Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002352

Physical Location & Seq

Genomic Coordinates Forward (+)

40452243 .. 40453415

Gene Structure 1173 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G018370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 882 bp

ATGGCCATAATGGTCGGAGTTCTGATGACTCTGTTCTCCTTCATGTTTCTTCTCCTCCTCTACGCCAAGCACTGCAAGCGCGGCAGCTTCGTCGTCATAGGAGGAACCACCGACTCCGGATTTCCCGGCGGGGTGGGTCTCCTTAAAAAATCAGGAATCGACCAGGCCGTCGTCGAGTCCCTCCCGGTTTTCCAGTTCAGGTCTCTCAGGGAACACAAGGACGGCCTCGAATGCACCGTCTGCCTCACCCGAATCGAGCCCACCGAGGTCCTCCGCCTCCTCCAGAAATGCAAGCACCCCTTCCACGTGGAGTGCGTCGACACGTGGCTTGACGCCCACTCGACCTGCCCGCTCTGCCGCTACCGGGTTGACCCGGAAGACATCCTCCTAATCGAAGACGCCAAAATCCTGCACTACCAGAACAGCCAGCCCCAGACTCCCTACCAAAACGACATCGTCATCAACATCGAAACCGACCCAGGAATCCGCAGCGTCTCGGGGCGCCACTCCTCCGCCTTGAGTAGTGACCCGGCTCCTCCTCTCACGACAATACGTCGTCATTCCAGAGGTCCCTCGACAGCTGGATCTGAACGTCCGAGAAAAGACAGCCTCCTTTTAAGCCACGACAAAACGAGGCTGGAGCAAAGAATCATCGTTTCCTCCCCCAGATCAAGCAATACCGGCGGTGGTGGGTTCCACCAACGATGGAGCGACGTACAGCAGTCCGATCTTCTCTACCTTCGCTCCGAGATGATCATAAGCTTGAACGGACTGACACGTCAGAGGCAGGAACAAAGATGTCCTTCTTTGACAGAGAGAGAGAGAGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

293

Amino Acids

33.32

Weight (kDa)

8.13

Isoelectric Point (pI)

