pycom16g10510

regulation of auxin polar transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002629
Physical Location & Seq
Reverse (-)
7172700 .. 7176422
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g10510.3

Sequence Viewer

Length: 1143 bp
ATGAAGTTACTAGGTTGGATGCATCGCATGTTTCGGCAGAATAGCAACGATCCGTTTCAAGTTTCCGTCGTTGGACAGCCATCGCTCGACGATCAACAATACTATCAGAAGTCAAACTGGGGCACGAAACCCTTAACGCAAGCCCCGAAAGATCAGCACCTTCGAAAATCTTTCAACAGTCTAGAAGCAGCCAGGACAGAAGAAGAATACTATGAAGAGGAATCATCTGCTGAGGCATCTGAGCTCTTCCATGGCTTTCTTGCAATCGGCACCCTTGGAAGCTTGGACCAAGTGAACACTGAACCATCAACCCCAATGCTTGGAATCTCCGTGGAGAACATAACCGAAAAAGAAACTGAGGCCGCAGAGAACGAGTTGAATCTCATCAATGACGAATTGGAGAAAGAGCTAGTGGCAGATCCGGCGAAGGATGATATTTGCAATGATTTATCTGGAAGAAACAGCTATGTCAGCAATGGAAGAAGTAGCCAAGGAAGCACCATTACGCTAAGCGGGAAGGCACTGGAAGGCGCAGAGAACAATGGGAATAATGGAACGGCAGTGTGCCCGCTCCAGGGATATCTTTTCGGGTCGGGATATGTGTTGTCTGAAACAATAATAGTGGCAAAGAAGGAACACAGGACATCTCTTGGTGAGCTGTTCCAGAGGACTAAATTGGCTGAGGAGATCTCCGGAGCAGAATTGATCAAAGAGGAGAAGCAAGCGGATAAGTCCGCCATGCACTTAATCAAAAAGATGCTCAAGAAAAAAATGCTTCACGCTTCTTCTCGTGGCTCCGGCAGAGCTGCTGATCATGCTTCAGCAGAAACAAAACTCAATAAGATCCTTCACATGTTCCGTAGAAAAGTTCACCCTGAAACCTCGGCGGCTGAGCGAAAACCCGGTAAGTACAAAGGGAACCAAAGCAGGAAGAAACCAAGCAGTGAGGGGACTTACAACAATGGAGATCAGGATGAAGACATCTTGTTATATCCTCAACAAGGATTTTCGTCAAATCAGAGCATGCGGCACTACAAGAGCCAATCAAACCCACCCCAATTTGTGCTTAGCGGCATGAATTCAAATGAGAACAGGGGGCACTGGATCAAAACAGGTGCAGACTACCTAATCCTGGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0008150 GO:0010817 GO:0016020 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0051049 GO:0065007 GO:0065008 GO:0071944 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

42.32

Weight (kDa)

