Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002603

Physical Location & Seq

Genomic Coordinates Reverse (-)

27718970 .. 27723824

Gene Structure 4855 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1248300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGACCACCGCACAACCCACCAAGAACGGCTTCGTCAACGCTGCGGAAGCTGACGCCCAGCCACTCACATCCGAAACCACCCCCGCCCCAATTACAGTGCCCGAGTCCGACCCAACAACAGATAACAATCCGGACCAAAATCCCGAACCCGAATCTGGTCCCAATTCGGACACCGGGGCTGCCACTACCCCAACGAACGTCGGCAACGATTCCACCTCCATAGCTGATAATCGATGGCCGGGTTGGCCCGGGGACTGCGTGTTTCGGCTAATTGTGCCGGTTCTGAAGGTCGGTAGCATTATTGGCCGCAAAGGCGAGCTCATTAAGAAGATGTGCGAGGAGACCCGAGCTCGCATTCGGGTCCTGGACGGCGCTGTTGGAAATCCTGATCGCATCGTGCTGATCTCTGCAAGGGAAGAGCCAGAGGTACCTCTGTCACCTGCAATGGATGCTGTAATAAGAATATTCAAACGTGTCTCTGGATTAACTGAGAGTGAGGGGTCTGGTGCTGCTGGAGTTGCATTCTGTTCCATCCGGTTGTTGGTAGCATCTACCCAAGCAATTAATTTAATTGGAAAACAAGGATCATTAATCAAATCTATACAAGAGAGTACCGGATCTTCTGTGCGAGTATTGTCCGGAGAGGAGGTTCCGTCCTATGCTGCTGCAGATGAGAGGATTGTTGAATTGCAGGGAGAAGCTTTAAAGGTTCTCAAAGCTGTAGAAGCGGTAGTGGCTCACCTGAGGAAGTTTTTGGTTGATCATAGTGTTATTCCGCTTTTTGAGAAGACACATTCGACAATCTCACAAGAGCGCCAACCAGATACTACCTGGGCTGACAAGTCGTTGCTGCATACTGCTTCTCAAACTGGAGTGAGCACTAATTACCCTCTCTCATCAAAGAGGGAGTCTTTGTTTCTTGAACGTGAAACTCAATTGGAATCACAACTCCCTTCTGCTGGAATTTCACTTTATGGAAAAGATCTTTCTATTTATGGAAAATATCCTTCTATTTATGGACAAGACCCTTCTGTTTACAGGCAAGGGTCTATTTATGGACAAGATCCTTCTCTTTCTTCTATCCGTTCTTCGGGAATTGGTCGTCCCGGTGCTCCTATTGTTACTCAGATTGCACAAACAATGCAAATTCCTCTATCTTATGCGGAGGACATTATCGGGGTTGAAGGGTCAAATATTGCATTCATTCGTCGCAGTAGCGGAGCCATCTTAACTGTGCAAGAGAGTAGGGGACTACCTGATGAAATTACTGTAGAAATAAAAGGCACCTCATCACAGGTTCAGATGGCTCAACAACTGATTCAGGAGGTTATAAACAGTCGCAAGGAACCAGTTACAAGTAACTATGGCGTGATGGATACTGGATTGAGGTCATCCTACTCTCAGTTAGGTGCTCCATCCTACCCATCATCGTCACTACCCTCACAACCCTACGGCGGCAATTATGGATCGTCTGGTCCAGGAGGAGGCTACAGCATGTTTAGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022414 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048367 GO:0048438 GO:0048467 GO:0048519 GO:0048577 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048587 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0061458 GO:0065007 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:2000026 GO:2000028 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

503

Amino Acids

53.53

Weight (kDa)

5.19

Isoelectric Point (pI)

