pycom11g05960

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001222
Physical Location & Seq
Reverse (-)
4583028 .. 4588975
5948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g05960.6

Sequence Viewer

Length: 3921 bp
ATGGCGGTCATGGAGCTCGAAGAGCCCAAAAAACGAAAACGCAGGACTCACAATCGACGAAAGCTTCTCGTTGGTGCCCAAGTTGAGGTGAGGAGTGTAGAAGCTGGGTTTCAGGGGTCATGGCACCCTGGGATTGTCATTGCATGCAATAAGCAAGGTCATCGTCAAGTTCAATACGAACATCTTCTATGTGACGATGAGTCTGGTAATCTGGTTGATGTTGTGTCTGTTTCACCCATTTTGGATGGCATTGGTTCATTCACTGGGAACGGGAGTAACTATCGCGGGTGTATAAGGCCAACGCCACCTCACATTCAGCCAGGGGAATGGGACCTTCCCTATGGACTCTGTGTGGATGTGTTTCATGAGGACGGTTGGTGGGAAGGTGTCATCTTCGATCATGAAGATGGTTCGGAGGAGAGAAGGATCTTCTTCCCGGATTTGGGTGATGAATTAAATGCTCGGATTGACACTATCCGAATTACTCACGACTGGGATGAGGTTACTGAGAATTGGAAGCGACGCGGTACTTGGGTGTTGCTTGAGTTGCTTGAGAAATATGATAACGAGAGGTACATTGCTGTTTCTGTGAAGCAAATTTGGTATGATGTTCGAGAAAAAAAGGGATTTGAGAATGTCAAGGAGTGGACTTCTCCAATGAGACATCTGTGGGAAGAGTTGGTGTTGGAGGTCATTGATGATAACATAAGCATCACAGTTGATGAACTATTAGGTGTTTTAGACAAGTCAGGATGCTTGTCACTAGAAACCCAGGTAGAATTGGAGTCAGCTCATTTTTCCTCTGATGCTAATATGAATCCTAAAGAAAATATGGTTGACTCTTCAGCTATTGTACCTGTCCACAACCCGCTGTATAGAGATCCGTTAGTTGGTCATGAAGATACTTTCAAGAATGTAATTAACTGTGTACTGAACTGCAATGCTGAGTTTATTGATGAACAAGATGTAGATGGGGGTTTGTCTATTAATCCAGACTCTGCCTCCGCGCAGCAGGTTCACAAGAAATCTTATATGGCTAAATTGATCTCTGCGGCAGGGGCTGACATACCAGATATGAATAGTTTGGAGTGCTCAAATATATTTTTCCAAAATAAAGGAGTGTCTGTTCTACCTCAGGTTTTGTCAGCATTTCCTTCTAATCTTGATGGGAATTCTTGTGTCAATTCTGGTATCAGAAATGGTGGAATTCGTAGTTTTAATTCCAGAAGCAAAAGTACCCTTAATTGGCTACCTTTCGACCCTCAAGCTATATATTGCCCTGACTCTGTTGATGAATATTTGAATAAGCGTTCTAATTTCAAAGTGACAGCTGTTAGGCAGTATCTTCAATATTTGGGGTGGAAAATGGAATTTGCAATTGATAAGGGCAACTACAGATACCGGTACTTGTGTCCTGACAGAGGAGAATACGAGTACTCTCTTTTTCAAGTGTGTAAGAAGTTGAAGAAACCCAAGAGAGATACACTTTCAATCTCTGAAGATGCAACCCAGGGTTTGCATTGTTCAGAAGAACAGACTCTTCTCATTGAACAACCACAAGAGAGTCATCATCCTAGTTATTGTCCTCAGATGTCAGGGTCTTCTTACCACGAAGAATTTATTTACAAACCTGAATATTGCCACGAAGCAGTTGTGGAATATTATAACCATGCAATGGCCAAGGGAACGAAGAAGAAAGTACGAAAAATGGTATCAAAAGCAAAGAAGCACCTCTCAGCTGTTGGGTGGGTGTTCTTGTACGCAAGTAGAAATTCAAAGAACTTGTGCTACAAATCACCCAAAGGAGTGGTGCATTTTACACTTTATAGTGCATGCAAAAGCTGTATGGATGGAGATTTTGTAGAGAGACCTGCTGAATGCATGTATGTCATTGAGGAAGACGAGGGCCAGTCCACTAGAAACAGAATATGCTCTACAGCAAGTAATTTGGTTAATCATGAAGGCTGGGTACCATCGAAAACTGTGTCCAAAAATTGGTCTAGAGATACTATTAGCATGTCACAGGCAAGTAATCTTGTGGAGATTGGTAATGTGAAAGTTCATGGTAGTAGCAAATGCCCAAAAAAGAGAAAATACGGACCAACAGAGTTAATAGGTGATGAAAAACTAAGATGTCTAAAGGATCAATGTGCCAACCCTCCAAAGTTCAAAAGGAGAAGGGGATCTGGAGCTTTGGATGGGTTAAGAGATGAATTGGGTGGTAGTCAGCCAACTCGTGTGCTCAGGTCAAGCAAACGGGTGCAGGAAGCAACCTCCTCACACAAGAATCCTCGCAATGTCTTATCTTGGTTGATAGACAATAACATGGTTTCTCCAAGGGAAAAAGTAAATTATCGGAGCACAAAGGATAGTCATCCAATGAAACAAGGGAAGATAACTCGTGAGTCGATCAGGTGCAGCTGTTGCACAGAGGTGTATACTCTTAGTTCCTTTGGGCATCACGCTGGCAGTAGTAACCACAGACCTTATGCCAATATTTTCTTGGAAGATGGTAGGTCTCTCTTAGATTGCCAGGTGCAAATTATGCATGAAAGAAGGGAGAGGAACTTCCGAAAACAACCACGTGATAGGATGAAGGGCAATTGGTGCCGAGGTGAGAATGATTACATTTGCACTGTGTGTCACTTTGGTGGTGACCTAATTTTATGTGATCAATGTCCATCATCATTTCACAAAAAATGCCTTGGCTTGAAGGCTGTTCCAGACGGTGACTGGTTCTGTTCATCTTGCCGTTGTGGAATTTGTGGCCAAAAGAACTTTAAAGAAGAGAAAGAGTCTGTTGTGGATGATAATTATGTTCTCACCTGTGGTCAATGCGAGCATAAATATCACAAAGGGTGCCTGAGAAAAAGAGGCGCAGAAGAAAACAAAAGTGATCCAGAAGGAAACTGGTATTGCAGCAAAAACTGTAAAAAGATATCTTTCAACCTAGACGAGCGTTTAGGAAAACAAATTCCAGTGGGTGATGACAATCTATCTTGGTCACTGTTGAAGTTCAACAAGTGTGATACCCATGCTACCGATGAACATGATACTGATGATGCCTTGACGGAGTGTTATAGCAAGCTCAATGTTGCTCTTGATGTGATGCATGAGTGTTTTGTGCCTGTTAAGGAACCTCTTACCAGGAGAGATCTTGTAGAAGATGTCATCTTCAGTAGAGGGTCAGATCTTAATCGCCTGAACTTCCGAGGGTTCTATACTGTGCTTTTGGAGAGAAATGATGAATTAATCACTGCGGCTACTGTAAGGATTTTCGGAGACAAAGTGGCAGAAGTACCTCTTGTTGCTACCAGATTTCAATATCGTCGGCTTGGAATGTGTCGTATATTAATGGATGAGCTTGAAAAGATGCTTATGGAATTAGGTGTGGAGAGATTGGTTTTACCCGCCGTACCAAGCGTGCTAAACACATGGACAACATCATTTGGGTTCTCAAAGATGACACCTTCCGAGAGGCTAAAGTTTCTGGATTATACTTTCCTTGATTTTCAGGGTACCATTATGTGTCAGAAACTGTTAAACAAGAATGCTCCAGCTGAAGCAAGGCCATTAAAAAGAACCGTGCTTGATCGTTACGGTGATATCTGTGGAAGTCGTGATAATACTGACGTTGATATGTCCGTTACCGTCTCTGAAGTGTATCAAGCAGAACCAATTGAGGATGGCAGAATTGCGTGCCAAGGAGTAGAAAACGCTGCAGGTAAACAAAGTAGACCATGCCCAGGTGAGAGCAATCCAGTATTCTCCTCCTTCGAGGATGCTGACTGCAACAAAGTATCTCGTAAGTACTACAAGCAGTACAAGCGGAGGAGGATATCAACAAAAGGAATCCAAGTTATGCTAAATCACCTCACGCTACATGATCAAAATAAATGTGGTCAGCTGTGCAGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003690 GO:0003712 GO:0003714 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030334 GO:0030336 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032879 GO:0032991 GO:0033558 GO:0035064 GO:0035601 GO:0036211 GO:0040012 GO:0040013 GO:0042393 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051270 GO:0051271 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070577 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140030 GO:0140033 GO:0140034 GO:0140096 GO:0140097 GO:0140110 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1903756 GO:1903758 GO:1904949 GO:1990837 GO:2000112 GO:2000113 GO:2000145 GO:2000146 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Agenet Jas PHD

