ubiquitinyl hydrolase activity
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TDBD | PF16135 | 126 - 183 | 3.9e-18 | Tify domain binding domain |
| PHD | PF00628 | 214 - 253 | 1.4e-07 | PHD-finger |
| IDM1_C | PF23209 | 370 - 508 | 3.9e-50 | Increased DNA methylation 1, C-terminal domain |
| MATH_2 | PF22486 | 546 - 669 | 8.2e-30 | MATH domain |
| MATH | PF00917 | 550 - 671 | 4.6e-11 | MATH domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 502 |
| Acc65I | GGTACC | 2 cut(s) | 1298, 2300 |
| AccB1I | GGYRCC | 3 cut(s) | 858, 1298, 2300 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1295 |
| AccI | GTMKAC | 2 cut(s) | 1755, 1885 |
| AciI | CCGC | 4 cut(s) | 980, 1720, 2387, 2750 |
| AclWI | GGATC | 5 cut(s) | 24, 890, 1185, 1268, 1528 |
| AcoI | YGGCCR | 1 cut(s) | 775 |
| AcsI | RAATTY | 5 cut(s) | 1304, 1369, 2025, 2330, 2577 |
| AcuI | CTGAAG | 5 cut(s) | 1838, 2070, 2196, 2359, 2615 |
| AdeI | CACNNNGTG | 1 cut(s) | 858 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1133, 1295, 1562, 1964, 2326, 2419 |
| AflII | CTTAAG | 1 cut(s) | 553 |
| AflIII | ACRYGT | 3 cut(s) | 1888, 1985, 2152 |
| AhdI | GACNNNNNGTC | 3 cut(s) | 384, 1423, 1762 |
| AjnI | CCWGG | 4 cut(s) | 619, 998, 2055, 2256 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1788, 1820, 2068, 2100 |
| AleI | CACNNNNGTG | 1 cut(s) | 1807 |
| Alw21I | GWGCWC | 2 cut(s) | 1273, 2647 |
| Alw26I | GTCTC | 3 cut(s) | 1055, 1378, 1636 |
| AlwI | GGATC | 5 cut(s) | 24, 890, 1185, 1268, 1528 |
| Ama87I | CYCGRG | 1 cut(s) | 2417 |
| AoxI | GGCC | 5 cut(s) | 775, 946, 2053, 2598, 2739 |
| ApeKI | GCWGC | 8 cut(s) | 456, 477, 497, 576, 1860, 2090, 2693, 2753 |
| ApoI | RAATTY | 5 cut(s) | 1304, 1369, 2025, 2330, 2577 |
| AseI | ATTAAT | 1 cut(s) | 1340 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2600 |
| Asp718I | GGTACC | 2 cut(s) | 1298, 2300 |
| AspLEI | GCGC | 1 cut(s) | 1860 |
| AspS9I | GGNCC | 1 cut(s) | 51 |
| AsuHPI | GGTGA | 2 cut(s) | 749, 1634 |
| AsuII | TTCGAA | 1 cut(s) | 2790 |
| AvaI | CYCGRG | 1 cut(s) | 2417 |
| AvaII | GGWCC | 1 cut(s) | 51 |
| AxyI | CCTNAGG | 1 cut(s) | 863 |
| BalI | TGGCCA | 1 cut(s) | 777 |
| BanI | GGYRCC | 3 cut(s) | 858, 1298, 2300 |
| BanII | GRGCYC | 1 cut(s) | 2647 |
| BauI | CACGAG | 3 cut(s) | 213, 953, 1100 |
| BbsI | GAAGAC | 1 cut(s) | 162 |
| Bbv12I | GWGCWC | 2 cut(s) | 1273, 2647 |
| BbvCI | CCTCAGC | 2 cut(s) | 221, 691 |
| BbvI | GCAGC | 8 cut(s) | 443, 464, 509, 563, 1872, 2102, 2705, 2765 |
| BccI | CCATC | 7 cut(s) | 512, 650, 728, 1061, 1564, 1710, 2000 |
| BceAI | ACGGC | 2 cut(s) | 1070, 2111 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 110, 144 |
| BciT130I | CCWGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| BciVI | GTATCC | 3 cut(s) | 173, 1495, 1743 |
| BcoDI | GTCTC | 3 cut(s) | 1055, 1378, 1636 |
