Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002603

Physical Location & Seq

Genomic Coordinates Forward (+)

24497283 .. 24504266

Gene Structure 6984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1239500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGGCCACCGCCCAACCCACCGAGAACGGCTCCGCCAAGGCTGCGAAAGCCGACGCGCAACCACTCACATCCGAAATCACCACCGCCCCGATTACAGCGCCCGAGTCCTACCCAACAACAACAACCGATAACAATCCGAACCAAAATCCCGAACCCGTATCGGACGCCGGGGCTGTCACAACCGCAACGAATGTCGGCAACGATGCCACCTCAGTAGCCGATAAACGATGGCCGGGTTGGCCCGGGGACTGTGTGTTTCGGCTAATTGTGCCGGTTCTGAAGGTCGGTAGCATTATCGGCCGCAAAGGCGAGCTCATTAAGAAGATGTGTGAGGAGACCCGAGCTCGCATTCGAGTCCTCGACGGCGCCGTTGGAAATCCTGATCGCATCGTGCTGATCTCTGGAAGGGAAGAGCCAGAGGCACCTCTTTCCCCTGCAATGGATGCTGTAATAAGAATATTCAAACGTGTCTCTGGATTATCTGAGAGTGAGGGGTCTGGTGCTGCTGGAGTTGCATTCTGTTCCATCCGGTTGTTGGTAGCATCTACCCAAGCAATTAATTTAATTGGAAAACAAGGATCATTAATCAAATCTATACAAGAGAGTACTGGATCGTCTGTGCGAGTATTGTCTGGAGAGGAGGTTCCATTCTATGCTGCCGCAGATGAGAGGATTGTTGAATTGCAGGGAGAAGCTTTAAAGGTTCTCAAAGCTGTAGAAACGGTAGTGGCTCACCTGAGGAAGTTCTTGGTTGATCATAGTGTTATTCCACTTTTTGAGAAGACTTATGCGACAATCTCACAAGAGCGCCAACCCGATACTACCTGGGCTGACAAGTCATTGCTGCATACTGCTTCTCAAACTGGAGTTGGCACTAATTATTCTCTTTCAGCAAAGAGGGAGTCTTTGTTTCTTGATCGTGAAACTCAATTGGAATCACAACTCCCTTCCGCTGGAATTTCACTTTATGGAAAAGATCCTTCAATATATGGAAAAGATCCTCCTATTTATGGACAAGACACTTCTGTTTACGGACAAGGGTCTATTTATGGACAAGATCTTGGTTCTTTTCGTTCTTCAGGAATTGGTCGTCCTGGTGCTCCTATTGTTACTCAGATTGCACAAACAATGCAAATTCCTCTATCTTATGCTGAGGACATTATTGGGGTTGAAGGGTCGAATATTGCATTCATTCGTCGTAGTAGCGGAGCCATTTTAACTGTGCAAGAGAGTAGGGGACTACCTGATGAAATTACCGTAGAAATAAAAGGCACCTCGTCACAGGTTCAGATGGCTCAACAATTGATTCAGGAGGTTATAAACAGTCGGAAGGAACCAGTTACAAGTAACTATGGCGTGATGGATACTGGATTGAGGTCATCCTACTCTCAGTTAGGTGCTCCATCATACCCATCATCAAACGCCTTGTTTGTTCAGCTTCTCTCAAATCTGACAAGAATGCCGACGTTGAATCTGTTTACCAACTTGCCGGTGGACGGAGTTGTGGCCTCTGACATCCTCAAGGACGCCACCAAAGCCGTTTCTAAGATCATTGGCAAACCCGAGTCCGTTAATAATATCTTGTTTCCCATGCAATTCTATATTGTATTGGTGGCTTACAGTATCATTGGGTATTTATGTTTCTGTTTTGGTTTCCCTGTTCTTCAGTATGTGATGATTTTGCTCAACGGGTCTGTGCCGATTGCATTTGCTGGCACGGAAGAGCCTGCTGCGTATGGAGAATTGATCTCCATTGGGGGCATTGGACCAGGTGTTAACGGAAAACTGAGTTCAACCATTGCAGAAATTCTCGAAACTAAGCTCTCCATTGATAGCTCCCGCTTCTATATCAAATTTTATGACGTTGAGGTCCTTCTTCGGGTTCAACGGCTCAACATTTTGATCACAACTGTTCTTCAGCTGATGCTGGCAAATGGCCTTGTATCCCTCAGTTGCAATCTTCTTGTTTTTGATGAAAGAGTAGTCTTCGTGTTTGATTGTATTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022414 