Protein Analysis

1307

Amino Acids

148.77

Weight (kDa)

6.95

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1665
Acc36I ACCTGC 3 cut(s) 1003, 1879, 3713
Acc65I GGTACC 2 cut(s) 1969, 3518
AccB1I GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3518
AccB7I CCANNNNNTGG 2 cut(s) 1675, 1995
AccI GTMKAC 2 cut(s) 2438, 3736
AccII CGCG 3 cut(s) 285, 525, 1007
AciI CCGC 9 cut(s) 5, 285, 525, 869, 1005, 1052, 3260, 3411, 3829
AclWI GGATC 5 cut(s) 434, 875, 2151, 2191, 2891
AcoI YGGCCR 2 cut(s) 1677, 2767
AcsI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 1771, 2760, 2973
AcuI CTGAAG 5 cut(s) 828, 1518, 3160, 3582, 3678
AcvI CACGTG 1 cut(s) 2585
AdeI CACNNNGTG 1 cut(s) 2640
AfiI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3746
AgeI ACCGGT 1 cut(s) 1401
AhdI GACNNNNNGTC 1 cut(s) 199
AjnI CCWGG 7 cut(s) 127, 319, 771, 1509, 2532, 3146, 3745
AleI CACNNNNGTG 2 cut(s) 2432, 2649
AloI GAACNNNNNNTCC 2 cut(s) 1110, 1142
Alw21I GWGCWC 4 cut(s) 18, 1094, 2244, 2363
Alw26I GTCTC 5 cut(s) 655, 1861, 2523, 3276, 3658
AlwI GGATC 5 cut(s) 434, 875, 2151, 2191, 2891
AlwNI CAGNNNCTG 3 cut(s) 998, 1061, 3538
AoxI GGCC 5 cut(s) 296, 1677, 1906, 2767, 3569
ApeKI GCWGC 4 cut(s) 1009, 2418, 2919, 3719
ApoI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 1771, 2760, 2973
ArsI GACNNNNNNTTYG 2 cut(s) 1970, 2002
AseI ATTAAT 3 cut(s) 987, 3251, 3353
AsiGI ACCGGT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 183
Asp718I GGTACC 2 cut(s) 1969, 3518
AspLEI GCGC 2 cut(s) 1009, 2879
AspS9I GGNCC 3 cut(s) 331, 1906, 2099
AsuC2I CCSGG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 331, 2099
AxyI CCTNAGG 1 cut(s) 1134
BaeGI GKGCMC 1 cut(s) 79
BaeI ACNNNNGTAYC 4 cut(s) 519, 552, 1396, 1429
BalI TGGCCA 2 cut(s) 1679, 2769
BanI GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3518
BanII GRGCYC 2 cut(s) 18, 27
BarI GAAGNNNNNNTAC 4 cut(s) 1219, 1251, 1953, 1985
BauI CACGAG 2 cut(s) 2235, 2400
BbrPI CACGTG 1 cut(s) 2585
BbsI GAAGAC 2 cut(s) 1593, 1905
Bbv12I GWGCWC 4 cut(s) 18, 1094, 2244, 2363
BbvI GCAGC 4 cut(s) 1021, 2430, 2931, 3706
BceAI ACGGC 2 cut(s) 2736, 3398
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 7 cut(s) 129, 321, 773, 1511, 2534, 3148, 3747
BclI TGATCA 2 cut(s) 2671, 3886
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 5 cut(s) 655, 1861, 2523, 3276, 3658
BfaI CTAG 6 cut(s) 764, 1575, 1917, 2001, 2951, 3919