| BfaI | CTAG | 8 cut(s) | 1082, 1415, 1475, 1541, 1815, 1959, 2022, 2232 |
| BfmI | CTRYAG | 6 cut(s) | 457, 478, 495, 577, 1466, 2274 |
| BfrI | CTTAAG | 1 cut(s) | 553 |
| BfuAI | ACCTGC | 1 cut(s) | 502 |
| BfuI | GTATCC | 3 cut(s) | 173, 1495, 1743 |
| BglII | AGATCT | 2 cut(s) | 1141, 2203 |
| BisI | GCNGC | 9 cut(s) | 457, 478, 498, 577, 1861, 2091, 2694, 2751, 2754 |
| BlsI | GCNGC | 9 cut(s) | 458, 479, 499, 578, 1862, 2092, 2695, 2752, 2755 |
| BmcAI | AGTACT | 2 cut(s) | 1685, 2308 |
| Bme1390I | CCNGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| Bme18I | GGWCC | 1 cut(s) | 51 |
| BmeRI | GACNNNNNGTC | 3 cut(s) | 384, 1423, 1762 |
| BmeT110I | CYCGRG | 1 cut(s) | 2417 |
| BmgT120I | GGNCC | 1 cut(s) | 51 |
| BmiI | GGNNCC | 3 cut(s) | 860, 1300, 2302 |
| BmrFI | CCNGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| BmrI | ACTGGG | 2 cut(s) | 864, 2470 |
| BmuI | ACTGGG | 2 cut(s) | 864, 2470 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2126 |
| BpiI | GAAGAC | 1 cut(s) | 162 |
| BpmI | CTGGAG | 2 cut(s) | 603, 2078 |
| Bpu10I | CCTNAGC | 2 cut(s) | 221, 691 |
| Bpu14I | TTCGAA | 1 cut(s) | 2790 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1182 |
| BsaJI | CCNNGG | 3 cut(s) | 1839, 2256, 2502 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1260, 1290, 1900, 1930 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1133, 1295, 1562, 1964, 2326, 2419 |
| Bse21I | CCTNAGG | 1 cut(s) | 863 |
| Bse3DI | GCAATG | 1 cut(s) | 91 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1182 |
| BseBI | CCWGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| BseDI | CCNNGG | 3 cut(s) | 1839, 2256, 2502 |
| BseGI | GGATG | 5 cut(s) | 361, 730, 1925, 2074, 2437 |
| BseJI | GATNNNNATC | 1 cut(s) | 1182 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1133, 1295, 1562, 1964, 2326, 2419 |
| BseMI | GCAATG | 1 cut(s) | 91 |
| BseMII | CTCAG | 7 cut(s) | 212, 705, 854, 1472, 1806, 1843, 2175 |
| BseRI | GAGGAG | 1 cut(s) | 586 |
| BseXI | GCAGC | 8 cut(s) | 443, 464, 509, 563, 1872, 2102, 2705, 2765 |
| BsgI | GTGCAG | 2 cut(s) | 260, 897 |
| BshFI | GGCC | 5 cut(s) | 777, 948, 2055, 2600, 2741 |
| BshNI | GGYRCC | 3 cut(s) | 858, 1298, 2300 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1273, 2647 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2417 |
| BslFI | GGGAC | 4 cut(s) | 391, 672, 1388, 2457 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1133, 1295, 1562, 1964, 2326, 2419 |
| BsmAI | GTCTC | 3 cut(s) | 1055, 1378, 1636 |
| BsmFI | GGGAC | 4 cut(s) | 391, 672, 1388, 2457 |
| BsmI | GAATGC | 2 cut(s) | 695, 2459 |
| BsnI | GGCC | 5 cut(s) | 777, 948, 2055, 2600, 2741 |
| BsoBI | CYCGRG | 1 cut(s) | 2417 |
| Bsp119I | TTCGAA | 1 cut(s) | 2790 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1273, 2647 |
