Rroxscaffold_159G00433110

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0003779
Physical Location & Seq
Reverse (-)
865928 .. 872737
6810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00433110.1

Sequence Viewer

Length: 609 bp
ATGATCGATTCAACAAGACCCACTTGTGTTAGACCGAGCTCTAGTTCATCCTTGTCCGAAACCCCGCTTCTTTTAGTTGCAATGTTGGGCCATTTGGACTTGGTAAAAACGCTATCGAATTGCAAGCCCGAGTTCGCCACCGAGTTGGACTCGCACGGGTCGACCCCGCTCCACCCGACGGCGGCCAAGGGACATGTGGGGGTCGTGAGGGAGCTGCTTTGGGCCGACGTGGCTGCGTGTTTGGTGAGGAATCGAGAATGGGAGGACGGCGCTCCACAAATAAATAATTTTTTTGAAAAATATGAACTCGATCGACTACGGTTCATAGAGCAACGGCTCACGGTGCCTCACTTGGCTGCTATGTTGGATACCGGTAGGACGCCGGGTCCGAGATCCGTGTCAAGAATGGGGCAAGTAGGTTGTGCGGCTGGCCAAAAGAGTTTCAAGAAAGGGCGTTGTACGGCGGGTTGGAAGAGTGGGGGAAATCGGGCTGCACTTGCTAAGCAAGGCGGGGCTCGATCAATCTGCGATGGGATAGTGGAGGTGAGGCCGGGATCGAGATCGCTCCGATTTGGGTTGGTTGCAAGCGAAGGGCCGGCGGATCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

202

Amino Acids

21.69

Weight (kDa)

