Rh5DG490000

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001222
Physical Location & Seq
Forward (+)
76991980 .. 77002489
10510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG490000.1

Sequence Viewer

Length: 2910 bp
ATGAGATATCTATGGGAGGAGCTGGTGTTGGAGGTCATTGATGATAACTTAAGCATCACCGCGGATGAACTTTTTCGGGTGTTAGAAGTGTCAGGATGCTTGTCAAGACGATTCCAGGTAGAGTTCGAGTCAGCTCAATGTGTCTTAGATGCTGATATGAATTCTAAATCATATACCACACACTCTCTTGCTATTGTACCTGTTGGCAACACATTGGATAGTAATTTGTTAGTTGATCATGATGATTTTATGAAGAATGTATTGGACTGTAGGGCTGGGATTGCTGATGAACAAAGTGAAGGTTTGTCGATCATTGAAAGGACCTCCCGACAAAACTTCAATGGAACTTCTTCTGCCAAATCTGATATTAGCAGTGATGGAATTTGTGTTACTAAATCCAAAGGCAGAAGAACAGCAAATTGGCTACCTGTGGAACTTCATAAAGAGTCTTGCCCTAGTGCTGTTGATAAGTACTTGGGTAAGGGACGGCTGCCTTCAGGGGAGTTACAAACAGCTGTTAGGAAGCATCTTTTATATTTGGGTTGGAAAGTAGAGTATATGATAGATAAGGGCAGAATTAGATACCGGTACTTGTCGCCTGATGGAGAACCCGAATACTCTCTTATTAAAGTGTGCAGTAACTTGAGAAAGTCCTCGAAAGATTCACAATTTGCAATCTCTCAAGATGCACCTCAGTGTTTGCATGGTTCACCTGGACAGCTTCTTGTCACTGAAGAATCACAAGAGATTCAGCATCCTAATTATTGTCCTCAACAGGTAGCGTCCCCTTTTTCCAATTCCAAGTTACATCCTGAGGTATTTATTTACAAACCCGAATATTGCCCTCAAGCAGTTGTGGCATATGTAGACTCTCCCATAAGGGGACCCAGAAGTTTGAGAGTAAAAACAATGCGGTCTAAAGCAAAGAAGCACCTCTCTGCTGTGGGGTGGGAGTTCTACTACTCAGAAATAAGATCATCAAATAGCTTGCGCTTCAAATCTCCCAAAGGAAATGTATACAACTCACTTCTGGATGCCTGCAAAGTCTGTATGGATGAAGAAAATTCTGAAAGAAGACCTGCTGAATGCAGGTATGTCATTGACGAAGATGAGGCCCACTTAATTAAGAAAAAAATATTTTCTGTAGCAAATAGAAAATGCAAAAGGAATAGAATTCGTAGCTTGTTAAACAGTGGACCTCACTTGCTTCACGAATGCCCAAAGGATCAATGTGCTAACCCTCCAAAGTTGAAAAGGCGAAAGGCATCTGCATCTTCCTCTGGGTTTAGAAATGGTTCTGGTGTTAGTCAGCCAACTCGTGTGCTCAGGTCAAGCAAACGAGTCCAGGAAGTGGTTACTCCTACTTCCACACACCAGAATCCCCGCACTGTCTTATCTTGGTTGATAGACAATAATGTGGTTTTGCCAAGGGAAAAAGTACATTATTGTAGCAGAAAGGGTGGTCCTTCAATGGCAGAAGGGAAAATAAGTAGAGAGGGGATCAAGTGCAGCTGTTGCCAAAAGGTGTTTACTCTTAGCTGCTTTGAGAGTCATGCTGGCTTCAATGAGACTCATGCTGGCTGCAGTAATCACAGACCTGCTTCCAATATTTTTCTGGATGATGGTAGGTCTCTCTTGGATTGCCAGATGCAAATTATACTTGAAAGAAGGAAGAGGACTTGTAGAACAGAACCACGCCATAGGATGAAGGGAAATCGAGTTCGAGGCGAGAGTGATTACATATGTACTGTGTGTCACTATGGTGGTGATTTAATCTTATGTGACGAATGTCCGTCATCATTTCACAAGAGTTGCGTTGGGTTGAAGGATGTTCCAGATGGTGAGTGGTTCTGTCCATCTTGCTGTTGTGGATTATGTGGCCAAAGAAAACTCAAAGAAGACAAAGAACCTATTATGCATTCTGATTTTCTCACATGTGGTCAATGCGAGCACAAATATCACACTGGGTGCCTGAGAAAAGGAGGTGTGGATATGTCGGAAAGTGATTCAAAAGGAAATTGGTTTTGCAGCAAGAATTGTAAAAAGATATCTTTGGGCCTCCACAAGCTTTTAGGGAAACAATTTCCAGCGGGTGTTGGCAATTTAACTTGGTCACTTCTGAAGTCCATGAAATCTGATAGTGATGATGCTACTGAACCTGACAATGATGCCATAACGGAGAGTTTTAGCAGGCTTAGTATTGCCCTTGATGTGATGCACGAGTGTTTTGAGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGCAGAAGATATCATCTTCAGTAGAGGGTCAAATCTCAATCGCTTGAACTTTCAAGGGTTCTATACCGTGCTATTGGAAAGAAATGATGAGCTAATCACTGTGGCTACTGTAAGGATCTACGGAAAGAAGGTGGCCGAAGTACCTCTTGTTGCTACCAGATTTCAATATCGTCGACTTGGAATGTGTCGTGTGTTGATGAATTTACTTGAAAAGATGCTTATGGATTTAGGTGTGGAGAGATTGGTTTTGCCCGCTGTCCCCAGTGTGCTAAACACATGGACTACTGCATTTGGTTTTTCAAGAATGACAGAGTCTGAGAGGCTACAGTTTCTGGATCATACTTTCCTGGATTTTCAGGATACCATTATGTGTCAGAAACTTTTAATGAAGATCTCTCCAGCTGAACCAAGCCTGTTGAAAGGAACACAGCCTAATATTTCTGGAAGTGGTGATAATATTGATCTTGATGGGTCCAGTTCTGTCTCTGAAGTGTGTCAACTGGAAAAAACAGAGGATAGCCAAACCGTATTCCAGGGACTTGAAAGTGCTGCAGGGAAAAGGAGAGCATGCGTTGATAAGAACTGTCCAGAGTTCAAGCAGTACAAGCGAATGAGGAAATCAAATTTGGCTGCCATAGTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

969

Amino Acids

109.1

Weight (kDa)

8.6

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 144 - 218 4.6e-21 Domain of unknown function (DUF7028)
DUF7028 PF22970 276 - 354 1.1e-23 Domain of unknown function (DUF7028)
TDBD PF16135 494 - 551 8.4e-18 Tify domain binding domain
PHD-1st_NSD PF23011 579 - 621 5.3e-07 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 582 - 621 3.7e-08 PHD-finger
IDM1_C PF23209 743 - 881 4.4e-55 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1080, 1087, 1606
AccB1I GGYRCC 1 cut(s) 1968
AccB7I CCANNNNNTGG 1 cut(s) 1351
AccI GTMKAC 3 cut(s) 867, 1017, 2437
AccII CGCG 1 cut(s) 62
AciI CCGC 6 cut(s) 60, 62, 913, 1384, 2090, 2517
AclWI GGATC 4 cut(s) 1233, 1508, 2387, 2607
AcoI YGGCCR 2 cut(s) 1879, 2397
AcsI RAATTY 6 cut(s) 160, 381, 1063, 1173, 2464, 2887
AcuI CTGAAG 5 cut(s) 480, 753, 2141, 2266, 2772
AdeI CACNNNGTG 1 cut(s) 1968
AfaI GTAC 7 cut(s) 198, 473, 590, 1440, 1747, 2406, 2867
AfiI CCNNNNNNNGG 2 cut(s) 881, 1351
AflII CTTAAG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 1934
AgeI ACCGGT 1 cut(s) 585
AjnI CCWGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
AjuI GAANNNNNNNTTGG 6 cut(s) 245, 277, 403, 435, 2873, 2905
AleI CACNNNNGTG 2 cut(s) 694, 1761
AloI GAACNNNNNNTCC 2 cut(s) 1704, 1736
Alw21I GWGCWC 2 cut(s) 1326, 1953
Alw26I GTCTC 3 cut(s) 1562, 1635, 2752
AlwI GGATC 4 cut(s) 1233, 1508, 2387, 2607
AlwNI CAGNNNCTG 2 cut(s) 2579, 2596
AoxI GGCC 4 cut(s) 1113, 1879, 2056, 2397
ApeKI GCWGC 7 cut(s) 490, 1509, 1539, 1581, 2028, 2813, 2894
ApoI RAATTY 6 cut(s) 160, 381, 1063, 1173, 2464, 2887
AsiGI ACCGGT 1 cut(s) 585
Asp700I GAANNNNTTC 3 cut(s) 72, 349, 1294
