Prupe.8G137400_v2.0.a1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001279
Physical Location & Seq
Forward (+)
15696755 .. 15699366
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G137400.3

Sequence Viewer

Length: 432 bp
ATGGCTAAGAATTCAACGGTCAAGATGATTGATGCACATCAGCTGTCGTGCACCTGTGCAGCACCATCCCCCACAAAACAAAACCCTCACAAAGCCCTCGCCAGAGAAGCCACACTCTTGGCAACAGTAACTCAGAAAATGGCTTGGCGCTCAGCTGGTTCGTTGTCTCGGTCATTGGTTTCTACGGCTAGGGTTTCGTCGCTCCGTTCGGCGCCACCTCTCCGTCGTCTCCGTCCACCGACTGTCTCCACTCCTCGTCGCCTCGCATTCGCTTCTCCCAGGAACTTGGGAGAACTGGGCTGCATACAATCGCTTTTGCCAATGCACAGTGTGACGGCTGCAACTTGCCTCACATCTCATCTGGCTGTCAATGCGCGTGGTTGTTGCGAGTTGTCACACGGTACCTTCTGCCGTACTTGTCAGGATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

15.35

Weight (kDa)

10.79

Isoelectric Point (pI)

67.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 2 cut(s) 211, 401
AccII CGCG 1 cut(s) 376
AcsI RAATTY 1 cut(s) 10
AcyI GRCGYC 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 403, 415
AgsI TTSAA 1 cut(s) 15
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 2 cut(s) 43, 155
AluI AGCT 2 cut(s) 43, 155
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 3 cut(s) 171, 233, 250
Alw44I GTGCAC 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 49
ApeKI GCWGC 3 cut(s) 59, 300, 338
ApoI RAATTY 1 cut(s) 10
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 3 cut(s) 150, 214, 376
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 2 cut(s) 211, 401
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 71, 287, 325
BccI CCATC 1 cut(s) 73
BceAI ACGGC 3 cut(s) 201, 351, 396
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 171, 233, 250
BfaI CTAG 2 cut(s) 189, 430
BfoI RGCGCY 2 cut(s) 151, 215
BisI GCNGC 3 cut(s) 60, 301, 339
BlpI GCTNAGC 1 cut(s) 151
BlsI GCNGC 3 cut(s) 61, 302, 340
Bme1390I CCNGG 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 213, 403
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 1 cut(s) 22
BmuI ACTGGG 1 cut(s) 305
Bpu1102I GCTNAGC 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 36
BseMII CTCAG 2 cut(s) 146, 165
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 3 cut(s) 71, 287, 325
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 376
BshNI GGYRCC 2 cut(s) 211, 401
BsiHKAI GWGCWC 1 cut(s) 53
BsmAI GTCTC 3 cut(s) 171, 233, 250
BsmBI CGTCTC 1 cut(s) 233
BsmI GAATGC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 151
BspCNI CTCAG 2 cut(s) 145, 164
BspFNI CGCG 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 213, 403
BspT107I GGYRCC 2 cut(s) 211, 401
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 1 cut(s) 424
BssNI GRCGYC 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 5 cut(s) 19, 127, 244, 329, 401
BstACI GRCGYC 1 cut(s) 212
BstDEI CTNAG 3 cut(s) 6, 132, 151
BstF5I GGATG 1 cut(s) 65
BstFNI CGCG 1 cut(s) 376
BstH2I RGCGCY 2 cut(s) 151, 215
BstHHI GCGC 3 cut(s) 150, 214, 376
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 3 cut(s) 171, 233, 250
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 2 cut(s) 107, 371
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 3 cut(s) 71, 287, 325
BstXI CCANNNNNNTGG 2 cut(s) 118, 286
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 1 cut(s) 334
CfoI GCGC 3 cut(s) 150, 214, 376
Csp6I GTAC 2 cut(s) 402, 414
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviQI GTAC 2 cut(s) 402, 414
DdeI CTNAG 3 cut(s) 6, 132, 151
DinI GGCGCC 1 cut(s) 213
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 278
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 233
FaiI YATR 1 cut(s) 305
Fnu4HI GCNGC 3 cut(s) 60, 301, 339
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 3 cut(s) 60, 301, 339
FspBI CTAG 2 cut(s) 189, 430
GlaI GCGC 3 cut(s) 149, 213, 375
GluI GCNGC 3 cut(s) 60, 301, 339
HaeII RGCGCY 2 cut(s) 151, 215
HhaI GCGC 3 cut(s) 150, 214, 376
Hin1I GRCGYC 1 cut(s) 212
Hin6I GCGC 3 cut(s) 148, 212, 374
HinP1I GCGC 3 cut(s) 148, 212, 374
Hpy166II GTNNAC 2 cut(s) 51, 236
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 2 cut(s) 22, 422
Hpy8I GTNNAC 2 cut(s) 51, 236
Hpy99I CGWCG 3 cut(s) 202, 228, 261
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 5 cut(s) 19, 127, 244, 329, 401
HpyCH4V TGCA 6 cut(s) 35, 51, 59, 303, 325, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 371
HpyF3I CTNAG 3 cut(s) 6, 132, 151
Hsp92I GRCGYC 1 cut(s) 212
HspAI GCGC 3 cut(s) 148, 212, 374
KasI GGCGCC 1 cut(s) 211
KpnI GGTACC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 8 cut(s) 67, 115, 141, 265, 281, 292, 347, 407
Lsp1109I GCAGC 3 cut(s) 71, 287, 325
LweI GCATC 1 cut(s) 22
MaeI CTAG 2 cut(s) 189, 430
MaeIII GTNAC 3 cut(s) 127, 331, 393
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 1 cut(s) 10
Mly113I GGCGCC 1 cut(s) 212
MnlI CCTC 6 cut(s) 96, 107, 228, 264, 272, 359
MspA1I CMGCKG 2 cut(s) 43, 155
MspR9I CCNGG 1 cut(s) 280
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 2 cut(s) 107, 371
NarI GGCGCC 1 cut(s) 212
NdeII GATC 1 cut(s) 424
NlaIV GGNNCC 2 cut(s) 213, 403
NmuCI GTSAC 2 cut(s) 331, 393
PctI GAATGC 1 cut(s) 266
PkrI GCNGC 3 cut(s) 61, 302, 340
PluTI GGCGCC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 2 cut(s) 213, 403
PvuII CAGCTG 2 cut(s) 43, 155
RsaI GTAC 2 cut(s) 403, 415
RsaNI GTAC 2 cut(s) 402, 414
SatI GCNGC 3 cut(s) 60, 301, 339
Sau3AI GATC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 1 cut(s) 53
SetI ASST 5 cut(s) 45, 56, 157, 220, 407
SfaNI GCATC 1 cut(s) 22
SfoI GGCGCC 1 cut(s) 213
Sse9I AATT 1 cut(s) 10
SspDI GGCGCC 1 cut(s) 211
SspMI CTAG 2 cut(s) 189, 430
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 5 cut(s) 19, 127, 244, 329, 401
TaqII GACCGA 1 cut(s) 159
TasI AATT 1 cut(s) 10
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 331, 393
TseI GCWGC 3 cut(s) 59, 300, 338
Tsp45I GTSAC 2 cut(s) 331, 393
TspGWI ACGGA 3 cut(s) 194, 212, 221
TspRI CASTG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 49
XapI RAATTY 1 cut(s) 10
XspI CTAG 2 cut(s) 189, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.