MD11G1163800.v1.1
ERF Family

BEST Arabidopsis thaliana protein match is alpha beta-Hydrolases superfamily protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001884
Physical Location & Seq
Forward (+)
16637055 .. 16640286
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1163800.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGATTGGCCCAGTAGCAATAGCTTTGACTGTGGGTCTTCTTGGATGGGCTTATCAGGCACTAAAGCCTCCCCATCCCAAAATATGTGGATCGCCGGACGGTCCTCCTGTCACTTCTCCTAGAGTAAAACTCAGTGATGGGAGGCATTTGGCTTACAGGGAATTGGGAGTTCCAAAAGAAGAGGCGAAACACAAGATCATCGTCATTCATGGCTTTTTAAGTTCCAAAGATATGAGTTTGCCCGTCACTCAAGAACTCATAGAGGAGTTAAGGATATATTTCCTGTTCTTTGACAGAGCTGGCTATGGAGAGAGTGATCCATATCCTTCACGTTCTGTGAAGAGCGAAGCTTTTGATATTCAAGAGCTAGCTGACAAGTTGCTGATTGGGTCTAAATTTTATGTAATCGGAATCTCAATGGGAGCTTACCCTGTTTGGAGTTGCTTGAAATACATACCACACAGATTGTCTGGAGCTTCCCTGGTAGTTCCCTTTGTGAACTACTGGTGGCCTCGTTTTCCTGCTGATCTATCAAGAGAGTCCCTTGGTACGCTGCAGCTATCAGACCAATGGACATTCCGAGTTGCACATTACACTCCTTGGTTATTCCATTGGTGGATGACCCAGAAATGGTTCCCTTCACTAAGTATCTTGGCTGGGAATAACGCAATTTTCTGCCCGAAAGATGTAGAGATGTTGCAGAAACTCTCAGAAACACCAAGTCTTGGTCAGGAAAAGATTACGCAGCAAGGAGAGCACGAATCACTGTTCCGAGACATACTGGCTGGTTATGGAAAATGGGAATTCGACCCCTTGGATCTCAGCAATCCCTTCCCTGACAACGAGGGATCAGTTCACATTTGGCAAGGCTATGAGGACAGGATCATTCCTTACAAACTAAACCGTCATATTGCAGAGAAGCTTCCCTGGATTCATTACCATGAAGTTCCTGATTTTGGGCATTTTCTGATGTTCGACAAGAACCAATGTGAAGCCGTTTTGAAGGCACTTTTGCTTGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

342

Amino Acids

39.0

Weight (kDa)

