Rroxscaffold_6G00398330

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003779
Physical Location & Seq
Forward (+)
20220364 .. 20224786
4423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398330.1

Sequence Viewer

Length: 732 bp
ATGAAGAACTTGGCGAAGTTTTGCTTTGTTGACGACTTGAAGATGATAGATGATCAAGGGCTGCAGAGGCTTGATCTTGATCGGACCGAACCACAAGAAGTGTCACGTGGCGAGTATGGCTTTGAGGCGGATGAATCTGCACATTTGCTTGGTGCATCACTATCGATCTATTCGGGTGTTTTGGTGGAAGACCTACGTGCTCTTTTACAACAGTTGATTATTGCCAAGCTTGTTCATACAAGGAGGTATTTGGATCAAAGAAGAAGAAAAGAAGCAAAAGAGGGATTGAAAGGCAAATCAGCAAATCTGCTGTGCAGAACAGTCAACTTCGACAGAGCACTGAGACTAGCTCAGGATGAACCAGATGATGATATTTATATATATGGAAAGCCTAGGATATCTAGACCGAGCTTAGTTTCATCCTTGTCCGAAACCCCACTTCTTATAACTGCAAAGTTGAGCCATTCGGACTTGGTGAAAAAAACACTCGAACTGCAGCCCGAGTTCGCCACCGAGTTGGACTCGCGCAGGTCGACCCCGCTCCACCCCGACGGCGGCCAAGGGACACGTGGGGGTCGTGAGGGAGCTGCTCGGGCTGACGTGGCTGCGTGTTTGGTGAGGAACCGAATGGGAGGACGGTGCTCCACGCAGCGGTTGTTAAAAGAAGAGTCGAGGTGGTGGTGGGGGGAGTTGATCCGAGCCGACCGAGTTGATTCGGGCGTTGACGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

243

Amino Acids

27.33

Weight (kDa)