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048367 GO:0048438 GO:0048467 GO:0048519 GO:0048577 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048587 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0061458 GO:0065007 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026 GO:2000028 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

670

Amino Acids

71.82

Weight (kDa)

5.16

Isoelectric Point (pI)

39.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	86 - 138	2.9e-12	KH domain
KH_1	PF00013	176 - 242	2.4e-10	KH domain
KH_1	PF00013	375 - 438	8.4e-12	KH domain
MIF	PF01187	556 - 623	3.2e-09	Macrophage migration inhibitory factor (MIF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26000
fragaria_vesca	FvH4_4g07200
malus_domestica	MD13G1239500.v1.1 MD16G1248300.v1.1
prunus_persica	Prupe.1G074800_v2.0.a1
pyrus_communis	pycom16g20810
rosa_chinensis	RchiOBHm_Chr1g0313971 RchiOBHm_Chr2g0148541 RchiOBHm_Chr4g0399461 RchiOBHm_Chr5g0014711 RchiOBHm_Chr6g0261341
rosa_laevigata	RLG00000009266 RLG00000030564 RLG00000030569
rosa_multiflora	Rmu_sc0002255.1_g000002 Rmu_sc0002285.1_g000008 Rmu_sc0039997.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344350
rosa_rugosa	Rorug04G0017100
rosa_samantha	Rh1AG125900 Rh1BG084500 Rh1CG119800 Rh2BG479200 Rh2CG224100 Rh2CG303100 Rh2DG228200 Rh2DG339000 Rh2DG488100 Rh4AG094900 Rh4BG092200 Rh4CG103100 Rh4DG087100 Rh4DG115200 Rh5BG489900 Rh5CG124300 Rh5CG332000 Rh5CG513700 Rh6CG106600
rosa_wichuraiana	Rw1G021120 Rw4G007800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1319
AasI	GACNNNNNNGTC	1 cut(s)	1868
AccB1I	GGYRCC	3 cut(s)	365, 421, 1271
AccII	CGCG	1 cut(s)	56
AciI	CCGC	9 cut(s)	9, 33, 84, 183, 301, 660, 951, 1206, 1840
AclWI	GGATC	4 cut(s)	586, 619, 971, 992
AcoI	YGGCCR	3 cut(s)	3, 230, 298
AcsI	RAATTY	4 cut(s)	957, 1134, 1806, 1853
AcuI	CTGAAG	4 cut(s)	299, 1062, 1649, 1901
AcyI	GRCGYC	3 cut(s)	165, 366, 1527
AfaI	GTAC	1 cut(s)	607
AfiI	CCNNNNNNNGG	6 cut(s)	439, 535, 953, 1010, 1496, 1879
AflIII	ACRYGT	1 cut(s)	466
AgsI	TTSAA	7 cut(s)	463, 680, 984, 1172, 1471, 1794, 1886
AjnI	CCWGG	3 cut(s)	824, 1093, 1768
AloI	GAACNNNNNNTCC	2 cut(s)	627, 659
AluBI	AGCT	8 cut(s)	313, 344, 695, 713, 1438, 1822, 1836, 1921
AluI	AGCT	8 cut(s)	313, 344, 695, 713, 1438, 1822, 1836, 1921
Alw21I	GWGCWC	4 cut(s)	315, 346, 1102, 1402