6.23

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 61 - 327 9e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 178 5.3e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 326 3.3e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 725
AclWI GGATC 5 cut(s) 97, 311, 825, 856, 890
AcsI RAATTY 2 cut(s) 395, 803
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 630, 725, 956
AgsI TTSAA 3 cut(s) 362, 448, 1003
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 2 cut(s) 480, 926
AluBI AGCT 9 cut(s) 23, 299, 350, 367, 371, 425, 476, 559, 922
AluI AGCT 9 cut(s) 23, 299, 350, 367, 371, 425, 476, 559, 922
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 5 cut(s) 97, 311, 825, 856, 890
AoxI GGCC 2 cut(s) 7, 509
ApeKI GCWGC 3 cut(s) 553, 556, 745
ApoI RAATTY 2 cut(s) 395, 803
Asp700I GAANNNNTTC 1 cut(s) 632
AspS9I GGNCC 2 cut(s) 8, 101
AsuNHI GCTAGC 1 cut(s) 367
AvaII GGWCC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 3 cut(s) 540, 568, 757
BccI CCATC 3 cut(s) 39, 81, 131
BceAI ACGGC 1 cut(s) 980
BciT130I CCWGG 2 cut(s) 482, 928
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 2 cut(s) 120, 368
BfmI CTRYAG 1 cut(s) 554
BisI GCNGC 3 cut(s) 554, 557, 746
BlsI GCNGC 3 cut(s) 555, 558, 747
Bme1390I CCNGG 2 cut(s) 482, 928
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 33
BmgT120I GGNCC 2 cut(s) 8, 101
BmiI GGNNCC 1 cut(s) 635
BmrFI CCNGG 2 cut(s) 482, 928
BmrI ACTGGG 1 cut(s) 5
BmtI GCTAGC 1 cut(s) 371
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 1 cut(s) 29
BplI GAGNNNNNCTC 2 cut(s) 114, 146
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 5 cut(s) 480, 544, 599, 813, 926
Bsc4I CCNNNNNNNGG 3 cut(s) 630, 725, 956
Bse1I ACTGG 3 cut(s) 11, 509, 786
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 482, 928
BseDI CCNNGG 5 cut(s) 480, 544, 599, 813, 926
BseGI GGATG 3 cut(s) 50, 73, 624
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 3 cut(s) 630, 725, 956
BseMII CTCAG 3 cut(s) 145, 723, 835
BseNI ACTGG 3 cut(s) 11, 509, 786
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 540, 568, 757
BseYI CCCAGC 1 cut(s) 656
BshFI GGCC 2 cut(s) 9, 511
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 3 cut(s) 630, 725, 956
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 2 cut(s) 9, 511
Bsp1286I GDGCHC 1 cut(s) 759
Bsp143I GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
BspANI GGCC 2 cut(s) 9, 511
BspCNI CTCAG 3 cut(s) 144, 722, 834
BspLI GGNNCC 1 cut(s) 635
BspMAI CTGCAG 1 cut(s) 558
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 5 cut(s) 97, 311, 825, 856, 890
BspQI GCTCTTC 1 cut(s) 335
BsrI ACTGG 3 cut(s) 11, 509, 786
BssECI CCNNGG 5 cut(s) 480, 544, 599, 813, 926
BssMI GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
BssT1I CCWWGG 3 cut(s) 544, 599, 813
Bst2UI CCWGG 2 cut(s) 482, 928
Bst4CI ACNGT 4 cut(s) 31, 101, 768, 905
Bst6I CTCTTC 2 cut(s) 174, 335
BstC8I GCNNGC 2 cut(s) 301, 369
BstDEI CTNAG 4 cut(s) 131, 644, 709, 821
BstF5I GGATG 3 cut(s) 50, 73, 624
BstKTI GATC 7 cut(s) 92, 198, 319, 529, 820, 851, 885
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
BstMWI GCNNNNNNNGC 2 cut(s) 56, 754
BstNI CCWGG 2 cut(s) 482, 928
BstSCI CCNGG 2 cut(s) 480, 926
BstSFI CTRYAG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 540, 568, 757
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
BsuRI GGCC 2 cut(s) 9, 511
BtsCI GGATG 3 cut(s) 50, 73, 624
BtsIMutI CAGTG 2 cut(s) 139, 764
Cac8I GCNNGC 2 cut(s) 301, 369
Cfr13I GGNCC 2 cut(s) 8, 101
Csp6I GTAC 1 cut(s) 549
CspCI CAANNNNNGTGG 4 cut(s) 67, 102, 447, 482
CviAII CATG 2 cut(s) 209, 941
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 4 cut(s) 131, 644, 709, 821
DpnI GATC 7 cut(s) 91, 197, 318, 528, 819, 850, 884
DpnII GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
