pycom02g14580

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001279
Physical Location & Seq
Forward (+)
11158462 .. 11158875
414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14580.1

Sequence Viewer

Length: 414 bp
ATGATTTTGCAATTGGAAAGACAACTTCTTCGGTTCCAGGGTTTAAATCGAAATGCCCTAAAACCCTTGCTTCCAATCCAATCCCCAATTCCTAAATTTCCAGCAAGCAAAGGTATGGCGTGGCGATCTGGGTCGTCGTTGTCAAGGTCTCTGATCTCAACCGCAAGAGCTTCGACATTCCGGTCGTCTCCGTCGATCTCATCTCTCCCTCGCGCTCCGCTTCGTAATCTCGTCGGCAGCAACTCCCGCCGCACCCTCTTCTCCCTCCCAGTCAATGCGGGCCAATTAGCTTGCGCGCAGTCGCTGATGCCATTGCACAGCGCCCTGGCTTCGGCTCGTCTCACAAGCCACAGCTCCGACGAAGCGCGGCATTGCTGCGAGCTGTCTCAGGGTACCTCCATTGATCCCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.76

Weight (kDa)

11.66

Isoelectric Point (pI)

74.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 3 cut(s) 213, 296, 367
AciI CCGC 6 cut(s) 162, 218, 247, 250, 278, 367
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 95
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 331
AjnI CCWGG 2 cut(s) 36, 324
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 4 cut(s) 170, 290, 354, 382
AluI AGCT 4 cut(s) 170, 290, 354, 382
Alw26I GTCTC 4 cut(s) 153, 192, 344, 390
AlwI GGATC 1 cut(s) 398
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 237, 375
ApoI RAATTY 1 cut(s) 95
ArsI GACNNNNNNTTYG 2 cut(s) 12, 44
Asp718I GGTACC 1 cut(s) 392
AspLEI GCGC 5 cut(s) 215, 296, 298, 323, 367
AspS9I GGNCC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 249, 362
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 2 cut(s) 38, 326
BcoDI GTCTC 4 cut(s) 153, 192, 344, 390
BfoI RGCGCY 1 cut(s) 324
BisI GCNGC 4 cut(s) 238, 250, 368, 376
BlsI GCNGC 4 cut(s) 239, 251, 369, 377
Bme1390I CCNGG 2 cut(s) 38, 326
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 35, 394
BmrFI CCNGG 2 cut(s) 38, 326
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 37, 324
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 269
Bse3DI GCAATG 2 cut(s) 311, 370
BseBI CCWGG 2 cut(s) 38, 326
BseDI CCNNGG 2 cut(s) 37, 324
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMI GCAATG 2 cut(s) 311, 370
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 269
BsePI GCGCGC 1 cut(s) 294
BseXI GCAGC 2 cut(s) 249, 362
Bsh1236I CGCG 3 cut(s) 213, 296, 367
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 392
BsiEI CGRYCG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 4 cut(s) 153, 192, 344, 390
BsmBI CGTCTC 2 cut(s) 192, 344
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 153
Bsp143I GATC 4 cut(s) 125, 153, 195, 403
BspACI CCGC 6 cut(s) 162, 218, 247, 250, 278, 367
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 1 cut(s) 400
BspFNI CGCG 3 cut(s) 213, 296, 367
BspLI GGNNCC 2 cut(s) 35, 394
BspPI GGATC 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 2 cut(s) 311, 370
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 2 cut(s) 37, 324
BssHII GCGCGC 1 cut(s) 294
BssMI GATC 4 cut(s) 125, 153, 195, 403
Bst2UI CCWGG 2 cut(s) 38, 326
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 5 cut(s) 106, 280, 292, 296, 380
BstDEI CTNAG 1 cut(s) 387
BstFNI CGCG 3 cut(s) 213, 296, 367
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 5 cut(s) 215, 296, 298, 323, 367
BstKTI GATC 4 cut(s) 128, 156, 198, 406
