Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002352

Physical Location & Seq

Genomic Coordinates Reverse (-)

24114855 .. 24116177

Gene Structure 1323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1236900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGGCCTCCTCTTTCTTTTTCTCAAGCCCACCACCCCCGCCACCTTCTTCCTCCTCCTCCTCCCCACCCTCCTCCTCTCTTTTCTCATCAAAACCACTCGCGCGCACCCACACGCAGCAGCAGCAGATTCCCATCTCTCCACCCCCGGCTTCGGCGACATTGCCAGCTCTGTCAACCCATCTCCCTCACCGCCCCATCCTCCTCAACCCAACAACGCGGGCCCCACCAGATCCTCCTCTCTCCGGCCGAGCATAGCCATAATCGTCGCCGTTCTCACCACCGTCTTCTCAGTCGTCTTCCTTATTCTCCTCTACGCCAAGCACTGCAAGCGCGGTGCTCTAGTTATCGTCGGCTCTAACTCCAATTCCGGACCCACCGGACCCGCCCCACGTAAAAACTCAGGCATTGACCGCTCCGTCGTCGAGTCCCTACCAGTGTTCCGCTTCAGGTCCCTTCGCGGCCATAAGGACGGGCTCGAGTGCGCGGTCTGCCTGACCCGGTTCGACCAAACCGAGGTCCTCCGTCTCCTGCCAAAATGCAAACACGCGTTCCACGTCGAGTGCGTCGATACGTGGCTCGACGCCCACTCCACCTGCCCCCTCTGCCGGTACCGGGTCGACCCCGAAGACGTCCTCTTAGTCGAGGACGCCGCAAAGCTCTTGCATCCGAAGAATAGCGATGTCGTTTTGGAGATCGAGAACGACCCGGTTTTTCGTCGGATTTCGGGGCGGCACTCGTTCGCCGGAGAACACAGACCGGGGCATCATCAAGCCCAAACGTGGTCGTTTCGGAGGTCGCTGGACAGCTGGACAGCAATCCGAATGAAGAGCGAGCCGGCCACCGTCGGATGCTTCGACCGACCCAGAAAAGATGGACTCCTGTTGGGCCAAGACAGGACGAGGTTGGAGCACCGGATCATCGTGTCCCCTACCGGGTCGGGGTCTGGTCCCGGTGGGCTCCAGCAGAGATGGAGCGACGTACTGCCGTCGGATCTTCTGTATCTACGGTCGGAAATGATAATGAGCGAGGGCTTGACACGTCAGCGGGCGCAGCAGAATGAGAATAGTAATGGGCAGCAGGCGAGTGCGGGGCCCGCGGCAGGAGGAGGGCCCGGAGGGATTGATGGGAAGAGGAGATCATGGAGCTGTGGTGGTTCGAACAGAGCGACGGGGGGCAATGAGAGCCAACAGGAACAACAACAACAACGACAGCTCAAGCTTGTGGCCAGGTGGCTGGCCTGGGTTTCTTCTCTCTCGCGACCGGCAATACGGACGGAGCGTACTACTATTCCCACCCCCACTACTACACCGCCCATTTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

441

Amino Acids

48.07

Weight (kDa)

9.68

Isoelectric Point (pI)

55.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	160 - 203	1.5e-13	Ring finger domain
zf-RING_11	PF17123	160 - 189	6.7e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	160 - 202	3.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	161 - 202	1.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	166 - 203	2.7e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05810