AspLEI GCGC 1 cut(s) 993
AspS9I GGNCC 7 cut(s) 321, 884, 1114, 1196, 1463, 2056, 2736
AsuHPI GGTGA 5 cut(s) 49, 702, 1778, 1853, 2726
AvaII GGWCC 5 cut(s) 321, 884, 1196, 1463, 2736
AxyI CCTNAGG 1 cut(s) 813
BalI TGGCCA 1 cut(s) 1881
BanI GGYRCC 1 cut(s) 1968
BauI CACGAG 2 cut(s) 1317, 2219
BbsI GAAGAC 2 cut(s) 1081, 1905
Bbv12I GWGCWC 2 cut(s) 1326, 1953
BbvI GCAGC 7 cut(s) 477, 1521, 1526, 1568, 2040, 2800, 2881
BccI CCATC 6 cut(s) 371, 596, 1616, 1832, 1864, 2726
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
BciVI GTATCC 1 cut(s) 2617
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 3 cut(s) 1562, 1635, 2752
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 5 cut(s) 268, 1143, 1582, 2588, 2814
BfrI CTTAAG 1 cut(s) 49
BfuAI ACCTGC 3 cut(s) 1080, 1087, 1606
BfuI GTATCC 1 cut(s) 2617
BglII AGATCT 2 cut(s) 2260, 2655
BisI GCNGC 7 cut(s) 491, 1510, 1540, 1582, 2029, 2814, 2895
BlsI GCNGC 7 cut(s) 492, 1511, 1541, 1583, 2030, 2815, 2896
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
Bme18I GGWCC 5 cut(s) 321, 884, 1196, 1463, 2736
BmgT120I GGNCC 7 cut(s) 321, 884, 1114, 1196, 1463, 2056, 2736
BmiI GGNNCC 5 cut(s) 885, 886, 1970, 2244, 2737
BmrFI CCNGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
BmrI ACTGGG 2 cut(s) 1974, 2520
BmuI ACTGGG 2 cut(s) 1974, 2520
BoxI GACNNNNGTC 1 cut(s) 1788
BpiI GAAGAC 2 cut(s) 1081, 1905
BpmI CTGGAG 1 cut(s) 2646
Bpu10I CCTNAGC 1 cut(s) 1325
BpuEI CTTGAG 3 cut(s) 664, 666, 831
BsaI GGTCTC 1 cut(s) 1635
BsaJI CCNNGG 3 cut(s) 60, 1427, 2797
BsaWI WCCGGW 1 cut(s) 585
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 881, 1351
Bse118I RCCGGY 1 cut(s) 585
Bse1I ACTGG 4 cut(s) 1969, 2526, 2739, 2769
Bse21I CCTNAGG 1 cut(s) 813
BseBI CCWGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
BseDI CCNNGG 3 cut(s) 60, 1427, 2797
BseGI GGATG 9 cut(s) 70, 101, 754, 808, 1039, 1060, 1624, 1710, 1834
BseLI CCNNNNNNNGG 2 cut(s) 881, 1351
BseMII CTCAG 6 cut(s) 707, 804, 978, 1339, 1964, 2571
BseNI ACTGG 4 cut(s) 1969, 2526, 2739, 2769
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 7 cut(s) 477, 1521, 1526, 1568, 2040, 2800, 2881
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 2 cut(s) 655, 1528
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 4 cut(s) 1115, 1881, 2058, 2399
BshNI GGYRCC 1 cut(s) 1968
BshTI ACCGGT 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 1326, 1953
BsiSI CCGG 1 cut(s) 586