39.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	88 - 139	2.4e-11	KH domain
KH_1	PF00013	178 - 244	1e-10	KH domain
KH_1	PF00013	379 - 442	5.6e-12	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26000
fragaria_vesca	FvH4_4g07200
malus_domestica	MD13G1239500.v1.1 MD16G1248300.v1.1
prunus_persica	Prupe.1G074800_v2.0.a1
pyrus_communis	pycom16g20810
rosa_chinensis	RchiOBHm_Chr1g0313971 RchiOBHm_Chr2g0148541 RchiOBHm_Chr4g0399461 RchiOBHm_Chr5g0014711 RchiOBHm_Chr6g0261341
rosa_laevigata	RLG00000009266 RLG00000030564 RLG00000030569
rosa_multiflora	Rmu_sc0002255.1_g000002 Rmu_sc0002285.1_g000008 Rmu_sc0039997.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344350
rosa_rugosa	Rorug04G0017100
rosa_samantha	Rh1AG125900 Rh1BG084500 Rh1CG119800 Rh2BG479200 Rh2CG224100 Rh2CG303100 Rh2DG228200 Rh2DG339000 Rh2DG488100 Rh4AG094900 Rh4BG092200 Rh4CG103100 Rh4DG087100 Rh4DG115200 Rh5BG489900 Rh5CG124300 Rh5CG332000 Rh5CG513700 Rh6CG106600
rosa_wichuraiana	Rw1G021120 Rw4G007800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1331
AarI	CACCTGC	1 cut(s)	448
Acc36I	ACCTGC	1 cut(s)	448
Acc65I	GGTACC	1 cut(s)	427
AccB1I	GGYRCC	2 cut(s)	427, 1283
AccIII	TCCGGA	2 cut(s)	130, 638
AciI	CCGC	9 cut(s)	9, 44, 84, 307, 728, 776, 1163, 1218, 1455
AclWI	GGATC	4 cut(s)	592, 625, 1058, 1474
AcoI	YGGCCR	2 cut(s)	236, 304
AcsI	RAATTY	2 cut(s)	963, 1146
AcuI	CTGAAG	1 cut(s)	305
AcyI	GRCGYC	1 cut(s)	54
AfaI	GTAC	2 cut(s)	429, 613
AfiI	CCNNNNNNNGG	6 cut(s)	155, 541, 959, 1090, 1454, 1484
AflIII	ACRYGT	1 cut(s)	472
AgsI	TTSAA	4 cut(s)	469, 686, 923, 1184
AjnI	CCWGG	3 cut(s)	363, 830, 1477
AjuI	GAANNNNNNNTTGG	2 cut(s)	14, 46
AloI	GAACNNNNNNTCC	2 cut(s)	633, 665
AluBI	AGCT	6 cut(s)	50, 224, 319, 350, 701, 719
AluI	AGCT	6 cut(s)	50, 224, 319, 350, 701, 719
Alw21I	GWGCWC	5 cut(s)	321, 352, 881, 1114, 1414
Alw26I	GTCTC	2 cut(s)	335, 481
AlwI	GGATC	4 cut(s)	592, 625, 1058, 1474
Ama87I	CYCGRG	3 cut(s)	101, 248, 345
Aor13HI	TCCGGA	2 cut(s)	130, 638
AoxI	GGCC	3 cut(s)	236, 245, 304
ApeKI	GCWGC	6 cut(s)	41, 179, 509, 662, 665, 850
ApoI	RAATTY	2 cut(s)	963, 1146
ArsI	GACNNNNNNTTYG	2 cut(s)	893, 925
AseI	ATTAAT	2 cut(s)	564, 590
Asp700I	GAANNNNTTC	1 cut(s)	29
Asp718I	GGTACC	1 cut(s)	427
AspLEI	GCGC	2 cut(s)	374, 816
AspS9I	GGNCC	5 cut(s)	133, 158, 246, 361, 1475
