MD16G1055200.v1.1

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003016
Physical Location & Seq
Reverse (-)
3891161 .. 3893636
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055200.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGAAGACTTATGGAAGCGAGCGAAGACCTTCGCCGAAGAAGCGGCCAAGAAATCCCAGGCCCTAACCACCTCCGCCAAGATCACCGACCTAGTTTCCGAAACCGCCAAGCGATCCCGCGAGTTCGCCGCCGAGGCGTCCAAGAAGGCCGACGAGATCAAGACAGCGGCGCTTAAGCAGGCCGATCAGATCAAGTCCCTCAACGTCTCCGAGATGATACCTCCCCAGCTTGCCTCGCTCTCGATCGTCAACTCCGCCTCCGCCTCCGCCTCCTCCGCCCCTGAGCCGCCGTCGCCGTTGGAGCTTAAGAAGTTTGGTGTCACCGAGGATCTCAGAGATTTTGTCCAGGGACTCACTTCCGATACCTTCAAGCATTTTCCGGTCCAAGATGCGGCAGAGGAGGTCTCCGATGTGCCGACGACGGCATCGAATGTGAGGAAAGATCTGACGGAGTGGCAGGAGCGGCATGCGACTTTGGTGCTTACTACTGTCAAGGAAATTTCAAGGCTGAGGTATGAATTGTGTCCGCGAGTTATGAAAGAAAGAAGATTCTGGAAGATATATTTTACCCTTGTGAGCAGTCATGTGGCTCCATATGAGAAGCAATACATGGAGGAATTGAAGGTCCAGGAAGCAGAGAAACTAAAAGATGATATTGTGAAGCCAACGCCTCCTGTTGGAGGAACTGACAAAGCGGAAGGATCTGAAAAGAACACGAAGCCTTCAGTTTCAAAATCATCGTCAGCTGAACAGGACTTGGATAGTTTCCTTTTGGGTGATCTTGAAGACAGTGATGGGGGTCCGGATGATCGTGATGATGGTGATCGGAGCTTTGATGATGATTTTGACAAGATCGACAATTCGGATGTCGAAGATGAGAAGCACGAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

299

Amino Acids

33.22

Weight (kDa)