fragaria_vesca	FvH4_4g06660
malus_domestica	MD13G1236900.v1.1 MD16G1242200.v1.1
prunus_persica	Prupe.1G069300_v2.0.a1
pyrus_communis	pycom13g20980 pycom16g20300
rosa_chinensis	RchiOBHm_Chr3g0495141 RchiOBHm_Chr4g0398561
rosa_laevigata	RLG00000009344 RLG00000026064 RLG00000029692 RLG00000030599 RLG00000032695 RLG00000032696
rosa_multiflora	Rmu_co8139468.1_g000001 Rmu_co8270033.1_g000001 Rmu_co8275827.1_g000001 Rmu_sc0000185.1_g000029 Rmu_sc0000990.1_g000016 Rmu_sc0005217.1_g000007 Rmu_sc0005996.1_g000027 Rmu_sc0009001.1_g000015 Rmu_sc0013727.1_g000001 Rmu_sc0016350.1_g000001
rosa_roxburghii	Rroxscaffold_5G00343530
rosa_rugosa	Rorug04G0009400
rosa_samantha	Rh1AG207300 Rh2AG231000 Rh2AG449700 Rh2CG436600 Rh2DG238800 Rh3DG271300 Rh4AG087300 Rh4BG083900 Rh4CG095500 Rh4DG078800 Rh4DG124400 Rh5DG085200 Rh6BG230400
rosa_wichuraiana	Rw1G018260 Rw1G018370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	602
AasI	GACNNNNNNGTC	1 cut(s)	416
AatII	GACGTC	1 cut(s)	633
Acc36I	ACCTGC	1 cut(s)	602
Acc65I	GGTACC	1 cut(s)	609
AccB1I	GGYRCC	1 cut(s)	609
AccBSI	CCGCTC	1 cut(s)	414
AccI	GTMKAC	1 cut(s)	618
AccII	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
AccIII	TCCGGA	1 cut(s)	368
AclWI	GGATC	3 cut(s)	225, 923, 999
AcoI	YGGCCR	4 cut(s)	245, 460, 837, 1224
AcuI	CTGAAG	1 cut(s)	430
AcyI	GRCGYC	3 cut(s)	582, 630, 648
AfaI	GTAC	3 cut(s)	611, 981, 1282
AfiI	CCNNNNNNNGG	9 cut(s)	152, 243, 514, 606, 613, 780, 933, 939, 1100
AflIII	ACRYGT	2 cut(s)	546, 1037
AjiI	CACGTC	2 cut(s)	556, 1040
AjnI	CCWGG	2 cut(s)	1226, 1238
AluBI	AGCT	6 cut(s)	168, 658, 807, 1146, 1213, 1219
AluI	AGCT	6 cut(s)	168, 658, 807, 1146, 1213, 1219
Alw21I	GWGCWC	2 cut(s)	340, 912
Alw26I	GTCTC	1 cut(s)	530
AlwI	GGATC	3 cut(s)	225, 923, 999
Ama87I	CYCGRG	1 cut(s)	476
Aor13HI	TCCGGA	1 cut(s)	368
ApaI	GGGCCC	3 cut(s)	224, 1095, 1113
ApeKI	GCWGC	5 cut(s)	116, 119, 122, 1051, 1075
Asp718I	GGTACC	1 cut(s)	609
AspLEI	GCGC	5 cut(s)	104, 106, 333, 485, 1051
AsuC2I	CCSGG	8 cut(s)	147, 499, 614, 707, 759, 934, 951, 1113
AsuHPI	GGTGA	2 cut(s)	180, 268
AsuII	TTCGAA	1 cut(s)	1157
