Rw4G031090

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003016
Physical Location & Seq
Reverse (-)
57951928 .. 57954510
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031090.1

Sequence Viewer

Length: 900 bp
ATGGAAGACCTGTGGAAGCGAGCCAAGAGCTTCGCGGAAGAAGCCGCCAAGAAATCCCAGACAATCACCACCTCCTCCGCCTCCGCCAAAATCTCCGACTTAGTCTCCGAGACCGCCAAGCGATCCCGCGAGTTCGCCGCCGAGGCCTCCAAGAAAGCTGACGAGATCAAATCCGCCGCCCTCAAGCAAGCCGATCAGATCAAGTCTCTCTCCGTCTCCGAGATCATACCTCCTCAGCTCTCCAACCTCTCCCTCGTCAACTCCTCCTCCTCCTCCTCCTCCTCTGCCTCGGTCAATGAGCCTTCGCCGGAGGAGCTTCAGAAGTTTGGAGTCTCCGACGATCTCAGAGACTTCGTCAAGGGACTCACTTCCGATACCTTCAAGCACTTTCCGATCCAAGATGAAGCGGAGGAGGTTTCTGAGGTGCCGACTACGGCGTCGAATGTGAGGAAGGATCTGACTGAGTGGCAGGAGCGCCATGCCACTCTGGTTCTCACTACTGTTAAGGAGATTTCGAGGTTAAGATATGAACTGTGCCCACGTGTTATGAAAGAACGAAGATTCTGGAGGATATATTTTACCCTTGTGAGCAGTCATGTGGCTCCATATGAGAAGCAATATATGGAGGAGTTGAAGCTCAAGGAAGCAGAGCAATTGGAAGATGATAAAGCGAAGCAAACACCTGTTGTTGGAGAGGCTGATAAAGCAGAAGGAAGTGAAACCAACAAGAAGAGCTTAACTTCCAAATCTTCATCAACTGAGCAGGACTTGGATACATTCCTTTTGGGGGATCTTGAAGACAGCGATGGGGGTCAAGATGATGGCAACGACAATTTTGATGATGATTTCGACAAGATTGGCGATTCGGATGTTGATGATGAAAAGCAGGTGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

299

Amino Acids

33.37

Weight (kDa)

