI CACGTG 1 cut(s) 2588
AfiI CCNNNNNNNGG 8 cut(s) 331, 451, 452, 642, 1230, 1995, 3575, 3706
AflIII ACRYGT 2 cut(s) 661, 2828
AhlI ACTAGT 1 cut(s) 3010
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 5 cut(s) 136, 328, 768, 2535, 3143
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AleI CACNNNNGTG 1 cut(s) 2652
Alw21I GWGCWC 1 cut(s) 3699
Alw26I GTCTC 7 cut(s) 652, 1413, 1539, 1602, 1861, 2526, 3655
Alw44I GTGCAC 1 cut(s) 3695
AlwI GGATC 7 cut(s) 443, 1029, 2134, 2145, 2420, 2894, 3278
AlwNI CAGNNNCTG 4 cut(s) 983, 2481, 3470, 3487
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 5 cut(s) 305, 1388, 1906, 2770, 2950
ApaLI GTGCAC 1 cut(s) 3695
ApeKI GCWGC 5 cut(s) 1336, 1670, 1937, 2421, 2472
ApoI RAATTY 5 cut(s) 606, 1156, 1191, 1313, 2763
AseI ATTAAT 1 cut(s) 693
Asp700I GAANNNNTTC 1 cut(s) 1306
Asp718I GGTACC 2 cut(s) 1969, 3515
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 6 cut(s) 13, 340, 1052, 1389, 1906, 3639
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 7 cut(s) 95, 109, 234, 467, 1785, 2669, 2744
AsuII TTCGAA 2 cut(s) 1310, 1822
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 4 cut(s) 13, 340, 1052, 3639
AxyI CCTNAGG 3 cut(s) 35, 1119, 2867
BaeGI GKGCMC 2 cut(s) 3699, 3763
BalI TGGCCA 1 cut(s) 2772
BanI GGYRCC 4 cut(s) 1969, 2610, 2862, 3515
BarI GAAGNNNNNNTAC 2 cut(s) 1953, 1985
BauI CACGAG 4 cut(s) 2229, 2403, 2957, 3110
BbrPI CACGTG 1 cut(s) 2588
BbsI GAAGAC 1 cut(s) 1299
Bbv12I GWGCWC 1 cut(s) 3699
BbvI GCAGC 5 cut(s) 1348, 1682, 1949, 2433, 2484
BceAI ACGGC 2 cut(s) 575, 962
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 1812, 1846
BciT130I CCWGG 5 cut(s) 138, 330, 770, 2537, 3145
BclI TGATCA 2 cut(s) 406, 2674
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 7 cut(s) 652, 1413, 1539, 1602, 1861, 2526, 3655
BcuI ACTAGT 1 cut(s) 3010
BfaI CTAG 7 cut(s) 107, 377, 1226, 2001, 2966, 3011, 3177
BfmI CTRYAG 5 cut(s) 922, 1786, 1935, 2482, 3479
BfuAI ACCTGC 2 cut(s) 988, 1879
BglII AGATCT 3 cut(s) 2071, 3151, 3187
BisI GCNGC 6 cut(s) 530, 1337, 1671, 1938, 2422, 2473
BlsI GCNGC 6 cut(s) 531, 1338, 1672, 1939, 2423, 2474
BmcAI AGTACT 3 cut(s) 1076, 1201, 1430
Bme1390I CCNGG 6 cut(s) 138, 330, 446, 770, 2537, 3145
Bme18I GGWCC 4 cut(s) 13, 340, 1052, 3639
