MD15G1158100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003311
Physical Location & Seq
Forward (+)
11742831 .. 11743454
624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1158100.v1.1.491

Sequence Viewer

Length: 624 bp
ATGAGGACGGCGATTCTCCGATCGCAGGATTGCCTCCGAGGTCGCTTCCGCCACGAAGCCCTAACCCTAACCCTCTCCCCCCGCTCCGGATCACGAAGGAGCTCCAATTTCTCTAACCCTAACCCTAATTCCACCCCCAACCATGTCTCCACCGCCAATATTCATCAATCTCGCCGCCGGAAGAGGAGTCCGATGGGGCTTCAGTCCAATCAACACGAACGGCCTCGCGATTGGTGCTCTGATCGCGCCGCGGTTGCGAAGGCGCCGGGGAAAAACCTGGTGATTGGACAGGTCAAGATCCTGAGACGCGGCGAGGATTTGAACCCAGAGAAGAATAACCGAGGGCTCGGAGTTGCTGCCCGCGATGATCGCAAGCCGAAGGCGATGAAGGAAAACGATCCGGATTTGGTTTTGGGATCCACGGACCGTTTGGGACCCGACCCGGAGACCGTGCAGAAGCAGATTAAGGCTGCGGAGTTCAAAGTTATGGATGCGATTTACGCCGGATCGAGCGCTTTCTGCGCGTCTCCGCCTCCGAGCTCGGTGCCACTGCCTAGATTTTTGGGAAGGAACGGAACCGCCACCAGCGATCTCCGCAGGCTGCTGCGACTCGATTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNRC

Protein Analysis

208

Amino Acids

22.92

Weight (kDa)

10.97

Isoelectric Point (pI)