6.05

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 320
AccBSI CCGCTC 1 cut(s) 571
AccIII TCCGGA 1 cut(s) 692
AciI CCGC 8 cut(s) 363, 513, 569, 725, 735, 887, 1027, 1071
AclWI GGATC 4 cut(s) 44, 413, 838, 1112
AcsI RAATTY 1 cut(s) 1078
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 1 cut(s) 911
AfiI CCNNNNNNNGG 5 cut(s) 320, 574, 575, 1001, 1132
AflIII ACRYGT 1 cut(s) 852
AgsI TTSAA 4 cut(s) 59, 175, 379, 1083
AjnI CCWGG 3 cut(s) 191, 573, 1131
AluBI AGCT 7 cut(s) 244, 282, 409, 465, 658, 806, 1138
AluI AGCT 7 cut(s) 244, 282, 409, 465, 658, 806, 1138
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 4 cut(s) 44, 413, 838, 1112
Aor13HI TCCGGA 1 cut(s) 692
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 2 cut(s) 188, 806
ApoI RAATTY 1 cut(s) 1078
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 2 cut(s) 665, 863
AsuII TTCGAA 1 cut(s) 163
AvaII GGWCC 1 cut(s) 286
BaeGI GKGCMC 3 cut(s) 125, 569, 1101
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 487, 519
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 1 cut(s) 984
Bbv12I GWGCWC 1 cut(s) 246
BbvCI CCTCAGC 2 cut(s) 231, 681
BbvI GCAGC 2 cut(s) 200, 793
BccI CCATC 2 cut(s) 88, 313
BceAI ACGGC 1 cut(s) 573
BciT130I CCWGG 3 cut(s) 193, 575, 1133
BclI TGATCA 2 cut(s) 705, 811
BcnI CCSGG 1 cut(s) 903
BfaI CTAG 3 cut(s) 11, 182, 410
BglII AGATCT 1 cut(s) 687
BisI GCNGC 6 cut(s) 189, 363, 807, 888, 1028, 1072
BlpI GCTNAGC 3 cut(s) 509, 891, 1067
BlsI GCNGC 6 cut(s) 190, 364, 808, 889, 1029, 1073
Bme1390I CCNGG 4 cut(s) 193, 575, 903, 1133
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 271, 796, 920
BmrFI CCNGG 4 cut(s) 193, 575, 903, 1133
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 4 cut(s) 9, 31, 245, 747
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 984
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 557
Bpu10I CCTNAGC 2 cut(s) 231, 681
Bpu1102I GCTNAGC 3 cut(s) 509, 891, 1067
Bpu14I TTCGAA 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 903
BsaJI CCNNGG 6 cut(s) 250, 274, 330, 490, 574, 882
BsaWI WCCGGW 1 cut(s) 692
Bsc4I CCNNNNNNNGG 5 cut(s) 320, 574, 575, 1001, 1132
Bse1I ACTGG 3 cut(s) 122, 528, 1106
Bse3DI GCAATG 2 cut(s) 448, 481
BseAI TCCGGA 1 cut(s) 692
BseBI CCWGG 3 cut(s) 193, 575, 1133
BseDI CCNNGG 6 cut(s) 250, 274, 330, 490, 574, 882
BseGI GGATG 3 cut(s) 24, 436, 979
BseLI CCNNNNNNNGG 5 cut(s) 320, 574, 575, 1001, 1132
BseMI GCAATG 2 cut(s) 448, 481
BseMII CTCAG 5 cut(s) 222, 231, 348, 672, 882
BseNI ACTGG 3 cut(s) 122, 528, 1106
BseRI GAGGAG 2 cut(s) 698, 728
BseSI GKGCMC 3 cut(s) 125, 569, 1101
BseXI GCAGC 2 cut(s) 200, 793
BsgI GTGCAG 1 cut(s) 1137
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 4 cut(s) 422, 693, 798, 903
BslFI GGGAC 1 cut(s) 964
BslI CCNNNNNNNGG 5 cut(s) 320, 574, 575, 1001, 1132
BsmFI GGGAC 1 cut(s) 964
BsnI GGCC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 163
Bsp1286I GDGCHC 4 cut(s) 125, 246, 569, 1101
Bsp13I TCCGGA 1 cut(s) 692
Bsp1720I GCTNAGC 3 cut(s) 509, 891, 1067
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 8 cut(s) 363, 513, 569, 725, 735, 887, 1027, 1071
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 5 cut(s) 223, 232, 349, 673, 883