AsuC2I	CCSGG	5 cut(s)	175, 240, 249, 250, 1107
AsuHPI	GGTGA	2 cut(s)	429, 731
AvaI	CYCGRG	3 cut(s)	101, 248, 345
AvaII	GGWCC	4 cut(s)	133, 158, 361, 1475
AxyI	CCTNAGG	1 cut(s)	743
BaeGI	GKGCMC	1 cut(s)	102
BaeI	ACNNNNGTAYC	2 cut(s)	419, 452
BanI	GGYRCC	2 cut(s)	427, 1283
BanII	GRGCYC	2 cut(s)	321, 352
BarI	GAAGNNNNNNTAC	2 cut(s)	604, 636
BbsI	GAAGAC	1 cut(s)	794
Bbv12I	GWGCWC	5 cut(s)	321, 352, 881, 1114, 1414
BbvI	GCAGC	6 cut(s)	28, 166, 496, 649, 652, 837
BccI	CCATC	7 cut(s)	228, 539, 1232, 1297, 1366, 1423, 1432
BceAI	ACGGC	3 cut(s)	43, 385, 1468
BciT130I	CCWGG	3 cut(s)	365, 832, 1479
BciVI	GTATCC	1 cut(s)	1369
BclI	TGATCA	1 cut(s)	760
BcnI	CCSGG	5 cut(s)	175, 240, 249, 250, 1107
BcoDI	GTCTC	2 cut(s)	335, 481
BfmI	CTRYAG	4 cut(s)	666, 720, 1269, 1489
BfoI	RGCGCY	2 cut(s)	375, 817
BfuAI	ACCTGC	1 cut(s)	448
BfuI	GTATCC	1 cut(s)	1369
BglI	GCCNNNNNGGC	2 cut(s)	244, 312
BglII	AGATCT	1 cut(s)	982
BisI	GCNGC	8 cut(s)	42, 180, 307, 510, 663, 666, 851, 1456
BlsI	GCNGC	8 cut(s)	43, 181, 308, 511, 664, 667, 852, 1457
Bme1390I	CCNGG	8 cut(s)	175, 240, 249, 250, 365, 832, 1107, 1479
Bme18I	GGWCC	4 cut(s)	133, 158, 361, 1475
BmeT110I	CYCGRG	3 cut(s)	101, 248, 345
BmgT120I	GGNCC	5 cut(s)	133, 158, 246, 361, 1475
BmiI	GGNNCC	7 cut(s)	160, 362, 429, 651, 1222, 1285, 1347
BmrFI	CCNGG	8 cut(s)	175, 240, 249, 250, 365, 832, 1107, 1479
BmsI	GCATC	3 cut(s)	402, 439, 557
BpiI	GAAGAC	1 cut(s)	794
BpmI	CTGGAG	2 cut(s)	534, 891
BpuMI	CCSGG	5 cut(s)	175, 240, 249, 250, 1107
Bsa29I	ATCGAT	1 cut(s)	232
BsaBI	GATNNNNATC	1 cut(s)	126
BsaHI	GRCGYC	1 cut(s)	54
BsaI	GGTCTC	1 cut(s)	335
BsaJI	CCNNGG	4 cut(s)	174, 248, 249, 831
BsaWI	WCCGGW	4 cut(s)	130, 534, 614, 638
BsaXI	ACNNNNNCTCC	6 cut(s)	633, 638, 663, 668, 899, 929
Bsc4I	CCNNNNNNNGG	6 cut(s)	155, 541, 959, 1090, 1454, 1484
Bse118I	RCCGGY	1 cut(s)	277
Bse1I	ACTGG	3 cut(s)	874, 1349, 1384
Bse21I	CCTNAGG	1 cut(s)	743
Bse3DI	GCAATG	1 cut(s)	450
Bse8I	GATNNNNATC	1 cut(s)	126
BseAI	TCCGGA	2 cut(s)	130, 638
BseBI	CCWGG	3 cut(s)	365, 832, 1479
BseCI	ATCGAT	1 cut(s)	232
BseDI	CCNNGG	4 cut(s)	174, 248, 249, 831
BseGI	GGATG	5 cut(s)	68, 454, 531, 1391, 1415
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	6 cut(s)	155, 541, 959, 1090, 1454, 1484
BseMI	GCAATG	1 cut(s)	450
BseMII	CTCAG	4 cut(s)	480, 734, 1139, 1415
BseNI	ACTGG	3 cut(s)	874, 1349, 1384