AvaI	CYCGRG	1 cut(s)	476
AvaII	GGWCC	5 cut(s)	371, 380, 450, 517, 947
BaeGI	GKGCMC	3 cut(s)	224, 1095, 1113
BalI	TGGCCA	1 cut(s)	1226
BanI	GGYRCC	1 cut(s)	609
BanII	GRGCYC	5 cut(s)	224, 477, 960, 1095, 1113
BbsI	GAAGAC	3 cut(s)	277, 289, 633
Bbv12I	GWGCWC	2 cut(s)	340, 912
BbvI	GCAGC	5 cut(s)	128, 131, 134, 1063, 1087
BccI	CCATC	6 cut(s)	141, 187, 204, 866, 963, 1118
BceAI	ACGGC	2 cut(s)	254, 970
BciT130I	CCWGG	2 cut(s)	1228, 1240
BcnI	CCSGG	8 cut(s)	147, 499, 614, 707, 759, 934, 951, 1113
BcoDI	GTCTC	1 cut(s)	530
BfaI	CTAG	2 cut(s)	341, 1321
BfuAI	ACCTGC	1 cut(s)	602
BisI	GCNGC	9 cut(s)	117, 120, 123, 460, 651, 731, 1052, 1076, 1098
BlsI	GCNGC	9 cut(s)	118, 121, 124, 461, 652, 732, 1053, 1077, 1099
Bme18I	GGWCC	5 cut(s)	371, 380, 450, 517, 947
BmeT110I	CYCGRG	1 cut(s)	476
BmgBI	CACGTC	2 cut(s)	556, 1040
BmsI	GCATC	3 cut(s)	673, 772, 839
BpiI	GAAGAC	3 cut(s)	277, 289, 633
BpmI	CTGGAG	1 cut(s)	944
Bpu14I	TTCGAA	1 cut(s)	1157
BpuEI	CTTGAG	2 cut(s)	8, 1199
BpuMI	CCSGG	8 cut(s)	147, 499, 614, 707, 759, 934, 951, 1113
BsaAI	YACGTR	2 cut(s)	392, 573
BsaBI	GATNNNNATC	1 cut(s)	132
BsaHI	GRCGYC	3 cut(s)	582, 630, 648
BsaJI	CCNNGG	5 cut(s)	145, 513, 758, 1095, 1239
BsaWI	WCCGGW	3 cut(s)	368, 377, 912
BsaXI	ACNNNNNCTCC	4 cut(s)	572, 602, 1135, 1165
Bsc4I	CCNNNNNNNGG	9 cut(s)	152, 243, 514, 606, 613, 780, 933, 939, 1100
Bse118I	RCCGGY	3 cut(s)	606, 835, 1261
Bse1I	ACTGG	1 cut(s)	434
Bse3DI	GCAATG	2 cut(s)	159, 1183
Bse8I	GATNNNNATC	1 cut(s)	132
BseAI	TCCGGA	1 cut(s)	368
BseBI	CCWGG	2 cut(s)	1228, 1240
BseDI	CCNNGG	5 cut(s)	145, 513, 758, 1095, 1239
BseGI	GGATG	3 cut(s)	196, 664, 854
BseJI	GATNNNNATC	1 cut(s)	132
BseLI	CCNNNNNNNGG	9 cut(s)	152, 243, 514, 606, 613, 780, 933, 939, 1100
BseMI	GCAATG	2 cut(s)	159, 1183
BseMII	CTCAG	2 cut(s)	303, 414
BseNI	ACTGG	1 cut(s)	434
BsePI	GCGCGC	1 cut(s)	102
BseSI	GKGCMC	3 cut(s)	224, 1095, 1113
BseX3I	CGGCCG	1 cut(s)	245
BseXI	GCAGC	5 cut(s)	128, 131, 134, 1063, 1087
Bsh1236I	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
Bsh1285I	CGRYCG	4 cut(s)	248, 859, 1010, 1262
BshNI	GGYRCC	1 cut(s)	609