| Bsp1407I | TGTACA | 1 cut(s) | 281 |
| Bsp19I | CCATGG | 1 cut(s) | 1839 |
| BspACI | CCGC | 4 cut(s) | 980, 1720, 2387, 2750 |
| BspANI | GGCC | 5 cut(s) | 777, 948, 2055, 2600, 2741 |
| BspCNI | CTCAG | 7 cut(s) | 213, 704, 855, 1471, 1805, 1842, 2176 |
| BspLI | GGNNCC | 3 cut(s) | 860, 1300, 2302 |
| BspMAI | CTGCAG | 5 cut(s) | 461, 482, 499, 581, 2278 |
| BspMI | ACCTGC | 1 cut(s) | 502 |
| BspPI | GGATC | 5 cut(s) | 24, 890, 1185, 1268, 1528 |
| BspT104I | TTCGAA | 1 cut(s) | 2790 |
| BspT107I | GGYRCC | 3 cut(s) | 858, 1298, 2300 |
| BspTI | CTTAAG | 1 cut(s) | 553 |
| BsrDI | GCAATG | 1 cut(s) | 91 |
| BsrGI | TGTACA | 1 cut(s) | 281 |
| BssECI | CCNNGG | 3 cut(s) | 1839, 2256, 2502 |
| BssSI | CACGAG | 3 cut(s) | 213, 953, 1100 |
| BssT1I | CCWWGG | 2 cut(s) | 1839, 2502 |
| Bst2BI | CACGAG | 3 cut(s) | 213, 953, 1100 |
| Bst2UI | CCWGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| Bst4CI | ACNGT | 9 cut(s) | 410, 646, 992, 1022, 1196, 1255, 1402, 1916, 2011 |
| Bst6I | CTCTTC | 3 cut(s) | 563, 2459, 2787 |
| BstAFI | CTTAAG | 1 cut(s) | 553 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 411, 2273, 2498 |
| BstAUI | TGTACA | 1 cut(s) | 281 |
| BstBI | TTCGAA | 1 cut(s) | 2790 |
| BstC8I | GCNNGC | 6 cut(s) | 454, 757, 839, 1073, 2095, 2748 |
| BstDEI | CTNAG | 9 cut(s) | 221, 532, 691, 863, 943, 1458, 1792, 1829, 2184 |
| BstDSI | CCRYGG | 1 cut(s) | 1839 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 1962, 2324, 2417 |
| BstF5I | GGATG | 5 cut(s) | 361, 730, 1925, 2074, 2437 |
| BstHHI | GCGC | 1 cut(s) | 1860 |
| BstMAI | GTCTC | 3 cut(s) | 1055, 1378, 1636 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 151, 411, 924, 1277, 2099, 2273, 2498, 2747 |
| BstNI | CCWGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| BstNSI | RCATGY | 3 cut(s) | 1892, 1989, 2042 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2126 |
| BstSCI | CCNGG | 4 cut(s) | 619, 998, 2055, 2256 |
| BstSFI | CTRYAG | 6 cut(s) | 457, 478, 495, 577, 1466, 2274 |
| BstV1I | GCAGC | 8 cut(s) | 443, 464, 509, 563, 1872, 2102, 2705, 2765 |
| BstV2I | GAAGAC | 1 cut(s) | 162 |
| BstX2I | RGATCY | 4 cut(s) | 1141, 1260, 1533, 2203 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1809, 2051 |
| BstYI | RGATCY | 4 cut(s) | 1141, 1260, 1533, 2203 |
| Bsu36I | CCTNAGG | 1 cut(s) | 863 |
| BsuI | GTATCC | 3 cut(s) | 173, 1495, 1743 |
| BsuRI | GGCC | 5 cut(s) | 777, 948, 2055, 2600, 2741 |
| BtgI | CCRYGG | 1 cut(s) | 1839 |
| BtsCI | GGATG | 5 cut(s) | 361, 730, 1925, 2074, 2437 |
| BtsI | GCAGTG | 1 cut(s) | 466 |
| BtsIMutI | CAGTG | 5 cut(s) | 466, 852, 1398, 1701, 2481 |
| BveI | ACCTGC | 1 cut(s) | 502 |
| Cac8I | GCNNGC | 6 cut(s) | 454, 757, 839, 1073, 2095, 2748 |
| CfoI | GCGC | 1 cut(s) | 1860 |
| Cfr13I | GGNCC | 1 cut(s) | 51 |