BseRI	GAGGAG	3 cut(s)	353, 659, 1497
BseSI	GKGCMC	1 cut(s)	102
BseXI	GCAGC	6 cut(s)	28, 166, 496, 649, 652, 837
BseYI	CCCAGC	1 cut(s)	57
BshFI	GGCC	3 cut(s)	238, 247, 306
BshNI	GGYRCC	2 cut(s)	427, 1283
BshVI	ATCGAT	1 cut(s)	232
BsiHKAI	GWGCWC	5 cut(s)	321, 352, 881, 1114, 1414
BsiHKCI	CYCGRG	3 cut(s)	101, 248, 345
BsiSI	CCGG	9 cut(s)	131, 174, 239, 249, 278, 535, 615, 639, 1107
BslFI	GGGAC	4 cut(s)	144, 266, 1089, 1263
BslI	CCNNNNNNNGG	6 cut(s)	155, 541, 959, 1090, 1454, 1484
BsmAI	GTCTC	2 cut(s)	335, 481
BsmFI	GGGAC	4 cut(s)	144, 266, 1089, 1263
BsmI	GAATGC	3 cut(s)	354, 521, 1199
BsnI	GGCC	3 cut(s)	238, 247, 306
Bso31I	GGTCTC	1 cut(s)	335
BsoBI	CYCGRG	3 cut(s)	101, 248, 345
Bsp1286I	GDGCHC	6 cut(s)	102, 321, 352, 881, 1114, 1414
Bsp13I	TCCGGA	2 cut(s)	130, 638
Bsp143I	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
BspACI	CCGC	9 cut(s)	9, 44, 84, 307, 728, 776, 1163, 1218, 1455
BspANI	GGCC	3 cut(s)	238, 247, 306
BspCNI	CTCAG	4 cut(s)	481, 735, 1138, 1414
BspDI	ATCGAT	1 cut(s)	232
BspEI	TCCGGA	2 cut(s)	130, 638
BspLI	GGNNCC	7 cut(s)	160, 362, 429, 651, 1222, 1285, 1347
BspMAI	CTGCAG	1 cut(s)	670
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	4 cut(s)	592, 625, 1058, 1474
BspQI	GCTCTTC	1 cut(s)	411
BspT107I	GGYRCC	2 cut(s)	427, 1283
BspTNI	GGTCTC	1 cut(s)	335
BsrDI	GCAATG	1 cut(s)	450
BsrFI	RCCGGY	1 cut(s)	277
BsrI	ACTGG	3 cut(s)	874, 1349, 1384
BssAI	RCCGGY	1 cut(s)	277
BssECI	CCNNGG	4 cut(s)	174, 248, 249, 831
BssMI	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
BssNI	GRCGYC	1 cut(s)	54
Bst2UI	CCWGG	3 cut(s)	365, 832, 1479
Bst4CI	ACNGT	4 cut(s)	97, 1234, 1270, 1337
Bst6I	CTCTTC	1 cut(s)	411
BstACI	GRCGYC	1 cut(s)	54
BstAPI	GCANNNNNTGC	1 cut(s)	449
BstC8I	GCNNGC	2 cut(s)	317, 352
BstDEI	CTNAG	4 cut(s)	489, 743, 1125, 1401
BstF5I	GGATG	5 cut(s)	68, 454, 531, 1391, 1415
BstH2I	RGCGCY	2 cut(s)	375, 817
BstHHI	GCGC	2 cut(s)	374, 816
BstKTI	GATC	8 cut(s)	391, 405, 587, 620, 763, 985, 1066, 1469
BstMAI	GTCTC	2 cut(s)	335, 481
BstMBI	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
BstMWI	GCNNNNNNNGC	9 cut(s)	47, 244, 274, 303, 312, 449, 518, 725, 734
BstNI	CCWGG	3 cut(s)	365, 832, 1479
BstNSI	RCATGY	1 cut(s)	1498
BstSCI	CCNGG	8 cut(s)	173, 238, 247, 248, 363, 830, 1105, 1477
BstSFI	CTRYAG	4 cut(s)	666, 720, 1269, 1489