BsiEI	CGRYCG	4 cut(s)	248, 859, 1010, 1262
BsiHKAI	GWGCWC	2 cut(s)	340, 912
BsiHKCI	CYCGRG	1 cut(s)	476
BslFI	GGGAC	4 cut(s)	412, 436, 910, 933
BslI	CCNNNNNNNGG	9 cut(s)	152, 243, 514, 606, 613, 780, 933, 939, 1100
BsmAI	GTCTC	1 cut(s)	530
BsmBI	CGTCTC	1 cut(s)	530
BsmFI	GGGAC	4 cut(s)	412, 436, 910, 933
BsoBI	CYCGRG	1 cut(s)	476
Bsp119I	TTCGAA	1 cut(s)	1157
Bsp120I	GGGCCC	3 cut(s)	220, 1091, 1109
Bsp1286I	GDGCHC	7 cut(s)	224, 340, 477, 912, 960, 1095, 1113
Bsp13I	TCCGGA	1 cut(s)	368
Bsp143I	GATC	5 cut(s)	230, 693, 915, 991, 1136
Bsp68I	TCGCGA	1 cut(s)	1258
BspCNI	CTCAG	2 cut(s)	302, 413
BspEI	TCCGGA	1 cut(s)	368
BspFNI	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
BspMI	ACCTGC	1 cut(s)	602
BspPI	GGATC	3 cut(s)	225, 923, 999
BspQI	GCTCTTC	1 cut(s)	821
BspT104I	TTCGAA	1 cut(s)	1157
BspT107I	GGYRCC	1 cut(s)	609
BsrBI	CCGCTC	1 cut(s)	414
BsrDI	GCAATG	2 cut(s)	159, 1183
BsrFI	RCCGGY	3 cut(s)	606, 835, 1261
BsrI	ACTGG	1 cut(s)	434
BssAI	RCCGGY	3 cut(s)	606, 835, 1261
BssECI	CCNNGG	5 cut(s)	145, 513, 758, 1095, 1239
BssHII	GCGCGC	1 cut(s)	102
BssMI	GATC	5 cut(s)	230, 693, 915, 991, 1136
BssNI	GRCGYC	3 cut(s)	582, 630, 648
Bst2UI	CCWGG	2 cut(s)	1228, 1240
Bst4CI	ACNGT	3 cut(s)	283, 844, 1008
Bst6I	CTCTTC	2 cut(s)	821, 1124
BstACI	GRCGYC	3 cut(s)	582, 630, 648
BstBAI	YACGTR	2 cut(s)	392, 573
BstBI	TTCGAA	1 cut(s)	1157
BstDEI	CTNAG	3 cut(s)	289, 400, 637
BstDSI	CCRYGG	1 cut(s)	1095
BstF5I	GGATG	3 cut(s)	196, 664, 854
BstFNI	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
BstHHI	GCGC	5 cut(s)	104, 106, 333, 485, 1051
BstKTI	GATC	5 cut(s)	233, 696, 918, 994, 1139
BstMAI	GTCTC	1 cut(s)	530
BstMBI	GATC	5 cut(s)	230, 693, 915, 991, 1136
BstMCI	CGRYCG	4 cut(s)	248, 859, 1010, 1262
BstMWI	GCNNNNNNNGC	8 cut(s)	122, 328, 411, 489, 603, 1051, 1094, 1182
BstNI	CCWGG	2 cut(s)	1228, 1240
BstSLI	GKGCMC	3 cut(s)	224, 1095, 1113
BstUI	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
BstV1I	GCAGC	5 cut(s)	128, 131, 134, 1063, 1087
BstV2I	GAAGAC	3 cut(s)	277, 289, 633
BstX2I	RGATCY	2 cut(s)	230, 991
BstXI	CCANNNNNNTGG	1 cut(s)	1234