BslFI GGGAC 5 cut(s) 498, 769, 897, 2507, 2814
BslI CCNNNNNNNGG 2 cut(s) 881, 1351
BsmAI GTCTC 3 cut(s) 1562, 1635, 2752
BsmFI GGGAC 5 cut(s) 498, 769, 897, 2507, 2814
BsmI GAATGC 3 cut(s) 1091, 1220, 1918
BsnI GGCC 4 cut(s) 1115, 1881, 2058, 2399
Bso31I GGTCTC 1 cut(s) 1635
Bsp1286I GDGCHC 2 cut(s) 1326, 1953
BspACI CCGC 6 cut(s) 60, 62, 913, 1384, 2090, 2517
BspANI GGCC 4 cut(s) 1115, 1881, 2058, 2399
BspCNI CTCAG 6 cut(s) 706, 805, 977, 1338, 1965, 2572
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 5 cut(s) 885, 886, 1970, 2244, 2737
BspMAI CTGCAG 2 cut(s) 1586, 2818
BspMI ACCTGC 3 cut(s) 1080, 1087, 1606
BspPI GGATC 4 cut(s) 1233, 1508, 2387, 2607
BspT107I GGYRCC 1 cut(s) 1968
BspTI CTTAAG 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 1635
BsrFI RCCGGY 1 cut(s) 585
BsrI ACTGG 4 cut(s) 1969, 2526, 2739, 2769
BssAI RCCGGY 1 cut(s) 585
BssECI CCNNGG 3 cut(s) 60, 1427, 2797
BssNAI GTATAC 1 cut(s) 1018
BssSI CACGAG 2 cut(s) 1317, 2219
BssT1I CCWWGG 1 cut(s) 1427
Bst1107I GTATAC 1 cut(s) 1018
Bst2BI CACGAG 2 cut(s) 1317, 2219
Bst2UI CCWGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
Bst6I CTCTTC 1 cut(s) 1667
BstAFI CTTAAG 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 1515
BstC8I GCNNGC 8 cut(s) 989, 1039, 1558, 1579, 1949, 2192, 2517, 2833
BstDEI CTNAG 9 cut(s) 145, 693, 813, 964, 1325, 1535, 1973, 2195, 2580
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 9 cut(s) 70, 101, 754, 808, 1039, 1060, 1624, 1710, 1834
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 993
BstMAI GTCTC 3 cut(s) 1562, 1635, 2752
BstMWI GCNNNNNNNGC 4 cut(s) 281, 857, 1515, 2869
BstNI CCWGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
BstNSI RCATGY 2 cut(s) 1938, 2835
BstPAI GACNNNNGTC 1 cut(s) 1788
BstSCI CCNGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
BstSFI CTRYAG 5 cut(s) 268, 1143, 1582, 2588, 2814
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 7 cut(s) 477, 1521, 1526, 1568, 2040, 2800, 2881
BstV2I GAAGAC 2 cut(s) 1081, 1905
BstX2I RGATCY 3 cut(s) 2260, 2379, 2655
BstYI RGATCY 3 cut(s) 2260, 2379, 2655
BstZ17I GTATAC 1 cut(s) 1018
Bsu36I CCTNAGG 1 cut(s) 813
BsuI GTATCC 1 cut(s) 2617
BsuRI GGCC 4 cut(s) 1115, 1881, 2058, 2399
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 9 cut(s) 70, 101, 754, 808, 1039, 1060, 1624, 1710, 1834
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 8 cut(s) 379, 701, 729, 1198, 1386, 1962, 2361, 2533
BveI ACCTGC 3 cut(s) 1080, 1087, 1606