BstSLI	GKGCMC	1 cut(s)	102
BstV1I	GCAGC	6 cut(s)	28, 166, 496, 649, 652, 837
BstV2I	GAAGAC	1 cut(s)	794
BstX2I	RGATCY	3 cut(s)	617, 982, 1063
BstYI	RGATCY	3 cut(s)	617, 982, 1063
Bsu15I	ATCGAT	1 cut(s)	232
Bsu36I	CCTNAGG	1 cut(s)	743
BsuI	GTATCC	1 cut(s)	1369
BsuRI	GGCC	3 cut(s)	238, 247, 306
BsuTUI	ATCGAT	1 cut(s)	232
BtsCI	GGATG	5 cut(s)	68, 454, 531, 1391, 1415
BtsIMutI	CAGTG	1 cut(s)	102
BveI	ACCTGC	1 cut(s)	448
Cac8I	GCNNGC	2 cut(s)	317, 352
CfoI	GCGC	2 cut(s)	374, 816
Cfr10I	RCCGGY	1 cut(s)	277
Cfr13I	GGNCC	5 cut(s)	133, 158, 246, 361, 1475
Cfr9I	CCCGGG	1 cut(s)	248
ClaI	ATCGAT	1 cut(s)	232
CseI	GACGC	1 cut(s)	62
Csp6I	GTAC	2 cut(s)	428, 612
CviAII	CATG	1 cut(s)	1495
CviQI	GTAC	2 cut(s)	428, 612
DdeI	CTNAG	4 cut(s)	489, 743, 1125, 1401
DpnI	GATC	8 cut(s)	390, 404, 586, 619, 762, 984, 1065, 1468
DpnII	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
DraI	TTTAAA	1 cut(s)	705
EaeI	YGGCCR	2 cut(s)	236, 304
Eam1104I	CTCTTC	1 cut(s)	411
EarI	CTCTTC	1 cut(s)	411
Ecl136II	GAGCTC	2 cut(s)	319, 350
Eco24I	GRGCYC	2 cut(s)	321, 352
Eco31I	GGTCTC	1 cut(s)	335
Eco47I	GGWCC	4 cut(s)	133, 158, 361, 1475
Eco53kI	GAGCTC	2 cut(s)	319, 350
Eco57I	CTGAAG	1 cut(s)	305
Eco81I	CCTNAGG	1 cut(s)	743
Eco88I	CYCGRG	3 cut(s)	101, 248, 345
EcoICRI	GAGCTC	2 cut(s)	319, 350
EcoO109I	RGGNCCY	1 cut(s)	361
EcoRII	CCWGG	3 cut(s)	363, 830, 1477
EcoT38I	GRGCYC	2 cut(s)	321, 352
FaeI	CATG	1 cut(s)	1498
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FaqI	GGGAC	4 cut(s)	144, 266, 1089, 1263
FatI	CATG	1 cut(s)	1494
FauI	CCCGC	1 cut(s)	91
FbaI	TGATCA	1 cut(s)	760
Fnu4HI	GCNGC	8 cut(s)	42, 180, 307, 510, 663, 666, 851, 1456
FokI	GGATG	5 cut(s)	55, 461, 518, 1378, 1402
FriOI	GRGCYC	2 cut(s)	321, 352
Fsp4HI	GCNGC	8 cut(s)	42, 180, 307, 510, 663, 666, 851, 1456
GlaI	GCGC	2 cut(s)	373, 815
GluI	GCNGC	8 cut(s)	42, 180, 307, 510, 663, 666, 851, 1456
GsaI	CCCAGC	1 cut(s)	61
GsuI	CTGGAG	2 cut(s)	534, 891
HaeII	RGCGCY	2 cut(s)	375, 817
HaeIII	GGCC	3 cut(s)	238, 247, 306
HapII	CCGG	9 cut(s)	131, 174, 239, 249, 278, 535, 615, 639, 1107
HgaI	GACGC	1 cut(s)	62
HhaI	GCGC	2 cut(s)	374, 816
Hin1I	GRCGYC	1 cut(s)	54
Hin1II	CATG	1 cut(s)	1498
Hin6I	GCGC	2 cut(s)	372, 814
HinP1I	GCGC	2 cut(s)	372, 814
HincII	GTYRAC	1 cut(s)	37
HindII	GTYRAC	1 cut(s)	37
HindIII	AAGCTT	1 cut(s)	699