7.68

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 519
AccBSI CCGCTC 1 cut(s) 541
AccI GTMKAC 1 cut(s) 533
AccII CGCG 1 cut(s) 526
AciI CCGC 4 cut(s) 128, 539, 555, 652
AclWI GGATC 2 cut(s) 261, 688
AcoI YGGCCR 1 cut(s) 556
AcvI CACGTG 2 cut(s) 107, 569
AfiI CCNNNNNNNGG 2 cut(s) 554, 651
AflIII ACRYGT 1 cut(s) 566
AgsI TTSAA 2 cut(s) 40, 289
AjiI CACGTC 1 cut(s) 601
AluBI AGCT 4 cut(s) 229, 350, 411, 587
AluI AGCT 4 cut(s) 229, 350, 411, 587
Alw21I GWGCWC 3 cut(s) 202, 340, 644
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 2 cut(s) 261, 688
Ama87I CYCGRG 2 cut(s) 500, 591
AoxI GGCC 1 cut(s) 556
ApeKI GCWGC 5 cut(s) 61, 496, 587, 605, 649
AspA2I CCTAGG 1 cut(s) 392
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 487, 628
AvaI CYCGRG 2 cut(s) 500, 591
AvaII GGWCC 1 cut(s) 84
AvrII CCTAGG 1 cut(s) 392
BbrPI CACGTG 2 cut(s) 107, 569
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 3 cut(s) 202, 340, 644
BbvI GCAGC 5 cut(s) 48, 508, 574, 592, 661
BceAI ACGGC 1 cut(s) 568
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 3 cut(s) 347, 393, 402
BfmI CTRYAG 2 cut(s) 62, 494
BfuAI ACCTGC 1 cut(s) 519
BisI GCNGC 6 cut(s) 62, 497, 556, 588, 606, 650
BlnI CCTAGG 1 cut(s) 392
BlsI GCNGC 6 cut(s) 63, 498, 557, 589, 607, 651
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 2 cut(s) 500, 591
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 623
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 4 cut(s) 334, 366, 506, 538
Bpu10I CCTNAGC 1 cut(s) 351
Bsa29I ATCGAT 1 cut(s) 164
BsaAI YACGTR 3 cut(s) 107, 197, 569
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 392, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 651
Bse8I GATNNNNATC 1 cut(s) 78
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 392, 559
BseGI GGATG 3 cut(s) 136, 361, 419
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 554, 651
BseMII CTCAG 2 cut(s) 332, 365
BseXI GCAGC 5 cut(s) 48, 508, 574, 592, 661
BsgI GTGCAG 2 cut(s) 123, 334
Bsh1236I CGCG 1 cut(s) 526
Bsh1285I CGRYCG 1 cut(s) 706
BshFI GGCC 1 cut(s) 558
BshVI ATCGAT 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 706
BsiHKAI GWGCWC 3 cut(s) 202, 340, 644
BsiHKCI CYCGRG 2 cut(s) 500, 591
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 2 cut(s) 554, 651
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 577
BsnI GGCC 1 cut(s) 558
BsoBI CYCGRG 2 cut(s) 500, 591
Bsp1286I GDGCHC 3 cut(s) 202, 340, 644
Bsp143I GATC 6 cut(s) 52, 73, 79, 165, 253, 693
BspACI CCGC 4 cut(s) 128, 539, 555, 652
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 2 cut(s) 333, 364
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 2 cut(s) 66, 498
BspMI ACCTGC 1 cut(s) 519
BspPI GGATC 2 cut(s) 261, 688
BsrBI CCGCTC 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 392, 559
BssMI GATC 6 cut(s) 52, 73, 79, 165, 253, 693
BssT1I CCWWGG 2 cut(s) 392, 559
Bst4CI ACNGT 3 cut(s) 213, 322, 639
Bst6I CTCTTC 1 cut(s) 660
BstBAI YACGTR 3 cut(s) 107, 197, 569
BstDEI CTNAG 3 cut(s) 341, 351, 412
BstF5I GGATG 3 cut(s) 136, 361, 419
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 6 cut(s) 55, 76, 82, 168, 256, 696
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 6 cut(s) 52, 73, 79, 165, 253, 693
BstMCI CGRYCG 1 cut(s) 706
BstMWI GCNNNNNNNGC 4 cut(s) 67, 117, 593, 602
BstSFI CTRYAG 2 cut(s) 62, 494
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 5 cut(s) 48, 508, 574, 592, 661
BstV2I GAAGAC 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 164
BsuRI GGCC 1 cut(s) 558
BsuTUI ATCGAT 1 cut(s) 164
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 3 cut(s) 136, 361, 419
BtsIMutI CAGTG 1 cut(s) 338
BveI ACCTGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 528
Cfr13I GGNCC 1 cut(s) 84
ClaI ATCGAT 1 cut(s) 164
CpoI CGGWCCG 1 cut(s) 84
CspI CGGWCCG 1 cut(s) 84
DdeI CTNAG 3 cut(s) 341, 351, 412
DpnI GATC 6 cut(s) 54, 75, 81, 167, 255, 695
DpnII GATC 6 cut(s) 52, 73, 79, 165, 253, 693