62.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	77 - 120	3.2e-12	Ring finger domain
zf-RING_11	PF17123	77 - 106	1e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	77 - 119	1.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	78 - 119	5.8e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	90 - 120	2.4e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05810
fragaria_vesca	FvH4_4g06660
malus_domestica	MD13G1236900.v1.1 MD16G1242200.v1.1
prunus_persica	Prupe.1G069300_v2.0.a1
pyrus_communis	pycom13g20980 pycom16g20300
rosa_chinensis	RchiOBHm_Chr3g0495141 RchiOBHm_Chr4g0398561
rosa_laevigata	RLG00000009344 RLG00000026064 RLG00000029692 RLG00000030599 RLG00000032695 RLG00000032696
rosa_multiflora	Rmu_co8139468.1_g000001 Rmu_co8270033.1_g000001 Rmu_co8275827.1_g000001 Rmu_sc0000185.1_g000029 Rmu_sc0000990.1_g000016 Rmu_sc0005217.1_g000007 Rmu_sc0005996.1_g000027 Rmu_sc0009001.1_g000015 Rmu_sc0013727.1_g000001 Rmu_sc0016350.1_g000001
rosa_roxburghii	Rroxscaffold_5G00343530
rosa_rugosa	Rorug04G0009400
rosa_samantha	Rh1AG207300 Rh2AG231000 Rh2AG449700 Rh2CG436600 Rh2DG238800 Rh3DG271300 Rh4AG087300 Rh4BG083900 Rh4CG095500 Rh4DG078800 Rh4DG124400 Rh5DG085200 Rh6BG230400
rosa_wichuraiana	Rw1G018260 Rw1G018370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	167, 777
Acc36I	ACCTGC	1 cut(s)	353
AccB1I	GGYRCC	1 cut(s)	501
AccBSI	CCGCTC	1 cut(s)	352
AccI	GTMKAC	1 cut(s)	318
AccII	CGCG	1 cut(s)	81
AccIII	TCCGGA	1 cut(s)	116
AciI	CCGC	8 cut(s)	81, 129, 274, 350, 358, 487, 513, 684
AclWI	GGATC	1 cut(s)	592
AcoI	YGGCCR	1 cut(s)	3
AcvI	CACGTG	2 cut(s)	307, 324
AcyI	GRCGYC	3 cut(s)	333, 399, 502
AfaI	GTAC	1 cut(s)	717
AfiI	CCNNNNNNNGG	1 cut(s)	364
AflIII	ACRYGT	2 cut(s)	321, 776
AgsI	TTSAA	1 cut(s)	766
AhdI	GACNNNNNGTC	1 cut(s)	552
AjiI	CACGTC	1 cut(s)	779
AjnI	CCWGG	2 cut(s)	162, 478
AluBI	AGCT	3 cut(s)	87, 581, 762
AluI	AGCT	3 cut(s)	87, 581, 762
Alw26I	GTCTC	3 cut(s)	143, 207, 499
AlwI	GGATC	1 cut(s)	592
Ama87I	CYCGRG	1 cut(s)	496
Aor13HI	TCCGGA	1 cut(s)	116
AoxI	GGCC	3 cut(s)	3, 165, 223
ApeKI	GCWGC	2 cut(s)	84, 489
ArsI	GACNNNNNNTTYG	2 cut(s)	222, 254
AspLEI	GCGC	2 cut(s)	81, 504
AspS9I	GGNCC	2 cut(s)	268, 569
AsuC2I	CCSGG	5 cut(s)	126, 185, 365, 374, 530
AsuHPI	GGTGA	1 cut(s)	238
AvaI	CYCGRG	1 cut(s)	496
