MD17G1002300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002629
Physical Location & Seq
Reverse (-)
155616 .. 158460
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1002300.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGAAGTTATTACAGTGGGTGCACCACAAATTTCGGCACGGTAGCATTGAGCCTTTCAAGGATCTCACTATTGCTCAGCCATCACTTGATGACCAAGATGCCTATATGAAGTCAAGCTTTGGCTCCAGATACAGATCTACATCCTTGGAGCCACCTGCAAGGGACCGAGAAAAATCTTTTTCTGAATCTGAAGCCAAGAGAGAAGAAGAAACATCTGCTATCATCTCAGAGCTTTTTCCTGGCTTTCTCACCATTGGAACTCTTGGCTCAGAGTCAGGTATCAATGAGCCCGAAACACCAACTTTTGGGACGACCTTAGAGAACTTAACTCAGCAAAAAGCAGAGGTGACTGAGAACGACTTGAAGCTGATCAGATACGAGCTAGAGAAGTTTCTTGATGCTGAGACAAAGGAAGAAGGGGTTCGTGTATCGTCAGCAAGGGACAGTCATGCTAGCTCTGTTACACCCAATGGAAAGCAAATGGAGGAGTCCGAAGATGAAGAGTACTGGAAGGATGCAGCATGTCCGCTCAAAGGATATCTCTTTGGGTCCTCAACTGAATTACCAGAAACAATGGAAGCAAAGAAAGAAAAGGCATCACTGCAAGAGCTGTTTGACAGGACCAAAATCACAACTGATTATAAGGAGAAAAGTGAAAGTGAGGAGACAGAAGTCAAGTATAAGCATAAATCTGCCATGGGGTTCATGAAGAAGATGATTAAAAAGTTCCATGCTTCTTCAAAGAGCTCAGGTGGTGATGCAACTGATTCTGTTTCCATCAAGAAGAAATGTGGTGAGGCATCTGATTCTCTTTCAACCAAGAAGAAACCCCATAAGGTCTTACGCATGTTCCATAGAACGATCCATCCTGAAAGCTCTATTGCTGCTAGAGAAGTTGTCAAGTCTGAGAAATACAAGAAGAAGAAAATTTTCAGTGCAGATAGTGGTTGTAATGAAAACATGATGCTCAAGGGTGGAGACAACAGAAGGTTCCCTGAAGGGCCACTGGTAAAGGAGGGGACTGAAAATTGTAAGAAATACATGAACTTCCCCCAGTACAGGCTGAATGGCAGCACTTACGGGACAAGAGCAGAGCACTGGATTAAAACAGATGCAGAATATTTGGTGTTGGAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009958 GO:0009959 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

43.02

Weight (kDa)

6.6

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 1 cut(s) 163
AccB7I CCANNNNNTGG 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 529
AciI CCGC 1 cut(s) 527
AclWI GGATC 2 cut(s) 69, 856
AcsI RAATTY 2 cut(s) 29, 927
AcuI CTGAAG 2 cut(s) 210, 1017
AfaI GTAC 2 cut(s) 506, 1058
AfiI CCNNNNNNNGG 3 cut(s) 305, 533, 1059
AgsI TTSAA 4 cut(s) 58, 364, 741, 816
AjnI CCWGG 1 cut(s) 238
AloI GAACNNNNNNTCC 2 cut(s) 850, 882
AluBI AGCT 9 cut(s) 117, 232, 367, 382, 456, 610, 747, 876, 1135
AluI AGCT 9 cut(s) 117, 232, 367, 382, 456, 610, 747, 876, 1135
Alw21I GWGCWC 3 cut(s) 24, 749, 1098
Alw26I GTCTC 3 cut(s) 398, 659, 972
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 2 cut(s) 69, 856
AoxI GGCC 1 cut(s) 1001
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 518, 884, 1071
ApoI RAATTY 2 cut(s) 29, 927
Asp700I GAANNNNTTC 2 cut(s) 420, 929
AspS9I GGNCC 4 cut(s) 163, 549, 621, 1001
AsuHPI GGTGA 4 cut(s) 241, 358, 767, 806
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 3 cut(s) 163, 549, 621
BaeGI GKGCMC 1 cut(s) 24
BanII GRGCYC 2 cut(s) 291, 749
Bbv12I GWGCWC 3 cut(s) 24, 749, 1098
BbvI GCAGC 3 cut(s) 530, 871, 1083
BccI CCATC 3 cut(s) 88, 785, 873
BciT130I CCWGG 1 cut(s) 240