4.87

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 149 - 196 2e-11 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 463
AccII CGCG 2 cut(s) 120, 529
AccIII TCCGGA 1 cut(s) 802
AclWI GGATC 3 cut(s) 108, 336, 709
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 137
AfiI CCNNNNNNNGG 3 cut(s) 391, 677, 680
AflII CTTAAG 2 cut(s) 173, 305
AgsI TTSAA 5 cut(s) 370, 504, 622, 732, 785
AjnI CCWGG 3 cut(s) 57, 345, 627
AluBI AGCT 4 cut(s) 229, 304, 746, 831
AluI AGCT 4 cut(s) 229, 304, 746, 831
Alw26I GTCTC 2 cut(s) 211, 409
AlwI GGATC 3 cut(s) 108, 336, 709
Aor13HI TCCGGA 1 cut(s) 802
AoxI GGCC 4 cut(s) 45, 60, 147, 180
ApoI RAATTY 1 cut(s) 498
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 4 cut(s) 61, 382, 625, 800
AsuHPI GGTGA 4 cut(s) 76, 313, 788, 833
AvaII GGWCC 3 cut(s) 382, 625, 800
BauI CACGAG 1 cut(s) 884
BbsI GAAGAC 3 cut(s) 12, 32, 792
BbvCI CCTCAGC 1 cut(s) 509
BccI CCATC 2 cut(s) 788, 812
BceAI ACGGC 3 cut(s) 274, 280, 438
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 3 cut(s) 59, 347, 629
BcoDI GTCTC 2 cut(s) 211, 409
BfaI CTAG 1 cut(s) 92
BfoI RGCGCY 1 cut(s) 173
BfrI CTTAAG 2 cut(s) 173, 305
BglI GCCNNNNNGGC 1 cut(s) 134
BglII AGATCT 1 cut(s) 442
BisI GCNGC 6 cut(s) 45, 129, 168, 287, 393, 464
BlsI GCNGC 6 cut(s) 46, 130, 169, 288, 394, 465
Bme1390I CCNGG 3 cut(s) 59, 347, 629
Bme18I GGWCC 3 cut(s) 382, 625, 800
BmgT120I GGNCC 4 cut(s) 61, 382, 625, 800
BmiI GGNNCC 2 cut(s) 591, 801
BmrFI CCNGG 3 cut(s) 59, 347, 629
BmsI GCATC 2 cut(s) 379, 434
BpiI GAAGAC 3 cut(s) 12, 32, 792
BplI GAGNNNNNCTC 2 cut(s) 389, 421
Bpu10I CCTNAGC 2 cut(s) 282, 509
BsaBI GATNNNNATC 1 cut(s) 822
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 4 cut(s) 57, 132, 324, 346
BsaWI WCCGGW 2 cut(s) 379, 802
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 677, 680
Bse8I GATNNNNATC 1 cut(s) 822
BseAI TCCGGA 1 cut(s) 802
BseBI CCWGG 3 cut(s) 59, 347, 629
BseDI CCNNGG 4 cut(s) 57, 132, 324, 346
BseGI GGATG 2 cut(s) 811, 871
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 3 cut(s) 391, 677, 680
BseMII CTCAG 3 cut(s) 273, 346, 500
BseRI GAGGAG 2 cut(s) 262, 413
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 2 cut(s) 120, 529
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 47, 62, 149, 182
BsiEI CGRYCG 1 cut(s) 246
BsiSI CCGG 2 cut(s) 380, 803
BslFI GGGAC 2 cut(s) 181, 363
BslI CCNNNNNNNGG 3 cut(s) 391, 677, 680
BsmAI GTCTC 2 cut(s) 211, 409
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 2 cut(s) 181, 363
BsnI GGCC 4 cut(s) 47, 62, 149, 182
Bso31I GGTCTC 1 cut(s) 409
Bsp13I TCCGGA 1 cut(s) 802
BspANI GGCC 4 cut(s) 47, 62, 149, 182
BspCNI CTCAG 3 cut(s) 274, 345, 501
BspEI TCCGGA 1 cut(s) 802
BspFNI CGCG 2 cut(s) 120, 529
BspLI GGNNCC 2 cut(s) 591, 801
BspPI GGATC 3 cut(s) 108, 336, 709
BspTI CTTAAG 2 cut(s) 173, 305
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 463
BssECI CCNNGG 4 cut(s) 57, 132, 324, 346
BssNI GRCGYC 1 cut(s) 137
BssSI CACGAG 1 cut(s) 884
Bst2BI CACGAG 1 cut(s) 884
Bst2UI CCWGG 3 cut(s) 59, 347, 629
Bst4CI ACNGT 2 cut(s) 490, 791
BstACI GRCGYC 1 cut(s) 137
BstAFI CTTAAG 2 cut(s) 173, 305
BstC8I GCNNGC 4 cut(s) 21, 180, 231, 468
BstDEI CTNAG 3 cut(s) 282, 332, 509
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 2 cut(s) 811, 871
BstFNI CGCG 2 cut(s) 120, 529
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 211, 409
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 7 cut(s) 41, 134, 235, 275, 292, 301, 463
BstNI CCWGG 3 cut(s) 59, 347, 629
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 57, 345, 627
BstUI CGCG 2 cut(s) 120, 529
BstV2I GAAGAC 3 cut(s) 12, 32, 792
BstX2I RGATCY 3 cut(s) 328, 442, 701
BstYI RGATCY 3 cut(s) 328, 442, 701
BsuRI GGCC 4 cut(s) 47, 62, 149, 182
BtsCI GGATG 2 cut(s) 811, 871
BtsIMutI CAGTG 1 cut(s) 796
Cac8I GCNNGC 4 cut(s) 21, 180, 231, 468
CfoI GCGC 1 cut(s) 172
Cfr13I GGNCC 4 cut(s) 61, 382, 625, 800
CseI GACGC 1 cut(s) 126