AvaII	GGWCC	2 cut(s)	268, 569
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	501
BanII	GRGCYC	1 cut(s)	261
BbrPI	CACGTG	2 cut(s)	307, 324
BbsI	GAAGAC	2 cut(s)	384, 402
BbvI	GCAGC	2 cut(s)	96, 501
BccI	CCATC	1 cut(s)	699
BceAI	ACGGC	2 cut(s)	152, 238
BcgI	CGANNNNNNTGC	2 cut(s)	73, 107
BciT130I	CCWGG	2 cut(s)	164, 480
BclI	TGATCA	1 cut(s)	753
BcnI	CCSGG	5 cut(s)	126, 185, 365, 374, 530
BcoDI	GTCTC	3 cut(s)	143, 207, 499
BfoI	RGCGCY	1 cut(s)	505
BfuAI	ACCTGC	1 cut(s)	353
BisI	GCNGC	4 cut(s)	82, 85, 358, 490
BlsI	GCNGC	4 cut(s)	83, 86, 359, 491
Bme1390I	CCNGG	7 cut(s)	126, 164, 185, 365, 374, 480, 530
Bme18I	GGWCC	2 cut(s)	268, 569
BmeRI	GACNNNNNGTC	1 cut(s)	552
BmeT110I	CYCGRG	1 cut(s)	496
BmgBI	CACGTC	1 cut(s)	779
BmgT120I	GGNCC	2 cut(s)	268, 569
BmiI	GGNNCC	5 cut(s)	106, 503, 534, 571, 695
BmrFI	CCNGG	7 cut(s)	126, 164, 185, 365, 374, 480, 530
BpiI	GAAGAC	2 cut(s)	384, 402
BpmI	CTGGAG	2 cut(s)	266, 659
BpuEI	CTTGAG	1 cut(s)	538
BpuMI	CCSGG	5 cut(s)	126, 185, 365, 374, 530
BsaAI	YACGTR	2 cut(s)	307, 324
BsaHI	GRCGYC	3 cut(s)	333, 399, 502
BsaI	GGTCTC	2 cut(s)	143, 207
BsaJI	CCNNGG	2 cut(s)	264, 478
BsaWI	WCCGGW	1 cut(s)	116
BsaXI	ACNNNNNCTCC	2 cut(s)	728, 758
Bsc4I	CCNNNNNNNGG	1 cut(s)	364
Bse118I	RCCGGY	1 cut(s)	680
Bse1I	ACTGG	1 cut(s)	193
BseAI	TCCGGA	1 cut(s)	116
BseBI	CCWGG	2 cut(s)	164, 480
BseDI	CCNNGG	2 cut(s)	264, 478
BseGI	GGATG	1 cut(s)	381
BseLI	CCNNNNNNNGG	1 cut(s)	364
BseMII	CTCAG	1 cut(s)	220
BseNI	ACTGG	1 cut(s)	193
BseRI	GAGGAG	7 cut(s)	44, 47, 269, 499, 525, 528, 846
BseXI	GCAGC	2 cut(s)	96, 501
BsgI	GTGCAG	1 cut(s)	395
Bsh1236I	CGCG	1 cut(s)	81
BshFI	GGCC	3 cut(s)	5, 167, 225
BshNI	GGYRCC	1 cut(s)	501
BsiHKCI	CYCGRG	1 cut(s)	496
BsiSI	CCGG	7 cut(s)	117, 126, 185, 364, 374, 530, 681
BslFI	GGGAC	2 cut(s)	163, 555
BslI	CCNNNNNNNGG	1 cut(s)	364
BsmAI	GTCTC	3 cut(s)	143, 207, 499
BsmBI	CGTCTC	1 cut(s)	499
BsmFI	GGGAC	2 cut(s)	163, 555
BsmI	GAATGC	1 cut(s)	236
BsnI	GGCC	3 cut(s)	5, 167, 225
Bso31I	GGTCTC	2 cut(s)	143, 207
BsoBI	CYCGRG	1 cut(s)	496
Bsp1286I	GDGCHC	1 cut(s)	261
Bsp13I	TCCGGA	1 cut(s)	116
Bsp143I	GATC	4 cut(s)	584, 668, 727, 753
BspACI	CCGC	8 cut(s)	81, 129, 274, 350, 358, 487, 513, 684
BspANI	GGCC	3 cut(s)	5, 167, 225
BspCNI	CTCAG	1 cut(s)	219
BspEI	TCCGGA	1 cut(s)	116
BspFNI	CGCG	1 cut(s)	81