Alw26I	GTCTC	2 cut(s)	329, 475
AlwI	GGATC	4 cut(s)	586, 619, 971, 992
Ama87I	CYCGRG	4 cut(s)	101, 242, 339, 1562
AoxI	GGCC	6 cut(s)	3, 230, 239, 298, 1506, 1936
ApeKI	GCWGC	5 cut(s)	41, 503, 656, 844, 1730
ApoI	RAATTY	4 cut(s)	957, 1134, 1806, 1853
ArsI	GACNNNNNNTTYG	2 cut(s)	887, 919
AseI	ATTAAT	2 cut(s)	558, 584
AspLEI	GCGC	4 cut(s)	58, 100, 368, 810
AspS9I	GGNCC	3 cut(s)	240, 1766, 1870
AsuC2I	CCSGG	4 cut(s)	169, 234, 243, 244
AsuHPI	GGTGA	2 cut(s)	70, 725
AvaI	CYCGRG	4 cut(s)	101, 242, 339, 1562
AvaII	GGWCC	2 cut(s)	1766, 1870
AxyI	CCTNAGG	1 cut(s)	737
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	365, 421, 1271
BanII	GRGCYC	2 cut(s)	315, 346
BbsI	GAAGAC	2 cut(s)	788, 1978
Bbv12I	GWGCWC	4 cut(s)	315, 346, 1102, 1402
BbvCI	CCTCAGC	1 cut(s)	1152
BbvI	GCAGC	5 cut(s)	28, 490, 643, 831, 1717
BccI	CCATC	6 cut(s)	222, 533, 1285, 1354, 1411, 1420
BceAI	ACGGC	5 cut(s)	43, 353, 379, 1523, 1904
BciT130I	CCWGG	3 cut(s)	826, 1095, 1770
BciVI	GTATCC	2 cut(s)	1357, 1954
BclI	TGATCA	2 cut(s)	754, 1902
BcnI	CCSGG	4 cut(s)	169, 234, 243, 244
BcoDI	GTCTC	2 cut(s)	329, 475
BfmI	CTRYAG	1 cut(s)	714
BfoI	RGCGCY	3 cut(s)	101, 369, 811
BfuI	GTATCC	2 cut(s)	1357, 1954
BglI	GCCNNNNNGGC	2 cut(s)	238, 306
BglII	AGATCT	1 cut(s)	1057
BisI	GCNGC	7 cut(s)	42, 301, 504, 657, 660, 845, 1731
BlsI	GCNGC	7 cut(s)	43, 302, 505, 658, 661, 846, 1732
BmcAI	AGTACT	1 cut(s)	607
Bme1390I	CCNGG	7 cut(s)	169, 234, 243, 244, 826, 1095, 1770
Bme18I	GGWCC	2 cut(s)	1766, 1870
BmeT110I	CYCGRG	4 cut(s)	101, 242, 339, 1562
BmgT120I	GGNCC	3 cut(s)	240, 1766, 1870
BmiI	GGNNCC	7 cut(s)	31, 367, 423, 645, 1210, 1273, 1335
BmrFI	CCNGG	7 cut(s)	169, 234, 243, 244, 826, 1095, 1770
BmsI	GCATC	5 cut(s)	193, 396, 433, 551, 1914
BoxI	GACNNNNGTC	1 cut(s)	1039
BpiI	GAAGAC	2 cut(s)	788, 1978
BplI	GAGNNNNNCTC	2 cut(s)	14, 46
BpmI	CTGGAG	3 cut(s)	528, 654, 885
Bpu10I	CCTNAGC	1 cut(s)	1152
BpuEI	CTTGAG	1 cut(s)	1505
BpuMI	CCSGG	4 cut(s)	169, 234, 243, 244
BsaBI	GATNNNNATC	1 cut(s)	132
BsaHI	GRCGYC	3 cut(s)	165, 366, 1527
BsaI	GGTCTC	1 cut(s)	329
BsaJI	CCNNGG	5 cut(s)	36, 168, 242, 243, 825
BsaWI	WCCGGW	1 cut(s)	528
BsaXI	ACNNNNNCTCC	4 cut(s)	627, 657, 893, 923
Bsc4I	CCNNNNNNNGG	6 cut(s)	439, 535, 953, 1010, 1496, 1879
Bse118I	RCCGGY	2 cut(s)	271, 1489
Bse1I	ACTGG	4 cut(s)	613, 868, 1337, 1372
Bse21I	CCTNAGG	1 cut(s)	737
Bse3DI	GCAATG	3 cut(s)	444, 839, 1797
