Rroxscaffold_4G00319700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002487
Physical Location & Seq
Forward (+)
47802286 .. 47807530
5245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319700.1

Sequence Viewer

Length: 744 bp
ATGGATTCTGAAGCAGAAGCTCTTGAGGAGAAGCTGACGTGTTTGTTTGGTCAGCTCCGAGCTGAGTGTGCTATTCTTGAAACAGTGGTCTACAAGAACAAGAACCAGCATAGACGTTCCTCGTACTTCCAGTACCTAATGAAGGTAAGGAGGGATTTGAGGCTTTTACAATCAATTAAGTTGGAGGAGATTTTGGGCTGCTGTTTCCAAAGTATCACTGGAAAGAGACCCAAGCAAAAGGTGCATCTATTAGAAAGTTTGAAGAGAAGAAAATGTGAAAGTGGAAAATATAATTTCATGGAACGACTTCTGGGAGCAGCACGTCTTCTATCACAGATGGTTGAACCAATGTTGAAGGCTGCTATTCAGATATCTGCATTGCTGGCTCGATCTTTTTTCATGGGATTCTCTTTGACTGTCTTAGCTTTGCTTGCACGACTTCGAGTTTTGGTTCAACAGATTCTTGTTGATGTTGTTTCAGTATTCAACATGGTTTCTTCTCTCTCCCAAAAGAAGCAATCAGTTAAAATAAATCAAGGAGTTGAGGTCTTCAGGGAGTTTTACCCTACACAAGAGGATTTTATCACTCTGGAGTGCGTGTGGAAATCTGATAAATTTGACTTGCTTGAGAGGACACAAAAGAGCCAGATTGCAAGTCCTGGTGTTGATCTTTCTTTAGGGACATCATCTGTGCTTTATCGAAGTATTGAGTCTTCCCTTGGAGGTCCCACGGGTGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4477

Protein Analysis

247

Amino Acids

28.13

Weight (kDa)

