Rh5DG270200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002487
Physical Location & Seq
Forward (+)
32907061 .. 32911797
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG270200.1

Sequence Viewer

Length: 861 bp
ATGGATTCCGAAGCAGAGGCTCTTGAGGAGAAGTTGATGTATTTGCTCGGTCAGCTCCGAGCTGAGTGTGCCATTCTTGAAAGAGTGGTCTACAAGAACAAGAATCAGCATAGACGTTCCTCTTACTTCCAGTACCTAATGAAGGTAAGGAGGGATTTGAGGCTTTTACAATCAACTAAGTTGGAGGAGATTTTGGGCTGCTGTTTCCAAACTATCACTGGAAAGAGACCAAAGCAAAAGATGCATCTATTAGAAAGTTTGAAGAGAAGAAAATGTGGAAGTGGAAAATATAATTTCATGGAACGACTTCTGGGAGCTGCACGTCTTCTATCGCAGATGGTTGAACCAATGTTGAAGGCTGCTATTCAGATATCTACATTGCTGGCTCGATCTTTTTTCATGGGATTCTCTTTGACTATCTTAGCTTTGCTTGCACGACTTCGAGTTTTGGTTCAACAGATTCTGCTTGATGTTGTTTCAGTATTCAACATGGTTTCTTCTCTTTCCCAAAAGAAGCAATCAGTTAAAATAAATCAAGAAGGAGTTGAGGTCTTCAGGGAGTTTTACCCTACACAAGAGGATTTTATCACTCTGGAGTGCGTGTGGAAATCTGATAAATTTGACTTGCTTGAGAGGACACATAAAAGCCAGATTGCAAGTCCTGGTGTGGATATTTCTTTAGGGACATCATCTGTGCTTTATCGAAGTATTGAGTCTTCCCTTGGAGGTCAGTGTGTTTACTGTCATTCTTATTTTATCTTTCTATATATAAGCATATCATCCTTCAATATTATTCTTGTTTGCACGTCTCAATTTTTTTTTCATTTCCACCATTTTCACTGTTTAATATCTGTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4477

Protein Analysis

286

Amino Acids

33.05

Weight (kDa)

9.25

Isoelectric Point (pI)