BfmI CTRYAG 3 cut(s) 1393, 1935, 3720
BfuAI ACCTGC 3 cut(s) 1003, 1879, 3713
BglII AGATCT 2 cut(s) 3154, 3190
BisI GCNGC 6 cut(s) 1010, 1053, 2419, 2920, 3261, 3720
BlsI GCNGC 6 cut(s) 1011, 1054, 2420, 2921, 3262, 3721
BmcAI AGTACT 2 cut(s) 1436, 3812
Bme1390I CCNGG 8 cut(s) 129, 321, 437, 773, 1511, 2534, 3148, 3747
Bme18I GGWCC 2 cut(s) 331, 2099
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgT120I GGNCC 3 cut(s) 331, 1906, 2099
BmiI GGNNCC 8 cut(s) 76, 125, 332, 1971, 2609, 2861, 3138, 3520
BmrFI CCNGG 8 cut(s) 129, 321, 437, 773, 1511, 2534, 3148, 3747
BmrI ACTGGG 2 cut(s) 273, 502
BmsI GCATC 9 cut(s) 720, 743, 796, 1492, 2467, 3052, 3099, 3363, 3772
BmuI ACTGGG 2 cut(s) 273, 502
BpiI GAAGAC 2 cut(s) 1593, 1905
BpmI CTGGAG 2 cut(s) 2208, 3540
Bpu10I CCTNAGC 1 cut(s) 2243
BpuEI CTTGAG 3 cut(s) 563, 572, 1248
BpuMI CCSGG 1 cut(s) 437
BsaAI YACGTR 1 cut(s) 2585
BsaBI GATNNNNATC 3 cut(s) 2373, 2991, 3195
BsaI GGTCTC 2 cut(s) 1861, 2523
BsaWI WCCGGW 1 cut(s) 1401
BsaXI ACNNNNNCTCC 8 cut(s) 986, 1016, 1110, 1140, 1845, 1875, 3273, 3303
Bsc4I CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3746
Bse118I RCCGGY 1 cut(s) 1401
Bse1I ACTGG 7 cut(s) 268, 497, 1909, 2738, 2915, 2978, 3761
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
Bse8I GATNNNNATC 3 cut(s) 2373, 2991, 3195
BseBI CCWGG 7 cut(s) 129, 321, 773, 1511, 2534, 3148, 3747
BseJI GATNNNNATC 3 cut(s) 2373, 2991, 3195
BseLI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3746
BseMI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
BseMII CTCAG 7 cut(s) 498, 936, 1148, 1601, 1749, 2257, 2855
BseNI ACTGG 7 cut(s) 268, 497, 1909, 2738, 2915, 2978, 3761
BseRI GAGGAG 6 cut(s) 106, 431, 1437, 2266, 3760, 3847
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 4 cut(s) 1021, 2430, 2931, 3706
BseYI CCCAGC 2 cut(s) 104, 1965
BsgI GTGCAG 2 cut(s) 2282, 2437
Bsh1236I CGCG 3 cut(s) 285, 525, 1007
BshFI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3571
BshNI GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3518
BshTI ACCGGT 1 cut(s) 1401
BsiHKAI GWGCWC 4 cut(s) 18, 1094, 2244, 2363
BsiSI CCGG 2 cut(s) 437, 1402
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 9 cut(s) 85, 442, 443, 890, 1245, 1421, 1675, 1995, 3746
BsmAI GTCTC 5 cut(s) 655, 1861, 2523, 3276, 3658
BsmBI CGTCTC 1 cut(s) 3658
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 2 cut(s) 1883, 3556
