Rroxscaffold_7G00210700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001279
Physical Location & Seq
Forward (+)
61179854 .. 61180983
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00210700.1

Sequence Viewer

Length: 351 bp
ATGGCTTTGCGCTCTGCTGGTTCGCTCTCTCGGTCCTTGGCCTCCACCGCTAGGGTTTCGCTCCACCGCTCCGCCCCAACTCTCCGGCGCCTCCGCCCGCCGACCGCTCCGGCCACTCGCCGCTTCTCCTTCACCTCTCCCAGGAACTTGGGAGAACTGGGATGCACACAGTCGCTGCTGCATATGCGAGCTGCAACATGCCTCACATCTCACCTGACTGTCAATGCGCGTGCTTGTTGCGAGTTGTCCCACGGAATTGGGATTGGGATTGATAGGAAGGAATGGAAAAGATGGGTGATGCGCTTGGACTTACGATCATCCAAAAGGCAGAAGAATGATGCATCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.88

Weight (kDa)

11.75

Isoelectric Point (pI)

74.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AccBSI CCGCTC 2 cut(s) 69, 107
AccII CGCG 1 cut(s) 229
AciI CCGC 7 cut(s) 48, 67, 72, 94, 98, 105, 121
AcoI YGGCCR 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 51, 141
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 1 cut(s) 191
AluI AGCT 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 175
AoxI GGCC 2 cut(s) 39, 111
ApeKI GCWGC 3 cut(s) 175, 178, 191
AspLEI GCGC 4 cut(s) 12, 90, 229, 303
AspS9I GGNCC 1 cut(s) 33
AsuHPI GGTGA 3 cut(s) 124, 203, 307
AvaII GGWCC 1 cut(s) 33
BanI GGYRCC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 162, 165, 178
BccI CCATC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 142
BfaI CTAG 1 cut(s) 51
BfoI RGCGCY 1 cut(s) 91
BisI GCNGC 4 cut(s) 121, 176, 179, 192
BlsI GCNGC 4 cut(s) 122, 177, 180, 193
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 3 cut(s) 152, 288, 328
BmuI ACTGGG 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 36, 140, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 141
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 3 cut(s) 36, 140, 250
BseGI GGATG 2 cut(s) 167, 317
BseLI CCNNNNNNNGG 2 cut(s) 51, 141
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 162, 165, 178
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 41, 113
BshNI GGYRCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 2 cut(s) 85, 110
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 51, 141
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 41, 113
Bsp143I GATC 1 cut(s) 314
BspACI CCGC 7 cut(s) 48, 67, 72, 94, 98, 105, 121
BspANI GGCC 2 cut(s) 41, 113
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 87
BsrBI CCGCTC 2 cut(s) 69, 107
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 3 cut(s) 36, 140, 250
BssMI GATC 1 cut(s) 314
BssNI GRCGYC 1 cut(s) 88
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 171, 220
BstACI GRCGYC 1 cut(s) 88
BstC8I GCNNGC 3 cut(s) 98, 189, 231
BstDSI CCRYGG 1 cut(s) 250
BstENI CCTNNNNNAGG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 167, 317
BstFNI CGCG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 4 cut(s) 12, 90, 229, 303
BstKTI GATC 1 cut(s) 317
BstMBI GATC 1 cut(s) 314
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 47, 184
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 1 cut(s) 140
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 3 cut(s) 162, 165, 178
