Rmu_sc0003219.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003219.1
OG0003311
Physical Location & Seq
Reverse (-)
68343 .. 68778
436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003219.1_g000015.1.cds

Sequence Viewer

Length: 375 bp
atgaagtccgacaatcagagccttagccctaaccttgaccctacgctccggcgcggtgaggctttgaatccggcaacggaagatgagagagagggcggagttggaagagatgatcggaaggcgagggaggagagcgtggagtgttcggattcggatctggtgttgggatccacggaaaggttgggacccgacccggagacggtgcagaagcagatcaaggttgtagagttcaaggtgatggatgcgatctacgctggatcgggggcttattatgcttctccgccgccgagctccattccgctgccggcttttttggctaagtacggtgcagcgacaagcaatctgcgccggctgctgcgtctagatttggtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNRC

Protein Analysis

124

Amino Acids

13.48

Weight (kDa)

4.88

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 54
AciI CCGC 5 cut(s) 54, 96, 281, 284, 299
AclWI GGATC 4 cut(s) 162, 162, 175, 265
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 177, 199
AgsI TTSAA 2 cut(s) 67, 232
AluBI AGCT 1 cut(s) 291
AluI AGCT 1 cut(s) 291
Alw21I GWGCWC 1 cut(s) 293
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 4 cut(s) 162, 162, 175, 265
ApeKI GCWGC 4 cut(s) 301, 329, 352, 355
AspLEI GCGC 2 cut(s) 54, 348
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 68, 247
AvaII GGWCC 1 cut(s) 185
BamHI GGATCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 293
BbvI GCAGC 4 cut(s) 288, 339, 341, 342
BccI CCATC 1 cut(s) 232
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 362
BisI GCNGC 5 cut(s) 284, 302, 330, 353, 356
BlsI GCNGC 5 cut(s) 285, 303, 331, 354, 357
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 169, 186, 187
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 232
Bpu10I CCTNAGC 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 199
Bse118I RCCGGY 2 cut(s) 304, 348
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 177, 199
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 4 cut(s) 288, 339, 341, 342
BsgI GTGCAG 2 cut(s) 224, 348
Bsh1236I CGCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 293
BsiSI CCGG 5 cut(s) 49, 71, 194, 305, 349
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 177, 199
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 6 cut(s) 112, 154, 167, 213, 246, 257
BspACI CCGC 5 cut(s) 54, 96, 281, 284, 299
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 3 cut(s) 169, 186, 187
BspPI GGATC 4 cut(s) 162, 162, 175, 265
BsrFI RCCGGY 2 cut(s) 304, 348
BssAI RCCGGY 2 cut(s) 304, 348
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 6 cut(s) 112, 154, 167, 213, 246, 257
Bst4CI ACNGT 2 cut(s) 202, 326
Bst6I CTCTTC 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 306, 350
BstDEI CTNAG 2 cut(s) 23, 318
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 54
BstHHI GCGC 2 cut(s) 54, 348
BstKTI GATC 6 cut(s) 115, 157, 170, 216, 249, 260
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 6 cut(s) 112, 154, 167, 213, 246, 257
BstMWI GCNNNNNNNGC 5 cut(s) 251, 272, 314, 345, 352
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 4 cut(s) 288, 339, 341, 342
BstX2I RGATCY 2 cut(s) 154, 167
BstYI RGATCY 2 cut(s) 154, 167
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 306, 350
CfoI GCGC 2 cut(s) 54, 348
Cfr10I RCCGGY 2 cut(s) 304, 348
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 322
CviJI RGCY 8 cut(s) 21, 27, 62, 266, 291, 308, 317, 352