Bse8I	GATNNNNATC	1 cut(s)	132
BseBI	CCWGG	3 cut(s)	826, 1095, 1770
BseDI	CCNNGG	5 cut(s)	36, 168, 242, 243, 825
BseGI	GGATG	5 cut(s)	68, 448, 525, 1379, 1515
BseJI	GATNNNNATC	1 cut(s)	132
BseLI	CCNNNNNNNGG	6 cut(s)	439, 535, 953, 1010, 1496, 1879
BseMI	GCAATG	3 cut(s)	444, 839, 1797
BseMII	CTCAG	8 cut(s)	225, 474, 728, 1127, 1143, 1403, 1778, 1963
BseNI	ACTGG	4 cut(s)	613, 868, 1337, 1372
BseRI	GAGGAG	2 cut(s)	347, 653
BseX3I	CGGCCG	1 cut(s)	298
BseXI	GCAGC	5 cut(s)	28, 490, 643, 831, 1717
Bsh1236I	CGCG	1 cut(s)	56
Bsh1285I	CGRYCG	1 cut(s)	301
BshFI	GGCC	6 cut(s)	5, 232, 241, 300, 1508, 1938
BshNI	GGYRCC	3 cut(s)	365, 421, 1271
BsiEI	CGRYCG	1 cut(s)	301
BsiHKAI	GWGCWC	4 cut(s)	315, 346, 1102, 1402
BsiHKCI	CYCGRG	4 cut(s)	101, 242, 339, 1562
BsiSI	CCGG	6 cut(s)	168, 233, 243, 272, 529, 1490
BslFI	GGGAC	2 cut(s)	260, 1251
BslI	CCNNNNNNNGG	6 cut(s)	439, 535, 953, 1010, 1496, 1879
BsmAI	GTCTC	2 cut(s)	329, 475
BsmFI	GGGAC	2 cut(s)	260, 1251
BsmI	GAATGC	4 cut(s)	348, 515, 1187, 1464
BsnI	GGCC	6 cut(s)	5, 232, 241, 300, 1508, 1938
Bso31I	GGTCTC	1 cut(s)	329
BsoBI	CYCGRG	4 cut(s)	101, 242, 339, 1562
Bsp1286I	GDGCHC	4 cut(s)	315, 346, 1102, 1402
BspACI	CCGC	9 cut(s)	9, 33, 84, 183, 301, 660, 951, 1206, 1840
BspANI	GGCC	6 cut(s)	5, 232, 241, 300, 1508, 1938
BspCNI	CTCAG	8 cut(s)	224, 475, 729, 1126, 1144, 1402, 1779, 1962
BspFNI	CGCG	1 cut(s)	56
BspLI	GGNNCC	7 cut(s)	31, 367, 423, 645, 1210, 1273, 1335
BspPI	GGATC	4 cut(s)	586, 619, 971, 992
BspQI	GCTCTTC	2 cut(s)	405, 1716
BspT107I	GGYRCC	3 cut(s)	365, 421, 1271
BspTNI	GGTCTC	1 cut(s)	329
BsrDI	GCAATG	3 cut(s)	444, 839, 1797
BsrFI	RCCGGY	2 cut(s)	271, 1489
BsrI	ACTGG	4 cut(s)	613, 868, 1337, 1372
BssAI	RCCGGY	2 cut(s)	271, 1489
BssECI	CCNNGG	5 cut(s)	36, 168, 242, 243, 825
BssNI	GRCGYC	3 cut(s)	165, 366, 1527
BssT1I	CCWWGG	1 cut(s)	36
Bst2UI	CCWGG	3 cut(s)	826, 1095, 1770
Bst4CI	ACNGT	7 cut(s)	251, 724, 1222, 1258, 1325, 1622, 1912
Bst6I	CTCTTC	2 cut(s)	405, 1716
BstACI	GRCGYC	3 cut(s)	165, 366, 1527
BstAPI	GCANNNNNTGC	1 cut(s)	443
BstC8I	GCNNGC	5 cut(s)	311, 346, 1714, 1728, 1929
BstF5I	GGATG	5 cut(s)	68, 448, 525, 1379, 1515
BstFNI	CGCG	1 cut(s)	56
BstH2I	RGCGCY	3 cut(s)	101, 369, 811
BstHHI	GCGC	4 cut(s)	58, 100, 368, 810
BstMAI	GTCTC	2 cut(s)	329, 475
BstMCI	CGRYCG	1 cut(s)	301
BstMWI	GCNNNNNNNGC	9 cut(s)	41, 47, 238, 268, 297, 306, 443, 512, 1535
