MD11G1222400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002487
Physical Location & Seq
Reverse (-)
32565057 .. 32570024
4968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1222400.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGTGTACAAGAACAAGAACCAGCACAGGCGCGGGTCCTATTTCCAGTACCTTATGAAGGTAAGGAGGGATTTGAGGCTTTTACAATCAGCTAAACTGGAGGAACTTTTGGGTTGCTGTTTCCAAAGTATTACTGGGAAGAGACCTAAGCAAAAGTTGCATTTACTAGAAAGCTTGAAGAGAAGGAAATGTGAAAGTGGAAAGCATAATTTTATGGAGCGACTTCTTGGAGCTGCACGCCTTTTATCACAGATGGTTGAGCCAATGTTGAAGGCGGCTATTGAGATATCTACGTTGCTTGCTCGATCGTTCTTCATGGGATTTTCTCTGACTGTGTTAGCTCTGCTTGCACGACTTCGAGTTTTGGTTCAACAAATTCTACTCGATGCTGTTTCAGTATTCAACATGGTTTCTTCTCTCTCCCAAACACAGCAATCCGTCAAGATAATTCAAGGAGGACTTGAGGTGTTTAGGGAGTTTTACCCTTCAAATGAGGAAATTATCATTCTTGAGTGTGTGTGGAAATCAGATAAGTTTGAATTGATTGAGAGGACACATAAGACTGAGATTGCAACTCATGATGGAGATCTTTGTTTAGGGGAATCATCTGTGCTGTATAAAAGCACCGAGTCTATCCTTGGAGATGATGAAACTATTCCCGAAACGGCAGATGCAAACCACAGTGCTGAAAAAGTTCCAATCGAGGTCAAGGAGGATAAGATCGATTTGATGTCAGGCCTGTCCAACGACGGTGATAACAGTCAACTGGTTGAGCATTGCGAAGATGGTCCAGACATTGGAGAGAATCCGAGCGAGAACTTTTCCGAAGAAGGCAGCTTGCTAACAGGCACAAGCTTGTCTCAAAGTTCCGATGCATCAAAACTGCAGTCTGGACCTGTTAAAGTGGCGTTTATACAAGTGAAACATCCCTTATACTTGACGGCGAATACAACTGAAATCCATTCTAAAGAAAATGACATCAGTAGCAGTACCAAGGAAGACCCATTATTCAGTTTGCTTTCTGGTGGAAATGTAATGAGTAGAGTCTCTTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4477

Protein Analysis

353

Amino Acids

39.29

Weight (kDa)