HinfI	GANTC	6 cut(s)	104, 152, 209, 908, 941, 1318
HpaII	CCGG	9 cut(s)	131, 174, 239, 249, 278, 535, 615, 639, 1107
HphI	GGTGA	2 cut(s)	429, 731
Hpy166II	GTNNAC	2 cut(s)	37, 1036
Hpy188I	TCNGA	7 cut(s)	73, 109, 169, 285, 1128, 1302, 1508
Hpy188III	TCNNGA	8 cut(s)	131, 143, 386, 480, 639, 920, 1092, 1322
Hpy8I	GTNNAC	2 cut(s)	37, 1036
Hpy99I	CGWCG	2 cut(s)	203, 1212
HpyAV	CCTTC	6 cut(s)	280, 963, 1017, 1038, 1077, 1178
HpyCH4III	ACNGT	4 cut(s)	97, 1234, 1270, 1337
HpyCH4IV	ACGT	3 cut(s)	198, 472, 925
HpyF10VI	GCNNNNNNNGC	9 cut(s)	47, 244, 274, 303, 312, 449, 518, 725, 734
HpyF3I	CTNAG	4 cut(s)	489, 743, 1125, 1401
HpySE526I	ACGT	3 cut(s)	198, 472, 925
Hsp92I	GRCGYC	1 cut(s)	54
Hsp92II	CATG	1 cut(s)	1498
HspAI	GCGC	2 cut(s)	372, 814
Kpn2I	TCCGGA	2 cut(s)	130, 638
KpnI	GGTACC	1 cut(s)	431
Ksp22I	TGATCA	1 cut(s)	760
Kzo9I	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
LguI	GCTCTTC	1 cut(s)	411
LmnI	GCTCC	3 cut(s)	1117, 1220, 1417
Lsp1109I	GCAGC	6 cut(s)	28, 166, 496, 649, 652, 837
LweI	GCATC	3 cut(s)	402, 439, 557
MaeII	ACGT	3 cut(s)	198, 472, 925
MaeIII	GTNAC	5 cut(s)	435, 1120, 1351, 1358, 1431
MalI	GATC	8 cut(s)	390, 404, 586, 619, 762, 984, 1065, 1468
MboI	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
MboII	GAAGA	6 cut(s)	340, 428, 612, 799, 1068, 1080
MfeI	CAATTG	1 cut(s)	935
MflI	RGATCY	3 cut(s)	617, 982, 1063
MhlI	GDGCHC	6 cut(s)	102, 321, 352, 881, 1114, 1414
MlyI	GAGTC	2 cut(s)	113, 917
MmeI	TCCRAC	2 cut(s)	132, 358
MroI	TCCGGA	2 cut(s)	130, 638
MroXI	GAANNNNTTC	1 cut(s)	29
MseI	TTAA	7 cut(s)	324, 485, 564, 569, 590, 704, 1229
MspI	CCGG	9 cut(s)	131, 174, 239, 249, 278, 535, 615, 639, 1107
MspR9I	CCNGG	8 cut(s)	175, 240, 249, 250, 365, 832, 1107, 1479
MunI	CAATTG	1 cut(s)	935
Mva1269I	GAATGC	3 cut(s)	354, 521, 1199
MvaI	CCWGG	3 cut(s)	365, 832, 1479
MwoI	GCNNNNNNNGC	9 cut(s)	47, 244, 274, 303, 312, 449, 518, 725, 734
NciI	CCSGG	5 cut(s)	175, 240, 249, 250, 1107
NdeII	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
NlaIII	CATG	1 cut(s)	1498
NlaIV	GGNNCC	7 cut(s)	160, 362, 429, 651, 1222, 1285, 1347
NmuCI	GTSAC	2 cut(s)	435, 1431
NspI	RCATGY	1 cut(s)	1498
PaqCI	CACCTGC	1 cut(s)	448
PciSI	GCTCTTC	1 cut(s)	411
PcsI	WCGNNNNNNNCGW	1 cut(s)	204
PctI	GAATGC	3 cut(s)	354, 521, 1199
PdmI	GAANNNNTTC	1 cut(s)	29