BstNI	CCWGG	3 cut(s)	826, 1095, 1770
BstPAI	GACNNNNGTC	1 cut(s)	1039
BstSCI	CCNGG	7 cut(s)	167, 232, 241, 242, 824, 1093, 1768
BstSFI	CTRYAG	1 cut(s)	714
BstUI	CGCG	1 cut(s)	56
BstV1I	GCAGC	5 cut(s)	28, 490, 643, 831, 1717
BstV2I	GAAGAC	2 cut(s)	788, 1978
BstX2I	RGATCY	3 cut(s)	976, 997, 1057
BstYI	RGATCY	3 cut(s)	976, 997, 1057
BstZI	CGGCCG	1 cut(s)	298
Bsu36I	CCTNAGG	1 cut(s)	737
BsuI	GTATCC	2 cut(s)	1357, 1954
BsuRI	GGCC	6 cut(s)	5, 232, 241, 300, 1508, 1938
BtsCI	GGATG	5 cut(s)	68, 448, 525, 1379, 1515
Cac8I	GCNNGC	5 cut(s)	311, 346, 1714, 1728, 1929
CfoI	GCGC	4 cut(s)	58, 100, 368, 810
Cfr10I	RCCGGY	2 cut(s)	271, 1489
Cfr13I	GGNCC	3 cut(s)	240, 1766, 1870
Cfr9I	CCCGGG	1 cut(s)	242
CseI	GACGC	3 cut(s)	62, 173, 1535
CsiI	ACCWGGT	1 cut(s)	1768
Csp6I	GTAC	1 cut(s)	606
CviAII	CATG	1 cut(s)	1591
CviQI	GTAC	1 cut(s)	606
DinI	GGCGCC	1 cut(s)	367
DraI	TTTAAA	1 cut(s)	699
DrdI	GACNNNNNNGTC	1 cut(s)	1868
DseDI	GACNNNNNNGTC	1 cut(s)	1868
EaeI	YGGCCR	3 cut(s)	3, 230, 298
EagI	CGGCCG	1 cut(s)	298
Eam1104I	CTCTTC	2 cut(s)	405, 1716
EarI	CTCTTC	2 cut(s)	405, 1716
EciI	GGCGGA	1 cut(s)	22
Ecl136II	GAGCTC	2 cut(s)	313, 344
EclXI	CGGCCG	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	36
Eco24I	GRGCYC	2 cut(s)	315, 346
Eco31I	GGTCTC	1 cut(s)	329
Eco47I	GGWCC	2 cut(s)	1766, 1870
Eco52I	CGGCCG	1 cut(s)	298
Eco53kI	GAGCTC	2 cut(s)	313, 344
Eco57I	CTGAAG	4 cut(s)	299, 1062, 1649, 1901
Eco81I	CCTNAGG	1 cut(s)	737
Eco88I	CYCGRG	4 cut(s)	101, 242, 339, 1562
EcoICRI	GAGCTC	2 cut(s)	313, 344
EcoO109I	RGGNCCY	1 cut(s)	1870
EcoRII	CCWGG	3 cut(s)	824, 1093, 1768
EcoT14I	CCWWGG	1 cut(s)	36
EcoT38I	GRGCYC	2 cut(s)	315, 346
EgeI	GGCGCC	1 cut(s)	367
EheI	GGCGCC	1 cut(s)	367
ErhI	CCWWGG	1 cut(s)	36
FaeI	CATG	1 cut(s)	1594
FaqI	GGGAC	2 cut(s)	260, 1251
FatI	CATG	1 cut(s)	1590
FauI	CCCGC	1 cut(s)	1847
FbaI	TGATCA	2 cut(s)	754, 1902
Fnu4HI	GCNGC	7 cut(s)	42, 301, 504, 657, 660, 845, 1731
FokI	GGATG	5 cut(s)	55, 455, 512, 1366, 1502
FriOI	GRGCYC	2 cut(s)	315, 346
Fsp4HI	GCNGC	7 cut(s)	42, 301, 504, 657, 660, 845, 1731
GlaI	GCGC	4 cut(s)	57, 99, 367, 809
GluI	GCNGC	7 cut(s)	42, 301, 504, 657, 660, 845, 1731
GsuI	CTGGAG	3 cut(s)	528, 654, 885
HaeII	RGCGCY	3 cut(s)	101, 369, 811
HaeIII	GGCC	6 cut(s)	5, 232, 241, 300, 1508, 1938
HapII	CCGG	6 cut(s)	168, 233, 243, 272, 529, 1490
HgaI	GACGC	3 cut(s)	62, 173, 1535
HhaI	GCGC	4 cut(s)	58, 100, 368, 810