BsnI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3571
Bso31I GGTCTC 2 cut(s) 1861, 2523
Bsp1286I GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
BspACI CCGC 9 cut(s) 5, 285, 525, 869, 1005, 1052, 3260, 3411, 3829
BspANI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3571
BspCNI CTCAG 7 cut(s) 499, 937, 1147, 1600, 1748, 2256, 2856
BspFNI CGCG 3 cut(s) 285, 525, 1007
BspHI TCATGA 4 cut(s) 364, 400, 895, 1957
BspLI GGNNCC 8 cut(s) 76, 125, 332, 1971, 2609, 2861, 3138, 3520
BspMAI CTGCAG 1 cut(s) 3724
BspMI ACCTGC 3 cut(s) 1003, 1879, 3713
BspPI GGATC 5 cut(s) 434, 875, 2151, 2191, 2891
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 6 cut(s) 74, 123, 1969, 2607, 2859, 3518
BspTNI GGTCTC 2 cut(s) 1861, 2523
BsrDI GCAATG 5 cut(s) 138, 576, 946, 1680, 2302
BsrFI RCCGGY 1 cut(s) 1401
BsrI ACTGG 7 cut(s) 268, 497, 1909, 2738, 2915, 2978, 3761
BssAI RCCGGY 1 cut(s) 1401
BssNAI GTATAC 1 cut(s) 2439
BssSI CACGAG 2 cut(s) 2235, 2400
BssT1I CCWWGG 4 cut(s) 1680, 2336, 2704, 3703
Bst1107I GTATAC 1 cut(s) 2439
Bst2BI CACGAG 2 cut(s) 2235, 2400
Bst2UI CCWGG 7 cut(s) 129, 321, 773, 1511, 2534, 3148, 3747
Bst6I CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
BstAPI GCANNNNNTGC 3 cut(s) 2424, 2545, 2607
BstBAI YACGTR 1 cut(s) 2585
BstC8I GCNNGC 7 cut(s) 145, 1834, 2467, 2840, 3086, 3425, 3700
BstEII GGTNACC 1 cut(s) 2654
BstENI CCTNNNNNAGG 1 cut(s) 1419
BstFNI CGCG 3 cut(s) 285, 525, 1007
BstHHI GCGC 2 cut(s) 1009, 2879
BstMAI GTCTC 5 cut(s) 655, 1861, 2523, 3276, 3658
BstMWI GCNNNNNNNGC 7 cut(s) 22, 547, 1058, 2424, 2545, 2607, 3826
BstNI CCWGG 7 cut(s) 129, 321, 773, 1511, 2534, 3148, 3747
BstNSI RCATGY 4 cut(s) 147, 1836, 1885, 2020
BstPI GGTNACC 1 cut(s) 2654
BstSCI CCNGG 8 cut(s) 127, 319, 435, 771, 1509, 2532, 3146, 3745
BstSFI CTRYAG 3 cut(s) 1393, 1935, 3720
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 3 cut(s) 285, 525, 1007
BstV1I GCAGC 4 cut(s) 1021, 2430, 2931, 3706
BstV2I GAAGAC 2 cut(s) 1593, 1905
BstX2I RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
BstXI CCANNNNNNTGG 2 cut(s) 327, 1807
BstYI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
BstZ17I GTATAC 1 cut(s) 2439
Bsu36I CCTNAGG 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 298, 1679, 1908, 2769, 3571
BtsI GCAGTG 2 cut(s) 3255, 3919
BtsIMutI CAGTG 6 cut(s) 261, 2634, 2985, 3005, 3255, 3919
BveI ACCTGC 3 cut(s) 1003, 1879, 3713
Cac8I GCNNGC 7 cut(s) 145, 1834, 2467, 2840, 3086, 3425, 3700