59.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEPRO_N PF14780 7 - 163 1.7e-13 Nucleolus and neural progenitor protein, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 538
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 7 cut(s) 80, 262, 344, 355, 455, 487, 787
AjiI CACGTC 2 cut(s) 323, 807
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 4 cut(s) 55, 62, 317, 425
AluI AGCT 4 cut(s) 55, 62, 317, 425
Alw26I GTCTC 2 cut(s) 220, 813
AlwNI CAGNNNCTG 1 cut(s) 463
ApeKI GCWGC 3 cut(s) 198, 317, 359
ApoI RAATTY 1 cut(s) 617
Asp700I GAANNNNTTC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 700, 732
BbsI GAAGAC 3 cut(s) 317, 544, 708
BbvI GCAGC 3 cut(s) 185, 304, 346
BccI CCATC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 220, 813
BisI GCNGC 3 cut(s) 199, 318, 360
BlsI GCNGC 3 cut(s) 200, 319, 361
Bme1390I CCNGG 1 cut(s) 663
BmgBI CACGTC 2 cut(s) 323, 807
BmrFI CCNGG 1 cut(s) 663
BmsI GCATC 2 cut(s) 231, 253
BpiI GAAGAC 3 cut(s) 317, 544, 708
BpmI CTGGAG 1 cut(s) 614
BpuEI CTTGAG 2 cut(s) 44, 650
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 721
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 587, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 130, 223
Bse3DI GCAATG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 721
BseGI GGATG 1 cut(s) 779
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 130, 223
BseRI GAGGAG 2 cut(s) 41, 200
BseXI GCAGC 3 cut(s) 185, 304, 346
BsgI GTGCAG 1 cut(s) 303
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 220, 813
BsmBI CGTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 697
Bso31I GGTCTC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 389
BspCNI CTCAG 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 377
BsrI ACTGG 2 cut(s) 130, 223
BssECI CCNNGG 1 cut(s) 721
BssMI GATC 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 2 cut(s) 743, 842
Bst6I CTCTTC 1 cut(s) 257
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 384, 432
BstDEI CTNAG 3 cut(s) 63, 177, 421
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 779
BstKTI GATC 1 cut(s) 392
BstMAI GTCTC 2 cut(s) 220, 813
BstMBI GATC 1 cut(s) 389
BstMWI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
BstNI CCWGG 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 661
BstV1I GCAGC 3 cut(s) 185, 304, 346
BstV2I GAAGAC 3 cut(s) 317, 544, 708
BtrI CACGTC 2 cut(s) 323, 807
BtsCI GGATG 1 cut(s) 779
BtsIMutI CAGTG 3 cut(s) 216, 737, 838
Cac8I GCNNGC 2 cut(s) 384, 432
CaiI CAGNNNCTG 1 cut(s) 463
Csp6I GTAC 1 cut(s) 133
CviAII CATG 4 cut(s) 298, 400, 490, 858
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 3 cut(s) 63, 177, 421
DpnI GATC 1 cut(s) 391
DpnII GATC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 220
Eco32I GATATC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 538
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 661
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 721
EcoT22I ATGCAT 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 721
Esp3I CGTCTC 1 cut(s) 813
FaeI CATG 4 cut(s) 301, 403, 493, 861
FaqI GGGAC 1 cut(s) 697
FatI CATG 4 cut(s) 297, 399, 489, 857
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 199, 318, 360
FokI GGATG 1 cut(s) 766
Fsp4HI GCNGC 3 cut(s) 199, 318, 360
GluI GCNGC 3 cut(s) 199, 318, 360
GsuI CTGGAG 1 cut(s) 614
Hin1II CATG 4 cut(s) 301, 403, 493, 861
HinfI GANTC 5 cut(s) 5, 103, 405, 460, 713
Hpy166II GTNNAC 2 cut(s) 91, 739
Hpy188I TCNGA 4 cut(s) 10, 59, 369, 613
Hpy188III TCNNGA 4 cut(s) 23, 77, 536, 593
Hpy8I GTNNAC 2 cut(s) 91, 739
HpyAV CCTTC 4 cut(s) 136, 349, 533, 793
HpyCH4III ACNGT 2 cut(s) 743, 842
HpyCH4IV ACGT 3 cut(s) 115, 322, 806
HpyCH4V TGCA 5 cut(s) 244, 320, 434, 656, 804
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
HpyF3I CTNAG 3 cut(s) 63, 177, 421
HpySE526I ACGT 3 cut(s) 115, 322, 806
Hsp92II CATG 4 cut(s) 301, 403, 493, 861
Kzo9I GATC 1 cut(s) 389
LmnI GCTCC 2 cut(s) 60, 314
LpnPI CCDG 9 cut(s) 143, 204, 296, 368, 541, 578, 648, 662, 675
Lsp1109I GCAGC 3 cut(s) 185, 304, 346
LweI GCATC 2 cut(s) 231, 253
MaeII ACGT 3 cut(s) 115, 322, 806
MaeIII GTNAC 1 cut(s) 853
MalI GATC 1 cut(s) 391
MboI GATC 1 cut(s) 389
MboII GAAGA 6 cut(s) 274, 279, 317, 489, 544, 708
MluCI AATT 3 cut(s) 292, 617, 812
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 2 cut(s) 525, 845
MspR9I CCNGG 1 cut(s) 663
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 4 cut(s) 52, 68, 241, 431
NdeII GATC 1 cut(s) 389
NlaIII CATG 4 cut(s) 301, 403, 493, 861
NmuCI GTSAC 1 cut(s) 853
NsiI ATGCAT 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 4 cut(s) 5, 103, 405, 460
PkrI GCNGC 3 cut(s) 200, 319, 361
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PstNI CAGNNNCTG 1 cut(s) 463
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SaqAI TTAA 2 cut(s) 525, 845
SatI GCNGC 3 cut(s) 199, 318, 360
Sau3AI GATC 1 cut(s) 389
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 663
SfaNI GCATC 2 cut(s) 231, 253
SmlI CTYRAG 2 cut(s) 23, 629
SmoI CTYRAG 2 cut(s) 23, 629
Sse9I AATT 3 cut(s) 292, 617, 812
SspI AATATT 1 cut(s) 790
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 2 cut(s) 743, 842
TaiI ACGT 3 cut(s) 118, 325, 809
TaqI TCGA 3 cut(s) 388, 442, 703
TaqII GACCGA 1 cut(s) 38
TasI AATT 3 cut(s) 292, 617, 812
TfiI GAWTC 4 cut(s) 5, 103, 405, 460
Tru1I TTAA 2 cut(s) 525, 845
Tru9I TTAA 2 cut(s) 525, 845
TscAI CASTG 3 cut(s) 223, 737, 845
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 3 cut(s) 198, 317, 359
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 4 cut(s) 155, 286, 388, 812
TspRI CASTG 3 cut(s) 223, 737, 845
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 306
Zsp2I ATGCAT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.