BmeT110I CYCGRG 1 cut(s) 273
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 6 cut(s) 13, 340, 1052, 1389, 1906, 3639
BmiI GGNNCC 8 cut(s) 31, 341, 1971, 2612, 2864, 3135, 3517, 3580
BmrFI CCNGG 6 cut(s) 138, 330, 446, 770, 2537, 3145
BmrI ACTGGG 2 cut(s) 35, 3411
BmuI ACTGGG 2 cut(s) 35, 3411
BpiI GAAGAC 1 cut(s) 1299
BplI GAGNNNNNCTC 4 cut(s) 2386, 2418, 3716, 3748
BpmI CTGGAG 4 cut(s) 301, 1233, 1437, 2202
Bpu10I CCTNAGC 1 cut(s) 2237
Bpu14I TTCGAA 2 cut(s) 1310, 1822
BpuEI CTTGAG 3 cut(s) 572, 581, 1479
BpuMI CCSGG 1 cut(s) 446
Bsa29I ATCGAT 1 cut(s) 3627
BsaAI YACGTR 1 cut(s) 2588
BsaBI GATNNNNATC 4 cut(s) 369, 1503, 2376, 2994
BsaI GGTCTC 2 cut(s) 1861, 2526
BsaXI ACNNNNNCTCC 4 cut(s) 2137, 2167, 3270, 3300
Bsc4I CCNNNNNNNGG 8 cut(s) 331, 451, 452, 642, 1230, 1995, 3575, 3706
Bse1I ACTGG 7 cut(s) 30, 284, 1909, 2741, 2981, 3417, 3642
Bse21I CCTNAGG 3 cut(s) 35, 1119, 2867
Bse3DI GCAATG 2 cut(s) 1189, 1380
Bse8I GATNNNNATC 4 cut(s) 369, 1503, 2376, 2994
BseBI CCWGG 5 cut(s) 138, 330, 770, 2537, 3145
BseCI ATCGAT 1 cut(s) 3627
BseJI GATNNNNATC 4 cut(s) 369, 1503, 2376, 2994
BseLI CCNNNNNNNGG 8 cut(s) 331, 451, 452, 642, 1230, 1995, 3575, 3706
BseMI GCAATG 2 cut(s) 1189, 1380
BseMII CTCAG 9 cut(s) 71, 921, 1133, 1245, 1598, 1746, 2858, 3462, 3549
BseNI ACTGG 7 cut(s) 30, 284, 1909, 2741, 2981, 3417, 3642
BseRI GAGGAG 3 cut(s) 115, 440, 445
BseSI GKGCMC 2 cut(s) 3699, 3763
BseXI GCAGC 5 cut(s) 1348, 1682, 1949, 2433, 2484
BseYI CCCAGC 2 cut(s) 1796, 3582
BsgI GTGCAG 4 cut(s) 973, 2276, 2440, 2901
BshFI GGCC 5 cut(s) 307, 1390, 1908, 2772, 2952
BshNI GGYRCC 4 cut(s) 1969, 2610, 2862, 3515
BshVI ATCGAT 1 cut(s) 3627
BsiHKAI GWGCWC 1 cut(s) 3699
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 446
BslFI GGGAC 6 cut(s) 290, 353, 2205, 3398, 3551, 3785
BslI CCNNNNNNNGG 8 cut(s) 331, 451, 452, 642, 1230, 1995, 3575, 3706
BsmAI GTCTC 7 cut(s) 652, 1413, 1539, 1602, 1861, 2526, 3655
BsmFI GGGAC 6 cut(s) 290, 353, 2205, 3398, 3551, 3785
BsmI GAATGC 2 cut(s) 1768, 1883
BsnI GGCC 5 cut(s) 307, 1390, 1908, 2772, 2952
Bso31I GGTCTC 2 cut(s) 1861, 2526
BsoBI CYCGRG 1 cut(s) 273
Bsp119I TTCGAA 2 cut(s) 1310, 1822
Bsp1286I GDGCHC 2 cut(s) 3699, 3763
Bsp19I CCATGG 1 cut(s) 3735
BspACI CCGC 6 cut(s) 5, 529, 1800, 2264, 3687, 3804