CaiI CAGNNNCTG 3 cut(s) 998, 1061, 3538
CciI TCATGA 4 cut(s) 364, 400, 895, 1957
CfoI GCGC 2 cut(s) 1009, 2879
Cfr10I RCCGGY 1 cut(s) 1401
Cfr13I GGNCC 3 cut(s) 331, 1906, 2099
CseI GACGC 1 cut(s) 531
CspAI ACCGGT 1 cut(s) 1401
DraI TTTAAA 1 cut(s) 2782
DraIII CACNNNGTG 1 cut(s) 2640
DriI GACNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 2 cut(s) 1677, 2767
Eam1104I CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 5 cut(s) 15, 669, 847, 1545, 2781
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 4 cut(s) 1680, 2336, 2704, 3703
Eco24I GRGCYC 2 cut(s) 18, 27
Eco31I GGTCTC 2 cut(s) 1861, 2523
Eco32I GATATC 3 cut(s) 2940, 3607, 3840
Eco47I GGWCC 2 cut(s) 331, 2099
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 5 cut(s) 828, 1518, 3160, 3582, 3678
Eco72I CACGTG 1 cut(s) 2585
Eco81I CCTNAGG 1 cut(s) 1134
Eco91I GGTNACC 1 cut(s) 2654
EcoICRI GAGCTC 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 1419
EcoO109I RGGNCCY 1 cut(s) 331
EcoO65I GGTNACC 1 cut(s) 2654
EcoRI GAATTC 2 cut(s) 1171, 1206
EcoRII CCWGG 7 cut(s) 127, 319, 771, 1509, 2532, 3146, 3745
EcoRV GATATC 3 cut(s) 2940, 3607, 3840
EcoT14I CCWWGG 4 cut(s) 1680, 2336, 2704, 3703
EcoT22I ATGCAT 3 cut(s) 1883, 2550, 3114
EcoT38I GRGCYC 2 cut(s) 18, 27
ErhI CCWWGG 4 cut(s) 1680, 2336, 2704, 3703
Esp3I CGTCTC 1 cut(s) 3658
FalI AAGNNNNNCTT 2 cut(s) 3288, 3320
FaqI GGGAC 1 cut(s) 344
FauI CCCGC 3 cut(s) 278, 876, 3418
FbaI TGATCA 2 cut(s) 2671, 3886
FblI GTMKAC 2 cut(s) 2438, 3736
Fnu4HI GCNGC 6 cut(s) 1010, 1053, 2419, 2920, 3261, 3720
FriOI GRGCYC 2 cut(s) 18, 27
Fsp4HI GCNGC 6 cut(s) 1010, 1053, 2419, 2920, 3261, 3720
FspBI CTAG 6 cut(s) 764, 1575, 1917, 2001, 2951, 3919
GlaI GCGC 2 cut(s) 1008, 2878
GluI GCNGC 6 cut(s) 1010, 1053, 2419, 2920, 3261, 3720
GsaI CCCAGC 2 cut(s) 108, 1969
GsuI CTGGAG 2 cut(s) 2208, 3540
HaeIII GGCC 5 cut(s) 298, 1679, 1908, 2769, 3571
HapII CCGG 2 cut(s) 437, 1402
HgaI GACGC 1 cut(s) 531
HhaI GCGC 2 cut(s) 1009, 2879
Hin6I GCGC 2 cut(s) 1007, 2877
HinP1I GCGC 2 cut(s) 1007, 2877
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HindIII AAGCTT 1 cut(s) 62
HpaII CCGG 2 cut(s) 437, 1402
Hpy166II GTNNAC 9 cut(s) 648, 838, 862, 929, 1018, 1914, 2439, 3728, 3737
Hpy8I GTNNAC 9 cut(s) 648, 838, 862, 929, 1018, 1914, 2439, 3728, 3737
Hpy99I CGWCG 3 cut(s) 60, 525, 3333
HpyCH4IV ACGT 2 cut(s) 2584, 3633
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 547, 1058, 2424, 2545, 2607, 3826