BspEI TCCGGA 1 cut(s) 692
BspLI GGNNCC 3 cut(s) 271, 796, 920
BspPI GGATC 4 cut(s) 44, 413, 838, 1112
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 571
BsrDI GCAATG 2 cut(s) 448, 481
BsrI ACTGG 3 cut(s) 122, 528, 1106
BssECI CCNNGG 6 cut(s) 250, 274, 330, 490, 574, 882
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 3 cut(s) 250, 274, 490
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 3 cut(s) 193, 575, 1133
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 2 cut(s) 210, 251
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 4 cut(s) 141, 569, 723, 1025
BstDEI CTNAG 7 cut(s) 231, 240, 357, 509, 681, 891, 1067
BstDSI CCRYGG 2 cut(s) 250, 330
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 3 cut(s) 24, 436, 979
BstHHI GCGC 1 cut(s) 533
BstMWI GCNNNNNNNGC 4 cut(s) 422, 471, 495, 815
BstNI CCWGG 3 cut(s) 193, 575, 1133
BstNSI RCATGY 3 cut(s) 31, 856, 1027
BstSCI CCNGG 4 cut(s) 191, 573, 901, 1131
BstSLI GKGCMC 3 cut(s) 125, 569, 1101
BstV1I GCAGC 2 cut(s) 200, 793
BstV2I GAAGAC 1 cut(s) 984
BstX2I RGATCY 3 cut(s) 418, 687, 843
BstYI RGATCY 3 cut(s) 418, 687, 843
BsuRI GGCC 1 cut(s) 362
BtgI CCRYGG 2 cut(s) 250, 330
BtgZI GCGATG 2 cut(s) 8, 66
BtsCI GGATG 3 cut(s) 24, 436, 979
BtsI GCAGTG 2 cut(s) 567, 949
BtsIMutI CAGTG 5 cut(s) 297, 521, 567, 949, 1099
Cac8I GCNNGC 4 cut(s) 141, 569, 723, 1025
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 910
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviAII CATG 7 cut(s) 28, 251, 739, 815, 853, 1024, 1075
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 7 cut(s) 231, 240, 357, 509, 681, 891, 1067
Eam1104I CTCTTC 2 cut(s) 210, 251
EarI CTCTTC 2 cut(s) 210, 251
EciI GGCGGA 1 cut(s) 724
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 3 cut(s) 250, 274, 490
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 3 cut(s) 191, 573, 1131
EcoRV GATATC 1 cut(s) 581
EcoT14I CCWWGG 3 cut(s) 250, 274, 490
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 3 cut(s) 250, 274, 490
FaeI CATG 7 cut(s) 31, 254, 742, 818, 856, 1027, 1078
FaqI GGGAC 1 cut(s) 964
FatI CATG 7 cut(s) 27, 250, 738, 814, 852, 1023, 1074
FauI CCCGC 2 cut(s) 506, 576
FbaI TGATCA 2 cut(s) 705, 811
Fnu4HI GCNGC 6 cut(s) 189, 363, 807, 888, 1028, 1072
FokI GGATG 3 cut(s) 31, 443, 986
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 6 cut(s) 189, 363, 807, 888, 1028, 1072
FspBI CTAG 3 cut(s) 11, 182, 410
GlaI GCGC 1 cut(s) 532
GluI GCNGC 6 cut(s) 189, 363, 807, 888, 1028, 1072
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 1 cut(s) 362
HapII CCGG 4 cut(s) 422, 693, 798, 903
HhaI GCGC 1 cut(s) 533
Hin1II CATG 7 cut(s) 31, 254, 742, 818, 856, 1027, 1078
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 3 cut(s) 221, 324, 379
HpaII CCGG 4 cut(s) 422, 693, 798, 903
HphI GGTGA 2 cut(s) 665, 863
Hpy166II GTNNAC 2 cut(s) 295, 871
Hpy188I TCNGA 4 cut(s) 108, 241, 610, 1020
Hpy188III TCNNGA 7 cut(s) 182, 453, 594, 664, 693, 763, 971
Hpy8I GTNNAC 2 cut(s) 295, 871
Hpy99I CGWCG 2 cut(s) 71, 92
HpyAV CCTTC 6 cut(s) 170, 421, 511, 521, 625, 857
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 22, 263, 441, 742, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 422, 471, 495, 815