BspANI GGCC 5 cut(s) 307, 1390, 1908, 2772, 2952
BspCNI CTCAG 9 cut(s) 70, 922, 1132, 1246, 1597, 1745, 2859, 3463, 3550
BspDI ATCGAT 1 cut(s) 3627
BspHI TCATGA 3 cut(s) 409, 496, 3037
BspLI GGNNCC 8 cut(s) 31, 341, 1971, 2612, 2864, 3135, 3517, 3580
BspMAI CTGCAG 3 cut(s) 926, 1939, 2486
BspMI ACCTGC 2 cut(s) 988, 1879
BspPI GGATC 7 cut(s) 443, 1029, 2134, 2145, 2420, 2894, 3278
BspQI GCTCTTC 1 cut(s) 666
BspT104I TTCGAA 2 cut(s) 1310, 1822
BspT107I GGYRCC 4 cut(s) 1969, 2610, 2862, 3515
BspTNI GGTCTC 2 cut(s) 1861, 2526
BsrDI GCAATG 2 cut(s) 1189, 1380
BsrI ACTGG 7 cut(s) 30, 284, 1909, 2741, 2981, 3417, 3642
BssNAI GTATAC 2 cut(s) 2442, 3347
BssSI CACGAG 4 cut(s) 2229, 2403, 2957, 3110
BssT1I CCWWGG 4 cut(s) 2339, 2707, 3700, 3735
Bst1107I GTATAC 2 cut(s) 2442, 3347
Bst2BI CACGAG 4 cut(s) 2229, 2403, 2957, 3110
Bst2UI CCWGG 5 cut(s) 138, 330, 770, 2537, 3145
Bst6I CTCTTC 6 cut(s) 35, 666, 1440, 1524, 1542, 2045
BstAPI GCANNNNNTGC 3 cut(s) 2427, 2481, 2548
BstBAI YACGTR 1 cut(s) 2588
BstBI TTCGAA 2 cut(s) 1310, 1822
BstDSI CCRYGG 2 cut(s) 1608, 3735
BstEII GGTNACC 2 cut(s) 1791, 2657
BstENI CCTNNNNNAGG 1 cut(s) 3573
BstHHI GCGC 1 cut(s) 51
BstMAI GTCTC 7 cut(s) 652, 1413, 1539, 1602, 1861, 2526, 3655
BstMWI GCNNNNNNNGC 4 cut(s) 2427, 2481, 2548, 3787
BstNI CCWGG 5 cut(s) 138, 330, 770, 2537, 3145
BstNSI RCATGY 6 cut(s) 156, 665, 1670, 1833, 1885, 2832
BstPI GGTNACC 2 cut(s) 1791, 2657
BstSCI CCNGG 6 cut(s) 136, 328, 444, 768, 2535, 3143
BstSFI CTRYAG 5 cut(s) 922, 1786, 1935, 2482, 3479
BstSLI GKGCMC 2 cut(s) 3699, 3763
BstV1I GCAGC 5 cut(s) 1348, 1682, 1949, 2433, 2484
BstV2I GAAGAC 1 cut(s) 1299
BstX2I RGATCY 5 cut(s) 435, 2071, 3151, 3187, 3270
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 5 cut(s) 435, 2071, 3151, 3187, 3270
BstZ17I GTATAC 2 cut(s) 2442, 3347
Bsu15I ATCGAT 1 cut(s) 3627
Bsu36I CCTNAGG 3 cut(s) 35, 1119, 2867
BsuRI GGCC 5 cut(s) 307, 1390, 1908, 2772, 2952
BsuTUI ATCGAT 1 cut(s) 3627
BtgI CCRYGG 2 cut(s) 1608, 3735
BtrI CACGTC 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 931
BtsIMutI CAGTG 5 cut(s) 931, 2988, 3252, 3424, 3649
BveI ACCTGC 2 cut(s) 988, 1879
CaiI CAGNNNCTG 4 cut(s) 983, 2481, 3470, 3487
CciI TCATGA 3 cut(s) 409, 496, 3037
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 6 cut(s) 13, 340, 1052, 1389, 1906, 3639