BstYI	RGATCY	2 cut(s)	230, 991
BstZI	CGGCCG	1 cut(s)	245
BtgI	CCRYGG	1 cut(s)	1095
BtgZI	GCGATG	1 cut(s)	693
BtrI	CACGTC	2 cut(s)	556, 1040
BtsCI	GGATG	3 cut(s)	196, 664, 854
BtsI	GCAGTG	1 cut(s)	322
BtsIMutI	CAGTG	2 cut(s)	322, 441
BtuMI	TCGCGA	1 cut(s)	1258
BveI	ACCTGC	1 cut(s)	602
CfoI	GCGC	5 cut(s)	104, 106, 333, 485, 1051
Cfr10I	RCCGGY	3 cut(s)	606, 835, 1261
Cfr42I	CCGCGG	1 cut(s)	1098
CseI	GACGC	3 cut(s)	553, 590, 656
Csp6I	GTAC	3 cut(s)	610, 980, 1281
CviAII	CATG	1 cut(s)	1140
CviQI	GTAC	3 cut(s)	610, 980, 1281
DdeI	CTNAG	3 cut(s)	289, 400, 637
DpnI	GATC	5 cut(s)	232, 695, 917, 993, 1138
DpnII	GATC	5 cut(s)	230, 693, 915, 991, 1136
DrdI	GACNNNNNNGTC	1 cut(s)	416
DseDI	GACNNNNNNGTC	1 cut(s)	416
EaeI	YGGCCR	4 cut(s)	245, 460, 837, 1224
EagI	CGGCCG	1 cut(s)	245
Eam1104I	CTCTTC	2 cut(s)	821, 1124
EarI	CTCTTC	2 cut(s)	821, 1124
EclXI	CGGCCG	1 cut(s)	245
Eco24I	GRGCYC	5 cut(s)	224, 477, 960, 1095, 1113
Eco47I	GGWCC	5 cut(s)	371, 380, 450, 517, 947
Eco52I	CGGCCG	1 cut(s)	245
Eco57I	CTGAAG	1 cut(s)	430
Eco88I	CYCGRG	1 cut(s)	476
EcoO109I	RGGNCCY	5 cut(s)	221, 450, 517, 1091, 1109
EcoRII	CCWGG	2 cut(s)	1226, 1238
EcoT38I	GRGCYC	5 cut(s)	224, 477, 960, 1095, 1113
Esp3I	CGTCTC	1 cut(s)	530
FaeI	CATG	1 cut(s)	1143
FaiI	YATR	4 cut(s)	254, 260, 465, 1141
FaqI	GGGAC	4 cut(s)	412, 436, 910, 933
FatI	CATG	1 cut(s)	1139
FauI	CCCGC	6 cut(s)	46, 211, 391, 1038, 1081, 1102
FblI	GTMKAC	1 cut(s)	618
Fnu4HI	GCNGC	9 cut(s)	117, 120, 123, 460, 651, 731, 1052, 1076, 1098
FokI	GGATG	3 cut(s)	183, 651, 861
FriOI	GRGCYC	5 cut(s)	224, 477, 960, 1095, 1113
Fsp4HI	GCNGC	9 cut(s)	117, 120, 123, 460, 651, 731, 1052, 1076, 1098
FspBI	CTAG	2 cut(s)	341, 1321
GlaI	GCGC	5 cut(s)	103, 105, 332, 484, 1050
GluI	GCNGC	9 cut(s)	117, 120, 123, 460, 651, 731, 1052, 1076, 1098
GsuI	CTGGAG	1 cut(s)	944
HgaI	GACGC	3 cut(s)	553, 590, 656
HhaI	GCGC	5 cut(s)	104, 106, 333, 485, 1051
Hin1I	GRCGYC	3 cut(s)	582, 630, 648
Hin1II	CATG	1 cut(s)	1143
Hin6I	GCGC	5 cut(s)	102, 104, 331, 483, 1049
HinP1I	GCGC	5 cut(s)	102, 104, 331, 483, 1049
HincII	GTYRAC	2 cut(s)	175, 619
HindII	GTYRAC	2 cut(s)	175, 619
