Rh4AG354700

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003016
Physical Location & Seq
Reverse (-)
66320292 .. 66323091
2800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG354700.1

Sequence Viewer

Length: 489 bp
ATGTGCGTTGCTGGATTTGGTTTAGATGAAGCGGAGGAGGTTTCTGAGGTGCCGACTAAGGCGTCGAATGTGAGGAAGGATCTGACTGAGTGGCAGGAGCGCCATGCCACTCTGGTTCTCACTACTGTTAAGGAGATTTCGAGGTTAAGATATGAACTGTGCCCACGTGTTATGAAAGAAGGAAGATTCTGGAGGATATATTTTACCCTTGTGAGCAGTCATGTGGCTCCATATGAGAAGCAATATATGGAGGAGTTGAAGCTCAAGGAAGCAGAGCAATTGGAAGATGATAAAGCGAAGCAAACACCTGTTGTTGGAGAGGCTGATAAAGCAGAAGGAAGTGAAACAAACAAGAAGAGCTTAACTTCCAAATCTTCATCAACTGAGCAGGACTTGGATACATTCCTTTTGGGGGATCTTGAAGACAGCGATGGGGGTCAAGGTCCAGTTCCTTTACCTTTTGACTTGTCAAGTTCTATAGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

162

Amino Acids

18.16

Weight (kDa)

4.71

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 28 - 75 4.6e-12 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 1 cut(s) 32
AclWI GGATC 2 cut(s) 87, 423
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 314, 412
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 259, 422
AluBI AGCT 2 cut(s) 262, 360
AluI AGCT 2 cut(s) 262, 360
AlwI GGATC 2 cut(s) 87, 423
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 443
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 49
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 429
BccI CCATC 1 cut(s) 425
BciVI GTATCC 1 cut(s) 391
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 103
BfuI GTATCC 1 cut(s) 391
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 2 cut(s) 51, 228
BpiI GAAGAC 1 cut(s) 429
BpmI CTGGAG 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 62
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 412
Bse1I ACTGG 1 cut(s) 446
BseLI CCNNNNNNNGG 2 cut(s) 314, 412
BseMII CTCAG 3 cut(s) 36, 78, 375
BseNI ACTGG 1 cut(s) 446
BseRI GAGGAG 2 cut(s) 50, 266
BseSI GKGCMC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 49
BslI CCNNNNNNNGG 2 cut(s) 314, 412
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 79, 415
BspACI CCGC 1 cut(s) 32
BspCNI CTCAG 3 cut(s) 37, 79, 376
BspLI GGNNCC 2 cut(s) 51, 228
BspPI GGATC 2 cut(s) 87, 423
BspQI GCTCTTC 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 446
BssMI GATC 2 cut(s) 79, 415
BssNI GRCGYC 1 cut(s) 62
Bst4CI ACNGT 2 cut(s) 127, 159
Bst6I CTCTTC 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 167
BstDEI CTNAG 4 cut(s) 45, 57, 87, 384
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 2 cut(s) 82, 418
BstMBI GATC 2 cut(s) 79, 415
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 164
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 2 cut(s) 79, 415
BstYI RGATCY 2 cut(s) 79, 415
BsuI GTATCC 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 444
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 443
CseI GACGC 1 cut(s) 51
CviAII CATG 2 cut(s) 104, 221
CviJI RGCY 4 cut(s) 227, 262, 323, 360
CviKI_1 RGCY 4 cut(s) 227, 262, 323, 360
DdeI CTNAG 4 cut(s) 45, 57, 87, 384
DpnI GATC 2 cut(s) 81, 417
DpnII GATC 2 cut(s) 79, 415
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 167
FaeI CATG 2 cut(s) 107, 224
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 2 cut(s) 103, 220
FauNDI CATATG 1 cut(s) 232
GlaI GCGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 211
HaeII RGCGCY 1 cut(s) 103
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 102
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 107, 224
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 1 cut(s) 186
Hpy188I TCNGA 2 cut(s) 46, 84
Hpy188III TCNNGA 2 cut(s) 190, 419
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 3 cut(s) 70, 173, 329
HpyCH4III ACNGT 2 cut(s) 127, 159
HpyCH4IV ACGT 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 45, 57, 87, 384
HpySE526I ACGT 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 107, 224
HspAI GCGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 79, 415
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 97, 232
LpnPI CCDG 6 cut(s) 80, 98, 175, 321, 374, 459
MaeII ACGT 1 cut(s) 166
MalI GATC 2 cut(s) 81, 417
MboI GATC 2 cut(s) 79, 415
MboII GAAGA 5 cut(s) 195, 296, 366, 367, 434
MfeI CAATTG 1 cut(s) 278
MflI RGATCY 2 cut(s) 79, 415
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 8 cut(s) 28, 31, 40, 66, 135, 186, 244, 313
MseI TTAA 3 cut(s) 129, 146, 362
MunI CAATTG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 329
NdeI CATATG 1 cut(s) 232
NdeII GATC 2 cut(s) 79, 415
NlaIII CATG 2 cut(s) 107, 224
NlaIV GGNNCC 2 cut(s) 51, 228
PciSI GCTCTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 186
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 167
PspCI CACGTG 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 51, 228
PspPI GGNCC 1 cut(s) 443
PsuI RGATCY 2 cut(s) 79, 415
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 3 cut(s) 129, 146, 362
Sau3AI GATC 2 cut(s) 79, 415
Sau96I GGNCC 1 cut(s) 443
SduI GDGCHC 1 cut(s) 164
SetI ASST 9 cut(s) 42, 51, 146, 169, 264, 310, 362, 445, 460
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 1 cut(s) 278
SsiI CCGC 1 cut(s) 32
TaaI ACNGT 2 cut(s) 127, 159
TaiI ACGT 1 cut(s) 169
TaqI TCGA 2 cut(s) 65, 140
TasI AATT 1 cut(s) 278
TfiI GAWTC 1 cut(s) 186
Tru1I TTAA 3 cut(s) 129, 146, 362
Tru9I TTAA 3 cut(s) 129, 146, 362
TspDTI ATGAA 4 cut(s) 42, 168, 188, 366
VpaK11BI GGWCC 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.