Rroxscaffold_4G00325080

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003779
Physical Location & Seq
Forward (+)
56816883 .. 56821095
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325080.1

Sequence Viewer

Length: 402 bp
ATGATCGATTCAACAAGACCCACTTGTGTTAGACCGAGCTCTAGTTCATCCTTGTCCGAAACCCCGCTTCTTTTAGTTGCAATGTTGGGCCATTTGGACTTGGTAAAAACGCTATCGAATTGCAAGCCCGAGTTCGCCACCGAGTTGGACTCGCACGGGTCGACCCCGCTCCACCCGACGGCGGCCAAGGGACATGTGGGGGTCGTGAGGGAGCTGCTTTGGGCCGACGTGGCTGCGTGTTTGGTGAGGAATCGAGAATGGGAGGACGGCGCTCCACGTAGCAGGCTGTTAAAGGAAGAGTCGAGGTGGTGGGGGGGAAGTTGGTCCGAGCCGGGACCGAACTATCCTCCTACCATGAGGGGGAGAAAGATTGTTCCTGAAGAACGACAAGAATTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

133

Amino Acids

14.8

Weight (kDa)

6.42

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 73 4.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 161
AciI CCGC 3 cut(s) 65, 167, 182
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 399
AfiI CCNNNNNNNGG 3 cut(s) 178, 181, 360
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 1 cut(s) 12
AjiI CACGTC 1 cut(s) 229
AluBI AGCT 2 cut(s) 39, 214
AluI AGCT 2 cut(s) 39, 214
Alw21I GWGCWC 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 88, 183, 222
ApeKI GCWGC 2 cut(s) 214, 233
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 4 cut(s) 88, 222, 324, 335
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 324, 335
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 201, 220
BceAI ACGGC 2 cut(s) 195, 283
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BcnI CCSGG 1 cut(s) 333
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 3 cut(s) 183, 215, 234
BlsI GCNGC 3 cut(s) 184, 216, 235
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 324, 335
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 4 cut(s) 88, 222, 324, 335
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpuMI CCSGG 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 181, 360
Bse3DI GCAATG 1 cut(s) 87
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 3 cut(s) 178, 181, 360
BseMI GCAATG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 201, 220
BshFI GGCC 3 cut(s) 90, 185, 224
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 2 cut(s) 204, 348
BslI CCNNNNNNNGG 3 cut(s) 178, 181, 360
BsmFI GGGAC 2 cut(s) 204, 348
BsnI GGCC 3 cut(s) 90, 185, 224
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 3 cut(s) 65, 167, 182
BspANI GGCC 3 cut(s) 90, 185, 224
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 336
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 125, 284
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 201, 220
BstXI CCANNNNNNTGG 1 cut(s) 145
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 3 cut(s) 90, 185, 224
BsuTUI ATCGAT 1 cut(s) 6
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 125, 284
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 4 cut(s) 88, 222, 324, 335
ClaI ATCGAT 1 cut(s) 6
CviAII CATG 2 cut(s) 194, 355
CviJI RGCY 9 cut(s) 39, 90, 127, 185, 214, 224, 233, 286, 331
CviKI_1 RGCY 9 cut(s) 39, 90, 127, 185, 214, 224, 233, 286, 331
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 324, 335
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 197, 358
FaiI YATR 2 cut(s) 195, 356
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 2 cut(s) 204, 348
FatI CATG 2 cut(s) 193, 354
FauI CCCGC 2 cut(s) 72, 174
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 183, 215, 234
FokI GGATG 1 cut(s) 34
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 183, 215, 234
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 271
GluI GCNGC 3 cut(s) 183, 215, 234
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 3 cut(s) 90, 185, 224
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 197, 358
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 4 cut(s) 8, 149, 250, 299
HpaII CCGG 1 cut(s) 332
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 58, 328
Hpy188III TCNNGA 3 cut(s) 205, 254, 377
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 2 cut(s) 181, 230
HpyCH4IV ACGT 2 cut(s) 228, 277
HpyCH4V TGCA 2 cut(s) 80, 123
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 228, 277
Hsp92II CATG 2 cut(s) 197, 358
HspAI GCGC 1 cut(s) 270
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 3 cut(s) 174, 211, 277
LpnPI CCDG 3 cut(s) 268, 345, 390
Lsp1109I GCAGC 2 cut(s) 201, 220
MaeI CTAG 1 cut(s) 42
MaeII ACGT 2 cut(s) 228, 277
MalI GATC 1 cut(s) 5
MbiI CCGCTC 1 cut(s) 169
MboI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 308, 392
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 2 cut(s) 118, 392
MlyI GAGTC 2 cut(s) 143, 308
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 6 cut(s) 201, 240, 256, 297, 351, 357
MseI TTAA 1 cut(s) 290
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 230
NciI CCSGG 1 cut(s) 333
NdeII GATC 1 cut(s) 3
NlaIII CATG 2 cut(s) 197, 358
NlaIV GGNNCC 1 cut(s) 336
NspI RCATGY 1 cut(s) 197
PciI ACATGT 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 2 cut(s) 8, 250
PkrI GCNGC 3 cut(s) 184, 216, 235
PleI GAGTC 2 cut(s) 143, 307
PpsI GAGTC 2 cut(s) 143, 307
Ppu21I YACGTR 1 cut(s) 278
PscI ACATGT 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 4 cut(s) 88, 222, 324, 335
SacI GAGCTC 1 cut(s) 41
SalI GTCGAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 3 cut(s) 183, 215, 234
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 4 cut(s) 88, 222, 324, 335
SchI GAGTC 2 cut(s) 143, 308
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 41
SetI ASST 5 cut(s) 41, 216, 231, 280, 308
SinI GGWCC 2 cut(s) 324, 335
Sse9I AATT 2 cut(s) 118, 392
SsiI CCGC 3 cut(s) 65, 167, 182
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 2 cut(s) 231, 280
TaqI TCGA 5 cut(s) 6, 116, 161, 253, 302
TaqII GACCGA 2 cut(s) 49, 352
TasI AATT 2 cut(s) 118, 392
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 8, 250
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TseI GCWGC 2 cut(s) 214, 233
TspDTI ATGAA 1 cut(s) 36
VpaK11BI GGWCC 2 cut(s) 324, 335
XceI RCATGY 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 161
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.