HindIII	AAGCTT	1 cut(s)	1217
HinfI	GANTC	3 cut(s)	128, 425, 876
HphI	GGTGA	2 cut(s)	180, 268
Hpy166II	GTNNAC	2 cut(s)	175, 619
Hpy188I	TCNGA	7 cut(s)	669, 720, 792, 821, 848, 991, 1012
Hpy188III	TCNNGA	3 cut(s)	369, 697, 1257
Hpy8I	GTNNAC	2 cut(s)	175, 619
HpyAV	CCTTC	2 cut(s)	55, 464
HpyCH4III	ACNGT	3 cut(s)	283, 844, 1008
HpyCH4IV	ACGT	7 cut(s)	391, 555, 572, 630, 779, 978, 1039
HpyCH4V	TGCA	3 cut(s)	327, 540, 664
HpyF10VI	GCNNNNNNNGC	8 cut(s)	122, 328, 411, 489, 603, 1051, 1094, 1182
HpyF3I	CTNAG	3 cut(s)	289, 400, 637
HpySE526I	ACGT	7 cut(s)	391, 555, 572, 630, 779, 978, 1039
Hsp92I	GRCGYC	3 cut(s)	582, 630, 648
Hsp92II	CATG	1 cut(s)	1143
HspAI	GCGC	5 cut(s)	102, 104, 331, 483, 1049
Kpn2I	TCCGGA	1 cut(s)	368
KpnI	GGTACC	1 cut(s)	613
KroI	GCCGGC	1 cut(s)	835
KroNI	GCCGGC	1 cut(s)	837
KspI	CCGCGG	1 cut(s)	1098
Kzo9I	GATC	5 cut(s)	230, 693, 915, 991, 1136
LguI	GCTCTTC	1 cut(s)	821
LmnI	GCTCC	6 cut(s)	419, 907, 963, 972, 1143, 1276
Lsp1109I	GCAGC	5 cut(s)	128, 131, 134, 1063, 1087
LweI	GCATC	3 cut(s)	673, 772, 839
MaeI	CTAG	2 cut(s)	341, 1321
MaeII	ACGT	7 cut(s)	391, 555, 572, 630, 779, 978, 1039
MalI	GATC	5 cut(s)	232, 695, 917, 993, 1138
MbiI	CCGCTC	1 cut(s)	414
MboI	GATC	5 cut(s)	230, 693, 915, 991, 1136
MboII	GAAGA	9 cut(s)	40, 277, 289, 638, 682, 838, 986, 1141, 1239
MflI	RGATCY	2 cut(s)	230, 991
MhlI	GDGCHC	7 cut(s)	224, 340, 477, 912, 960, 1095, 1113
MlsI	TGGCCA	1 cut(s)	1226
MluCI	AATT	1 cut(s)	364
MluI	ACGCGT	1 cut(s)	546
MluNI	TGGCCA	1 cut(s)	1226
MlyI	GAGTC	2 cut(s)	434, 870
MmeI	TCCRAC	5 cut(s)	698, 826, 885, 969, 990
Mox20I	TGGCCA	1 cut(s)	1226
MroI	TCCGGA	1 cut(s)	368
MroNI	GCCGGC	1 cut(s)	835
MscI	TGGCCA	1 cut(s)	1226
Msp20I	TGGCCA	1 cut(s)	1226
MspA1I	CMGCKG	3 cut(s)	807, 1045, 1097
MvaI	CCWGG	2 cut(s)	1228, 1240
MvnI	CGCG	9 cut(s)	102, 104, 218, 333, 459, 485, 548, 1097, 1258
MwoI	GCNNNNNNNGC	8 cut(s)	122, 328, 411, 489, 603, 1051, 1094, 1182
NaeI	GCCGGC	1 cut(s)	837
NciI	CCSGG	8 cut(s)	147, 499, 614, 707, 759, 934, 951, 1113
NdeII	GATC	5 cut(s)	230, 693, 915, 991, 1136
NgoMIV	GCCGGC	1 cut(s)	835
