Rw5G027540
ERF Family

BEST Arabidopsis thaliana protein match is alpha beta-Hydrolases superfamily protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001884
Physical Location & Seq
Forward (+)
40831084 .. 40835373
4290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027540.1

Sequence Viewer

Length: 1023 bp
ATGATTGCCCCAGTAGTACTAGCTTTGACAGTGGGTTTTCTTGGGTGGGCATATCAGGCACTAAAGCCTCCCCCTCCTAAAATATGTGGTTCACCTGGTGGTCCTCCTGTCACTTCACCTAGAGTGAAACTCAGTGATGGTAGATATTTGGCCTACAGGGAGTTTGGAGTGCCTAAAGAAGAGGCAAAACACAAGATCATCATCATTCATGGCTTTAGCAGTTCCAAAGATCTGGCTTTACCTGTTTCTCAAGAACTCGTTGACGAGTTAAGGGTATATTTTCTCTTCTTTGATAGAGCTGGTTATGGCGAAAGTGATCCGTATCCCTCACGCTCGGTTAAGAGTGAAGCATATGATATTCAAGAACTAGCCGACACGTTGCAGATTGGGTCCAAATTCTATGTAATTGGAATCTCAATGGGGGCTTACCCTGTTTGGAGTTGCTTGAAATACATACCGCACAGATTGTTAGGAGCTTCCCTGGTAGTTCCTTTTGTGAACTACTGGTGGCCTCGTATTCCTGCTAATCTATTGAGAGAGTCCTTTCAGAGGCTGCAGGTATCAGACCAACGGACGTTCCAAGTTGCACATTACACCCCATGGTTGCTTCATTGGTGGATGACCCAGAAATGGTTCACTGCATTAAGTATGATGGCTGGAAATATGGAGATCTTCTGCCCCAAAGATTTAGAAACATTGAAGAAACTTTCTGAATCCCCAAGTGTTGGTCAGGAAAAGATACAGCAGCAAGGAGAACACGAATCTCTCTACCGAGACCTACTGGCTGGCTATGGAAAATGGGAATTCGACCCCATAGACTTAACGAATCCATTTCCTAACAATGAGGGTTCAGTTCACATTTGGCAAGGCTATGCAGACAGGATCATTCCATATAAAGTAAACCGTTACATTGCAGAGAAGCTTCCCTGGATTCATTATCACGAAGTTCCTGATTATGGGCATTTCCTTATTTTTGAGAGTGATCCATGTGAGGCTGTTTTGAAGGCACTCTTGCGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

340

Amino Acids

38.94

Weight (kDa)