ClaI ATCGAT 1 cut(s) 3627
EaeI YGGCCR 1 cut(s) 2770
Eam1104I CTCTTC 6 cut(s) 35, 666, 1440, 1524, 1542, 2045
EarI CTCTTC 6 cut(s) 35, 666, 1440, 1524, 1542, 2045
EciI GGCGGA 1 cut(s) 3793
Eco130I CCWWGG 4 cut(s) 2339, 2707, 3700, 3735
Eco147I AGGCCT 1 cut(s) 2952
Eco31I GGTCTC 2 cut(s) 1861, 2526
Eco32I GATATC 2 cut(s) 3166, 3801
Eco47I GGWCC 4 cut(s) 13, 340, 1052, 3639
Eco57I CTGAAG 3 cut(s) 1662, 3312, 3675
Eco72I CACGTG 1 cut(s) 2588
Eco81I CCTNAGG 3 cut(s) 35, 1119, 2867
Eco88I CYCGRG 1 cut(s) 273
Eco91I GGTNACC 2 cut(s) 1791, 2657
EcoNI CCTNNNNNAGG 1 cut(s) 3573
EcoO109I RGGNCCY 2 cut(s) 13, 340
EcoO65I GGTNACC 2 cut(s) 1791, 2657
EcoRII CCWGG 5 cut(s) 136, 328, 768, 2535, 3143
EcoRV GATATC 2 cut(s) 3166, 3801
EcoT14I CCWWGG 4 cut(s) 2339, 2707, 3700, 3735
EcoT22I ATGCAT 3 cut(s) 1668, 1883, 2553
ErhI CCWWGG 4 cut(s) 2339, 2707, 3700, 3735
FalI AAGNNNNNCTT 8 cut(s) 1464, 1496, 1521, 1553, 2086, 2118, 3285, 3317
FaqI GGGAC 6 cut(s) 290, 353, 2205, 3398, 3551, 3785
FbaI TGATCA 2 cut(s) 406, 2674
FblI GTMKAC 3 cut(s) 2441, 3328, 3346
Fnu4HI GCNGC 6 cut(s) 530, 1337, 1671, 1938, 2422, 2473
Fsp4HI GCNGC 6 cut(s) 530, 1337, 1671, 1938, 2422, 2473
FspBI CTAG 7 cut(s) 107, 377, 1226, 2001, 2966, 3011, 3177
GlaI GCGC 1 cut(s) 50
GluI GCNGC 6 cut(s) 530, 1337, 1671, 1938, 2422, 2473
GsaI CCCAGC 2 cut(s) 1800, 3586
GsuI CTGGAG 4 cut(s) 301, 1233, 1437, 2202
HaeIII GGCC 5 cut(s) 307, 1390, 1908, 2772, 2952
HapII CCGG 1 cut(s) 446
HhaI GCGC 1 cut(s) 51
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 2 cut(s) 1914, 3329
HindII GTYRAC 2 cut(s) 1914, 3329
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 1 cut(s) 446
HphI GGTGA 7 cut(s) 95, 109, 234, 467, 1785, 2669, 2744
Hpy99I CGWCG 1 cut(s) 3330
HpyCH4IV ACGT 3 cut(s) 618, 1454, 2587
HpyF10VI GCNNNNNNNGC 4 cut(s) 2427, 2481, 2548, 3787
HpySE526I ACGT 3 cut(s) 618, 1454, 2587
HspAI GCGC 1 cut(s) 49
KpnI GGTACC 2 cut(s) 1973, 3519
Ksp22I TGATCA 2 cut(s) 406, 2674
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 4 cut(s) 994, 1610, 2183, 2568
Lsp1109I GCAGC 5 cut(s) 1348, 1682, 1949, 2433, 2484
MaeI CTAG 7 cut(s) 107, 377, 1226, 2001, 2966, 3011, 3177
MaeII ACGT 3 cut(s) 618, 1454, 2587
MfeI CAATTG 2 cut(s) 1025, 2605