HpySE526I ACGT 2 cut(s) 2584, 3633
HspAI GCGC 2 cut(s) 1007, 2877
KpnI GGTACC 2 cut(s) 1973, 3522
Ksp22I TGATCA 2 cut(s) 2671, 3886
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 4 cut(s) 13, 2189, 2358, 3559
Lsp1109I GCAGC 4 cut(s) 1021, 2430, 2931, 3706
LweI GCATC 9 cut(s) 720, 743, 796, 1492, 2467, 3052, 3099, 3363, 3772
MaeI CTAG 6 cut(s) 764, 1575, 1917, 2001, 2951, 3919
MaeII ACGT 2 cut(s) 2584, 3633
MfeI CAATTG 3 cut(s) 1377, 2602, 3678
MflI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
MhlI GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
MlsI TGGCCA 2 cut(s) 1679, 2769
MluNI TGGCCA 2 cut(s) 1679, 2769
MmeI TCCRAC 1 cut(s) 666
Mox20I TGGCCA 2 cut(s) 1679, 2769
Mph1103I ATGCAT 3 cut(s) 1883, 2550, 3114
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 2 cut(s) 1679, 2769
MslI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
Msp20I TGGCCA 2 cut(s) 1679, 2769
MspA1I CMGCKG 6 cut(s) 871, 1331, 1739, 2421, 3560, 3907
MspI CCGG 2 cut(s) 437, 1402
MspR9I CCNGG 8 cut(s) 129, 321, 437, 773, 1511, 2534, 3148, 3747
MunI CAATTG 3 cut(s) 1377, 2602, 3678
Mva1269I GAATGC 2 cut(s) 1883, 3556
MvaI CCWGG 7 cut(s) 129, 321, 773, 1511, 2534, 3148, 3747
MvnI CGCG 3 cut(s) 285, 525, 1007
MwoI GCNNNNNNNGC 7 cut(s) 22, 547, 1058, 2424, 2545, 2607, 3826
NciI CCSGG 1 cut(s) 437
NlaIV GGNNCC 8 cut(s) 76, 125, 332, 1971, 2609, 2861, 3138, 3520
NmeAIII GCCGAG 1 cut(s) 2636
NmuCI GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
NsiI ATGCAT 3 cut(s) 1883, 2550, 3114
NspI RCATGY 4 cut(s) 147, 1836, 1885, 2020
OliI CACNNNNGTG 2 cut(s) 2432, 2649
PaeI GCATGC 2 cut(s) 147, 1836
PagI TCATGA 4 cut(s) 364, 400, 895, 1957
PasI CCCWGGG 2 cut(s) 128, 1510
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 2 cut(s) 1883, 3556
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 817, 2287, 3852
PflMI CCANNNNNTGG 2 cut(s) 1675, 1995
PfoI TCCNGGA 1 cut(s) 435
PinAI ACCGGT 1 cut(s) 1401
PkrI GCNGC 6 cut(s) 1011, 1054, 2420, 2921, 3262, 3721
PmaCI CACGTG 1 cut(s) 2585
PmlI CACGTG 1 cut(s) 2585
Ppu21I YACGTR 1 cut(s) 2585
PpuMI RGGWCCY 1 cut(s) 331
PshBI ATTAAT 3 cut(s) 987, 3251, 3353
PsiI TTATAA 1 cut(s) 1665
Psp124BI GAGCTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 7 cut(s) 127, 319, 771, 1509, 2532, 3146, 3745
PspCI CACGTG 1 cut(s) 2585
PspEI GGTNACC 1 cut(s) 2654
PspFI CCCAGC 2 cut(s) 104, 1965