DriI GACNNNNNGTC 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 174, 335
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 2 cut(s) 174, 335
Eco130I CCWWGG 3 cut(s) 544, 599, 813
Eco47I GGWCC 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 803
EcoRII CCWGG 2 cut(s) 480, 926
EcoT14I CCWWGG 3 cut(s) 544, 599, 813
ErhI CCWWGG 3 cut(s) 544, 599, 813
FaeI CATG 2 cut(s) 212, 944
FaqI GGGAC 1 cut(s) 526
FatI CATG 2 cut(s) 208, 940
Fnu4HI GCNGC 3 cut(s) 554, 557, 746
FokI GGATG 3 cut(s) 57, 60, 631
Fsp4HI GCNGC 3 cut(s) 554, 557, 746
FspBI CTAG 2 cut(s) 120, 368
GluI GCNGC 3 cut(s) 554, 557, 746
GsaI CCCAGC 1 cut(s) 660
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 2 cut(s) 9, 511
HapII CCGG 1 cut(s) 95
Hin1II CATG 2 cut(s) 212, 944
HindIII AAGCTT 2 cut(s) 348, 920
HinfI GANTC 4 cut(s) 411, 539, 761, 931
HpaII CCGG 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 499, 856
Hpy188I TCNGA 6 cut(s) 410, 565, 581, 712, 773, 969
Hpy188III TCNNGA 6 cut(s) 251, 362, 471, 534, 731, 950
Hpy8I GTNNAC 2 cut(s) 499, 856
HpyAV CCTTC 4 cut(s) 336, 648, 841, 997
HpyCH4III ACNGT 4 cut(s) 31, 101, 768, 905
HpyCH4IV ACGT 1 cut(s) 331
HpyCH4V TGCA 4 cut(s) 556, 587, 700, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 754
HpyF3I CTNAG 4 cut(s) 131, 644, 709, 821
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 2 cut(s) 212, 944
Kzo9I GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 2 cut(s) 422, 473
Lsp1109I GCAGC 3 cut(s) 540, 568, 757
MaeI CTAG 2 cut(s) 120, 368
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 2 cut(s) 109, 244
MalI GATC 7 cut(s) 91, 197, 318, 528, 819, 850, 884
MboI GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
MboII GAAGA 3 cut(s) 29, 191, 352
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 4 cut(s) 161, 395, 669, 803
MlyI GAGTC 1 cut(s) 548
MnlI CCTC 8 cut(s) 78, 114, 135, 175, 256, 522, 838, 868
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 2 cut(s) 218, 269
MslI CAYNNNNRTG 1 cut(s) 939
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 482, 928
MvaI CCWGG 2 cut(s) 482, 928
MwoI GCNNNNNNNGC 2 cut(s) 56, 754
NdeII GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 2 cut(s) 212, 944
NlaIV GGNNCC 1 cut(s) 635
NmuCI GTSAC 2 cut(s) 109, 244
PciSI GCTCTTC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 411, 761, 931
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 3 cut(s) 555, 558, 747
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 480, 926
PspFI CCCAGC 1 cut(s) 656
PspGI CCWGG 2 cut(s) 480, 926
PspN4I GGNNCC 1 cut(s) 635
PspPI GGNCC 2 cut(s) 8, 101
PstI CTGCAG 1 cut(s) 558
PsuI RGATCY 1 cut(s) 817
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 939
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 2 cut(s) 218, 269
SatI GCNGC 3 cut(s) 554, 557, 746
Sau3AI GATC 7 cut(s) 89, 195, 316, 526, 817, 848, 882
Sau96I GGNCC 2 cut(s) 8, 101
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 2 cut(s) 482, 928
SduI GDGCHC 1 cut(s) 759
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 4 cut(s) 161, 395, 669, 803
SspMI CTAG 2 cut(s) 120, 368
StyD4I CCNGG 2 cut(s) 480, 926
StyI CCWWGG 3 cut(s) 544, 599, 813
TaaI ACNGT 4 cut(s) 31, 101, 768, 905
TaiI ACGT 1 cut(s) 334
TaqI TCGA 2 cut(s) 807, 975
TasI AATT 4 cut(s) 161, 395, 669, 803
TfiI GAWTC 3 cut(s) 411, 761, 931
Tru1I TTAA 2 cut(s) 218, 269
Tru9I TTAA 2 cut(s) 218, 269
TscAI CASTG 2 cut(s) 139, 771
TseFI GTSAC 2 cut(s) 109, 244
TseI GCWGC 3 cut(s) 553, 556, 745
Tsp45I GTSAC 2 cut(s) 109, 244
TspDTI ATGAA 3 cut(s) 197, 923, 957
TspRI CASTG 2 cut(s) 139, 771
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 2 cut(s) 395, 803
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 2 cut(s) 120, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.