BstXI CCANNNNNNTGG 2 cut(s) 148, 257
BsuRI GGCC 2 cut(s) 41, 113
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 167, 317
Cac8I GCNNGC 3 cut(s) 98, 189, 231
CaiI CAGNNNCTG 1 cut(s) 175
CfoI GCGC 4 cut(s) 12, 90, 229, 303
Cfr13I GGNCC 1 cut(s) 33
CviAII CATG 1 cut(s) 198
CviJI RGCY 4 cut(s) 5, 41, 113, 191
CviKI_1 RGCY 4 cut(s) 5, 41, 113, 191
DinI GGCGCC 1 cut(s) 89
DpnI GATC 1 cut(s) 316
DpnII GATC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 111
EciI GGCGGA 2 cut(s) 61, 83
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 343
EgeI GGCGCC 1 cut(s) 89
EheI GGCGCC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 1 cut(s) 201
FaiI YATR 3 cut(s) 183, 185, 199
FaqI GGGAC 1 cut(s) 232
FatI CATG 1 cut(s) 197
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 121, 176, 179, 192
FokI GGATG 2 cut(s) 174, 304
Fsp4HI GCNGC 4 cut(s) 121, 176, 179, 192
FspBI CTAG 1 cut(s) 51
GlaI GCGC 4 cut(s) 11, 89, 228, 302
GluI GCNGC 4 cut(s) 121, 176, 179, 192
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 2 cut(s) 41, 113
HapII CCGG 2 cut(s) 85, 110
HhaI GCGC 4 cut(s) 12, 90, 229, 303
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 1 cut(s) 201
Hin6I GCGC 4 cut(s) 10, 88, 227, 301
HinP1I GCGC 4 cut(s) 10, 88, 227, 301
HpaII CCGG 2 cut(s) 85, 110
HphI GGTGA 3 cut(s) 124, 203, 307
HpyAV CCTTC 2 cut(s) 139, 271
HpyCH4III ACNGT 2 cut(s) 171, 220
HpyCH4V TGCA 4 cut(s) 165, 181, 194, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 184
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 1 cut(s) 201
HspAI GCGC 4 cut(s) 10, 88, 227, 301
KasI GGCGCC 1 cut(s) 87
Kzo9I GATC 1 cut(s) 314
LmnI GCTCC 3 cut(s) 66, 74, 112
LpnPI CCDG 7 cut(s) 3, 98, 123, 127, 143, 154, 227
Lsp1109I GCAGC 3 cut(s) 162, 165, 178
LweI GCATC 3 cut(s) 152, 288, 328
MaeI CTAG 1 cut(s) 51
MalI GATC 1 cut(s) 316
MbiI CCGCTC 2 cut(s) 69, 107
MboI GATC 1 cut(s) 314
MboII GAAGA 1 cut(s) 343
MluCI AATT 1 cut(s) 255
Mly113I GGCGCC 1 cut(s) 88
MnlI CCTC 4 cut(s) 52, 101, 145, 212
Mph1103I ATGCAT 1 cut(s) 343
MspI CCGG 2 cut(s) 85, 110
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 47, 184
NarI GGCGCC 1 cut(s) 88
NdeI CATATG 1 cut(s) 183
NdeII GATC 1 cut(s) 314
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 1 cut(s) 89
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 1 cut(s) 201
PkrI GCNGC 4 cut(s) 122, 177, 180, 193
PluTI GGCGCC 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 175
SatI GCNGC 4 cut(s) 121, 176, 179, 192
Sau3AI GATC 1 cut(s) 314
Sau96I GGNCC 1 cut(s) 33
ScrFI CCNGG 1 cut(s) 142
SetI ASST 3 cut(s) 137, 193, 216
SfaNI GCATC 3 cut(s) 152, 288, 328
SfoI GGCGCC 1 cut(s) 89
SinI GGWCC 1 cut(s) 33
Sse9I AATT 1 cut(s) 255
SsiI CCGC 7 cut(s) 48, 67, 72, 94, 98, 105, 121
SspDI GGCGCC 1 cut(s) 87
SspMI CTAG 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 171, 220
TaqII GACCGA 1 cut(s) 21
TasI AATT 1 cut(s) 255
TauI GCSGC 1 cut(s) 123
TseI GCWGC 3 cut(s) 175, 178, 191
TspGWI ACGGA 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 33
XagI CCTNNNNNAGG 1 cut(s) 139
XceI RCATGY 1 cut(s) 201
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.