9.19

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 73 1.1e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 161
AciI CCGC 7 cut(s) 65, 167, 182, 425, 464, 510, 599
AclWI GGATC 3 cut(s) 387, 562, 596
AcoI YGGCCR 2 cut(s) 183, 430
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 178, 181
AflIII ACRYGT 1 cut(s) 193
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 3 cut(s) 12, 296, 445
AhdI GACNNNNNGTC 1 cut(s) 384
AjiI CACGTC 1 cut(s) 229
AluBI AGCT 2 cut(s) 39, 214
AluI AGCT 2 cut(s) 39, 214
Alw21I GWGCWC 1 cut(s) 41
AlwI GGATC 3 cut(s) 387, 562, 596
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 6 cut(s) 88, 183, 222, 430, 548, 593
ApeKI GCWGC 4 cut(s) 214, 233, 356, 491
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 4 cut(s) 88, 222, 386, 593
AsuC2I CCSGG 2 cut(s) 384, 552
AsuHPI GGTGA 2 cut(s) 256, 556
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 386
BalI TGGCCA 1 cut(s) 432
BamHI GGATCC 1 cut(s) 601
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 2 cut(s) 41, 517
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 201, 220, 343, 478
BccI CCATC 1 cut(s) 524
BceAI ACGGC 4 cut(s) 195, 283, 350, 477
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 507, 541
BciVI GTATCC 1 cut(s) 361
BcnI CCSGG 2 cut(s) 384, 552
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 273
BfuI GTATCC 1 cut(s) 361
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 6 cut(s) 183, 215, 234, 357, 426, 492
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 6 cut(s) 184, 216, 235, 358, 427, 493
Bme1390I CCNGG 2 cut(s) 384, 552
Bme18I GGWCC 1 cut(s) 386
BmeRI GACNNNNNGTC 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 4 cut(s) 88, 222, 386, 593
BmiI GGNNCC 3 cut(s) 345, 387, 603
BmrFI CCNGG 2 cut(s) 384, 552
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu1102I GCTNAGC 1 cut(s) 501
BpuMI CCSGG 2 cut(s) 384, 552
Bsa29I ATCGAT 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 559
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 181
Bse118I RCCGGY 2 cut(s) 371, 595
Bse3DI GCAATG 1 cut(s) 87
Bse8I GATNNNNATC 1 cut(s) 559
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 47
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 2 cut(s) 178, 181
BseMI GCAATG 1 cut(s) 87
BseXI GCAGC 4 cut(s) 201, 220, 343, 478
BsgI GTGCAG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 6 cut(s) 90, 185, 224, 432, 550, 595
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 4 cut(s) 372, 383, 551, 596
BslFI GGGAC 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 178, 181
BsmFI GGGAC 1 cut(s) 204
BsnI GGCC 6 cut(s) 90, 185, 224, 432, 550, 595
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 41, 517
Bsp143I GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
Bsp1720I GCTNAGC 1 cut(s) 501
BspACI CCGC 7 cut(s) 65, 167, 182, 425, 464, 510, 599
BspANI GGCC 6 cut(s) 90, 185, 224, 432, 550, 595
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 3 cut(s) 345, 387, 603
BspPI GGATC 3 cut(s) 387, 562, 596
BspT107I GGYRCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 2 cut(s) 371, 595
BssAI RCCGGY 2 cut(s) 371, 595
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 321, 343
Bst6I CTCTTC 1 cut(s) 467
BstACI GRCGYC 1 cut(s) 380
BstC8I GCNNGC 4 cut(s) 125, 430, 586, 597
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 7 cut(s) 6, 313, 395, 521, 557, 563, 604
BstMBI GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 3 cut(s) 230, 343, 497
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 382, 550
BstV1I GCAGC 4 cut(s) 201, 220, 343, 478
BstX2I RGATCY 2 cut(s) 392, 601
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 392, 601
Bsu15I ATCGAT 1 cut(s) 6
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 6 cut(s) 90, 185, 224, 432, 550, 595
BsuTUI ATCGAT 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 47
Cac8I GCNNGC 4 cut(s) 125, 430, 586, 597
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 371, 595
Cfr13I GGNCC 4 cut(s) 88, 222, 386, 593
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 459
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 1 cut(s) 194
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 501
DpnI GATC 7 cut(s) 5, 312, 394, 520, 556, 562, 603
DpnII GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
DriI GACNNNNNGTC 1 cut(s) 384
EaeI YGGCCR 2 cut(s) 183, 430
Eam1104I CTCTTC 1 cut(s) 467
Eam1105I GACNNNNNGTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 467
Ecl136II GAGCTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 41, 517
Eco47I GGWCC 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 41, 517
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 197
FaiI YATR 4 cut(s) 195, 303, 326, 362
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 204
FatI CATG 1 cut(s) 193
FauI CCCGC 4 cut(s) 72, 174, 457, 503
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 6 cut(s) 183, 215, 234, 357, 426, 492
FokI GGATG 1 cut(s) 34
FriOI GRGCYC 2 cut(s) 41, 517
Fsp4HI GCNGC 6 cut(s) 183, 215, 234, 357, 426, 492
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 271
GluI GCNGC 6 cut(s) 183, 215, 234, 357, 426, 492
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 6 cut(s) 90, 185, 224, 432, 550, 595
HapII CCGG 4 cut(s) 372, 383, 551, 596
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 3 cut(s) 8, 149, 250
HpaII CCGG 4 cut(s) 372, 383, 551, 596
HphI GGTGA 2 cut(s) 256, 556
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 3 cut(s) 58, 390, 569
Hpy188III TCNNGA 5 cut(s) 205, 254, 402, 445, 558
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 2 cut(s) 181, 230
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 2 cut(s) 321, 343
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 4 cut(s) 80, 123, 494, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 343, 497
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 270
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
Kzo9I GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
LmnI GCTCC 4 cut(s) 174, 211, 277, 570
LpnPI CCDG 4 cut(s) 385, 396, 414, 564
Lsp1109I GCAGC 4 cut(s) 201, 220, 343, 478
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 228
MalI GATC 7 cut(s) 5, 312, 394, 520, 556, 562, 603
MbiI CCGCTC 1 cut(s) 169
MboI GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
MboII GAAGA 1 cut(s) 484
MflI RGATCY 2 cut(s) 392, 601
MhlI GDGCHC 2 cut(s) 41, 517
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 2 cut(s) 118, 286
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 3 cut(s) 126, 345, 449
MnlI CCTC 6 cut(s) 201, 240, 256, 357, 535, 540
Mox20I TGGCCA 1 cut(s) 432
MroNI GCCGGC 1 cut(s) 595
MscI TGGCCA 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 4 cut(s) 372, 383, 551, 596
MspR9I CCNGG 2 cut(s) 384, 552
MwoI GCNNNNNNNGC 3 cut(s) 230, 343, 497
NaeI GCCGGC 1 cut(s) 597
NciI CCSGG 2 cut(s) 384, 552
NdeII GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 3 cut(s) 345, 387, 603
NspI RCATGY 1 cut(s) 197
PciI ACATGT 1 cut(s) 193
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 386
PdiI GCCGGC 1 cut(s) 597
PfeI GAWTC 2 cut(s) 8, 250
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 6 cut(s) 184, 216, 235, 358, 427, 493
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PscI ACATGT 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 41
PspN4I GGNNCC 3 cut(s) 345, 387, 603
PspPI GGNCC 4 cut(s) 88, 222, 386, 593
PsuI RGATCY 2 cut(s) 392, 601
PvuI CGATCG 1 cut(s) 313
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SacI GAGCTC 1 cut(s) 41
SalI GTCGAC 1 cut(s) 160
SatI GCNGC 6 cut(s) 183, 215, 234, 357, 426, 492
Sau3AI GATC 7 cut(s) 3, 310, 392, 518, 554, 560, 601
Sau96I GGNCC 4 cut(s) 88, 222, 386, 593
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 384, 552
SduI GDGCHC 2 cut(s) 41, 517
SetI ASST 5 cut(s) 41, 216, 231, 421, 546
SinI GGWCC 1 cut(s) 386
Sse9I AATT 2 cut(s) 118, 286
SsiI CCGC 7 cut(s) 65, 167, 182, 425, 464, 510, 599
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 382, 550
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 321, 343
TaiI ACGT 1 cut(s) 231
TaqI TCGA 8 cut(s) 6, 116, 161, 253, 309, 313, 517, 557
TaqII GACCGA 1 cut(s) 49
TasI AATT 2 cut(s) 118, 286
TauI GCSGC 2 cut(s) 185, 428
TfiI GAWTC 2 cut(s) 8, 250
TseI GCWGC 4 cut(s) 214, 233, 356, 491
TspDTI ATGAA 3 cut(s) 36, 313, 318
TspGWI ACGGA 2 cut(s) 385, 594
VpaK11BI GGWCC 1 cut(s) 386
XceI RCATGY 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 161
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.