4.73

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 153 - 200 1.8e-11 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 877
AasI GACNNNNNNGTC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 877
AccB1I GGYRCC 1 cut(s) 424
AccII CGCG 2 cut(s) 35, 129
AclWI GGATC 4 cut(s) 117, 388, 462, 798
AcuI CTGAAG 1 cut(s) 302
AcvI CACGTG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 437
AfiI CCNNNNNNNGG 2 cut(s) 689, 787
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 3 cut(s) 382, 634, 797
AluBI AGCT 6 cut(s) 30, 158, 238, 316, 637, 735
AluI AGCT 6 cut(s) 30, 158, 238, 316, 637, 735
Alw26I GTCTC 6 cut(s) 104, 109, 210, 220, 337, 342
AlwI GGATC 4 cut(s) 117, 388, 462, 798
AoxI GGCC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 477
AsuHPI GGTGA 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 539
BanI GGYRCC 1 cut(s) 424
BbrPI CACGTG 1 cut(s) 542
BbsI GAAGAC 2 cut(s) 12, 804
BbvCI CCTCAGC 1 cut(s) 234
BccI CCATC 2 cut(s) 800, 815
BceAI ACGGC 1 cut(s) 450
BciVI GTATCC 1 cut(s) 766
BcoDI GTCTC 6 cut(s) 104, 109, 210, 220, 337, 342
BfoI RGCGCY 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 877
BfuI GTATCC 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 45, 138, 177
BlsI GCNGC 3 cut(s) 46, 139, 178
BmiI GGNNCC 2 cut(s) 426, 603
BpiI GAAGAC 2 cut(s) 12, 804
BpmI CTGGAG 1 cut(s) 586
Bpu10I CCTNAGC 1 cut(s) 234
BpuEI CTTGAG 2 cut(s) 167, 623
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 141, 288
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 689, 787
BseDI CCNNGG 2 cut(s) 141, 288
BseGI GGATG 1 cut(s) 874
BseLI CCNNNNNNNGG 2 cut(s) 689, 787
BseMII CTCAG 5 cut(s) 248, 358, 411, 453, 750
BseSI GKGCMC 1 cut(s) 539
Bsh1236I CGCG 2 cut(s) 35, 129
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 424
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 689, 787
BsmAI GTCTC 6 cut(s) 104, 109, 210, 220, 337, 342
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 5 cut(s) 247, 357, 412, 454, 751
BspFNI CGCG 2 cut(s) 35, 129
BspLI GGNNCC 2 cut(s) 426, 603
BspMI ACCTGC 1 cut(s) 877
BspPI GGATC 4 cut(s) 117, 388, 462, 798
BspQI GCTCTTC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 141, 288
BssMI GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
BssNI GRCGYC 1 cut(s) 437
Bst4CI ACNGT 2 cut(s) 502, 534
Bst6I CTCTTC 1 cut(s) 725
BstACI GRCGYC 1 cut(s) 437
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 21, 189
BstDEI CTNAG 6 cut(s) 100, 234, 344, 420, 462, 759
BstF5I GGATG 1 cut(s) 874
BstFNI CGCG 2 cut(s) 35, 129
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 9 cut(s) 125, 168, 196, 201, 225, 343, 396, 457, 793
BstMAI GTCTC 6 cut(s) 104, 109, 210, 220, 337, 342
BstMBI GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
BstMWI GCNNNNNNNGC 4 cut(s) 41, 143, 313, 704
BstSLI GKGCMC 1 cut(s) 539
BstUI CGCG 2 cut(s) 35, 129
BstV2I GAAGAC 2 cut(s) 12, 804
BstX2I RGATCY 2 cut(s) 454, 790
BstYI RGATCY 2 cut(s) 454, 790
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 1 cut(s) 874
BveI ACCTGC 1 cut(s) 877
Cac8I GCNNGC 2 cut(s) 21, 189
CfoI GCGC 1 cut(s) 477
CseI GACGC 1 cut(s) 426
CviAII CATG 2 cut(s) 479, 596
DdeI CTNAG 6 cut(s) 100, 234, 344, 420, 462, 759
DpnI GATC 9 cut(s) 124, 167, 195, 200, 224, 342, 395, 456, 792
DpnII GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
DrdI GACNNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 725
EarI CTCTTC 1 cut(s) 725
EciI GGCGGA 3 cut(s) 67, 73, 163
Eco147I AGGCCT 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 302
Eco72I CACGTG 1 cut(s) 542
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 2 cut(s) 482, 599
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 1 cut(s) 375
FatI CATG 2 cut(s) 478, 595
FauI CCCGC 1 cut(s) 134
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 45, 138, 177
FokI GGATG 1 cut(s) 881
Fsp4HI GCNGC 3 cut(s) 45, 138, 177
GlaI GCGC 1 cut(s) 476
GluI GCNGC 3 cut(s) 45, 138, 177
GsuI CTGGAG 1 cut(s) 586
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 426
HhaI GCGC 1 cut(s) 477
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 2 cut(s) 482, 599
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 330, 363, 561, 863
HpaII CCGG 1 cut(s) 308
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 259
Hpy188III TCNNGA 3 cut(s) 565, 794, 815
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 2 cut(s) 341, 442
HpyAV CCTTC 4 cut(s) 312, 388, 445, 704
HpyCH4III ACNGT 2 cut(s) 502, 534
HpyCH4IV ACGT 1 cut(s) 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 143, 313, 704
HpyF3I CTNAG 6 cut(s) 100, 234, 344, 420, 462, 759
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 2 cut(s) 482, 599
HspAI GCGC 1 cut(s) 475
Kzo9I GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
LguI GCTCTTC 1 cut(s) 725
LmnI GCTCC 3 cut(s) 313, 472, 607
LpnPI CCDG 9 cut(s) 23, 71, 321, 455, 473, 550, 696, 749, 872
MaeII ACGT 1 cut(s) 541
MalI GATC 9 cut(s) 124, 167, 195, 200, 224, 342, 395, 456, 792
MboI GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
MboII GAAGA 7 cut(s) 17, 50, 570, 671, 741, 742, 809
MfeI CAATTG 1 cut(s) 653
MflI RGATCY 2 cut(s) 454, 790
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 2 cut(s) 653, 832
MlyI GAGTC 2 cut(s) 339, 357
MmeI TCCRAC 4 cut(s) 120, 267, 360, 670
MseI TTAA 3 cut(s) 504, 521, 737
MspI CCGG 1 cut(s) 308
MunI CAATTG 1 cut(s) 653
MvnI CGCG 2 cut(s) 35, 129
MwoI GCNNNNNNNGC 4 cut(s) 41, 143, 313, 704
NdeI CATATG 1 cut(s) 607
NdeII GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
NlaIII CATG 2 cut(s) 482, 599
NlaIV GGNNCC 2 cut(s) 426, 603
NmeAIII GCCGAG 1 cut(s) 166
PaqCI CACCTGC 1 cut(s) 877
PceI AGGCCT 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 725
PfeI GAWTC 2 cut(s) 561, 863
PflFI GACNNNGTC 2 cut(s) 101, 353
PkrI GCNGC 3 cut(s) 46, 139, 178
PleI GAGTC 2 cut(s) 338, 357
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
PpsI GAGTC 2 cut(s) 338, 357
Ppu21I YACGTR 1 cut(s) 542
PspCI CACGTG 1 cut(s) 542
PspN4I GGNNCC 2 cut(s) 426, 603
PsuI RGATCY 2 cut(s) 454, 790
PsyI GACNNNGTC 2 cut(s) 101, 353
SapI GCTCTTC 1 cut(s) 725
SaqAI TTAA 3 cut(s) 504, 521, 737
SatI GCNGC 3 cut(s) 45, 138, 177
Sau3AI GATC 9 cut(s) 122, 165, 193, 198, 222, 340, 393, 454, 790
SchI GAGTC 2 cut(s) 339, 357
SduI GDGCHC 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 182, 638
SmoI CTYRAG 2 cut(s) 182, 638
Sse9I AATT 2 cut(s) 653, 832
SseBI AGGCCT 1 cut(s) 146
StuI AGGCCT 1 cut(s) 146
TaaI ACNGT 2 cut(s) 502, 534
TaiI ACGT 1 cut(s) 544
TaqI TCGA 3 cut(s) 440, 515, 849
TaqII GACCGA 1 cut(s) 280
TasI AATT 2 cut(s) 653, 832
TauI GCSGC 3 cut(s) 47, 140, 179
TfiI GAWTC 2 cut(s) 561, 863
Tru1I TTAA 3 cut(s) 504, 521, 737
Tru9I TTAA 3 cut(s) 504, 521, 737
TspDTI ATGAA 5 cut(s) 417, 543, 563, 741, 894
TspGWI ACGGA 1 cut(s) 202
Tth111I GACNNNGTC 2 cut(s) 101, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.