Rh5AG258000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002487
Physical Location & Seq
Forward (+)
33623697 .. 33627637
3941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG258000.1

Sequence Viewer

Length: 1122 bp
ATGGATTCCGAAGCAGAGGCTCTTGAGGAGAAGTTGATGTCTTTGCTCGGTCAGCTCCGAGCTGAGCGTGCCATTCTTGAAAGAGTTGTCTACAAGAACAAGAATCAGCATAGACGTTCCTCTTACTTCCAATACCTAATGAAGGTAAGGAGGGATTTGAGGCTTTTACAATCAACTAAGTTGGAGGAGATTTTGGGCTGCTGTTTCCAAAGTATCACTGGAAAGAGACCCAAGCAAAAGATGCATCTATTAGAAAGTTTGAAGAGAAGAAAATGTGAAAGTGGAAAATATAATTTCATGGAACGACTTCTGGGAGCTGCACGTCTTCTATCGCAGATGGTTGAACCAATGTTGAAGGCTGCTATTCAGATATCTACATTGCTGGCTCGATCTTTTTTCATGGGATTCTCTTTGACTATCTTAGCTTTGCTTGCACGACTTCGAGTTTTGGTTCAACAGATTCTGCTTGATGTTGTTTCAGTATTCAACATGGTTTCTTCTCTTTCCCAAAAGAAGCAATCAGTTAAAATAAATCAAGAAGGAGTTGAGGTCTTCAGGGAGTTTTACCCTACACAAGAGGATTTTATCACTCTGGAGTGCGTGTGGAAATCTGATAAATTTGACTTGCTTGAGAGGACACATAAGAGCCAGATTGCAAGTCCTGGTGTGGATATTTCTTTAGGGACATCATCTGTGCTTTATCGAAGTATTGAGTCTTCCCTTGGAGATGATGAAATGTCTGAGAGGCCAGATGAAAACCATACTGTTGAGCTCGATCAAGTTCAGGTCATGGAGAACAACATTAATCTATTGTCTGGTCCATCCGATGGTGATGATGTAAAGCTGGTTGAACCTTGTGGGGAGGGTTCAAACATGGAAGACACTCCTAGCAAAAAGCTTTCCCAAGAAGGTTGCTTGCCACAAGTCTCTTCAAGTTCAGAGGCATCAAAACTGAAGTCTGGACCAGTTAAAGTGGCATTCGTACAAATTAAAAAACCCATAACATCATCCGCAAATACATCTGGACTCCATTCTAGAGAAACTGACATCATTAACCATAATAAGGAAGATCCATTGTTCAGCTTGCTTTCTAGTGCCAATGTAAAGAATAGTCTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4477

Protein Analysis

373

Amino Acids

42.0

Weight (kDa)