6.56

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 61 - 327 9.3e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 327 7.2e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 66 - 335 8.9e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 547
AccB7I CCANNNNNTGG 1 cut(s) 725
AciI CCGC 1 cut(s) 458
AclWI GGATC 3 cut(s) 311, 890, 977
AcsI RAATTY 2 cut(s) 395, 803
AdeI CACNNNGTG 1 cut(s) 98
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 4 cut(s) 549, 630, 725, 956
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 4 cut(s) 362, 448, 700, 1003
AjnI CCWGG 3 cut(s) 94, 480, 926
AluBI AGCT 4 cut(s) 23, 299, 476, 922
AluI AGCT 4 cut(s) 23, 299, 476, 922
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 3 cut(s) 311, 890, 977
AlwNI CAGNNNCTG 1 cut(s) 553
AoxI GGCC 2 cut(s) 150, 509
ApeKI GCWGC 2 cut(s) 553, 745
ApoI RAATTY 2 cut(s) 395, 803
Asp700I GAANNNNTTC 1 cut(s) 632
AspS9I GGNCC 2 cut(s) 101, 390
AsuHPI GGTGA 2 cut(s) 84, 108
AvaII GGWCC 2 cut(s) 101, 390
BbvI GCAGC 2 cut(s) 540, 757
BccI CCATC 2 cut(s) 131, 646
BciT130I CCWGG 3 cut(s) 96, 482, 928
BciVI GTATCC 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 3 cut(s) 20, 120, 368
BfmI CTRYAG 2 cut(s) 154, 554
BfuAI ACCTGC 1 cut(s) 547
BfuI GTATCC 1 cut(s) 333
BglII AGATCT 2 cut(s) 229, 669
BisI GCNGC 2 cut(s) 554, 746
BlsI GCNGC 2 cut(s) 555, 747
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 3 cut(s) 96, 482, 928
Bme18I GGWCC 2 cut(s) 101, 390
BmgT120I GGNCC 2 cut(s) 101, 390
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 3 cut(s) 96, 482, 928
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BplI GAGNNNNNCTC 2 cut(s) 114, 146
BpuEI CTTGAG 1 cut(s) 234
BsaBI GATNNNNATC 2 cut(s) 200, 321
BsaI GGTCTC 1 cut(s) 768
BsaJI CCNNGG 3 cut(s) 480, 599, 926
Bsc4I CCNNNNNNNGG 4 cut(s) 549, 630, 725, 956
Bse1I ACTGG 3 cut(s) 11, 509, 786
Bse3DI GCAATG 1 cut(s) 909
Bse8I GATNNNNATC 2 cut(s) 200, 321
BseBI CCWGG 3 cut(s) 96, 482, 928
BseDI CCNNGG 3 cut(s) 480, 599, 926
BseGI GGATG 1 cut(s) 624
BseJI GATNNNNATC 2 cut(s) 200, 321
BseLI CCNNNNNNNGG 4 cut(s) 549, 630, 725, 956
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 1 cut(s) 145
BseNI ACTGG 3 cut(s) 11, 509, 786
BseXI GCAGC 2 cut(s) 540, 757
BshFI GGCC 2 cut(s) 152, 511
BslI CCNNNNNNNGG 4 cut(s) 549, 630, 725, 956
BsmAI GTCTC 1 cut(s) 768
BsnI GGCC 2 cut(s) 152, 511
Bso31I GGTCTC 1 cut(s) 768
Bsp143I GATC 6 cut(s) 195, 229, 316, 669, 882, 982
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 1 cut(s) 458
BspANI GGCC 2 cut(s) 152, 511
BspCNI CTCAG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 391
BspMAI CTGCAG 1 cut(s) 558
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 3 cut(s) 311, 890, 977
BspTNI GGTCTC 1 cut(s) 768
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 3 cut(s) 11, 509, 786
BssECI CCNNGG 3 cut(s) 480, 599, 926
BssMI GATC 6 cut(s) 195, 229, 316, 669, 882, 982
BssT1I CCWWGG 1 cut(s) 599
Bst2UI CCWGG 3 cut(s) 96, 482, 928
Bst4CI ACNGT 2 cut(s) 31, 905
Bst6I CTCTTC 2 cut(s) 174, 290
BstC8I GCNNGC 1 cut(s) 787
BstDEI CTNAG 1 cut(s) 131
BstDSI CCRYGG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 547
BstF5I GGATG 1 cut(s) 624
BstKTI GATC 6 cut(s) 198, 232, 319, 672, 885, 985
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 6 cut(s) 195, 229, 316, 669, 882, 982
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 3 cut(s) 96, 482, 928
BstSCI CCNGG 3 cut(s) 94, 480, 926
BstSFI CTRYAG 2 cut(s) 154, 554
BstV1I GCAGC 2 cut(s) 540, 757
BstX2I RGATCY 2 cut(s) 229, 669
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 2 cut(s) 229, 669
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 2 cut(s) 152, 511
BtgI CCRYGG 1 cut(s) 599
BtsCI GGATG 1 cut(s) 624
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 3 cut(s) 36, 139, 636
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 787
CaiI CAGNNNCTG 1 cut(s) 553
Cfr13I GGNCC 2 cut(s) 101, 390
CsiI ACCWGGT 1 cut(s) 94
Csp6I GTAC 1 cut(s) 17
CviAII CATG 3 cut(s) 209, 600, 987
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 1 cut(s) 131