64.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 544
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
AccIII TCCGGA 2 cut(s) 86, 400
AclWI GGATC 6 cut(s) 97, 292, 392, 411, 424, 514
AcuI CTGAAG 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 263
AfeI AGCGCT 1 cut(s) 514
AfiI CCNNNNNNNGG 2 cut(s) 25, 86
AgsI TTSAA 2 cut(s) 322, 481
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 2 cut(s) 102, 540
AluI AGCT 2 cut(s) 102, 540
Alw21I GWGCWC 3 cut(s) 104, 239, 542
Alw26I GTCTC 4 cut(s) 151, 298, 440, 531
AlwI GGATC 6 cut(s) 97, 292, 392, 411, 424, 514
Aor13HI TCCGGA 2 cut(s) 86, 400
Aor51HI AGCGCT 1 cut(s) 514
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 4 cut(s) 356, 470, 601, 604
AspLEI GCGC 4 cut(s) 248, 265, 515, 524
AspS9I GGNCC 2 cut(s) 424, 434
AsuC2I CCSGG 2 cut(s) 267, 443
AsuHPI GGTGA 1 cut(s) 292
AvaII GGWCC 2 cut(s) 424, 434
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 2 cut(s) 262, 544
BanII GRGCYC 3 cut(s) 104, 348, 542
Bbv12I GWGCWC 3 cut(s) 104, 239, 542
BbvI GCAGC 4 cut(s) 343, 457, 588, 591
BccI CCATC 1 cut(s) 187
BceAI ACGGC 2 cut(s) 24, 236
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BciT130I CCWGG 1 cut(s) 278
BcnI CCSGG 2 cut(s) 267, 443
BcoDI GTCTC 4 cut(s) 151, 298, 440, 531
BfaI CTAG 1 cut(s) 555
BfoI RGCGCY 2 cut(s) 266, 516
BisI GCNGC 7 cut(s) 175, 249, 310, 357, 471, 602, 605
BlsI GCNGC 7 cut(s) 176, 250, 311, 358, 472, 603, 606
Bme1390I CCNGG 3 cut(s) 267, 278, 443
Bme18I GGWCC 2 cut(s) 424, 434
BmgT120I GGNCC 2 cut(s) 424, 434
BmiI GGNNCC 6 cut(s) 264, 418, 435, 436, 546, 577
BmrFI CCNGG 3 cut(s) 267, 278, 443
BmsI GCATC 1 cut(s) 481
BpuMI CCSGG 2 cut(s) 267, 443
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 5 cut(s) 37, 249, 266, 340, 420
BsaWI WCCGGW 2 cut(s) 86, 400
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 86
BseAI TCCGGA 2 cut(s) 86, 400
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 5 cut(s) 37, 249, 266, 340, 420
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 2 cut(s) 25, 86
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 343, 457, 588, 591
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 223
BshNI GGYRCC 2 cut(s) 262, 544
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 3 cut(s) 104, 239, 542
BsiSI CCGG 6 cut(s) 87, 178, 266, 401, 443, 504
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 2 cut(s) 25, 86
BsmAI GTCTC 4 cut(s) 151, 298, 440, 531
BsmBI CGTCTC 2 cut(s) 298, 531
BsmFI GGGAC 1 cut(s) 447
BsnI GGCC 1 cut(s) 223
Bso31I GGTCTC 1 cut(s) 440
Bsp1286I GDGCHC 4 cut(s) 104, 239, 348, 542
Bsp13I TCCGGA 2 cut(s) 86, 400
Bsp143I GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
Bsp68I TCGCGA 1 cut(s) 228
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 1 cut(s) 294
BspEI TCCGGA 2 cut(s) 86, 400
BspFNI CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
BspLI GGNNCC 6 cut(s) 264, 418, 435, 436, 546, 577
BspPI GGATC 6 cut(s) 97, 292, 392, 411, 424, 514
BspT107I GGYRCC 2 cut(s) 262, 544
BspTNI GGTCTC 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 84
BssECI CCNNGG 5 cut(s) 37, 249, 266, 340, 420
BssMI GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
BssNI GRCGYC 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 428, 451
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 3 cut(s) 361, 374, 599
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 2 cut(s) 249, 420
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
BstH2I RGCGCY 2 cut(s) 266, 516
BstHHI GCGC 4 cut(s) 248, 265, 515, 524
BstKTI GATC 9 cut(s) 23, 92, 244, 300, 370, 400, 419, 509, 592
BstMAI GTCTC 4 cut(s) 151, 298, 440, 531
BstMBI GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 8 cut(s) 234, 243, 254, 369, 500, 519, 521, 594
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 3 cut(s) 265, 276, 441
BstUI CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
BstV1I GCAGC 4 cut(s) 343, 457, 588, 591
BstX2I RGATCY 2 cut(s) 297, 416
BstYI RGATCY 2 cut(s) 297, 416
BsuRI GGCC 1 cut(s) 223
BtgI CCRYGG 2 cut(s) 249, 420
BtgZI GCGATG 2 cut(s) 378, 398
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 1 cut(s) 548
BtuMI TCGCGA 1 cut(s) 228
Cac8I GCNNGC 3 cut(s) 361, 374, 599
CfoI GCGC 4 cut(s) 248, 265, 515, 524
Cfr13I GGNCC 2 cut(s) 424, 434
Cfr42I CCGCGG 1 cut(s) 252
CpoI CGGWCCG 1 cut(s) 424
CseI GACGC 2 cut(s) 315, 513
CsiI ACCWGGT 1 cut(s) 276
CspI CGGWCCG 1 cut(s) 424
CviAII CATG 1 cut(s) 143
CviJI RGCY 9 cut(s) 59, 102, 199, 223, 346, 376, 470, 540, 601
CviKI_1 RGCY 9 cut(s) 59, 102, 199, 223, 346, 376, 470, 540, 601
DdeI CTNAG 1 cut(s) 302
DinI GGCGCC 1 cut(s) 264