7.6

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEPRO_N PF14780 7 - 163 2.1e-14 Nucleolus and neural progenitor protein, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 827
AccI GTMKAC 1 cut(s) 90
AciI CCGC 1 cut(s) 1011
AclWI GGATC 1 cut(s) 1064
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 2 cut(s) 538, 974
AfaI GTAC 1 cut(s) 984
AfiI CCNNNNNNNGG 3 cut(s) 142, 827, 1063
AgsI TTSAA 9 cut(s) 80, 262, 344, 355, 455, 487, 851, 870, 933
AjiI CACGTC 1 cut(s) 323
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 8 cut(s) 55, 62, 317, 425, 772, 844, 898, 1083
AluI AGCT 8 cut(s) 55, 62, 317, 425, 772, 844, 898, 1083
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 3 cut(s) 220, 931, 1118
AlwI GGATC 1 cut(s) 1064
AlwNI CAGNNNCTG 1 cut(s) 463
AoxI GGCC 1 cut(s) 746
ApeKI GCWGC 3 cut(s) 198, 317, 359
ApoI RAATTY 1 cut(s) 617
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 818, 962
AsuHPI GGTGA 1 cut(s) 842
AvaII GGWCC 2 cut(s) 818, 962
BanII GRGCYC 1 cut(s) 774
BarI GAAGNNNNNNTAC 2 cut(s) 700, 732
BbsI GAAGAC 4 cut(s) 317, 544, 708, 885
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 185, 304, 346
BccI CCATC 3 cut(s) 331, 821, 829
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 220, 931, 1118
BfaI CTAG 3 cut(s) 888, 1035, 1092
BisI GCNGC 3 cut(s) 199, 318, 360
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 3 cut(s) 200, 319, 361
Bme1390I CCNGG 1 cut(s) 663
Bme18I GGWCC 2 cut(s) 818, 962
BmgBI CACGTC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 818, 962
BmrFI CCNGG 1 cut(s) 663
BmsI GCATC 3 cut(s) 231, 253, 953
BpiI GAAGAC 4 cut(s) 317, 544, 708, 885
BpmI CTGGAG 1 cut(s) 614
Bpu1102I GCTNAGC 1 cut(s) 63
BpuEI CTTGAG 2 cut(s) 44, 650
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 721
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 587, 617
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 827, 1063
Bse1I ACTGG 2 cut(s) 223, 965
Bse3DI GCAATG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 721
BseGI GGATG 2 cut(s) 821, 1007
BseLI CCNNNNNNNGG 3 cut(s) 142, 827, 1063
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 54, 732
BseNI ACTGG 2 cut(s) 223, 965
BseRI GAGGAG 2 cut(s) 41, 200
BseXI GCAGC 3 cut(s) 185, 304, 346
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 774
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 3 cut(s) 142, 827, 1063
BsmAI GTCTC 3 cut(s) 220, 931, 1118
BsmFI GGGAC 1 cut(s) 697
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 1 cut(s) 748
Bso31I GGTCTC 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 774
Bsp143I GATC 3 cut(s) 389, 775, 1069
Bsp1720I GCTNAGC 1 cut(s) 63
BspACI CCGC 1 cut(s) 1011
BspANI GGCC 1 cut(s) 748
BspCNI CTCAG 2 cut(s) 55, 733
BspPI GGATC 1 cut(s) 1064
BspTNI GGTCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 2 cut(s) 223, 965
BssECI CCNNGG 1 cut(s) 721
BssMI GATC 3 cut(s) 389, 775, 1069
BssT1I CCWWGG 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 1 cut(s) 766
Bst6I CTCTTC 2 cut(s) 257, 934
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 5 cut(s) 69, 384, 432, 917, 1085
BstDEI CTNAG 4 cut(s) 63, 177, 421, 741
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 2 cut(s) 821, 1007
BstKTI GATC 3 cut(s) 392, 778, 1072
BstMAI GTCTC 3 cut(s) 220, 931, 1118
BstMBI GATC 3 cut(s) 389, 775, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
BstNI CCWGG 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 661
BstV1I GCAGC 3 cut(s) 185, 304, 346
BstV2I GAAGAC 4 cut(s) 317, 544, 708, 885
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BsuRI GGCC 1 cut(s) 748
BtrI CACGTC 1 cut(s) 323
BtsCI GGATG 2 cut(s) 821, 1007
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 5 cut(s) 69, 384, 432, 917, 1085
CaiI CAGNNNCTG 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 818, 962
Csp6I GTAC 1 cut(s) 983
CviAII CATG 5 cut(s) 298, 400, 490, 790, 874
CviQI GTAC 1 cut(s) 983
DdeI CTNAG 4 cut(s) 63, 177, 421, 741
DpnI GATC 3 cut(s) 391, 777, 1071
DpnII GATC 3 cut(s) 389, 775, 1069