PfeI	GAWTC	4 cut(s)	152, 209, 941, 1318
PfoI	TCCNGGA	2 cut(s)	363, 1477
PkrI	GCNGC	8 cut(s)	43, 181, 308, 511, 664, 667, 852, 1457
PleI	GAGTC	2 cut(s)	112, 916
PpsI	GAGTC	2 cut(s)	112, 916
PpuMI	RGGWCCY	1 cut(s)	361
PshBI	ATTAAT	2 cut(s)	564, 590
PsiI	TTATAA	1 cut(s)	1331
Psp124BI	GAGCTC	2 cut(s)	321, 352
Psp5II	RGGWCCY	1 cut(s)	361
Psp6I	CCWGG	3 cut(s)	363, 830, 1477
PspFI	CCCAGC	1 cut(s)	57
PspGI	CCWGG	3 cut(s)	363, 830, 1477
PspN4I	GGNNCC	7 cut(s)	160, 362, 429, 651, 1222, 1285, 1347
PspPI	GGNCC	5 cut(s)	133, 158, 246, 361, 1475
PspPPI	RGGWCCY	1 cut(s)	361
PstI	CTGCAG	1 cut(s)	670
PsuI	RGATCY	3 cut(s)	617, 982, 1063
RsaI	GTAC	2 cut(s)	429, 613
RsaNI	GTAC	2 cut(s)	428, 612
SacI	GAGCTC	2 cut(s)	321, 352
SapI	GCTCTTC	1 cut(s)	411
SaqAI	TTAA	7 cut(s)	324, 485, 564, 569, 590, 704, 1229
SatI	GCNGC	8 cut(s)	42, 180, 307, 510, 663, 666, 851, 1456
Sau3AI	GATC	8 cut(s)	388, 402, 584, 617, 760, 982, 1063, 1466
Sau96I	GGNCC	5 cut(s)	133, 158, 246, 361, 1475
SchI	GAGTC	2 cut(s)	113, 917
ScrFI	CCNGG	8 cut(s)	175, 240, 249, 250, 365, 832, 1107, 1479
SduI	GDGCHC	6 cut(s)	102, 321, 352, 881, 1114, 1414
SfaNI	GCATC	3 cut(s)	402, 439, 557
SfcI	CTRYAG	4 cut(s)	666, 720, 1269, 1489
SfiI	GGCCNNNNNGGCC	1 cut(s)	244
SinI	GGWCC	4 cut(s)	133, 158, 361, 1475
SmaI	CCCGGG	1 cut(s)	250
SsiI	CCGC	9 cut(s)	9, 44, 84, 307, 728, 776, 1163, 1218, 1455
SspI	AATATT	2 cut(s)	465, 1195
SstI	GAGCTC	2 cut(s)	321, 352
StyD4I	CCNGG	8 cut(s)	173, 238, 247, 248, 363, 830, 1105, 1477
TaaI	ACNGT	4 cut(s)	97, 1234, 1270, 1337
TaiI	ACGT	3 cut(s)	201, 475, 928
TaqI	TCGA	2 cut(s)	232, 797
TauI	GCSGC	2 cut(s)	309, 1458
TfiI	GAWTC	4 cut(s)	152, 209, 941, 1318
Tru1I	TTAA	7 cut(s)	324, 485, 564, 569, 590, 704, 1229
Tru9I	TTAA	7 cut(s)	324, 485, 564, 569, 590, 704, 1229
TscAI	CASTG	1 cut(s)	102
TseFI	GTSAC	2 cut(s)	435, 1431
TseI	GCWGC	6 cut(s)	41, 179, 509, 662, 665, 850
Tsp45I	GTSAC	2 cut(s)	435, 1431
TspDTI	ATGAA	2 cut(s)	1192, 1275
TspGWI	ACGGA	2 cut(s)	642, 1073
TspMI	CCCGGG	1 cut(s)	248
TspRI	CASTG	1 cut(s)	102
VpaK11BI	GGWCC	4 cut(s)	133, 158, 361, 1475
VspI	ATTAAT	2 cut(s)	564, 590
XapI	RAATTY	2 cut(s)	963, 1146
XceI	RCATGY	1 cut(s)	1498
XcmI	CCANNNNNNNNNTGG	2 cut(s)	538, 828
XmaI	CCCGGG	1 cut(s)	248
XmnI	GAANNNNTTC	1 cut(s)	29

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.