BspLI	GGNNCC	5 cut(s)	106, 503, 534, 571, 695
BspMI	ACCTGC	1 cut(s)	353
BspPI	GGATC	1 cut(s)	592
BspT107I	GGYRCC	1 cut(s)	501
BspTNI	GGTCTC	2 cut(s)	143, 207
BsrBI	CCGCTC	1 cut(s)	352
BsrFI	RCCGGY	1 cut(s)	680
BsrI	ACTGG	1 cut(s)	193
BssAI	RCCGGY	1 cut(s)	680
BssECI	CCNNGG	2 cut(s)	264, 478
BssMI	GATC	4 cut(s)	584, 668, 727, 753
BssNI	GRCGYC	3 cut(s)	333, 399, 502
Bst2UI	CCWGG	2 cut(s)	164, 480
Bst4CI	ACNGT	1 cut(s)	238
BstACI	GRCGYC	3 cut(s)	333, 399, 502
BstAPI	GCANNNNNTGC	1 cut(s)	240
BstBAI	YACGTR	2 cut(s)	307, 324
BstC8I	GCNNGC	4 cut(s)	77, 293, 350, 428
BstDEI	CTNAG	1 cut(s)	206
BstF5I	GGATG	1 cut(s)	381
BstFNI	CGCG	1 cut(s)	81
BstH2I	RGCGCY	1 cut(s)	505
BstHHI	GCGC	2 cut(s)	81, 504
BstKTI	GATC	4 cut(s)	587, 671, 730, 756
BstMAI	GTCTC	3 cut(s)	143, 207, 499
BstMBI	GATC	4 cut(s)	584, 668, 727, 753
BstMWI	GCNNNNNNNGC	6 cut(s)	76, 81, 231, 240, 354, 681
BstNI	CCWGG	2 cut(s)	164, 480
BstSCI	CCNGG	7 cut(s)	124, 162, 183, 363, 372, 478, 528
BstUI	CGCG	1 cut(s)	81
BstV1I	GCAGC	2 cut(s)	96, 501
BstV2I	GAAGAC	2 cut(s)	384, 402
BstX2I	RGATCY	1 cut(s)	584
BstYI	RGATCY	1 cut(s)	584
BsuRI	GGCC	3 cut(s)	5, 167, 225
BtrI	CACGTC	1 cut(s)	779
BtsCI	GGATG	1 cut(s)	381
BtsI	GCAGTG	1 cut(s)	70
BtsIMutI	CAGTG	1 cut(s)	70
BveI	ACCTGC	1 cut(s)	353
Cac8I	GCNNGC	4 cut(s)	77, 293, 350, 428
CfoI	GCGC	2 cut(s)	81, 504
Cfr10I	RCCGGY	1 cut(s)	680
Cfr13I	GGNCC	2 cut(s)	268, 569
CseI	GACGC	4 cut(s)	304, 341, 407, 481
Csp6I	GTAC	1 cut(s)	716
CviAII	CATG	1 cut(s)	43
CviQI	GTAC	1 cut(s)	716
DdeI	CTNAG	1 cut(s)	206
DinI	GGCGCC	1 cut(s)	503
DpnI	GATC	4 cut(s)	586, 670, 729, 755
DpnII	GATC	4 cut(s)	584, 668, 727, 753
DrdI	GACNNNNNNGTC	2 cut(s)	167, 777
DriI	GACNNNNNGTC	1 cut(s)	552
DseDI	GACNNNNNNGTC	2 cut(s)	167, 777
EaeI	YGGCCR	1 cut(s)	3
Eam1105I	GACNNNNNGTC	1 cut(s)	552
EciI	GGCGGA	2 cut(s)	263, 502
Eco24I	GRGCYC	1 cut(s)	261
Eco31I	GGTCTC	2 cut(s)	143, 207
Eco47I	GGWCC	2 cut(s)	268, 569
Eco72I	CACGTG	2 cut(s)	307, 324
Eco88I	CYCGRG	1 cut(s)	496
EcoO109I	RGGNCCY	2 cut(s)	268, 569
EcoRII	CCWGG	2 cut(s)	162, 478
EcoT38I	GRGCYC	1 cut(s)	261
EgeI	GGCGCC	1 cut(s)	503
EheI	GGCGCC	1 cut(s)	503
Esp3I	CGTCTC	1 cut(s)	499
FaeI	CATG	1 cut(s)	46
FaiI	YATR	4 cut(s)	8, 44, 98, 758
FalI	AAGNNNNNCTT	4 cut(s)	284, 316, 787, 819
FaqI	GGGAC	2 cut(s)	163, 555