Eam1104I CTCTTC 2 cut(s) 257, 934
EarI CTCTTC 2 cut(s) 257, 934
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 1 cut(s) 721
Eco24I GRGCYC 1 cut(s) 774
Eco31I GGTCTC 1 cut(s) 220
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 818, 962
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 2 cut(s) 538, 974
EcoICRI GAGCTC 1 cut(s) 772
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 661
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 721
EcoT22I ATGCAT 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 1 cut(s) 721
FaeI CATG 5 cut(s) 301, 403, 493, 793, 877
FaqI GGGAC 1 cut(s) 697
FatI CATG 5 cut(s) 297, 399, 489, 789, 873
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 199, 318, 360
FokI GGATG 2 cut(s) 808, 994
FriOI GRGCYC 1 cut(s) 774
Fsp4HI GCNGC 3 cut(s) 199, 318, 360
FspBI CTAG 3 cut(s) 888, 1035, 1092
GluI GCNGC 3 cut(s) 199, 318, 360
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 1 cut(s) 748
Hin1II CATG 5 cut(s) 301, 403, 493, 793, 877
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 6 cut(s) 5, 103, 405, 460, 713, 1026
HphI GGTGA 1 cut(s) 842
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 8 cut(s) 10, 59, 369, 613, 742, 826, 940, 1121
Hpy188III TCNNGA 7 cut(s) 23, 77, 536, 593, 960, 1023, 1035
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 4 cut(s) 136, 349, 533, 902
HpyCH4III ACNGT 1 cut(s) 766
HpyCH4IV ACGT 2 cut(s) 115, 322
HpyCH4V TGCA 4 cut(s) 244, 320, 434, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
HpyF3I CTNAG 4 cut(s) 63, 177, 421, 741
HpySE526I ACGT 2 cut(s) 115, 322
Hsp92II CATG 5 cut(s) 301, 403, 493, 793, 877
Kzo9I GATC 3 cut(s) 389, 775, 1069
LmnI GCTCC 2 cut(s) 60, 314
Lsp1109I GCAGC 3 cut(s) 185, 304, 346
LweI GCATC 3 cut(s) 231, 253, 953
MaeI CTAG 3 cut(s) 888, 1035, 1092
MaeII ACGT 2 cut(s) 115, 322
MalI GATC 3 cut(s) 391, 777, 1071
MboI GATC 3 cut(s) 389, 775, 1069
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 1 cut(s) 774
MluCI AATT 3 cut(s) 292, 617, 987
MlyI GAGTC 2 cut(s) 722, 1020
MmeI TCCRAC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 5 cut(s) 525, 804, 969, 990, 1053
MspR9I CCNGG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
NdeII GATC 3 cut(s) 389, 775, 1069
NlaIII CATG 5 cut(s) 301, 403, 493, 793, 877
NsiI ATGCAT 1 cut(s) 246
PctI GAATGC 1 cut(s) 977
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 4 cut(s) 5, 103, 405, 460
PflMI CCANNNNNTGG 1 cut(s) 827
PkrI GCNGC 3 cut(s) 200, 319, 361
PleI GAGTC 2 cut(s) 721, 1020
PpsI GAGTC 2 cut(s) 721, 1020
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 774
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspPI GGNCC 2 cut(s) 818, 962
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 1 cut(s) 1069
RsaI GTAC 1 cut(s) 984
RsaNI GTAC 1 cut(s) 983
SacI GAGCTC 1 cut(s) 774
SaqAI TTAA 5 cut(s) 525, 804, 969, 990, 1053
SatI GCNGC 3 cut(s) 199, 318, 360
Sau3AI GATC 3 cut(s) 389, 775, 1069
Sau96I GGNCC 2 cut(s) 818, 962
SchI GAGTC 2 cut(s) 722, 1020
ScrFI CCNGG 1 cut(s) 663
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 3 cut(s) 231, 253, 953
SinI GGWCC 2 cut(s) 818, 962
SmlI CTYRAG 2 cut(s) 23, 629
SmoI CTYRAG 2 cut(s) 23, 629
Sse9I AATT 3 cut(s) 292, 617, 987
SsiI CCGC 1 cut(s) 1011
SspMI CTAG 3 cut(s) 888, 1035, 1092
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 1 cut(s) 766
TaiI ACGT 2 cut(s) 118, 325
TaqI TCGA 4 cut(s) 388, 442, 703, 774
TaqII GACCGA 1 cut(s) 38
TasI AATT 3 cut(s) 292, 617, 987
TfiI GAWTC 4 cut(s) 5, 103, 405, 460
Tru1I TTAA 5 cut(s) 525, 804, 969, 990, 1053
Tru9I TTAA 5 cut(s) 525, 804, 969, 990, 1053
TscAI CASTG 1 cut(s) 223
TseI GCWGC 3 cut(s) 198, 317, 359
TspDTI ATGAA 5 cut(s) 155, 286, 388, 747, 768
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 827
VpaK11BI GGWCC 2 cut(s) 818, 962
VspI ATTAAT 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 617
XbaI TCTAGA 1 cut(s) 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 888, 1035, 1092
Zsp2I ATGCAT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.