| CseI | GACGC | 1 cut(s) | 156 |
| CsiI | ACCWGGT | 1 cut(s) | 998 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 689, 724 |
| DdeI | CTNAG | 9 cut(s) | 221, 532, 691, 863, 943, 1458, 1792, 1829, 2184 |
| DraIII | CACNNNGTG | 1 cut(s) | 858 |
| DriI | GACNNNNNGTC | 3 cut(s) | 384, 1423, 1762 |
| EaeI | YGGCCR | 1 cut(s) | 775 |
| Eam1104I | CTCTTC | 3 cut(s) | 563, 2459, 2787 |
| Eam1105I | GACNNNNNGTC | 3 cut(s) | 384, 1423, 1762 |
| EarI | CTCTTC | 3 cut(s) | 563, 2459, 2787 |
| EciI | GGCGGA | 2 cut(s) | 1735, 2402 |
| Ecl136II | GAGCTC | 1 cut(s) | 2645 |
| Eco130I | CCWWGG | 2 cut(s) | 1839, 2502 |
| Eco147I | AGGCCT | 2 cut(s) | 948, 2600 |
| Eco24I | GRGCYC | 1 cut(s) | 2647 |
| Eco32I | GATATC | 1 cut(s) | 1156 |
| Eco47I | GGWCC | 1 cut(s) | 51 |
| Eco53kI | GAGCTC | 1 cut(s) | 2645 |
| Eco57I | CTGAAG | 5 cut(s) | 1838, 2070, 2196, 2359, 2615 |
| Eco81I | CCTNAGG | 1 cut(s) | 863 |
| Eco88I | CYCGRG | 1 cut(s) | 2417 |
| EcoICRI | GAGCTC | 1 cut(s) | 2645 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 1962, 2324, 2417 |
| EcoRI | GAATTC | 1 cut(s) | 2025 |
| EcoRII | CCWGG | 4 cut(s) | 619, 998, 2055, 2256 |
| EcoRV | GATATC | 1 cut(s) | 1156 |
| EcoT14I | CCWWGG | 2 cut(s) | 1839, 2502 |
| EcoT22I | ATGCAT | 2 cut(s) | 816, 1347 |
| EcoT38I | GRGCYC | 1 cut(s) | 2647 |
| ErhI | CCWWGG | 2 cut(s) | 1839, 2502 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 926, 958, 1956, 1988 |
| FaqI | GGGAC | 4 cut(s) | 391, 672, 1388, 2457 |
| FauI | CCCGC | 1 cut(s) | 973 |
| FauNDI | CATATG | 2 cut(s) | 2244, 2557 |
| FblI | GTMKAC | 2 cut(s) | 1755, 1885 |
| Fnu4HI | GCNGC | 9 cut(s) | 457, 478, 498, 577, 1861, 2091, 2694, 2751, 2754 |
| FokI | GGATG | 5 cut(s) | 348, 737, 1912, 2081, 2424 |
| FriOI | GRGCYC | 1 cut(s) | 2647 |
| Fsp4HI | GCNGC | 9 cut(s) | 457, 478, 498, 577, 1861, 2091, 2694, 2751, 2754 |
| FspBI | CTAG | 8 cut(s) | 1082, 1415, 1475, 1541, 1815, 1959, 2022, 2232 |
| GlaI | GCGC | 1 cut(s) | 1859 |
| GluI | GCNGC | 9 cut(s) | 457, 478, 498, 577, 1861, 2091, 2694, 2751, 2754 |
| GsuI | CTGGAG | 2 cut(s) | 603, 2078 |
| HaeIII | GGCC | 5 cut(s) | 777, 948, 2055, 2600, 2741 |
| HgaI | GACGC | 1 cut(s) | 156 |
| HhaI | GCGC | 1 cut(s) | 1860 |
| Hin6I | GCGC | 1 cut(s) | 1858 |
| HinP1I | GCGC | 1 cut(s) | 1858 |
| HindIII | AAGCTT | 1 cut(s) | 2744 |
| HphI | GGTGA | 2 cut(s) | 749, 1634 |
| Hpy166II | GTNNAC | 6 cut(s) | 51, 767, 1756, 1886, 2158, 2481 |
| Hpy8I | GTNNAC | 6 cut(s) | 51, 767, 1756, 1886, 2158, 2481 |
| Hpy99I | CGWCG | 1 cut(s) | 1320 |
| HpyCH4III | ACNGT | 9 cut(s) | 410, 646, 992, 1022, 1196, 1255, 1402, 1916, 2011 |
| HpyCH4IV | ACGT | 1 cut(s) | 2154 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 151, 411, 924, 1277, 2099, 2273, 2498, 2747 |
| HpyF3I | CTNAG | 9 cut(s) | 221, 532, 691, 863, 943, 1458, 1792, 1829, 2184 |
