MD12G1050000.v1.1

Defensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003779
Physical Location & Seq
Reverse (-)
5669529 .. 5670042
514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1050000.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCTACTCTATCTGGAGCTTTTTCCACCGTCCTTGTCTTGCTTCTTATATTCGCTGTAGGGACACTCATGCCCATGGTGAAAGGTGACGTTTGCACCGAAGATATGGGTGCATGCGATCCTGACTGCAGTTCAAGGTGCACATCTGCACATCCTGGGGCTAAGGCCGGTTGTGATTTGAACAACAACAACCCGGTTTGCAGATGCTTCTACGACTGTGGACCTCCATCGCCGCCGCCATACTCGAAAAAGTGCAAAATCAGCTTGGGAATATGGGGTGAGAACTGCTCAGAGAGTGATTGTAACTCACAGTGTGCTTCCAAATATCCTGGACCTCAAGAAGGAAGTGGATACTGTTATAGCGTTGGAGCTCCTTTCTATACTTCTTGTTTTTGTGAGTACAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLR1-BP

Protein Analysis

137

Amino Acids

14.39

Weight (kDa)

4.93

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 236
AclWI GGATC 1 cut(s) 113
AdeI CACNNNGTG 1 cut(s) 314
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 2 cut(s) 135, 181
AjnI CCWGG 2 cut(s) 154, 328
AluBI AGCT 3 cut(s) 20, 264, 371
AluI AGCT 3 cut(s) 20, 264, 371
Alw21I GWGCWC 2 cut(s) 143, 373
Alw44I GTGCAC 1 cut(s) 139
AlwI GGATC 1 cut(s) 113
AoxI GGCC 1 cut(s) 165
ApaLI GTGCAC 1 cut(s) 139
AspS9I GGNCC 2 cut(s) 221, 332
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 91, 98, 290
AvaII GGWCC 2 cut(s) 221, 332
BaeGI GKGCMC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 373
Bbv12I GWGCWC 2 cut(s) 143, 373
BccI CCATC 1 cut(s) 235
BciT130I CCWGG 2 cut(s) 156, 330
BciVI GTATCC 1 cut(s) 344
BcnI CCSGG 1 cut(s) 194
BfmI CTRYAG 2 cut(s) 57, 127
BfuI GTATCC 1 cut(s) 344
BisI GCNGC 2 cut(s) 233, 236
BlsI GCNGC 2 cut(s) 234, 237
Bme1390I CCNGG 3 cut(s) 156, 194, 330
Bme18I GGWCC 2 cut(s) 221, 332
BmgT120I GGNCC 2 cut(s) 221, 332
BmrFI CCNGG 3 cut(s) 156, 194, 330
BmsI GCATC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 75, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse118I RCCGGY 1 cut(s) 167
BseBI CCWGG 2 cut(s) 156, 330
BseDI CCNNGG 2 cut(s) 75, 155
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMII CTCAG 1 cut(s) 303
BseSI GKGCMC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 167
BsiHKAI GWGCWC 2 cut(s) 143, 373
BsiSI CCGG 2 cut(s) 168, 194
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 143, 373
Bsp143I GATC 1 cut(s) 118
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 2 cut(s) 233, 236
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 302
BspMAI CTGCAG 1 cut(s) 131
BspPI GGATC 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 75, 155
BssMI GATC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 156, 330
Bst4CI ACNGT 4 cut(s) 31, 218, 312, 356
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 162, 289
BstDSI CCRYGG 1 cut(s) 75
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 156, 330
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 3 cut(s) 154, 192, 328
BstSFI CTRYAG 2 cut(s) 57, 127
BstSLI GKGCMC 1 cut(s) 143
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 221, 332
Csp6I GTAC 1 cut(s) 400
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 3 cut(s) 70, 76, 114
CviJI RGCY 6 cut(s) 5, 20, 161, 167, 264, 371
CviKI_1 RGCY 6 cut(s) 5, 20, 161, 167, 264, 371
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 162, 289
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
DraIII CACNNNGTG 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 221, 332
Eco53kI GAGCTC 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 154, 328
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 3 cut(s) 73, 79, 117
FaiI YATR 9 cut(s) 50, 71, 77, 107, 115, 241, 274, 360, 381
FaqI GGGAC 1 cut(s) 76
FatI CATG 3 cut(s) 69, 75, 113
Fnu4HI GCNGC 2 cut(s) 233, 236
FokI GGATG 1 cut(s) 138
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 2 cut(s) 233, 236
GluI GCNGC 2 cut(s) 233, 236
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 1 cut(s) 167
HapII CCGG 2 cut(s) 168, 194
Hin1II CATG 3 cut(s) 73, 79, 117
HpaII CCGG 2 cut(s) 168, 194
HphI GGTGA 3 cut(s) 91, 98, 290
Hpy166II GTNNAC 2 cut(s) 141, 221
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 3 cut(s) 15, 122, 338
Hpy8I GTNNAC 2 cut(s) 141, 221
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 4 cut(s) 31, 218, 312, 356
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 7 cut(s) 96, 113, 129, 141, 149, 201, 255
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 162, 289
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 3 cut(s) 73, 79, 117
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 3 cut(s) 17, 368, 376
LpnPI CCDG 7 cut(s) 135, 141, 168, 181, 207, 315, 342
LweI GCATC 1 cut(s) 194
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 86, 302
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 1 cut(s) 113
MhlI GDGCHC 2 cut(s) 143, 373
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 2 cut(s) 234, 345
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 2 cut(s) 168, 194
MspR9I CCNGG 3 cut(s) 156, 194, 330
MvaI CCWGG 2 cut(s) 156, 330
MwoI GCNNNNNNNGC 1 cut(s) 261
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 75
NdeII GATC 1 cut(s) 118
NlaIII CATG 3 cut(s) 73, 79, 117
NmuCI GTSAC 1 cut(s) 86
NspI RCATGY 1 cut(s) 117
PaeI GCATGC 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 2 cut(s) 234, 237
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 154, 328
PspGI CCWGG 2 cut(s) 154, 328
PspPI GGNCC 2 cut(s) 221, 332
PstI CTGCAG 1 cut(s) 131
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 373
SatI GCNGC 2 cut(s) 233, 236
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 2 cut(s) 221, 332
ScrFI CCNGG 3 cut(s) 156, 194, 330
SduI GDGCHC 2 cut(s) 143, 373
SetI ASST 8 cut(s) 22, 88, 93, 140, 226, 266, 337, 373
SfaNI GCATC 1 cut(s) 194
SfcI CTRYAG 2 cut(s) 57, 127
SinI GGWCC 2 cut(s) 221, 332
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SphI GCATGC 1 cut(s) 117
SsiI CCGC 2 cut(s) 233, 236
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 3 cut(s) 154, 192, 328
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 4 cut(s) 31, 218, 312, 356
TaiI ACGT 1 cut(s) 93
TaqI TCGA 1 cut(s) 245
TatI WGTACW 1 cut(s) 399
TauI GCSGC 2 cut(s) 235, 238
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 86
TspRI CASTG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 221, 332
XagI CCTNNNNNAGG 1 cut(s) 339
XceI RCATGY 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.