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 392, 559
Eco32I GATATC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 84
Eco72I CACGTG 2 cut(s) 107, 569
Eco88I CYCGRG 2 cut(s) 500, 591
EcoRV GATATC 1 cut(s) 399
EcoT14I CCWWGG 2 cut(s) 392, 559
ErhI CCWWGG 2 cut(s) 392, 559
FaiI YATR 7 cut(s) 117, 237, 378, 380, 382, 384, 446
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 577
FauI CCCGC 1 cut(s) 546
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 533
Fnu4HI GCNGC 6 cut(s) 62, 497, 556, 588, 606, 650
FokI GGATG 3 cut(s) 143, 368, 406
Fsp4HI GCNGC 6 cut(s) 62, 497, 556, 588, 606, 650
FspBI CTAG 3 cut(s) 347, 393, 402
GlaI GCGC 1 cut(s) 527
GluI GCNGC 6 cut(s) 62, 497, 556, 588, 606, 650
HaeIII GGCC 1 cut(s) 558
HhaI GCGC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 4 cut(s) 31, 325, 534, 724
HindII GTYRAC 4 cut(s) 31, 325, 534, 724
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 134, 521, 668, 713
HphI GGTGA 2 cut(s) 487, 628
Hpy166II GTNNAC 4 cut(s) 31, 325, 534, 724
Hpy188I TCNGA 4 cut(s) 84, 430, 469, 698
Hpy188III TCNNGA 4 cut(s) 77, 353, 402, 578
Hpy8I GTNNAC 4 cut(s) 31, 325, 534, 724
Hpy99I CGWCG 1 cut(s) 554
HpyCH4III ACNGT 3 cut(s) 213, 322, 639
HpyCH4IV ACGT 4 cut(s) 106, 196, 568, 600
HpyCH4V TGCA 6 cut(s) 64, 140, 155, 315, 452, 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 117, 593, 602
HpyF3I CTNAG 3 cut(s) 341, 351, 412
HpySE526I ACGT 4 cut(s) 106, 196, 568, 600
HspAI GCGC 1 cut(s) 526
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 6 cut(s) 52, 73, 79, 165, 253, 693
LmnI GCTCC 3 cut(s) 546, 584, 647
LpnPI CCDG 3 cut(s) 338, 375, 514
Lsp1109I GCAGC 5 cut(s) 48, 508, 574, 592, 661
LweI GCATC 1 cut(s) 164
MaeI CTAG 3 cut(s) 347, 393, 402
MaeII ACGT 4 cut(s) 106, 196, 568, 600
MaeIII GTNAC 1 cut(s) 102
MalI GATC 6 cut(s) 54, 75, 81, 167, 255, 695
MbiI CCGCTC 1 cut(s) 541
MboI GATC 6 cut(s) 52, 73, 79, 165, 253, 693
MboII GAAGA 6 cut(s) 16, 52, 200, 273, 276, 677
MhlI GDGCHC 3 cut(s) 202, 340, 644
MlyI GAGTC 2 cut(s) 515, 677
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 8 cut(s) 60, 118, 237, 274, 574, 612, 626, 666
MseI TTAA 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 652
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 4 cut(s) 67, 117, 593, 602
NdeII GATC 6 cut(s) 52, 73, 79, 165, 253, 693
NlaIV GGNNCC 1 cut(s) 623
NmuCI GTSAC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 2 cut(s) 530, 574
PfeI GAWTC 2 cut(s) 134, 713
PkrI GCNGC 6 cut(s) 63, 498, 557, 589, 607, 651
PleI GAGTC 2 cut(s) 515, 676
PmaCI CACGTG 2 cut(s) 107, 569
PmlI CACGTG 2 cut(s) 107, 569
PpsI GAGTC 2 cut(s) 515, 676
Ppu21I YACGTR 3 cut(s) 107, 197, 569
PsiI TTATAA 1 cut(s) 446
PspCI CACGTG 2 cut(s) 107, 569
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 1 cut(s) 84
PstI CTGCAG 2 cut(s) 66, 498
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SalI GTCGAC 1 cut(s) 532
SaqAI TTAA 1 cut(s) 659
SatI GCNGC 6 cut(s) 62, 497, 556, 588, 606, 650
Sau3AI GATC 6 cut(s) 52, 73, 79, 165, 253, 693
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 2 cut(s) 515, 677
SduI GDGCHC 3 cut(s) 202, 340, 644
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 2 cut(s) 62, 494
SinI GGWCC 1 cut(s) 84
SsiI CCGC 4 cut(s) 128, 539, 555, 652
SspMI CTAG 3 cut(s) 347, 393, 402
StyI CCWWGG 2 cut(s) 392, 559
TaaI ACNGT 3 cut(s) 213, 322, 639
TaiI ACGT 4 cut(s) 109, 199, 571, 603
TaqI TCGA 5 cut(s) 164, 330, 489, 533, 671
TaqII GACCGA 3 cut(s) 101, 421, 720
TauI GCSGC 1 cut(s) 558
TfiI GAWTC 2 cut(s) 134, 713
Tru1I TTAA 1 cut(s) 659
Tru9I TTAA 1 cut(s) 659
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 5 cut(s) 61, 496, 587, 605, 649
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 5 cut(s) 17, 147, 224, 372, 408
TspRI CASTG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmaJI CCTAGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 533
XspI CTAG 3 cut(s) 347, 393, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.