Hin1I	GRCGYC	3 cut(s)	165, 366, 1527
Hin1II	CATG	1 cut(s)	1594
Hin6I	GCGC	4 cut(s)	56, 98, 366, 808
HinP1I	GCGC	4 cut(s)	56, 98, 366, 808
HincII	GTYRAC	1 cut(s)	1777
HindII	GTYRAC	1 cut(s)	1777
HindIII	AAGCTT	1 cut(s)	693
HinfI	GANTC	7 cut(s)	104, 354, 902, 935, 1306, 1471, 1565
HpaI	GTTAAC	1 cut(s)	1777
HpaII	CCGG	6 cut(s)	168, 233, 243, 272, 529, 1490
HphI	GGTGA	2 cut(s)	70, 725
Hpy166II	GTNNAC	4 cut(s)	1030, 1479, 1495, 1777
Hpy8I	GTNNAC	4 cut(s)	1030, 1479, 1495, 1777
Hpy99I	CGWCG	4 cut(s)	56, 365, 1200, 1468
HpyAV	CCTTC	7 cut(s)	274, 399, 957, 990, 1166, 1324, 1883
HpyCH4III	ACNGT	7 cut(s)	251, 724, 1222, 1258, 1325, 1622, 1912
HpyCH4IV	ACGT	3 cut(s)	466, 1466, 1863
HpyF10VI	GCNNNNNNNGC	9 cut(s)	41, 47, 238, 268, 297, 306, 443, 512, 1535
HpySE526I	ACGT	3 cut(s)	466, 1466, 1863
Hsp92I	GRCGYC	3 cut(s)	165, 366, 1527
Hsp92II	CATG	1 cut(s)	1594
HspAI	GCGC	4 cut(s)	56, 98, 366, 808
KasI	GGCGCC	1 cut(s)	365
Ksp22I	TGATCA	2 cut(s)	754, 1902
KspAI	GTTAAC	1 cut(s)	1777
LguI	GCTCTTC	2 cut(s)	405, 1716
LmnI	GCTCC	5 cut(s)	35, 1105, 1208, 1405, 1841
Lsp1109I	GCAGC	5 cut(s)	28, 490, 643, 831, 1717
LweI	GCATC	5 cut(s)	193, 396, 433, 551, 1914
MabI	ACCWGGT	1 cut(s)	1768
MaeII	ACGT	3 cut(s)	466, 1466, 1863
MaeIII	GTNAC	5 cut(s)	175, 1108, 1278, 1339, 1346
MfeI	CAATTG	2 cut(s)	929, 1301
MflI	RGATCY	3 cut(s)	976, 997, 1057
MhlI	GDGCHC	4 cut(s)	315, 346, 1102, 1402
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	366
MlyI	GAGTC	4 cut(s)	113, 363, 911, 1574
MmeI	TCCRAC	2 cut(s)	352, 1307
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	8 cut(s)	318, 558, 563, 584, 698, 1217, 1572, 1776
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	953, 1921
MspI	CCGG	6 cut(s)	168, 233, 243, 272, 529, 1490
MspR9I	CCNGG	7 cut(s)	169, 234, 243, 244, 826, 1095, 1770
MunI	CAATTG	2 cut(s)	929, 1301
Mva1269I	GAATGC	4 cut(s)	348, 515, 1187, 1464
MvaI	CCWGG	3 cut(s)	826, 1095, 1770
MvnI	CGCG	1 cut(s)	56
MwoI	GCNNNNNNNGC	9 cut(s)	41, 47, 238, 268, 297, 306, 443, 512, 1535
NarI	GGCGCC	1 cut(s)	366
NciI	CCSGG	4 cut(s)	169, 234, 243, 244
NlaIII	CATG	1 cut(s)	1594
NlaIV	GGNNCC	7 cut(s)	31, 367, 423, 645, 1210, 1273, 1335
NmuCI	GTSAC	2 cut(s)	175, 1278
PciSI	GCTCTTC	2 cut(s)	405, 1716
PcsI	WCGNNNNNNNCGW	1 cut(s)	366
PctI	GAATGC	4 cut(s)	348, 515, 1187, 1464
PfeI	GAWTC	3 cut(s)	935, 1306, 1471