CviAII CATG 3 cut(s) 467, 584, 610
DdeI CTNAG 3 cut(s) 282, 332, 509
EaeI YGGCCR 1 cut(s) 45
EciI GGCGGA 5 cut(s) 64, 244, 250, 256, 265
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 382, 625, 800
Eco57I CTGAAG 1 cut(s) 708
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 3 cut(s) 57, 345, 627
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 3 cut(s) 470, 587, 613
FaiI YATR 9 cut(s) 13, 468, 516, 536, 562, 585, 595, 597, 611
FaqI GGGAC 2 cut(s) 181, 363
FatI CATG 3 cut(s) 466, 583, 609
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 6 cut(s) 45, 129, 168, 287, 393, 464
FokI GGATG 2 cut(s) 818, 878
Fsp4HI GCNGC 6 cut(s) 45, 129, 168, 287, 393, 464
FspBI CTAG 1 cut(s) 92
GlaI GCGC 1 cut(s) 171
GluI GCNGC 6 cut(s) 45, 129, 168, 287, 393, 464
GsaI CCCAGC 1 cut(s) 229
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 4 cut(s) 47, 62, 149, 182
HapII CCGG 2 cut(s) 380, 803
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 172
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 3 cut(s) 470, 587, 613
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 2 cut(s) 351, 549
HpaII CCGG 2 cut(s) 380, 803
HphI GGTGA 4 cut(s) 76, 313, 788, 833
Hpy166II GTNNAC 1 cut(s) 250
Hpy188III TCNNGA 6 cut(s) 160, 241, 553, 782, 803, 812
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 4 cut(s) 155, 295, 421, 424
HpyAV CCTTC 6 cut(s) 40, 139, 376, 616, 692, 732
HpyCH4III ACNGT 2 cut(s) 490, 791
HpyCH4IV ACGT 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 134, 235, 275, 292, 301, 463
HpyF3I CTNAG 3 cut(s) 282, 332, 509
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 3 cut(s) 470, 587, 613
HspAI GCGC 1 cut(s) 170
Kpn2I TCCGGA 1 cut(s) 802
LmnI GCTCC 4 cut(s) 301, 460, 595, 828
LweI GCATC 2 cut(s) 379, 434
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 463
MboII GAAGA 7 cut(s) 17, 37, 50, 558, 568, 797, 884
MflI RGATCY 3 cut(s) 328, 442, 701
MluCI AATT 4 cut(s) 498, 518, 617, 859
MlyI GAGTC 1 cut(s) 345
MmeI TCCRAC 2 cut(s) 279, 658
MroI TCCGGA 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 2 cut(s) 174, 306
MspA1I CMGCKG 2 cut(s) 167, 746
MspCI CTTAAG 2 cut(s) 173, 305
MspI CCGG 2 cut(s) 380, 803
MspR9I CCNGG 3 cut(s) 59, 347, 629
MvaI CCWGG 3 cut(s) 59, 347, 629
MvnI CGCG 2 cut(s) 120, 529
MwoI GCNNNNNNNGC 7 cut(s) 41, 134, 235, 275, 292, 301, 463
NdeI CATATG 1 cut(s) 595
NlaIII CATG 3 cut(s) 470, 587, 613
NlaIV GGNNCC 2 cut(s) 591, 801
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 1 cut(s) 549
PfoI TCCNGGA 1 cut(s) 627
PkrI GCNGC 6 cut(s) 46, 130, 169, 288, 394, 465
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 57, 345, 627
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 3 cut(s) 57, 345, 627
PspN4I GGNNCC 2 cut(s) 591, 801
PspPI GGNCC 4 cut(s) 61, 382, 625, 800
PsuI RGATCY 3 cut(s) 328, 442, 701
PvuI CGATCG 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 746
SaqAI TTAA 2 cut(s) 174, 306
SatI GCNGC 6 cut(s) 45, 129, 168, 287, 393, 464
Sau96I GGNCC 4 cut(s) 61, 382, 625, 800
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 3 cut(s) 59, 347, 629
SfaNI GCATC 2 cut(s) 379, 434
SinI GGWCC 3 cut(s) 382, 625, 800
SmlI CTYRAG 2 cut(s) 173, 305
SmoI CTYRAG 2 cut(s) 173, 305
SphI GCATGC 1 cut(s) 470
Sse9I AATT 4 cut(s) 498, 518, 617, 859
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 3 cut(s) 57, 345, 627
TaaI ACNGT 2 cut(s) 490, 791
TaiI ACGT 1 cut(s) 207
TaqI TCGA 4 cut(s) 242, 428, 855, 870
TasI AATT 4 cut(s) 498, 518, 617, 859
TauI GCSGC 6 cut(s) 47, 131, 170, 289, 395, 466
TfiI GAWTC 1 cut(s) 549
Tru1I TTAA 2 cut(s) 174, 306
Tru9I TTAA 2 cut(s) 174, 306
TscAI CASTG 1 cut(s) 796
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 531, 551
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 1 cut(s) 796
Vha464I CTTAAG 2 cut(s) 173, 305
VpaK11BI GGWCC 3 cut(s) 382, 625, 800
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 1 cut(s) 498
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.