| HpySE526I | ACGT | 1 cut(s) | 2154 |
| HspAI | GCGC | 1 cut(s) | 1858 |
| KpnI | GGTACC | 2 cut(s) | 1302, 2304 |
| LmnI | GCTCC | 4 cut(s) | 81, 573, 1268, 2680 |
| Lsp1109I | GCAGC | 8 cut(s) | 443, 464, 509, 563, 1872, 2102, 2705, 2765 |
| MabI | ACCWGGT | 1 cut(s) | 998 |
| MaeI | CTAG | 8 cut(s) | 1082, 1415, 1475, 1541, 1815, 1959, 2022, 2232 |
| MaeII | ACGT | 1 cut(s) | 2154 |
| MaeIII | GTNAC | 9 cut(s) | 225, 250, 434, 482, 737, 1002, 1910, 1947, 2119 |
| MboII | GAAGA | 9 cut(s) | 162, 165, 580, 1163, 1543, 2076, 2202, 2476, 2804 |
| MfeI | CAATTG | 2 cut(s) | 1420, 1709 |
| MflI | RGATCY | 4 cut(s) | 1141, 1260, 1533, 2203 |
| MhlI | GDGCHC | 2 cut(s) | 1273, 2647 |
| MlsI | TGGCCA | 1 cut(s) | 777 |
| MluNI | TGGCCA | 1 cut(s) | 777 |
| MlyI | GAGTC | 2 cut(s) | 610, 2069 |
| MmeI | TCCRAC | 3 cut(s) | 59, 867, 1155 |
| Mox20I | TGGCCA | 1 cut(s) | 777 |
| Mph1103I | ATGCAT | 2 cut(s) | 816, 1347 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2600 |
| MscI | TGGCCA | 1 cut(s) | 777 |
| MslI | CAYNNNNRTG | 3 cut(s) | 825, 1807, 1838 |
| Msp20I | TGGCCA | 1 cut(s) | 777 |
| MspA1I | CMGCKG | 1 cut(s) | 980 |
| MspCI | CTTAAG | 1 cut(s) | 553 |
| MspR9I | CCNGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| MunI | CAATTG | 2 cut(s) | 1420, 1709 |
| Mva1269I | GAATGC | 2 cut(s) | 695, 2459 |
| MvaI | CCWGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 151, 411, 924, 1277, 2099, 2273, 2498, 2747 |
| NcoI | CCATGG | 1 cut(s) | 1839 |
| NdeI | CATATG | 2 cut(s) | 2244, 2557 |
| NlaIV | GGNNCC | 3 cut(s) | 860, 1300, 2302 |
| NmuCI | GTSAC | 6 cut(s) | 225, 434, 737, 1002, 1910, 2119 |
| NsiI | ATGCAT | 2 cut(s) | 816, 1347 |
| NspI | RCATGY | 3 cut(s) | 1892, 1989, 2042 |
| NspV | TTCGAA | 1 cut(s) | 2790 |
| OliI | CACNNNNGTG | 1 cut(s) | 1807 |
| PaeR7I | CTCGAG | 1 cut(s) | 2417 |
| PceI | AGGCCT | 2 cut(s) | 948, 2600 |
| PciI | ACATGT | 2 cut(s) | 1888, 1985 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2160 |
| PctI | GAATGC | 2 cut(s) | 695, 2459 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2600 |
| PfeI | GAWTC | 9 cut(s) | 28, 466, 632, 1454, 1485, 1604, 1723, 1748, 2723 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1295 |
| PkrI | GCNGC | 9 cut(s) | 458, 479, 499, 578, 1862, 2092, 2695, 2752, 2755 |
| PleI | GAGTC | 2 cut(s) | 610, 2068 |
| PpsI | GAGTC | 2 cut(s) | 610, 2068 |
| PscI | ACATGT | 2 cut(s) | 1888, 1985 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2126 |
| PshBI | ATTAAT | 1 cut(s) | 1340 |
| Psp124BI | GAGCTC | 1 cut(s) | 2647 |
| Psp6I | CCWGG | 4 cut(s) | 619, 998, 2055, 2256 |
| PspGI | CCWGG | 4 cut(s) | 619, 998, 2055, 2256 |
| PspN4I | GGNNCC | 3 cut(s) | 860, 1300, 2302 |
| PspPI | GGNCC | 1 cut(s) | 51 |
| PspXI | VCTCGAGB | 1 cut(s) | 2417 |