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 3 cut(s) 398, 659, 972
BfaI CTAG 3 cut(s) 383, 453, 888
BfmI CTRYAG 1 cut(s) 1136
BfuAI ACCTGC 1 cut(s) 163
BglII AGATCT 1 cut(s) 134
BisI GCNGC 3 cut(s) 519, 885, 1072
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 3 cut(s) 520, 886, 1073
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 1 cut(s) 240
Bme18I GGWCC 3 cut(s) 163, 549, 621
BmgT120I GGNCC 4 cut(s) 163, 549, 621, 1001
BmiI GGNNCC 5 cut(s) 124, 150, 164, 550, 992
BmrFI CCNGG 1 cut(s) 240
BmrI ACTGGG 1 cut(s) 1048
BmsI GCATC 8 cut(s) 88, 388, 505, 605, 748, 809, 954, 1102
BmtI GCTAGC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 1048
BoxI GACNNNNGTC 1 cut(s) 671
BpmI CTGGAG 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 748
Bpu1102I GCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 953
BsaBI GATNNNNATC 2 cut(s) 133, 139
BsaJI CCNNGG 2 cut(s) 144, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 533, 1059
Bse1I ACTGG 4 cut(s) 512, 1011, 1054, 1103
Bse8I GATNNNNATC 2 cut(s) 133, 139
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 144, 696
BseGI GGATG 3 cut(s) 140, 520, 865
BseJI GATNNNNATC 2 cut(s) 133, 139
BseLI CCNNNNNNNGG 3 cut(s) 305, 533, 1059
BseMII CTCAG 8 cut(s) 89, 240, 282, 342, 344, 393, 762, 897
BseNI ACTGG 4 cut(s) 512, 1011, 1054, 1103
BseRI GAGGAG 2 cut(s) 500, 677
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 3 cut(s) 530, 871, 1083
BsgI GTGCAG 1 cut(s) 957
BshFI GGCC 1 cut(s) 1003
BsiHKAI GWGCWC 3 cut(s) 24, 749, 1098
BslFI GGGAC 5 cut(s) 176, 322, 455, 1033, 1096
BslI CCNNNNNNNGG 3 cut(s) 305, 533, 1059
BsmAI GTCTC 3 cut(s) 398, 659, 972
BsmFI GGGAC 5 cut(s) 176, 322, 455, 1033, 1096
BsnI GGCC 1 cut(s) 1003
Bsp1286I GDGCHC 4 cut(s) 24, 291, 749, 1098
Bsp143I GATC 4 cut(s) 61, 134, 369, 861
Bsp1720I GCTNAGC 1 cut(s) 75
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 1 cut(s) 527
BspANI GGCC 1 cut(s) 1003
BspCNI CTCAG 8 cut(s) 88, 239, 281, 343, 343, 394, 761, 898
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 5 cut(s) 124, 150, 164, 550, 992
BspMI ACCTGC 1 cut(s) 163
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 69, 856
BsrBI CCGCTC 1 cut(s) 529
BsrI ACTGG 4 cut(s) 512, 1011, 1054, 1103
BssECI CCNNGG 2 cut(s) 144, 696
BssMI GATC 4 cut(s) 61, 134, 369, 861
BssT1I CCWWGG 2 cut(s) 144, 696
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 3 cut(s) 15, 41, 446
Bst6I CTCTTC 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 9 cut(s) 75, 226, 268, 316, 330, 351, 402, 748, 906
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 3 cut(s) 140, 520, 865
BstKTI GATC 4 cut(s) 64, 137, 372, 864
BstMAI GTCTC 3 cut(s) 398, 659, 972
BstMBI GATC 4 cut(s) 61, 134, 369, 861