MflI RGATCY 5 cut(s) 435, 2071, 3151, 3187, 3270
MhlI GDGCHC 2 cut(s) 3699, 3763
MlsI TGGCCA 1 cut(s) 2772
MluNI TGGCCA 1 cut(s) 2772
MlyI GAGTC 9 cut(s) 49, 112, 348, 791, 830, 974, 1541, 1604, 3078
MmeI TCCRAC 2 cut(s) 2871, 3578
Mox20I TGGCCA 1 cut(s) 2772
Mph1103I ATGCAT 3 cut(s) 1668, 1883, 2553
MroXI GAANNNNTTC 1 cut(s) 1306
MscI TGGCCA 1 cut(s) 2772
MslI CAYNNNNRTG 4 cut(s) 414, 954, 2652, 3060
Msp20I TGGCCA 1 cut(s) 2772
MspA1I CMGCKG 6 cut(s) 788, 1253, 1736, 1800, 2424, 3410
MspI CCGG 1 cut(s) 446
MspR9I CCNGG 6 cut(s) 138, 330, 446, 770, 2537, 3145
MunI CAATTG 2 cut(s) 1025, 2605
Mva1269I GAATGC 2 cut(s) 1768, 1883
MvaI CCWGG 5 cut(s) 138, 330, 770, 2537, 3145
MwoI GCNNNNNNNGC 4 cut(s) 2427, 2481, 2548, 3787
NciI CCSGG 1 cut(s) 446
NcoI CCATGG 1 cut(s) 3735
NlaIV GGNNCC 8 cut(s) 31, 341, 1971, 2612, 2864, 3135, 3517, 3580
NmuCI GTSAC 6 cut(s) 83, 1791, 2645, 2657, 2732, 3006
NsiI ATGCAT 3 cut(s) 1668, 1883, 2553
NspI RCATGY 6 cut(s) 156, 665, 1670, 1833, 1885, 2832
NspV TTCGAA 2 cut(s) 1310, 1822
OliI CACNNNNGTG 1 cut(s) 2652
PaeI GCATGC 3 cut(s) 156, 1670, 1833
PagI TCATGA 3 cut(s) 409, 496, 3037
PceI AGGCCT 1 cut(s) 2952
PciI ACATGT 2 cut(s) 661, 2828
PciSI GCTCTTC 1 cut(s) 666
PcsI WCGNNNNNNNCGW 1 cut(s) 1819
PctI GAATGC 2 cut(s) 1768, 1883
PdmI GAANNNNTTC 1 cut(s) 1306
PfeI GAWTC 7 cut(s) 750, 814, 1561, 2006, 2191, 2461, 2977
PflMI CCANNNNNTGG 2 cut(s) 1995, 3706
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 6 cut(s) 531, 1338, 1672, 1939, 2423, 2474
PleI GAGTC 9 cut(s) 49, 111, 348, 790, 830, 974, 1540, 1603, 3078
PmaCI CACGTG 1 cut(s) 2588
PmlI CACGTG 1 cut(s) 2588
PpsI GAGTC 9 cut(s) 49, 111, 348, 790, 830, 974, 1540, 1603, 3078
Ppu21I YACGTR 1 cut(s) 2588
PpuMI RGGWCCY 2 cut(s) 13, 340
PscI ACATGT 2 cut(s) 661, 2828
PshBI ATTAAT 1 cut(s) 693
Psp5II RGGWCCY 2 cut(s) 13, 340
Psp6I CCWGG 5 cut(s) 136, 328, 768, 2535, 3143
PspCI CACGTG 1 cut(s) 2588
PspEI GGTNACC 2 cut(s) 1791, 2657
PspFI CCCAGC 2 cut(s) 1796, 3582
PspGI CCWGG 5 cut(s) 136, 328, 768, 2535, 3143
PspN4I GGNNCC 8 cut(s) 31, 341, 1971, 2612, 2864, 3135, 3517, 3580
PspPI GGNCC 6 cut(s) 13, 340, 1052, 1389, 1906, 3639
PspPPI RGGWCCY 2 cut(s) 13, 340
PsrI GAACNNNNNNTAC 2 cut(s) 1769, 1801
PstI CTGCAG 3 cut(s) 926, 1939, 2486