| PstI | CTGCAG | 5 cut(s) | 461, 482, 499, 581, 2278 |
| PsuI | RGATCY | 4 cut(s) | 1141, 1260, 1533, 2203 |
| RseI | CAYNNNNRTG | 3 cut(s) | 825, 1807, 1838 |
| SacI | GAGCTC | 1 cut(s) | 2647 |
| SatI | GCNGC | 9 cut(s) | 457, 478, 498, 577, 1861, 2091, 2694, 2751, 2754 |
| Sau96I | GGNCC | 1 cut(s) | 51 |
| ScaI | AGTACT | 2 cut(s) | 1685, 2308 |
| SchI | GAGTC | 2 cut(s) | 610, 2069 |
| ScrFI | CCNGG | 4 cut(s) | 621, 1000, 2057, 2258 |
| SduI | GDGCHC | 2 cut(s) | 1273, 2647 |
| SexAI | ACCWGGT | 1 cut(s) | 998 |
| SfcI | CTRYAG | 6 cut(s) | 457, 478, 495, 577, 1466, 2274 |
| Sfr274I | CTCGAG | 1 cut(s) | 2417 |
| SfuI | TTCGAA | 1 cut(s) | 2790 |
| SinI | GGWCC | 1 cut(s) | 51 |
| SlaI | CTCGAG | 1 cut(s) | 2417 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 825, 1807, 1838 |
| SmlI | CTYRAG | 2 cut(s) | 553, 2417 |
| SmoI | CTYRAG | 2 cut(s) | 553, 2417 |
| SseBI | AGGCCT | 2 cut(s) | 948, 2600 |
| SsiI | CCGC | 4 cut(s) | 980, 1720, 2387, 2750 |
| SspI | AATATT | 2 cut(s) | 505, 2005 |
| SspMI | CTAG | 8 cut(s) | 1082, 1415, 1475, 1541, 1815, 1959, 2022, 2232 |
| SstI | GAGCTC | 1 cut(s) | 2647 |
| StuI | AGGCCT | 2 cut(s) | 948, 2600 |
| StyD4I | CCNGG | 4 cut(s) | 619, 998, 2055, 2256 |
| StyI | CCWWGG | 2 cut(s) | 1839, 2502 |
| TaaI | ACNGT | 9 cut(s) | 410, 646, 992, 1022, 1196, 1255, 1402, 1916, 2011 |
| TaiI | ACGT | 1 cut(s) | 2157 |
| TaqI | TCGA | 7 cut(s) | 388, 1318, 1995, 2163, 2322, 2418, 2790 |
| TatI | WGTACW | 6 cut(s) | 281, 334, 344, 641, 1683, 2306 |
| TauI | GCSGC | 1 cut(s) | 2753 |
| TfiI | GAWTC | 9 cut(s) | 28, 466, 632, 1454, 1485, 1604, 1723, 1748, 2723 |
| TscAI | CASTG | 5 cut(s) | 466, 859, 1405, 1708, 2488 |
| TseFI | GTSAC | 6 cut(s) | 225, 434, 737, 1002, 1910, 2119 |
| TseI | GCWGC | 8 cut(s) | 456, 477, 497, 576, 1860, 2090, 2693, 2753 |
| Tsp45I | GTSAC | 6 cut(s) | 225, 434, 737, 1002, 1910, 2119 |
| TspDTI | ATGAA | 5 cut(s) | 1205, 1248, 1382, 1477, 1544 |
| TspGWI | ACGGA | 4 cut(s) | 1073, 1466, 2236, 2693 |
| TspRI | CASTG | 5 cut(s) | 466, 859, 1405, 1708, 2488 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1295 |
| Vha464I | CTTAAG | 1 cut(s) | 553 |
| VpaK11BI | GGWCC | 1 cut(s) | 51 |
| VspI | ATTAAT | 1 cut(s) | 1340 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 1962, 2324, 2417 |
| XapI | RAATTY | 5 cut(s) | 1304, 1369, 2025, 2330, 2577 |
| XbaI | TCTAGA | 2 cut(s) | 1474, 1814 |
| XceI | RCATGY | 3 cut(s) | 1892, 1989, 2042 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 508, 738, 906 |
| XhoI | CTCGAG | 1 cut(s) | 2417 |
| XmiI | GTMKAC | 2 cut(s) | 1755, 1885 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2600 |
| XspI | CTAG | 8 cut(s) | 1082, 1415, 1475, 1541, 1815, 1959, 2022, 2232 |
| ZrmI | AGTACT | 2 cut(s) | 1685, 2308 |
| Zsp2I | ATGCAT | 2 cut(s) | 816, 1347 |