5.6

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEPRO_N PF14780 2 - 135 1.6e-08 Nucleolus and neural progenitor protein, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 797
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 33, 275
AcsI RAATTY 1 cut(s) 375
AfaI GTAC 3 cut(s) 8, 50, 991
AfiI CCNNNNNNNGG 2 cut(s) 58, 797
AgsI TTSAA 7 cut(s) 178, 271, 371, 403, 452, 489, 539
AluBI AGCT 6 cut(s) 92, 174, 233, 341, 837, 855
AluI AGCT 6 cut(s) 92, 174, 233, 341, 837, 855
Alw26I GTCTC 3 cut(s) 136, 864, 1051
AoxI GGCC 1 cut(s) 736
ApeKI GCWGC 2 cut(s) 233, 834
ApoI RAATTY 1 cut(s) 375
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
Asp700I GAANNNNTTC 2 cut(s) 654, 693
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 36, 788, 893
AsuHPI GGTGA 1 cut(s) 764
AvaII GGWCC 3 cut(s) 36, 788, 893
BarI GAAGNNNNNNTAC 1 cut(s) 1033
BbsI GAAGAC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 220, 846
BccI CCATC 3 cut(s) 247, 575, 779
BceAI ACGGC 2 cut(s) 681, 957
BcoDI GTCTC 3 cut(s) 136, 864, 1051
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 884
BglII AGATCT 1 cut(s) 586
BisI GCNGC 3 cut(s) 234, 276, 835
BlsI GCNGC 3 cut(s) 235, 277, 836
Bme18I GGWCC 3 cut(s) 36, 788, 893
BmgT120I GGNCC 3 cut(s) 36, 788, 893
BmiI GGNNCC 1 cut(s) 37
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 4 cut(s) 376, 661, 862, 884
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 1005
BpmI CTGGAG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 2 cut(s) 482, 530
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 637, 993
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 797
Bse1I ACTGG 4 cut(s) 46, 102, 139, 771
Bse3DI GCAATG 1 cut(s) 775
Bse8I GATNNNNATC 1 cut(s) 585
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 637, 993
BseGI GGATG 1 cut(s) 925
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 58, 797
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 555, 1044
BseNI ACTGG 4 cut(s) 46, 102, 139, 771
BseXI GCAGC 2 cut(s) 220, 846
BsgI GTGCAG 1 cut(s) 219
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 738
BshVI ATCGAT 1 cut(s) 723
BsiEI CGRYCG 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 58, 797
BsmAI GTCTC 3 cut(s) 136, 864, 1051
BsnI GGCC 1 cut(s) 738
Bso31I GGTCTC 1 cut(s) 136
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 3 cut(s) 305, 586, 720
BspACI CCGC 2 cut(s) 33, 275
BspANI GGCC 1 cut(s) 738
BspCNI CTCAG 2 cut(s) 556, 1045
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 37
BspMAI CTGCAG 1 cut(s) 888
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 4 cut(s) 46, 102, 139, 771
BssECI CCNNGG 2 cut(s) 637, 993
BssMI GATC 3 cut(s) 305, 586, 720
BssT1I CCWWGG 2 cut(s) 637, 993
Bst4CI ACNGT 4 cut(s) 334, 683, 752, 761
Bst6I CTCTTC 3 cut(s) 134, 173, 1054
BstAPI GCANNNNNTGC 1 cut(s) 157
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 4 cut(s) 238, 300, 348, 839
BstDEI CTNAG 3 cut(s) 147, 564, 1053
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 925
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 3 cut(s) 308, 589, 723
BstMAI GTCTC 3 cut(s) 136, 864, 1051
BstMBI GATC 3 cut(s) 305, 586, 720
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 2 cut(s) 157, 347
BstSFI CTRYAG 1 cut(s) 884
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 220, 846
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 1 cut(s) 738
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 1 cut(s) 925
BtsIMutI CAGTG 1 cut(s) 688
Cac8I GCNNGC 4 cut(s) 238, 300, 348, 839
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 36, 788, 893
ClaI ATCGAT 1 cut(s) 723
Csp6I GTAC 3 cut(s) 7, 49, 990
CviAII CATG 3 cut(s) 316, 406, 578
CviQI GTAC 3 cut(s) 7, 49, 990
DdeI CTNAG 3 cut(s) 147, 564, 1053
DpnI GATC 3 cut(s) 307, 588, 722
DpnII GATC 3 cut(s) 305, 586, 720
Eam1104I CTCTTC 3 cut(s) 134, 173, 1054
EarI CTCTTC 3 cut(s) 134, 173, 1054
Eco130I CCWWGG 2 cut(s) 637, 993
Eco147I AGGCCT 1 cut(s) 738
Eco31I GGTCTC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 3 cut(s) 36, 788, 893