FatI	CATG	1 cut(s)	42
FauI	CCCGC	2 cut(s)	122, 357
FbaI	TGATCA	1 cut(s)	753
FblI	GTMKAC	1 cut(s)	318
Fnu4HI	GCNGC	4 cut(s)	82, 85, 358, 490
FokI	GGATG	1 cut(s)	368
FriOI	GRGCYC	1 cut(s)	261
Fsp4HI	GCNGC	4 cut(s)	82, 85, 358, 490
GlaI	GCGC	2 cut(s)	80, 503
GluI	GCNGC	4 cut(s)	82, 85, 358, 490
GsuI	CTGGAG	2 cut(s)	266, 659
HaeII	RGCGCY	1 cut(s)	505
HaeIII	GGCC	3 cut(s)	5, 167, 225
HapII	CCGG	7 cut(s)	117, 126, 185, 364, 374, 530, 681
HgaI	GACGC	4 cut(s)	304, 341, 407, 481
HhaI	GCGC	2 cut(s)	81, 504
Hin1I	GRCGYC	3 cut(s)	333, 399, 502
Hin1II	CATG	1 cut(s)	46
Hin6I	GCGC	2 cut(s)	79, 502
HinP1I	GCGC	2 cut(s)	79, 502
HincII	GTYRAC	2 cut(s)	319, 370
HindII	GTYRAC	2 cut(s)	319, 370
HindIII	AAGCTT	1 cut(s)	760
HinfI	GANTC	8 cut(s)	28, 113, 156, 176, 252, 436, 483, 648
HpaII	CCGG	7 cut(s)	117, 126, 185, 364, 374, 530, 681
HphI	GGTGA	1 cut(s)	238
Hpy166II	GTNNAC	2 cut(s)	319, 370
Hpy188I	TCNGA	7 cut(s)	17, 24, 589, 597, 727, 748, 783
Hpy188III	TCNNGA	5 cut(s)	117, 153, 283, 544, 564
Hpy8I	GTNNAC	2 cut(s)	319, 370
Hpy99I	CGWCG	6 cut(s)	95, 173, 176, 320, 558, 716
HpyAV	CCTTC	4 cut(s)	49, 310, 749, 813
HpyCH4III	ACNGT	1 cut(s)	238
HpyCH4IV	ACGT	6 cut(s)	306, 323, 553, 592, 714, 778
HpyCH4V	TGCA	4 cut(s)	75, 234, 291, 412
HpyF10VI	GCNNNNNNNGC	6 cut(s)	76, 81, 231, 240, 354, 681
HpyF3I	CTNAG	1 cut(s)	206
HpySE526I	ACGT	6 cut(s)	306, 323, 553, 592, 714, 778
Hsp92I	GRCGYC	3 cut(s)	333, 399, 502
Hsp92II	CATG	1 cut(s)	46
HspAI	GCGC	2 cut(s)	79, 502
KasI	GGCGCC	1 cut(s)	501
Kpn2I	TCCGGA	1 cut(s)	116
Ksp22I	TGATCA	1 cut(s)	753
Kzo9I	GATC	4 cut(s)	584, 668, 727, 753
LmnI	GCTCC	4 cut(s)	538, 640, 708, 749
Lsp1109I	GCAGC	2 cut(s)	96, 501
MaeII	ACGT	6 cut(s)	306, 323, 553, 592, 714, 778
MaeIII	GTNAC	1 cut(s)	524
MalI	GATC	4 cut(s)	586, 670, 729, 755
MbiI	CCGCTC	1 cut(s)	352
MboI	GATC	4 cut(s)	584, 668, 727, 753
MboII	GAAGA	4 cut(s)	41, 389, 407, 722
MflI	RGATCY	1 cut(s)	584
MhlI	GDGCHC	1 cut(s)	261
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	502
MlyI	GAGTC	4 cut(s)	22, 107, 185, 430
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	1 cut(s)	116
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	144, 617
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	581
MspI	CCGG	7 cut(s)	117, 126, 185, 364, 374, 530, 681