PstNI CAGNNNCTG 4 cut(s) 983, 2481, 3470, 3487
PsuI RGATCY 5 cut(s) 435, 2071, 3151, 3187, 3270
PvuII CAGCTG 5 cut(s) 788, 1253, 1736, 2424, 3410
RseI CAYNNNNRTG 4 cut(s) 414, 954, 2652, 3060
SalI GTCGAC 1 cut(s) 3327
SapI GCTCTTC 1 cut(s) 666
SatI GCNGC 6 cut(s) 530, 1337, 1671, 1938, 2422, 2473
Sau96I GGNCC 6 cut(s) 13, 340, 1052, 1389, 1906, 3639
ScaI AGTACT 3 cut(s) 1076, 1201, 1430
SchI GAGTC 9 cut(s) 49, 112, 348, 791, 830, 974, 1541, 1604, 3078
ScrFI CCNGG 6 cut(s) 138, 330, 446, 770, 2537, 3145
SduI GDGCHC 2 cut(s) 3699, 3763
SfcI CTRYAG 5 cut(s) 922, 1786, 1935, 2482, 3479
SfuI TTCGAA 2 cut(s) 1310, 1822
SinI GGWCC 4 cut(s) 13, 340, 1052, 3639
SmiMI CAYNNNNRTG 4 cut(s) 414, 954, 2652, 3060
SmlI CTYRAG 3 cut(s) 551, 560, 1494
SmoI CTYRAG 3 cut(s) 551, 560, 1494
SpeI ACTAGT 1 cut(s) 3010
SphI GCATGC 3 cut(s) 156, 1670, 1833
SseBI AGGCCT 1 cut(s) 2952
SsiI CCGC 6 cut(s) 5, 529, 1800, 2264, 3687, 3804
SspI AATATT 4 cut(s) 1634, 1658, 2062, 2500
SspMI CTAG 7 cut(s) 107, 377, 1226, 2001, 2966, 3011, 3177
StuI AGGCCT 1 cut(s) 2952
StyD4I CCNGG 6 cut(s) 136, 328, 444, 768, 2535, 3143
StyI CCWWGG 4 cut(s) 2339, 2707, 3700, 3735
TaiI ACGT 3 cut(s) 621, 1457, 2590
TaqI TCGA 5 cut(s) 639, 1310, 1822, 3328, 3627
TatI WGTACW 8 cut(s) 1074, 1199, 1428, 1755, 2350, 2636, 3421, 3783
TauI GCSGC 1 cut(s) 532
TfiI GAWTC 7 cut(s) 750, 814, 1561, 2006, 2191, 2461, 2977
TscAI CASTG 5 cut(s) 931, 2988, 3259, 3424, 3649
TseFI GTSAC 6 cut(s) 83, 1791, 2645, 2657, 2732, 3006
TseI GCWGC 5 cut(s) 1336, 1670, 1937, 2421, 2472
Tsp45I GTSAC 6 cut(s) 83, 1791, 2645, 2657, 2732, 3006
TspGWI ACGGA 2 cut(s) 586, 1007
TspRI CASTG 5 cut(s) 931, 2988, 3259, 3424, 3649
Van91I CCANNNNNTGG 2 cut(s) 1995, 3706
VneI GTGCAC 1 cut(s) 3695
VpaK11BI GGWCC 4 cut(s) 13, 340, 1052, 3639
VspI ATTAAT 1 cut(s) 693
XagI CCTNNNNNAGG 1 cut(s) 3573
XapI RAATTY 5 cut(s) 606, 1156, 1191, 1313, 2763
XbaI TCTAGA 3 cut(s) 106, 376, 2000
XceI RCATGY 6 cut(s) 156, 665, 1670, 1833, 1885, 2832
XcmI CCANNNNNNNNNTGG 2 cut(s) 2733, 2910
XmiI GTMKAC 3 cut(s) 2441, 3328, 3346
XmnI GAANNNNTTC 1 cut(s) 1306
XspI CTAG 7 cut(s) 107, 377, 1226, 2001, 2966, 3011, 3177
ZrmI AGTACT 3 cut(s) 1076, 1201, 1430
Zsp2I ATGCAT 3 cut(s) 1668, 1883, 2553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.