PkrI	GCNGC	7 cut(s)	43, 302, 505, 658, 661, 846, 1732
PleI	GAGTC	4 cut(s)	112, 362, 910, 1573
PluTI	GGCGCC	1 cut(s)	369
PpsI	GAGTC	4 cut(s)	112, 362, 910, 1573
PpuMI	RGGWCCY	1 cut(s)	1870
PshAI	GACNNNNGTC	1 cut(s)	1039
PshBI	ATTAAT	2 cut(s)	558, 584
PsiI	TTATAA	1 cut(s)	1319
Psp124BI	GAGCTC	2 cut(s)	315, 346
Psp5II	RGGWCCY	1 cut(s)	1870
Psp6I	CCWGG	3 cut(s)	824, 1093, 1768
PspGI	CCWGG	3 cut(s)	824, 1093, 1768
PspN4I	GGNNCC	7 cut(s)	31, 367, 423, 645, 1210, 1273, 1335
PspPI	GGNCC	3 cut(s)	240, 1766, 1870
PspPPI	RGGWCCY	1 cut(s)	1870
PsuI	RGATCY	3 cut(s)	976, 997, 1057
PvuII	CAGCTG	1 cut(s)	1921
RsaI	GTAC	1 cut(s)	607
RsaNI	GTAC	1 cut(s)	606
SacI	GAGCTC	2 cut(s)	315, 346
SapI	GCTCTTC	2 cut(s)	405, 1716
SaqAI	TTAA	8 cut(s)	318, 558, 563, 584, 698, 1217, 1572, 1776
SatI	GCNGC	7 cut(s)	42, 301, 504, 657, 660, 845, 1731
Sau96I	GGNCC	3 cut(s)	240, 1766, 1870
ScaI	AGTACT	1 cut(s)	607
SchI	GAGTC	4 cut(s)	113, 363, 911, 1574
ScrFI	CCNGG	7 cut(s)	169, 234, 243, 244, 826, 1095, 1770
SduI	GDGCHC	4 cut(s)	315, 346, 1102, 1402
SexAI	ACCWGGT	1 cut(s)	1768
SfaNI	GCATC	5 cut(s)	193, 396, 433, 551, 1914
SfcI	CTRYAG	1 cut(s)	714
SfiI	GGCCNNNNNGGCC	1 cut(s)	238
SfoI	GGCGCC	1 cut(s)	367
SinI	GGWCC	2 cut(s)	1766, 1870
SmaI	CCCGGG	1 cut(s)	244
SmlI	CTYRAG	1 cut(s)	1520
SmoI	CTYRAG	1 cut(s)	1520
SsiI	CCGC	9 cut(s)	9, 33, 84, 183, 301, 660, 951, 1206, 1840
SspDI	GGCGCC	1 cut(s)	365
SspI	AATATT	2 cut(s)	459, 1183
SstI	GAGCTC	2 cut(s)	315, 346
StyD4I	CCNGG	7 cut(s)	167, 232, 241, 242, 824, 1093, 1768
StyI	CCWWGG	1 cut(s)	36
TaaI	ACNGT	7 cut(s)	251, 724, 1222, 1258, 1325, 1622, 1912
TaiI	ACGT	3 cut(s)	469, 1469, 1866
TaqI	TCGA	4 cut(s)	352, 360, 1178, 1812
TatI	WGTACW	1 cut(s)	605
TauI	GCSGC	2 cut(s)	303, 662
TfiI	GAWTC	3 cut(s)	935, 1306, 1471
Tru1I	TTAA	8 cut(s)	318, 558, 563, 584, 698, 1217, 1572, 1776
Tru9I	TTAA	8 cut(s)	318, 558, 563, 584, 698, 1217, 1572, 1776
TseFI	GTSAC	2 cut(s)	175, 1278
TseI	GCWGC	5 cut(s)	41, 503, 656, 844, 1730
Tsp45I	GTSAC	2 cut(s)	175, 1278
TspDTI	ATGAA	3 cut(s)	1180, 1263, 1989
TspGWI	ACGGA	5 cut(s)	1047, 1512, 1558, 1733, 1794
TspMI	CCCGGG	1 cut(s)	242
VpaK11BI	GGWCC	2 cut(s)	1766, 1870
VspI	ATTAAT	2 cut(s)	558, 584
XapI	RAATTY	4 cut(s)	957, 1134, 1806, 1853
XcmI	CCANNNNNNNNNTGG	2 cut(s)	532, 1489
XmaI	CCCGGG	1 cut(s)	242
ZrmI	AGTACT	1 cut(s)	607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.