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 2 cut(s) 525, 850
BstPAI GACNNNNGTC 1 cut(s) 671
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 1136
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 530, 871, 1083
BstX2I RGATCY 2 cut(s) 61, 134
BstYI RGATCY 2 cut(s) 61, 134
BsuRI GGCC 1 cut(s) 1003
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 3 cut(s) 140, 520, 865
BtsI GCAGTG 1 cut(s) 599
BtsIMutI CAGTG 5 cut(s) 20, 599, 940, 1004, 1096
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 454
CciI TCATGA 1 cut(s) 705
Cfr13I GGNCC 4 cut(s) 163, 549, 621, 1001
Csp6I GTAC 2 cut(s) 505, 1057
CviAII CATG 8 cut(s) 449, 522, 697, 706, 731, 847, 961, 1042
CviQI GTAC 2 cut(s) 505, 1057
DdeI CTNAG 9 cut(s) 75, 226, 268, 316, 330, 351, 402, 748, 906
DpnI GATC 4 cut(s) 63, 136, 371, 863
DpnII GATC 4 cut(s) 61, 134, 369, 861
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 747
Eco130I CCWWGG 2 cut(s) 144, 696
Eco24I GRGCYC 2 cut(s) 291, 749
Eco32I GATATC 1 cut(s) 539
Eco47I GGWCC 3 cut(s) 163, 549, 621
Eco53kI GAGCTC 1 cut(s) 747
Eco57I CTGAAG 2 cut(s) 210, 1017
EcoICRI GAGCTC 1 cut(s) 747
EcoO109I RGGNCCY 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 539
EcoT14I CCWWGG 2 cut(s) 144, 696
EcoT38I GRGCYC 2 cut(s) 291, 749
ErhI CCWWGG 2 cut(s) 144, 696
FaeI CATG 8 cut(s) 452, 525, 700, 709, 734, 850, 964, 1045
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 5 cut(s) 176, 322, 455, 1033, 1096
FatI CATG 8 cut(s) 448, 521, 696, 705, 730, 846, 960, 1041
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 519, 885, 1072
FokI GGATG 3 cut(s) 127, 527, 852
FriOI GRGCYC 2 cut(s) 291, 749
Fsp4HI GCNGC 3 cut(s) 519, 885, 1072
FspBI CTAG 3 cut(s) 383, 453, 888
GluI GCNGC 3 cut(s) 519, 885, 1072
GsuI CTGGAG 1 cut(s) 109
HaeIII GGCC 1 cut(s) 1003
Hin1II CATG 8 cut(s) 452, 525, 700, 709, 734, 850, 964, 1045
HindIII AAGCTT 1 cut(s) 115
HinfI GANTC 5 cut(s) 185, 272, 488, 767, 806
HphI GGTGA 4 cut(s) 241, 358, 767, 806
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 8 cut(s) 184, 190, 229, 271, 374, 493, 805, 907
Hpy188III TCNNGA 5 cut(s) 126, 395, 706, 781, 869
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 4 cut(s) 410, 505, 981, 992
HpyCH4III ACNGT 3 cut(s) 15, 41, 446
HpyCH4V TGCA 7 cut(s) 22, 158, 518, 604, 761, 938, 1115
HpyF3I CTNAG 9 cut(s) 75, 226, 268, 316, 330, 351, 402, 748, 906
Hsp92II CATG 8 cut(s) 452, 525, 700, 709, 734, 850, 964, 1045
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 4 cut(s) 61, 134, 369, 861
LmnI GCTCC 3 cut(s) 128, 148, 1132
Lsp1109I GCAGC 3 cut(s) 530, 871, 1083
LweI GCATC 8 cut(s) 88, 388, 505, 605, 748, 809, 954, 1102
MaeI CTAG 3 cut(s) 383, 453, 888
MaeIII GTNAC 2 cut(s) 346, 460