NlaIII	CATG	1 cut(s)	1143
NmeAIII	GCCGAG	1 cut(s)	273
NruI	TCGCGA	1 cut(s)	1258
NspV	TTCGAA	1 cut(s)	1157
PaeR7I	CTCGAG	1 cut(s)	476
PaqCI	CACCTGC	1 cut(s)	602
PauI	GCGCGC	1 cut(s)	102
PciSI	GCTCTTC	1 cut(s)	821
PcsI	WCGNNNNNNNCGW	7 cut(s)	510, 552, 561, 564, 852, 1163, 1276
PdiI	GCCGGC	1 cut(s)	837
PfeI	GAWTC	1 cut(s)	128
PkrI	GCNGC	9 cut(s)	118, 121, 124, 461, 652, 732, 1053, 1077, 1099
PleI	GAGTC	2 cut(s)	433, 870
PpsI	GAGTC	2 cut(s)	433, 870
Ppu21I	YACGTR	2 cut(s)	392, 573
PpuMI	RGGWCCY	2 cut(s)	450, 517
Psp5II	RGGWCCY	2 cut(s)	450, 517
Psp6I	CCWGG	2 cut(s)	1226, 1238
PspGI	CCWGG	2 cut(s)	1226, 1238
PspOMI	GGGCCC	3 cut(s)	220, 1091, 1109
PspPPI	RGGWCCY	2 cut(s)	450, 517
PspXI	VCTCGAGB	1 cut(s)	476
PsuI	RGATCY	2 cut(s)	230, 991
PteI	GCGCGC	1 cut(s)	102
PvuII	CAGCTG	1 cut(s)	807
RruI	TCGCGA	1 cut(s)	1258
RsaI	GTAC	3 cut(s)	611, 981, 1282
RsaNI	GTAC	3 cut(s)	610, 980, 1281
SacII	CCGCGG	1 cut(s)	1098
SalI	GTCGAC	1 cut(s)	617
SapI	GCTCTTC	1 cut(s)	821
SatI	GCNGC	9 cut(s)	117, 120, 123, 460, 651, 731, 1052, 1076, 1098
Sau3AI	GATC	5 cut(s)	230, 693, 915, 991, 1136
SchI	GAGTC	2 cut(s)	434, 870
SduI	GDGCHC	7 cut(s)	224, 340, 477, 912, 960, 1095, 1113
SfaNI	GCATC	3 cut(s)	673, 772, 839
Sfr274I	CTCGAG	1 cut(s)	476
Sfr303I	CCGCGG	1 cut(s)	1098
SfuI	TTCGAA	1 cut(s)	1157
SgrBI	CCGCGG	1 cut(s)	1098
SinI	GGWCC	5 cut(s)	371, 380, 450, 517, 947
SlaI	CTCGAG	1 cut(s)	476
SmlI	CTYRAG	3 cut(s)	23, 476, 1214
SmoI	CTYRAG	3 cut(s)	23, 476, 1214
Sse9I	AATT	1 cut(s)	364
SspMI	CTAG	2 cut(s)	341, 1321
TaaI	ACNGT	3 cut(s)	283, 844, 1008
TaiI	ACGT	7 cut(s)	394, 558, 575, 633, 782, 981, 1042
TaqII	GACCGA	1 cut(s)	873
TasI	AATT	1 cut(s)	364
TauI	GCSGC	4 cut(s)	462, 653, 733, 1100
TfiI	GAWTC	1 cut(s)	128
TscAI	CASTG	2 cut(s)	329, 441
TseI	GCWGC	5 cut(s)	116, 119, 122, 1051, 1075
TspDTI	ATGAA	1 cut(s)	839
TspGWI	ACGGA	4 cut(s)	406, 512, 1285, 1289
TspRI	CASTG	2 cut(s)	329, 441
VpaK11BI	GGWCC	5 cut(s)	371, 380, 450, 517, 947
XhoI	CTCGAG	1 cut(s)	476
XmiI	GTMKAC	1 cut(s)	618
XspI	CTAG	2 cut(s)	341, 1321
ZraI	GACGTC	1 cut(s)	631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.