BstMAI GTCTC 4 cut(s) 153, 192, 344, 390
BstMBI GATC 4 cut(s) 125, 153, 195, 403
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 2 cut(s) 38, 326
BstSCI CCNGG 2 cut(s) 36, 324
BstUI CGCG 3 cut(s) 213, 296, 367
BstV1I GCAGC 2 cut(s) 249, 362
BsuRI GGCC 1 cut(s) 282
Cac8I GCNNGC 5 cut(s) 106, 280, 292, 296, 380
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 5 cut(s) 215, 296, 298, 323, 367
Cfr13I GGNCC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 393
CviJI RGCY 8 cut(s) 170, 282, 290, 329, 335, 348, 354, 382
CviKI_1 RGCY 8 cut(s) 170, 282, 290, 329, 335, 348, 354, 382
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 127, 155, 197, 405
DpnII GATC 4 cut(s) 125, 153, 195, 403
DraI TTTAAA 1 cut(s) 45
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco31I GGTCTC 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 36, 324
Esp3I CGTCTC 2 cut(s) 192, 344
FaiI YATR 1 cut(s) 116
FauI CCCGC 2 cut(s) 254, 271
Fnu4HI GCNGC 4 cut(s) 238, 250, 368, 376
Fsp4HI GCNGC 4 cut(s) 238, 250, 368, 376
GlaI GCGC 5 cut(s) 214, 295, 297, 322, 366
GluI GCNGC 4 cut(s) 238, 250, 368, 376
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 181
HhaI GCGC 5 cut(s) 215, 296, 298, 323, 367
Hin6I GCGC 5 cut(s) 213, 294, 296, 321, 365
HinP1I GCGC 5 cut(s) 213, 294, 296, 321, 365
HpaII CCGG 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 153, 358
Hpy99I CGWCG 4 cut(s) 139, 196, 236, 362
HpyCH4V TGCA 2 cut(s) 10, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 387
HspAI GCGC 5 cut(s) 213, 294, 296, 321, 365
KpnI GGTACC 1 cut(s) 396
Kzo9I GATC 4 cut(s) 125, 153, 195, 403
LmnI GCTCC 2 cut(s) 220, 359
LpnPI CCDG 9 cut(s) 23, 50, 114, 114, 194, 282, 311, 338, 374
Lsp1109I GCAGC 2 cut(s) 249, 362
LweI GCATC 1 cut(s) 297
MalI GATC 4 cut(s) 127, 155, 197, 405
MboI GATC 4 cut(s) 125, 153, 195, 403
MboII GAAGA 2 cut(s) 20, 250
MfeI CAATTG 1 cut(s) 11
MluCI AATT 4 cut(s) 11, 87, 95, 284
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 4 cut(s) 219, 266, 275, 406
MseI TTAA 1 cut(s) 44
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 2 cut(s) 38, 326
MunI CAATTG 1 cut(s) 11
MvaI CCWGG 2 cut(s) 38, 326
MvnI CGCG 3 cut(s) 213, 296, 367
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 4 cut(s) 125, 153, 195, 403
NlaIV GGNNCC 2 cut(s) 35, 394
PauI GCGCGC 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PkrI GCNGC 4 cut(s) 239, 251, 369, 377
Psp6I CCWGG 2 cut(s) 36, 324
PspGI CCWGG 2 cut(s) 36, 324
PspN4I GGNNCC 2 cut(s) 35, 394
PspPI GGNCC 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 304
PteI GCGCGC 1 cut(s) 294
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 4 cut(s) 238, 250, 368, 376
Sau3AI GATC 4 cut(s) 125, 153, 195, 403
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 38, 326
SetI ASST 7 cut(s) 115, 149, 172, 292, 356, 384, 398
SfaNI GCATC 1 cut(s) 297
Sse9I AATT 4 cut(s) 11, 87, 95, 284
SsiI CCGC 6 cut(s) 162, 218, 247, 250, 278, 367
StyD4I CCNGG 2 cut(s) 36, 324
TaqI TCGA 3 cut(s) 49, 173, 194
TasI AATT 4 cut(s) 11, 87, 95, 284
TauI GCSGC 2 cut(s) 252, 370
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TseI GCWGC 2 cut(s) 237, 375
TspGWI ACGGA 1 cut(s) 180
XapI RAATTY 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.