PspGI CCWGG 7 cut(s) 127, 319, 771, 1509, 2532, 3146, 3745
PspN4I GGNNCC 8 cut(s) 76, 125, 332, 1971, 2609, 2861, 3138, 3520
PspPI GGNCC 3 cut(s) 331, 1906, 2099
PspPPI RGGWCCY 1 cut(s) 331
PsrI GAACNNNNNNTAC 2 cut(s) 1772, 1804
PstI CTGCAG 1 cut(s) 3724
PstNI CAGNNNCTG 3 cut(s) 998, 1061, 3538
PsuI RGATCY 5 cut(s) 426, 880, 2183, 3154, 3190
PvuII CAGCTG 5 cut(s) 1331, 1739, 2421, 3560, 3907
RseI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
SacI GAGCTC 1 cut(s) 18
SapI GCTCTTC 1 cut(s) 15
SatI GCNGC 6 cut(s) 1010, 1053, 2419, 2920, 3261, 3720
Sau96I GGNCC 3 cut(s) 331, 1906, 2099
ScaI AGTACT 2 cut(s) 1436, 3812
ScrFI CCNGG 8 cut(s) 129, 321, 437, 773, 1511, 2534, 3148, 3747
SduI GDGCHC 6 cut(s) 18, 27, 79, 1094, 2244, 2363
SfaNI GCATC 9 cut(s) 720, 743, 796, 1492, 2467, 3052, 3099, 3363, 3772
SfcI CTRYAG 3 cut(s) 1393, 1935, 3720
SinI GGWCC 2 cut(s) 331, 2099
SmiMI CAYNNNNRTG 3 cut(s) 405, 2432, 2649
SmlI CTYRAG 3 cut(s) 542, 551, 1263
SmoI CTYRAG 3 cut(s) 542, 551, 1263
SphI GCATGC 2 cut(s) 147, 1836
SsiI CCGC 9 cut(s) 5, 285, 525, 869, 1005, 1052, 3260, 3411, 3829
SspI AATATT 5 cut(s) 1298, 1352, 1637, 1661, 2497
SspMI CTAG 6 cut(s) 764, 1575, 1917, 2001, 2951, 3919
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 8 cut(s) 127, 319, 435, 771, 1509, 2532, 3146, 3745
StyI CCWWGG 4 cut(s) 1680, 2336, 2704, 3703
TaiI ACGT 2 cut(s) 2587, 3636
TaqI TCGA 8 cut(s) 18, 55, 396, 613, 1257, 1976, 2408, 3777
TatI WGTACW 4 cut(s) 928, 1434, 3810, 3822
TauI GCSGC 2 cut(s) 1055, 3263
TfiI GAWTC 3 cut(s) 817, 2287, 3852
TscAI CASTG 6 cut(s) 268, 2641, 2985, 3012, 3262, 3919
TseFI GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
TseI GCWGC 4 cut(s) 1009, 2418, 2919, 3719
Tsp45I GTSAC 8 cut(s) 191, 759, 1324, 2019, 2642, 2654, 2729, 3003
TspGWI ACGGA 4 cut(s) 873, 2112, 3086, 3634
TspRI CASTG 6 cut(s) 268, 2641, 2985, 3012, 3262, 3919
Van91I CCANNNNNTGG 2 cut(s) 1675, 1995
VpaK11BI GGWCC 2 cut(s) 331, 2099
VspI ATTAAT 3 cut(s) 987, 3251, 3353
XagI CCTNNNNNAGG 1 cut(s) 1419
XapI RAATTY 8 cut(s) 597, 1171, 1206, 1370, 1616, 1771, 2760, 2973
XbaI TCTAGA 1 cut(s) 2000
XceI RCATGY 4 cut(s) 147, 1836, 1885, 2020
XcmI CCANNNNNNNNNTGG 3 cut(s) 2500, 2730, 2907
XmiI GTMKAC 2 cut(s) 2438, 3736
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 6 cut(s) 764, 1575, 1917, 2001, 2951, 3919
ZrmI AGTACT 2 cut(s) 1436, 3812
Zsp2I ATGCAT 3 cut(s) 1883, 2550, 3114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.