Cac8I GCNNGC 8 cut(s) 989, 1039, 1558, 1579, 1949, 2192, 2517, 2833
CaiI CAGNNNCTG 2 cut(s) 2579, 2596
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 993
Cfr10I RCCGGY 1 cut(s) 585
Cfr13I GGNCC 7 cut(s) 321, 884, 1114, 1196, 1463, 2056, 2736
Cfr42I CCGCGG 1 cut(s) 63
CseI GACGC 1 cut(s) 771
Csp6I GTAC 7 cut(s) 197, 472, 589, 1439, 1746, 2405, 2866
CspAI ACCGGT 1 cut(s) 585
CviAII CATG 8 cut(s) 239, 704, 1553, 1574, 1935, 2128, 2541, 2832
CviQI GTAC 7 cut(s) 197, 472, 589, 1439, 1746, 2405, 2866
DdeI CTNAG 9 cut(s) 145, 693, 813, 964, 1325, 1535, 1973, 2195, 2580
DraIII CACNNNGTG 1 cut(s) 1968
EaeI YGGCCR 2 cut(s) 1879, 2397
Eam1104I CTCTTC 1 cut(s) 1667
EarI CTCTTC 1 cut(s) 1667
Eco130I CCWWGG 1 cut(s) 1427
Eco31I GGTCTC 1 cut(s) 1635
Eco32I GATATC 3 cut(s) 8, 2049, 2275
Eco47I GGWCC 5 cut(s) 321, 884, 1196, 1463, 2736
Eco57I CTGAAG 5 cut(s) 480, 753, 2141, 2266, 2772
Eco81I CCTNAGG 1 cut(s) 813
EcoO109I RGGNCCY 2 cut(s) 321, 884
EcoRI GAATTC 2 cut(s) 160, 1173
EcoRII CCWGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
EcoRV GATATC 3 cut(s) 8, 2049, 2275
EcoT14I CCWWGG 1 cut(s) 1427
EcoT22I ATGCAT 1 cut(s) 1920
ErhI CCWWGG 1 cut(s) 1427
FaeI CATG 8 cut(s) 242, 707, 1556, 1577, 1938, 2131, 2544, 2835
FalI AAGNNNNNCTT 2 cut(s) 2394, 2426
FaqI GGGAC 5 cut(s) 498, 769, 897, 2507, 2814
FatI CATG 8 cut(s) 238, 703, 1552, 1573, 1934, 2127, 2540, 2831
FauI CCCGC 3 cut(s) 1391, 2083, 2524
FauNDI CATATG 2 cut(s) 862, 1742
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 3 cut(s) 867, 1017, 2437
Fnu4HI GCNGC 7 cut(s) 491, 1510, 1540, 1582, 2029, 2814, 2895
FokI GGATG 9 cut(s) 77, 108, 741, 795, 1046, 1067, 1631, 1717, 1841
Fsp4HI GCNGC 7 cut(s) 491, 1510, 1540, 1582, 2029, 2814, 2895
FspBI CTAG 1 cut(s) 456
GlaI GCGC 1 cut(s) 992
GluI GCNGC 7 cut(s) 491, 1510, 1540, 1582, 2029, 2814, 2895
GsaI CCCAGC 1 cut(s) 279
GsuI CTGGAG 1 cut(s) 2646
HaeIII GGCC 4 cut(s) 1115, 1881, 2058, 2399
HapII CCGG 1 cut(s) 586
HgaI GACGC 1 cut(s) 771
HhaI GCGC 1 cut(s) 993
Hin1II CATG 8 cut(s) 242, 707, 1556, 1577, 1938, 2131, 2544, 2835
Hin6I GCGC 1 cut(s) 991
HinP1I GCGC 1 cut(s) 991
HincII GTYRAC 2 cut(s) 2438, 2762
HindII GTYRAC 2 cut(s) 2438, 2762
HindIII AAGCTT 1 cut(s) 2066
HpaII CCGG 1 cut(s) 586
HphI GGTGA 5 cut(s) 49, 702, 1778, 1853, 2726
Hpy166II GTNNAC 7 cut(s) 710, 868, 1018, 1196, 1530, 2438, 2762
Hpy8I GTNNAC 7 cut(s) 710, 868, 1018, 1196, 1530, 2438, 2762
Hpy99I CGWCG 1 cut(s) 2439