HpyF3I CTNAG 7 cut(s) 231, 240, 357, 509, 681, 891, 1067
Hsp92II CATG 7 cut(s) 31, 254, 742, 818, 856, 1027, 1078
HspAI GCGC 1 cut(s) 531
Kpn2I TCCGGA 1 cut(s) 692
Ksp22I TGATCA 2 cut(s) 705, 811
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 4 cut(s) 576, 695, 800, 1135
Lsp1109I GCAGC 2 cut(s) 200, 793
LweI GCATC 4 cut(s) 9, 31, 245, 747
MaeI CTAG 3 cut(s) 11, 182, 410
MaeIII GTNAC 1 cut(s) 6
MbiI CCGCTC 1 cut(s) 571
MboII GAAGA 9 cut(s) 212, 215, 227, 238, 468, 492, 777, 943, 989
MflI RGATCY 3 cut(s) 418, 687, 843
MhlI GDGCHC 4 cut(s) 125, 246, 569, 1101
MluCI AATT 5 cut(s) 395, 674, 701, 1058, 1078
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 9 cut(s) 211, 226, 352, 660, 676, 706, 892, 940, 1005
Mph1103I ATGCAT 1 cut(s) 24
MroI TCCGGA 1 cut(s) 692
MseI TTAA 2 cut(s) 134, 746
MspI CCGG 4 cut(s) 422, 693, 798, 903
MspR9I CCNGG 4 cut(s) 193, 575, 903, 1133
MvaI CCWGG 3 cut(s) 193, 575, 1133
MwoI GCNNNNNNNGC 4 cut(s) 422, 471, 495, 815
NciI CCSGG 1 cut(s) 903
NcoI CCATGG 1 cut(s) 250
NlaIII CATG 7 cut(s) 31, 254, 742, 818, 856, 1027, 1078
NlaIV GGNNCC 3 cut(s) 271, 796, 920
NmeAIII GCCGAG 1 cut(s) 863
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 3 cut(s) 31, 856, 1027
NspV TTCGAA 1 cut(s) 163
PaeI GCATGC 1 cut(s) 1027
PciI ACATGT 1 cut(s) 852
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PfeI GAWTC 3 cut(s) 221, 324, 379
PflMI CCANNNNNTGG 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 1131
PkrI GCNGC 6 cut(s) 190, 364, 808, 889, 1029, 1073
PscI ACATGT 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 3 cut(s) 191, 573, 1131
PspGI CCWGG 3 cut(s) 191, 573, 1131
PspN4I GGNNCC 3 cut(s) 271, 796, 920
PspPI GGNCC 1 cut(s) 286
PsuI RGATCY 3 cut(s) 418, 687, 843
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 2 cut(s) 134, 746
SatI GCNGC 6 cut(s) 189, 363, 807, 888, 1028, 1072
Sau96I GGNCC 1 cut(s) 286
ScrFI CCNGG 4 cut(s) 193, 575, 903, 1133
SduI GDGCHC 4 cut(s) 125, 246, 569, 1101
SfaNI GCATC 4 cut(s) 9, 31, 245, 747
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SphI GCATGC 1 cut(s) 1027
Sse9I AATT 5 cut(s) 395, 674, 701, 1058, 1078
SsiI CCGC 8 cut(s) 363, 513, 569, 725, 735, 887, 1027, 1071
SspMI CTAG 3 cut(s) 11, 182, 410
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 4 cut(s) 191, 573, 901, 1131
StyI CCWWGG 3 cut(s) 250, 274, 490
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 87, 163
TasI AATT 5 cut(s) 395, 674, 701, 1058, 1078
TatI WGTACW 1 cut(s) 909
TauI GCSGC 4 cut(s) 365, 890, 1030, 1074
TfiI GAWTC 3 cut(s) 221, 324, 379
Tru1I TTAA 2 cut(s) 134, 746
Tru9I TTAA 2 cut(s) 134, 746
TscAI CASTG 5 cut(s) 304, 528, 567, 949, 1106
TseI GCWGC 2 cut(s) 188, 806
TspDTI ATGAA 4 cut(s) 17, 228, 990, 1091
TspGWI ACGGA 4 cut(s) 42, 55, 319, 848
TspRI CASTG 5 cut(s) 304, 528, 567, 949, 1106
Van91I CCANNNNNTGG 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 286
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 1 cut(s) 1078
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 3 cut(s) 31, 856, 1027
XspI CTAG 3 cut(s) 11, 182, 410
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.