MspR9I	CCNGG	7 cut(s)	126, 164, 185, 365, 374, 480, 530
Mva1269I	GAATGC	1 cut(s)	236
MvaI	CCWGG	2 cut(s)	164, 480
MvnI	CGCG	1 cut(s)	81
MwoI	GCNNNNNNNGC	6 cut(s)	76, 81, 231, 240, 354, 681
NarI	GGCGCC	1 cut(s)	502
NciI	CCSGG	5 cut(s)	126, 185, 365, 374, 530
NdeII	GATC	4 cut(s)	584, 668, 727, 753
NlaIII	CATG	1 cut(s)	46
NlaIV	GGNNCC	5 cut(s)	106, 503, 534, 571, 695
NmuCI	GTSAC	1 cut(s)	524
PcsI	WCGNNNNNNNCGW	4 cut(s)	165, 234, 261, 312
PctI	GAATGC	1 cut(s)	236
PfeI	GAWTC	4 cut(s)	156, 252, 483, 648
PflFI	GACNNNGTC	1 cut(s)	455
PkrI	GCNGC	4 cut(s)	83, 86, 359, 491
PleI	GAGTC	4 cut(s)	22, 107, 184, 430
PluTI	GGCGCC	1 cut(s)	505
PmaCI	CACGTG	2 cut(s)	307, 324
PmlI	CACGTG	2 cut(s)	307, 324
PpsI	GAGTC	4 cut(s)	22, 107, 184, 430
Ppu21I	YACGTR	2 cut(s)	307, 324
PpuMI	RGGWCCY	2 cut(s)	268, 569
Psp5II	RGGWCCY	2 cut(s)	268, 569
Psp6I	CCWGG	2 cut(s)	162, 478
PspCI	CACGTG	2 cut(s)	307, 324
PspGI	CCWGG	2 cut(s)	162, 478
PspN4I	GGNNCC	5 cut(s)	106, 503, 534, 571, 695
PspPI	GGNCC	2 cut(s)	268, 569
PspPPI	RGGWCCY	2 cut(s)	268, 569
PsuI	RGATCY	1 cut(s)	584
PsyI	GACNNNGTC	1 cut(s)	455
PvuII	CAGCTG	1 cut(s)	581
RsaI	GTAC	1 cut(s)	717
RsaNI	GTAC	1 cut(s)	716
SalI	GTCGAC	1 cut(s)	317
SaqAI	TTAA	2 cut(s)	144, 617
SatI	GCNGC	4 cut(s)	82, 85, 358, 490
Sau3AI	GATC	4 cut(s)	584, 668, 727, 753
Sau96I	GGNCC	2 cut(s)	268, 569
SchI	GAGTC	4 cut(s)	22, 107, 185, 430
ScrFI	CCNGG	7 cut(s)	126, 164, 185, 365, 374, 480, 530
SduI	GDGCHC	1 cut(s)	261
SfoI	GGCGCC	1 cut(s)	503
SinI	GGWCC	2 cut(s)	268, 569
SmlI	CTYRAG	1 cut(s)	517
SmoI	CTYRAG	1 cut(s)	517
SsiI	CCGC	8 cut(s)	81, 129, 274, 350, 358, 487, 513, 684
SspDI	GGCGCC	1 cut(s)	501
StyD4I	CCNGG	7 cut(s)	124, 162, 183, 363, 372, 478, 528
TaaI	ACNGT	1 cut(s)	238
TaiI	ACGT	6 cut(s)	309, 326, 556, 595, 717, 781
TaqI	TCGA	9 cut(s)	159, 174, 228, 255, 318, 341, 393, 468, 575
TauI	GCSGC	2 cut(s)	84, 360
TfiI	GAWTC	4 cut(s)	156, 252, 483, 648
Tru1I	TTAA	2 cut(s)	144, 617
Tru9I	TTAA	2 cut(s)	144, 617
TscAI	CASTG	1 cut(s)	77
TseFI	GTSAC	1 cut(s)	524
TseI	GCWGC	2 cut(s)	84, 489
Tsp45I	GTSAC	1 cut(s)	524
TspDTI	ATGAA	1 cut(s)	31
TspGWI	ACGGA	1 cut(s)	783
TspRI	CASTG	1 cut(s)	77
Tth111I	GACNNNGTC	1 cut(s)	455
VpaK11BI	GGWCC	2 cut(s)	268, 569
XmiI	GTMKAC	1 cut(s)	318

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.