9.39

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEPRO_N PF14780 18 - 163 2.1e-14 Nucleolus and neural progenitor protein, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 2 cut(s) 30, 535
AfaI GTAC 2 cut(s) 125, 134
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 6 cut(s) 80, 262, 344, 355, 455, 487
AjiI CACGTC 2 cut(s) 39, 323
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 5 cut(s) 20, 34, 55, 62, 425
AluI AGCT 5 cut(s) 20, 34, 55, 62, 425
Alw26I GTCTC 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 198, 317, 359
ApoI RAATTY 1 cut(s) 614
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 725
AvaII GGWCC 1 cut(s) 725
BarI GAAGNNNNNNTAC 2 cut(s) 697, 729
BbsI GAAGAC 3 cut(s) 317, 541, 705
BbvI GCAGC 3 cut(s) 185, 329, 346
BccI CCATC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 660
BcoDI GTCTC 1 cut(s) 220
BisI GCNGC 3 cut(s) 199, 318, 360
BlsI GCNGC 3 cut(s) 200, 319, 361
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 1 cut(s) 725
BmgBI CACGTC 2 cut(s) 39, 323
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 1 cut(s) 727
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 253
BpiI GAAGAC 3 cut(s) 317, 541, 705
BpmI CTGGAG 1 cut(s) 611
BpuEI CTTGAG 2 cut(s) 44, 647
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 718, 729
BsaXI ACNNNNNCTCC 4 cut(s) 531, 561, 584, 614
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 130, 223
Bse3DI GCAATG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 2 cut(s) 718, 729
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 130, 223
BseRI GAGGAG 2 cut(s) 41, 200
BseXI GCAGC 3 cut(s) 185, 329, 346
BslFI GGGAC 2 cut(s) 694, 711
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 2 cut(s) 694, 711
Bso31I GGTCTC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 389, 667
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 727
BspTNI GGTCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 2 cut(s) 130, 223
BssECI CCNNGG 2 cut(s) 718, 729
BssMI GATC 2 cut(s) 389, 667
BssT1I CCWWGG 1 cut(s) 718
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 85, 418
Bst6I CTCTTC 1 cut(s) 257
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 384, 432
BstDEI CTNAG 2 cut(s) 63, 421
BstDSI CCRYGG 1 cut(s) 729
BstENI CCTNNNNNAGG 1 cut(s) 140
BstKTI GATC 2 cut(s) 392, 670
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 389, 667
BstMWI GCNNNNNNNGC 4 cut(s) 68, 241, 383, 431
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 658
BstV1I GCAGC 3 cut(s) 185, 329, 346
BstV2I GAAGAC 3 cut(s) 317, 541, 705
BtgI CCRYGG 1 cut(s) 729
BtrI CACGTC 2 cut(s) 39, 323
BtsIMutI CAGTG 2 cut(s) 90, 216
Cac8I GCNNGC 2 cut(s) 384, 432
Cfr13I GGNCC 1 cut(s) 725
Csp6I GTAC 2 cut(s) 124, 133
CviAII CATG 3 cut(s) 298, 400, 490
CviQI GTAC 2 cut(s) 124, 133
DdeI CTNAG 2 cut(s) 63, 421
DpnI GATC 2 cut(s) 391, 669
DpnII GATC 2 cut(s) 389, 667
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 718
Eco31I GGTCTC 1 cut(s) 220
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 725
Eco57I CTGAAG 2 cut(s) 30, 535
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 725
EcoRII CCWGG 1 cut(s) 658
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 3 cut(s) 301, 403, 493
FaiI YATR 5 cut(s) 111, 291, 299, 401, 491
FaqI GGGAC 2 cut(s) 694, 711
FatI CATG 3 cut(s) 297, 399, 489
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 199, 318, 360
Fsp4HI GCNGC 3 cut(s) 199, 318, 360
GluI GCNGC 3 cut(s) 199, 318, 360
GsuI CTGGAG 1 cut(s) 611
Hin1II CATG 3 cut(s) 301, 403, 493
HinfI GANTC 4 cut(s) 5, 405, 460, 710
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 4 cut(s) 10, 59, 369, 610
Hpy188III TCNNGA 3 cut(s) 23, 77, 590
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 136, 349
HpyCH4III ACNGT 2 cut(s) 85, 418
HpyCH4IV ACGT 3 cut(s) 38, 115, 322
HpyCH4V TGCA 4 cut(s) 244, 377, 434, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 241, 383, 431
HpyF3I CTNAG 2 cut(s) 63, 421
HpySE526I ACGT 3 cut(s) 38, 115, 322
Hsp92II CATG 3 cut(s) 301, 403, 493
Kzo9I GATC 2 cut(s) 389, 667
LmnI GCTCC 2 cut(s) 60, 314
Lsp1109I GCAGC 3 cut(s) 185, 329, 346
LweI GCATC 1 cut(s) 253
MaeII ACGT 3 cut(s) 38, 115, 322
MalI GATC 2 cut(s) 391, 669
MboI GATC 2 cut(s) 389, 667
MboII GAAGA 6 cut(s) 274, 279, 317, 489, 541, 705
MluCI AATT 3 cut(s) 174, 292, 614
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 9 cut(s) 19, 130, 144, 153, 178, 538, 568, 624, 716
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 2 cut(s) 177, 525
MspR9I CCNGG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 4 cut(s) 68, 241, 383, 431
NdeII GATC 2 cut(s) 389, 667
NlaIII CATG 3 cut(s) 301, 403, 493
NlaIV GGNNCC 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 3 cut(s) 5, 405, 460
PkrI GCNGC 3 cut(s) 200, 319, 361
PleI GAGTC 1 cut(s) 718
PpsI GAGTC 1 cut(s) 718
PpuMI RGGWCCY 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 1 cut(s) 727
PspPI GGNCC 1 cut(s) 725
PspPPI RGGWCCY 1 cut(s) 725
RsaI GTAC 2 cut(s) 125, 134
RsaNI GTAC 2 cut(s) 124, 133
SaqAI TTAA 2 cut(s) 177, 525
SatI GCNGC 3 cut(s) 199, 318, 360
Sau3AI GATC 2 cut(s) 389, 667
Sau96I GGNCC 1 cut(s) 725
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 660
SfaNI GCATC 1 cut(s) 253
SinI GGWCC 1 cut(s) 725
SmlI CTYRAG 2 cut(s) 23, 626
SmoI CTYRAG 2 cut(s) 23, 626
Sse9I AATT 3 cut(s) 174, 292, 614
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 2 cut(s) 85, 418
TaiI ACGT 3 cut(s) 41, 118, 325
TaqI TCGA 3 cut(s) 388, 442, 700
TasI AATT 3 cut(s) 174, 292, 614
TfiI GAWTC 3 cut(s) 5, 405, 460
Tru1I TTAA 2 cut(s) 177, 525
Tru9I TTAA 2 cut(s) 177, 525
TscAI CASTG 2 cut(s) 90, 223
TseI GCWGC 3 cut(s) 198, 317, 359
TspDTI ATGAA 3 cut(s) 155, 286, 388
TspRI CASTG 2 cut(s) 90, 223
VpaK11BI GGWCC 1 cut(s) 725
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.