HpyAV CCTTC 8 cut(s) 293, 504, 1472, 1476, 1662, 1702, 1819, 2386
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 857, 1515, 2869
HpyF3I CTNAG 9 cut(s) 145, 693, 813, 964, 1325, 1535, 1973, 2195, 2580
Hsp92II CATG 8 cut(s) 242, 707, 1556, 1577, 1938, 2131, 2544, 2835
HspAI GCGC 1 cut(s) 991
KflI GGGWCCC 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 235
KspI CCGCGG 1 cut(s) 63
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 7 cut(s) 477, 1521, 1526, 1568, 2040, 2800, 2881
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 9 cut(s) 388, 504, 638, 727, 804, 1354, 1754, 1781, 2112
MflI RGATCY 3 cut(s) 2260, 2379, 2655
MhlI GDGCHC 2 cut(s) 1326, 1953
MlsI TGGCCA 1 cut(s) 1881
MluNI TGGCCA 1 cut(s) 1881
MlyI GAGTC 7 cut(s) 137, 455, 863, 1350, 1558, 1564, 2585
MmeI TCCRAC 3 cut(s) 9, 524, 1977
Mox20I TGGCCA 1 cut(s) 1881
Mph1103I ATGCAT 1 cut(s) 1920
MroXI GAANNNNTTC 3 cut(s) 72, 349, 1294
MscI TGGCCA 1 cut(s) 1881
MseI TTAA 9 cut(s) 50, 627, 1121, 1125, 1187, 1772, 2105, 2238, 2648
MslI CAYNNNNRTG 2 cut(s) 694, 1761
Msp20I TGGCCA 1 cut(s) 1881
MspA1I CMGCKG 6 cut(s) 62, 515, 1512, 2090, 2519, 2666
MspCI CTTAAG 1 cut(s) 49
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
Mva1269I GAATGC 3 cut(s) 1091, 1220, 1918
MvaI CCWGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 4 cut(s) 281, 857, 1515, 2869
NdeI CATATG 2 cut(s) 862, 1742
NlaIII CATG 8 cut(s) 242, 707, 1556, 1577, 1938, 2131, 2544, 2835
NlaIV GGNNCC 5 cut(s) 885, 886, 1970, 2244, 2737
NmuCI GTSAC 4 cut(s) 727, 1754, 1781, 2112
NsiI ATGCAT 1 cut(s) 1920
NspI RCATGY 2 cut(s) 1938, 2835
OliI CACNNNNGTG 2 cut(s) 694, 1761
PacI TTAATTAA 1 cut(s) 1125
PaeI GCATGC 1 cut(s) 2835
PagI TCATGA 1 cut(s) 238
PciI ACATGT 1 cut(s) 1934
PctI GAATGC 3 cut(s) 1091, 1220, 1918
PdmI GAANNNNTTC 3 cut(s) 72, 349, 1294
PfeI GAWTC 6 cut(s) 111, 662, 737, 748, 1378, 2006
PflFI GACNNNGTC 1 cut(s) 2575
PflMI CCANNNNNTGG 1 cut(s) 1351
PfoI TCCNGGA 2 cut(s) 1344, 2610
PinAI ACCGGT 1 cut(s) 585
PkrI GCNGC 7 cut(s) 492, 1511, 1541, 1583, 2030, 2815, 2896
PleI GAGTC 7 cut(s) 136, 454, 863, 1349, 1557, 1564, 2584
PpsI GAGTC 7 cut(s) 136, 454, 863, 1349, 1557, 1564, 2584
PpuMI RGGWCCY 2 cut(s) 321, 884
PscI ACATGT 1 cut(s) 1934
PshAI GACNNNNGTC 1 cut(s) 1788
Psp5II RGGWCCY 2 cut(s) 321, 884
Psp6I CCWGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
PspN4I GGNNCC 5 cut(s) 885, 886, 1970, 2244, 2737