DpnI GATC 6 cut(s) 197, 231, 318, 671, 884, 984
DpnII GATC 6 cut(s) 195, 229, 316, 669, 882, 982
DraIII CACNNNGTG 1 cut(s) 98
Eam1104I CTCTTC 2 cut(s) 174, 290
EarI CTCTTC 2 cut(s) 174, 290
Eco130I CCWWGG 1 cut(s) 599
Eco31I GGTCTC 1 cut(s) 768
Eco47I GGWCC 2 cut(s) 101, 390
EcoNI CCTNNNNNAGG 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 803
EcoRII CCWGG 3 cut(s) 94, 480, 926
EcoT14I CCWWGG 1 cut(s) 599
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 3 cut(s) 212, 603, 990
FalI AAGNNNNNCTT 1 cut(s) 995
FatI CATG 3 cut(s) 208, 599, 986
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 554, 746
FokI GGATG 1 cut(s) 631
Fsp4HI GCNGC 2 cut(s) 554, 746
FspBI CTAG 3 cut(s) 20, 120, 368
GluI GCNGC 2 cut(s) 554, 746
HaeIII GGCC 2 cut(s) 152, 511
Hin1II CATG 3 cut(s) 212, 603, 990
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 6 cut(s) 411, 539, 713, 761, 826, 931
HphI GGTGA 2 cut(s) 84, 108
Hpy166II GTNNAC 6 cut(s) 92, 262, 499, 636, 856, 901
Hpy188I TCNGA 3 cut(s) 549, 565, 712
Hpy188III TCNNGA 5 cut(s) 251, 362, 731, 941, 950
Hpy8I GTNNAC 6 cut(s) 92, 262, 499, 636, 856, 901
HpyAV CCTTC 1 cut(s) 997
HpyCH4III ACNGT 2 cut(s) 31, 905
HpyCH4IV ACGT 2 cut(s) 377, 575
HpyCH4V TGCA 6 cut(s) 382, 556, 587, 641, 875, 914
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 377, 575
Hsp92II CATG 3 cut(s) 212, 603, 990
Kzo9I GATC 6 cut(s) 195, 229, 316, 669, 882, 982
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 2 cut(s) 540, 757
MabI ACCWGGT 1 cut(s) 94
MaeI CTAG 3 cut(s) 20, 120, 368
MaeII ACGT 2 cut(s) 377, 575
MaeIII GTNAC 2 cut(s) 109, 905
MalI GATC 6 cut(s) 197, 231, 318, 671, 884, 984
MboI GATC 6 cut(s) 195, 229, 316, 669, 882, 982
MboII GAAGA 4 cut(s) 191, 277, 664, 712
MflI RGATCY 2 cut(s) 229, 669
MluCI AATT 3 cut(s) 395, 405, 803
MlyI GAGTC 1 cut(s) 548
MnlI CCTC 9 cut(s) 78, 84, 114, 175, 337, 522, 543, 838, 985
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 4 cut(s) 269, 339, 644, 821
MspR9I CCNGG 3 cut(s) 96, 482, 928
MvaI CCWGG 3 cut(s) 96, 482, 928
MwoI GCNNNNNNNGC 1 cut(s) 56
NcoI CCATGG 1 cut(s) 599
NdeI CATATG 1 cut(s) 352
NdeII GATC 6 cut(s) 195, 229, 316, 669, 882, 982
NlaIII CATG 3 cut(s) 212, 603, 990
NlaIV GGNNCC 1 cut(s) 391
NmuCI GTSAC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 5 cut(s) 411, 713, 761, 826, 931
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 2 cut(s) 555, 747
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
Psp6I CCWGG 3 cut(s) 94, 480, 926
PspGI CCWGG 3 cut(s) 94, 480, 926
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 2 cut(s) 101, 390
PstI CTGCAG 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 553
PsuI RGATCY 2 cut(s) 229, 669
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SaqAI TTAA 4 cut(s) 269, 339, 644, 821
SatI GCNGC 2 cut(s) 554, 746
Sau3AI GATC 6 cut(s) 195, 229, 316, 669, 882, 982
Sau96I GGNCC 2 cut(s) 101, 390
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 3 cut(s) 96, 482, 928
SexAI ACCWGGT 1 cut(s) 94
SfcI CTRYAG 2 cut(s) 154, 554
SinI GGWCC 2 cut(s) 101, 390
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 3 cut(s) 395, 405, 803
SsiI CCGC 1 cut(s) 458
SspMI CTAG 3 cut(s) 20, 120, 368
StyD4I CCNGG 3 cut(s) 94, 480, 926
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 2 cut(s) 31, 905
TaiI ACGT 2 cut(s) 380, 578
TaqI TCGA 1 cut(s) 807
TasI AATT 3 cut(s) 395, 405, 803
TatI WGTACW 1 cut(s) 16
TfiI GAWTC 5 cut(s) 411, 713, 761, 826, 931
Tru1I TTAA 4 cut(s) 269, 339, 644, 821
Tru9I TTAA 4 cut(s) 269, 339, 644, 821
TscAI CASTG 3 cut(s) 36, 139, 643
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 553, 745
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 3 cut(s) 197, 599, 923
TspGWI ACGGA 2 cut(s) 309, 586
TspRI CASTG 3 cut(s) 36, 139, 643
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 101, 390
XagI CCTNNNNNAGG 1 cut(s) 547
XapI RAATTY 2 cut(s) 395, 803
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 3 cut(s) 20, 120, 368
ZrmI AGTACT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.