MalI GATC 4 cut(s) 63, 136, 371, 863
MbiI CCGCTC 1 cut(s) 529
MboI GATC 4 cut(s) 61, 134, 369, 861
MflI RGATCY 2 cut(s) 61, 134
MhlI GDGCHC 4 cut(s) 24, 291, 749, 1098
MluCI AATT 4 cut(s) 29, 560, 927, 1027
MlyI GAGTC 2 cut(s) 281, 497
MmeI TCCRAC 1 cut(s) 1110
MnlI CCTC 6 cut(s) 337, 478, 562, 655, 790, 1009
MroXI GAANNNNTTC 2 cut(s) 420, 929
MseI TTAA 3 cut(s) 326, 720, 1104
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 696
NdeII GATC 4 cut(s) 61, 134, 369, 861
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 8 cut(s) 452, 525, 700, 709, 734, 850, 964, 1045
NlaIV GGNNCC 5 cut(s) 124, 150, 164, 550, 992
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 2 cut(s) 525, 850
PagI TCATGA 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 420, 929
PfeI GAWTC 3 cut(s) 185, 767, 806
PflMI CCANNNNNTGG 1 cut(s) 305
PkrI GCNGC 3 cut(s) 520, 886, 1073
PleI GAGTC 2 cut(s) 280, 496
PpsI GAGTC 2 cut(s) 280, 496
PpuMI RGGWCCY 1 cut(s) 549
PshAI GACNNNNGTC 1 cut(s) 671
PsiI TTATAA 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 5 cut(s) 124, 150, 164, 550, 992
PspPI GGNCC 4 cut(s) 163, 549, 621, 1001
PspPPI RGGWCCY 1 cut(s) 549
PsuI RGATCY 2 cut(s) 61, 134
RsaI GTAC 2 cut(s) 506, 1058
RsaNI GTAC 2 cut(s) 505, 1057
SacI GAGCTC 1 cut(s) 749
SaqAI TTAA 3 cut(s) 326, 720, 1104
SatI GCNGC 3 cut(s) 519, 885, 1072
Sau3AI GATC 4 cut(s) 61, 134, 369, 861
Sau96I GGNCC 4 cut(s) 163, 549, 621, 1001
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 2 cut(s) 281, 497
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 4 cut(s) 24, 291, 749, 1098
SfaNI GCATC 8 cut(s) 88, 388, 505, 605, 748, 809, 954, 1102
SfcI CTRYAG 1 cut(s) 1136
SinI GGWCC 3 cut(s) 163, 549, 621
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
Sse9I AATT 4 cut(s) 29, 560, 927, 1027
SsiI CCGC 1 cut(s) 527
SspI AATATT 1 cut(s) 1121
SspMI CTAG 3 cut(s) 383, 453, 888
SstI GAGCTC 1 cut(s) 749
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 2 cut(s) 144, 696
TaaI ACNGT 3 cut(s) 15, 41, 446
TaqII GACCGA 1 cut(s) 180
TasI AATT 4 cut(s) 29, 560, 927, 1027
TatI WGTACW 2 cut(s) 504, 1056
TfiI GAWTC 3 cut(s) 185, 767, 806
Tru1I TTAA 3 cut(s) 326, 720, 1104
Tru9I TTAA 3 cut(s) 326, 720, 1104
TscAI CASTG 5 cut(s) 20, 606, 940, 1011, 1103
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 3 cut(s) 518, 884, 1071
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 7 cut(s) 17, 122, 513, 694, 722, 969, 1058
TspRI CASTG 5 cut(s) 20, 606, 940, 1011, 1103
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 3 cut(s) 163, 549, 621
XapI RAATTY 2 cut(s) 29, 927
XceI RCATGY 2 cut(s) 525, 850
XmnI GAANNNNTTC 2 cut(s) 420, 929
XspI CTAG 3 cut(s) 383, 453, 888
ZrmI AGTACT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.