PspPI GGNCC 7 cut(s) 321, 884, 1114, 1196, 1463, 2056, 2736
PspPPI RGGWCCY 2 cut(s) 321, 884
PstI CTGCAG 2 cut(s) 1586, 2818
PstNI CAGNNNCTG 2 cut(s) 2579, 2596
PsuI RGATCY 3 cut(s) 2260, 2379, 2655
PsyI GACNNNGTC 1 cut(s) 2575
PvuII CAGCTG 3 cut(s) 515, 1512, 2666
RsaI GTAC 7 cut(s) 198, 473, 590, 1440, 1747, 2406, 2867
RsaNI GTAC 7 cut(s) 197, 472, 589, 1439, 1746, 2405, 2866
RseI CAYNNNNRTG 2 cut(s) 694, 1761
SacII CCGCGG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 2436
SaqAI TTAA 9 cut(s) 50, 627, 1121, 1125, 1187, 1772, 2105, 2238, 2648
SatI GCNGC 7 cut(s) 491, 1510, 1540, 1582, 2029, 2814, 2895
Sau96I GGNCC 7 cut(s) 321, 884, 1114, 1196, 1463, 2056, 2736
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 7 cut(s) 137, 455, 863, 1350, 1558, 1564, 2585
ScrFI CCNGG 6 cut(s) 116, 714, 1346, 2254, 2612, 2798
SduI GDGCHC 2 cut(s) 1326, 1953
SfcI CTRYAG 5 cut(s) 268, 1143, 1582, 2588, 2814
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SinI GGWCC 5 cut(s) 321, 884, 1196, 1463, 2736
SmiMI CAYNNNNRTG 2 cut(s) 694, 1761
SmlI CTYRAG 4 cut(s) 49, 643, 681, 846
SmoI CTYRAG 4 cut(s) 49, 643, 681, 846
SphI GCATGC 1 cut(s) 2835
SsiI CCGC 6 cut(s) 60, 62, 913, 1384, 2090, 2517
SspI AATATT 5 cut(s) 839, 1137, 1609, 2701, 2722
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 6 cut(s) 114, 712, 1344, 2252, 2610, 2796
StyI CCWWGG 1 cut(s) 1427
TaqI TCGA 6 cut(s) 126, 308, 656, 1717, 1723, 2437
TatI WGTACW 4 cut(s) 471, 1438, 1745, 2865
TfiI GAWTC 6 cut(s) 111, 662, 737, 748, 1378, 2006
Tru1I TTAA 9 cut(s) 50, 627, 1121, 1125, 1187, 1772, 2105, 2238, 2648
Tru9I TTAA 9 cut(s) 50, 627, 1121, 1125, 1187, 1772, 2105, 2238, 2648
TscAI CASTG 8 cut(s) 379, 701, 736, 1198, 1393, 1969, 2368, 2533
TseFI GTSAC 4 cut(s) 727, 1754, 1781, 2112
TseI GCWGC 7 cut(s) 490, 1509, 1539, 1581, 2028, 2813, 2894
Tsp45I GTSAC 4 cut(s) 727, 1754, 1781, 2112
TspGWI ACGGA 3 cut(s) 1782, 2192, 2400
TspRI CASTG 8 cut(s) 379, 701, 736, 1198, 1393, 1969, 2368, 2533
Tth111I GACNNNGTC 1 cut(s) 2575
Van91I CCANNNNNTGG 1 cut(s) 1351
Vha464I CTTAAG 1 cut(s) 49
VpaK11BI GGWCC 5 cut(s) 321, 884, 1196, 1463, 2736
XapI RAATTY 6 cut(s) 160, 381, 1063, 1173, 2464, 2887
XceI RCATGY 2 cut(s) 1938, 2835
XcmI CCANNNNNNNNNTGG 2 cut(s) 1612, 1842
XmiI GTMKAC 3 cut(s) 867, 1017, 2437
XmnI GAANNNNTTC 3 cut(s) 72, 349, 1294
XspI CTAG 1 cut(s) 456
ZrmI AGTACT 1 cut(s) 473
Zsp2I ATGCAT 1 cut(s) 1920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.