DpnI GATC 9 cut(s) 22, 91, 243, 299, 369, 399, 418, 508, 591
DpnII GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 2 cut(s) 38, 519
Ecl136II GAGCTC 2 cut(s) 102, 540
Eco24I GRGCYC 3 cut(s) 104, 348, 542
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 424, 434
Eco47III AGCGCT 1 cut(s) 514
Eco53kI GAGCTC 2 cut(s) 102, 540
Eco57I CTGAAG 1 cut(s) 185
EcoICRI GAGCTC 2 cut(s) 102, 540
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 276
EcoT38I GRGCYC 3 cut(s) 104, 348, 542
EgeI GGCGCC 1 cut(s) 264
EheI GGCGCC 1 cut(s) 264
Esp3I CGTCTC 2 cut(s) 298, 531
FaeI CATG 1 cut(s) 146
FaiI YATR 2 cut(s) 144, 488
FaqI GGGAC 1 cut(s) 447
FatI CATG 1 cut(s) 142
FauI CCCGC 2 cut(s) 89, 368
Fnu4HI GCNGC 7 cut(s) 175, 249, 310, 357, 471, 602, 605
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 3 cut(s) 104, 348, 542
Fsp4HI GCNGC 7 cut(s) 175, 249, 310, 357, 471, 602, 605
FspBI CTAG 1 cut(s) 555
GlaI GCGC 4 cut(s) 247, 264, 514, 523
GluI GCNGC 7 cut(s) 175, 249, 310, 357, 471, 602, 605
HaeII RGCGCY 2 cut(s) 266, 516
HaeIII GGCC 1 cut(s) 223
HapII CCGG 6 cut(s) 87, 178, 266, 401, 443, 504
HgaI GACGC 2 cut(s) 315, 513
HhaI GCGC 4 cut(s) 248, 265, 515, 524
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 1 cut(s) 146
Hin6I GCGC 4 cut(s) 246, 263, 513, 522
HinP1I GCGC 4 cut(s) 246, 263, 513, 522
HinfI GANTC 3 cut(s) 13, 187, 609
HpaII CCGG 6 cut(s) 87, 178, 266, 401, 443, 504
HphI GGTGA 1 cut(s) 292
Hpy188I TCNGA 6 cut(s) 20, 38, 192, 241, 350, 537
Hpy188III TCNNGA 6 cut(s) 87, 93, 227, 295, 301, 401
HpyAV CCTTC 5 cut(s) 90, 253, 373, 382, 561
HpyCH4III ACNGT 2 cut(s) 428, 451
HpyCH4V TGCA 1 cut(s) 454
HpyF10VI GCNNNNNNNGC 8 cut(s) 234, 243, 254, 369, 500, 519, 521, 594
HpyF3I CTNAG 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 1 cut(s) 146
HspAI GCGC 4 cut(s) 246, 263, 513, 522
KasI GGCGCC 1 cut(s) 262
KflI GGGWCCC 1 cut(s) 434
Kpn2I TCCGGA 2 cut(s) 86, 400
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
LmnI GCTCC 3 cut(s) 89, 99, 107
Lsp1109I GCAGC 4 cut(s) 343, 457, 588, 591
LweI GCATC 1 cut(s) 481
MabI ACCWGGT 1 cut(s) 276
MaeI CTAG 1 cut(s) 555
MalI GATC 9 cut(s) 22, 91, 243, 299, 369, 399, 418, 508, 591
MbiI CCGCTC 1 cut(s) 84
MboI GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
MboII GAAGA 2 cut(s) 193, 343
MflI RGATCY 2 cut(s) 297, 416
MhlI GDGCHC 4 cut(s) 104, 239, 348, 542
MluCI AATT 2 cut(s) 106, 127
Mly113I GGCGCC 1 cut(s) 263
MlyI GAGTC 2 cut(s) 196, 603
MnlI CCTC 8 cut(s) 32, 44, 83, 177, 234, 307, 335, 543
MroI TCCGGA 2 cut(s) 86, 400
MseI TTAA 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 6 cut(s) 87, 178, 266, 401, 443, 504
MspR9I CCNGG 3 cut(s) 267, 278, 443
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 6 cut(s) 228, 246, 251, 309, 363, 524
MwoI GCNNNNNNNGC 8 cut(s) 234, 243, 254, 369, 500, 519, 521, 594
NarI GGCGCC 1 cut(s) 263
NciI CCSGG 2 cut(s) 267, 443
NdeII GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 6 cut(s) 264, 418, 435, 436, 546, 577
NruI TCGCGA 1 cut(s) 228
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 7 cut(s) 176, 250, 311, 358, 472, 603, 606
Ple19I CGATCG 1 cut(s) 23
PleI GAGTC 2 cut(s) 195, 603
PluTI GGCGCC 1 cut(s) 266
PpsI GAGTC 2 cut(s) 195, 603
PpuMI RGGWCCY 1 cut(s) 434
Psp124BI GAGCTC 2 cut(s) 104, 542
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 6 cut(s) 264, 418, 435, 436, 546, 577
PspPI GGNCC 2 cut(s) 424, 434
PspPPI RGGWCCY 1 cut(s) 434
PsuI RGATCY 2 cut(s) 297, 416
PvuI CGATCG 1 cut(s) 23
RruI TCGCGA 1 cut(s) 228
Rsr2I CGGWCCG 1 cut(s) 424
RsrII CGGWCCG 1 cut(s) 424
SacI GAGCTC 2 cut(s) 104, 542
SacII CCGCGG 1 cut(s) 252
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 7 cut(s) 175, 249, 310, 357, 471, 602, 605
Sau3AI GATC 9 cut(s) 20, 89, 241, 297, 367, 397, 416, 506, 589
Sau96I GGNCC 2 cut(s) 424, 434
SchI GAGTC 2 cut(s) 196, 603
ScrFI CCNGG 3 cut(s) 267, 278, 443
SduI GDGCHC 4 cut(s) 104, 239, 348, 542
SetI ASST 5 cut(s) 43, 104, 279, 294, 542
SexAI ACCWGGT 1 cut(s) 276
SfaNI GCATC 1 cut(s) 481
SfoI GGCGCC 1 cut(s) 264
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 2 cut(s) 424, 434
Sse9I AATT 2 cut(s) 106, 127
SspDI GGCGCC 1 cut(s) 262
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 2 cut(s) 104, 542
StyD4I CCNGG 3 cut(s) 265, 276, 441
TaaI ACNGT 2 cut(s) 428, 451
TaqI TCGA 2 cut(s) 509, 612
TasI AATT 2 cut(s) 106, 127
TauI GCSGC 3 cut(s) 177, 251, 312
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 555
TseI GCWGC 4 cut(s) 356, 470, 601, 604
TspDTI ATGAA 2 cut(s) 152, 401
TspGWI ACGGA 2 cut(s) 437, 588
TspRI CASTG 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 424, 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.