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 36
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 2 cut(s) 637, 993
EcoT22I ATGCAT 1 cut(s) 877
ErhI CCWWGG 2 cut(s) 637, 993
FaeI CATG 3 cut(s) 319, 409, 581
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FatI CATG 3 cut(s) 315, 405, 577
FauI CCCGC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 234, 276, 835
FokI GGATG 1 cut(s) 912
Fsp4HI GCNGC 3 cut(s) 234, 276, 835
FspBI CTAG 1 cut(s) 167
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 234, 276, 835
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 1 cut(s) 738
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 319, 409, 581
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 764
HindII GTYRAC 1 cut(s) 764
HindIII AAGCTT 2 cut(s) 172, 853
HinfI GANTC 4 cut(s) 602, 629, 805, 1044
HphI GGTGA 1 cut(s) 764
Hpy166II GTNNAC 2 cut(s) 7, 764
Hpy188I TCNGA 6 cut(s) 330, 529, 810, 826, 871, 1054
Hpy188III TCNNGA 6 cut(s) 442, 509, 578, 659, 791, 891
Hpy8I GTNNAC 2 cut(s) 7, 764
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 5 cut(s) 52, 177, 265, 495, 824
HpyCH4III ACNGT 4 cut(s) 334, 683, 752, 761
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 7 cut(s) 160, 236, 350, 572, 674, 875, 886
HpyF10VI GCNNNNNNNGC 2 cut(s) 157, 347
HpyF3I CTNAG 3 cut(s) 147, 564, 1053
HpySE526I ACGT 1 cut(s) 293
Hsp92II CATG 3 cut(s) 319, 409, 581
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 3 cut(s) 305, 586, 720
LmnI GCTCC 2 cut(s) 217, 230
Lsp1109I GCAGC 2 cut(s) 220, 846
LweI GCATC 4 cut(s) 376, 661, 862, 884
MaeI CTAG 1 cut(s) 167
MaeII ACGT 1 cut(s) 293
MalI GATC 3 cut(s) 307, 588, 722
MboI GATC 3 cut(s) 305, 586, 720
MboII GAAGA 8 cut(s) 151, 190, 304, 405, 794, 839, 1010, 1041
MflI RGATCY 1 cut(s) 586
MluCI AATT 5 cut(s) 208, 375, 447, 498, 539
MlyI GAGTC 2 cut(s) 638, 1053
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 9 cut(s) 60, 69, 94, 449, 457, 487, 543, 697, 706
Mph1103I ATGCAT 1 cut(s) 877
MroXI GAANNNNTTC 2 cut(s) 654, 693
MseI TTAA 1 cut(s) 900
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 157, 347
NdeII GATC 3 cut(s) 305, 586, 720
NlaIII CATG 3 cut(s) 319, 409, 581
NlaIV GGNNCC 1 cut(s) 37
NsiI ATGCAT 1 cut(s) 877
PagI TCATGA 1 cut(s) 577
PceI AGGCCT 1 cut(s) 738
PdmI GAANNNNTTC 2 cut(s) 654, 693
PfeI GAWTC 2 cut(s) 602, 805
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 3 cut(s) 235, 277, 836
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 2 cut(s) 637, 1052
PpsI GAGTC 2 cut(s) 637, 1052
PpuMI RGGWCCY 1 cut(s) 36
Psp5II RGGWCCY 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 3 cut(s) 36, 788, 893
PspPPI RGGWCCY 1 cut(s) 36
PstI CTGCAG 1 cut(s) 888
PsuI RGATCY 1 cut(s) 586
PvuI CGATCG 1 cut(s) 308
RsaI GTAC 3 cut(s) 8, 50, 991
RsaNI GTAC 3 cut(s) 7, 49, 990
SaqAI TTAA 1 cut(s) 900
SatI GCNGC 3 cut(s) 234, 276, 835
Sau3AI GATC 3 cut(s) 305, 586, 720
Sau96I GGNCC 3 cut(s) 36, 788, 893
SchI GAGTC 2 cut(s) 638, 1053
SfaNI GCATC 4 cut(s) 376, 661, 862, 884
SfcI CTRYAG 1 cut(s) 884
SinI GGWCC 3 cut(s) 36, 788, 893
SmlI CTYRAG 2 cut(s) 461, 509
SmoI CTYRAG 2 cut(s) 461, 509
Sse9I AATT 5 cut(s) 208, 375, 447, 498, 539
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 2 cut(s) 33, 275
SspMI CTAG 1 cut(s) 167
StuI AGGCCT 1 cut(s) 738
StyI CCWWGG 2 cut(s) 637, 993
TaaI ACNGT 4 cut(s) 334, 683, 752, 761
TaiI ACGT 1 cut(s) 296
TaqI TCGA 5 cut(s) 304, 358, 384, 702, 723
TasI AATT 5 cut(s) 208, 375, 447, 498, 539
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 602, 805
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TscAI CASTG 1 cut(s) 688
TseI GCWGC 2 cut(s) 233, 834
TspDTI ATGAA 3 cut(s) 71, 304, 663
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 1 cut(s) 688
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 3 cut(s) 36, 788, 893
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmnI GAANNNNTTC 2 cut(s) 654, 693
XspI CTAG 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.