CviKI_1 RGCY 8 cut(s) 21, 27, 62, 266, 291, 308, 317, 352
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 2 cut(s) 23, 318
DpnI GATC 6 cut(s) 114, 156, 169, 215, 248, 259
DpnII GATC 6 cut(s) 112, 154, 167, 213, 246, 257
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
EciI GGCGGA 2 cut(s) 111, 270
Ecl136II GAGCTC 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 185
EcoT38I GRGCYC 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 191
FaiI YATR 1 cut(s) 273
FaqI GGGAC 1 cut(s) 198
Fnu4HI GCNGC 5 cut(s) 284, 302, 330, 353, 356
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 293
Fsp4HI GCNGC 5 cut(s) 284, 302, 330, 353, 356
FspBI CTAG 1 cut(s) 362
GlaI GCGC 2 cut(s) 53, 347
GluI GCNGC 5 cut(s) 284, 302, 330, 353, 356
HapII CCGG 5 cut(s) 49, 71, 194, 305, 349
HgaI GACGC 1 cut(s) 347
HhaI GCGC 2 cut(s) 54, 348
Hin6I GCGC 2 cut(s) 52, 346
HinP1I GCGC 2 cut(s) 52, 346
HinfI GANTC 2 cut(s) 67, 149
HpaII CCGG 5 cut(s) 49, 71, 194, 305, 349
HphI GGTGA 2 cut(s) 68, 247
Hpy188I TCNGA 5 cut(s) 10, 18, 117, 148, 154
Hpy188III TCNNGA 1 cut(s) 362
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 202, 326
HpyCH4V TGCA 2 cut(s) 205, 329
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 272, 314, 345, 352
HpyF3I CTNAG 2 cut(s) 23, 318
HspAI GCGC 2 cut(s) 52, 346
KflI GGGWCCC 1 cut(s) 185
KroI GCCGGC 2 cut(s) 304, 348
KroNI GCCGGC 2 cut(s) 306, 350
Kzo9I GATC 6 cut(s) 112, 154, 167, 213, 246, 257
LmnI GCTCC 2 cut(s) 51, 296
LpnPI CCDG 7 cut(s) 62, 84, 143, 207, 240, 318, 362
Lsp1109I GCAGC 4 cut(s) 288, 339, 341, 342
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 362
MalI GATC 6 cut(s) 114, 156, 169, 215, 248, 259
MboI GATC 6 cut(s) 112, 154, 167, 213, 246, 257
MboII GAAGA 2 cut(s) 92, 117
MflI RGATCY 2 cut(s) 154, 167
MhlI GDGCHC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 33, 82
MnlI CCTC 4 cut(s) 52, 85, 117, 121
MroNI GCCGGC 2 cut(s) 304, 348
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 5 cut(s) 49, 71, 194, 305, 349
MspR9I CCNGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 5 cut(s) 251, 272, 314, 345, 352
NaeI GCCGGC 2 cut(s) 306, 350
NciI CCSGG 1 cut(s) 194
NdeII GATC 6 cut(s) 112, 154, 167, 213, 246, 257
NgoMIV GCCGGC 2 cut(s) 304, 348
NlaIV GGNNCC 3 cut(s) 169, 186, 187
NmeAIII GCCGAG 1 cut(s) 312
PdiI GCCGGC 2 cut(s) 306, 350
PfeI GAWTC 2 cut(s) 67, 149
PkrI GCNGC 5 cut(s) 285, 303, 331, 354, 357
PpuMI RGGWCCY 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 185
PspN4I GGNNCC 3 cut(s) 169, 186, 187
PspPI GGNCC 1 cut(s) 185
PspPPI RGGWCCY 1 cut(s) 185
PsuI RGATCY 2 cut(s) 154, 167
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SacI GAGCTC 1 cut(s) 293
SatI GCNGC 5 cut(s) 284, 302, 330, 353, 356
Sau3AI GATC 6 cut(s) 112, 154, 167, 213, 246, 257
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 293
SetI ASST 5 cut(s) 36, 182, 222, 237, 293
SfaNI GCATC 1 cut(s) 232
SinI GGWCC 1 cut(s) 185
SsiI CCGC 5 cut(s) 54, 96, 281, 284, 299
SspMI CTAG 1 cut(s) 362
SstI GAGCTC 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 202, 326
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 2 cut(s) 67, 149
TseI GCWGC 4 cut(s) 301, 329, 352, 355
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 92, 188
VpaK11BI GGWCC 1 cut(s) 185
XbaI TCTAGA 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.